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Restriction of Iron Loading into Developing Seeds by A YABBY Transcription Factor Safeguards Successful Reproduction in Arabidopsis.

Mol Plant 2021 Jun 8. Epub 2021 Jun 8.

State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China;. Electronic address:

Iron (Fe) storage in plant seeds is not only necessary for seedling establishment following germination, but also a major source of dietary Fe for human and animal life. Seed Fe accumulation was known to be low during early seed development. However, the underlying mechanism and biological significance remain elusive. Read More

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UDP-GlcNAc-1-phosphotransferase is a clinically important regulator of human and mouse hair pigmentation.

J Invest Dermatol 2021 Jun 8. Epub 2021 Jun 8.

Centre for Dermatology Research, University of Manchester, UK; The NIHR Biomedical Research Centre, Manchester, UK;; Dr Phillip Frost Department of Dermatology and Cutaneous Surgery, University of Miami Miller School of Medicine, Miami, FL, USA;; Monasterium Laboratory, Münster, Germany. Electronic address:

UDP-GlcNAc-1-phosphotransferase, a product of two separate genes (GNPTAB, GNPTG), is essential for sorting and transport of lysosomal enzymes to lysosomes. GNPTAB gene defects cause extracellular missorting of lysosomal enzymes resulting in lysosomal storage diseases, namely mucolipidosis (ML) type II, which is associated with hair discoloration. Yet, the physiological functions of GNPTAB in the control of hair follicle (HF) pigmentation remain unknown. Read More

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Wet vs. dry inoculation methods have a significant effect of Listeria monocytogenes growth on many types of whole intact fresh produce.

J Food Prot 2021 Jun 11. Epub 2021 Jun 11.

Rutgers University Food Science 65 Dudley Road UNITED STATES New Brunswick NJ 08901 732-407-7729.

L. monocytogenes causes relatively few outbreaks linked to whole fresh produce but triggers recalls each year in the US. There are limited data on the influence of wet vs. Read More

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Effect of organic loading on phosphorus forms transformation and microbial community in continuous-flow A/O process.

Water Sci Technol 2021 Jun;83(11):2640-2651

Tianjin IKWEN Water Treatment Co., Ltd, Tianjin 300000, China.

A continuous-flow Anaerobic/Anoxic/Oxic (A/O) system was operated at different organic concentrations to systematically investigate the effect on the nutrient removal, secretion characteristics of extracellular polymer, phosphorus forms transformation and changes in functional flora in this system. The results showed that high organic loading was more conducive to promote the secretion of extracellular polymeric substance (EPS), the increase of polysaccharide content was more obvious compared with protein, the impact of organic loading on the components of loosely bound EPS (LB-EPS) was higher than that of tight-bound EPS (TB-EPS). Phosphorus in sludge floc mainly existed in the form of inorganic phosphorus (IP), and IP mainly existed in the form of apatite inorganic phosphorus (AP). Read More

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Feasibility assessment of bentonite drilling mud to improve the physical quality of loamy sand soil and water deficit of forest plant seedlings.

J Air Waste Manag Assoc 2021 Jun 11. Epub 2021 Jun 11.

Project Environmental Management Department, PTT Public Company Limited, Bangkok-10900, Thailand.

Spent drilling mud (DM), a co-product during horizontal directional drilling operations, needs proper disposal to comply with social and environmental sustainability awareness, with one option being its cost-effective reuse as a soil amendment. The possibility was investigated of DM applications to improve the physical quality of loamy sand soil. The soil was treated with increasing DM contents in a laboratory at volumetric DM-to-soil ratios of 0:100 (DM0, no DM), 25:75 (DM25), 50:50 (DM50), 75:25 (DM75), and 100:0 (DM100, no soil). Read More

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Human monocytes store and secrete preformed CCL5, independent of de novo protein synthesis.

J Leukoc Biol 2021 Jun 11. Epub 2021 Jun 11.

Department of Biological Sciences, University of Toronto Scarborough, Toronto, Ontario, Canada.

