7,964 results match your criteria standard qpcr

Canine visceral leishmaniasis and Rhipicephalus sanguineus: evaluation and comparison of classical techniques.

Vet Res Commun 2021 Sep 23. Epub 2021 Sep 23.

School of Veterinary Medicine, São Paulo State University (Unesp), Araçatuba, São Paulo, Brazil.

The aim of this study was evaluating the association and correlation between the diagnostics tests used for Leishmania spp. detection in dogs and ticks. We evaluated 99 dogs and 990 Rhipicephalus sanguineus. Read More

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September 2021

Impact of Preanalytical and Analytical Methods on Cell-Free DNA Diagnostics.

Front Cell Dev Biol 2021 6;9:686149. Epub 2021 Sep 6.

Department of Medical Biology, School of Medicine, University of Zagreb, Zagreb, Croatia.

While tissue biopsy has for the longest time been the gold-standard in biomedicine, precision/personalized medicine is making the shift toward liquid biopsies. Cell-free DNA (cfDNA) based genetic and epigenetic biomarkers reflect the molecular status of its tissue-of-origin allowing for early and non-invasive diagnostics of different pathologies. However, selection of preanalytical procedures (including cfDNA isolation) as well as analytical methods are known to impact the downstream results. Read More

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September 2021

Downregulation of GNA15 Inhibits Cell Proliferation P38 MAPK Pathway and Correlates with Prognosis of Adult Acute Myeloid Leukemia With Normal Karyotype.

Front Oncol 2021 6;11:724435. Epub 2021 Sep 6.

Department of Hematology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Background: The prognosis of acute myeloid leukemia (AML) with a normal karyotype is highly heterogonous, and the current risk stratification is still insufficient to differentiate patients from high-risk to standard-risk. Changes in some genetic profiles may contribute to the poor prognosis of AML. Although the prognostic value of G protein subunit alpha 15 () in AML has been reported based on the GEO (Gene Expression Omnibus) database, the prognostic significance of has not been verified in clinical samples. Read More

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September 2021

Silica-Coated Iron Oxide Nanoparticles for DNA Isolation for Molecular Genetic Studies in Hematology.

Genet Test Mol Biomarkers 2021 Sep;25(9):611-614

Federal Research Center Krasnoyarsk Scientific Center, Siberian Branch of RAS, Krasnoyarsk, Russia.

To develop magnetic nanoparticles (MNPs) based on iron oxide for DNA isolation from blood cells for quantitative molecular genetic analyses of the V617F mutation in the Januskinase 2 () gene. MNPs were synthesized by the coprecipitation method and coated with tetraethyl orthosilicate (TEOS). The size and shape of the complexes were estimated using transmission electron microscopy. Read More

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September 2021

Increased Detection of Mycobacterium tuberculosis Disease Using a Tissue-Based Laboratory-Developed Polymerase Chain Reaction Assay Compared to Standard Diagnostics.

Am J Trop Med Hyg 2021 Sep 20. Epub 2021 Sep 20.

Section of Infectious Diseases, Yale University School of Medicine, New Haven, Connecticut.

Standard diagnostics for Mycobacterium tuberculosis (MTB) including acid-fast bacilli (AFB) smear and culture, and Xpert™ MTB/RIF real-time Polymerase Chain Reaction (RT-PCR; Xpert) have variable sensitivity and/or long turnaround times. We describe the clinical performance of a laboratory-developed tissue-based MTB PCR compared with AFB culture and Xpert using a composite reference standard (CRS). Over an 8-year period, MTB PCR was performed on pulmonary, pleural, or lymph node specimens for 36 patients. Read More

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September 2021

CRISPR detectives against SARS-CoV-2: a major setback against COVID-19 blowout.

Appl Microbiol Biotechnol 2021 Sep 20. Epub 2021 Sep 20.

National Institute of Pharmaceutical Education and Research, Kolkata, India.

