745,254 results match your criteria strains adopting

Design, synthesis and anti-HIV evaluation of novel 5-substituted diarylpyrimidine derivatives as potent HIV-1 NNRTIs.

Bioorg Med Chem 2021 May 5;40:116195. Epub 2021 May 5.

Department of Medicinal Chemistry, Key Laboratory of Chemical Biology, Ministry of Education, School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Ji'nan 250012, China; China-Belgium Collaborative Research Center for Innovative Antiviral Drugs of Shandong Province, 44 West Culture Road, 250012 Jinan, Shandong, PR China. Electronic address:

Non-nucleoside reverse transcriptase inhibitors (NNRTIs) are widely used in combination therapies against HIV-1. As a continuation of our efforts to discover and develop "me-better" drugs of DAPYs, novel diarylpyrimidine derivatives were designed, synthesized and evaluated for their anti-HIV activities in MT-4 cells. All the compounds demonstrated strong inhibition activity against wide-type HIV-1 strain (III) with EC values in the range of 2. Read More

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Feeding olive flounder (Paralichthys olivaceus) with Lactococcus lactis BFE920 expressing the fusion antigen of Vibrio OmpK and FlaB provides protection against multiple Vibrio pathogens: a universal vaccine effect.

Fish Shellfish Immunol 2021 May 9. Epub 2021 May 9.

School of Life Science, Handong University, 558 Handong-ro, Pohang-city, Gyeongbuk, 37554, South Korea. Electronic address:

Vibriosis, an illness caused by the Vibrio bacteria species, results in significant economic loss in olive flounder farms. Here we present a novel anti-Vibrio feed vaccine protecting multiple strains of Vibrio pathogens, a universal vaccine effect. The vaccine was generated by engineering Lactococcus lactis BFE920 to express the fusion antigens of Vibrio outer membrane protein K (OmpK) and flagellin B subunit (FlaB). Read More

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Targeting SUMOylation dependency in human cancer stem cells through a unique SAE2 motif revealed by chemical genomics.

Cell Chem Biol 2021 May 7. Epub 2021 May 7.

Stem Cell and Cancer Research Institute, McMaster University, Hamilton, ON L8S 4K1, Canada; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4K1, Canada. Electronic address:

Natural products (NPs) encompass a rich source of bioactive chemical entities. Here, we used human cancer stem cells (CSCs) in a chemical genomics campaign with NP chemical space to interrogate extracts from diverse strains of actinomycete for anti-cancer properties. We identified a compound (McM25044) capable of selectively inhibiting human CSC function versus normal stem cell counterparts. Read More

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Interplay between H1N1 influenza a virus infection, extracellular and intracellular respiratory tract pH, and host responses in a mouse model.

PLoS One 2021 12;16(5):e0251473. Epub 2021 May 12.

Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, United States of America.

During influenza A virus (IAV) entry, the hemagglutinin (HA) protein is triggered by endosomal low pH to undergo irreversible structural changes that mediate membrane fusion. HA proteins from different isolates vary in the pH at which they become activated in endosomes or become irreversible inactivated if exposed to extracellular acid. Little is known about extracellular pH in the upper respiratory tracts of mammals, how pH may shift during IAV infection, and its impact on replication of viruses that vary in HA activation pH. Read More

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Discovery of human ACE2 variants with altered recognition by the SARS-CoV-2 spike protein.

PLoS One 2021 12;16(5):e0251585. Epub 2021 May 12.

Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, United States of America.

Understanding how human ACE2 genetic variants differ in their recognition by SARS-CoV-2 can facilitate the leveraging of ACE2 as an axis for treating and preventing COVID-19. In this work, we experimentally interrogate thousands of ACE2 mutants to identify over one hundred human single-nucleotide variants (SNVs) that are likely to have altered recognition by the virus, and make the complementary discovery that ACE2 residues distant from the spike interface influence the ACE2-spike interaction. These findings illuminate new links between ACE2 sequence and spike recognition, and could find substantial utility in further fundamental research that augments epidemiological analyses and clinical trial design in the contexts of both existing strains of SARS-CoV-2 and novel variants that may arise in the future. Read More

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The duration of antibiotic treatment is associated with carriage of toxigenic and non-toxigenic strains of Clostridioides difficile in dogs.

