760,735 results match your criteria strains


Chemical composition and antibacterial activity of 12 medicinal plant ethyl acetate extracts using LC-MS feature-based molecular networking.

Phytochem Anal 2022 Jan 18. Epub 2022 Jan 18.

Natural Products Laboratory, State Key Laboratory of Tea Plant Biology and Utilisation, Anhui Agricultural University, Hefei, P. R. China.

Introduction: Widespread use of antibiotics has led to an increase in bacterial multiple drug resistance, thereby searching for natural antimicrobial agents from plants becomes an effective and alternative approach. In the present study, we selected six foodborne bacteria to evaluate the antibacterial activities of 12 medicinal plants ethyl acetate (EA) extracts.

Objective: This study aims to search for natural antibiotic substitutes from plant extracts. Read More

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January 2022

Evaluation of the Genotoxic Potential of the Selective COX-2 Inhibitor Enflicoxib in a Battery of in vitro and in vivo Genotoxicity Assays.

Drug Res (Stuttg) 2022 Jan 18. Epub 2022 Jan 18.

Ecuphar Veterinaria S.L.U. (Animalcare Group). Avgda. Río de Janeiro 60 - 66, planta 13, Barcelona - Spain.

Aim: Enflicoxib, a selective COX-2 inhibitor approved for the treatment of pain and inflammation associated with osteoarthritis in dogs (Daxocox [Ecuphar/Animalcare Group]) was assessed for its genotoxic potential in a battery of and genotoxicity assays. These comprised a bacterial reverse mutation assay (Ames test), an human lymphocyte chromosome aberration assay and an mouse bone marrow micronucleus assay.

Methods: Relevant vehicle and positive control cultures and animals were included in all assays. Read More

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January 2022

In vitro susceptibility of human gut microbes to potential food preservatives based on immobilized phenolic compounds.

Food Chem 2022 Jan 15;378:132136. Epub 2022 Jan 15.

Departamento de Tecnología de Alimentos, Universitat Politècnica de València, Valencia, Spain.

The development of novel food preservatives based on natural antimicrobials such as phenolic compounds is increasing, but their safety should be established before use, including evaluating their impact on the gut microbiota. This work explored the influence of antimicrobial phenolics presented in different forms on selected human gut microbiota members through in vitro susceptibility tests. The bacteria tested exhibited a wide range of susceptibilities to phenolics depending on the molecule structure and mode of administration. Read More

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January 2022

Impacts of selected lactic acid bacteria strains on the aroma and bioactive compositions of fermented gilaburu (Viburnum opulus) juices.

Food Chem 2022 Jan 7;378:132079. Epub 2022 Jan 7.

Department of Food Engineering, Faculty of Engineering, Adana Alparslan Turkes Science and Technology University, 01250 Adana, Turkey. Electronic address:

Gilaburu fruit and its products have gained popularity due to their nutritional content, taste and health benefits. Even though fermented gilaburu juice is widely preferred and consumed in some regions, there is no detailed study on the optimization of the production conditions of this popular beverage. In this study, gilaburu fruit juices fermented naturally (NFJ) and with three commercial lactic acid bacteria (LAB) (L. Read More

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January 2022

The SARS-CoV-2 mutations versus vaccine effectiveness: New opportunities to new challenges.

J Infect Public Health 2022 Jan 5;15(2):228-240. Epub 2022 Jan 5.

Department of Pharmacology & Toxicology, College of Pharmacy, University of Hail, Hail, Saudi Arabia. Electronic address:

Background: The SARS-CoV-2 coronavirus epidemic is hastening the discovery of the most efficient vaccines. The development of cost-effective vaccines seems to be the only solution to terminate this pandemic. However, the vaccines' effectiveness has been questioned due to recurrent mutations in the SARS-CoV-2 genome. Read More

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January 2022

Fungal endophytes in Fraxinus excelsior petioles and their in vitro antagonistic potential against the ash dieback pathogen Hymenoscyphus fraxineus.

Microbiol Res 2022 Jan 4;257:126961. Epub 2022 Jan 4.

