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Quantification of training load distribution in mixed martial arts athletes: A lack of periodisation and load management.

PLoS One 2021 10;16(5):e0251266. Epub 2021 May 10.

Research Institute for Sport and Exercise Sciences, Liverpool John Moores University, Liverpool, United Kingdom.

The aim of this study was to quantify typical training load and periodisation practices of MMA athletes. MMA competitors (n = 14; age = 22.4 ± 4. Read More

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Learning From Clinical Consensus Diagnosis in India to Facilitate Automatic Classification of Dementia: Machine Learning Study.

JMIR Ment Health 2021 May 10;8(5):e27113. Epub 2021 May 10.

Center for Economic and Social Research, University of Southern California, Los Angeles, CA, United States.

Background: The Harmonized Diagnostic Assessment of Dementia for the Longitudinal Aging Study in India (LASI-DAD) is the first and only nationally representative study on late-life cognition and dementia in India (n=4096). LASI-DAD obtained clinical consensus diagnosis of dementia for a subsample of 2528 respondents.

Objective: This study develops a machine learning model that uses data from the clinical consensus diagnosis in LASI-DAD to support the classification of dementia status. Read More

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Prospective inference of bioprocess cell viability through chemometric modeling of fluorescence multiway data.

Biotechnol Prog 2021 May 10. Epub 2021 May 10.

Laboratorio de Desarrollo Analítico y Quimiometría (LADAQ), Cátedra de Química Analítica I, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Ciudad Universitaria, Santa Fe, Argentina.

In this investigation, the fermentation step of a standard mammalian cell-based industrial bioprocess for the production of a therapeutic protein was studied, with particular emphasis on the evolution of cell viability. This parameter constitutes one of the critical variables for bioprocess monitoring, since it can affect downstream operations and the quality of the final product. In addition, when the cells experiment an unpredictable drop in viability, the assessment of this variable through classic off-line methods may not provide information sufficiently in advance to take corrective actions. Read More

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Mapping spatial supply chain paths for embodied water flows driven by food demand in China.

Sci Total Environ 2021 May 3;786:147480. Epub 2021 May 3.

Key Laboratory for City Cluster Environmental Safety and Green Development of the Ministry of Education, Institute of Environmental and Ecological Engineering, Guangdong University of Technology, Guangzhou 510006, People's Republic of China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, People's Republic of China.

Identifying critical spatial supply chain paths for embodied water flows driven by food demand can guide the development of more spatially explicit food-related policies for water savings. Previous studies have quantified water uses caused by food demand, but overlook intermediate transfer paths within and among regions. That is, spatial supply chain paths describing step-by-step transfer stages between water uses and final food demand have not been well characterized. Read More

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Predicting the need for step-up therapy after EUS-guided drainage of pancreatic fluid collections with lumen-apposing metal stents.

Clin Gastroenterol Hepatol 2021 May 6. Epub 2021 May 6.

Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN.

Background & Aims: A significant proportion of individuals with pancreatic fluid collections (PFCs) require step-up therapy after endoscopic drainage with lumen-apposing metal stents (LAMS). The aim of this study is to identify factors associated with PFCs that require step-up therapy.

Methods: A retrospective cohort study of patients undergoing EUS-guided drainage of PFCs with LAMS from 4/2014 to 10/2019 at a single center was performed. Read More

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Live Observational Objective Assessment of Operative Performance in a Cadaveric Model is Equivalent to Delayed Video-Based Assessment.

Arthroscopy 2021 May 5. Epub 2021 May 5.

Faculty of Life and Health Sciences, Ulster University; Londonderry, UK Prof; Director of Research and Learning, ORSI Academy. Proefhoevestraat 12 | 9090 Melle, Belgium.

Purpose: The purpose of our study was to compare real time, live observational scoring with delayed retrospective video review of operative performance, and to determine if the evaluation method affected attainment of proficiency benchmarks.

