14,424 results match your criteria standard chromatographic

Feature-based molecular networking for identification of organic micropollutants including metabolites by non-target analysis applied to riverbank filtration.

Anal Bioanal Chem 2021 Jul 20. Epub 2021 Jul 20.

Institute of Geology and Palaeontology - Applied Geology, University of Münster, Corrensstraße 24, 48149, Münster, Germany.

Due to growing concern about organic micropollutants and their transformation products (TP) in surface and drinking water, reliable identification of unknowns is required. Here, we demonstrate how non-target liquid chromatography (LC)-high-resolution tandem mass spectrometry (MS/MS) and the feature-based molecular networking (FBMN) workflow provide insight into water samples from four riverbank filtration sites with different redox conditions. First, FBMN prioritized and connected drinking water relevant and seasonally dependent compounds based on a modification-aware MS/MS cosine similarity. Read More

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Development and Validation of a Simple and Sensitive LC-MS/MS Method for Quantification of Metformin in Dried Blood Spot Its Application as an Indicator for Medication Adherence.

Int J Gen Med 2021 8;14:3225-3233. Epub 2021 Jul 8.

College of Pharmacy, King Saud University, Riyadh, 11671, Saudi Arabia.

Objective: Metformin (MET), an oral biguanide agent, can improve insulin resistance and decrease hepatic glucose production, leading to a reduction in blood-sugar levels. The objective of the present study was to develop and validate simple and rapid LC-MS/MS method for analysis of MET in dried blood spot (DBS) sample for patient monitoring studies purposes (drug adherence).

Methods: The chromatographic separation was achieved with Waters HSS-T3 column using gradient elution of mobile phases of two solvents: 1) solvent A, consisted of 10mM ammonium formate, 0. Read More

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Rapid organocatalytic chirality analysis of amines, amino acids, alcohols, amino alcohols and diols with achiral iso(thio)cyanate probes.

Chem Sci 2021 Jul 25;12(25):8784-8790. Epub 2021 May 25.

Department of Chemistry, Georgetown University Washington DC 20057 USA

The widespread occurrence and significance of chiral compounds does not only require new methods for their enantioselective synthesis but also efficient tools that allow rapid determination of the absolute configuration, enantiomeric composition and overall concentration of nonracemic mixtures. Although chiral analysis is a frequently encountered challenge in the chemical, environmental, materials and health sciences it is typically addressed with slow and laborious chromatographic or NMR spectroscopic techniques. We now show with almost 40 analytes representing 5 different compound classes, including mono-alcohols which are particularly challenging sensing targets, that this task can be solved very quickly by chiroptical sensing with a single, readily available arylisocyanate probe. Read More

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A Fast and Validated HPLC Method for the Simultaneous Analysis of Five 5-HT Receptor Antagonists via the Quantitative Analysis of Multicomponents by a Single Marker.

Int J Anal Chem 2021 26;2021:5533646. Epub 2021 Jun 26.

Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan, Hubei 442008, China.

In this study, a new strategy for the simultaneous quantization of five serotonin 5-hydroxytryptamine receptor antagonists-ondansetron, azasetron, ramosetron, granisetron, and tropisetron-either in infusion samples or in injection dosage form was first established based on high-performance liquid chromatography combined with a quantitative analysis of multiple components by a single marker. The quantitative analysis of multicomponents by a single marker method was conducted with ondansetron as an internal reference substance and performed using relative retention time and ultraviolet spectral similarity as the double indicator. The quantitative analysis of the 5-HT receptor antagonists was calculated and investigated based on the relative correction factors. Read More

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Isobutylene contamination of blood collected in 10-ml evacuated blood collection tubes with gray conventional rubber stoppers.

J Forensic Sci 2021 Jul 12. Epub 2021 Jul 12.

Chandler Police Department Crime Laboratory, Chandler, AZ, USA.

Dual-column headspace gas chromatographic analysis with two flame-ionization detectors is a commonly used analytical technique for forensic blood ethanol quantitation. This technique is also applicable to the identification and quantitation of other volatile organic compounds such as methanol in biological samples. Compound identification by retention time is limited to those compounds with known retention times programmed into the instrument method. Read More

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A simple LC-MS/MS method for simultaneous determination of cilostazol and ambroxol in Sprague-Dawley rat plasma and its application to drug-drug pharmacokinetic interaction study following oral delivery in rats.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 May 21;1179:122766. Epub 2021 May 21.

Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, Shenhe District, Shenyang 110016, PR China. Electronic address:

Recently, a combination of cilostazol and ambroxol has been used in the clinical treatment of stroke-associated pneumonia (SAP). However, the pharmacokinetic drug-drug interaction (DDI) of cilostazol and ambroxol has not been reported. In this paper, a rapid, reproducible and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method for simultaneous determination of cilostazol and ambroxol in Sprague-Dawley (SD) rat plasma was established and validated for the first time. Read More

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Nitrogen-doped carbon dots for dual-wavelength excitation fluorimetric assay for ratiometric determination of phosalone.

Mikrochim Acta 2021 Jul 9;188(8):247. Epub 2021 Jul 9.

Technical Center of Nanning Customs District, Nanning, 530021, China.

N-doped carbon dots (N-CDs) were fabricated in a simple procedure by hydrothermal treatment of cellobiose and urea. When excited at 235 nm or 327 nm, only one emission peak at around 420 nm has been observed. With the addition of phosalone, the excitation band at 235 nm was efficiently quenched within 1 min, while the excitation band at 327 nm showed little change. Read More

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Retention dependences support highly confident identification of lipid species in human plasma by reversed-phase UHPLC/MS.

Anal Bioanal Chem 2021 Jul 9. Epub 2021 Jul 9.

Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, Studentská 573, 53210, Pardubice, Czech Republic.

Reversed-phase ultrahigh-performance liquid chromatography-mass spectrometry (RP-UHPLC/MS) method was developed with the aim to unambiguously identify a large number of lipid species from multiple lipid classes in human plasma. The optimized RP-UHPLC/MS method employed the C18 column with sub-2-μm particles with the total run time of 25 min. The chromatographic resolution was investigated with 42 standards from 18 lipid classes. Read More

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Multi-Class UHPLC-MS/MS Method for Plant Toxins and Cyanotoxins in Food Supplements and Application for Belgian Market Samples.

Planta Med 2021 Jul 9. Epub 2021 Jul 9.

Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Technology and Food Science Unit, Melle, Belgium.

The presence of plant toxins and/or cyanotoxins in food supplements implies consumer health risks. Therefore, a targeted ultra-high performance liquid chromatographic-tandem mass spectrometric method to detect/quantify 25 toxins simultaneously in food supplement formulations was developed and validated. Full validation for tablets/powders and secondary validation for a liquid and soft gel capsule indicated that most compounds were efficiently extracted (≥ 75%), while others were only partly extracted (18 - 61%). Read More

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An UPLC - Q - Orbitrap method for pharmacokinetics and tissue distribution of four triterpenoids in rats after oral administration of Poria cocos ethanol extracts.

J Pharm Biomed Anal 2021 Sep 2;203:114237. Epub 2021 Jul 2.

School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, China; Institute of Pharmaceutics, Anhui Academy of Chinese Medicine, Hefei, 230012, China; Engineering Technology Research Center of Modernized Pharmaceutics, Education Office of Anhui Province, Hefei, 230012, China; Anhui Province Key Laboratory of Chinese Medicinal Formula, Hefei, 230012, China; Synergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement, Hefei, 230012, China; Anhui Province Key Laboratory of Pharmaceutical Preparation Technology and Application, Hefei, 230012, China. Electronic address:

Poria cocos (Schw.) Wolf, is a fungus that is widely used as medicine and dietary supplement in China. But its action mechanism is still not very clear. Read More

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September 2021

Simple, Axial Ligand-Mediated Route to Water-Soluble Iridium Corroles.

ACS Omega 2021 Jun 15;6(25):16683-16687. Epub 2021 Jun 15.

Department of Chemistry, UiT-The Arctic University of Norway, N-9037 Tromsø, Norway.

The synthesis and purification of water-soluble porphyrin-type compounds for photodynamic therapy and other medical applications is often a tedious exercise. Here, we have investigated the simple stratagem of adding a water-soluble axial ligand to the standard protocol for iridium insertion into simple -triarylcorroles. Early results showed that six-coordinate Ir[TXPC](dna) derivatives, in which TXPC = tris(-X-phenyl)corrole (X = CF, CN, H, and OMe) and dna = dinicotinic acid, are highly water-soluble. Read More

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Cross-linked γ-cyclodextrin metal-organic framework-a new stationary phase for the separations of benzene series and polycyclic aromatic hydrocarbons.

Mikrochim Acta 2021 Jul 6;188(8):245. Epub 2021 Jul 6.

