8,635 results match your criteria staining employed

Acute benzo[a]pyrene exposure induced oxidative stress, neurotoxicity and epigenetic change in blood clam Tegillarca granosa.

Sci Rep 2021 Sep 21;11(1):18744. Epub 2021 Sep 21.

National Engineering Research Center of Marine Facilities Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, 316004, Zhejiang, China.

The blood clam (Tegillarca granosa) is being developed into a model bivalve mollusc for assessing and monitoring marine pollution on the offshore seabed. However, the information on the response of blood clam to PAHs, an organic pollutant usually deposited in submarine sediment, remains limited. Herein, we employed multiple biomarkers, including histological changes, oxidative stress, neurotoxicity and global DNA methylation, to investigate the effects of 10 and 100 μg/L Bap exposure on the blood clams under laboratory conditions, as well as the potential mechanisms. Read More

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September 2021

Low-dose cyclooxygenase-2 (COX-2) inhibitor celecoxib plays a protective role in the rat model of neonatal necrotizing enterocolitis.

Ling Sun

Bioengineered 2021 Dec;12(1):7234-7245

Neonatal Intensive Care Unit, Yantaishan Hospital, Yantai, China.

This study aims to investigate the effects of the cyclooxygenase-2 (COX-2) inhibitor celecoxib on neonatal necrotizing enterocolitis (NEC) in rats. After treatment with a low dose of celecoxib (0.5, 1, or 1. Read More

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December 2021

Endoplasmic Reticulum Stress Is Associated with the Mesencephalic Dopaminergic Neuron Injury in Stressed Rats.

Anal Cell Pathol (Amst) 2021 8;2021:7852710. Epub 2021 Sep 8.

Institute of Forensic Science, Ministry of Public Security People's Republic of China, No. 17 Nanli Mulidi, Beijing 100038, China.

An increasing number of people are in a state of stress due to social and psychological pressures, which may result in mental disorders. Previous studies indicated that mesencephalic dopaminergic neurons are associated with not only reward-related behaviors but also with stress-induced mental disorders. To explore the effect of stress on dopaminergic neuron and potential mechanism, we established stressed rat models of different time durations and observed pathological changes in dopaminergic neurons of the ventral tegmental area (VTA) through HE and thionine staining. Read More

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September 2021

Quercetin alleviates chronic renal failure by targeting the PI3k/Akt pathway.

Bioengineered 2021 Dec;12(1):6538-6558

Division of Nephrology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong, Guangzhou, China.

Chronic renal failure (CRF) threatens human health greatly and attracts worldwide concerns of health professionals in the public health sector. In our preliminary study, we found that Compound capsule (Shengqing Jiangzhuo Capsule, SQJZJN) had a significant therapeutic effect on CRF. Quercetin is one of the main components of this Compound capsule. Read More

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December 2021

The Specific NLRP3 Antagonist IFM-514 Decreases Fibrosis and Inflammation in Experimental Murine Non-Alcoholic Steatohepatitis.

Front Mol Biosci 2021 13;8:715765. Epub 2021 Aug 13.

Translational Hepatology, Department of Internal Medicine I, Universitätsklinikum/ Goethe-Universität, Frankfurt, Germany.

Activation of the inflammasome NLRP3 (NOD-, LRR- and pyrin domain containing 3) contributes to the development of non-alcoholic fatty liver disease (NAFLD) and progression to non-alcoholic steatohepatitis (NASH). Therefore, this study explored the therapeutic effects of a novel and selective NLRP3 antagonist in a murine dietary model of NASH. Groups of 12-week-old mice were fed ad lib for 7 weeks with a methionine/choline deficient (MCD) and western diet (WD). Read More

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Study on the Effect of Macrophages on Vascular Endothelium in Mice With Different TCM Syndromes of Dyslipidemia and its Biological Basis Based on RNA-Seq Technology.

Front Pharmacol 2021 26;12:665635. Epub 2021 Aug 26.

School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.

"Treating the same disease with different methods" is a Traditional Chinese medicine (TCM) therapeutic concept suggesting that, while patients may be diagnosed with the same disease, they may also have different syndromes that require distinct drug administrations. This study aimed to identify the differentially expressed genes and related biological processes in dyslipidemia in relation to phlegm-dampness retention (PDR) syndrome and spleen and kidney Yang deficiency (SKYD) syndrome using transcriptomic analysis. Ten ApoE mice were used for the establishment of dyslipidemic disease-syndrome models via multifactor-hybrid modeling, with five in the PDR group and five in the SKYD group. Read More

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Elemene Nanoemulsion Inhibits Metastasis of Breast Cancer by ROS Scavenging.

