610 results match your criteria stoichiometry microbial


Plant mixture balances terrestrial ecosystem C:N:P stoichiometry.

Nat Commun 2021 07 27;12(1):4562. Epub 2021 Jul 27.

Faculty of Natural Resources Management, Lakehead University, Thunder Bay, ON, Canada.

Plant and soil C:N:P ratios are of critical importance to productivity, food-web dynamics, and nutrient cycling in terrestrial ecosystems worldwide. Plant diversity continues to decline globally; however, its influence on terrestrial C:N:P ratios remains uncertain. By conducting a global meta-analysis of 2049 paired observations in plant species mixtures and monocultures from 169 sites, we show that, on average across all observations, the C:N:P ratios of plants, soils, soil microbial biomass and enzymes did not respond to species mixture nor to the species richness in mixtures. Read More

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Short-term effects of grazing intensity on soil stoichiometric characteristics of typical grassland in the agro-pastoral ecotone of northern China.

Ying Yong Sheng Tai Xue Bao 2021 Jul;32(7):2433-2439

College of Grassland Science, Shanxi Agricultural University, Jinzhong 030801, Shanxi, China.

Grazing is the dominant land use way for natural grasslands. Different grazing intensities could affect soil stoichiometry in grasslands by influencing the selective feeding by livestock, litter input, and microbial community structure. In this study, a grazing experiment was carried out in a grassland of agro-pastoral ecotone in Northern China for three years (2017-2019). Read More

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C:N:P stoichiometry responses to 10 years of nitrogen addition differ across soil components and plant organs in a subtropical Pleioblastus amarus forest.

Sci Total Environ 2021 Jul 8;796:148925. Epub 2021 Jul 8.

Key Laboratory of National Forestry, Grassland Administration on Forest Resources Conservation and Ecological Safety in the Upper Reaches of the Yangtze River, College of Forestry, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Electronic address:

How stoichiometry in different ecosystem components responds to long-term nitrogen (N) addition is crucial for understanding within-ecosystem biogeochemistry cycling processes in the context of global change. To explore the effects of long-term N addition on nutrient stoichiometry in soil and plant components in forest ecosystem, a 10-year N addition experiment using ammonium nitrate (NHNO) was conducted in a bamboo forest in the Rainy Zone of West China, where the background N deposition is the highest in the world. Four N treatment levels (+0, +50, +150, +300 kg N ha yr) (CK, LN, MN, HN) were applied monthly since November 2007, and then, the C:N:P stoichiometry of soil, microbial biomass, and enzymes in rhizosphere soil and bulk soil, and plant organs were measured. Read More

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Product Inhibition and pH Affect Stoichiometry and Kinetics of Chain Elongating Microbial Communities in Sequencing Batch Bioreactors.

Front Bioeng Biotechnol 2021 21;9:693030. Epub 2021 Jun 21.

Department of Biotechnology, Delft University of Technology, Delft, Netherlands.

Anaerobic microbial communities can produce carboxylic acids of medium chain length (e.g., caproate, caprylate) by elongating short chain fatty acids through reversed β-oxidation. Read More

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Resource stoichiometry, vegetation type and enzymatic activity control wetlands soil organic carbon in the Herbert River catchment, North-east Queensland.

J Environ Manage 2021 Jul 3;296:113183. Epub 2021 Jul 3.

Australian Rivers Institute and School of Environment and Science, Griffith University, Nathan, QLD, 4111, Australia. Electronic address:

Wetlands are highly productive ecosystem with great potential to store carbon (C) and retain nitrogen (N) and phosphorus (P) in their soil. Changes in vegetation type and land use can affect organic matter inputs and soil properties. This work aimed to examine how these changes affected elemental stoichiometry and C-, N-, and P- associated enzyme activities and wetland soil organic C stock. Read More

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Respirometry tests in wastewater treatment: Why and how? A critical review.

Sci Total Environ 2021 Jun 24;793:148607. Epub 2021 Jun 24.

Department Polytechnic of Engineering and Architecture, University of Udine, Via del Cotonificio 108, 33100 Udine, Italy.

Respirometry tests are a widely employed method in wastewater treatment field to characterize wastewater streams, assess toxic/inhibitory effects to the biomass, calibrate mathematical models. Respirometry can allow to fractionize the chemical oxygen demand (COD) in biodegradable and inert fractions, but also provide information related to biomass kinetics and stoichiometry through standardized laboratory techniques. Considering the increasing number of emerging contaminants detected in wastewater effluents, such as pharmaceuticals, personal care products and pesticides, respirometry can be a useful tool to promptly assess any toxic or inhibitory effect in wastewater treatment plants (WWTPs) operations. Read More

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Long-term geothermal warming reduced stocks of carbon but not nitrogen in a subarctic forest soil.

