475,839 results match your criteria strain

Synergy between indigenous bacteria and extracellular electron shuttles enhances transformation and mobilization of Fe(III)/As(V).

Sci Total Environ 2021 Apr 9;783:147002. Epub 2021 Apr 9.

School of Environmental Studies, China University of Geosciences, Wuhan 430074, PR China.

The reduction of Fe(III) by metal-reducing bacteria through extracellular electron transfer (EET) is a critical link in the biogeochemical cycle of As/Fe, and humic substances are believed to play a role in this process. In this study, the indigenous As-resistant bacterium Bacillus D2201 isolated from the Datong Basin was responsible for the valence transition of Fe and As in the groundwater environment. The bacterium has both the arsC gene for intracellular arsenate reduction and an EET pathway for transferring electrons to an electrode or Fe(III). Read More

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Remodelling of trabecular bone in human distal tibia: A model based on an in-vivo HR-pQCT study.

J Mech Behav Biomed Mater 2021 Apr 14;119:104506. Epub 2021 Apr 14.

Wolfson School of Mechanical, Electrical and Manufacturing Engineering, Loughborough University, Loughborough, UK.

An abnormal remodelling process of bones can lead to various bone disorders, such as osteoporosis, making them prone to fracture. Simulations of load-induced remodelling of trabecular bone were used to investigate its response to mechanical signal. However, the role of mechanostat in trabecular-bone remodelling has not yet been investigated in simulations underpinned by a longitudinal in-vivo study in humans. Read More

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Mechanical characterization of abdominal aortas using multi-perspective ultrasound imaging.

J Mech Behav Biomed Mater 2021 Apr 14;119:104509. Epub 2021 Apr 14.

Photoacoustics & Ultrasound Laboratory Eindhoven (PULS/e), Department of Biomedical Engineering, Eindhoven University of Technology, P.O. Box 513, 5600 MB, Eindhoven, the Netherlands.

Mechanical characterization of abdominal aortic aneurysms using personalized biomechanical models is being widely investigated as an alternative criterion to assess risk of rupture. These methods rely on accurate wall motion detection and appropriate model boundary conditions. In this study, multi-perspective ultrasound is combined with finite element models to perform mechanical characterization of abdominal aortas in volunteers. Read More

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Risk factors and mechanisms of in vivo emergence of colistin resistance in carbapenem-resistant Klebsiella pneumoniae.

Int J Antimicrob Agents 2021 Apr 14:106342. Epub 2021 Apr 14.

Division of Infectious Diseases, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan; Institute of Emergency and Critical Care Medicine, National Yang Ming Chiao Tung University, Hsinchu, Taiwan. Electronic address:

Colistin is one of the last-resort antibiotics for treating carbapenem-resistant Klebsiella pneumoniae (CRKP). However, colistin resistance in CRKP poses a global antimicrobial crisis as therapeutic options are limited. We investigated risk factors for in vivo emergence of colistin resistance in CRKP and explored the underlying resistance mechanisms. Read More

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The transcriptional regulator GON4L is required for viability and hematopoiesis in mice.

Exp Hematol 2021 Apr 14. Epub 2021 Apr 14.

Departments of Internal MedicineCarver College of Medicine, University of Iowa, 375 Newton Road, Iowa City, IA, 52242, USA; Anatomy and Cell BiologyCarver College of Medicine, University of Iowa, 375 Newton Road, Iowa City, IA, 52242, USA; Interdisciplinary Graduate Program in Immunology, Carver College of Medicine, University of Iowa, 375 Newton Road, Iowa City, IA, 52242, USA. Electronic address:

The Gon4l gene encodes a putative transcriptional regulator implicated in the control of both cell differentiation and proliferation. Previously, we described a mutant mouse strain called Justy in which splicing of pre-mRNA generated from Gon4l is disrupted. This defect severely reduces, but does not abolish, GON4L protein expression, and blocks the formation of early B-lineage progenitors, suggesting Gon4l is required for B cell development in vertebrates. Read More

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Autochthonous and imported tegumentary leishmaniasis in Catalonia (Spain): aetiological evolution in the last four decades and usefulness of different typing approaches based on biochemical, molecular and proteomic markers.

Transbound Emerg Dis 2021 Apr 17. Epub 2021 Apr 17.

Secció de Parasitologia, Departament de Biologia, Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona, Sanitat i Medi Ambient, Barcelona, Spain.

