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Use of MTT Assay for Proliferation of U937 Cell Line and its Inter-rater Reliability - Best Taken with a Grain of Salt.

Kathmandu Univ Med J (KUMJ) 2020 Oct.-Dec.;18(72):396-401

Department of Physiology, All India Institute of Medical Sciences (AIIMS), Bibinagar, Telangana - 508126, India.

Background Biomedical research frequently employs immortal human cell lines to study various physiological and pathological topics of interest. An important problem that is often ignored is the question of reliability of observations made while using a particular assay on cell lines, especially MTT Assay. Recent studies have questioned its reliability by highlighting the diverse intracellular environmental factors that affect accuracy of MTT assay results. Read More

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Accelerated regression-based summary statistics for discrete stochastic systems via approximate simulators.

BMC Bioinformatics 2021 Jun 23;22(1):339. Epub 2021 Jun 23.

Department of Computer Science, University of California, Santa Barbara, Santa Barbara, USA.

Background: Approximate Bayesian Computation (ABC) has become a key tool for calibrating the parameters of discrete stochastic biochemical models. For higher dimensional models and data, its performance is strongly dependent on having a representative set of summary statistics. While regression-based methods have been demonstrated to allow for the automatic construction of effective summary statistics, their reliance on first simulating a large training set creates a significant overhead when applying these methods to discrete stochastic models for which simulation is relatively expensive. Read More

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Comparison of empirical and dynamic models for HIV viral load rebound after treatment interruption.

Stat Commun Infect Dis 2020 21;12(Suppl 1). Epub 2020 Aug 21.

Department of Population Medicine, Harvard Pilgrim Health Care Institute and Harvard Medical School, Boston, MA, 02215, USA.

Objective: To compare empirical and mechanistic modeling approaches for describing HIV-1 RNA viral load trajectories after antiretroviral treatment interruption and for identifying factors that predict features of viral rebound process.

Methods: We apply and compare two modeling approaches in analysis of data from 346 participants in six AIDS Clinical Trial Group studies. From each separate analysis, we identify predictors for viral set points and delay in rebound. Read More

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Voltametric Determination of Zoledronic Acid in a Pharmaceutical Formulation

Turk J Pharm Sci 2021 06;18(3):339-343

Hikma Pharmaceuticals, Amman, Jordan

Objectives: The aim of this study is to study the electroactivity of zoledronic acid (ZOL), optimize the parameters affecting voltametric analysis of ZOL, and make a comparison between voltametric methods used to assay ZOL.

Materials And Methods: Three voltametric methods, cyclic voltammetry (CV), square wave voltammetry (SWV), and differential pulse voltammetry (DPV), were used to determine the concentrations of ZOL solutions (0.25. Read More

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[Dynamic11C-methionine PET/CT in differential diagnosis of brain gliomas].

Zh Vopr Neirokhir Im N N Burdenko 2021 ;85(3):5-13

Burdenko Center of Neurosurgery, Moscow, Russia.

Objective: To evaluate the possibilities of dynamic preoperative C-methionine (MET) PET/CT in differential diagnosis of various types of brain gliomas in adults.

Material And Methods: The study included 74 patients aged 48±14 years with supratentorial gliomas: Grade IV - glioblastoma (GB, =33), Grade III - anaplastic oligodendroglioma (AOD, =10) and anaplastic astrocytoma (AA, =12), Grade II - diffuse astrocytoma (DA, =13) and oligodendroglioma (OD, =6). All patients underwent standard MRI and dynamic MET PET/CT within 20 minutes after intravenous injection of radiopharmaceutical. Read More

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Dilemma in diagnosis and management of rare primary pleural Ewing's sarcoma with synchronous limited metastatic disease.

BMJ Case Rep 2021 Jun 21;14(6). Epub 2021 Jun 21.

Department of Surgical Oncology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, Puducherry, India.

We present a case of a 34-year-old woman who presented with complaints of fever, cough and dyspnoea of 2 months' duration. On evaluation, she was diagnosed with a rare entity primary pleural Ewing's sarcoma with synchronous metastases to mediastinal, supraclavicular nodes and single vertebra. Due to the rarity of this entity and lack of treatment guidelines on extraosseous Ewing's sarcoma, the patient was managed with a combination of multiagent chemotherapy, surgery and radiotherapy as per standard guidelines for skeletal Ewing's sarcoma. Read More

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Comparison of Blood Counts and Markers of Inflammation and Coagulation in Patients With and Without COVID-19 Presenting to the Emergency Department in Seattle, WA.

