851,520 results match your criteria stage to 5methods


Rice TSV2 encoding threonyl-tRNA synthetase is needed for early chloroplast development and seedling growth under cold stress.

G3 (Bethesda) 2021 Sep;11(9)

College of Life Sciences, Shanghai Normal University, Shanghai 200234, China.

Threonyl-tRNA synthetase (ThrRS), one of the aminoacyl-tRNA synthetases (AARSs), plays a crucial role in protein synthesis. However, the AARS functions on rice chloroplast development and growth were not fully appraised. In this study, a thermo-sensitive virescent mutant tsv2, which showed albino phenotype and lethal after the 4-leaf stage at 20°C but recovered to normal when the temperatures rose, was identified and characterized. Read More

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September 2021

Differences in temperature-sensitive expression of PcG-regulated genes among natural populations of Drosophila melanogaster.

G3 (Bethesda) 2021 Sep;11(9)

Applied Zoology, Faculty of Biology, Technische Universität Dresden, Dresden 01217, Germany.

Environmental temperature can affect chromatin-based gene regulation, in particular in ectotherms such as insects. Genes regulated by the Polycomb group (PcG) vary in their transcriptional output in response to changes in temperature. Expression of PcG-regulated genes typically increases with decreasing temperatures. Read More

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September 2021

Comparison of Outcomes between Percutaneous and Surgical Placement of Peritoneal Dialysis Catheters in Uremic Patients: A Meta-Analysis.

Blood Purif 2021 Sep 20:1-17. Epub 2021 Sep 20.

Division of Nephrology, Kidney Institute of CPLA, Changzheng Hospital, Second Military Medical University, Shanghai, China.

Background: The optimal technique for inserting peritoneal dialysis catheters in uremic patients remains debated. This meta-analysis aimed to summarize the current evidence evaluating the efficacy and safety of percutaneous insertion methods compared to surgical methods.

Method: A literature search was performed in the PubMed, EMBASE, Cochrane, and Web of Science databases. Read More

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September 2021

Biomimetic synthesis of a novel O2-regeneration nanosystem for enhanced starvation/chemo-therapy.

Nanotechnology 2021 Sep 20. Epub 2021 Sep 20.

Qufu Normal University, qufu, Qufu, Shandong, 273165, CHINA.

Glucose oxidase (GOX)-mediated starvation therapy that effectively cuts off energy supply holds great promise in cancer treatment. However, high glutathione (GSH) contents and anoxic conditions severely reduce therapy efficiency and cannot fully kill cancer cells. Herein, to resolve the above problem, this study constructed a biomimetic nanosystem based on nanreproo-MnO2 with porous craspedia globose-like structure and high specific surface area, and it was further modified with dopamine and folic acid to guarantee good biocompatibility and selectivity toward cancer cells. Read More

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September 2021

Formation of the octadecylphosphonic acid layer on the surface of Ti6Al4V ELI titanium alloy and analysis using Raman spectroscopy.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Sep 10;265:120368. Epub 2021 Sep 10.

Institute of Materials Research and Quantum Engineering, Poznan University of Technology, Poland.

Increasing life expectancy, a sedentary lifestyle and bone diseases all contribute to an increasing demand for endoprostheses. Currently, the service life of a knee prosthesis is 10-17 years on average, depending on the patient's weight and activity. In addition, the most common reasons for revision operations after implantation are prosthesis loosening and infections resulting from the lack of implant-bone connection. Read More

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September 2021

Estimating monthly PM concentrations from satellite remote sensing data, meteorological variables, and land use data using ensemble statistical modeling and a random forest approach.

Environ Pollut 2021 Sep 14;291:118159. Epub 2021 Sep 14.

Institute of Environmental and Occupational Health Sciences, School of Public Health, National Taiwan University, Taiwan. Electronic address:

Fine particulate matter (PM) is associated with various adverse health outcomes and poses serious concerns for public health. However, ground monitoring stations for PM measurements are mostly installed in population-dense or urban areas. Thus, satellite retrieved aerosol optical depth (AOD) data, which provide spatial and temporal surrogates of exposure, have become an important tool for PM estimates in a study area. Read More

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September 2021

Recurrent neural network from adder's perspective: Carry-lookahead RNN.