Monocytes are a subset of circulating peripheral blood mononuclear cells with diverse roles in immunity, including sentinel roles in cytokine secretion. Conventionally, cytokines require an inductive stimulus for their expression and secretion, resulting in a time lag from the time of stimulation to when the proteins are packaged and secreted. Because cytokines are the main communicators in the immune system, their temporal expression is a key factor in coordinating responses to efficiently resolve infection. Read More

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Tfcancer: a manually curated database of transcription factors associated with human cancers.

Bioinformatics 2021 May 26. Epub 2021 May 26.

School of Life Sciences and Engineering, Southwest Jiaotong University, Chengdu 610031, China.

Summary: Transcription factors (TFs) are critical regulation elements and its dysregulation can lead to a variety of cancers. However, currently, there are no such online resources for large-scale collection, storage and analysis of TF-cancer associations in those cancers. To fill this gap, we present a database called TFcancer (http://lcbb. Read More

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Trigger finger in children with hurler syndrome - distribution pattern and treatment options.

GMS Interdiscip Plast Reconstr Surg DGPW 2021 5;10:Doc04. Epub 2021 May 5.

Department of Plastic, Aesthetic, Hand and Reconstructive Surgery, Hannover Medical School, Hannover, Germany.

Mucopolysaccharidosis is a rare and congenital autosomal recessive lysosomal storage disorder of glycosaminoglycans. An enzyme defect leads to cell, tissue and organ dysfunction. Carpal tunnel syndrome and trigger finger are the results of mucopolysaccharid deposition. Read More

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Protective Effects of Bone Marrow Mesenchymal Stem Cells (BMMSCS) Combined with Normothermic Machine Perfusion on Liver Grafts Donated After Circulatory Death via Reducing the Ferroptosis of Hepatocytes.

Med Sci Monit 2021 Jun 11;27:e930258. Epub 2021 Jun 11.

Department of Organ Transplantation, Tianjin First Central Hospital, Tianjin, China (mainland).

BACKGROUND To improve the quality of liver grafts from extended-criteria donors donated after circulatory death (DCD), this study explored whether bone marrow mesenchymal stem cells (BMMSCs) combined with normothermic machine perfusion (NMP) have protective effects on DCD donor livers and the effects of ferroptosis in this procedure. MATERIAL AND METHODS Twenty-four male rat DCD donor livers were randomly and averagely divided into normal, static cold storage (SCS), NMP, and NMP combined with BMMSCs groups. Liver function, bile secretion, and pathological features of DCD donor livers were detected to evaluate the protective effects of NMP and BMMSCs on DCD donor livers. Read More

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Assessment of quality characteristics and bacterial community of modified atmosphere packaged chilled pork loins using 16S rRNA amplicon sequencing analysis.

Food Res Int 2021 Jul 12;145:110412. Epub 2021 May 12.

National Center of Meat Quality and Safety Control, Synergistic Innovation Center of Food Safety and Nutrition, College of Food Science and Technology, Nanjing Agricultural University, 210095 Nanjing, Jiangsu, PR China. Electronic address:

Modified atmosphere packaging (MAP) is widely applied in packaging meat and meat products. While most studies had employed culture-dependent microbiological analyses or polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), the recent application of high-throughput sequencing (HTS) has been effective and reliable in detecting the microbial consortium associated with food spoilage. Since MAP application is limited in China, applying HTS in assessing the microbial consortium of meat and meat products in the country becomes imperative. Read More

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Metal-Organic Frameworks (MOFs) as methane adsorbents: From storage to diluted coal mining streams concentration.

Sci Total Environ 2021 Jun 4;790:148211. Epub 2021 Jun 4.

Catalysis, Reactors and Control Research Group (CRC), Department of Chemical and Environmental Engineering, University of Oviedo, Julián Clavería s/n, 33006 Oviedo, Spain. Electronic address:

Ventilation Air Methane emissions (VAM) from coal mines lead to environmental concern because their high global warming potential and the loss of methane resources. VAM upgrading requires pre-concentration processes dealing with high flow rates of very diluted streams (<1% methane). Therefore, methane separation and concentration is technically challenging and has important environmental and safety concerns. Read More

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When do apples stop growing, and why does it matter?

PLoS One 2021 10;16(6):e0252288. Epub 2021 Jun 10.

University of Reading Herbarium, School of Biological Sciences, University of Reading, Whiteknights, Reading, United Kingdom.

