8,741 results match your criteria starvation conditions

Metallo-β-lactamases in the Age of Multidrug Resistance: From Structure and Mechanism to Evolution, Dissemination, and Inhibitor Design.

Chem Rev 2021 Jun 15. Epub 2021 Jun 15.

Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, Universidad Nacional de Rosario, Ocampo y Esmeralda S/N, 2000 Rosario, Argentina.

Antimicrobial resistance is one of the major problems in current practical medicine. The spread of genes coding for resistance determinants among bacteria challenges the use of approved antibiotics, narrowing the options for treatment. Resistance to carbapenems, last resort antibiotics, is a major concern. Read More

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Transcriptome Analysis Reveals Cr(VI) Adaptation Mechanisms in sp. Strain AqSCr.

Front Microbiol 2021 27;12:656589. Epub 2021 May 27.

Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Mexico.

sp. strain AqSCr, isolated from Cr(VI)-polluted groundwater, reduces Cr(VI) both aerobically and anaerobically and resists up 34 mM Cr(VI); this resistance is independent of the ChrA efflux transporter. In this study, we report the whole genome sequence and the transcriptional profile by RNA-Seq of strain AqSCr under Cr(VI)-adapted conditions and found 255 upregulated and 240 downregulated genes compared to controls without Cr(VI) supplementation. Read More

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Starvation induces shrinkage of the bacterial cytoplasm.

Proc Natl Acad Sci U S A 2021 Jun;118(24)

Department of Biology, Washington University in St. Louis, St. Louis, MO 63130;

Environmental fluctuations are a common challenge for single-celled organisms; enteric bacteria such as experience dramatic changes in nutrient availability, pH, and temperature during their journey into and out of the host. While the effects of altered nutrient availability on gene expression and protein synthesis are well known, their impacts on cytoplasmic dynamics and cell morphology have been largely overlooked. Here, we discover that depletion of utilizable nutrients results in shrinkage of 's inner membrane from the cell wall. Read More

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Genome-wide analysis of long non-coding RNAs (lncRNAs) in two contrasting soybean genotypes subjected to phosphate starvation.

BMC Genomics 2021 Jun 9;22(1):433. Epub 2021 Jun 9.

Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Zhengzhou, 450002, China.

Background: Phosphorus (P) is essential for plant growth and development, and low-phosphorus (LP) stress is a major factor limiting the growth and yield of soybean. Long noncoding RNAs (lncRNAs) have recently been reported to be key regulators in the responses of plants to stress conditions, but the mechanism through which LP stress mediates the biogenesis of lncRNAs in soybean remains unclear.

Results: In this study, to explore the response mechanisms of lncRNAs to LP stress, we used the roots of two representative soybean genotypes that present opposite responses to P deficiency, namely, a P-sensitive genotype (Bogao) and a P-tolerant genotype (NN94156), for the construction of RNA sequencing (RNA-seq) libraries. Read More

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Zeolitic Imidazolate Frameworks (ZIF-8) for Biomedical Applications: A Review.

Curr Med Chem 2021 Jun 8. Epub 2021 Jun 8.

Advanced Multifunctional Materials Laboratory, Department of Chemistry, Assiut University, Assiut, 71516, Egypt.

Beyond being an excellent protective material for bioentities, zeolitic imidazolate frameworks (ZIF-8) have advanced several applications, including biomedical applications. The straightforward synthesis of ZIF-8 at mild conditions improved the biomineralization of several biomolecules, e.g. Read More

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Long-distance blue light signalling regulates phosphate deficiency-induced primary root growth inhibition.

Mol Plant 2021 Jun 5. Epub 2021 Jun 5.

National Key Laboratory of Plant Molecular Genetics (NKLPMG), CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China. Electronic address:

Although roots are mainly embedded in the soil, recent studies revealed that light regulates mineral nutrient uptake by roots. However, it remains unclear whether the root system architecture (RSA) change in response to different rhizosphere nutrient statuses involves light signalling. Here we report that blue light regulates primary root growth inhibition under phosphate-deficient conditions through the cryptochromes and their downstream signalling factors. Read More

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Msn2/4 transcription factors positively regulate expression of Atg39 ER-phagy receptor.