Am J Clin Pathol 2021 Jun 21. Epub 2021 Jun 21.

Departments of Laboratory Medicine and Pathology, Seattle, WA, USA.

Objectives: We compared complete blood count (CBC) with differential and markers of inflammation and coagulation in patients with and without coronavirus disease 2019 (COVID-19) presenting to emergency departments in Seattle, WA.

Methods: We reviewed laboratory values for 1 week following each COVID-19 test for adult patients who received a standard severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reverse transcription polymerase chain reaction (RT-PCR) test before April 13, 2020. Results were compared by COVID-19 status and clinical course. Read More

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RNA Network Interactions During Differentiation of Human Trophoblasts.

Front Cell Dev Biol 2021 3;9:677981. Epub 2021 Jun 3.

Department of Obstetrics, Gynecology and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States.

In the human placenta, two trophoblast cell layers separate the maternal blood from the villous basement membrane and fetal capillary endothelial cells. The inner layer, which is complete early in pregnancy and later becomes discontinuous, comprises the proliferative mononuclear cytotrophoblasts, which fuse together and differentiate to form the outer layer of multinucleated syncytiotrophoblasts. Because the syncytiotrophoblasts are responsible for key maternal-fetal exchange functions, tight regulation of this differentiation process is critical for the proper development and the functional role of the placenta. Read More

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Theragnosis for Duchenne Muscular Dystrophy.

Front Pharmacol 2021 3;12:648390. Epub 2021 Jun 3.

Laboratorio de Distrofinopatías, Cátedra de Genética, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.

Dystrophinopathies cover a spectrum of rare progressive X-linked muscle diseases, arising from mutations. They are among the most common pediatric muscular dystrophies, being Duchenne muscular dystrophy (DMD) the most severe form. Despite the fact that there is still no cure for these serious diseases, unprecedented advances are being made for the development of therapies for DMD. Read More

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Low-Cutoff Frequency Reduction in Neural Amplifiers: Analysis and Implementation in CMOS 65 nm.

Front Neurosci 2021 2;15:667846. Epub 2021 Jun 2.

Polystim Neurotech. Lab., Department of Electrical Engineering, Polytechnique Montreal, Montreal, QC, Canada.

Scaling down technology demotes the parameters of AC-coupled neural amplifiers, such as increasing the low-cutoff frequency due to the short-channel effects. To improve the low-cutoff frequency, one solution is to increase the feedback capacitors' value. This solution is not desirable, as the input capacitors have to be increased to maintain the same gain, which increases the area and decreases the input impedance of the neural amplifier. Read More

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An Extended DEIM Algorithm for Subset Selection and Class Identification.

Mach Learn 2021 Apr 21;110(4):621-650. Epub 2021 Mar 21.

Department of Pediatric Cardiology, Baylor College of Medicine, Houston, TX 77030-1892, USA.

The discrete empirical interpolation method (DEIM) has been shown to be a viable index-selection technique for identifying representative subsets in data. Having gained some popularity in reducing dimensionality of physical models involving differential equations, its use in subset-/pattern-identification tasks is not yet broadly known within the machine learning community. While it has much to offer as is, the DEIM algorithm is limited in that the number of selected indices cannot exceed the rank of the corresponding data matrix. Read More

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[Imaging of renal tumors in children].

Radiologe 2021 Jun 18. Epub 2021 Jun 18.

Klinik für Pädiatrische Onkologie und Hämatologie, Universitätskinderklinik, Universitätsklinikum des Saarlandes, Homburg, Deutschland.

Clinical/methodological Issue: Renal tumors in children are treated according to the guidelines of the Renal Tumor Study Group of the International Society of Pediatric Oncology (SIOP-RTSG). Nephroblastoma is the most frequent renal tumor in children.