Neural Netw 2021 Sep 6;144:297-306. Epub 2021 Sep 6.

School of Computer Science and Technology, Hangzhou Dianzi University, China. Electronic address:

The recurrent network architecture is a widely used model in sequence modeling, but its serial dependency hinders the computation parallelization, which makes the operation inefficient. The same problem was encountered in serial adder at the early stage of digital electronics. In this paper, we discuss the similarities between recurrent neural network (RNN) and serial adder. Read More

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September 2021

MiR-200c promotes papillary thyroid cancer cell proliferation, migration, and invasion by downregulating PTEN.

Tissue Cell 2021 Sep 10;73:101647. Epub 2021 Sep 10.

Department of General Surgery, the First People's Hospital of Yancheng, Yancheng, Jiangsu Province, 224005, PR China. Electronic address:

MiR-200c has been reported in several types of human cancer. Nevertheless, the expression profile and biological functions of miR-200c remain uncovered papillary thyroid cancer (PTC). The expression level of miR-200c was evaluated in PTC tissues using RT-qPCR. Read More

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September 2021

Transverse position. Using Rotation to aid Normal birth - OUTcomes following manual rotation (The TURN-OUT Trial): A randomized controlled trial.

Am J Obstet Gynecol MFM 2021 Sep 17:100488. Epub 2021 Sep 17.

RPA Women & Babies, Royal Prince Alfred Hospital, Sydney, NSW, Australia; Sydney Institute for Women Children and their Families/Sydney Local Health District.

Background: Fetal occiput transverse position in the second stage of labor is associated with adverse maternal and perinatal outcomes. Prophylactic manual rotation in the second stage of labor is considered a safe and easy to perform procedure that has been used to prevent operative deliveries.

Objective: To determine the efficacy of prophylactic manual rotation in the management of occiput transverse position for preventing operative delivery. Read More

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September 2021

The Society for Vascular Surgery Objective Performance Goals for Critical Limb Ischemia are attainable in select patients with ischemic wounds managed with wound care alone.

Ann Vasc Surg 2021 Sep 17. Epub 2021 Sep 17.

Division of Vascular Surgery, Department of Surgery, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC. Electronic address:

Objectives: To set therapeutic benchmarks, in 2009 the Society for Vascular Surgery defined objective performance goals (OPG) for treatment of patients with chronic limb threatening ischemia (CLTI) with either open surgical bypass or endovascular intervention. The goal of these OPGs are to set standards of care from a revascularization standpoint and to provide performance benchmarks for 1 year patency rates for new endovascular therapies. While OPGs are useful in this regard, a critical decision point in the treatment of patients with CLTI is determining when revascularization is necessary. Read More

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September 2021

Clinical and Pathological Correlations in Chronic Venous Disease.

Ann Vasc Surg 2021 Sep 17. Epub 2021 Sep 17.

Department of Health Sciences, University of Catanzaro, 88100 Catanzaro, Italy.

Background: Chronic Venous Disease (CVD) has a high prevalence in the western world. Varicose veins (VVs) are the main signs of this disease that is characterized by important pathological vessel wall changes. The aim of this study is to correlate the main histopathological abnormalities with related clinical issues of CVD. Read More

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September 2021

Exposure of silver nanocolloids causes glycosylation disorders and embryonic deformities in medaka.

Toxicol Appl Pharmacol 2021 Sep 17:115714. Epub 2021 Sep 17.

Graduate School of Life Sciences, Toyo University, 1-1-1 Izumino, Itakura, Gunma 374-0193, Japan; Research Center for Life and Environmental Sciences, Toyo University, 1-1-1 Izumino, Itakura, Gunma 374-0193, Japan; Department of Life Sciences, Toyo University, 1-1-1 Izumino, Itakura, Gunma 374-0193, Japan. Electronic address:

Silver nanomaterials such as silver nanocolloids (SNC) contribute to environmental pollution and have adverse ecological effects on aquatic organisms. In particular, chemical exposure of fish during embryogenesis leads to deformities and puts the population at risk. Although glycans and glycosylation are known to be important for proper morphology in embryogenesis, little glycobiology-based research has examined morphological disorders caused by environmental pollutants. Read More

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September 2021

Establishment of transgenic zebrafish (Danio rerio) models expressing fluorescence proteins in the oocytes and somatic supporting cells.