Sci Rep 2021 Jun 7;11(1):11919. Epub 2021 Jun 7.

Department of Molecular Cell Biology, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, 305-8575, Japan.

Selective autophagy requires the autophagy receptor specifically localizing to the target for degradation. In the budding yeast, Atg39 and Atg40 function as an autophagy receptor for the endoplasmic reticulum (ER)-selective autophagy, referred to as ER-phagy. The expression level of the ATG39 gene is increased in response to ER stress and nitrogen starvation. Read More

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Low nitrogen availability inhibits the phosphorus starvation response in maize (Zea mays ssp. mays L.).

BMC Plant Biol 2021 Jun 5;21(1):259. Epub 2021 Jun 5.

Laboratorio Nacional de Genómica para la Biodiversidad (Langebio), Unidad de Genómica Avanzada, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Irapuato, C.P, 36824, Guanajuato, Mexico.

Background: Nitrogen (N) and phosphorus (P) are macronutrients essential for crop growth and productivity. In cultivated fields, N and P levels are rarely sufficient, contributing to the gap between realized and potential production. Fertilizer application increases nutrient availability, but is not available to all farmers, nor are current rates of application sustainable or environmentally desirable. Read More

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Regulation of autophagy flux by E3 ubiquitin ligase Pirh2 in lung cancer.

Biochem Biophys Res Commun 2021 Jul 2;563:119-125. Epub 2021 Jun 2.

Institute of Cytology, Russian Academy of Sciences, 194064, St Petersburg, Russian Federation; Moscow Institute of Physics and Technology, Dolgoprudny, 141700, Moscow region, Russian Federation. Electronic address:

Autophagy is a special catabolic cellular program that is induced in response to deprivation of nutrients and energy starvation. During the execution of this program, cellular components, including aggregates, as well as damaged organelles and some proteins are encapsulated in special vesicles known as autophagosomes and subsequently are degraded after fusion of autophagosomes with lysosomes. Importantly, at late stages of tumorigenesis cancer cells employ autophagy to sustain proliferation in unfavorable conditions, including anti-cancer drug therapy. Read More

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Integration of Dual Stress Transcriptomes and Major QTLs from a Pair of Genotypes Contrasting for Drought and Chronic Nitrogen Starvation Identifies Key Stress Responsive Genes in Rice.

Rice (N Y) 2021 Jun 5;14(1):49. Epub 2021 Jun 5.

ICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi, 110012, India.

We report here the genome-wide changes resulting from low N (N-W+), low water (N+W-)) and dual stresses (N-W-) in root and shoot tissues of two rice genotypes, namely, IR 64 (IR64) and Nagina 22 (N22), and their association with the QTLs for nitrogen use efficiency. For all the root parameters, except for root length under N-W+, N22 performed better than IR64. Chlorophyll a, b and carotenoid content were higher in IR64 under N+W+ treatment and N-W+ and N+W- stresses; however, under dual stress, N22 had higher chlorophyll b content. Read More

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Short and long sleeping mutants reveal links between sleep and macroautophagy.

Elife 2021 Jun 4;10. Epub 2021 Jun 4.

Chronobiology and Sleep Institute, Perelman Medical School of University of Pennsylvania, Philadelphia, United States.

Sleep is a conserved and essential behavior, but its mechanistic and functional underpinnings remain poorly defined. Through unbiased genetic screening in , we discovered a novel short-sleep mutant we named . Positional cloning and subsequent complementation, CRISPR/Cas9 knock-out, and RNAi studies identified Argus as a transmembrane protein that acts in adult peptidergic neurons to regulate sleep. Read More

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Biomineralized synthesis of a smart O-regenerating nanoreactor for highly efficient starvation/gas therapy.

Mater Sci Eng C Mater Biol Appl 2021 Jul 28;126:112132. Epub 2021 Apr 28.

The Key Laboratory of Life-Organic Analysis, School of Chemistry and Chemical Engineering, Qufu Normal University, Qufu 273165, PR China.