Methods: Sixteen arthroscopy / sports medicine fellows and two senior residents completed training to perform arthroscopic Bankart (ABR) and rotator cuff repairs (ARCR) using a proficiency-based progression curriculum. Each final operative performance for 15 randomly selected ABRs and 13 ARCRs performed on cadavers were scored live (observation during the operative performance) and on delayed video review (6 - 8 weeks) by one of fifteen trained raters using validated metric-based (step and error) assessment tools. Read More

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What's the choice for goji: Lycium barbarum L. or L. chinense Mill.?

J Ethnopharmacol 2021 May 5:114185. Epub 2021 May 5.

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China. Electronic address:

Ethnopharmacological Relevance: For over one millennium, goji berries have been used traditionally as food and medicine in eastern Asia. In recent decades, it has become increasingly popular globally. However, the biocultural development of goji is poorly known. Read More

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Integration of peripheral transcriptomics, genomics, and interactomics following trauma identifies causal genes for symptoms of post-traumatic stress and major depression.

Mol Psychiatry 2021 May 7. Epub 2021 May 7.

Department of Psychiatry, Dell School of Medicine, Univerity of Texas, Austin, TX, USA.

Posttraumatic stress disorder (PTSD) is a debilitating syndrome with substantial morbidity and mortality that occurs in the aftermath of trauma. Symptoms of major depressive disorder (MDD) are also a frequent consequence of trauma exposure. Identifying novel risk markers in the immediate aftermath of trauma is a critical step for the identification of novel biological targets to understand mechanisms of pathophysiology and prevention, as well as the determination of patients most at risk who may benefit from immediate intervention. Read More

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Enhanced Recovery After Surgery strategies for elective craniotomy: a systematic review.

J Neurosurg 2021 May 7:1-25. Epub 2021 May 7.

1Machine Intelligence in Clinical Neuroscience (MICN) Laboratory, Department of Neurosurgery, Clinical Neuroscience Center, University Hospital Zurich, University of Zurich, Switzerland.

Objective: Enhanced Recovery After Surgery (ERAS) has led to a paradigm shift in perioperative care through multimodal interventions. Still, ERAS remains a relatively new concept in neurosurgery, and there is no summary of evidence on ERAS applications in cranial neurosurgery.

Methods: The authors systematically reviewed the literature using the PubMed/MEDLINE, Embase, Scopus, and Cochrane Library databases for ERAS protocols and elements. Read More

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Complete Digital Restoration: Implant-Supported Prosthesis Using Rapid Prototyping and a Model-Free Approach.

Compend Contin Educ Dent 2021 Apr;42(4):182-186

Private Practice in Dental Radiology, Heraklion Crete, Greece.

This report presents a completely digital workflow utilizing digital intraoral scanning, rapid prototyping techniques, and a monolithic final restoration. A complete fixed prosthesis supported by six implants was used to replace a patient's periodontally compromised mandibular natural dentition. A staged approach was used in which a series of milled and 3D-printed provisional restorations were fabricated to help the patient transition to the dental implant rehabilitation. Read More

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Phosphorylation of the WH2 domain in yeast Las17/WASP regulates G-actin binding and protein function during endocytosis.

Sci Rep 2021 May 6;11(1):9718. Epub 2021 May 6.

Department of Biomedical Science, Firth Court, University of Sheffield, Sheffield, S10 2TN, UK.

Actin nucleation is the key rate limiting step in the process of actin polymerization, and tight regulation of this process is critical to ensure actin filaments form only at specific times and at defined regions of the cell. WH2 domains are short sequence motifs found in many different actin binding proteins including WASP family proteins which regulate the actin nucleating complex Arp2/3. In this study we reveal a phosphorylation site, Serine 554, within the WH2 domain of the yeast WASP homologue Las17. Read More

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Type 2 and type 3 innate lymphoid cells at the maternal-fetal interface: implications in preterm birth.

BMC Immunol 2021 May 6;22(1):28. Epub 2021 May 6.

Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research (iCBR), University of Coimbra, Polo III - Health Sciences Campus, Azinhaga Santa Comba, Celas, 3000-548, Coimbra, Portugal.

Background: Preterm birth (PTB) is one of the major causes of neonatal morbidity and mortality worldwide. It is commonly accepted that the act of giving birth is the final step in a proinflammatory signaling cascade, orchestrated by an intrauterine milieu coupled to hormonal cues. Consequently, the inflammatory process plays a pivotal role during the pathogenesis of human labor, both in term and preterm deliveries. Read More

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Neonicotinoids stimulate H-limited methane emission in Periplaneta americana through the regulation of gut bacterium community.