Int J Nanomedicine 2021 31;16:6035-6048. Epub 2021 Aug 31.

Department of Biomedical Engineering, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, People's Republic of China.

Introduction: Elemene (CH) is a sesquiterpene compound extracted from the rhizome of herbs. In the past decades, the anti-tumor activity of elemene has been observed in vitro and in some clinical practices. However, pharmacological mechanisms of elemene are not demonstrated adequately, which may lead to improper clinical applications. Read More

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GATA3 improves the protective effects of bone marrow-derived mesenchymal stem cells against ischemic stroke induced injury by regulating autophagy through CREG.

Brain Res Bull 2021 Sep 6;176:151-160. Epub 2021 Sep 6.

Department of Neurology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, China. Electronic address:

Background: Bone marrow-derived mesenchymal stem cells (BMSCs) transplantation has been demonstrated to benefit functional recovery after ischemic stroke, however, the low survival rate of BMSCs in ischemic microenvironment largely limits its use.

Methods: Rat BMSCs (rBMSCs) were isolated from SD rats and treated with oxygen glucose deprivation/reoxygenation (OGD) to mimic ischemic microenvironment in vitro. Expression of mRNAs and proteins were assessed by qRT-PCR and western blot, respectively. Read More

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September 2021

Resveratrol Alleviates Dextran Sulfate Sodium-Induced Acute Ulcerative Colitis in Mice by Mediating PI3K/Akt/VEGFA Pathway.

Front Pharmacol 2021 23;12:693982. Epub 2021 Aug 23.

Department of Pharmacy, The First People's Hospital of Yongkang Affiliated to Hangzhou Medical College, Jinhua, China.

Ulcerative colitis (UC) is a chronic inflammatory disease that affects the colon, and its incidence is on the rise worldwide. Resveratrol (RSV), a polyphenolic compound, was recently indicated to exert anti-inflammatory effects on UC. Consequently, the current study was conducted to investigate the mechanism of RSV on alleviating UC in mice by mediating intestinal microflora homeostasis. Read More

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Improving the quality of malaria diagnosis in southern Africa through the development of a regional malaria slide bank.

Malar J 2021 Sep 8;20(1):365. Epub 2021 Sep 8.

Centre for Emerging Zoonotic and Parasitic Infections, National Institute for Communicable Diseases, Johannesburg, South Africa.

Background: A malaria slide bank (MSB) is a useful asset for any malaria microscopy testing laboratory to have access to. However, it is not feasible for every country to have its own MSB. If countries are able to pool their resources, a regional MSB is a viable solution. Read More

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September 2021

Ferulic acid improves motor function induced by spinal cord injury in rats via inhibiting neuroinflammation and apoptosis.

Acta Cir Bras 2021 3;36(7):e360705. Epub 2021 Sep 3.

MM. Department of Pharmacy - Zhejiang Pharmaceutical College - Ningbo, China.

Purpose: To investigate the effect of ferulic acid (FA) on spinal cord injury (SCI)-induced motor dysfunction and to explore the possible pharmacological mechanisms.

Methods: Adult male Wistar rats were used in our study. SCI was achieved by clipping the spinal cord T9 of the rat by a vascular clip for 2 minutes. Read More

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September 2021

[Effects of Osthole on Apoptosis and Autophagy of Human Tongue Cancer Tca8113 Cells].

Xing-Ya Sun Xin Liu

Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2021 Aug;43(4):501-506

Department of Laboratory Medicine,The First Affiliated Hospital of Jinzhou Medical University,Jinzhou,Liaoning 121000,China.

Objective To investigate the effects of osthole on the proliferation,apoptosis,and autophagy of human tongue cancer Tca8113 cells and explore its possible mechanism of action. Methods Tca8113 cells were cultured and divided into a control group without drugs and the experimental groups with 40,80,120,and 160 μmol/L osthole.The inhibitory effect of osthole on the proliferation of Tca8113 cells was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and colony formation assay. Read More

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Evaluation of NTRK Gene Fusion by Five Different Platforms in Triple-Negative Breast Carcinoma.

Front Mol Biosci 2021 19;8:654387. Epub 2021 Aug 19.

