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Target molecules for future hidradenitis suppurativa treatment.

Exp Dermatol 2021 Jun;30 Suppl 1:8-17

European Hidradenitis Suppurativa Foundation e.V., Dessau, Germany.

The registration of the tumour necrosis factor-α inhibitor adalimumab in 2015 was a major step forward in the treatment of hidradenitis suppurativa/acne inversa (HS). However, it soon became evident that the effectiveness of adalimumab in daily practice was highly variable. A significant unmet medical need of HS patients remained, and the search for novel therapeutic targets was intensified. Read More

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Workload Accomplished in Phase III Cardiac Rehabilitation.

J Funct Morphol Kinesiol 2021 May 28;6(2). Epub 2021 May 28.

Department of Exercise and Sport Science, University of Wisconsin-La Crosse, La Crosse, WI 54601, USA.

Exercise training is an important component of clinical exercise programs. Although there are recognized guidelines for the amount of exercise to be accomplished (≥70,000 steps per week or ≥150 min per week at moderate intensity), there is virtually no documentation of how much exercise is actually accomplished in contemporary exercise programs. Having guidelines without evidence of whether they are being met is of limited value. Read More

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Membrane Interference Against HIV-1 by Intrinsic Antiviral Factors: The Case of IFITMs.

Cells 2021 May 11;10(5). Epub 2021 May 11.

Centre International de Recherche en Infectiologie (CIRI), Université de Lyon, Inserm U1111, CNRS, UMR5308, ENS de Lyon, Université Claude Bernard Lyon 1, 46 Allée d'Italie, 69007 Lyon, France.

HIV-1 is a complex retrovirus that is adapted to replicate in cells of the immune system. To do so, HIV-1, like other viruses, developed strategies to use several cellular processes to its advantage, but had also to come to terms with an arsenal of cellular innate defense proteins, or antiviral factors, that target more or less efficiently, virtually every step of the virus replicative cycle. Among antiviral restriction factors, the family of interferon-induced transmembrane proteins (IFITMs) has emerged as a crucial component of cellular innate defenses for their ability to interfere with both early and late phases of viral replication by inhibiting cellular and viral membranes fusion. Read More

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STEP® vectors for rapid generation of stable transfected CHO cell pools and clones with high expression levels and product quality homogeneity of difficult-to-express proteins.

Protein Expr Purif 2021 May 24:105920. Epub 2021 May 24.

Batavia Biosciences Inc., 300 Trade Center Suite 6650, Woburn, MA, USA. Electronic address:

Many proteins produced in CHO cells need evaluation for their clinical and commercial potential. Traditional methods based on stable clone generation are slow and unsuitable for screening larger numbers of proteins, while transient expression technologies are fast but unpredictable regarding product quality and lacking an optional path to subcloning. The STEP® vector technology introduced here combines the best properties of both methods. Read More

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Virtual Multiple Mini-Interviews for Veterinary Admissions.

J Vet Med Educ 2021 May 25:e20200107. Epub 2021 May 25.

Admissions teams are challenged to select the best applicants for their college. There is a growing emphasis on selecting applicants with personal attributes important for success in a variety of veterinary careers, but there is no clear consensus on how to best identify these individuals. A number of veterinary colleges are utilizing multiple mini-interviews (MMIs), a highly structured type of interview in this selection process. Read More

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Air-Processed MAPbBr Perovskite Thin Film with Ultrastability and Enhanced Amplified Spontaneous Emission.

Small 2021 May 21:e2101107. Epub 2021 May 21.

School of Physics, Chongqing University, Chongqing, 401331, China.

The poor stability, in particular with respect to temperature, moisture, and light exposure, remains a ubiquitous impediment virtually for metal halide perovskite materials and devices in their future practical application. Herein, from the perspective of precursor solution chemistry, ionic liquid solvent methylammonium acetate (MAAc) is introduced to prepare high-quality MAPbBr perovskite thin films in a one-step air-processing process without anti-solvent treatment. Due to formation of pinhole-free, uniform, and compact MAPbBr perovskite film, excellent amplified spontaneous emission (ASE) with high emission efficiency and low threshold is obtained under nanosecond laser. Read More

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Multi-step vs. single-step resistance evolution under different drugs, pharmacokinetics, and treatment regimens.