PLoS One 2021 12;16(5):e0245949. Epub 2021 May 12.

Veterinary Pathology, Public Health and Disease Investigation, School of Veterinary Medicine, University of Glasgow, Glasgow, United Kingdom.

Clostridioides difficile is a leading cause of human antibiotic-associated diarrhoeal disease globally. Zoonotic reservoirs of infection are increasingly suspected to play a role in the emergence of this disease in the community and dogs are considered as one potential source. Here we use a canine case-control study at a referral veterinary hospital in Scotland to assess: i) the risk factors associated with carriage of C. Read More

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STAT2-dependent restriction of Zika virus by human macrophages but not dendritic cells.

Emerg Microbes Infect 2021 May 12:1-42. Epub 2021 May 12.

State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China.

Zika virus (ZIKV) is an emerging mosquito-borne flavivirus that poses significant threats to global public health. Macrophages and dendritic cells are both key sentinel cells in the host immune response and play critical roles in the pathogenesis of flavivirus infections. Recent studies showed that ZIKV could productively infect monocyte-derived dendritic cells (moDCs), but the role of macrophages in ZIKV infection remains incompletely understood. Read More

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Internal ballistics of polygonal and grooved barrels: A comparative study.

Sci Prog 2021 Apr-Jun;104(2):368504211016954

Instituto Politécnico Nacional, SEPI-ESIME U.P., Zacatenco, Ciudad de México, México.

As a parameter important ballistic, the research about polygonal and grooved barrels' behavior has not been widely carried out. The pressures, velocities, stresses, deformations, and strains generated by the firing of 9 mm × 19 mm ammunition in weapons with polygonal barrels are analyzed numerically and experimentally, compared with those generated in pistols with grooved barrels. The Finite Element Method with equal boundary and loading conditions was used in both types of guns, specifying the actual materials of the projectile and the barrels. Read More

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High-Throughput and Continuous Chaotic Bioprinting of Spatially Controlled Bacterial Microcosms.

ACS Biomater Sci Eng 2021 May 12. Epub 2021 May 12.

Centro de Biotecnología-FEMSA, Tecnologico de Monterrey, Monterrey, Nuevo Leon 64849, México.

Microorganisms do not work alone but instead function as collaborative microsocieties. The spatial distribution of different bacterial strains (micro-biogeography) in a shared volumetric space and their degree of intimacy greatly influences their societal behavior. Current microbiological techniques are commonly focused on the culture of well-mixed bacterial communities and fail to reproduce the micro-biogeography of polybacterial societies. Read More

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Systems genetic analysis of binge-like eating in a C57BL/6J x DBA/2J-F2 cross.

Genes Brain Behav 2021 May 12:e12751. Epub 2021 May 12.

Laboratory of Addiction Genetics, Department of Pharmacology and Experimental Therapeutics and Psychiatry, Boston University School of Medicine, Boston, MA, USA.

Binge eating is a heritable trait associated with eating disorders and refers to the rapid consumption of a large quantity of energy-dense food that is associated with loss of control and negative affect. Binge Eating Disorder is the most common eating disorder in the US; however, the genetic basis is unknown. We previously identified robust mouse inbred strain differences between C57BL/6J and DBA/2J in binge-like eating of sweetened palatable food in an intermittent access, conditioned place preference paradigm (PF-CPP). Read More

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Enriched Opportunistic Pathogens Revealed by Metagenomic Sequencing Hint Potential Linkages between Pharyngeal Microbiota and COVID-19.

Virol Sin 2021 May 12. Epub 2021 May 12.

CAS Key Laboratory of Special Pathogens and Biosafety, Centre for Biosafety Mega-Sciences, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.

As a respiratory tract virus, SARS-CoV-2 infected people through contacting with the upper respiratory tract first. Previous studies indicated that microbiota could modulate immune response against pathogen infection. In the present study, we performed metagenomic sequencing of pharyngeal swabs from eleven patients with COVID-19 and eleven Non-COVID-19 patients who had similar symptoms such as fever and cough. Read More

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Pseudomonas chlororaphis metabolites as biocontrol promoters of plant health and improved crop yield.