Glob Chang Biol 2021 Jun 22. Epub 2021 Jun 22.

Thünen Institute of Climate-Smart Agriculture, Braunschweig, Germany.

Global warming is accelerating the decomposition of soil organic matter (SOM). When predicting the net SOM dynamics in response to warming, there are considerable uncertainties owing to experimental limitations. Long-term in situ whole-profile soil warming studies are particularly rare. Read More

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Conditions stimulating neutral detergent fiber degradation by dosing branched-chain volatile fatty acids. II: Relation with solid passage rate and pH on neutral detergent fiber degradation and microbial function in continuous culture.

J Dairy Sci 2021 Jun 16. Epub 2021 Jun 16.

Department of Animal Sciences, The Ohio State University, Columbus 43210. Electronic address:

To support improving genetic potential for increased milk production, intake of digestible carbohydrate must also increase to provide digestible energy and microbial protein synthesis. We hypothesized that the provision of exogenous branched-chain volatile fatty acids (BCVFA) would improve both neutral detergent fiber (NDF) degradability and efficiency of microbial protein synthesis. However, BCVFA should be more beneficial with increasing efficiency of bacterial protein synthesis associated with increasing passage rate (k). Read More

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Increasing electron donor concentration does not accelerate complete microbial reductive dechlorination in contaminated sediment with native organic carbon.

Biodegradation 2021 Jun 3. Epub 2021 Jun 3.

Environmental Engineering and Earth Sciences, Clemson University, 312 Biosystems Research Complex, 105 Collings Street, Clemson, SC, 29634, USA.

Experiments with Fe(III)-rich, chloroethene-contaminated sediment demonstrated that trichloroethylene (TCE) and vinyl chloride (VC) were completely reduced to ethene regardless of whether electron donor(s) were added at 1 × stoichiometry or 10 × stoichiometry relative to all-electron acceptors. Unamended controls uniformly reduced TCE to ethene with a mean time to complete dechlorination (operationally defined as the presence of stoichiometric ethene production) of 79 days. Adding 1 × and 10 × acetate hindered the rate and extent of TCE and VC reduction relative to unamended controls, with several only partially reduced when the experiments were terminated. Read More

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Biochar application under low phosphorus input promotes soil organic phosphorus mineralization by shifting bacterial phoD gene community composition.

Sci Total Environ 2021 Jul 18;779:146556. Epub 2021 Mar 18.

College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Key Laboratory of Eco-Circular Agriculture, South China Agricultural University, Guangzhou 510642, China. Electronic address:

Biochar has the potential to enhance microbial-mediated phosphorus (P) cycling in soils, but the underlying mechanisms remain largely unknown. We hypothesized that biochar amendment could enhance the production of acid and alkaline phosphomonoesterase, phosphodiesterase and P mineralization, which may vary depending on the P input. To test this hypothesis, we assessed the impacts of rice straw biochar application (0 and 4%) under different P-input rates (0, 30 and 90 kg P ha) on the relationships among P fractions, phosphatase activities and alkaline phosphomonoesterase-encoding bacterial (phoD gene) communities in an acidic soil. Read More

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Substrate control of sulphur utilisation and microbial stoichiometry in soil: Results of C, N, C, and S quad labelling.

ISME J 2021 May 11. Epub 2021 May 11.

School of Natural Sciences, Bangor University, Gwynedd, UK.

Global plant sulphur (S) deficiency is increasing because of a reduction in sulphate-based fertiliser application combined with continuous S withdrawal during harvest. Here, we applied C, N, C, and S quad labelling of the S-containing amino acids cysteine (Cys) and methionine (Met) to understand S cycling and microbial S transformations in the soil. The soil microorganisms absorbed the applied Cys and Met within minutes and released SO within hours. Read More

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Long-term continuous cropping affects ecoenzymatic stoichiometry of microbial nutrient acquisition: a case study from a Chinese Mollisol.

J Sci Food Agric 2021 May 10. Epub 2021 May 10.

Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, China.