Environ Pollut 2021 Apr 27;285:117237. Epub 2021 Apr 27.

Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, China. Electronic address:

Methane emitted by insects is considered to be an important source of atmospheric methane. Here we report the stimulation of methane emission in the cockroach Periplaneta americana and termite Coptotermes chaohuensis, insects with abundant methanogens, by neonicotinoids, insecticides widely used to control insect pests. Cycloxaprid (CYC) and imidacloprid (IMI) caused foregut expansion in P. Read More

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Large scale purification and characterization of A21 deamidated variant-most prominent post translational modification (PTM) for insulins which is also widely observed in insulins pharmaceutical manufacturing and storage.

Protein Expr Purif 2021 May 3;185:105895. Epub 2021 May 3.

Biocon Biologics Limited (BBL), Research and Development (RND), Biocon Park, Bommasandra Jigani Link Road, Bangalore, 560 099, India. Electronic address:

Biopharmaceutical development demands appropriate understanding of product related variants, which are formed due to post-translational modification and during downstream processing. These variants can lead to low yield, reduced biological activity, and suboptimal product quality. In addition, these variants may undergo immune reactions, henceforth need to be appropriately controlled to ensure consistent product quality and patient safety. Read More

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Domain adaptation and self-supervised learning for surgical margin detection.

Int J Comput Assist Radiol Surg 2021 May 6. Epub 2021 May 6.

School of Computing, Queen's University, Ontario, Canada.

Purpose: One in five women who undergo breast conserving surgery will need a second revision surgery due to remaining tumor. The iKnife is a mass spectrometry modality that produces real-time margin information based on the metabolite signatures in surgical smoke. Using this modality and real-time tissue classification, surgeons could remove all cancerous tissue during the initial surgery, improving many facets of patient outcomes. Read More

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Decision-making framework for an acute care clinical pharmacist productivity model: Part 1.

Am J Health Syst Pharm 2021 May 4. Epub 2021 May 4.

University of North Carolina Medical Center, Chapel Hill, NC, USA.

Disclaimer: In an effort to expedite the publication of articles related to the COVID-19 pandemic, AJHP is posting these manuscripts online as soon as possible after acceptance. Accepted manuscripts have been peer-reviewed and copyedited, but are posted online before technical formatting and author proofing. These manuscripts are not the final version of record and will be replaced with the final article (formatted per AJHP style and proofed by the authors) at a later time. Read More

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Enrichment of Palmitoleic Acid by a Combination of Two-step Solvent Crystallization and Molecular Distillation.

J Oleo Sci 2021 ;70(5):599-606

State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University.

Palmitoleic acid shows a variety of beneficial properties to human health. In this study, enrichment of palmitoleic acid from sea buckthorn pulp oil by two-step solvent crystallization and molecular distillation was investigated. Sea buckthorn pulp oil was first converted to its corresponding mixed fatty acids (SPOMFs) containing 27. Read More

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January 2021

Performance-based balance tests, combined with the number of falls recalled in the past year, predicts the incidence of future falls in established unilateral transtibial prosthesis users.

PM R 2021 May 5. Epub 2021 May 5.

Department of Rehabilitation Medicine, University of Washington, Seattle, WA, 98195.

Introduction: Falls are common and consequential events for lower limb prosthesis (LLP) users. Currently, there are no models based on prospective falls data that clinicians can use to predict the incidence of future falls in LLP users. Assessing who is at risk for falls, and thus most likely to need and benefit from intervention, remains a challenge. Read More

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Mechanistic studies of Cp*Ir(III)/Cp*Rh(III)-catalyzed branch-selective allylic C-H amidation: why is Cp*Ir(III) superior to Cp*Rh(III)?

Org Biomol Chem 2021 May;19(17):3850-3858

Department of Chemistry, School of Science, Tianjin University, Tianjin 300072, China.