World J Microbiol Biotechnol 2021 May 12;37(6):99. Epub 2021 May 12.

Center Agriculture Food Environment C3A, University of Trento/Fondazione Edmund Mach, San Michele all'Adige, TN, Italy.

The Pseudomonas fluorescens complex contains at least eight phylogenetic groups and each of these includes several bacterial species sharing ecological and physiological traits. Pseudomonas chlororaphis classified in a separate group is represented by three different subspecies that show distinctive traits exploitable for phytostimulation and biocontrol of phytopathogens. The high level of microbial competitiveness in soil as well as the effectiveness in controlling several plant pathogens and pests can be related to the P. Read More

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Multiple system atrophy-associated oligodendroglial protein p25α stimulates formation of novel α-synuclein strain with enhanced neurodegenerative potential.

Acta Neuropathol 2021 May 12. Epub 2021 May 12.

DANDRITE, Danish Research Institute of Translational Neuroscience & Department of Biomedicine, Aarhus University, 8000, Aarhus C, Denmark.

Pathology consisting of intracellular aggregates of alpha-Synuclein (α-Syn) spread through the nervous system in a variety of neurodegenerative disorders including Parkinson's disease, dementia with Lewy bodies, and multiple system atrophy. The discovery of structurally distinct α-Syn polymorphs, so-called strains, supports a hypothesis where strain-specific structures are templated into aggregates formed by native α-Syn. These distinct strains are hypothesised to dictate the spreading of pathology in the tissue and the cellular impact of the aggregates, thereby contributing to the variety of clinical phenotypes. Read More

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Understanding the Meaning of Recovery to Patients Undergoing Abdominal Surgery.

JAMA Surg 2021 May 12. Epub 2021 May 12.

Steinberg-Bernstein Centre for Minimally Invasive Surgery and Innovation, McGill University Health Centre, Montreal, Québec, Canada.

Importance: Postoperative recovery is difficult to define or measure. Research addressing interventions aimed to improve recovery after abdominal surgery often focuses on measures such as duration of hospital stay and complication rates. Although these clinical parameters are relevant, understanding patients' perspectives regarding postoperative recovery is fundamental to guiding patient-centered care. Read More

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Mitochondrial activities play a pivotal role in regulating cell cycle in response to doxorubicin.

Cell Cycle 2021 May 12:1-13. Epub 2021 May 12.

Department of Biomedical Science, University of Minnesota Medical School, Duluth Campus, USA.

Doxorubicin induces both DNA damage and metabolic interference. How these effects interact to modulate cellular toxicity is not completely understood but important given the widespread use of doxorubicin in cancer treatment. This study tests the hypothesis that cell cycle arrest and survival are affected by distinct mitochondrial activities during doxorubicin exposure. Read More

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Genetic Dissection of T-DNA Insertional Mutants Reveals Uncoupling of Dikaryotic Filamentation and Virulence in Sugarcane Smut Fungus.

Phytopathology 2021 May 12. Epub 2021 May 12.

Guangxi University, 12664, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources , Nanning, Guangxi, China.

The biotrophic basidiomycetous fungus causing smut disease in sugarcane is characterized by a life-cycle composed of a yeast-like nonpathogenic haploid basidiosporial stage outside the plant and filamentous pathogenic dikaryotic hyphae within the plant. Under field conditions, dikaryotic hyphae are formed after mating of two opposite mating-type strains. However, the mechanisms underlying genetic regulation of filamentation and its association with pathogenicity and development of teliospores are currently unclear. Read More

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Detection and genetic analysis of a novel atypical porcine pestivirus from piglets with congenital tremor in Japan.

Transbound Emerg Dis 2021 May 12. Epub 2021 May 12.

School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, 252-5201, Japan.

