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Effectiveness of Chest CT in Children: CT Findings in Relation to the Clinical Question.

Rofo 2021 Oct 14. Epub 2021 Oct 14.

Diagnostic and Interventional Radiology, Universitätsklinikum Tübingen, Tübingen, Germany.

Purpose:  To estimate the effectiveness and efficiency of chest CT in children based on the suspected diagnosis in relation to the number of positive, negative, and inconclusive CT results.

Materials And Methods:  In this monocentric retrospective study at a university hospital with a division of pediatric radiology, 2019 chest CT examinations (973 patients; median age: 10.5 years; range: 2 days to 17. Read More

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October 2021

Pulmonary Imaging of Immunocompromised Patients during Hematopoietic Stem Cell Transplantation using Non-Contrast-Enhanced Three-Dimensional Ultrashort Echo Time (3D-UTE) MRI.

Rofo 2021 Oct 14. Epub 2021 Oct 14.

Department of Diagnostic and Interventional Radiology, University Hospital of Würzburg, Germany.

Purpose:  To evaluate the feasibility of non-contrast-enhanced three-dimensional ultrashort echo time (3D-UTE) MRI for pulmonary imaging in immunocompromised patients during hematopoietic stem cell transplantation (HSCT).

Methods:  MRI was performed using a stack-of-spirals 3D-UTE sequence (slice thickness: 2.34mm; matrix: 256 × 256; acquisition time: 12. Read More

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October 2021

INCB84344-201: Ponatinib and steroids in frontline therapy of unfit patients with Ph+ acute lymphoblastic leukemia.

Blood Adv 2021 Oct 14. Epub 2021 Oct 14.

Incyte Corporation, Wilmington, Delaware, United States.

Tyrosine kinase inhibitors have improved survival for patients with Philadelphia chromosome-positive (Ph+) acute lymphoblastic leukemia (ALL). However, prognosis for old or unfit patients remains poor. In the INCB84344-201 (formerly GIMEMA LAL 1811) prospective, multicenter, phase 2 trial, we tested the efficacy and safety of ponatinib plus prednisone in newly diagnosed patients with Ph+ ALL aged ≥60 years, or unfit for intensive chemotherapy and stem cell transplantation. Read More

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October 2021

Human iPSC-derived macrophages for efficient Staphylococcus aureus clearance in a murine pulmonary infection model.

Blood Adv 2021 Oct 14. Epub 2021 Oct 14.

Hannover Medical School, Hannover, Germany.

Primary or secondary immunodeficiencies are characterized by disruption of the cellular and humoral immunity. Respiratory infections are a major cause of morbidity and mortality among immunodeficient or immunocompromised patients with Staphylococcus aureus being a common offending organism. We here propose an adoptive macrophage transfer approach aiming to enhance impaired pulmonary immunity against S. Read More

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October 2021

Ataxia Telangiectasia and Rad3-Related Activation by DNA Damage Mitigates Maladaptive Repair after Acute Kidney Injury.

Nephron 2021 Oct 14:1-4. Epub 2021 Oct 14.

Division of Renal Medicine, Brigham and Women's Hospital, Boston, Massachusetts, USA.

DNA damage is an important consequence of injury to the proximal tubule. The proximal tubule cell responds to this damage by mounting a DNA damage response (DDR). Two protein kinases, ataxia-telangiectasia mutated (ATM) or ataxia telangiectasia and Rad3-related (ATR), play an important role in this DDR. Read More

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October 2021

Generation of induced pluripotent stem cell lines from peripheral blood mononuclear cells of three drug resistant and three drug responsive epilepsy patients.

Stem Cell Res 2021 Oct 9;56:102564. Epub 2021 Oct 9.

The University of Queensland, UQ Centre for Clinical Research, Herston, Brisbane, QLD 4029, Australia; Department of Neurology, Royal Brisbane and Women's Hospital, Herston, Brisbane, QLD 4029, Australia.

Epilepsy is a common neurological disorder characterized by seizures. Unfortunately, 30-40% of all epilepsy patients are resistant to at least two or more anti-seizure medications. Attempts to treat these patients and prevent further seizures necessitates multiple drug trials for the patient. Read More

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October 2021

Anti-GD2 CAR MSCs against metastatic Ewing's sarcoma.

Transl Oncol 2021 Oct 11;15(1):101240. Epub 2021 Oct 11.