Gen Comp Endocrinol 2021 Sep 17:113907. Epub 2021 Sep 17.

Centre of Reproduction, Development and Aging (CRDA), Faculty of Health Sciences, University of Macau, Taipa, Macau, China. Electronic address:

The interaction between gonadal somatic support cells and germ cells plays a crucial role in gonadal development. In fish, the process involves various local growth factors such as growth differentiation factor 9 (Gdf9) and gonadal soma-derived factor (Gsdf), which are both members of the transforming growth factor-β (TGF-β) superfamily. Gdf9, an oocyte-secreted factor, is a potent regulator of folliculogenesis in both mammals and fish. Read More

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September 2021

Regulation of inflammation and COX-2 gene expression in benzo (a) pyrene induced lung carcinogenesis in mice by all trans retinoic acid (ATRA).

Life Sci 2021 Sep 17:119967. Epub 2021 Sep 17.

Galaxy Health Care, Kolkata 700 156, West Bengal, India.

Aim: Inflammation provides favourable microenvironment for cancer development. An enhanced COX-2 gene expression is a key inflammatory mediator of cancers and the drug that inhibits it, helps to manage cancer effectively and increases survival rate. The objective is to analyse the inflammatory changes and COX-2 gene expression in benzo (a) pyrene induced mice and to evaluate the regulatory effect of all trans retinoic acid. Read More

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September 2021

The role of iron-based nanoparticles (Fe-NPs) on methanogenesis in anaerobic digestion (AD) performance.

Environ Res 2021 Sep 17:112043. Epub 2021 Sep 17.

Faculty of Civil Engineering Technology, Universiti Malaysia Pahang (UMP), Lebuhraya Tun Razak, Gambang, Kuantan, Pahang, 26300, Malaysia. Electronic address:

Several strategies have been proposed to improve the performance of the anaerobic digestion (AD) process. Among them, the use of various nanoparticles (NPs) (e.g. Read More

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September 2021

Extended therapy with letrozole as adjuvant treatment of postmenopausal patients with early-stage breast cancer: a multicentre, open-label, randomised, phase 3 trial.

Lancet Oncol 2021 Sep 15. Epub 2021 Sep 15.

Epidemiology Unit, IRCCS Ospedale Policlinico San Martino, Genoa, Italy.

Background: The benefit of extending aromatase inhibitor therapy beyond 5 years in the context of previous aromatase inhibitors remains controversial. We aimed to compare extended therapy with letrozole for 5 years versus the standard duration of 2-3 years of letrozole in postmenopausal patients with breast cancer who have already received 2-3 years of tamoxifen.

Methods: This multicentre, open-label, randomised, phase 3 trial was done at 69 hospitals in Italy. Read More

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September 2021

Vitamin D in systemic lupus erythematosus with and without lupus nephritis.

Rev Med Inst Mex Seguro Soc 2020 ;58(4):394-399

Laboratorios Clínicos de Puebla, Sucursal Anzures, Dirección de Proyectos Especiales. Puebla, Puebla, México.

IntroducciÓn: Las concentraciones séricas subóptimas de vitamina D se presentan en múltiples enfermedades crónicas, como las enfermedades autoinmunitarias. Los objetivos del estudio fueron: 1) comparar las concentraciones séricas de 25-hidroxivitamina D (25OHD3) en pacientes con lupus eritematoso sistémico (LES) con y sin nefritis lúpica (NL), y 2) evaluar la asociación de las concentraciones séricas de 25OHD3 con la actividad de la enfermedad.

Material Y MÉtodos: Estudio comparativo, transversal, que incluyó 48 mujeres con LES, con y sin NL. Read More

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January 2020

On normalizing of urinary KIM-1 level to urine creatinine in patients with renal cell cancer.