Apples in the commercial food chain are harvested up to two weeks before maturity. We explore apple fruit development through the growing season to establish the point at which physical features differentiating those cultivars become evident. This is relevant both for the understanding of the growing process and to ensure that any identification and classification tools can be used both on ripened-on-tree and stored fruit. Read More

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Autophagy-dependent ferroptosis as a therapeutic target in cancer.

ChemMedChem 2021 Jun 10. Epub 2021 Jun 10.

Chongqing University, School of Life Science, Shazheng Road, No. 174, Shapingba District, Chongqing, CHINA.

Ferroptosis is an iron-dependent form of cell death associated with the accumulation of labile iron and cytotoxic lipid peroxides. Increasing evidence reveals that ferroptosis isn't a self-standing phenomenon and has close connections with other cellular events. Remarkably, recent insights show that ferroptosis is dependent on autophagy, which is a lysosomal degradation pathway responsible for the recycling of damaged cellular components under survival stress. Read More

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Age of red blood cells is not associated with in-hospital mortality in massively transfused patients.

J Trauma Acute Care Surg 2021 Apr 8. Epub 2021 Apr 8.

Transfusion Research Unit, Public Health and Preventive Medicine, Monash University; Melbourne, Australia Department of Haematology, Monash Health; Melbourne, Australia Australian & New Zealand Intensive Care Research Centre, Public Health and Preventive Medicine, Monash University; Melbourne, Australia Department of Intensive Care, Western Health; Melbourne, Australia, Disclaimers: none.

Background: Studies comparing mortality following massive transfusion (MT) with fresher versus longer-stored red blood cells (RBCs) have focused on trauma patients. The Australian and New Zealand Massive Transfusion Registry (ANZMTR) collects data on all adult MT cases (≥5 RBCs within 4 hours, any bleeding context, ≥ 18 years) at participating hospitals.

Methods: 2007-2018 data from 29 hospitals were analyzed to quantify the association between mortality and RBC storage time in adult MT cases. Read More

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Quantitation of Trace Levels of DNA Released from Disrupted Adeno-Associated Virus Gene Therapy Vectors.

J Pharm Sci 2021 Jun 6. Epub 2021 Jun 6.

Formulation and Drug Product Development, REGENXBIO Inc., Rockville, MD 20850, USA.

Adeno-associated virus (AAV) vectors for gene therapy have potential to provide a durable treatment response for a number of diseases with unmet need. DNA is released from AAV capsids at high temperatures. Less is known about DNA release that may occur under conditions relevant to clinical and commercial manufacturing, storage, and distribution. Read More

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Decreased glucocerebrosidase activity and substrate accumulation of glycosphingolipids in a novel GBA1 D409V knock-in mouse model.

PLoS One 2021 9;16(6):e0252325. Epub 2021 Jun 9.

The Michael J. Fox Foundation for Parkinson's Research, Grand Central Station, New York, New York, United States of America.

Multiple mutations have been described in the human GBA1 gene, which encodes the lysosomal enzyme beta-glucocerebrosidase (GCase) that degrades glucosylceramide and is pivotal in glycosphingolipid substrate metabolism. Depletion of GCase, typically by homozygous mutations in GBA1, is linked to the lysosomal storage disorder Gaucher's disease (GD) and distinct or heterozygous mutations in GBA1 are associated with increased Parkinson's disease (PD) risk. While numerous genes have been linked to heritable PD, GBA1 mutations in aggregate are the single greatest risk factor for development of idiopathic PD. Read More

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Ocular Manifestations of Neuronal Ceroid Lipofuscinoses.

Semin Ophthalmol 2021 Jun 9:1-14. Epub 2021 Jun 9.

Department of Ophthalmology, Dayanand Medical College & Hospital, Ludhiana, India.

Neuronal ceroid lipofuscinoses (NCLs) are a group of rare neurodegenerative storage disorders associated with devastating visual prognosis, with an incidence of 1/1,000,000 in the United States and comparatively higher incidence in European countries. The pathophysiological mechanisms causing NCLs occur due to enzymatic or transmembrane defects in various sub-cellular organelles including lysosomes, endoplasmic reticulum, and cytoplasmic vesicles. NCLs are categorized into different types depending upon the underlying cause i. Read More

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Detection of Hepatitis A Virus in Strawberries Implicated in an Outbreak in the USA in 1997.