Leishmaniasis is a transmissible disease caused by Leishmania protozoa. Spain is endemic for both visceral and cutaneous leishmaniasis, the autochthonous aetiological agent being Leishmania infantum. Around the world, the L. Read More

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Nocardioides luti sp. nov., belonging to the family Nocardioidaceae isolated from kaolinite, exhibiting the biosynthesis potential of alkylresorcinol.

Antonie Van Leeuwenhoek 2021 Apr 17. Epub 2021 Apr 17.

Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National University, Incheon, 22012, Republic of Korea.

A novel Gram-staining-positive, short rod-shaped, non-motile, and non-pigmented actinobacterial strain (KIGAM211) was isolated from kaolinite, a soft white clay mineral, collected from Sancheong in the Republic of Korea. On the basis of 16S rRNA gene sequence analysis, strain KIGAM211 was determined to belong to the genus Nocardioides and was most closely related to N. ungokensis UKS-03 (97. Read More

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Halobellus ruber sp. nov., a deep red-pigmented extremely halophilic archaeon isolated from a Korean solar saltern.

Antonie Van Leeuwenhoek 2021 Apr 17. Epub 2021 Apr 17.

Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National University, Incheon, 22012, Republic of Korea.

A novel halophilic archaeon, strain MBLA0160, was isolated from a solar saltern in Sorae, Republic of Korea. The cells are deep-red pigmented, Gram-negative, rod shaped, motile, and lysed in distilled water. The strain MBLA0160 grew at 25-45 °C (optimum 37 °C), in 15-30% (w/v) NaCl (optimum 20%) and 0. Read More

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Vibrio nitrifigilis sp. nov., a marine nitrogen-fixing bacterium isolated from the lagoon sediment of an islet inside an atoll.

Antonie Van Leeuwenhoek 2021 Apr 17. Epub 2021 Apr 17.

Institute of Oceanography, National Taiwan University, P.O. Box 23-13, Taipei, 10617, Taiwan.

A nitrogen-fixing isolate of facultatively anaerobic, marine bacterium, designated strain NFV-1, was recovered from the lagoon sediment of Dongsha Island, Taiwan. It was a Gram-negative rod which exhibited motility with monotrichous flagellation in broth cultures. The strain required NaCl for growth and grew optimally at about 25-35 °C, 3% NaCl and pH 7-8. Read More

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Marinifaba aquimaris gen. nov., sp. nov., a novel chitin-degrading gammaproteobacterium in the family Alteromonadaceae isolated from seawater of the Mariana Trench.

Antonie Van Leeuwenhoek 2021 Apr 17. Epub 2021 Apr 17.

State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao, 266237, China.

A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium, designated strain SM1970, was isolated from a seawater sample collected from the Mariana Trench. Strain SM1970 grew at 15-37 C and with 1-5% (w/v) NaCl. It hydrolyzed colloidal chitin, agar and casein but did not reduce nitrate to nitrite. Read More

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Massilia aromaticivorans sp. nov., a  BTEX Degrading Bacterium Isolated from Arctic Soil.

Curr Microbiol 2021 Apr 17. Epub 2021 Apr 17.

Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Republic of Korea.

A novel BTEX degrading bacterial strain, designated ML15P13, was isolated from Arctic soil at the Svalbard Islands, Norway, using an enrichment culture technique. This isolate is Gram-negative, aerobic, motile with multiple flagella at one polar end, and rod-shaped. Growth was observed at 4-35 °C, pH 6. Read More

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Improving the design of an oxidative stress sensing biosensor in yeast.

FEMS Yeast Res 2021 Apr 17. Epub 2021 Apr 17.

Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, M5S 1A1, Canada.

Transcription factor (TF)-based biosensors have proven useful for increasing biomanufacturing yields, large-scale functional screening, and in environmental monitoring. Most yeast TF-based biosensors are built from natural promoters, resulting in large DNA parts retaining considerable homology to the host genome, which can complicate biological engineering efforts. There is a need to explore smaller, synthetic biosensors to expand the options for regulating gene expression in yeast. Read More

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A probabilistic approach for modelling bone fracture healing under Ilizarov circular fixator.

Int J Numer Method Biomed Eng 2021 Apr 17:e3466. Epub 2021 Apr 17.

Department of Infrastructure Engineering, The University of Melbourne, Parkville, Victoria, Australia.

