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Genetic Diversity and Population Structure of the Xanthomonas campestris pv. campestris Strains Affecting Cabbages in China Revealed by MLST and Rep-PCR Based Genotyping.

Plant Pathol J 2021 Oct 1;37(5):476-488. Epub 2021 Oct 1.

Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot for cruciferous vegetables worldwide, especially for the cole crops such as cabbage and cauliflower. Due to the lack of resistant cabbage cultivars, black rot has brought about considerable yield losses in recent years in China. Read More

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October 2021

Interfacial behaviour and quantitative analysis of hexadecyl phosphocholine drug at a polarized liquid/liquid interface.

Analyst 2021 Nov 30. Epub 2021 Nov 30.

Universidad Nacional de Córdoba, Facultad de Ciencias Químicas, Departamento de Fisicoquímica, Córdoba, Argentina.

The interfacial behaviour of the amphiphilic drug hexadecyl phosphocholine (HePC, also called miltefosine) was analysed by cyclic voltammetry applied at the water/1,2-dichloroethane interface. HePC is the only oral drug currently approved for the treatment of visceral, mucosal  and cutaneous leishmaniasis. Because of its amphiphilic character, it can interact with biological membranes, solubilizing their compounds and leading to cell disruption. Read More

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November 2021

Vascular ageing and peripheral pulse: an improved model for assessing their relationship.

Physiol Meas 2021 Nov 30. Epub 2021 Nov 30.

Dept. of Information Engineering, Universita di Firenze, Via S Marta 3, 50139 Firenze, Firenze, ITALY.

Objective: Vascular ageing is associated with several alterations, including arterial stiffness and endothelial dysfunction. Such alterations represent an independent factor in the development of cardiovascular disease. In our previous works we demonstrated the alterations occurring in the vascular system are themselves reflected in the shape of the peripheral waveform; thus, a model that describes the waveform as a sum of Gaussian curves provides a set of parameters that successfully discriminate between under(<= 35 years old) and over subjects (> 35 years old). Read More

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November 2021

UPLC-MS/MS method for the simultaneous quantification of caffeine and illicit psychoactive drugs in hair using a single-step high-speed grinding extraction - Insights into a cut-off value for caffeine abuse.

J Pharm Biomed Anal 2021 Nov 25;209:114489. Epub 2021 Nov 25.

School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China; China National Narcotics Control Commission - China Pharmaceutical University Joint Laboratory on Key Technologies of Narcotics Control, Nanjing 210009, China. Electronic address:

Caffeine is a commonly consumed psychoactive substance whose addictive potential has long been reported. Excessive caffeine intake may lead to severe health damage or drug addiction problems; however, studies on the surveillance of caffeine abuse by the Chinese population are lacking. This study aimed to propose a concentration value for caffeine based on hair analysis to distinguish excessive intake from normal consumption, and provide an analytical tool for forensic toxicology investigations of caffeine and other frequently abused drugs. Read More

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November 2021

F-FDG PET/MR in focal epilepsy: A new step for improving the detection of epileptogenic lesions.

Epilepsy Res 2021 Nov 20;178:106819. Epub 2021 Nov 20.

Université Paris-Saclay, CEA, CNRS, Inserm, Biomaps, Service Hospitalier Frédéric Joliot, 4 place du général Leclerc, 91401 ORSAY France; Department of Neurosurgery, Epilepsy Unit, Groupe Hospitalier Universitaire Psychiatrie et Neurosciences, Sainte-Anne Hospital, 1 rue Cabanis, 75014 Paris, France. Electronic address:

Purpose: Hybrid PET/MR is a promising tool in focal drug-resistant epilepsy, however the additional value for the detection of epileptogenic lesions and surgical decision-making remains to be established.

Methods: We retrospectively compared F-FDG PET/MR images with those obtained by a previous F-FDG PET co-registered with MRI (PET+MR) in 25 consecutive patients (16 females, 13-60 years) investigated for focal drug-resistant epilepsy. Visual analysis was performed by two readers blinded from imaging modalities, asked to assess the technical characteristics (co-registration, quality of images), the confidence in results, the location of PET abnormalities and the presence of a structural lesion on MRI. Read More

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November 2021

Investigating the underlying biomechanical mechanisms leading to falls in long-term ankle-foot orthosis and functional electrical stimulator users with chronic stroke.

Gait Posture 2021 Nov 20;92:144-152. Epub 2021 Nov 20.