Department of Pathology, State Key Laboratory of Complex Severe and Rare Disease, Molecular Pathology Research Center, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Triple-negative breast carcinoma (TNBC) is an aggressive disease that has a poor prognosis since it lacks effective treatment methods. Neurotrophic tyrosine receptor kinase (NTRK) fusion genes are excellent candidates for targeted RTK inhibitor therapies and there are available targeted therapy drugs for the treatment of TRK fusion-positive tumors in a tumor agnostic pattern. Our study was designed to investigate the NTRK gene fusion status in TNBC patients and to determine whether RTK-targeted therapies are suitable for TNBC patients. Read More

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Serum Anti-PDLIM1 Autoantibody as Diagnostic Marker in Ovarian Cancer.

Front Immunol 2021 19;12:698312. Epub 2021 Aug 19.

Henan Institute of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, China.

Background: Serum autoantibodies (AAbs) against tumor-associated antigens (TAAs) could be useful biomarkers for cancer detection. This study aims to evaluate the diagnostic value of autoantibody against PDLIM1 for improving the detection of ovarian cancer (OC).

Methods: Immunohistochemistry (IHC) test in tissue array containing 280 OC tissues, 20 adjacent tissues, and 8 normal ovarian tissues was performed to analyze the expression of PDLIM1 in tissues. Read More

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SP600125 restored tumor necrosis factor-α-induced impaired chondrogenesis in bone mesenchymal stem cells and its anti-osteoarthritis effect in mice.

Stem Cells Dev 2021 Sep 5. Epub 2021 Sep 5.

University of South China, 34706, Hengyang, Hunan, China;

Inflammation, the main factor in the progression of osteoarthritis (OA), impairs the chondrogenesis of bone mesenchymal stem cells (BMSCs), which is an appealing process to target to regenerate impaired articular cartilage. This article aimed to investigate whether SP600125, a competitive ATP-specific inhibitor of the JNK pathway, could promote the chondrogenesis of BMSCs by enhancing their anti-inflammatory capacity. Chondrogenic differentiation was assessed by Alcian blue staining, immunofluorescence staining and western blot. Read More

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September 2021

MiR-874-3p inhibits osteogenic differentiation of human periodontal ligament fibroblasts through regulating Wnt/β-catenin pathway.

J Dent Sci 2021 Oct 7;16(4):1146-1153. Epub 2021 Apr 7.

Department of Oral Restoration, Guangdong Stomatological Hospital, Guangzhou, Guangdong, China.

Background/purpose: Previous studies have shown that miR-874 is considered to be an important regulatory factor that participated in osteoclast differentiation. The role of miR-874-3p on osteoclast differentiation of human periodontal ligament fibroblast(hPDLF), however, is still unclear. This study was aimed to delve into the related molecular mechanism of miR-874-3p on hPDLF osteoclast differentiation. Read More

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October 2021

In-Depth Profiling of T-Cell Responsiveness to Commonly Recognized CMV Antigens in Older People Reveals Important Sex Differences.

Front Immunol 2021 13;12:707830. Epub 2021 Aug 13.

Department of Clinical and Experimental Medicine, Brighton and Sussex Medical School, Brighton, United Kingdom.

The impact of biological sex on T-cell immunity to Cytomegalovirus (CMV) has not been investigated in detail with only one published study comparing CMV-specific T-cell responses in men and women. Many studies, however, have shown an association between CMV infection and immunosenescence, with broad effects on peripheral blood lymphocyte subsets as well as the T and B-cell repertoires. Here, we provide a detailed analysis of CMV-specific T-cell responses in (n=94) CMV+ older people, including 47 women and 47 men aged between 60 and 93 years. Read More

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Downregulation of Salusin-β protects renal tubular epithelial cells against high glucose-induced inflammation, oxidative stress, apoptosis and lipid accumulation via suppressing miR-155-5p.

Bioengineered 2021 Dec;12(1):6155-6165

Department of Endocrinology, Zhejiang Xiaoshan Hospital, Hangzhou, Zhejiang Province, China.

Diabetic nephropathy (DN) is the main contributor to the excess mortality for patients suffering from diabetes. Here, C57BL/6 mice received 4 weeks of high-fat diet and intraperitoneal injection of STZ (100 mg/kg). Mice with random blood glucose level ≥16. Read More

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December 2021

PD-L1 expression in megakaryocytes and its clinicopathological features in primary myelofibrosis patients.

J Pathol Clin Res 2021 Sep 4. Epub 2021 Sep 4.