The emergence of SARS-CoV-2 has brought the world to a standstill, and till date, effective treatments and diagnostics against this idiosyncratic pathogen are lacking. As compared to the standard WHO/CDC qPCR detection method, which consumes several hours for detection, CRISPR-based SHERLOCK, DETECTR, and FELUDA have emerged as rapid diagnostic tools for the detection of the RNA genome of SARS-CoV-2 within an hour with 100% accuracy, specificity, and sensitivity. These attributes of CRISPR-based detection technologies have taken themselves one step ahead of available detection systems and are emerging as an inevitable tool for quick detection of the virus. Read More

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September 2021

Multicenter study evaluating novel multi-specimen pooling assay for the detection of SARS-CoV-2: High sensitivity and high throughput testing.

J Microbiol Immunol Infect 2021 Sep 2. Epub 2021 Sep 2.

Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC. Electronic address:

Background/purpose: Mass screening for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is important to prevent the spread of coronavirus disease 2019 (COVID-19). Pooling samples can increase the number of tests processed. LabTurbo AIO 48 is an automated platform that allows ribonucleic acid extraction and sample analysis on the same instrument. Read More

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September 2021

Myrianthus arboreus P. Beauv improves insulin sensitivity in high fat diet-induced obese mice by reducing inflammatory pathways activation.

J Ethnopharmacol 2021 Sep 16:114651. Epub 2021 Sep 16.

CIBER-EHD, Department of Pharmacology, Center for Biomedical Research (CIBM), University of Granada, 18071, Granada, Spain; Instituto de Investigación Biosanitaria de Granada (ibs.GRANADA), 18012, Granada, Spain.

Ethnopharmacological Relevance: Metabolic syndrome is currently recognized as the major cause of morbidity, with dramatic complications on life expectancy and health status. Myrianthus arboreus is a medicinal plant traditionally used in local communities as a safe remedy in treating diabetes and other metabolic diseases.

Aim Of The Study: This study aimed to investigate the impact of a methanol extract of Myrianthus arboreus leaf (MAL) in a mice model of metabolic syndrome induced by a high-fat diet (HFD) intake. Read More

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September 2021

A comprehensive nanopore sequencing methodology deciphers the complete transcriptional landscape of cyclin dependent kinase 4 (CDK4) in human malignancies.

FEBS J 2021 Sep 17. Epub 2021 Sep 17.

Department of Biochemistry and Molecular Biology, National and Kapodistrian University of Athens, Athens, Greece.

CDK4 is a member of the cyclin-dependent kinases, a family of protein kinases with outstanding roles in signalling pathways, transcription regulation and cell division. Defective or overactivated CDK4/cyclin D1 pathway leads to enhanced cellular proliferation, thus being implicated in human cancers. Although the biological role of CDK4 has been extensively studied, its pre-mRNA processing mechanism under normal or pathological conditions is neglected. Read More

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September 2021

Isothermal amplification and fluorescent detection of SARS-CoV-2 and SARS-CoV-2 variant virus in nasopharyngeal swabs.

PLoS One 2021 17;16(9):e0257563. Epub 2021 Sep 17.

Department of Infectious Disease, College of Veterinary Medicine, University of Georgia, Athens, Georgia, United States of America.

The COVID-19 pandemic caused by the SARS-CoV-2 is a serious health threat causing worldwide morbidity and mortality. Real-time reverse transcription PCR (RT-qPCR) is currently the standard for SARS-CoV-2 detection. Although various nucleic acid-based assays have been developed to aid the detection of SARS-CoV-2 from COVID-19 patient samples, the objective of this study was to develop a diagnostic test that can be completed in 30 minutes without having to isolate RNA from the samples. Read More

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September 2021

Comparative study of different SARS-CoV-2 diagnostic techniques.

J Virol Methods 2021 Sep 13;298:114281. Epub 2021 Sep 13.

Department of Clinical Microbiology, IML and IdISSC, Hospital Clínico San Carlos, Madrid, Spain; Department of Medicine, Facultad de Medicina, Universidad Complutense, Madrid, Spain. Electronic address:

The rapid spread of SARS-CoV-2 led to the necessity of developing diagnostic tests for rapid virus detection. Many commercial platforms have appeared and have been approved for this purpose. In this study, 95 positive and 5 negative retrospective samples were analyzed by 4 different commercial RT-qPCR kits (TaqMan 2019nCoV Assay, Allplex™SARS-COV-2 Assay, FTD SARS-COV-2 Assay and qCOVID-19). Read More

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September 2021

The Challenge of Using an Antigen Test as a Screening Tool for SARS-CoV-2 Infection in an Emergency Department: Experience of a Tertiary Care Hospital in Southern Italy.