Density functional theory calculations have revealed the mechanism and origins of the reactivity and regioselectivity of the Cp*Ir(iii)/Cp*Rh(iii)-catalyzed allylic C-H amidation of alkenes and dioxazolones. Generally, the catalytic cycle consists of alkene coordination, C(sp3)-H activation, dioxazolone oxidative addition, reductive elimination and proto-demetallation to give the final amidation product. The C-H activation is found to be the rate-determining step, and it controls the reactivity of the reaction. Read More

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Subpixel Matching Using Double-Precision Gradient-Based Method for Digital Image Correlation.

Sensors (Basel) 2021 Apr 30;21(9). Epub 2021 Apr 30.

School of Civil Engineering, Chongqing University, No. 83 Shabei Street, Chongqing 400045, China.

Digital image correlation (DIC) for displacement and strain measurement has flourished in recent years. There are integer pixel and subpixel matching steps to extract displacement from a series of images in the DIC approach, and identification accuracy mainly depends on the latter step. A subpixel displacement matching method, named the double-precision gradient-based algorithm (DPG), is proposed in this study. Read More

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Fetal QRS Detection in Noninvasive Abdominal Electrocardiograms Using Principal Component Analysis and Discrete Wavelet Transforms with Signal Quality Estimation.

J Biomed Phys Eng 2021 Apr 1;11(2):197-204. Epub 2021 Apr 1.

PhD, Department of Mechanical Engineering, K. N. Toosi University of Technology, Tehran, Iran.

Background: Fetal heart rate (FHR) extracted from abdominal electrocardiogram (ECG) is a powerful non-invasive method in appropriately assessing the fetus well-being during pregnancy. Despite significant advances in the field of electrocardiography, the analysis of fetal ECG (FECG) signal is considered a challenging issue which is mainly due to low signal to noise ratio (SNR) of FECG.

Objective: In this study, we present an approach for accurately locating the fetal QRS complexes in non-invasive FECG. Read More

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Speeding up Hartree-Fock and Kohn-Sham calculations with first-order corrections.

J Chem Phys 2021 Apr;154(16):164114

Department of Physical Chemistry and Materials Science, Budapest University of Technology and Economics, P.O. Box 91, H-1521 Budapest, Hungary.

Several approaches are presented to improve the efficiency of Hartree-Fock and Kohn-Sham self-consistent field (SCF) calculations relying on a simple first-order energy correction reminiscent of the scheme used in dual-basis SCF methods. The basic idea is to perform an initial SCF calculation computing approximate Fock-matrices and, in the final iteration step, to use a more complete Fock-matrix builder together with the energy correction to diminish the error. The approximation is tested for conventional and local density fitting (DF) SCF approaches combining various auxiliary basis sets, fitting metrics, and Fock-matrix construction algorithms in the initial and final iterations as well as for seminumerical SCF methods combining integration grids of different qualities. Read More

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The process of methanogenesis in paddy fields under different elevated CO concentrations.

Sci Total Environ 2021 Jun 5;773:145629. Epub 2021 Feb 5.

Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters, Jiangsu Key Laboratory of Agricultural Meteorology, School of Applied Meteorology, Nanjing University of Information Science & Technology, Nanjing 210044, China.

Understanding the process of methanogenesis in paddy fields under the scenarios of future climate change is of great significance for reducing greenhouse gas emissions and regulating the soil carbon cycle. Methyl Coenzyme M Reductase subunit A (mcrA) of methanogens is a rate-limiting enzyme that catalyzes the final step of CH production. However, the mechanism of methanogenesis change in the paddy fields under different elevated CO concentrations (e[CO]) is rarely explored in earlier studies. Read More

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Pragmatic randomized controlled trial for stepping down of asthma controller treatment in patients controlled with low dose inhaled corticosteroid and long-acting beta2 agonist: SIGMA study.

J Allergy Clin Immunol Pract 2021 Apr 30. Epub 2021 Apr 30.

Department of Allergy and Clinical Immunology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea,. Electronic address:

Background: Current asthma guidelines recommend stepping down of controller treatment when the condition is well-controlled for a certain length of time. However, an optimal stepping-down strategy for well-controlled patients receiving a low-dose inhaled corticosteroid (ICS) with a long-acting β2 agonist (LABA) remains unclear.