The emerging starvation therapy holds great promise in cancer treatment, however, its therapeutic effect is heavily reduced by intracellular hypoxia and high glutathione (GSH) conditions. To overcome these limitations, a new concept of starvation therapy pattern that employs biodegradable carriers with special selectivity and exhibits excellent anti-migration and therapy effect without using any invasive chemotherapy drugs was developed. A facile biomineralization method is first chosen to synthesize human serum albumin and folic acid modified MnO to guarantee active targeting, long-term stability and responsive degradation in tumor microenvironment. Read More

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Daphnia magna Gut Specific Trancriptomic Responses to Feeding Inhibiting Chemicals and Food Limitation.

Environ Toxicol Chem 2021 Jun 3. Epub 2021 Jun 3.

Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research, CSIC, Jordi Girobna 18, 08034, Barcelona, Spain.

Transcriptomic responses combined with apical adverse ecologically relevant outcomes have proven to be useful to unravel and anchor molecular mechanisms of action to adverse outcomes. This is the case for feeding inhibition responses in the model ecotoxicological species Daphnia magna. The aim of this study was to assess the transcriptomic responses in guts dissected from D. Read More

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FKBP39 controls nutrient dependent Nprl3 expression and TORC1 activity in Drosophila.

Cell Death Dis 2021 Jun 2;12(6):571. Epub 2021 Jun 2.

Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, 225009, China.

Target of Rapamycin Complex 1 (TORC1) is a master regulator that coordinates nutrient status with cell metabolism. The GTPase-activating protein towards Rags complex 1 (GATOR1) inhibits TORC1 activity and protects cells from damage during periods of stress. Here we characterize multiple pathways that regulate the expression of the GATOR1 component Nprl3 in Drosophila. Read More

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Significance of Adipose Tissue Maintenance in Patients Undergoing Hemodialysis.

Senji Okuno

Nutrients 2021 May 31;13(6). Epub 2021 May 31.

Kidney Center, Shirasagi Hospital, 7-11-23, Kumata, Higashisumiyoshi-ku, Osaka 546-0002, Japan.

In the general population, obesity is known to be associated with adverse outcomes, including mortality. In contrast, high body mass index (BMI) may provide a survival advantage for hemodialysis patients, which is known as the obesity paradox. Although BMI is the most commonly used measure for the assessment of obesity, it does not distinguish between fat and lean mass. Read More

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Self-Powered Smart Beehive Monitoring and Control System (SBMaCS).

Sensors (Basel) 2021 May 19;21(10). Epub 2021 May 19.

National Council of Science and Technology (NCST), Kigali 2285, Rwanda.

Beekeeping in Africa has been practiced for many years through successive generations and along inherited patterns. Beekeepers continue to face challenges in accessing consistent and business-driven markets for their bee products. In addition, the honeybee populations are decreasing due to colony collapse disorder (CCD), fire, loss of bees in swarming, honey buggers and other animals, moths, starvation, cold weather, and Varoa mites. Read More

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Overexpression of Transcripts Coding for Renin-b but Not for Renin-a Reduce Oxidative Stress and Increase Cardiomyoblast Survival under Starvation Conditions.

Cells 2021 May 14;10(5). Epub 2021 May 14.

Institute of Physiology, University Medicine Greifswald, 17475 Greifswald, Germany.

A stimulated renin-angiotensin system is known to promote oxidative stress, apoptosis, necrosis and fibrosis. Renin transcripts (renin-b; renin-c) encoding a cytosolic renin isoform have been discovered that may in contrast to the commonly known secretory renin (renin-a) exert protective effects Here, we analyzed the effect of renin-a and renin-b overexpression in H9c2 cardiomyoblasts on apoptosis and necrosis as well as on potential mechanisms involved in cell death processes. To mimic ischemic conditions, cells were exposed to glucose starvation, anoxia or combined oxygen-glucose deprivation (OGD) for 24 h. Read More

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Nujiangexanthone A Inhibits Cervical Cancer Cell Proliferation by Promoting Mitophagy.

Molecules 2021 May 12;26(10). Epub 2021 May 12.

Institute of Cardiovascular Disease of Integrated Traditional Chinese and Western Medicine, Shuguang Hospital affiliated to Shanghai University of Traditional Chinese Medicine, No. 528, Zhangheng Road, Shanghai 201203, China.

