16 results match your criteria strain hisb

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HisB as novel selection marker for gene targeting approaches in Aspergillus niger.

BMC Microbiol 2017 03 8;17(1):57. Epub 2017 Mar 8.

Institute of Biotechnology, Department of Applied and Molecular Microbiology, Technische Universität Berlin, Gustav-Meyer-Allee 25, 13355, Berlin, Germany.

Background: For Aspergillus niger, a broad set of auxotrophic and dominant resistance markers is available. However, only few offer targeted modification of a gene of interest into or at a genomic locus of choice, which hampers functional genomics studies. We thus aimed to extend the available set by generating a histidine auxotrophic strain with a characterized hisB locus for targeted gene integration and deletion in A. Read More

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Genetic manipulations of the hyperthermophilic piezophilic archaeon Thermococcus barophilus.

Appl Environ Microbiol 2014 Apr 31;80(7):2299-306. Epub 2014 Jan 31.

Université de Bretagne Occidentale, UMR 6197-Laboratoire de Microbiologie des Environnements Extrêmes, Institut Universitaire Européen de la Mer, Plouzané, France.

In this study, we developed a gene disruption system for Thermococcus barophilus using simvastatin for positive selection and 5-fluoroorotic acid (5-FOA) for negative selection or counterselection to obtain markerless deletion mutants using single- and double-crossover events. Disruption plasmids carrying flanking regions of each targeted gene were constructed and introduced by transformation into wild-type T. barophilus MP cells. Read More

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[Relationship of histidine auxotrophy of Acidovorax citrulli with pathogenicity].

Wei Sheng Wu Xue Bao 2011 Sep;51(9):1185-93

College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, China.

Unlabelled: Acidovorax citrulli (Ac) is an important bacterium that occurs in watermelon, melon and other cucurbits. It mainly damages watermelon and melon, and can cause leaf blight, fruit rot, and even mortality.

Objective: To verify the relationship between defects in the synthesis of histidine and the pathogenicity of Ac. Read More

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September 2011

[Expression and antigenicity analysis of NTPase gene of Toxoplasma gondii].

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 2007 Dec;25(6):447-50

Wenzhou Medical College, Wenzhou 325035, China.

Objective: To clone and express prokaryotic recombinant plasmid of nucleoside triphosphate hydrolase (NTPase) gene of Toxoplasma gondii, and analyze its antigenicity.

Method: NTPase gene was amplified by PCR from RH strain of T. gondii and cloned into pGEM-T Easy vector. Read More

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December 2007

Stable expression of Hantavirus H8205 strain G1/IL-2 gene and immune protection of the fusion gene.

J Huazhong Univ Sci Technolog Med Sci 2007 Apr;27(2):124-7

Department of Pathogenetic Biology, School of Basic Medical Science, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

To explore the feasibility of stable expression of Hantavirus H8205 strain G1 segment and human IL-2 fusion gene in Vero cells, and to examine the immune protection effects on mice vaccinated with this recombinant eukaryotic expression vector containing Hantavirus G1 gene and IL-2 gene. With the help of lipofectamine, the Vero cells were transfected with pcDNA3.1/HisB-IL-2-G1 and the positive cells were selected by G418. Read More

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gmhX, a novel gene required for the incorporation of L-glycero-D-manno-heptose into lipooligosaccharide in Neisseria meningitidis.

Microbiology (Reading) 2001 Aug;147(Pt 8):2367-2377

Department of Veterans Affairs Medical Center, Atlanta, GA 30033, USA2.

Lipooligosaccharide (LOS) is a critical virulence factor of Neisseria meningitidis. A Tn916 insertion mutant, designated 469, was found to exhibit a markedly truncated LOS of 2.9 kDa when compared by Tricine/SDS-PAGE to the parental LOS (4. Read More

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Regulation of the Aspergillus nidulans hisB gene by histidine starvation.

Curr Genet 2001 Jan;38(6):314-22

Institut für Mikrobiologie und Genetik, Georg-August-Universität, Göttingen, Germany.

The hisB gene of the filamentous fungus Aspergillus nidulans encodes imidazole glycerol-phosphate dehydratase (E.C. 4. Read More

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January 2001

Anabaena sp. strain PCC 7120 bifA gene encoding a sequence-specific DNA-binding protein cloned by in vivo transcriptional interference selection.

J Bacteriol 1993 Jul;175(13):4025-35

Department of Biology, Texas A&M University, College Station 77843-3258.

VF1 is a DNA-binding protein from the cyanobacterium Anabaena sp. strain PCC 7120. VF1 was originally identified on the basis of its binding affinity to the upstream region of xisA, which encodes a heterocyst-specific site-specific recombinase. Read More

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Gene inactivation in Lactococcus lactis: histidine biosynthesis.

J Bacteriol 1993 Jul;175(14):4391-9

Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France.

Lactococcus lactis strains from dairy and nondairy sources were tested for the ability to grow in the absence of histidine. Among 60 dairy strains tested, 56 required histidine, whereas only 1 of 11 nondairy strains had this requirement. Moreover, 10 of the 56 auxotrophic strains were able to grow in the presence of histidinol (Hol+), the immediate histidine precursor. Read More

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[Cloning and inactivation of a chromosomal copy of the imidazole glycerophosphate dehydratase (HIS) gene from Hansenula polymorpha].