Bone fracture treatments using Ilizarov circular fixator (ICF) involve dealing with uncertainties about a range of critical factors that control the mechanical microenvironment of the fracture site such as ICF configuration, fracture gap size, physiological loading etc. To date, the effects of the uncertainties about these critical factors on the mechanical microenvironment of the fracture site have not been fully understood. The purpose of this study is to tackle this challenge by using computational modelling in conjunction with engineering reliability analysis. Read More

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Masked and Distanced: A Qualitative Study of How Personal Protective Equipment and Distancing Affect Teamwork in Emergency Care.

Int J Qual Health Care 2021 Apr 17. Epub 2021 Apr 17.

Department of Health Policy and Management, Harvard T.H. Chan School of Public Health, MA.

Background: Newly intensified use of personal protective equipment (PPE) in emergency departments presents teamwork challenges affecting the quality and safety of care at the frontlines. We conducted a qualitative study to categorize and describe barriers to teamwork posed by PPE and distancing in the emergency setting.

Methods: We conducted 55 semi-structured interviews between June-August, 2020 with personnel from two emergency departments serving in a variety of roles. Read More

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Site-specific features of active muscle stiffness and proximal aponeurosis strain in biceps femoris long head.

Scand J Med Sci Sports 2021 Apr 16. Epub 2021 Apr 16.

Graduate School of Engineering and Science, Shibaura Institute of Technology, Saitama, Japan.

Limited information is available on site-specific features of muscle stiffness and aponeurosis strain of the biceps femoris long head (BFlh) during contractions. Therefore, understanding of the mechanics and etiology of hamstring strain injuries remains difficult. As a first step to gain further insight into them, the present study aimed to identify whether active muscle stiffness and proximal aponeurosis strain during contractions are varied along the long-axis of the BFlh. Read More

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A Clinical and Biologic Review of Keratoacanthoma.

Br J Dermatol 2021 Apr 16. Epub 2021 Apr 16.

Department of Dermatology, Henry Ford Health System, Detroit, MI, USA.

Keratoacanthoma (KA) is a common skin tumor that remains controversial regarding classification, epidemiology, diagnosis, prognosis, and management. Classically, a KA manifests as a rapidly growing, well-differentiated, squamoid lesion with a predilection for sun-exposed sites in the elderly and a tendency to spontaneously regress. Historically, KAs have been considered a variant of cutaneous squamous cell carcinoma (cSCC) and are often reported as KA-type cSCC. Read More

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Tunable characteristics of low-frequency bandgaps in two-dimensional multivibrator phononic crystal plates under prestrain.

Sci Rep 2021 Apr 16;11(1):8389. Epub 2021 Apr 16.

School of Mathematics and Physics, Lanzhou Jiaotong University, Lanzhou, 730070, China.

In view of the influence of variability of low-frequency noise frequency on noise prevention in real life, we present a novel two-dimensional tunable phononic crystal plate which is consisted of lead columns deposited in a silicone rubber plate with periodic holes and calculate its bandgap characteristics by finite element method. The low-frequency bandgap mechanism of the designed model is discussed simultaneously. Accordingly, the influence of geometric parameters of the phononic crystal plate on the bandgap characteristics is analyzed and the bandgap adjustability under prestretch strain is further studied. Read More

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Microscale sensor solution for data collection from fibre-matrix interfaces.

Sci Rep 2021 Apr 16;11(1):8346. Epub 2021 Apr 16.

Faculty of Engineering and Natural Sciences, Tampere University, PO Box 589, 33014, Tampere, Finland.

Especially the applications of fibrous composites in miniature products, dental and other medical applications require accurate data of microscale mechanics. The characterization of adhesion between single filament and picoliter-scale polymer matrix usually relies on the experiments using so-called microbond (MB) testing. The traditional MB test systems provide unitary data output (i. Read More

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Research approaches for evaluating opioid sparing in clinical trials of acute and chronic pain treatments: IMMPACT recommendations.

Pain 2021 Apr 8. Epub 2021 Apr 8.