Atypical porcine pestivirus (APPV), which has been confirmed to be associated with congenital tremor (CT) in pigs, is a newly discovered porcine virus that has been found in the Americas, Europe, and Asia; however, no report of APPV in Japan has been published. We identified an APPV in the central nervous system of Japanese piglets with CT, and firstly determined and analyzed the complete genome sequence. Phylogenetic analysis using the complete genome nucleotide sequence of the Japanese APPV, named Anna/2020, and those of APPVs from the NCBI database showed that APPVs were divided into three genotypes (genotypes 1 to 3), and that Anna/2020 clustered with the genotype 3 APPV strains, but distantly branched from these strains. Read More

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Progress of genome editing technology and developmental biology useful for radiation research.

J Radiat Res 2021 May;62(Supplement_1):i53-i63

Department of Disease Model, Research Institute of Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553, Japan.

Following the development of genome editing technology, it has become more feasible to create genetically modified animals such as knockout (KO), knock-in, and point-mutated animals. The genome-edited animals are useful to investigate the roles of various functional genes in many fields of biological science including radiation research. Nevertheless, some researchers may experience difficulty in generating genome-edited animals, probably due to the requirement for equipment and techniques for embryo manipulation and handling. Read More

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Late effects of Beauveria bassiana on larval stages of Aedes aegypti Linneo‎, ‎1762‎ (Diptera: Culicidae).

Braz J Biol 2021 10;82:e237789. Epub 2021 May 10.

Universidad Autónoma de Nuevo León, Instituto de Biotecnología, Facultad de Ciencias Biológicas, San Nicolás de los Garza, N.L. México.

Aedes aegypti is a culicide that has gained relevance over the years due to its ability to transmit various viruses that cause diseases in humans that all the years cause high mortality rates in the world population. The main problem is that Ae. aegypti has managed to establish and maintain a close relationship with humans and their habitat, which is why the search for alternatives to control vector populations becomes imperative. Read More

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FJSYC4-1 and FGSZY33L6 alleviate metabolic syndrome gut microbiota regulation.

Food Funct 2021 May;12(9):3919-3930

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China. and School of Food Science and Technology, Jiangnan University, Wuxi, China and National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, China and Beijing Innovation Center of Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing, China.

Metabolic syndrome, which includes a series of metabolic disorders such as hyperglycemia, hyperlipidemia, insulin resistance and obesity, has become a catastrophic disease worldwide. Accordingly, probiotic intervention is a new strategy to alleviate metabolic syndrome, which can adjust the gut microbiota to a certain extent. The aim of the current work was to explore the alleviation of metabolic syndrome by Lactobacillus reuteri and L. Read More

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Characterization of R-pyocin activity against Gram-positive pathogens for the first time with special focus on Staphylococcus aureus.

J Appl Microbiol 2021 May 11. Epub 2021 May 11.

Department of Microbiology and Botany, Faculty of Science, Zagazig University, 44519, Zagazig, Egypt.

Aim: This study is aimed at characterization of both antimicrobial and anti-biofilm activity of R-pyocin from clinical Pseudomonas aeruginosa against Gram-positive pathogens including Staphylococcus aureus.

Methods And Results: Pyocinogenic P. aeruginosa was detected using reverse-side method and pyocinogeny typing was confirmed using revised-spotting method. Read More

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Antibiotics Therapy in the Multi-Resistant Patient.

Methods Mol Biol 2021 ;2296:423-439

Faculty of Health Sciences, University of León, León, Spain.

Antibiotic resistance is not only a European or American problem: it is a global crisis. The emergence of resistance to multiple antimicrobial agents has become a major threat to public health. The main objective of the institution's antimicrobial policy is to adapt the effective treatment to each patient, with a minimum of complications, avoid adverse reactions, control the development and spread of strains of resistant microorganisms as well as reduce hospital costs whenever possible. Read More

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January 2021

Production, Detection, Extraction, and Quantification of Polyene Antibiotics.

Methods Mol Biol 2021 ;2296:333-350

Area of Microbiology, Faculty of Biology, University of León, León, Spain.

Polyene antibiotics are macrolide antifungal compounds obtained by fermentation of producer Streptomyces strains. Here we describe commonly used methods for polyene production, detection, and their subsequent extraction and purification. While bioassays are used to detect these compounds based on their biological activity, quantification by spectrophotometry or high-performance liquid chromatography (HPLC ) relies on their physiochemical properties and is more reliable. Read More

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January 2021

Molecular Biology Methods in Streptomyces rimosus, a Producer of Oxytetracycline.