Department of Forest Ecosystem Protection, Faculty of Forestry, University of Agriculture in Cracow, 31-425 Cracow, Al. 29-Listopada 46, Poland. Electronic address:

Fungal endophytes were isolated from 250 asymptomatic leaf petioles of Fraxinus excelsior collected from trees showing symptoms of ash dieback in five forest sites in southern Poland. Fungal isolations yielded 1646 colonies representing 97 taxa, including 92 Ascomycota and 5 Basidiomycota species. The most common Ascomycota comprised Nemania serpens (38. Read More

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January 2022

4Antimicrobial photodynamic therapy with curcumin on methicillin-resistant Staphylococcus aureus biofilm.

Photodiagnosis Photodyn Ther 2022 Jan 16:102729. Epub 2022 Jan 16.

Laboratory of Photobiology Applied to Health (FOTOBIOS), Research and Development Institute (IP&D), University of Vale do Paraíba (UNIVAP), São José dos Campos, São Paulo, Brazil. Electronic address:

Healthcare-Associated Infections (HAI) affect approximately 1.5 million individuals worldwide. Among the causes of HAIs in Latin America, Staphylococcus aureus presents a severe danger due to its rapid spread and ease of developing antibiotic resistance. Read More

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January 2022

Evaluation of bacterial agents isolated from endotracheal aspirate cultures of Covid-19 general intensive care patients and their antibiotic resistance profiles compared to pre-pandemic conditions.

Microb Pathog 2022 Jan 15:105409. Epub 2022 Jan 15.

Siirt University, Medical Faculty, Department of Internal Medicine, 56100, Siirt, Turkey.

Background: Early reports have shown that critically ill patients infected with SARS-CoV-2 have a high prevalence of nosocomial pneumonia, particularly ventilator-associated pneumonia (VAP).

Method: In the present study, we determined the bacterial agents isolated in endotracheal aspirate (ETA) cultures of Covid-19 general intensive care patients and evaluated the antibiotic resistance profiles of common bacterial agents compared to the pre-pandemic period.

Results: While a total of 119 significant growths with polymicrobial growths were detected in the ETA cultures of 73 (7. Read More

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January 2022

Lentinan extends lifespan and increases oxidative stress resistance through DAF-16 and SKN-1 pathways in Caenorhabditis elegans.

Int J Biol Macromol 2022 Jan 15. Epub 2022 Jan 15.

Key Laboratory of Pesticide Toxicology & Application Technique, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, China. Electronic address:

Lentinan, extracted from Lentinus edodes, exhibits bioactive properties in vitro; however, little is known about the antioxidant potential in vivo. In this study, the effects of lentinan at 0.05, 0. Read More

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January 2022

A European multicenter evaluation study to investigate the performance on commercially available selective agar plates for the detection of carbapenemase producing Enterobacteriaceae.

J Microbiol Methods 2022 Jan 15:106418. Epub 2022 Jan 15.

Norwegian Veterinary Institute, P.O. Box 64, 1431 Ås, Norway. Electronic address:

The European Food Safety Authority (EFSA) advised to prioritize monitoring carbapenemase producing Enterobacteriaceae (CPE) in food producing animals. Therefore, this study evaluated the performance of different commercially available selective agars for the detection of CPE using spiked pig caecal and turkey meat samples and the proposed EFSA cultivation protocol. Eleven laboratories from nine countries received eight samples (four caecal and four meat samples). Read More

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January 2022

Size-dependent visible-light-enhanced Cr(VI) bioreduction by hematite nanoparticles.

Chemosphere 2022 Jan 15:133633. Epub 2022 Jan 15.

College of Resources and Environment, Huazhong Agricultural University, Wuhan, 430070, PR China.

Light irradiation would affect the electron transfer between dissimilatory metal-reducing bacteria (DMRB) and semiconducting minerals, which may impose a great influence on the biogeochemistry cycle of heavy metals. However, the size effect of semiconducting minerals on the its electron transfer with DMRB and microbial Cr(VI) reduction under visible light irradiation is little known. Herein, the Cr(VI) reduction by Shewanella oneidensis MR-1 (MR-1) was investigated in the presence of hematite nanoparticles with average diameters of 10 nm and 50 nm in dark and under visible light irradiation. Read More

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January 2022

A quantitative, high-throughput urease activity assay for comparison and rapid screening of ureolytic bacteria.

Environ Res 2022 Jan 15:112738. Epub 2022 Jan 15.