University of Rochester Medical Center, Rochester, NY, USA University of Washington, Seattle, WA, USA Stony Brook School of Medicine, Stony Brook, NY, USA Queens University, Kingston, Ontario, Canada (Formally) U.S. Food and Drug Administration, Silver Spring, MD, USA WCG Analgesic Solutions, Wayland, MA, USA John's Hopkins School of Medicine, Baltimore, MD, USA University of California San Francisco, San Francisco, CA, USA University of Pennsylvania, Philadelphia, PA, USA Indiana University School of Medicine, Indianapolis, IN, USA Brigham and Women's Hospital / Harvard Medical School, Boston, MA, USA Carolinas Pain Institute, Charlotte, NC, USA Innocoll, Athlone, Ireland American Chronic Pain Association, Rocklin, CA, USA Wake Forest School of Medicine, Winston-Salem, NC, USA Southern Illinois University Edwardsville, Edwardsville, IL, USA GW Pharmaceuticals, Carlsbad, CA, USA Biogen, Cambridge, MA, USA University at Buffalo, Buffalo, NY, USA McGill University, Montreal, Canada MCPHS University, Boston, MA National Institutes of Health, Bethesda, MD, USA U.S. Department of Veterans Affairs / George Washington University, Washington, DC, USA (Formally) Pacira , Parsippany, NY, USA SDG LLC, Cambridge, MA, USA Sarepta, Cambridge, MA, USA Pfizer, NYC, NY, USA University of Pittsburgh, Pittsburgh, PA, USA Flexion Therapeutics, Burlington, MA, USA.

Abstract: Randomized clinical trials have demonstrated the efficacy of opioid analgesics for the treatment of acute and chronic pain conditions, and for some patients, these medications may be the only effective treatment available. Unfortunately, opioid analgesics are also associated with major risks (e.g. Read More

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Update in imaging of cancer therapy-related cardiac toxicity in adults.

Open Heart 2021 Apr;8(1)

Pediatric Cardiology, Wilhelmina Children's Hospital University Medical Centre, Utrecht, The Netherlands

Over the past decades, prognosis of patients with cancer has strongly improved and the number of cancer survivors is rapidly growing. Despite this success, cancer treatment is associated with development of serious cardiovascular diseases including left ventricular (LV) systolic dysfunction, heart failure, valvular disease, myocardial infarction, arrhythmias or pericardial diseases. Serial non-invasive cardiac imaging is an important tool to detect early signs of cardiotoxicity, to allow for timely intervention and provide optimal circumstances for long-term prognosis. Read More

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Pyruvate production by using pyruvate dehydrogenase variants.

Appl Environ Microbiol 2021 Apr 16. Epub 2021 Apr 16.

Department of Microbiology, University of Georgia, Athens, GA, 30602, USA

Altering metabolic flux at a key branchpoint in metabolism has commonly been accomplished through gene knockouts or by modulating gene expression. An alternative approach to direct metabolic flux preferentially toward a product is decreasing the activity of a key enzyme through protein engineering. In , pyruvate can accumulate from glucose when carbon flux through the pyruvate dehydrogenase complex is suppressed. Read More

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Molecular dissection of perithecial mating line development in , a species with a nontypical mating system featured by plus-to-minus switch and plus-minus mediated sexual enhancement.

Appl Environ Microbiol 2021 Apr 16. Epub 2021 Apr 16.

State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi Province, 712100, China.

The genetic regulation of (Glomerella) sexual reproduction does not strictly adhere to the Ascomycota paradigm and remains poorly understood. Morphologically different but sexually compatible strain types termed 'plus' and 'minus' have been recognized but the biological and molecular distinctions between these strain types remain elusive. In this study, we characterized the sexual behaviors of a pair of plus and minus strains of with the aid of live cell nuclear-localized fluorescent protein labeling, gene expression and gene mutation analyses. Read More

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Construction of a fimbriae absent by deleting 64 genes and its application for efficient production of poly-3-hydroxybutyrate and L-threonine.

Appl Environ Microbiol 2021 Apr 16. Epub 2021 Apr 16.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu province, China;

contains 12 chaperone-usher operons for biosynthesis and assembly of various fimbriae. In this study, each of the 12 operons was deleted in MG1655, and the resulting 12 deletion mutants all grew better than the wild type, especially in the nutrient-deficient M9 medium. When the plasmid pBHR68 containing the key genes for polyhydroxyalkanoate production was introduced into these 12 mutants, each mutant synthesized more polyhydroxyalkanoate than the wild type control. Read More

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Symbiont genomic features and localization in the bean beetle, .

Appl Environ Microbiol 2021 Apr 16. Epub 2021 Apr 16.

Department of Biology, Emory University, 30322, Atlanta, GA, USA

A pervasive pest of stored leguminous products, the bean beetle (Coleoptera: Chrysomelidae: ) associates with a simple bacterial community during adulthood. Despite its economic importance, little is known about the compositional stability, heritability, localization, and metabolic potential of the bacterial symbionts of In this study, we applied community profiling using 16S rRNA gene sequencing to reveal a highly conserved bacterial assembly shared between larvae and adults. Dominated by and , this community is localized extracellularly along the epithelial lining of the bean beetle's digestive tract. Read More

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Comparison of two mathematical models for predicted human thermal responses to hot and humid environments.