Klin Lab Diagn 2021 Sep;66(9):517-524

Federal State Budgetary Institution "National Medical Research Radiological Centre", Ministry of Health of the Russian Federation.

KIM-1 (kidney injury molecule 1), a marker of acute kidney injury, is produced by epithelial cells of renal proximal tubules. Elevated KIM-1 levels in urine and plasma are associated with renal cell carcinoma (RCC). The aim of this study was to compare the significance of non-normalized uKIM-1 values and those normalized to urine creatinine, as urinary biomarkers in RCC. Read More

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September 2021

Label-free quantitative proteomic and phosphoproteomic analyses of renal biopsy tissues in membranous nephropathy.

Proteomics Clin Appl 2021 Sep 20:e2000069. Epub 2021 Sep 20.

Clinical Medical Research Center, The Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, 518020, China.

Purpose: Membranous nephropathy (MN) is a common cause of nephrotic syndrome in adults. However, the underlying mechanisms of its occurrence and development are not completely clear. Thus, it is essential to explore the mechanisms. Read More

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September 2021

Increasing the spatial and temporal impact of ecological research: A roadmap for integrating a novel terrestrial process into an Earth system model.

Glob Chang Biol 2021 Sep 20. Epub 2021 Sep 20.

Department of Natural Resources and the Environment, University of New Hampshire, Durham, 03824.

Terrestrial ecosystems regulate Earth's climate through water, energy, and biogeochemical transformations. Despite a key role in regulating the Earth system, terrestrial ecology has historically been underrepresented in the Earth system models (ESMs) that are used to understand and project global environmental change. Ecology and Earth system modeling must be integrated for scientists to fully comprehend the role of ecological systems in driving and responding to global change. Read More

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September 2021

Context dependent fitness costs of reproduction despite stable body mass costs in an Arctic herbivore.

J Anim Ecol 2021 Sep 20. Epub 2021 Sep 20.

Department for Arctic Ecology, Norwegian Institute for Nature Research, Fram Centre, NO-9296, Tromsø, Norway.

The cost of reproduction on demographic rates is often assumed to operate through changing body condition. Several studies have found that reproduction depresses body mass more if the current conditions are severe, such as high population densities or adverse weather, than under benign environmental conditions. However, few studies have investigated the association between the fitness and body mass costs of reproduction. Read More

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September 2021

Engineering Sequence and Selectivity of Late-Stage C-H Oxidation in the MycG Iterative Cytochrome P450.

J Ind Microbiol Biotechnol 2021 Sep 20. Epub 2021 Sep 20.

Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi, Chiba, Japan.

MycG is a multifunctional P450 monooxygenase that catalyzes sequential hydroxylation and epoxidation or a single epoxidation in mycinamicin biosynthesis. In the mycinamicin-producing strain Micromonospora griseorubida A11725, very low level accumulation of mycinamicin V generated by the initial C-14 allylic hydroxylation of MycG is observed due to its subsequent epoxidation to generate mycinamicin II, the terminal metabolite in this pathway. Herein, we investigated whether MycG can be engineered for production of the mycinamicin II intermediate as the predominant metabolite. Read More

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September 2021

Optimal Thyroid Hormone Replacement.

Endocr Rev 2021 Sep 20. Epub 2021 Sep 20.

Georgetown University, Division of Endocrinology, Washington, DC, USA.

Hypothyroidism is a common endocrinopathy and levothyroxine is frequently prescribed. Despite the basic tenets of initiating and adjusting levothyroxine being agreed upon, there are many nuances and complexities to consistently maintaining euthyroidism. Understanding the impact of patient weight and residual thyroid function on initial levothyroxine dosage and consideration of age, co-morbidities, TSH goal, life stage, and quality of life as levothyroxine is adjusted can be challenging and continually evolving. Read More

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September 2021

HGAL inhibits lymphoma dissemination by interacting with multiple Cytoskeletal proteins.

Blood Adv 2021 Sep 20. Epub 2021 Sep 20.

University of Miami, Miami, Florida, United States.