Food Environ Virol 2021 Jun 9. Epub 2021 Jun 9.

Fera Science Ltd., Sand Hutton, York, YO41 1LZ, UK.

Hepatitis A virus (HAV) was detected in frozen strawberries which had been implicated in a large outbreak of hepatitis A in 1997. The sample was analysed after over 20 years of storage, following a standard method not available at the time of the outbreak. This is the first study in which the HAV associated with the 1997 outbreak of foodborne hepatitis has finally been detected. Read More

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Impact of preoperative pelvic floor muscle training (pretraining) on urinary storage and emptying symptoms in women undergoing sling surgery.

Int Urogynecol J 2021 Jun 9. Epub 2021 Jun 9.

Department of Urology, LSU Health Shreveport, 1501 Kings Highway, Shreveport, LA, 71130, USA.

Introduction And Hypothesis: Although pelvic floor muscle training (PFMT) is an option for female mixed incontinence (MUI), the role of PFMT prior to midurethral sling (MUS) surgery is not well defined. We hypothesize that preoperative PFMT (pretraining) positively impacts urinary storage and voiding symptoms prior to retropubic MUS.

Methods: We carried out an institutional review board-approved, retrospective chart review of women with stress-predominant MUI undergoing a retropubic MUS. Read More

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Development of a method for the determination of the JAK2 gene mRNA in venous blood and assessment of its diagnostic value in oncohematology.

Klin Lab Diagn 2021 06;66(6):379-384

Krasnoyarsk branch of the «National Research Center for Hematology» Department of Health.

Overactive JAK pathway signaling is a hallmark of immune diseases and critically affects on inflammation and coagulation. A number of mutations in the JAK2 gene act as driving forces of myeloproliferative neoplasms (MPN), the pathogenesis of certain variants of acute leukemia, a number of solid malignancies and cardiovascular diseases. Assays for quantifying JAK2 mRNA in circulating blood cells can be used as a marker associated with the activity of this enzyme. Read More

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3D printed architected conducting polymer hydrogels.

J Mater Chem B 2021 Jun 8. Epub 2021 Jun 8.

Department of Materials Science and Engineering, University of California, Merced, USA. and Department of Chemistry and Chemical Biology, University of California, Merced, USA.

Conducting polymer hydrogels combine electrical conductivity and tunable water content, rendering them strong candidates for a range of applications including biosensors, cell culture platforms, and energy storage devices. However, these hydrogels are mechanically brittle and prone to damage, prohibiting their use in emerging applications involving dynamic movement and large mechanical deformation. Here, we demonstrate that applying the concept of architecture to conducting polymer hydrogels can circumvent these impediments. Read More

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The dynamics of stem water storage in the tops of Earth's largest trees-Sequoiadendron giganteum.

Tree Physiol 2021 Jun 8. Epub 2021 Jun 8.

Department of Integrative Biology, University of California, Berkeley, California 94720, USA.

Water stored in tree stems (i.e., trunks and branches) is an important contributor to transpiration that can improve photosynthetic carbon gain and reduce the probability of cavitation. Read More

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Exploring the integrity of targeted PFASs in extracted wastewater samples during transport and storage stages.

Chemosphere 2021 Jun 1;282:131065. Epub 2021 Jun 1.

Environmental Chemistry Laboratory, California Department of Toxic Substances Control (DTSC), Berkeley, CA, 94710, United States.

Little information exists on the effects of shipping and handling on per- and polyfluoroalkyl substances (PFASs) in environmental samples. Thus, we evaluated the integrity of dried wastewater extracts and the sensitivity of our high-resolution mass spectrometry (HRMS) instrument to perform such analyses by monitoring 13 representative PFASs in samples extracted, evaporated, and stored at room temperature up to one month. Relative to zero-day recoveries of six detected PFASs ranged between 94 and 124% (RSD <38%) for influents, between 88 and 126% (RSD <18%) for effluents after 28 days. Read More

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AbsoluRATE: An in-silico method to predict the aggregation kinetics of native proteins.