Elife 2021 May 18;10. Epub 2021 May 18.

Institute of Integrative Biology, ETH Zurich, Zurich, Switzerland.

The success of antimicrobial treatment is threatened by the evolution of drug resistance. Population genetic models are an important tool in mitigating that threat. However, most such models consider resistance emergence via a single mutational step. Read More

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Actions and interactions of IGF-I and MMPs during muscle regeneration.

Semin Cell Dev Biol 2021 May 4. Epub 2021 May 4.

Applied Physiology & Kinesiology, College of Health and Human Performance, University of Florida, 1864 Stadium Road, Gainesville, FL 32611, USA. Electronic address:

Muscle regeneration requires the coordination of several factors to mobilize satellite cells and macrophages, remodel the extracellular matrix surrounding muscle fibers, and repair existing and/or form new muscle fibers. In this review, we focus on insulin-like growth factor I and the matrix metalloproteinases, which are secreted proteins that act on cells and the matrix to resolve damage. While their actions appear independent, their interactions occur at the transcriptional and post-translational levels to promote feed-forward activation of each other. Read More

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Digital synthesis of histological stains using micro-structured and multiplexed virtual staining of label-free tissue.

Light Sci Appl 2020 May 6;9(1):78. Epub 2020 May 6.

Electrical and Computer Engineering Department, University of California, Los Angeles, CA, 90095, USA.

Histological staining is a vital step in diagnosing various diseases and has been used for more than a century to provide contrast in tissue sections, rendering the tissue constituents visible for microscopic analysis by medical experts. However, this process is time consuming, labour intensive, expensive and destructive to the specimen. Recently, the ability to virtually stain unlabelled tissue sections, entirely avoiding the histochemical staining step, has been demonstrated using tissue-stain-specific deep neural networks. Read More

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Identification of chemicals of emerging concern in urine of Flemish adolescents using a new suspect screening workflow for LC-QTOF-MS.

Chemosphere 2021 Apr 27;280:130683. Epub 2021 Apr 27.

Toxicological Center, University of Antwerp, Universiteitsplein 1, 2610, Wilrijk, Belgium. Electronic address:

An essential step in human biomonitoring or molecular epidemiology programs is to estimate human exposure to environmental chemicals. Despite significant progress in the capabilities of analytical methods, the number of pollutants and their metabolites keeps increasing continuously. Some of these relatively unknown chemicals of emerging concern (CECs) may pose significant health risks to humans and biota, but remain virtually undetected in traditional HBM-studies. Read More

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Interpersonal Perception of Time-Use Patterns in Romantic Relationships: Protocol for the IP-COUPLES Study.

JMIR Res Protoc 2021 May 4;10(5):e21306. Epub 2021 May 4.

AGEIS, University Grenoble Alpes, Grenoble, France.

Background: Perceptual congruence has been defined as the level of agreement between partners on various aspects of their shared lives, including perceived engagement in individual and jointly performed activities. While the level of adjustment made by partners to such activities is thought to contribute to a couple's sense of mutuality, perceptions of time use concerning activity engagement has yet to be considered. As such, this study will determine the level of perceptual congruence between partners with respect to perceived time use in their respective and shared activities. Read More

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Elastic Image Fusion Software to Coregister Preoperatively Planned Pedicle Screws With Intraoperative Computed Tomography Data for Image-Guided Spinal Surgery.

Int J Spine Surg 2021 Apr 23;15(2):295-301. Epub 2021 Mar 23.

Weill Cornell Brain and Spine Center, Department of Neurological Surgery, Weill Cornell Medical College, New York Presbyterian Hospital, New York, New York.

Background: For complex spinal cases, especially when robotic guidance is used, preoperative planning of pedicle screws can be helpful. Transfer of these preoperatively planned pedicle screws to intraoperative 3-dimensional imaging is challenging because of changes in anatomic alignment between preoperative supine and intraoperative prone imaging, especially when multiple levels are involved. In the spine, where each individual vertebra is subject to independent movement from adjacent level, rigid image fusion is confined to a single vertebra and can display fusion inaccuracies on adjacent levels. Read More

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Interdisciplinary guided dentistry, digital quality control, and the "copy-paste" concepts.