Background: Soil- and plant-produced extracellular enzymes drive nutrient cycling in soils and are assumed to regulate supply and demand for carbon (C) and nutrients within the soil. Thus, agriculture management decisions that alter the balance of plant and supplemental nutrients should directly alter extracellular enzyme activities (EEAs), and EEA stoichiometry in predictable ways. We used a 12-year experiment that varyied three major continuous grain crops (wheat, soybean, and maize), each crossed with mineral fertilizer (WCF, SCF and MCF, respectively) or not fertilized (WC, SC and MC, respectively, as controls). Read More

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Shrubs magnify soil phosphorus depletion in Tibetan meadows: Conclusions from C:N:P stoichiometry and deep soil profiles.

Sci Total Environ 2021 Sep 24;785:147320. Epub 2021 Apr 24.

Department of Soil Science of Temperate Ecosystems, Department of Agricultural Soil Science, University of Göttingen, Göttingen, Germany.

Globally, the proliferation of shrubs within grasslands stimulates soil phosphorus (P) cycling and increases topsoil P storage beneath their canopies. However, little is known regarding the impact of shrub encroachment on subsoil P storage, and whether shrubs mediate changes in soil stoichiometry, like increasing P cycling. In grazed meadows on the Tibetan Plateau, soil and roots were sampled to 1 m depth in shrubby Hippophae rhamnoides ssp. Read More

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September 2021

Brown meets green: light and nutrients alter detritivore assimilation of microbial nutrients from leaf litter.

Ecology 2021 06 24;102(6):e03358. Epub 2021 May 24.

School of Biological, Environmental, and Earth Sciences, University of Southern Mississippi, Hattiesburg, Mississippi, 39406, USA.

In aquatic detrital-based food webs, research suggests that autotroph-heterotroph microbial interactions exert bottom-up controls on energy and nutrient transfer. To address this emerging topic, we investigated microbial responses to nutrient and light treatments during Liriodendron tulipifera litter decomposition and fed litter to the caddisfly larvae Pycnopsyche sp. We measured litter-associated algal, fungal, and bacterial biomass and production. Read More

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Organic substitutions aggravated microbial nitrogen limitation and decreased nitrogen-cycling gene abundances in a three-year greenhouse vegetable field.

J Environ Manage 2021 Jun 23;288:112379. Epub 2021 Mar 23.

Jiangsu Key Laboratory of Low Carbon Agriculture and GHGs Mitigation, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address:

Partially substituting chemical fertilizer with organic fertilizer has substantially changed the stoichiometric imbalances of carbon (C), nitrogen (N) and phosphorus (P) between microbial communities and their available resources in agroecosystems. However, how organic substitution alters microbial nutrient limitation and then affects soil N cycle in intensive greenhouse vegetable ecosystem, remain unknown. Thus, we performed a three-year greenhouse vegetable field experiment in China with different fertilization strategies: no N fertilization, chemical N fertilization, and substituting 20% (1M4N) or 50% (1M1N) of chemical N with organic fertilizer (organic substitutions). Read More

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Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers.

J Vis Exp 2021 03 4(169). Epub 2021 Mar 4.

Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory;

Histones belong to a family of highly conserved proteins in eukaryotes. They pack DNA into nucleosomes as functional units of chromatin. Post-translational modifications (PTMs) of histones, which are highly dynamic and can be added or removed by enzymes, play critical roles in regulating gene expression. Read More

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Enzyme stoichiometry evidence revealed that five years nitrogen addition exacerbated the carbon and phosphorus limitation of soil microorganisms in a forest.

Ying Yong Sheng Tai Xue Bao 2021 Feb;32(2):521-528

School of Geographical Science, Fujian Normal University, Fuzhou 350007, China.

The activity and stoichiometry of soil extracellular enzyme can provide a good indication for changes in soil nutrient availability and microbial demands for nutrients. However, it remains unclear how would nitrogen (N) deposition affect nutrient limitation of microbes in subtropical forest soils. We conducted a 5 years N addition experiment in a subtropical forest. Read More

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February 2021

Soil N availability drives the shifts of enzyme activity and microbial phosphorus limitation in the artificial soil on cut slope in southwestern China.

Environ Sci Pollut Res Int 2021 Feb 27. Epub 2021 Feb 27.

Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, Sichuan, 610065, People's Republic of China.

The construction of highways in the subalpine mountains generates many cut slopes. Currently, the restoration of cut slope mainly focuses on the aboveground landscapes and slope stability. Yet, it remains elusive about the belowground ecosystem functions at the early stage of restoration. Read More

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February 2021

Reductive Transformation of Fe(III) (oxyhydr)Oxides by Mesophilic Homoacetogens in the Genus .