Mol Gen Mikrobiol Virusol 1991 Jul(7):25-8

A gene for imidazole glycerophosphate dehydratase (HIS) has been selected from the library of Hansenula polymorpha genes by complementation of Escherichia coli hisB mutations or his3 mutation of Saccharomyces cerevisiae. Inactivation of the gene in Hansenula polymorpha strain 48V, as well as Leu(-)-Leu+ conversion of phenotype and arising of antibiotic G418 resistance resulted from transformation of the strain by the linear DNA molecules of the cloned HIS-gene with the in vitro inserted LEU2 gene from Saccharomices cerevisiae and KmR gene. The Southern hybridization analysis of the Leuf+His- G418R clones representing 1-2% of transformants population together with the DNA analysis of the monospore clones from the hybrid Leu+His-G418R transformant tetrad and tester strain have revealed the locus specific nature of the clones. Read More

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In vivo synthesis of histidine by a cloned histidine ammonia-lyase in Escherichia coli.

Authors:
R L Fuchs J F Kane

J Bacteriol 1985 Apr;162(1):98-101

Histidine ammonia-lyase catalyzes the first step in histidine catabolism, the deamination of histidine to urocanate and ammonia. In vitro experiments have shown that histidine ammonia-lyase also can catalyze the reverse (amination) reaction, histidine synthesis, relatively efficiently under extreme reaction conditions (4 M NH4OH, pH 10). An Escherichia coli hisB deletion strain was transformed with a pBR322 derivative plasmid (pCB101) containing the entire Klebsiella aerogenes histidine utilization (hut) operon to determine whether the catabolic histidine ammonia-lyase could function biosynthetically in vivo to satisfy the histidine auxotrophy. Read More

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Pleiotropic effect of his gene mutations on nitrogen fixation in Klebsiella pneumoniae.

EMBO J 1982 ;1(2):197-204

ARC Unit of Nitrogen Fixation, University of Sussex, Brighton, BN1 9RQ, UK.

Several his mutations were found to influence nitrogen fixation in Klebsiella pneumoniae: hisB, hisC, and hisD mutants had 50% of wild-type levels of nitrogenase activity when supplied with 30 mug or less histidine/ml although this concentration did not limit protein synthesis and the mutants retained a Nif plate phenotype. A hisA mutation had a similar but more dramatic effect. At low concentrations of histidine the hisA mutant strain had only 5% of the nitrogenase activity found at high histidine concentration or in a his strain, and was also Nif on low histidine agar plates. Read More

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Properties of the imidazolylacetolphosphate aminotransferase produced in a mutant demonstrating no apparent genetic involvement of the structural gene.

J Bacteriol 1975 Jul;123(1):233-41

Genetic studies with strain hisBH22 of Salmonella typhimurium indicate it contains a deletion within the histidine operon involving part of the hisH gene and all of the hisB gene, but not extending into the adjacent hisC gene which is adjacent to hisB. However, the specific activity of the hisC product, imidazolylacetolphosphate aminotransferase (EC 2.6. Read More

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A genetic and biochemical study of histidine biosynthesis in Micrococcus luteus.

Genetics 1975 Mar;79(3):361-76

Histidine auxotrophs of Micrococcus luteus strain ATCC 27141 were induced by treatment of the parent strain with N-methyl-N'-nitro-N-nitrosoguanidine. Auxotrophs were biochemically characterized by examining culture accumulations of histidine intermediates, using paper chromatography and the Bratton-Marshall test, and growth responses to L-histidinol. his(IG) mutants failed to accumulate Pauly-positive imidazoles; his(EAHF) mutants accumulated 5-amino-1-ribosyn-4-imidazole carboxamide; hisB mutants accumulated imidazoleglycerol; hisC mutants accumulated imidazoleacetol; hisD mutants, but did stimulate all other histidine mutants, blocked at earlier steps in the biosynthetic pathway. Read More

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Evidence for a composite state of an F'his, gnd element and a cryptic plasmid in a derivative of Salmonella typhimurium LT2.

J Bacteriol 1974 Jul;119(1):192-201

A method designed to select mutants constitutive for expression of the histidine operon has been applied to a Salmonella typhimurium LT2 strain containing an F'his,gnd element and a cryptic plasmid. One of the mutants isolated, strain AA0019, has not only increased levels of histidinol phosphate phosphatase (hisB), but also increased levels of gluconate-6-phosphate dehydrogenase (gnd). Ultracentrifugation studies of extrachromosomal deoxyribonucleic acid (DNA) isolated from strain AA0019 revealed the presence of a single species of covalently closed circular (CCC) DNA that sedimented more rapidly through neutral and alkaline sucrose gradients than any of its possible plasmid precursors. Read More

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Evidence for proteolytic degradation of histidinol phosphate phosphatase specified by nonsense mutants of the hisB gene of Salmonella typhimurium.

Authors:
L L Houston

J Bacteriol 1973 Oct;116(1):88-97

The gene products from hisB nonsense mutants having histidinol phosphate phosphatase activity were isolated from Salmonella typhimurium. The enzyme from strain TR691 (hisB278 hisT1529 aroD5) was isolated in the presence of diisopropylfluorophosphate. Three electrophoretically separable forms were demonstrated, and all were shown to have a mol wt of approximately 38,000 and to consist of a single polypeptide chain. Read More

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October 1973
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