Division of Hematology, Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan.

Myeloproliferative neoplasms (MPNs) are characterized by upregulation of proinflammatory cytokines and immune dysregulation, which provide a reasonable basis for immunotherapy in patients. Megakaryocytes are crucial in the pathogenesis of primary myelofibrosis (PMF), the most clinically aggressive subtype of MPN. In this study, we aimed to explore PD-L1 (programmed death-ligand 1) expression in megakaryocytes and its clinical implications in PMF. Read More

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September 2021

Diagnosis of NUT Carcinoma Despite False-Negative Next-Generation Sequencing Results: A Case Report and Literature Review.

Onco Targets Ther 2021 27;14:4621-4633. Epub 2021 Aug 27.

Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Shenyang, 110001, People's Republic of China.

Nuclear protein in testis (NUT) carcinoma (NC) is a poorly differentiated malignant tumor with a poor prognosis, which is caused by the gene rearrangement. Positive staining of NUT using immunohistochemistry (IHC) or gene rearrangement of revealed by genetic analysis, such as fluorescence in situ hybridization (FISH) or next-generation sequencing (NGS), are important strategies used for accurate diagnosis. In the current study, we present a case of NC in an 18-year-old man who had a chief complaint of nasal congestion, nasal bleeding, and anosmia. Read More

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[Role of Puerariae Lobatae Radix in Gegen Decoction for treatment of primary dysmenorrhea].

Zhongguo Zhong Yao Za Zhi 2021 Aug;46(15):3926-3933

Department of Pharmacology of Chinese Materia Medica, School of Traditional Chinese Pharmacy, China Pharmaceutical University Nanjing 211198, China Jiangsu Key Laboratory of Traditional Chinese Medicine Evaluation and Translational Research, China Pharmaceutical University Nanjing 211198, China.

This study aimed to explore the characteristic role of Puerariae Lobatae Radix(PLR) in Gegen Decoction for the treatment of primary dysmenorrhea(PD). Estrogen(E_2) was combined with oxytocin to establish a mouse model of PD. The mice were randomly divided into a normal group, a model group, a Gegen Decoction group, a PLR-free Gegen Decoction group, a PLR group, and a positive drug group(ibuprofen). Read More

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[Qingre Xiaoyanning Capsules alleviate HSV-1 susceptibility induced by emotional stress].

Zhongguo Zhong Yao Za Zhi 2021 Aug;46(16):4208-4213

Guangdong Engineering Research Center of Chinese Medicine & Disease Susceptibility, Jinan University Guangzhou 510632,China College of Pharmacy, Jinan University Guangzhou 510632, China School of Traditional Chinese Medicine,Jinan University Guangzhou 510632, China Integrated Chinese and Western Medicine Postdoctoral Research Station,Jinan University Guangzhou 510632, China.

In this study, emotional stress-induced herpes simplex virus type 1(HSV-1) susceptibility model was employed to simu-late the pathological state of " depression-induced liver fire", and the protection effect of Qingre Xiaoyanning(QX) in clearing liver fire was investigated. BALB/c mice were randomly divided into a normal group, a HSV-1 group, a restraint stress + HSV-1 group,low-(0. 658 g·kg~(-1)) and high-dose(1. Read More

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High Immunoexpression of COX-2 as a Metastatic Risk Factor in ccRCC without PD-L1 Involvement.

Res Rep Urol 2021 24;13:623-630. Epub 2021 Aug 24.

Department of Anatomical Pathology, Faculty of Medicine, Universitas Padjadjaran/Dr. Hasan Sadikin Hospital, Bandung, Indonesia.

Introduction: Clear cell renal cell carcinoma (ccRCC) is the most lethal type of malignancy of the urinary tract system as it is resistant to chemotherapy and radiation and has a survival rate of less than 5% in cases of metastasis. Inflammation plays an essential role in the metastasis of ccRCC. Cyclooxygenase-2 (COX-2) is an inflammatory protein that affects the processes of carcinogenesis, invasion, migration, metastasis, and angiogenesis. Read More

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attenuates osteoarthritic pain by inhibiting the -mediated / signalling axis.

Bone Joint Res 2021 Aug;10(8):548-557

Teaching and Research Section of Clinical Nursing, Xiangya Hospital of Central South University, Changsha, China.

Aims: MicroRNA-183 () is known to play important roles in osteoarthritis (OA) pain. The aims of this study were to explore the specific functions of in OA pain and to investigate the underlying mechanisms.