Biomed Res Int 2021 11;2021:3893733. Epub 2021 Sep 11.

Department of Biomedical Sciences and Human Oncology-Hygiene Section, University of Bari, P.zza G. Cesare 11, 70124 Bari, Italy.

Background: In emergency hospital settings, rapid diagnosis and isolation of SARS-CoV-2 patients are required. The aim of the study was to evaluate the performance of an antigen chemiluminescence enzymatic immunoassay (CLEIA) and compare it with that of Real-time Reverse transcription-Polymerase Chain Reaction (RT-qPCR), the gold standard assay, to assess its suitability as a rapid diagnostic method for managing patients in the emergency department (ED).

Methods: Consecutive patients with no previous history of SARS-CoV-2 infection attending the ED of the Policlinico Hospital of Bari between 23rd October and 4th November 2020 were enrolled. Read More

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September 2021

Detection of soybean transgenic event GTS-40-3-2 using electric field-induced release and measurement (EFIRM).

Anal Bioanal Chem 2021 Sep 14. Epub 2021 Sep 14.

Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, College of Life Science, Dalian Minzu University, Dalian, 116600, China.

Polymerase chain reaction (PCR) technology has become a standard technique for the detection of genetically modified organisms (GMOs). However, this method requires a PCR amplification process which is both expensive and time-consuming. Herein, we propose electric field-induced release and measurement (EFIRM) technology as an alternative method for GMO screening. Read More

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September 2021

Effects of adding L-arginine orally to standard therapy in patients with COVID-19: A randomized, double-blind, placebo-controlled, parallel-group trial. Results of the first interim analysis.

EClinicalMedicine 2021 Sep 9:101125. Epub 2021 Sep 9.

Department of Advanced Biomedical Sciences, "Federico II" University, Naples, Italy.

Background: We and others have previously demonstrated that the endothelium is a primary target of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and L-arginine has been shown to improve endothelial dysfunction. However, the effects of L-arginine have never been evaluated in coronavirus disease 2019 (COVID-19).

Methods: This is a parallel-group, double-blind, randomized, placebo-controlled trial conducted on patients hospitalized for severe COVID-19. Read More

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September 2021

Concordance between PCR-based extraction-free saliva and nasopharyngeal swabs for SARS-CoV-2 testing.

HRB Open Res 2021 30;4:85. Epub 2021 Jul 30.

School of Pharmacy and Biomolecular Sciences, Royal College of Surgeons in Ireland, Dublin, Ireland.

: Saliva represents a less invasive alternative to nasopharyngeal swab (NPS) for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection. SalivaDirect is a nucleic acid extraction-free method for detecting SARS-CoV2 in saliva specimens. Studies evaluating the concordance of gold standard NPS and newly developed SalivaDirect protocols are limited. Read More

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Multiplex Real-Time Polymerase Chain Reaction on Sputum for the Diagnosis of Pneumonia in Children: A Retrospective Study.

Infect Drug Resist 2021 7;14:3627-3635. Epub 2021 Sep 7.

Department of Pediatrics, Xiangya Hospital, Central South University, Changsha, People's Republic of China.

Background: pneumonia (PCP) is a serious opportunistic infection in immunocompromised children. Real-time polymerase chain reaction (PCR) is widely used for the diagnosis of PCP due to its good accuracy. However, the diagnostic performance of multiplex real-time PCR on sputum in children with PCP has never been explored. Read More

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September 2021

Longitudinal Dynamics of a Blood Transcriptomic Signature of Tuberculosis.

Am J Respir Crit Care Med 2021 Sep 14. Epub 2021 Sep 14.

CAPRISA, Durban, South Africa.

Objectives We evaluated longitudinal kinetics of an 11-gene blood transcriptomic tuberculosis (TB) signature, RISK11, and effects of TB preventative therapy (TPT) and respiratory organisms on RISK11 signature score, in HIV-uninfected and HIV-infected individuals. Methods RISK11 was measured in a longitudinal study of RISK11-guided TPT in HIV-uninfected adults, a cross-sectional respiratory organisms cohort or a longitudinal study in people living with HIV (PLHIV). HIV-uninfected RISK11+ participants were randomised to TPT or no TPT; RISK11- participants received no TPT. Read More

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September 2021

Nested pool testing strategy for the diagnosis of infectious diseases.