J Esthet Restor Dent 2021 Apr 26. Epub 2021 Apr 26.

Private Practice, São Paulo, Brazil.

Objective: The aim of this report is to present an interdisciplinary approach with novel concepts to virtually plan and achieve esthetics and function.

Clinical Considerations: Despite the advancements in the digital workflow applied to restorative dentistry, the final outcomes are commonly not similar to initial planning. To overcome this major limitation, three concepts are proposed: guided dentistry, digital quality control and "copy-paste" dentistry. Read More

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Web-based Medical Education During COVID-19 Lockdown: A Step Back or a Leap to the Future?

Int J Low Extrem Wounds 2021 Apr 23:15347346211011848. Epub 2021 Apr 23.

37791Department of Vascular Surgery, Democritus University of Thrace, Alexandroupolis, Greece.

The impact of coronavirus infectious disease (COVID-19) on medical education has been substantial. Medical students require considerable clinical exposure. However, due to the risk of COVID-19, the majority of medical schools globally have discontinued their normal activities. Read More

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MitopatHs: a new logically-framed tool for visualizing multiple mitochondrial pathways.

iScience 2021 Apr 19;24(4):102324. Epub 2021 Mar 19.

Department of Neuroscience and Rehabilitation, University of Ferrara, Ferrara, Italy.

Mitochondria are key organelles inside the cell that house a wide range of molecular pathways involved in energy metabolism, ions homeostasis, and cell death. Several databases characterize the different mitochondrial aspects and thus support basic and clinical research. Here we present MitopatHs, a web-based data set that allows navigating among the biochemical signaling pathways (PatHs) of human (H) mitochondria (Mito). Read More

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Effects of nasopharyngeal microbiota in respiratory infections and allergies.

Clin Exp Pediatr 2021 Apr 15. Epub 2021 Apr 15.

Division of Pediatric Infectious Diseases, Departments of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea.

The human microbiome, which consists of a collective cluster of commensal, symbiotic, and pathogenic microorganisms living in the human body, plays a key role in host health and immunity. The human nasal cavity harbors commensal bacteria that suppress the colonization of opportunistic pathogens. However, dysbiosis of the nasal microbial community is associated with many diseases, such as acute respiratory infections including otitis media, sinusitis and bronchitis and allergic respiratory diseases including asthma. Read More

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Overlapping Allografts Provide Superior and More Reliable Surface Topography Matching Than Oblong Allografts: A Computer-Simulated Model Study.

Am J Sports Med 2021 05 8;49(6):1505-1511. Epub 2021 Apr 8.

Rush University Medical Center, Chicago, Illinois, USA.

Background: Osteochondral allograft transplantation is 1 treatment option for focal articular cartilage defects of the knee. Large irregular defects, which can be treated using an oblong allograft or multiple overlapping allografts, increase the procedure's technical complexity and may provide suboptimal cartilage and subchondral surface matching between donor grafts and recipient sites.

Purpose: To quantify and compare cartilage and subchondral surface topography mismatch and cartilage step-off for oblong and overlapping allografts using a 3-dimensional simulation model. Read More

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Photoredox Catalytic Pentafluorosulfanylative Domino Cyclization of α-Substituted Alkenes to Oxaheterocycles by Using SF.

Chemistry 2021 Jun 11;27(31):8088-8093. Epub 2021 May 11.

Institute of Organic Chemistry, Karlsruhe Institute of Technology (KIT), Fritz-Haber-Weg 6, 76131, Karlsruhe, Germany.

Virtually inert sulfur hexafluoride becomes a precious pentafluorosulfanylation agent, if properly activated by photoredox catalysis, to access α-fluoro and α-alkoxy SF -compounds. This advanced protocol converts SF in the presence of alkynols as bifunctional C-C- and C-O-bond forming reagents directly into pentafluorosulfanylated oxygen-containing heterocycles in a single step from α-substituted alkenes. The proposed mechanism is supported by theoretical calculations and gives insights not only in the pentafluorosulfanylation step but also into formation of the carbon-carbon bond and is in full agreement with Baldwin's cyclization rules. Read More

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Application field of VOXEL-MAN Tempo 3D virtual reality simulator in surgery of pars petrosa of temporal bone

Orv Hetil 2021 04 7;162(16):623-628. Epub 2021 Apr 7.