Standard Radiological Methods: After sonography, magnetic resonance imaging (MRI) is the preferred imaging modality. Read More

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Tunica vaginalis testis metastasis as the first clinical manifestation of pancreatic adenocarcinoma: A case report.

World J Clin Cases 2021 Jun;9(17):4244-4252

Department of Andrology, The First Hospital of Jilin University, Changchun 130021, Jilin Province, China.

Background: Metastases from pancreas or ampullary malignancies are common, but the spread to testicle and paratesticular tissue is exceedingly rare. To the best of our knowledge, fewer than 30 cases have been reported in the literature. More rarely, metastasis to tunica vaginalis testis occurs without involvement of the testes and epididymis. Read More

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Symptomatic Os Subtibiale Following Injury: A Case Report of Failed Conservative Treatment, Leading to Differential Diagnosis Made Intraoperatively.

J Orthop Case Rep 2021 Feb;11(2):6-10

Department of Orthopaedic, Woodland Multispeciality Hospital, Kolkata, West Bengal, India.

Introduction: Os subtibiale is a rare accessory bone found adjacent to the distal tibia and is mostly asymptomatic. Differentiating it from a medial malleolar fracture is challenging. Most cases of acute ankle trauma that presents with an Os subtibiale are initially diagnosed as fractures by treating physicians at emergency centers. Read More

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February 2021

Human cultured IMR-32 neuronal-like and U87 glial-like cells have different patterns of toxicity under fluoride exposure.

PLoS One 2021 17;16(6):e0251200. Epub 2021 Jun 17.

Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém, Brazil.

Background: Fluoride (F) is a naturally exists in nature but several studies have indicated it as an environmental toxicant to all leaving beings. Human F exposure has increased over the years since this ion has been used by industry on foods, beverages, toothpastes and on water supply. Although F is safe at optimal concentrations in water supply, human exposure to high levels could trigger neurofunctional deficits. Read More

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Laboratory testing for suspected COVID-19 vaccine-induced (immune) thrombotic thrombocytopenia.

Int J Lab Hematol 2021 Jun 17. Epub 2021 Jun 17.

Department of Haematology, Sydney Centres for Thrombosis and Haemostasis, Institute of Clinical Pathology and Medical Research (ICPMR), NSW Health Pathology, Westmead, NSW, Australia.

COVID-19 (coronavirus disease 2019) represents a pandemic, and several vaccines have been produced to prevent infection and/or severe sequelae associated with SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) infection. There have been several reports of infrequent post vaccine associated thrombotic events, in particular for adenovirus-based vaccines. These have variously been termed VIPIT (vaccine-induced prothrombotic immune thrombocytopenia), VITT (vaccine-induced [immune] thrombotic thrombocytopenia), VATT (vaccine-associated [immune] thrombotic thrombocytopenia), and TTS (thrombosis with thrombocytopenia syndrome). Read More

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Rapid and simultaneous identification of three mutations by the Novaplex™ SARS-CoV-2 variants I assay kit.

J Clin Virol 2021 May 29;141:104877. Epub 2021 May 29.

Department of Infectious, Respiratory and Digestive Medicine, Graduate School of Medicine, University of the Ryukyus. 207 Uehara, Nishihara, Okinawa 903-0215, Japan.

Background: . The emergence of SARS-CoV-2 variants has caused an unexpected rebound globally. The World Health Organization has listed three variants (B. Read More

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Objective Pain Stimulation Intensity and Pain Sensation Assessment Using Machine Learning Classification and Regression Based on Electrodermal Activity.

Am J Physiol Regul Integr Comp Physiol 2021 06 16. Epub 2021 Jun 16.

University of Connecticut, Storrs, CT, United States.

An objective measure of pain remains an unmet need of people with chronic pain, estimated to be 1/3 of the adult population in the US. The current gold standard to quantify pain is highly subjective, based upon self-reporting with numerical or visual analog scales (VAS). This subjectivity complicates pain management and exacerbates the epidemic of opioid abuse. Read More

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Measurements of thermophysical properties of solid and liquid NIST SRM 316L stainless steel.

J Mater Sci 2020 ;55

Institute of Experimental Physics, Graz University of Technology, NAWI Graz, Petersgasse 16, 8010 Graz, Austria.