School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang, Liaoning Province, People's Republic of China.

The cross-linked γ-cyclodextrin metal-organic framework (CL-CD-MOF) was synthesized by crosslinking γ-cyclodextrin metal-organic framework (γ-CD-MOF) with diphenyl carbonate to separate benzene series and polycyclic aromatic hydrocarbons (PAHs). The separation ability of the CL-CD-MOF packed column was assessed in both reverse-phase (RP-) and normal-phase (NP-) modes. The retention mechanisms of these compounds were discussed and confirmed by combining molecular simulations in detail. Read More

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Development of a Validated Bioanalytical UPLC-MS/MS Method for Quantification of Neratinib: A Recent Application to Pharmacokinetic Studies in Rat Plasma.

J Chromatogr Sci 2021 Jul 6. Epub 2021 Jul 6.

Department of Pharmaceutical Sciences, Shalom Institute of Health & Allied Sciences, Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad 110062, India.

Neratinib, a tyrosine kinase inhibitor, was very recently approved by USFDA in 2017 as an anticancer drug to treat of HER2 positive breast cancers. The present work provides an account on the development of a validated bioanalytical UPLC-MS/MS method for quantification of neratinib and internal standard (imatinib) in rat plasma and tissue homogenates. A UPLC having a 100 mm C18 column (1. Read More

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High-sensitivity methods for cardiac troponins: The mission is not over yet.

Adv Clin Chem 2021 9;103:215-252. Epub 2020 Oct 9.

Dipartimento di Medicina di Laboratorio, Azienda Ospedaliera Universitaria di Padova, Padova, Italy; Dipartimento di Medicina, Università di Padova, Padova, Italy.

The measurement of cardiac troponin (cTn) is recommended by all guidelines as the gold standard for the differential diagnosis of Acute Coronary Syndromes. The aim of this review is to discuss in details some key issues regarding both analytical and clinical characteristics of the high-sensitivity methods for cTn (hs-cTn), which are still considered controversial or unresolved. In particular, the major clinical concern regarding hs-cTn methods is the difficulty to differentiate the pathophysiological mechanism responsible for biomarker release from cardiomyocytes after reversible or irreversible injury, respectively. Read More

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October 2020

Validation of Analytical Methods for Tacrolimus Determination in Poly(-caprolactone) Nanocapsules and Identification of Drug Degradation Products.

J Nanosci Nanotechnol 2021 12;21(12):5920-5928

Department of Pharmaceutical Sciences, State University of Ponta Grossa, Ponta Grossa - PR, 84010-330, Brazil.

The aim of this paper was to use chromatographic tools for validating an analytical method for the tacrolimus (TAC) determination in polymeric nanocapsules and for identifying the drug degradation products after alkaline stress. A rapid Ultra-High-Performance Liquid Chromatography coupled with photo-diode array (UHPLC-PDA) method was successfully performed using the following chromatographic conditions: the Shimadzu Shim-pack XR-ODS III C18 column (100 mm×2.00 mm, 2. Read More

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December 2021

Recent Advances in Electrochemical Determination of Pesticides.

J Nanosci Nanotechnol 2021 12;21(12):5795-5811

Faculty of Science and Mathematics, University of Niš, Niš 18000, Serbia.

Widespread usage of pesticides in agricultural practice caused their residues to appear in water and food products intended for human consumption. The potential toxicity of these resources has raised awareness about pesticide tracking in the environment. Development of reliable electrochemical sensors for the on-site determination of pesticide concentrations is envisioned as an alternative to conventional chromatographic methods which are robust, expensive and require skilled work force. Read More

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December 2021

[Simultaneous determination of 22 sunscreen agents in cosmetics by ultra-high-performance liquid chromatography using diode array detector].

Se Pu 2021 Apr;39(4):415-423

Guangxi Institute for Food and Drug Control, Nanning 530021, China.