Human Germinal Center Associated Lymphoma (HGAL) is an adaptor protein specifically expressed in germinal center lymphocytes. High expression of HGAL is a predictor of prolonged survival of Diffuse Large B-Cell (DLBCL) and classical Hodgkin lymphomas. Furthermore, HGAL expression is associated with early stage DLBCL, thus potentially limiting lymphoma dissemination. Read More

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September 2021

Potassium fertilization improves growth, yield and seed quality of sunflower (Helianthus annuus L.) under drought stress at different growth stages.

PLoS One 2021 20;16(9):e0256075. Epub 2021 Sep 20.

Department of Biology, College of Sciences, Taif University, Taif, Saudi Arabia.

Water scarcity is a major concern for sunflower production in the semi-arid and arid regions of the world. Potassium (K) application has been found effective to alleviate the influence of drought stress; however, the impact of drought stress on seed quality of sunflower has not been reported frequently. Therefore, a field experiment was performed to determine the optimum K requirement for mitigating the adverse effects of water stress and improving growth and seed quality of spring-planted sunflower. Read More

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September 2021

Effects of aerobic exercise and resistance exercise on physical indexes and cardiovascular risk factors in obese and overweight school-age children: A systematic review and meta-analysis.

PLoS One 2021 20;16(9):e0257150. Epub 2021 Sep 20.

Department of Rehabilitation Sciences, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.

Background: Obesity is a serious social and public health problem in the world, especially in children and adolescents. For school-age children with obesity, this stage is in the transition from childhood to adolescence, and both physical, psychological, and external environments will be full of challenges. Studies have showed that school-age children are the largest proportion of people who continue to be obese in adulthood. Read More

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September 2021

Uncertainty of methane emissions coming from the physical volume of plant biomass inside the closed chamber was negligible during cropping period.

PLoS One 2021 20;16(9):e0256796. Epub 2021 Sep 20.

Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration (RDA), Wanju, Korea.

In rice paddy, the closed chamber method is broadly used to estimate methane (CH4) emission rate. Since rice plants can significantly affect CH4 production, oxidation and emission, rice plantation inside the chamber is standardized in IPCC guidelines. Methane emission rate is calculated using the increased concentration inside the headspace. Read More

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September 2021

Trypanosoma cruzi infection follow-up in a sylvatic vector of Chagas disease: Comparing early and late stage nymphs.

PLoS Negl Trop Dis 2021 Sep 20;15(9):e0009729. Epub 2021 Sep 20.

Programa Biología Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile, Santiago, Chile.

Chagas disease is caused by Trypanosoma cruzi and transmitted by the triatomine Mepraia spinolai in the southwest of South America. Here, we examined the T. cruzi-infection dynamics of field-caught M. Read More

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September 2021

Sex differences in psychotropic and analgesic drug use before and after initiating treatment with acetylcholinesterase inhibitors.

PLoS One 2021 20;16(9):e0243804. Epub 2021 Sep 20.

Norwegian Institute of Public Health, Oslo, Norway.

Background/aims: The aim was to explore the impact of sex on prevalence, patterns and trends in the prescription of psychotropics and analgesics in users of acetylcholinesterase inhibitors (AChEIs), before and after AChEI initiation, compared to the general population.

Methods: A prospective study applying data from the Norwegian Prescription Database (NorPD) in the period 2004-2016. Prescription of antidepressants, antipsychotics, analgesics including opioids, benzodiazepines and z-hypnotics in persistent AChEI users was studied in a follow-up period from four years before to two years after AChEI initiation in men and women of four age groups: 37-64, 65-72, 73-80 and 81-88 years. Read More

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September 2021

The core seed mycobiome of var. across provenances of the Pacific Northwest, USA.

Mycologia 2021 Sep 20:1-12. Epub 2021 Sep 20.

Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331.

Fungal symbionts occur in all plant tissues, and many aid their host plants with critical functions, including nutrient acquisition, defense against pathogens, and tolerance of abiotic stress. "Core" taxa in the plant mycobiome, defined as fungi present across individuals, populations, or time, may be particularly crucial to plant survival during the challenging seedling stage. However, studies on core seed fungi are limited to individual sampling sites, raising the question of whether core taxa exist across large geographic scales. Read More

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September 2021