Nujiangexanthone A (NJXA), a bioactive component isolated from the leaves of , has been reported to exhibit anti-inflammatory, antioxidant, and antitumor effects. Our previous work has shown that NJXA induced G arrest and apoptosis, thus suppressing cervical cancer cell growth. The present study provides new evidence that NJXA can induce cell death in HeLa cells by promoting mitophagy. Read More

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Plastid Anionic Lipids Are Essential for the Development of Both Photosynthetic and Non-Photosynthetic Organs in .

Int J Mol Sci 2021 May 4;22(9). Epub 2021 May 4.

Department of Biological Science, Graduate School of Science, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, Sakai 599-8531, Japan.

The lipid bilayer matrix of the thylakoid membrane of cyanobacteria and chloroplasts of plants and algae is mainly composed of uncharged galactolipids, but also contains anionic lipids sulfoquinovosyldiacylglycerol (SQDG) and phosphatidylglycerol (PG) as major constituents. The necessity of PG for photosynthesis is evident in all photosynthetic organisms examined to date, whereas the requirement of SQDG varies with species. In plants, although PG and SQDG are also found in non-photosynthetic plastids, their importance for the growth and functions of non-photosynthetic organs remains unclear. Read More

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Genome Scale Analysis Reveals IscR Directly and Indirectly Regulates Virulence Factor Genes in Pathogenic .

mBio 2021 Jun 1:e0063321. Epub 2021 Jun 1.

Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, Santa Cruz, California, USA.

The iron-sulfur cluster coordinating transcription factor IscR is important for the virulence of Yersinia pseudotuberculosis and a number of other bacterial pathogens. However, the IscR regulon has not yet been defined in any organism. To determine the IscR regulon and identify IscR-dependent functions important for virulence, we employed chromatin immunoprecipitation sequencing (ChIP-Seq) and RNA sequencing (RNA-Seq) of Y. Read More

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Energy Starvation Induces a Cell Cycle Arrest in by Triggering Degradation of the DnaA Initiator Protein.

Front Mol Biosci 2021 13;8:629953. Epub 2021 May 13.

Department of Biology, University of Copenhagen, Copenhagen, Denmark.

During steady-state growth, the amount and activity of the initiator protein, DnaA, controls chromosome replication tightly so that initiation only takes place once per origin in each cell cycle, regardless of growth conditions. However, little is known about the mechanisms involved during transitions from one environmental condition to another or during starvation stress. ATP depletion is one of the consequences of long-term carbon starvation. Read More

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Serine Metabolism Regulates YAP Activity Through USP7 in Colon Cancer.

Front Cell Dev Biol 2021 12;9:639111. Epub 2021 May 12.

Central Laboratory, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, Jinhua, China.

Metabolic reprogramming is a vital factor in the development of many types of cancer, including colon cancer. Serine metabolic reprogramming is a major feature of tumor metabolism. Yes-associated protein (YAP) participates in organ size control and tumorigenesis. Read More

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Characterization of the DdrD protein from the extremely radioresistant bacterium Deinococcus radiodurans.

Extremophiles 2021 May 29. Epub 2021 May 29.

Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91198, Gif sur Yvette, France.

Here, we report the in vitro and in vivo characterization of the DdrD protein from the extraordinary stress-resistant bacterium, D. radiodurans. DdrD is one of the most highly induced proteins following cellular irradiation or desiccation. Read More

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Expression of potential reference genes in response to macronutrient stress in rice and soybean.

Gene 2021 Aug 27;792:145742. Epub 2021 May 27.

Mineral Nutrition Laboratory, Division of Plant Physiology, ICAR-Indian Agriculture Research Institute, New Delhi 110012, India. Electronic address:

Given the complexity of nutrient stress responses and the availability of a few validated reference genes, we aimed to identify robust and stable reference genes for macronutrient stress in rice and soybean. Ten potential reference genes were evaluated using geNorm, NormFinder, BestKeeper, Comparative ΔCt method, and RefFinder algorithms under low and completely starved conditions of nitrogen (N), phosphorus (P), potassium (K), and sulphur (S). Results revealed distinct sets of reference gene pairs, showing stable expression under different experimental conditions. Read More

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Interaction Between Macro- and Micro-Nutrients in Plants.