Methods Mol Biol 2021 ;2296:303-330

Chair of Biotechnology, Microbiology and Food Safety, Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.

Streptomyces rimosus is used for production of the broad-spectrum antibiotic oxytetracycline (OTC). S. rimosus belongs to Actinomyces species, a large group of microorganisms that produce diverse set of natural metabolites of high importance in many aspects of our life. Read More

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January 2021

Novel Methods to Identify Oxidative Stress-Producing Antibiotics.

Methods Mol Biol 2021 ;2296:249-261

Department of Molecular Biology, Area of Microbiology, University of León, León, Spain.

Antibiotherapy is the main therapeutic strategy in the fight against bacterial pathogens. However, the misuse of antimicrobials has led to the appearance of antimicrobial-resistant strains. The rate at which we isolate multidrug-resistant bacteria is now much faster than the discovery rate of new antimicrobials. Read More

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January 2021

A Simple Antifungal assay for Testing Actinomycetes and Other Microbial Extracts.

Methods Mol Biol 2021 ;2296:217-225

Bacteriology Department, Biomar Microbial Technologies S.A., León, Spain.

Antifungal assay in vitro is a useful tool for the characterization of biological activity of microbial extracts. Here we describe a simple in vitro test at two final extract concentrations that allows long-term storage of the plates containing dry extracts before using. The assay protocol is described for two fungal strains, a unicellular yeast, with clinical interest (Candida albicans), and a sporulated and phytopathogenic filamentous fungus (Botrytis cinerea). Read More

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January 2021

High-Efficiency Electroporation for Genetic Improvement of Fungal Strains.

Methods Mol Biol 2021 ;2296:185-194

Department of Biotechnology, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City, Mexico.

Electroporation is a method for the introduction of molecules (usually nucleic acids) into a cell, consisting of submitting the cells to high-voltage and short electric pulses in the presence of the exogenous DNA/molecule. It is a versatile method, adaptable to different types of cells, from bacteria to cultured cells to higher eukaryotes, and thus has applications in many diverse fields, such as environmental biology, biotechnology, genetic engineering, and medicine. Electroporation has some advantages over other genetic transformation strategies, including the simplicity of the method, a wide range of adjustable parameters (possibility of optimization), high reproducibility and avoidance of the use of chemicals toxic to cells. Read More

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January 2021

Screening Systems for Stable Markerless Genomic Deletions/Integrations in Streptomyces Species.

Methods Mol Biol 2021 ;2296:91-141

Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovak Republic.

Bacteria of the genus Streptomyces are one of the most important producers of biologically active natural products. Recent robust genomic sequencing of Streptomyces strains has shown enormous genetic potential for new natural products. However, many biosynthetic gene clusters are silent. Read More

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January 2021

Dilution-to-Extinction Platform for the Isolation of Marine Bacteria-Producing Antitumor Compounds.

Methods Mol Biol 2021 ;2296:77-87

Research and Development Department, PharmaMar S.A., Madrid, Spain.

Gram-negative marine bacteria are an underexplored source of new chemical entities for a wide range of applications. Even though, some have shown a high antitumor activity. This chapter describes an isolation and screening protocol based on the Dilution-to-Extinction approach coupled with an antiproliferative test oriented to the discovery of new cytotoxic compounds synthesized by marine bacteria. Read More

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January 2021

Characterizationharacterization of Extremophilic Actinomycetes Strains as Sources of Antimicrobial Agents.

Methods Mol Biol 2021 ;2296:59-75

Nazarbayev University School of Medicine (NUSOM), Nur-Sultan, Kazakhstan.

Extremophilic actinomycetes strains can survive extreme saline and alkaline environments and produce antimicrobial agents. In this chapter, we discuss laboratory methods that can be used to isolate and characterize actinomycetes strains capable of potentially producing novel antimicrobial agent(s) when cultured in conditions that mimic the environments from which they were isolated. Methods used to screen for antibacterial and antiviral activities from these producer strains, and microbiological and molecular approaches used to identify these strains are described. Read More

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January 2021