J Therm Biol 2021 Apr 11;97:102902. Epub 2021 Mar 11.

LDM Associates, San Jose, CA, USA. Electronic address:

Purpose: We compared the accuracy and design of two thermoregulatory models, the US Army's empirically designed Heat Strain Decision Aid (HSDA) and the rationally based Health Risk Prediction (HRP) for predicting human thermal responses during exercise in hot and humid conditions and wearing chemical protective clothing.

Methods: Accuracy of the HSDA and HRP model predictions of core body and skin temperature (Tc, Ts) were compared to each other and relative to measured outcomes from eight male volunteers (age 24 ± 6 years; height 178 ± 5 cm; body mass 76.6 ± 8. Read More

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Short-term hot water immersion results in substantial thermal strain and partial heat acclimation; comparisons with heat-exercise exposures.

J Therm Biol 2021 Apr 25;97:102898. Epub 2021 Feb 25.

Department of Kinesiology, California Baptist University, 8432 Magnolia Ave, Riverside, CA, 92504, USA. Electronic address:

Objective: To examine the effectiveness of hot water immersion (HWI) as a heat acclimation strategy in comparison to time and temperature matched, exercise-heat acclimation (EHA).

Methods: 8 males performed heat stress tests (HST) (45 min of cycling at 50% of VO in 40 °C, 40% RH) before and after heat acclimation sessions. Acclimation sessions were either three consecutive bouts of HWI (40 min of submersion at 40 °C) or EHA (40 min of cycling at 50% VO in 40 °C, 40% RH). Read More

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Core temperature circadian rhythm across aging in Spontaneously Hypertensive Rats.

J Therm Biol 2021 Apr 23;97:102807. Epub 2021 Feb 23.

Laboratório de Biologia do Exercício, Departamento de Educação Física, Universidade Federal de Viçosa, Viçosa, MG, Brazil. Electronic address:

The purpose of this study was to evaluate the circadian rhythm of core temperature (T) across aging in Spontaneously Hypertensive Rats (SHR) with comparison to the two rat strains often used as their normotensive control animals, namely, Wistar (WIS) and Wistar Kyoto (WKY).

Methods: WIS, WKY and SHR rats were subdivided into three different groups according their age: WIS16, WIS48, WIS72, WKY16, WKY48, WKY72, SHR16, SHR48 and SHR72 weeks-old. Body mass and blood pressure were periodically measured along the experiments. Read More

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Stability of BSE infectivity towards heat treatment even after proteolytic removal of prion protein.

Vet Res 2021 Apr 16;52(1):59. Epub 2021 Apr 16.

Department of Poultry Science, North Carolina State University, Raleigh, NC, 27695-7608, USA.

The unconventional infectious agents of transmissible spongiform encephalopathies (TSEs) are prions. Their infectivity co-appears with PrP, aberrant depositions of the host's cellular prion protein (PrP). Successive heat treatment in the presence of detergent and proteolysis by a keratinase from Bacillus licheniformis PWD-1 was shown before to destroy PrP from bovine TSE (BSE) and sheep scrapie diseased brain, however data regarding expected reduction of infectivity were still lacking. Read More

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Genome sequencing and comparative genomic analysis of highly and weakly aggressive strains of Sclerotium rolfsii, the causal agent of peanut stem rot.

BMC Genomics 2021 Apr 16;22(1):276. Epub 2021 Apr 16.

Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, P.R. China, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, 430062, China.

Background: Stem rot caused by Sclerotium rolfsii is a very important soil-borne disease of peanut. S. rolfsii is a necrotrophic plant pathogenic fungus with an extensive host range and worldwide distribution. Read More

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Methylene blue photochemical treatment as a reliable SARS-CoV-2 plasma virus inactivation method for blood safety and convalescent plasma therapy for COVID-19.

BMC Infect Dis 2021 Apr 16;21(1):357. Epub 2021 Apr 16.

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Disease, National Medical Center for Infectious Disease, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University , Hangzhou, 310003, China.

Background: In 2020, a new coronavirus, SARS-CoV-2, quickly spread worldwide within a few months. Although coronaviruses typically infect the upper or lower respiratory tract, the virus RNA can be detected in plasma. The risk of transmitting coronavirus via transfusion of blood products remains. Read More

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