Front Plant Sci 2021 10;12:665583. Epub 2021 May 10.

Indian Council of Agricultural Research, New Delhi, India.

Nitrogen (N), phosphorus (P), sulfur (S), zinc (Zn), and iron (Fe) are some of the vital nutrients required for optimum growth, development, and productivity of plants. The deficiency of any of these nutrients may lead to defects in plant growth and decreased productivity. Plant responses to the deficiency of N, P, S, Fe, or Zn have been studied mainly as a separate event, and only a few reports discuss the molecular basis of biological interaction among the nutrients. Read More

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Convergent adaptation in mitochondria of phylogenetically distant birds: does it exist?

Genome Biol Evol 2021 May 25. Epub 2021 May 25.

Skolkovo Institute of Science and Technology, Ulitsa Nobelya, 3, Moscow, Moscow Oblast, Russia.

In a wide range of taxa, proteins encoded by mitochondrial genomes are involved in adaptation to lifestyle that requires oxygen starvation or elevation of metabolism rate. It remains poorly understood to what extent adaptation to similar conditions is associated with parallel changes in these proteins. We search for genetic signal of parallel or convergent evolution in recurrent molecular adaptation to high altitude, migration, diving, wintering, unusual flight abilities, or loss of flight in mitochondrial genomes of birds. Read More

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Refractive Index Sensing for Measuring Single Cell Growth.

ACS Nano 2021 May 24. Epub 2021 May 24.

Terasaki Institute for Biomedical Innovation, Los Angeles, California 90024, United States.

Accessing cell growth on adhesive substrates is critical for identifying biophysical properties of cells and their therapeutic response to drug therapies. However, optical techniques have low sensitivity, and their reliability varies with cell type, whereas microfluidic technologies rely on cell suspension. In this paper, we introduced a plasmonic functional assay platform that can precisely measure cell weight and the dynamic change in real-time for adherent cells. Read More

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An AMP-activated protein kinase complex with two distinctive alpha subunits is involved in nutritional stress responses in Trypanosoma cruzi.

PLoS Negl Trop Dis 2021 May 24;15(5):e0009435. Epub 2021 May 24.

Laboratorio de señalización y mecanismos adaptativos en tripanosomátidos, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres", Buenos Aires, Argentina.

Trypanosoma cruzi, the etiological agent of Chagas disease, has a digenetic life cycle. In its passage from the insect vector to the mammalian host, and vice versa, it must be prepared to cope with abrupt changes in environmental conditions, such as carbon source, pH, temperature and osmolarity, in order to survive. Sensing and signaling pathways that allow the parasite to adapt, have unique characteristics with respect to their hosts and other free-living organisms. Read More

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Proteomic analysis reveals the mechanism of different environmental stress-induced tolerance of Pseudomonas aeruginosa to monochloramine disinfection.

J Hazard Mater 2021 May 14;417:126082. Epub 2021 May 14.

Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730000, PR China. Electronic address:

Although drinking water disinfection proved to be an effective strategy to eliminate many pathogens, bacteria can still show disinfection tolerance in drinking water distribution systems. To date, the molecular mechanisms on how environmental stress affects the tolerance of Pseudomonas aeruginosa to monochloramine are not well understood. Here, we investigated how three stress conditions, namely starvation, low temperature, and starvation combined with low temperature, affected the monochloramine tolerance of Pseudomonas aeruginosa, an opportunistic pathogen commonly found in drinking water distribution systems. Read More

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New insights into the function of the proteins IsiC and IsiD from Synechocystis sp. PCC 6803 under iron limitation.

Appl Microbiol Biotechnol 2021 Jun 21;105(11):4693-4707. Epub 2021 May 21.

State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.

Iron is a common cofactor in biological processes such as respiration, photosynthesis, and nitrogen fixation. The genes isiC and isiD encode unknown proteins, and the growth of ΔisiC and ΔisiD mutants is inhibited under iron-deficient conditions. To study the regulatory mechanisms of IsiC and IsiD during iron starvation, we carried out transcriptome and metabolome sequencing. Read More

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