Sci Rep 2021 09 13;11(1):18108. Epub 2021 Sep 13.

Departamento de Física & IFIBA, FCEN-UBA & CONICET, C1428EGA, Buenos Aires, Argentina.

The progress of the SARS-CoV-2 pandemic requires the design of large-scale, cost-effective testing programs. Pooling samples provides a solution if the tests are sensitive enough. In this regard, the use of the gold standard, RT-qPCR, raises some concerns. Read More

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September 2021

Improved diagnosis of gastrointestinal infections using a semi-automated multiplex real-time PCR for detection of enteropathogens.

J Med Microbiol 2021 Sep;70(9)

Department of Microbiology, Hospital Clinic, Barcelona, Spain.

The identification of enteropathogens is critical for the clinical management of patients with suspected gastrointestinal infection. The FLOW multiplex PCR system (FMPS) is a semi-automated platform (FLOW System, Roche) for multiplex real-time PCR analysis. FMPS has greater sensitivity for the detection of enteric pathogens than standard methods such as culture, biochemical identification, immunochromatography or microscopic examination. Read More

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September 2021

Comparative Evaluation of Nasopharyngeal and Oropharyngeal Swab Based Rapid SARS-CoV-2 Antigen Detection and Real-Time RT-PCR for Diagnosis of COVID-19 in Tertiary Care Hospital.

Cureus 2021 Jul 31;13(7):e16785. Epub 2021 Jul 31.

Microbiology, All India Institute of Medical Sciences, Rishikesh, Rishikesh, IND.

Purpose: To assess and compare the diagnostic accuracy of the GenBody COVID-19 Antigen kit (GenBody Inc., Cheonan, South Korea) available in the market with the gold standard reverse transcription-polymerase chain reaction (RT-PCR) assay to detect severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).

Methods: Nasopharyngeal and oropharyngeal swabs were collected from suspected coronavirus disease 2019 (COVID-19) patients and tested by RT-PCR and GenBody Rapid antigen kit. Read More

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Fructose-Rich Diet Attenuates Stress-Induced Metabolic Disturbances in the Liver of Adult Female Rats.

J Nutr 2021 Sep 11. Epub 2021 Sep 11.

Department of Biochemistry, Institute for Biological Research "Siniša Stanković", National Institute of Republic of Serbia, University of Belgrade, Belgrade, Serbia.

Background: Both fructose consumption and chronic stress contribute to the development of metabolic disorders. The consequences of such combination are not fully understood.

Objective: We investigated whether fructose supplementation and chronic stress synergistically disturb hepatic lipid and glucose metabolism. Read More

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September 2021

COVID-19 Diagnosed by Real-Time Reverse Transcriptase-Polymerase Chain Reaction in Nasopharyngeal Specimens of Suspected Cases in a Tertiary Care Center: A Descriptive Cross-sectional Study.

JNMA J Nepal Med Assoc 2021 May 25;59(237):464-467. Epub 2021 May 25.

Program & Department of Clinical Laboratory Medicine, Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, China.

Introduction: The emergence of severe acute respiratory syndrome coronavirus-2 pandemic is critically challenging the whole world. The real-time reverse transcriptase-polymerase chain reaction is the most widely used confirmatory test for COVID-19 detection. This study aimed to find out the prevalence of COVID-19 infection detected by gold standard reverse transcriptase-polymerase chain reaction test in a tertiary care center of Nepal. Read More

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Identification of reference genes for gene expression studies among different developmental stages of murine hearts.

BMC Dev Biol 2021 Sep 8;21(1):13. Epub 2021 Sep 8.

State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, 167A Beilishi Road, Xi Cheng District, Beijing, 100037, People's Republic of China.

Background: Real-time quantitative polymerase chain reaction (RT-qPCR) is a widely-used standard assay for assessing gene expression. RT-qPCR data requires reference genes for normalization to make the results comparable. Therefore, the selected reference gene should be highly stable in its expression throughout the experimental datasets. Read More

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September 2021

Molecular impact of launch related dynamic vibrations and static hypergravity in planarians.