School of Biological and Health System Engineering, Arizona State University, Tempe, AZ, USA. Electronic address:

Background: Ankle-foot-orthoses (AFOs) and functional electrical stimulators (FES) are commonly prescribed to treat foot-drop in individuals with stroke. Despite well-established positive impacts of AFO and FES devices on balance and gait, AFO and FES-users still fall at a high rate.

Objective: The objective of this study was to investigate 1) the underlying biomechanical mechanisms leading to a fall in long-term AFO and FES-users with chronic stroke and 2) the impacts of AFOs and FES devices on fall outcomes and compensatory stepping response of long-term users with chronic stroke. Read More

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November 2021

Microbial regulation of hexokinase 2 links mitochondrial metabolism and cell death in colitis.

Cell Metab 2021 Nov 20. Epub 2021 Nov 20.

Institute of Clinical Molecular Biology, University of Kiel, 24105 Kiel, Germany. Electronic address:

Hexokinases (HK) catalyze the first step of glycolysis limiting its pace. HK2 is highly expressed in gut epithelium, contributes to immune responses, and is upregulated during inflammation. We examined the microbial regulation of HK2 and its impact on inflammation using mice lacking HK2 in intestinal epithelial cells (Hk2). Read More

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November 2021

Disproportionation of the Uranyl(V) Coordination Complexes in Aqueous Solution through Outer-Sphere Electron Transfer.

Inorg Chem 2021 Nov 30. Epub 2021 Nov 30.

Department of Chemistry, School of Natural Sciences, The University of Manchester, Oxford Road, Manchester M13 9PL, U.K.

Among the linear actinyl(VI/V) cations, the uranyl(V) species are particularly intriguing because they are unstable and exhibit a unique behavior to undergo H promoted disproportionation in aqueous solution and form stable uranyl(VI) and U(IV) complexes. This study uses density functional theory (DFT) combined with the conductor-like polarizable continuum model approach to investigate [UO] to [UO] reduction free energies (RFEs) and explores the stability of uranyl(V) complexes in aqueous solution through computing disproportionation free energies (DFEs) for an outer-sphere electron transfer process. In addition to the aqua complex (U1), another three commonly encountered ligands such as chloride (U2), acetate (U3), and carbonate (U4) in aqueous environmental conditions are taken into account. Read More

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November 2021

Aptamer-Functionalized Nanochannels for One-Step Detection of SARS-CoV-2 in Samples from COVID-19 Patients.

Anal Chem 2021 Nov 30. Epub 2021 Nov 30.

State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 210023 Nanjing, P. R. China.

With the outbreak of COVID-19, which is fast transmitting and highly contagious, the development of rapid, highly specific, and sensitive detection kits has become a research hotspot. The existing assay methods for SARS-CoV-2 are mainly based on enzymatic reactions, which require expensive reagents, hindering popular use, especially in resource-constrained areas. Herein, we propose an aptamer-based method for the assay of SARS-CoV-2 via binding of the spike protein using functionalized biomimetic nanochannels. Read More

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November 2021

Tuning Metal-Support Interaction of Pt-CeO Catalysts for Enhanced Oxidation Reactivity.

Environ Sci Technol 2021 Nov 30. Epub 2021 Nov 30.

State Key Joint Laboratory of Environment Simulation and Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.

Metal-support interaction (MSI) has been widely recognized to be playing a pivotal role in regulating the catalytic activity of various reactions. In this work, the degree of MSI between Pt and CeO support was finely tuned by adjusting the activation condition, and the obtained catalysts were tested for the oxidative abatement of CO and HCHO under ambient conditions. The characterization of catalysts shows that activation of strongly interacting Pt-CeO at higher temperatures by H leads to a weaker MSI with increased electron density of Pt, and this modification of local electronic properties is demonstrated to result in enhanced O adsorption/activation to prevent the CO self-poisoning effect, while it abates the activity of CO adsorption/activation and oxidation of adsorbed CO. Read More

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November 2021

Cost-effectiveness analysis of hepatitis C virus (HCV) point-of-care assay for HCV screening.

Liver Int 2021 Nov 30. Epub 2021 Nov 30.

University of Waterloo, School of Pharmacy, Waterloo, ON, Canada.

Background/aim: Hepatitis C virus (HCV) continues to pose significant public health concerns with approximately 44% of chronically infected Canadians undiagnosed. The current HCV screening in Canada is a two-step diagnosis pathway consisting of anti-HCV testing and HCV ribonucleic acid (RNA) testing. The introduction of HCV point-of-care assays, such as the Xpert HCV viral load finger-stick assay, can facilitate HCV RNA diagnosis during a single visit and provide quick linkage to care. Read More

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November 2021

A step back in time: The basics of CF care still matter!