In this work, we perform high accuracy measurements of thermophysical properties for the National Institute of Standards and Technology standard reference material for 316L stainless steel. As these properties can be sensitive to small changes in elemental composition even within the allowed tolerances for an alloy class, by selecting a publicly available standard reference material for study our results are particularly useful for the validation of multiphysics models of industrial metal processes. An ohmic pulse-heating system was used to directly measure the electrical resistivity, enthalpy, density, and thermal expansion as functions of temperature. Read More

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January 2020

Novel dual-template molecular imprinted electrochemical sensor for simultaneous detection of CA and TPH based on peanut twin-like NiFeO/CoFeO/NCDs nanospheres: Fabrication, application and DFT theoretical study.

Biosens Bioelectron 2021 Jun 7;190:113408. Epub 2021 Jun 7.

College of Science, Sichuan Agricultural University, Xin Kang Road, Yucheng District, Ya'an, 625014, PR China. Electronic address:

Hollow peanut-shaped NiFeO/CoFeO twinned nano-spherical shell composite materials have interconnected electron channels and excellent electrochemical performance, which prompted the use of this unique spatial structure to fabricate efficient electrochemical sensors. In this work, N-doped carbon dots (NCDs) incorporated into magnetic NiFeO/CoFeO nanoparticle shell (NiFeO/CoFeO/NCDs) modified glassy carbon electrode (GCE) was applied to construct a dual-template molecularly imprinted polymer (MIP) based electrochemistry sensor (NiFeO/CoFeO/NCDs/MIP/GCE) for the simultaneous detection of catechin (CA) and theophylline (TPH). MIP was fabricated by an in-situ electrochemical polymerization strategy based on the theoretical exploration and density functional theory (DFT) computer directional simulation to screen out the optimal functional monomer (L-arginine) and the optimal ratio between the dual template molecules (CA and TPH) and functional monomer. Read More

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Improving Estimates of Neighborhood Change with Constant Tract Boundaries.

Appl Geogr 2021 Jul 24;132. Epub 2021 May 24.

U.S. Census Bureau.

Social scientists routinely rely on methods of interpolation to adjust available data to their research needs. Spatial data from different sources often are based on different geographies that need to be reconciled, and some boundaries (e.g. Read More

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Molecularly imprinted polymer-based electrochemical sensor for the determination of endocrine disruptor bisphenol-A in bovine milk.

Food Chem 2021 Jun 4;363:130287. Epub 2021 Jun 4.

REQUIMTE/LAQV, Polytechnic of Porto - School of Engineering, Rua Dr. António Bernardino de Almeida, 4249-015 Porto, Portugal.

Bisphenol A (BPA) contamination from food packaging material has been a major concern in recent years, due to its potential endocrine-disrupting effects on humans, especially infants and children. This paper reports the detection of BPA using an electrochemical sensor based on molecularly imprinted polymer (MIP). Electrochemically reduced graphene oxide coated glassy carbon electrode used for this study. Read More

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Anticoagulant-induced hemorrhagic cholecystitis with hemobilia after deceased donor kidney transplant and literature review.

Int J Surg Case Rep 2021 May 26;84:106027. Epub 2021 May 26.

Augusta University, Department of Surgery, United States of America. Electronic address:

Introduction And Importance: Hemobilia and hemorrhagic cholecystitis are uncommon causes of right upper quadrant abdominal pain. The development of intra-gallbladder and biliary bleeding has been primarily associated with abdominal trauma, malignancy, liver transplant, and iatrogenic injury to the biliary tree and vasculature. Spontaneous anticoagulant induced hemorrhagic cholecystitis and hemobilia are incredibly rare events and have only been documented by a handful of case reports. Read More

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Preoperative Multiparametric Ultrasound and Fine Needle Aspiration Cytology evaluation of parotid gland tumors: which is the best technique?

Med Ultrason 2021 Jun 10. Epub 2021 Jun 10.

Department of Radiological Sciences, Oncology and Pathology, Policlinico Umberto I "Sapienza" University of Rome, Rome, Italy.

Aims: To evaluate the pre-surgical diagnostic value of Multiparametric Ultrasound (MPUS) and Fine Needle Aspiration Cytology (FNAC) in differentiating parotid gland tumors, comparing the results with histology.