NPJ Microgravity 2020 Sep 8;6(1):25. Epub 2020 Sep 8.

Life Support and Physical Sciences Section (TEC-MMG), European Space Agency-European Space Research and Technology Centre (ESA-ESTEC), Noordwijk, The Netherlands.

Although many examples of simulated and real microgravity demonstrating their profound effect on biological systems are described in literature, few reports deal with hypergravity and vibration effects, the levels of which are severely increased during the launch preceding the desired microgravity period. Here, we used planarians, flatworms that can regenerate any body part in a few days. Planarians are an ideal model to study the impact of launch-related hypergravity and vibration during a regenerative process in a "whole animal" context. Read More

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September 2020

Spotted fever diagnosis: Experience from a South Indian center.

Pathog Glob Health 2021 Sep 7:1-7. Epub 2021 Sep 7.

Department of Pathology, Uniformed Services University of Health Sciences, Bethesda, MD, USA.

Spotted fever (SF) is an important treatable cause of acute febrile illness (AFI) with rash and has reemerged in India. A prospective AFI with rash study was undertaken at a South Indian hospital to correlate specific clinical findings with laboratory confirmation of spotted fever. During the study period (December 2017 to May 2019), 175 patients with fever and rash were suspected to have spotted fever. Read More

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September 2021

Multiscale model of defective interfering particle replication for influenza A virus infection in animal cell culture.

PLoS Comput Biol 2021 09 7;17(9):e1009357. Epub 2021 Sep 7.

Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.

Cell culture-derived defective interfering particles (DIPs) are considered for antiviral therapy due to their ability to inhibit influenza A virus (IAV) production. DIPs contain a large internal deletion in one of their eight viral RNAs (vRNAs) rendering them replication-incompetent. However, they can propagate alongside their homologous standard virus (STV) during infection in a competition for cellular and viral resources. Read More

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September 2021

Downregulation of let-7 by Electrical Acupuncture Increases Protein Synthesis in Mice.

Front Physiol 2021 20;12:697139. Epub 2021 Aug 20.

Renal Division, Department of Medicine, Emory University, Atlanta, GA, United States.

Background: Our previous study found that acupuncture with low frequency electrical stimulation (Acu/LFES) prevents muscle atrophy by attenuation of protein degradation in mice. The current study examines the impact of Acu/LFES on protein synthesis.

Method: C57/BL6 mice received Acu/LFES treatment on hindlimb for 30 min once. Read More

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CISD2 Promotes Resistance to Sorafenib-Induced Ferroptosis by Regulating Autophagy in Hepatocellular Carcinoma.

Front Oncol 2021 16;11:657723. Epub 2021 Aug 16.

Department of General Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang, China.

Purpose: Sorafenib is a multi-kinase inhibitor that is used as a standard treatment for advanced hepatocellular carcinoma (HCC). However, the mechanism of sorafenib resistance in HCC is still unclear. It has been shown that CISD2 expression is related to the progression and poor prognosis of HCC. Read More

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ET-NET: an ensemble of transfer learning models for prediction of COVID-19 infection through chest CT-scan images.

Multimed Tools Appl 2021 Aug 31:1-20. Epub 2021 Aug 31.

Department of Computer Science & Engineering, Jadavpur University, Kolkata, 700032 India.

The COVID-19 virus has caused a worldwide pandemic, affecting numerous individuals and accounting for more than a million deaths. The countries of the world had to declare complete lockdown when the coronavirus led to community spread. Although the real-time Polymerase Chain Reaction (RT-PCR) test is the gold-standard test for COVID-19 screening, it is not satisfactorily accurate and sensitive. Read More

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SARS-CoV-2 in residential rooms of two self-isolating persons with COVID-19.

J Aerosol Sci 2022 Jan 28;159:105870. Epub 2021 Aug 28.

Department of Environmental Engineering Sciences, College of Engineering, University of Florida, Gainesville, FL, 32611, USA.

Individuals with COVID-19 are advised to self-isolate at their residences unless they require hospitalization. Persons sharing a dwelling with someone who has COVID-19 have a substantial risk of being exposed to the virus. However, environmental monitoring for the detection of virus in such settings is limited. Read More

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January 2022