A fast and simple ultra-high-performance liquid chromatography using diode array detector (UHPLC-DAD) technique has been developed for the simultaneous determination of 22 sunscreen agents (UV filters), viz. phenylbenzimidazole sulfonic acid (PBS), terephthalydene dicamphor sulfonic acid (TDS), benzophenone-4 (BZ4), camphor benzalkonium methosulfate (CBM), benzophenone-2 (BZ2), benzylidene camphor sulfonic acid (BCS), benzophenone-3 (BZ3), 3-benzylidene camphor (3BC), isoamyl -methoxycinnamate (IMC), 4-methylbenzylidene camphor (MBC), diethylamino hydroxybenzoyl hexyl benzoate (DHHB), octocrylene (OCR), butyl methoxydibenzoyl methane (BDM), ethylhexyl dimethyl PABA (EDP), ethylhexyl methoxycinnamate (EMC), homosalate (HS), ethylhexyl salicylate (ES), diethylhexyl butamido triazone (DBT), ethylhexyl triazone (ET), drometrizole trisiloxane (DRT), methylene bis-benzotriazolyl tetramethylbutylphenol (MBP), and bis-ethylhexyloxyphenol methoxyphenyl triazine (EMT) in cosmetic products. Different parameters, such as column type, oven temperature, mobile phase composition, and detection wavelength, were studied. Read More

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[Simultaneous determination of 11 prohibited and restricted veterinary drugs and their metabolites in animal-derived foods by ultra performance liquid chromatography-tandem mass spectrometry coupled with solid phase extraction].

Se Pu 2021 Apr;39(4):406-414

Anhui Provincial Center for Disease Control and Prevention, Hefei 230601, China.

Chloramphenicols, nitroimidazoles, lincosamides, and macrolides are common antibiotics used in veterinary medicine. Overdoses of these drugs will lead to residual substances in animal-derived foods and accumulate in the body through the food chain, thereby exerting adverse effects on human health. Therefore, regulation of veterinary drug levels is imperative to ensure the quality of animal-derived foods and safeguard the health of consumers. Read More

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[Simultaneous determination of 12 lipophilic shellfish toxins in plasma and urine by ultra-high performance liquid chromatography-tandem mass spectrometry].

Se Pu 2021 Apr;39(4):399-405

Beijing Center for Disease Control and Prevention, Beijing 100013, China.

Lipophilic shellfish toxins pose significant threats to the health of seafood consumers and public health. The symptoms of these kinds of toxins include severe diarrhea, abdominal cramps, nausea and gastrointestinal disorders. These symptoms could be hardly distinguished with many other symptoms of food poisoning and diseases. Read More

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[Recent advances in microchip liquid chromatography].

Se Pu 2021 Apr;39(4):357-367

College of Chemistry & Chemical Engineering, Xiamen University, Xiamen 361005, China.

Miniaturization is an important trend in modern analytical instrument development, including miniaturized gas chromatography and liquid chromatography, as well as micro bore columns and capillary-to-microfluidics-based platforms. Apart from the miniaturization of the separation column, which is the core part of a chromatographic system, other parts of the system, including the sampler, pumping system, gradient generation, and detection systems, have been miniaturized. Miniaturized liquid chromatography significantly reduces solvent and sample consumption while providing comparable or even better separation efficiency. Read More

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[Determination of eight organic residues in ion exchange resins by headspace gas chromatography].

Se Pu 2021 Jul;39(7):764-770

National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China.

An analytical method based on headspace gas chromatography was developed for the determination of eight organic residues in ion exchange resins, methyl isopropyl ketone, methyl butyrate, 3-pentanone, 1,3-diethyl benzene, 1,4-diethyl benzene, dichloroethane, 1,3-dichlorobenzene, and methyl methacrylate. The organic residues in different types of resins were studied to provide a basis for the safe use of ion-exchange resins in food and medicine. The main factors (chromatographic column, equilibrium temperature, equilibrium time, flow rate, etc. Read More

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[Simultaneous determination of six haloacetonitriles in finished water for drinking by purge and trap-gas chromatography-triple quadrupole mass spectrometry].

Se Pu 2021 Jul;39(7):758-763

Beijing Center for Disease Prevention and Control, Beijing Research Center for Preventive Medicine, Beijing 100013, China.

Haloacetonitriles (HANs) are widely used in finished water as unregulated disinfection by-products. HANs may pose much threat to human health, and there is no relevant standard examination method for these compounds. A method was established for the simultaneous determination of six HANs (chloroacetonitrile (CAN), dichloroacetonitrile (DCAN), trichloroacetonitrile (TCAN), bromoacetonitrile (BAN), bromochloroacetonitrile (BCAN), and dibromoacetonitrile (DBAN)) in finished water by using purge and trap-gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS). Read More

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[Determination of hydrazine in prednisolone by derivatization-gas chromatography-triple quadrupole mass spectrometry].

Se Pu 2021 Jul;39(7):750-757

Beijing Key Laboratory of Organic Materials Testing Technology & Quality Evaluation Beijing Centre for Physical and Chemical Analysis, Beijing 100089, China.