Pediatr Pulmonol 2021 Nov 30. Epub 2021 Nov 30.

Cystic Fibrosis Foundation, Bethesda, Maryland, USA.

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November 2021

Sequence-dependent twist-bend coupling in DNA minicircles.

Nanoscale 2021 Nov 30. Epub 2021 Nov 30.

Department of Chemistry and Nanoscience, Ewha Womans University, Seoul 03760, Republic of Korea.

Looping of double-stranded DNA molecules with 100-200 base pairs into minicircles, catenanes, and rotaxanes has been suggested as a potential tool for DNA nanotechnologies. However, sharp DNA bending into a minicircle with a diameter of several to ten nanometers occurs with alterations in the DNA helical structure and may lead to defective kink formation that hampers the use of DNA minicircles, catenanes, and rotaxanes in nanoscale DNA applications. Here, we investigated local variations of a helical twist in sharply bent DNA using microsecond-long all-atom molecular dynamics simulations of six different DNA minicircles, focusing on the sequence dependence of the coupling between DNA bending and its helical twist. Read More

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November 2021

Synthesis of illudalic acid and analogous phosphatase inhibitors.

Org Biomol Chem 2021 Nov 30. Epub 2021 Nov 30.

C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, 26506, USA.

Developing an efficient, concise synthesis of the fungal natural product illudalic acid has been a long-standing challenge, made more pressing by the recent discovery that illudalic acid and analogs are selective phosphatase inhibitors. Syntheses of illudalic acid have become progressively more efficient over the decades yet remain strategically grounded in a 17-step synthesis reported in 1977. Here we validate a two-step process-convergent [4 + 2] benzannulation and one-pot coordinated functional group manipulations-for preparing the key trifunctional pharmacophore of illudalic acid. Read More

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November 2021

Application Scenarios for Artificial Intelligence in Nursing Care: Rapid Review.

J Med Internet Res 2021 Nov 29;23(11):e26522. Epub 2021 Nov 29.

Institute of Public Health and Nursing Research, High Profile Area Health Sciences, University of Bremen, Bremen, Germany.

Background: Artificial intelligence (AI) holds the promise of supporting nurses' clinical decision-making in complex care situations or conducting tasks that are remote from direct patient interaction, such as documentation processes. There has been an increase in the research and development of AI applications for nursing care, but there is a persistent lack of an extensive overview covering the evidence base for promising application scenarios.

Objective: This study synthesizes literature on application scenarios for AI in nursing care settings as well as highlights adjacent aspects in the ethical, legal, and social discourse surrounding the application of AI in nursing care. Read More

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November 2021

Design and Modeling of Bioreactor Utilizing Electrophoresis and Di-electrophoresis Techniques for Regenerating Reabsorption Function of Human Kidney PCT in Microfluidics Environment.

IEEE Trans Nanobioscience 2021 Nov 30;PP. Epub 2021 Nov 30.

The need for innovation in medical device technology is immense; especially to replace the dialysis techniques the necessity is extremely high. The available techniques that promised to replace dialysis have not yet geared up to the marketization level. The utilization of live kidney cells makes these devices costly, delicate, and unreliable. Read More

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November 2021

CNN-Based Image Reconstruction Method for Ultrafast Ultrasound Imaging.

IEEE Trans Ultrason Ferroelectr Freq Control 2021 Nov 30;PP. Epub 2021 Nov 30.

Ultrafast ultrasound (US) revolutionized biomedical imaging with its capability of acquiring full-view frames at over 1 kHz, unlocking breakthrough modalities such as shear-wave elastography and functional US neuroimaging. Yet, it suffers from strong diffraction artifacts, mainly caused by grating lobes, side lobes, or edge waves. Multiple acquisitions are typically required to obtain a sufficient image quality, at the cost of a reduced frame rate. Read More

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November 2021

Anaerobic oxidation of methane coupled with sulphate reduction: high concentration of methanotrophic archaea might be responsible for low stable isotope fractionation factors in methane.

Isotopes Environ Health Stud 2021 Nov 30:1-16. Epub 2021 Nov 30.

Water Problems Institute, Russian Academy of Sciences, Moscow, Russian Federation.

The changes in CH and CHH during sulphate-dependent anaerobic oxidation of methane (AOM) were described using dynamic modelling. The batch sulphate-dependent AOM at the nearly linear dynamics of methane oxidation with different enriched cultures originating from three marine sediments was simulated. The traditional Rayleigh equation for carbon and hydrogen stable isotopes in methane was derived from the basic dynamic isotope equation. Read More

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November 2021

A Reactivity-Based F-Labeled Probe for PET Imaging of Oxidative Stress in Chemotherapy-Induced Cardiotoxicity.