Division of Oncology, Department of Medical and Surgical Sciences for Children & Adults, University-Hospital of Modena and Reggio Emilia, Modena, Italy; Rigenerand Srl, Medolla, Modena, Italy. Electronic address:

Background: Ewing's sarcoma (ES) is an aggressive cancer affecting children and young adults. We pre-clinically demonstrated that mesenchymal stromal/stem cells (MSCs) can deliver tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) against primary ES after local injection. However, ES is often metastatic calling for approaches able to support MSC targeting to the ES multiple remote sites. Read More

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October 2021

Exogenous silicon enhances the systemic defense of cucumber leaves and roots against CA-induced autotoxicity stress by regulating the ascorbate-glutathione cycle and photosystem II.

Ecotoxicol Environ Saf 2021 Oct 11;227:112879. Epub 2021 Oct 11.

College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China; Gansu Provincial Key Laboratory of Arid Land Crop Science, Gansu Agricultural University, Lanzhou 730070, China. Electronic address:

Cinnamic acid (CA), one of the main autotoxins secreted by cucumber roots during continuous cropping, inhibits plant growth and reduces yield. Silicon (Si) is an environmentally friendly element that alleviates abiotic stresses in plants, but the mechanism underlying its resistance to autotoxicity remain unclear. Here, we used 0. Read More

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October 2021

The Role of Long Term Label-Retaining Cells in the Treatment of Erectile Dysfunction by Vacuum Erectile Device.

Sex Med 2021 Oct 11;9(6):100442. Epub 2021 Oct 11.

Department of Surgery, The University of Texas Health Science Center at Houston, Houston, TX, USA; Department of Urology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. Electronic address:

Introduction: Vacuum erectile device (VED) therapy is commonly used for penile rehabilitation after radical prostatectomy, however, the underlying mechanism of this effect is not fully understood.

Aim: To evaluate the presence of label-retaining cells (LRCs), cells with long-term retention of 5-ethynyl-2-deoxyuridine (EdU) labeling and recognized as adult stem cells or progenitor-like cells, in cavernous tissue after VED treatment using a BCNC rat model.

Methods: Postnatal pups (1 day old) of Sprague Dawley (SD) rats were intraperitoneally injected with EdU (50 ug/g, BID for 3 days) and BCNC surgery was conducted at 6 weeks old (designated as natal-labeled rats). Read More

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October 2021

HEMATOPOIETIC CELL TRANSPLANTATION IS FEASIBLE IN PATIENTS WITH PRIOR COVID-19 INFECTION.

Transplant Cell Ther 2021 Oct 11. Epub 2021 Oct 11.

Adult Bone Marrow Transplantation Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY; Department of Medicine, Weill Cornell Medical College, New York, NY. Electronic address:

Background: There are limited data on outcomes of patients with prior Coronavirus disease 2019 (COVID-19) who proceeded to autologous or allogeneic hematopoietic cell transplantation (HCT). It is unknown whether these patients are more susceptible to poor outcomes and recurrence of COVID-19.

Objective: We report a retrospective analysis of outcomes of 15 consecutive patients with hematologic malignancies who experienced COVID-19 and subsequently underwent autologous (n=8) or allogeneic (n=7) HCT between 6/17/20 and 2/17/21. Read More

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October 2021

Upfront Alternative Donor Transplant Versus Immunosuppressive Therapy in Patients with Severe Aplastic Anemia Who Lack Fully HLA Matched Related Donor: Systematic Review and Meta-Analysis of Retrospective Studies. on Behalf of the Severe Aplastic Anemia Working Party of European Group for Blood and Marrow Transplantation (SAAWP of EBMT).

Transplant Cell Ther 2021 Oct 11. Epub 2021 Oct 11.

King Fahad Medical City, Riyadh, Saudi Arabia. Electronic address:

Idiopathic aplastic anemia is a rare and life-threatening disorder with hematopoietic stem cell transplant (HSCT) from matched sibling donor (MSD) being the standard treatment strategy for young patients. The use of alternative donor transplant (ADT) from a matched unrelated donor (MUD) or HLA haploidentical donor (HID) is not commonly used in the frontline setting. The aim of this systematic review/meta-analysis is to compare ADT as an upfront, rather than delayed, treatment strategy in the absence of a MSD to immunosuppressive therapy (IST) in severe aplastic anemia (SAA). Read More

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October 2021

Epigenome-Metabolome-Epigenome signaling cascade in cell biological processes.

J Genet Genomics 2021 Oct 11. Epub 2021 Oct 11.