Biochim Biophys Acta Proteins Proteom 2021 Jun 5:140682. Epub 2021 Jun 5.

Protein Bioinformatics Lab, Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai 600036, Tamil Nadu, India; School of Computing, Institute of Innovative Research, Tokyo Institute of Technology, 4259 Nagatsutacho, Midori-ku, Yokohama, Kanagawa 226-8501, Japan. Electronic address:

Protein aggregation has two aspects, namely, mechanistic and kinetics. Understanding protein aggregation kinetics is critical for prediction of progression of diseases caused by amyloidosis, accumulation of aggregates in biotherapeutics during storage and engineering commercial nano-biomaterials. In this work, we have collected experimentally determined absolute protein aggregation rates and developed an SVM based regression model to predict absolute rates of protein and peptide aggregation near-physiological conditions. Read More

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Using the phenomenology of memory for recent events to bridge the gap between episodic and semantic memory.

Mem Cognit 2021 Jun 7. Epub 2021 Jun 7.

Department of Psychology, Colby College, Waterville, ME, 04901, USA.

Public events such as celebrity news, tragedies, and political events are widely experienced. Initially at least, memories of these events are "episodic" in nature; however, these events are also stored in associative networks similar to the semantic organization of knowledge (N. R. Read More

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Radial and axial water movement in adult trees recorded by stable isotope tracing.

Tree Physiol 2021 Jun 7. Epub 2021 Jun 7.

Hawkesbury Institute for the Environment, University of Western Sydney, Richmond, Australia.

The capacity of trees to release water from storage compartments into the transpiration stream can mitigate damage to hydraulic functioning. However, the location of these 'transient' water sources, and also the pathways of water movement other than vertical through tree stems still remains poorly understood. We conducted an experiment on two tree species in a common garden in eastern Australia that naturally grow in regions of high (Eucalyptus tereticornis, 'Red Gum') and low (Eucalyptus sideroxylon, 'Ironbark') annual precipitation rates. Read More

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International Council for Standardisation in Haematology (ICSH) recommendations for collection of blood samples for coagulation testing.

Int J Lab Hematol 2021 Jun 7. Epub 2021 Jun 7.

Christian Medical College, Vellore, India.

This guidance document has been prepared on behalf of the International Council for Standardisation in Haematology (ICSH). The aim of the document is to provide guidance and recommendations for collection of blood samples for coagulation tests in clinical laboratories throughout the world. The following processes will be covered: ordering tests, sample collection tube and anticoagulant, patient preparation, sample collection device, venous stasis before sample collection, order of draw when different sample types need to be collected, sample labelling, blood-to-anticoagulant ratio (tube filling) and influence of haematocrit. Read More

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Beneficial effects of -fermented black barley on high fat diet-induced fatty liver in rats.

Food Funct 2021 Jun 6. Epub 2021 Jun 6.

Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.

A long-term high-fat (HF) diet can cause metabolic disorders, which might induce visceral obesity and ectopic triglyceride storage (e.g., hepatic steatosis), and increase hepatic oxidative stress. Read More

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Delving into possible missing links for attainment of food security in Central Malawi: farmers' perceptions and long term dynamics in maize ( L.) production.

Heliyon 2021 May 24;7(5):e07130. Epub 2021 May 24.

Ministry of Agriculture, Salima Agricultural Development Division, Private Bag 1, Salima, Malawi.

Globally, maize is one of the major cereal crops contributing 30% of food calories to about 4.6 billion people. The crop is considered staple in 125 developing countries where majority of producers are smallholder farmers. Read More

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Carp Edema Virus Infection Is Associated With Severe Metabolic Disturbance in Fish.

Front Vet Sci 2021 19;8:679970. Epub 2021 May 19.

Department of Ecology and Diseases of Zoo Animals, Game, Fish and Bees, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czechia.

Significant mortalities associated with emerging viral diseases are challenging the economy of common carp aquaculture. As such, there is an increased need to disentangle how infected fish cope with progressive disease pathology and lose the ability for homeostatic maintenance of key physiological parameters. A natural carp edema virus (CEV) infection outbreak at a carp fish farm provided an opportunity to examine diseased and healthy carp in the same storage pond, thereby contributing to our better understanding of CEV disease pathophysiology. Read More

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