Objective: This study was a randomized, open-label, 3-arm parallel pragmatic trial comparing two kinds of step-down approaches for maintaining treatment. Read More

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Ability of different matrices to transmit African swine fever virus.

EFSA J 2021 Apr 27;19(4):e06558. Epub 2021 Apr 27.

This opinion assesses the risk posed by different matrices to introduce African swine fever virus (ASFV) to non-affected regions of the EU. Matrices assessed are feed materials, enrichment/bedding materials and empty live pigs transport vehicles returning from affected areas. Although the risk from feed is considered to be lower than several other pathways (e. Read More

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CTP Synthase 2 From Is Required for Complete Embryo Development.

Front Plant Sci 2021 15;12:652434. Epub 2021 Apr 15.

Plant Physiology, University of Kaiserslautern, Kaiserslautern, Germany.

Pyrimidine synthesis is an essential pathway in all organisms. The final and rate-limiting step in the synthesis of the nucleotide cytidine triphosphate (CTP) is catalyzed by CTP synthase (CTPS), and harbors five isoforms. Single mutant lines defective in each one of the four isoforms do not show apparent phenotypical alterations in comparison to wild-type plants. Read More

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E3 Ubiquitin Ligase in Anticancer Drugdsla Resistance: Recent Advances and Future Potential.

Front Pharmacol 2021 15;12:645864. Epub 2021 Apr 15.

Department of Thoracic Surgery, Xiangya Hospital, Central South University, Changsha, China.

Drug therapy is the primary treatment for patients with advanced cancer. The use of anticancer drugs will inevitably lead to drug resistance, which manifests as tumor recurrence. Overcoming chemoresistance may enable cancer patients to have better therapeutic effects. Read More

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Microbial methionine transporters and biotechnological applications.

Appl Microbiol Biotechnol 2021 Apr 30. Epub 2021 Apr 30.

Australian Regenerative Medicine Institute (ARMI), Faculty of Medicine, Nursing and Health Sciences, Monash University, Melbourne, Clayton, Australia.

Methionine (Met) is an essential amino acid with commercial value in animal feed, human nutrition, and as a chemical precursor. Microbial production of Met has seen intensive investigation towards a more sustainable alternative to the chemical synthesis that currently meets the global Met demand. Indeed, efficient Met biosynthesis has been achieved in genetically modified bacteria that harbor engineered enzymes and streamlined metabolic pathways. Read More

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Patient-Reported Outcome-Based Quality Indicators in Dutch Primary Care Physical Therapy for Patients With Nonspecific Low Back Pain: A Cohort Study.

Phys Ther 2021 Apr 30. Epub 2021 Apr 30.

Radboud university medical center, Radboud Institute for Health Sciences, IQ healthcare, PO Box 9101, 6500 HB, Nijmegen, Netherlands.

Objective: The purpose of this study was to define and select a core set of outcome-based quality indicators, accepted by stakeholders on usability and perceived added value as a quality improvement tool, and to formulate recommendations for the next implementation step.

Methods: In phase 1, we defined 15 potential quality indicators for patient-reported outcome measures (PROMs) and associated domains, namely the Numeric Pain Rating Scale (NPRS) for pain intensity, the Patient Specific Functioning Scale (PSFS) for physical activity, the Quebec Back Pain Disability Scale (QBPDS) for physical functioning, and the Global Perceived Effect - Dutch Version (GPE-DV) for perceived effect. We described their comparability and discriminatory characteristics using cohort data. Read More

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AKT ISOFORMS-AS160-GLUT4: The defining axis of insulin resistance.

Rev Endocr Metab Disord 2021 Apr 30. Epub 2021 Apr 30.

Kusuma School of Biological Sciences, Indian Institute of Technology-Delhi, Hauz Khas, New Delhi, 110016, India.

The Akt isoforms-AS160-GLUT4 axis is the primary axis that governs glucose homeostasis in the body. The first step on the path to insulin resistance is deregulated Akt isoforms. This could be Akt isoform expression, its phosphorylation, or improper isoform-specific redistribution to the plasma membrane in a specific tissue system. Read More

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