CAS Key Laboratory of Regenerative Biology, Joint School of Life Sciences, Guangzhou Medical University; Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China; Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, CUHK-GIBH Joint Research Laboratory on Stem Cells and Regenerative Medicine, Institute for Stem Cell and Regeneration, Guangzhou Institutes of Biomedicine and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Guangzhou, 510530, China; Centre for Regenerative Medicine and Health, Hong Kong Institute of Science & Innovation, Chinese Academy of Sciences, Hong Kong SAR, China. Electronic address:

Cell fate determination as a fundamental question in cell biology has been extensively studied at different regulatory levels for many years. However, the mechanisms of multi-level regulation of cell fate determination remain unclear. Recently we have proposed an Epigenome-Metabolome-Epigenome (E-M-E) signaling cascade model to describe the crossover cooperation during mouse somatic cell reprogramming. Read More

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October 2021

TRIP4 transcriptionally activates DDIT4 and subsequent mTOR signaling to promote glioma progression.

Free Radic Biol Med 2021 Oct 11. Epub 2021 Oct 11.

Sun Yat-sen University Cancer Center; State Key Laboratory of Oncology in South China; Collaborative Innovation Center of Cancer Medicine, Guangzhou, China. Electronic address:

In spite of significant advances in the understanding of glioma biology and pathology, survival remains poor. Therefore, it is still of great significance to further explore the key factors involved in tumorigenesis and development in glioma and find potential new therapeutic targets. Here, we show that thyroid hormone receptor interactor 4 (TRIP4) is highly expressed in glioma cells and tissues. Read More

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October 2021

Simultaneous quantification of 26 NAD-related metabolites in plasma, blood, and liver tissue using UHPLC-MS/MS.

Anal Biochem 2021 Oct 12;633:114409. Epub 2021 Oct 12.

Developmental and Stem Cell Biology Division, Victor Chang Cardiac Research Institute, Sydney, New South Wales, 2010, Australia; Faculty of Medicine, University of New South Wales, Sydney, New South Wales, 2052, Australia; Faculty of Science, University of New South Wales, Sydney, New South Wales, 2052, Australia. Electronic address:

Nicotinamide adenine dinucleotide (NAD) is a key metabolic intermediate found in all cells and involved in numerous cellular functions. Perturbances in the NAD metabolome are linked to various diseases such as diabetes and schizophrenia, and to congenital malformations and recurrent miscarriage. Mouse models are central to the investigation of these and other NAD-related conditions because mice can be readily genetically modified and treated with diets with altered concentrations of NAD precursors. Read More

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October 2021

Smart and pH-sensitive rGO/Arabinoxylan/chitosan composite for wound dressing: In-vitro drug delivery, antibacterial activity, and biological activities.

Int J Biol Macromol 2021 Oct 11. Epub 2021 Oct 11.

Department of Biological Sciences, National University of Medical Sciences, Rawalpindi, Punjab, Pakistan. Electronic address:

Carbohydrate polymers are biological macromolecules that have sparked a lot of interest in wound healing due to their outstanding antibacterial properties and sustained drug release. Arabinoxylan (ARX), Chitosan (CS), and reduced graphene oxide (rGO) sheets were combined and crosslinked using tetraethyl orthosilicate (TEOS) as a crosslinker to fabricate composite hydrogels and assess their potential in wound dressing for skin wound healing. Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), and biological assays were used to evaluate the composite hydrogels. Read More

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October 2021

Anti-gastric cancer activity of cultivated Dendrobium huoshanense stem polysaccharide in tumor-bearing mice: Effects of molecular weight and O-acetyl group.

Int J Biol Macromol 2021 Oct 11;192:590-599. Epub 2021 Oct 11.

School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, People's Republic of China; Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230009, People's Republic of China. Electronic address:

The present study aimed at assuring whether homogeneous cultivated Dendrobium huoshanense stem polysaccharide (cDHPS) could inhibit gastric cancer in vivo, and whether its anti-gastric cancer activity could be affected by its molecular weight and O-acetyl group. Three different fractions (cDHPS-I, cDHPS-II and cDHPS-III) with decreased molecular weights and one fraction (cDHPS-IV) without O-acetyl group were prepared from cDHPS. Their structures were identified systematically. Read More

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October 2021

Counter Piece to Organoid vs Mouse Models: Which is a Better Research Tool….

Cell Mol Gastroenterol Hepatol 2021 Oct 11. Epub 2021 Oct 11.

Biotech Research and Innovation Center, Novo Nordisk Foundation Center for Stem Cell Biology (DanStem), Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. Electronic address:

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October 2021

Characterization of Differentially Expressed miRNAs by CXCL12/SDF-1 in Human Bone Marrow Stromal Cells.

Biomol Concepts 2021 Oct 13;12(1):132-143. Epub 2021 Oct 13.