1 Szegedi Tudományegyetem, Általános Orvostudományi Kar, Fül-Orr-Gégészeti és Fej-Nyaksebészeti Klinika, Szeged, Tisza Lajos krt. 111., 6725.

Összefoglaló. Bevezetés: Az emberi sziklacsont a halántékcsont része, egy bonyolult és változatos anatómiai felépítésű struktúra. A sziklacsonton végzett beavatkozások előtt, a műtéti szövődmények megelőzése érdekében, nélkülözhetetlen a biztos anatómiai tudás és kézügyesség megszerzése, valamint az egyes műtéti lépések és mozdulatok begyakorlása. Read More

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β-Amyrin Synthase1 Controls the Accumulation of the Major Saponins Present in Pea (Pisum sativum).

Plant Cell Physiol 2021 Apr 7. Epub 2021 Apr 7.

Agroécologie, AgroSup Dijon, INRAE, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France.

The use of pulses as ingredients for the production of food products rich in plant proteins is increasing. However, protein fractions prepared from pea or other pulses contain significant amounts of saponins, glycosylated triterpenes which can impart an undesirable bitter taste when used as an ingredient in foodstuffs. In this paper, we describe the identification and characterization of a gene involved in saponin biosynthesis during pea seed development, by screening mutants obtained from two Pisum sativum TILLING (Targeting Induced Local Lesions in Genomes) populations in two different genetic backgrounds. Read More

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High social status males experience accelerated epigenetic aging in wild baboons.

Elife 2021 Apr 6;10. Epub 2021 Apr 6.

Department of Evolutionary Anthropology, Duke University, Durham, United States.

Aging, for virtually all life, is inescapable. However, within populations, biological aging rates vary. Understanding sources of variation in this process is central to understanding the biodemography of natural populations. Read More

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The Natural Historian's Guide to the CT Galaxy: Step-by-Step Instructions for Preparing and Analyzing Computed Tomographic (CT) Data Using Cross-Platform, Open Access Software.

Integr Org Biol 2020 10;2(1):obaa009. Epub 2020 Apr 10.

Department of Biology and SAFS, University of Washington, Friday Harbor Laboratories, Friday Harbor, Washington, DC, USA.

The decreasing cost of acquiring computed tomographic (CT) data has fueled a global effort to digitize the anatomy of museum specimens. This effort has produced a wealth of open access digital three-dimensional (3D) models of anatomy available to anyone with access to the Internet. The potential applications of these data are broad, ranging from 3D printing for purely educational purposes to the development of highly advanced biomechanical models of anatomical structures. Read More

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Identification of immucillin analogue natural compounds to inhibit MTAN through high throughput virtual screening and molecular dynamics simulation.

In Silico Pharmacol 2021 11;9(1):22. Epub 2021 Mar 11.

Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu 627 012 India.

Abstract: One in every two humans is having () in stomach causing gastric ulcer. Emergence of several drugs in eliminating has paved way for emergence of multidrug resistance in them. This resistance is thriving and thereby necessitating the need of a potent drug. Read More

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What defines a synthetic riboswitch? - Conformational dynamics of ciprofloxacin aptamers with similar binding affinities but varying regulatory potentials.

Nucleic Acids Res 2021 04;49(7):3661-3671

Institute for Physical and Theoretical Chemistry, Goethe-Universität Frankfurt, Max-von-Laue-Straße 8, D-60438 Frankfurt am Main, Germany.

Among the many in vitro-selected aptamers derived from SELEX protocols, only a small fraction has the potential to be applied for synthetic riboswitch engineering. Here, we present a comparative study of the binding properties of three different aptamers that bind to ciprofloxacin with similar KD values, yet only two of them can be applied as riboswitches. We used the inherent ligand fluorescence that is quenched upon binding as the reporter signal in fluorescence titration and in time-resolved stopped-flow experiments. Read More

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Enhancers are activated by p300/CBP activity-dependent PIC assembly, RNAPII recruitment, and pause release.