Methods: Clinical samples were collected from patients with OA, and a mouse model of OA pain was constructed by surgically induced destabilization of the medial meniscus (DMM). Read More

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Knockdown of lncRNA XIST inhibited apoptosis and inflammation in renal fibrosis via microRNA-19b-mediated downregulation of SOX6.

Mol Immunol 2021 Aug 27;139:87-96. Epub 2021 Aug 27.

Department of Urology, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, PR China. Electronic address:

Background: Kidney damage often develops into renal fibrosis. Apoptosis and inflammatory response are the main factors driving the process of renal fibrosis. Here we showed that lncRNA XIST/ miR-19b / SOX6 signal axis regulated apoptosis and inflammation of renal fibrosis. Read More

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3D Magnetic Particle Imaging of Human Stem Cell-Derived Islet Organoid Transplantation Using a Machine Learning Algorithm.

Front Cell Dev Biol 2021 12;9:704483. Epub 2021 Aug 12.

Precision Health Program, Michigan State University, East Lansing, MI, United States.

Stem cell-derived islet organoids constitute a promising treatment of type 1 diabetes. A major hurdle in the field is the lack of appropriate method to determine graft outcome. Here, we investigate the feasibility of tracking of transplanted stem cell-derived islet organoids using magnetic particle imaging (MPI) in a mouse model. Read More

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Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Improve Ovarian Function and Proliferation of Premature Ovarian Insufficiency by Regulating the Hippo Signaling Pathway.

Front Endocrinol (Lausanne) 2021 12;12:711902. Epub 2021 Aug 12.

Department of Obstetrics and Gynecology, The Second Hospital of Hebei Medical University, Shijiazhuang, China.

Background: Premature ovarian insufficiency (POI) is associated with severe physical damage and psychological burden on women. Transplantation of exosomes is an encouraging regenerative medicine method, which has the potential for restoring ovarian functions on POI with high efficiency. This study aims at evaluating the therapeutic efficacy of human umbilical cord mesenchymal stem cell-derived exosomes (hUCMSC-Exos) on ovarian dysfunction of POI and the role of Hippo pathway in this exosome-mediated treatment. Read More

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Preclinical Characterization of a Novel Anti-Cancer PD-L1 Inhibitor RPH-120.

Front Pharmacol 2021 11;12:723038. Epub 2021 Aug 11.

R-Pharm Overseas Inc, San Diego, CA, United States.

RPH-120 is a novel fully human anti-PD-L1 IgG1 monoclonal antibody with specifically designed Asn300Ala mutation in Fc fragment. Surface plasmon resonance assay showed that affinity of the RPH-120 to the dimeric form of human PD-L1-Fc fusion protein was much higher than affinity to the monomeric His-tagged PD-L1. Further binding studies demonstrated that RPH-120 is able to bind to human and monkey but not mouse PD-L1. Read More

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Fuzhenghefuzhiyang Formula (FZHFZY) Improves Epidermal Differentiation Suppression of the Akt/mTORC1/S6K1 Signalling Pathway in Psoriatic Models.

Front Pharmacol 2021 11;12:650816. Epub 2021 Aug 11.

State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine), Guangzhou, China.

Psoriasis is a chronic proliferative skin disorder characterised by abnormal epidermal differentiation. The Fuzhenghefuzhiyang (FZHFZY) formula created by Chuanjian Lu, a master of Chinese medicine in dermatology, has been external used in the Guangdong Provincial Hospital of Chinese Medicine for the treatment of psoriasis, but its mechanisms of action against psoriasis remain poorly understood. This study involved an exploration of the effects of FZHFZY on epidermal differentiation and its underlying mechanisms in interleukin (IL)-17A/IL-22/interferon (IFN)-γ/tumour necrosis factor (TNF)-α-stimulated HaCaT cells and in a mouse model of imiquimod (IMQ)-induced psoriasis. Read More

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Dorsomorphin attenuates Jagged1-induced mineralization in human dental pulp cells.

Int Endod J 2021 Aug 29. Epub 2021 Aug 29.

Dental Stem Cell Biology Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.

Aim: To investigate whether TGF-β/BMP signalling participates in Jagged1-induced osteogenic differentiation in human dental pulp cells (hDPs).

Methodology: Bioinformatic analysis of publicly available RNA sequencing data of Jagged1-treated hDPs was performed using NetworkAnalyst. The mRNA expression was validated using real-time polymerase chain reaction. Read More

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