Department of Orthopedics, Augusta University, Augusta, GA.

Stromal cell-derived factor 1 (SDF-1) is known to influence bone marrow stromal cell (BMSC) migration, osteogenic differentiation, and fracture healing. We hypothesize that SDF-1 mediates some of its effects on BMSCs through epigenetic regulation, specifically via microRNAs (miRNAs). MiRNAs are small non-coding RNAs that target specific mRNA and prevent their translation. Read More

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October 2021

Tumor DNA-Methylome derived Epigenetic Fingerprint Identifies HPV-negative Head and Neck Patients at Risk for Locoregional Recurrence after Postoperative Radiochemotherapy.

Int J Cancer 2021 Oct 14. Epub 2021 Oct 14.

Department of Otorhinolaryngology, Head and Neck Surgery, Heidelberg University Hospital, Heidelberg, Germany.

Biomarkers with relevance for loco-regional therapy are needed in Human Papillomavirus negative aka HPV(-) Head and Neck Squamous Cell Carcinoma (HNSCC). Based on the premise that DNA methylation pattern is highly conserved, we sought to develop a reliable and robust methylome-based classifier identifying HPV(-) HNSCC patients at risk for loco-regional recurrence (LR) and all-event progression after postoperative radiochemotherapy (PORT-C). The training cohort consisted of HPVDNA negative HNSCC patients (n=128) homogeneously treated with PORT-C in frame of the German Cancer Consortium - Radiation Oncology Group (DKTK-ROG) multicenter biomarker trial. Read More

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October 2021

U2 snRNA structure is influenced by SF3A and SF3B proteins but not by SF3B inhibitors.

PLoS One 2021 14;16(10):e0258551. Epub 2021 Oct 14.

Department of Molecular Cell and Developmental Biology, University of California, Santa Cruz, California, United States of America.

U2 snRNP is an essential component of the spliceosome. It is responsible for branch point recognition in the spliceosome A-complex via base-pairing of U2 snRNA with an intron to form the branch helix. Small molecule inhibitors target the SF3B component of the U2 snRNP and interfere with A-complex formation during spliceosome assembly. Read More

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October 2021

Patterning with clocks and genetic cascades: Segmentation and regionalization of vertebrate versus insect body plans.

PLoS Genet 2021 Oct 14;17(10):e1009812. Epub 2021 Oct 14.

Division of Developmental Biology, Department of Biology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.

Oscillatory and sequential processes have been implicated in the spatial patterning of many embryonic tissues. For example, molecular clocks delimit segmental boundaries in vertebrates and insects and mediate lateral root formation in plants, whereas sequential gene activities are involved in the specification of regional identities of insect neuroblasts, vertebrate neural tube, vertebrate limb, and insect and vertebrate body axes. These processes take place in various tissues and organisms, and, hence, raise the question of what common themes and strategies they share. Read More

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October 2021

The Effect of Surgical Approach and Femoral Prosthesis Type on Revision Rates Following Total Hip Arthroplasty: An Analysis of the Most Commonly Utilized Cementless Stems.

J Bone Joint Surg Am 2021 Oct 14. Epub 2021 Oct 14.

Traumaplasty Melbourne, East Melbourne, Victoria, Australia.

Background: Compared with other total hip arthroplasty (THA) approaches, the anterior approach has an increased rate of revision for femoral-sided complications, and certain stems may increase this risk. The present study aimed to assess the outcome of THA by surgical approach, according to the femoral stem utilized in the procedure.

Methods: Data from the Australian Orthopaedic Association National Joint Replacement Registry were analyzed for patients undergoing primary THA for osteoarthritis via the anterior or posterior approach with use of 1 of 5 of the most common cementless femoral stems from January 2015 to December 2019. Read More

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October 2021

Generation of Urine-Derived Induced Pluripotent Stem Cell Line from Patients with Acute Kidney Injury.

Cell Reprogram 2021 Oct;23(5):290-303

Department of Nephrology, Sir Run Run Hospital, Nanjing Medical University, Nanjing, China.

Acute kidney injury (AKI) is mainly characterized by rapid decline of renal function. Currently, the strategy of stem cells might be a therapy to treat AKI. The objective of this study was to obtain human urine-derived cells (HUCs) from patients with AKI, followed by establishing induced pluripotent stem (iPS) cell line. Read More

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October 2021

Effect of Embryo Aggregation on Development of Adipose-Derived Mesenchymal Stem Cell-Derived Bovine Clones.