School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Ave, Singapore, 639798, Singapore; Singapore Centre for Environmental Life Sciences Engineering, Interdisciplinary Graduate Programme, Graduate College, Nanyang Technological University, 60 Nanyang Dr, Singapore, 637551, Singapore. Electronic address:

Urease is a dinickel enzyme commonly found in numerous organisms that catalyses the hydrolysis of urea into ammonia and carbon dioxide. The microbially induced carbonate precipitation (MICP) process medicated by urease-producing bacteria (UPB) can be used for many applications including, environmental bioremediation, soil improvement, healing of cracks in concrete, and sealing of rock joints. Despite the importance of urease and UPB in various applications, a quantitative, high-throughput assay for the comparison of urease activity in UPB and rapid screening of UPB from diverse environments is lacking. Read More

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January 2022

Complete sequences of epidermin and nukacin encoding plasmids from oral-derived Staphylococcus epidermidis and their antibacterial activity.

PLoS One 2022 18;17(1):e0258283. Epub 2022 Jan 18.

Department of Bacteriology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan.

Staphylococcus epidermidis is a commensal bacterium in humans. To persist in the bacterial flora of the host, some bacteria produce antibacterial factors such as the antimicrobial peptides known as bacteriocins. In this study, we tried to isolate bacteriocin-producing S. Read More

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January 2022

Integrating tuberculosis and noncommunicable diseases care in low- and middle-income countries (LMICs): A systematic review.

PLoS Med 2022 Jan 18;19(1):e1003899. Epub 2022 Jan 18.

Saw Swee Hock School of Public Health, National University of Singapore, Singapore.

Background: Low- and middle-income countries (LMICs) are facing a combined affliction from both tuberculosis (TB) and noncommunicable diseases (NCDs), which threatens population health and further strains the already stressed health systems. Integrating services for TB and NCDs is advantageous in tackling this joint burden of diseases effectively. Therefore, this systematic review explores the mechanisms for service integration for TB and NCDs and elucidates the facilitators and barriers for implementing integrated service models in LMIC settings. Read More

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January 2022

Factors associated with breakthrough fungemia caused by , , or species when the breakthrough fungemia developed in patients with hematological disorders.

Antimicrob Agents Chemother 2022 Jan 18:AAC0208121. Epub 2022 Jan 18.

Department of Infectious Diseases, Toranomon Hospital, Tokyo, Japan.

Limited data are available on breakthrough fungemia, defined as fungemia that develops on administration of antifungal agents, in patients with hematological disorders. We reviewed the medical and microbiological records of adult patients with hematological diseases who had breakthrough fungemia between January 2008 and July 2019 at Toranomon Hospital and Toranomon Hospital Kajigaya in Japan. A total of 121 cases of breakthrough fungemia were identified. Read More

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January 2022

Transcriptomics reveals how minocycline-colistin synergy overcomes antibiotic resistance in multidrug-resistant .

Antimicrob Agents Chemother 2022 Jan 18:aac0196921. Epub 2022 Jan 18.

Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.

Multidrug resistant gram-negative bacteria are a rapidly growing public health threat, and the development of novel antimicrobials has failed to keep pace with their emergence. Synergistic combinations of individually ineffective drugs present a potential solution, yet little is understood about the mechanisms of most such combinations. Here, we show that the combination of colistin (polymyxin E) and minocycline has a high rate of synergy against colistin-resistant and minocycline-intermediate or -resistant strains of . Read More

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January 2022

Simulated intravenous inhaled tobramycin with and without intravenous ceftazidime evaluated against hypermutable a dynamic biofilm model and mechanism-based modeling.

Antimicrob Agents Chemother 2022 Jan 18:aac0220321. Epub 2022 Jan 18.

Centre for Medicine Use and Safety, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Victoria, Australia.

Acute exacerbations of chronic respiratory infections in patients with cystic fibrosis are highly challenging due to hypermutable , biofilm formation and resistance emergence. We aimed to systematically evaluate the effects of intravenous inhaled tobramycin with and without intravenous ceftazidime. Two hypermutable isolates, CW30 (MIC 0. Read More

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January 2022

Palmarumycin P3 reverses Mrr1-mediated azole resistance by blocking the efflux pump Mdr1.

Antimicrob Agents Chemother 2022 Jan 18:aac0212621. Epub 2022 Jan 18.

Department of Natural Product Chemistry, Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China.