Prednisolone is an adrenal glucocorticoid drug with immunosuppressive, anti-inflammatory, anti-allergic, and antiviral effects that are widely exploited in clinical treatment. The hydrazine residue to prednisolone directly affects medication safety and threatens the patient's health. At present, there are no relevant laws, regulations, and standards to control the residual limit of hydrazine in drugs at home or abroad. Read More

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[Simultaneous determination of pentostatin and 2'-amino-2'-deoxyadenosine in fermentation broth by high performance liquid chromatography-tandem mass spectrometry].

Se Pu 2021 Jul;39(7):744-749

Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China.

An analytical method was established for the simultaneously determination the pentostatin and 2'-amino-2'-deoxyadenosine contents in fermentation broth by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). After high-speed centrifugation, aqueous solution dilution, vortex shock, and microfiltration, the fermentation broth samples were analyzed by HPLC-MS/MS. The samples were separated on a Waters Atlantis T3 column (100 mm×2. Read More

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[Simultaneous determination of three quaternary ammonium muscle relaxants in blood by high performance liquid chromatography-tandem mass spectrometry].

Se Pu 2021 Jul;39(7):695-701

State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China.

Vecuronium, rocuronium, and pancuronium are widely used as non-depolarizing muscle relaxants. There have been occasional cases of allergic reactions and even death when using such muscle relaxants. Rapid determination of the concentration of these muscle relaxants in blood can provide valuable information for early clinical diagnosis. Read More

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[Research progress on analytical methods for the determination of hexachlorobutadiene].

Se Pu 2021 Jan;39(1):46-56

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.

Hexachlorobutadiene (HCBD) is one of persistent organic pollutants (POPs) listed in Annex A and Annex C of the Stockholm Convention in 2015 and 2017, respectively. Research on the sources, environmental occurrences, and biological effects of HCBD has a great significance in controlling this newly added POPs. Sensitive and credible methods for the determination of HCBD are preconditions and form the basis for related research work. Read More

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January 2021

[Advances in high-throughput proteomic analysis].

Se Pu 2021 Feb;39(2):112-117

Department of Chemistry, School of Science, Southern University of Science and Technology, Shenzhen 518055, China.

Proteomic analysis aims at characterizing proteins on a large scale, including their relative abundance, post-translational modifications, protein-protein interactions and so on. Proteomic profiling helps to elucidate the mechanisms of disease occurrence and to discover new diagnostic markers and therapeutic targets. Mass spectrometry (MS)-based proteomic technologies have advanced to allow comprehensive qualitative and quantitative proteome profiling across a myriad proteins in cells and tissues. Read More

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February 2021

[Determination of 13 sunscreen agents in cosmetics by gas chromatography-mass spectrometry].

Se Pu 2021 May;39(5):552-557

Hubei Institute for Drug Control, Wuhan 430075, China.

Sunscreens can be categorized as physical and chemical types. Chemical sunscreens are widely used in cosmetics, and hence, their concentration in the desired products should be strictly monitored. Gas chromatography-mass spectrometry (GC-MS) is widely used for the analysis of cosmetics as it does not require organic mobile phases and allows for accurate qualitative and quantitative analyses. Read More

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[Design and application of special solid phase extraction column for three cannabinol compounds in hemp].

Se Pu 2021 May;39(5):534-540

Center of Analytical and Testing, Qiqihar University, Qiqihar 161006, China.

Cannabidiol (CBD), cannabinol (CBN), and Δ9-tetrahydrocannabinol (THC) are the most important components of hemp, whose concentrations determine the properties and applications of hemp. Hemp contains a large number of impurities, which must be removed from the extracting solution before determining the cannabinol contents by ultra-high performance liquid chromatography (UHPLC). Neutral alumina, magnesium silicate, and graphitized carbon black have different surface characteristics when used as adsorbents. Read More

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[Determination of nine ginsenosides in health foods by solid extraction phase-ultra performance liquid chromatography-tandem mass spectrometry].

Se Pu 2021 May;39(5):526-533

Guangdong Medical University, Dongguan 523808, China.

Ginsenosides are the main active compounds of ginseng, American ginseng and Panax notoginseng. They have certain pharmacological effects on the cardiovascular, immune and central nervous systems. Most ginsenosides are naturally classified as protopanaxatriol (PPT), protopanaxadiol (PPD), and oleanolic acid (OA) according to their aglycone skeletons. Read More

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