Cell Reprogram 2021 Oct;23(5):277-289

Laboratorio Biotecnología Animal (LabBA), Dto Producción Animal, Facultad de Agronomía, Universidad de Buenos Aires, Buenos Aires, Argentina.

Somatic cell nuclear transfer (SCNT) is a method with unique ability to reprogram the epigenome of a fully differentiated cell. However, its efficiency remains extremely low. In this work, we assessed and combined two simple strategies to improve the SCNT efficiency in the bovine. Read More

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October 2021

Modern approaches on stem cells and scaffolding technology for osteogenic differentiation and regeneration.

Exp Biol Med (Maywood) 2021 Oct 14:15353702211052927. Epub 2021 Oct 14.

Department of Bioscience Research, University of Tennessee Health Science Center, Memphis, TN 38163, USA.

The process of bone repair has always been a natural mystery. Although bones do repair themselves, supplemental treatment is required for the initiation of the self-regeneration process. Predominantly, surgical procedures are employed for bone regeneration. Read More

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October 2021

Efficacy and toxicity of high-risk therapy of the Dutch Childhood Oncology Group in childhood acute lymphoblastic leukemia.

Pediatr Blood Cancer 2021 Oct 14:e29387. Epub 2021 Oct 14.

Princess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.

Background: Children with acute lymphoblastic leukemia (ALL) and high-risk (HR) features have a poor outcome and are treated with HR blocks, often followed by allogenic stem cell transplantation (SCT).

Procedure: This article analyses the outcomes of children treated with HR blocks between 2004 and 2017 according to DCOG ALL10/11 protocols. 1297 patients with newly diagnosed ALL were consecutively enrolled, of which 107 met the HR criteria (no complete remission; minimal residual disease (MRD) > 10 after consolidation; "MLL-AF4" translocation and in ALL-10 also poor prednisone response). Read More

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October 2021

Future Directions in Chronic Phase CML Treatment.

Curr Hematol Malig Rep 2021 Oct 14. Epub 2021 Oct 14.

Leukemia Center, Massachusetts General Hospital, 55 Fruit St, Boston, MA, 02114, USA.

Purpose Of Review: This review will focus on recent and emerging treatment paradigms in chronic phase CML. The discussion of each novel treatment or drug combination will include a brief overview of scientific rational and pre-clinical data, followed by recently published or ongoing clinical trial efforts. The review will be divided into three focus areas in CML treatment: new frontline approaches and approaches to deepen remission, second treatment-free remission studies, and the treatment of refractory disease. Read More

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October 2021

Cardiomyocyte Na and Ca mishandling drives vicious cycle involving CaMKII, ROS, and ryanodine receptors.

Basic Res Cardiol 2021 Oct 14;116(1):58. Epub 2021 Oct 14.

Department of Pharmacology, University of California, Davis, 451 Health Sciences Drive, Davis, CA, 95616, USA.

Cardiomyocyte Na and Ca mishandling, upregulated Ca/calmodulin-dependent kinase II (CaMKII), and increased reactive oxygen species (ROS) are characteristics of various heart diseases, including heart failure (HF), long QT (LQT) syndrome, and catecholaminergic polymorphic ventricular tachycardia (CPVT). These changes may form a vicious cycle of positive feedback to promote cardiac dysfunction and arrhythmias. In HF rabbit cardiomyocytes investigated in this study, the inhibition of CaMKII, late Na current (I), and leaky ryanodine receptors (RyRs) all attenuated the prolongation and increased short-term variability (STV) of action potential duration (APD), but in age-matched controls these inhibitors had no or minimal effects. Read More

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October 2021

Germline competent mesoderm: the substrate for vertebrate germline and somatic stem cells?

Biol Open 2021 Oct 14;10(10). Epub 2021 Oct 14.

School of Life Sciences, Division of Cells, Organisms and Molecular Genetics, University of Nottingham, Medical School, Queen's Medical Centre, Nottingham, NG7 2UH, UK.

In vitro production of tissue-specific stem cells [e.g. haematopoietic stem cells (HSCs)] is a key goal of regenerative medicine. Read More

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October 2021

Maternal iron deficiency alters trophoblast differentiation and placental development in rat pregnancy.

Endocrinology 2021 Oct 14. Epub 2021 Oct 14.

Department of Anatomy and Cell Biology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada.

Iron deficiency occurs when iron demands chronically exceed intake, and is prevalent in pregnant women. Iron deficiency during pregnancy poses major risks for the baby, including fetal growth restriction and long-term health complications. The placenta serves as the interface between a pregnant mother and her baby, and ensures adequate nutrient provisions for the fetus. Read More

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October 2021