Palmarumycin P3 (PP3) reduces fluconazole-induced transcription to reverse azole resistance in clinical strains. Here, we demonstrated that PP3 restores the susceptibility of strains with gain-of-function mutations in the transcription factor Mrr1 to several antifungal drugs. In addition, PP3 inhibits the efflux of Mdr1 substrates by strains harbouring hyperactive alleles. Read More

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January 2022

Molecular characterization of DNA gyrase and identification of mutations reduced susceptibility against quinolones .

Antimicrob Agents Chemother 2022 Jan 18:AAC0190221. Epub 2022 Jan 18.

Division of Bioresources, Hokkaido University International Institute for Zoonosis Control, Sapporo, Japan.

Buruli ulcer disease is a neglected necrotizing and disabling cutaneous tropical illness caused by (). Fluoroquinolone (FQ), used in the treatment of this disease, has been known to act by inhibiting the enzymatic activities of DNA gyrase; however, the detailed molecular basis of these characteristics and the FQ resistance mechanisms in remains unknown. This study investigated the detailed molecular mechanism of DNA gyrase and the contribution of FQ resistance using recombinant proteins from the subsp. Read More

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January 2022

«Development of an anti- biofilm phage cocktail: Genomic Adaptation to the Host».

Antimicrob Agents Chemother 2022 Jan 18:AAC0192321. Epub 2022 Jan 18.

Microbiology Department-Research Institute Biomedical A Coruña (INIBIC); Hospital A Coruña (CHUAC); University of A Coruña (UDC), A Coruña, Spain.

The need for alternatives to antibiotic therapy due to the emergence of multidrug resistant bacteria (MDR), such as the nosocomial pathogen , has led to the recovery of phage therapy. In addition, phages can be combined in cocktails to increase the host range. In this study, the evolutionary mechanism of adaptation was utilized in order to develop a phage adapted to , named phage Ab105-2phiΔCI404ad, from a mutant lytic phage, Ab105-2phiΔCI, previously developed by our group. Read More

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January 2022

Proteomic Correlates of Enhanced Daptomycin Activity Following β-Lactam Pre-Conditioning in Daptomycin-Resistant Methicillin-Resistant .

Antimicrob Agents Chemother 2022 Jan 18:AAC0201721. Epub 2022 Jan 18.

School of Pharmacy, University of Wisconsin-Madison, Madison, WI, 53705, USA.

Clinical treatment options for daptomycin (DAP)-resistant (DAP-R) methicillin-resistant (MRSA) infections are relatively limited. Current therapeutic strategies often take advantage of potential synergistic activity of DAP plus β-lactams; however, the mechanisms underlying their combinatorial efficacy are likely complex and remain incompletely understood. We recently showed that β-lactam passaging can re-sensitize DAP-R strains to a DAP-susceptible (DAP-S) phenotype. Read More

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January 2022

Interaction of Periplasmic Fab Production and Intracellular Redox Balance in Affects Product Yield.

ACS Synth Biol 2022 Jan 18. Epub 2022 Jan 18.

Christian Doppler Laboratory for Production of Next-Level Biopharmaceuticals in E. Coli, Department of Biotechnology, Institute of Bioprocess Science and Engineering, University of Natural Resources and Life Sciences, Vienna, Muthgasse 18, 1190 Vienna, Austria.

Antibody fragments such as Fab's require the formation of disulfide bonds to achieve a proper folding state. During their recombinant, periplasmic expression in , oxidative folding is mediated by the DsbA/DsbB system in concert with ubiquinone. Thereby, overexpression of Fab's is linked to the respiratory chain, which is not only immensely important for the cell's energy household but also known as a major source of reactive oxygen species. Read More

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January 2022

Plant growth-promoting properties of bacterial endophytes isolated from roots of L. and investigate their role as biofertilizers to enhance the essential oil contents.

Biomol Concepts 2021 Dec 31;12(1):175-196. Epub 2021 Dec 31.

Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11884, Egypt.

The main objective of the current study was to improve the essential oil contents of L. using bio-inoculation with bacterial endophytes. Therefore, out of fourteen endophytic bacterial isolates obtained from roots of , five isolates were selected based on the highest nitrogen-fixation and phosphate solubilization activity and identified as: T9r, T10r, T11r, T12r, and T13r. Read More

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December 2021

Implication of plant growth-promoting rhizobacteria of spp. as biocontrol agents against wilt disease caused by Schlecht. in L.

Biomol Concepts 2021 Dec 31;12(1):197-214. Epub 2021 Dec 31.

Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11884, Egypt.

Out of seven spp. isolated from infected faba bean roots, two were selected and showed faba bean-wilt disease severity with percentages of 68% and 47% under greenhouse conditions. The showed the highest wilt disease was selected to complete the current study. Read More

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December 2021

Corallococcus silvisoli sp. nov., a novel myxobacterium isolated from subtropical forest soil.

Arch Microbiol 2022 Jan 18;204(2):141. Epub 2022 Jan 18.

Key Laboratory of Agricultural Microbiomics and Precision Application - Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Guangdong Microbial Culture Collection Center (GDMCC), Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, China.

An orange-pigmented myxobacterium, designated strain c25j21, was isolated from subtropical forest soil collected from the Chebaling National Nature Reserve in Guangdong Province, China. Phylogenetic analysis based on the 16S rRNA gene and core genes clearly showed that the novel strain was affiliated within the genus Corallococcus and most closely related to Corallococcus aberystwythensis DSM 108846 (99.3% 16S rRNA gene sequence similarity), while C. Read More

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January 2022

Inhibition of sulfate-reducing bacteria with formate.

FEMS Microbiol Ecol 2022 Jan 18. Epub 2022 Jan 18.

University of Duisburg-Essen - Faculty of Chemistry - Environmental Microbiology and Biotechnology (EMB) - Aquatic Microbiology, Universitätsstr. 5, 45141 Essen, Germany.

Despite hostile environmental conditions, microbial communities have been found in µL-sized water droplets enclosed in heavy oil of the Pitch Lake, Trinidad. Some droplets showed high sulfate concentrations and surprisingly low relative abundances of sulfate-reducing bacteria in a previous study. Hence, we investigated here whether sulfate reduction might be inhibited naturally. Read More

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January 2022

Haemin deprivation renders Bacteroides fragilis hypersusceptible to metronidazole and cancels high-level metronidazole resistance.

J Antimicrob Chemother 2022 Jan 18. Epub 2022 Jan 18.

Institute for Specific Prophylaxis and Tropical Medicine Center for Pathophysiology, Infectiology, and Immunology, Medical University of Vienna, Kinderspitalgasse 15, A-1090 Vienna, Austria.

Background: Infections with Bacteroides fragilis are routinely treated with metronidazole, a 5-nitroimidazole antibiotic that is active against most anaerobic microorganisms. Metronidazole has remained a reliable treatment option, but resistance does occur, including in B. fragilis. Read More

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January 2022

Spread of hypervirulent multidrug-resistant ST147 Klebsiella pneumoniae in patients with severe COVID-19: an observational study from Italy, 2020-21.

J Antimicrob Chemother 2022 Jan 18. Epub 2022 Jan 18.

Infectious Disease Unit, Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy.

Objectives: To report an outbreak of hypervirulent Klebsiella pneumoniae (hvKp) in COVID-19 patients.

Methods: Prospective, observational study including consecutive COVID-19 patients with hvKp infections admitted to the University Hospital of Pisa (Italy). Clinical data and outcome of patients were collected. Read More

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January 2022

Transmission barrier of the blaKPC plasmid mediated by type I restriction-modification systems in Escherichia coli.

J Antimicrob Chemother 2022 Jan 18. Epub 2022 Jan 18.

Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, People's Republic of China.

Background: Transportation of carbapenem-resistant plasmids contributes to carbapenem resistance in Gram-negative bacteria. KPC enzymes are the most clinically important enzymes among carbapenem-resistant Klebsiella pneumoniae, whereas the rate of blaKPC in Escherichia coli is low. The CRISPR-Cas system and restriction-modification system (R-M system) in bacteria defend against invading genomes. Read More

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January 2022

Fine-Scale Reconstruction of the Evolution of FII-33 Multidrug Resistance Plasmids Enables High-Resolution Genomic Surveillance.

mSystems 2022 Jan 18:e0083121. Epub 2022 Jan 18.

Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China.

We examined 185 complete, publicly available FII-33 plasmid sequences, characterizing their backbone and various insertions. The variable characteristic insertions facilitated evolutionary reconstruction for this plasmid group, beginning with the acquisition of a primary resistance region (PRR) over 10 years ago. FII-33 plasmids have evolved by acquiring additional resistance genes in the PRR via translocatable elements and by forming cointegrates with plasmids of other types. Read More

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January 2022