Mol Cell 2021 05 24;81(10):2166-2182.e6. Epub 2021 Mar 24.

Department of Proteomics, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, Copenhagen 2200, Denmark. Electronic address:

The metazoan-specific acetyltransferase p300/CBP is involved in activating signal-induced, enhancer-mediated transcription of cell-type-specific genes. However, the global kinetics and mechanisms of p300/CBP activity-dependent transcription activation remain poorly understood. We performed genome-wide, time-resolved analyses to show that enhancers and super-enhancers are dynamically activated through p300/CBP-catalyzed acetylation, deactivated by the opposing deacetylase activity, and kinetic acetylation directly contributes to maintaining cell identity at very rapid (minutes) timescales. Read More

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Analysis of multi-component broadband absorbers based on wavelength modulation united absorption spectroscopy.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Jul 9;255:119661. Epub 2021 Mar 9.

State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University, Beijing 100084, China. Electronic address:

Wavelength Modulation United Absorption Spectroscopy (WM-UAS), which is virtually an extension of Wavelength Modulation Spectroscopy (WMS), for measuring multi-component broadband absorbers is proposed and demonstrated in this paper. "Broadband absorbers" indicates matters that do not exhibit resolved absorption spectra within a certain wavelength range. WM-UAS requires the assistance of a kind of probe substance which exhibits a series of narrow absorption profiles to measure broadband absorbers. Read More

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[Sport and exercise in prevention and therapy of cardiovascular diseases].

Dtsch Med Wochenschr 2021 Mar 18;146(6):381-388. Epub 2021 Mar 18.

Sport and exercise improve physical performance, quality of life and prognosis in virtually all cardiovascular risk factors and diseases. Not only moderate endurance exercise but also specific strength training programs are recommended. However, research and practice are also focusing on more intense, interval exercise and targeted interventions to improve performance. Read More

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A Pipeline for Non-model Organisms for Transcriptome Assembly, Annotation, and Gene Ontology Analysis Using Open Tools: Case Study with Scots Pine.

Bio Protoc 2021 Feb 5;11(3):e3912. Epub 2021 Feb 5.

Russian Institute of Radiology and Agroecology, Obninsk, Russia.

RNA sequencing (RNA-seq) has opened up the possibility of studying virtually any organism at the whole transcriptome level. Nevertheless, the absence of a sequenced and accurately annotated reference genome may be an obstacle for applying this technique to non-model organisms, especially for those with a complex genome. While transcriptome assembly can circumvent this problem, it is often computationally demanding. Read More

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February 2021

Viroporins vs. Other Pore-Forming Proteins: What Lessons Can We Take?

Authors:
Eva Žerovnik

Front Chem 2021 18;9:626059. Epub 2021 Feb 18.

Department of Biochemistry and Molecular and Structural Biology, J. Stefan Institute, Ljubljana, Slovenia.

Pore-forming proteins (PFPs) exist in virtually all domains of life, and by disrupting cellular membranes, depending on the pore size, they cause ion dis-balance, small substances, or even protein efflux/influx, influencing cell's signaling routes and fate. Such pore-forming proteins exist from bacteria to viruses and also shape host defense systems, including innate immunity. There is strong evidence that amyloid toxicity is also caused by prefibrillar oligomers making "amyloid pores" into cellular membranes. Read More

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February 2021

Training During Social Distancing: The Effects of Remote Virtual Psychiatric Readiness Curriculum on Military Behavioral Health Providers.

Med J (Ft Sam Houst Tex) 2021 Jan-Mar(PB 8-21-01/02/03):3-7

staff psychiatrist at Tripler Army Medical Center.

There is inconsistency in the training of military medical providers on the regulations and procedures outlining US Army-specific psychiatric readiness related competencies. These competencies are necessary to ensure the appropriate categorization of a soldier's psychiatric readiness. There exists a need for a formal, comprehensive training curriculum accessible to all providers that is time- and cost-effective. Read More

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