Front Microbiol 2021 1;12:600808. Epub 2021 Feb 1.

Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, Japan.

Microbial reduction of iron contributes to the dissolution and transformation of iron-containing minerals in nature. Diverse groups of homoacetogenic bacteria (homoacetogens) have been reported to reduce insoluble Fe(III) oxides, such as hydrous ferric oxide (HFO), an Fe(III) mineral commonly found in soils and sediments. Several members of genus reportedly oxidize Fe(0), indicating the presence of an extracellular electron-uptake mechanism. Read More

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February 2021

Structural characterization of Myxococcus xanthus MglC, a component of the polarity control system, and its interactions with its paralog MglB.

J Biol Chem 2021 Jan-Jun;296:100308. Epub 2021 Jan 22.

Structural Biology Laboratory, Council of Scientific and Industrial Research-Institute of Microbial Technology, G. N. Ramachandran Protein Centre, Chandigarh, India. Electronic address:

The δ-proteobacteria Myxococcus xanthus displays social (S) and adventurous (A) motilities, which require pole-to-pole reversal of the motility regulator proteins. Mutual gliding motility protein C (MglC), a paralog of GTPase-activating protein Mutual gliding motility protein B (MglB), is a member of the polarity module involved in regulating motility. However, little is known about the structure and function of MglC. Read More

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January 2021

Impact of on Phthalides Accumulation in (Oliv.) by Stoichiometry and Microbial Diversity Analysis.

Front Microbiol 2020 8;11:611143. Epub 2021 Jan 8.

Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing, China.

Plant-microorganism interaction in the rhizosphere is thought to play an important role in the formation of soil fertility, transformation and absorption of nutrients, growth and development of medicinal plants, and accumulation of medicinal ingredients. Yet, the role that they play in the phthalides accumulation of (Oliv.) Diels remains unclear. Read More

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January 2021

Nutrient-imbalanced conditions shift the interplay between zooplankton and gut microbiota.

BMC Genomics 2021 Jan 7;22(1):37. Epub 2021 Jan 7.

Department of Ocean Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, SAR, China.

Background: Nutrient stoichiometry of phytoplankton frequently changes with aquatic ambient nutrient concentrations, which is mainly influenced by anthropogenic water treatment and the ecosystem dynamics. Consequently, the stoichiometry of phytoplankton can markedly alter the metabolism and growth of zooplankton. However, the effects of nutrient-imbalanced prey on the interplay between zooplankton and their gut microbiota remain unknown. Read More

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January 2021

Tuning the Surface Interactions between Single Cells and an OSTE+ Microwell Array for Enhanced Single Cell Manipulation.

ACS Appl Mater Interfaces 2021 Jan 7;13(2):2316-2326. Epub 2021 Jan 7.

Department of Biosystems, Biosensors Group, KU Leuven, Willem De Croylaan 42, Leuven 3001, Belgium.

Retrieving single cells of interest from an array of microwells for further off-chip analysis is crucial in numerous biological applications. To this end, several single cell manipulation strategies have been developed, including optical tweezers (OT). OT represent a unique approach for contactless cell retrieval, but their performance is often suboptimal due to nonspecific cell adhesion to the microwell surface. Read More

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January 2021

Filter-feeders have differential bottom-up impacts on green and brown food webs.

Oecologia 2021 Jan 2;195(1):187-198. Epub 2021 Jan 2.

Department of Crop, Soil and Environmental Sciences, Auburn University, Auburn, AL, 36849, USA.

Nutrient recycling by consumers can strongly impact nutrient availability for autotrophic and heterotrophic microbes, thus impacting functions such as primary production and decomposition. Filter-feeding freshwater mussels form dense, multispecies assemblages in aquatic ecosystems and have been shown to play a critical role in nutrient cycling. Mussel excretion can enhance benthic primary production and influence algal species composition. Read More

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January 2021

[Effects of Farmland Abandonment on Soil Enzymatic Activity and Enzymatic Stoichiometry in the Loess Hilly Region, China].

Huan Jing Ke Xue 2021 Jan;42(1):411-421

College of Agronomy, Northwest A&F University, Yangling 712100, China.