Mol Pharm 2021 Nov 30. Epub 2021 Nov 30.

School of Biomedical Engineering & Imaging Sciences, King's College London, King's Health Partners, St Thomas' Hospital, London SE1 7EH, United Kingdom.

Oxidative stress underlies the pathology of many human diseases, including the doxorubicin-induced off-target cardiotoxicity in cancer chemotherapies. Since current diagnostic procedures are only capable of monitoring cardiac function, a noninvasive means of detecting biochemical changes in redox status prior to irreversible functional changes is highly desirable for both early diagnosis and prognosis. We designed a novel F-labeled molecular probe, F-FPBT, for the direct detection of superoxide using positron emission tomography (PET). Read More

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November 2021

Scalable Fabrication and Use of 3D Structured Microparticles Spatially Functionalized with Biomolecules.

ACS Nano 2021 Nov 30. Epub 2021 Nov 30.

Department of Bioengineering, University of California - Los Angeles, Los Angeles, California 90095, United States.

Microparticles with defined shapes and spatial chemical modification can interface with cells and tissues at the cellular scale. However, conventional methods to fabricate shaped microparticles have trade-offs between the throughput of manufacture and the precision of particle shape and chemical functionalization. Here, we achieved scalable production of hydrogel microparticles at rates of greater than 40 million/hour with localized surface chemistry using a parallelized step emulsification device and temperature-induced phase-separation. Read More

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November 2021

Transforming CO into Methanol with N-Heterocyclic Carbene-Stabilized Coinage Metal Hydrides Immobilized in a Metal-Organic Framework UiO-68.

ACS Appl Mater Interfaces 2021 Nov 30. Epub 2021 Nov 30.

Department of Chemical and Biomolecular Engineering, National University of Singapore 117576, Singapore.

By synergizing the advantages of homogeneous and heterogeneous catalysis, single-site heterogeneous catalysis represents a highly promising opportunity for many catalytic processes. Particularly, the unprecedented designability and versatility of metal-organic frameworks (MOFs) promote them as salient platforms for designing single-site catalytic materials by introducing isolated, well-defined active sites into the frameworks. Herein, we design new MOF-supported single-site catalysts for CO hydrogenation to methanol (CHOH), a reaction of great significance in CO valorization. Read More

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November 2021

Preparation of Practical High-Performance Electrodes for Acidic and Alkaline Media Water Electrolysis.

ChemSusChem 2021 Nov 30. Epub 2021 Nov 30.

Max-Planck-Institut für Kohlenforschung: Max-Planck-Institut fur Kohlenforschung, Heterogeneous Catalysis, Kaiser-Wilhelm-Platz 1, 45470, Mülheim an der Ruhr, GERMANY.

We merge the synthesis of electrocatalyst and the electrode preparation into one-step process, and report a versatile method to synthesize metal oxide electrocatalysts on the conductive carbon paper (CP). Very simply, the metal precursor deposited on the CP was thermally treated by a torch-gun for just 6 s, resulting in the formation of RuO 2 , Co 3 O 4 and mixed oxide nanoparticles. This can be directly used as working electrode for oxygen evolution reaction (OER). Read More

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November 2021

Rates and risk factors associated with 30- and 90-day readmissions and reoperations after spinal fusions for adult lumbar degenerative pathology and spinal deformity.

Spine Deform 2021 Nov 30. Epub 2021 Nov 30.

Department of Orthopaedic Surgery, University of California-San Francisco (UCSF), 500 Parnassus Avenue, MU West 3rd Floor Rm 321, San Francisco, CA, USA.

Purpose: Analyze state databases to determine variables associated with of short-term readmissions and reoperations following thoracolumbar spine fusions for degenerative pathology and spinal deformity.

Methods: Retrospective study of State Inpatient Database (2005-13, CA, NE, NY, FL, NC, UT).

Inclusion Criteria: age > 45 years, diagnosis of degenerative spinal deformity, ≥ 3 level posterolateral lumbar spine fusion. Read More

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November 2021

Augmenting photosynthesis through facile AIEgen-chloroplast conjugation and efficient solar energy utilization.

Mater Horiz 2021 May 26;8(5):1433-1438. Epub 2021 Apr 26.

Department of Chemical and Biological Engineering, Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Division of Life Science and Institute of Advanced Study, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.