Materials And Methods: The study enrolled 84 patients with parotid gland lesions surgically treated in a single tertiary center and evaluated by MPUS. Each patient underwent FNAC. Read More

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Distinct transcriptional responses to fatal Ebola virus infection in cynomolgus and rhesus macaques suggest species-specific immune responses.

Emerg Microbes Infect 2021 Jun 11:1-48. Epub 2021 Jun 11.

Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA 92697, USA.

Ebola virus (EBOV) is a negative single-stranded RNA virus within the family and the causative agent of Ebola virus disease (EVD). Nonhuman primates (NHPs), including cynomolgus and rhesus macaques, are considered the gold standard animal model to interrogate mechanisms of EBOV pathogenesis. However, despite significant genetic similarity (>90%), NHP species display different clinical presentation following EBOV infection, notably a ∼1-2 days delay in disease progression. Read More

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Tomographic reconstruction of oxygen orbitals in lithium-rich battery materials.

Nature 2021 Jun 9;594(7862):213-216. Epub 2021 Jun 9.

Department of Mechanical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.

The electrification of heavy-duty transport and aviation will require new strategies to increase the energy density of electrode materials. The use of anionic redox represents one possible approach to meeting this ambitious target. However, questions remain regarding the validity of the O/O oxygen redox paradigm, and alternative explanations for the origin of the anionic capacity have been proposed, because the electronic orbitals associated with redox reactions cannot be measured by standard experiments. Read More

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Efficacy of behavioral audiological tests in identifying cochlear synaptopathy: a systematic review.

Eur Arch Otorhinolaryngol 2021 Jun 9. Epub 2021 Jun 9.

Department of Audiology, All India Institute of Speech and Hearing, Mysore, Karnataka, 570006, India.

Purpose: Cochlear synaptopathy or hidden hearing loss is difficult to assess due to the lack of sensitivity with standard audiological tests. Poor speech perception, especially in the presence of noise or tinnitus, is the most common complaint of these patients. The purpose of this systematic review is to identify articles in peer-reviewed journals that used behavioral measures in the effective assessment of cochlear synaptopathy or hidden hearing loss. Read More

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miR-361-5p as a promising qRT-PCR internal control for tumor and normal breast tissues.

PLoS One 2021 8;16(6):e0253009. Epub 2021 Jun 8.

Molecular Genetics Department, Biological Sciences Faculty, Tarbiat Modares University, Tehran, Iran.

Background: One of the most widely used evaluation methods in miRNA experiments is qRT-PCR. However, selecting suitable internal controls (IC) is crucial for qRT-PCR experiments. Currently, there is no consensus on the ICs for miRNA qRT-PCR experiments in breast cancer. Read More

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Challenging diagnosis of leprosy in a psychotic homeless patient with atypical clinical manifestations: an interesting case report.

BMC Infect Dis 2021 Jun 7;21(1):540. Epub 2021 Jun 7.

Department of Dermatology and Venereology, Faculty of Medicine, Universitas Padjadjaran - Dr. Hasan Sadikin General Hospital, Jl. Pasteur No. 38, Bandung, 40161, Indonesia.

Background: A decision to diagnose certain skin diseases in patient undergoing psychotic break is challenging; this includes establishing the diagnosis of leprosy. Diagnosis of leprosy is established if there is at least one of the three cardinal signs of leprosy. Histopathological examination is not a gold standard, but remains useful in atypical or clinically suspicious cases. Read More

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Semi-Quantitative RT-PCR: An Effective Method to Explore the Regulation of Gene Transcription Level Affected by Environmental Pollutants.

Fan Wang

Methods Mol Biol 2021 ;2326:95-103

School of Biological Science, Luoyang Normal University, Luoyang, China.

Semi-quantitative reverse transcription and polymerase chain reaction (sqRT-PCR) is a simple and specific method for quantitative RNA in recent years. The relative quantity of a specific mRNA in the samples can be inferred by reverse transcription of mRNA into cDNA, and PCR amplification and determination of the quantity of PCR products. The semi-quantitative analysis is carried out under a fixed number of PCR cycles, and the total RNA concentration is kept in the exponential phase of the PCR. Read More

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January 2021