Clarifying the characteristic of soil enzymatic activity and stoichiometry variations as well as their influencing factors following farmland abandonment have important implications for understanding soil nutrient availability after revegetation and for illuminating the underlying mechanisms of soil nutrient cycling in ecosystems. To determine microbial nutrient limitations after farmland abandonment and to explore the driving factors of the variations in soil enzymatic activity and stoichiometry along a chronosequence of abandoned farmlands (0-, 10-, 20-, and 30-year-old) in the Loess Hilly Region, China, the potential activities of carbon (C)-, nitrogen (N)-, and phosphorus (P)-acquiring enzymes, soil physicochemical properties, and plant diversity and family composition were measured. The results showed that the activities of -1,4--acetylglucosaminidase (NAG), leucine aminopeptidase (LAP), and alkaline phosphatase (ALP) increased significantly with the increasing years of land abandonment, whereas the activity of -1,4-glucosidase (BG) showed the opposite change trend. Read More

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January 2021

Patterns and Drivers of Extracellular Enzyme Activity in New Zealand Glacier-Fed Streams.

Front Microbiol 2020 19;11:591465. Epub 2020 Nov 19.

Stream Biofilm and Ecosystem Research Laboratory, School of Architecture, Civil and Environmental Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

Glacier-fed streams (GFSs) exhibit near-freezing temperatures, variable flows, and often high turbidities. Currently, the rapid shrinkage of mountain glaciers is altering the delivery of meltwater, solutes, and particulate matter to GFSs, with unknown consequences for their ecology. Benthic biofilms dominate microbial life in GFSs, and play a major role in their biogeochemical cycling. Read More

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November 2020

[Effects of broadleaved tree species on soil microbial stoichiometry in clear-cut patches of plantation].

Ying Yong Sheng Tai Xue Bao 2020 Nov;31(11):3851-3858

School of Geographical Science, Fujian Normal University, Fuzhou 350007, China.

Investigating the response of soil microbial biomass and ecological stoichiometry to tree species transition is of great significance for understanding soil nutrient cycling and availability in forest ecosystems. We measured soil microbial biomass carbon (MBC), nitrogen (MBN), phosphorus (MBP) and their stoichiometry across 0-40 cm soil depth between and plantations by the chloroform fumigation extraction method, which were replanted after the harvest of plantation. The results showed that soil MBC in the 0-10 cm layer and soil MBN and MBP in the 0-20 cm layer under the were significantly higher than those under the . Read More

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November 2020

Effects of tree species and topography on soil and microbial biomass stoichiometry in Funiu Mountain, China.

BMC Ecol 2020 12 9;20(1):67. Epub 2020 Dec 9.

Forestry College, Henan University of Science & Technology, No. 263 Kaiyuan Avenue, Luoyang, 471000, China.

Background: Soil and microbial biomass stoichiometry plays an important role in understanding nutrient cycling in terrestrial ecosystems. However, studies on soil and microbial biomass stoichiometry in forests are rare. This study investigated the effect of tree species and topographic factors on the ecological stoichiometry of soil and soil microbial biomass. Read More

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December 2020

Enhanced biological nitrogen removal under low dissolved oxygen in an anaerobic-anoxic-oxic system: Kinetics, stoichiometry and microbial community.

Chemosphere 2021 Jan 29;263:128184. Epub 2020 Aug 29.

Department of Civil, Architectural and Environmental Engineering, Missouri University of Science and Technology, Rolla, MO, 65409, USA.

A lab-scale anaerobic-anoxic-oxic system was used to investigate the nitrogen removal mechanism under low dissolved oxygen (DO) conditions. When DO was decreased from 2 to 0.5 mg L, chemical oxygen demand (COD) and NH removals were not influenced, while total nitrogen removal increased from 69% to 79%. Read More

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January 2021

Effects of terrigenous organic substrates and additional phosphorus on bacterioplankton metabolism and exoenzyme stoichiometry.

Freshw Biol 2020 Nov 13;65(11):1973-1988. Epub 2020 Jul 13.

Institute of Hydrobiology and Aquatic Ecosystem Management University of Natural Resources and Life Sciences (BOKU) Vienna Austria.

Bamboo, as a pioneer vegetation, often forms forests on bare lands after catastrophic landslides. Compared to evergreen forest soil, bamboo forest soil is much more labile, with a higher percentage of microbially derived organic carbon (OC), lower molecular weight, and lower humic acid content. We hypothesised that different terrigenous organic matter (tOM) sources with varying lability and phosphorus (P) availability select for bacterioplankton with distinct metabolic pathways. Read More

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November 2020