Photosynthesis is regarded as the foundation for sustaining life on our planet. Light-harvesting is the initial step that activates the subsequent photochemical reactions. In the photosystems, chloroplast is the basic light-driven metabolic factory of higher plant cells. Read More

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Liquid-liquid interfaces: a unique and advantageous environment to prepare and process thin films of complex materials.

Authors:
Aldo J G Zarbin

Mater Horiz 2021 May 29;8(5):1409-1432. Epub 2021 Jan 29.

Departamento de Química, Universidade Federal do Paraná (UFPR), CP 19032, CEP 81531-980, Curitiba, PR, Brazil.

Thin film technology is pervasive for many fields with high impact in our daily lives, which makes processing materials such as thin films a very important subject in materials science and technology. However, several paramount materials cannot be prepared as thin films through the well-known and consolidated deposition routes, which strongly limits their applicability. This is particularly noticeable for multi-component and complex nanocomposites, which present unique properties due to the synergic effect between the components, but have several limitations to be obtained as thin films, mainly if homogeneity and transparence are required. Read More

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Direct formation of interlayer exciton in two-dimensional van der Waals heterostructures.

Mater Horiz 2021 Aug 15;8(8):2208-2215. Epub 2021 Jun 15.

New Energy Technology Engineering Laboratory of Jiangsu Province & School of Science, Nanjing University of Posts and Telecommunications, Nanjing 210023, China.

In atomically thin two-dimensional van der Waals (2D vdW) heterostructures, spatially separated interlayer excitons play an important role in the optoelectronic performance and show great potential for the exploration of many-body quantum phenomena. A commonly accepted formation mode for interlayer excitons is via a two-step intralayer exciton transfer mechanism, namely, photo-excited intralayer excitons are initially generated in individual sublayers, and photogenerated electrons and holes are then separated into opposite sublayers based on the type-II band alignment. Herein, we expand the concept of interlayer exciton formation and reveal that bright interlayer excitons can be generated in one step by direct interlayer photoexcitation in 2D vdW heterostructures that have strong interlayer coupling and a short photoexcitation channel. Read More

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One Small Step for Rhode Island Medicaid, One Giant Leap Towards Hepatitis C Elimination.

Authors:
Lynn E Taylor

R I Med J (2013) 2021 Dec 1;104(10):48-52. Epub 2021 Dec 1.

Director of HIV and Viral Hepatitis Services, CODAC Behavioral Health; Research Professor, University of Rhode Island; Director, RI Defeats Hep C, http://www.ridefeatshepc.com.

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December 2021

Characterizing the Tapering Practices of United States and Canadian Raw Powerlifters.

J Strength Cond Res 2021 Dec;35(Suppl 2):S26-S35

Exercise and Sport Sciences Laboratory, Center of Excellence for Sport Science and Coach Education, Department of Sport, Exercise, Recreation, and Kinesiology, East Tennessee State University, Johnson City, Tennessee.

Abstract: Travis, SK, Pritchard, HJ, Mujika, I, Gentles, JA, Stone, MH, and Bazyler, CD. Characterizing the tapering practices of United States and Canadian raw powerlifters. J Strength Cond Res 35(12S): S26-S35, 2021-The purpose of this study was to characterize the tapering practices used by North American powerlifters. Read More

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December 2021

The Future of Integrative Health: What Case Managers Need to Know?

Prof Case Manag 2022 Jan-Feb 01;27(1):39-43

Siobhan Hutchinson, MA, created Next Step Strategies, LLC, to educate and encourage individuals to discover their body's natural healing ability. Her clients report experiencing increased range of motion, balance, relaxation, reduced stress, and an overall sense of well-being. Siobhan received her master's degree in Holistic Health from Georgian Court University and is an accredited T'ai Chi Chih, Seijaku, Guigen Qigong, Reiki Master/Teacher, Medicinal Aromatherapist, and Certified Clinical Eden Energy Medicine Practitioner. The United Fellowship of Martial Artists awarded her Holistic Healing Artist & Qigong Master. She has studied in China and continues her studies with a variety of holistic practitioners. Her passion is stress relief and walking in serenity.

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November 2021

Perceptions of USMLE Step 1 Pass/Fail Score Reporting Among Orthopedic Surgery Residency Program Directors.

Orthopedics 2021 Dec 2:1-5. Epub 2021 Dec 2.

The United States Medical Licensing Examination (USMLE) Step 1 examination will transition from graded to pass/fail scoring starting no earlier than January 2022. Orthopedic surgery residency programs will need to adapt to these changes. The goal of this study was to investigate the perceptions of orthopedic surgery residency program directors on the change of Step 1 from a graded to a pass/fail examination. Read More

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December 2021