22,159 results match your criteria stably expressed

11βHSD2 Efficacy in Preventing Transcriptional Activation of the Mineralocorticoid Receptor by Corticosterone.

J Endocr Soc 2021 Nov 3;5(11):bvab146. Epub 2021 Sep 3.

Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi 39216, USA.

Affinity of the mineralocorticoid receptor (MR) is similar for aldosterone and the glucocorticoids (GC) cortisol and corticosterone, which circulate at concentrations far exceeding those of aldosterone. 11β-hydroxysteroid dehydrogenase type 2 (11βHSD2) inactivation of GC within the immediate vicinity of the MR is credited with prereceptor specificity for aldosterone in cells coexpressing MR and 11βHSD2. 11βHSD2 efficacy is also critical to other recently described 11βHSD2 substrates. Read More

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November 2021

Enhances Accumulation of Above-Ground Biomass and Recombinant Proteins in Transgenic Plants.

Front Plant Sci 2021 10;12:712438. Epub 2021 Sep 10.

Institute for Biosafety in Plant Biotechnology, Julius Kühn-Institut (JKI) - Federal Research Centre for Cultivated Plants, Quedlinburg, Germany.

Transient expression in holds great potential for recombinant protein manufacturing due to its advantages in terms of speed and yield compared to stably transformed plants. To continue improving the quantity of recombinant proteins the plant host will need to be modified at both plant and cellular levels. In attempt to increase leaf mass fraction, we transformed with the gene, a positive regulator of the cell cycle. Read More

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September 2021

HLA-G1 Expression in GGTA1KO Pigs Suppresses Human and Monkey Anti-Pig T, B and NK Cell Responses.

Front Immunol 2021 9;12:730545. Epub 2021 Sep 9.

Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, MN, United States.

The human leukocyte antigen G1 (HLA-G1), a non-classical class I major histocompatibility complex (MHC-I) protein, is a potent immunomodulatory molecule at the maternal/fetal interface and other environments to regulate the cellular immune response. We created GGTA1/HLAG1 pigs to explore their use as organ and cell donors that may extend xenograft survival and function in both preclinical nonhuman primate (NHP) models and future clinical trials. In the present study, HLA-G1 was expressed from the porcine ROSA26 locus by homology directed repair (HDR) mediated knock-in (KI) with simultaneous deletion of α-1-3-galactotransferase gene (GGTA1; GTKO) using the clustered regularly interspersed palindromic repeats (CRISPR)/CRISPR associated protein 9 (Cas9) (CRISPR/Cas9) gene-editing system. Read More

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September 2021

A potential diagnostic marker for osteosarcoma: hsa_circ_0005721.

J BUON 2021 Jul-Aug;26(4):1582-1588

Department of Traumatic Orthopedics, Yantaishan Hospital of Yantai, Yantai, China.

Purpose: To detect the expression level of hsa_circ_0005721 in osteosarcoma specimen and plasma of osteosarcoma patients, and to analyze the clinical significance of hsa_circ_0005721 as a diagnostic marker for osteosarcoma.

Methods: Expression levels of hsa_circ_0005721 in osteosarcoma specimen and osteosarcoma cell lines were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The relationship between hsa_circ_0005721 expression difference and overall survival in osteosarcoma was analyzed by Kaplan-Meier method. Read More

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September 2021

The lncRNA Promotes Hepatocellular Carcinoma Cell Cycle Progression by Regulating the HuR/CDK4 Axis.

DNA Cell Biol 2021 Sep 24. Epub 2021 Sep 24.

Lanzhou University Second Hospital, Lanzhou, Gansu, China.

Long noncoding RNAs (lncRNAs) represent promising therapeutic targets associated with hepatocellular carcinoma (HCC). lncRNA VPS9D1 antisense RNA 1 () regulates colon and prostate cancer, but its relevance in HCC remains to be clarified. Using microarray data from the NCBI Gene Expression Omnibus (GEO) database (GSE65485) and The Cancer Genome Atlas (TCGA) database, expression in HCC and normal liver tissue sample HCC were compared. Read More

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September 2021

Glypican 4 Regulates Aβ Internalization in Neural Stem Cells Partly Low-Density Lipoprotein Receptor-Related Protein 1.

Front Cell Neurosci 2021 6;15:732429. Epub 2021 Sep 6.

Institute of Neurobiology, Department of Neurobiology, Xi'an Jiaotong University Health Science Center, Xi'an, China.

Neural stem cell (NSC) damage has been reported in patients with Alzheimer's disease. Intracellular Aβ plays a vital role in NSC damage. Heparan sulfate proteoglycans are potent mediators of Aβ enrichment in the brain. Read More

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September 2021

The Efficacy of Schwann-Like Differentiated Muscle-Derived Stem Cells in Treating Rodent Upper Extremity Peripheral Nerve Injury.

Plast Reconstr Surg 2021 Oct;148(4):787-798

From the Department of Plastic and Reconstructive Surgery and the Translational Tissue Engineering Center, The Johns Hopkins University School of Medicine; the Department of Materials Science and Engineering, Whiting School of Engineering, the Institute for NanoBioTechnology, and the Department of Neuroscience, The Johns Hopkins University; and the Division of Plastic and Reconstructive Surgery, University Hospitals Cleveland Medical Center.

Background: There is a pressing need to identify alternative mesenchymal stem cell sources for Schwann cell cellular replacement therapy, to improve peripheral nerve regeneration. This study assessed the efficacy of Schwann cell-like cells (induced muscle-derived stem cells) differentiated from muscle-derived stem cells (MDSCs) in augmenting nerve regeneration and improving muscle function after nerve trauma.

Methods: The Schwann cell-like nature of induced MDSCs was characterized in vitro using immunofluorescence, flow cytometry, microarray, and reverse-transcription polymerase chain reaction. Read More

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October 2021

New drought-adaptive loci underlying candidate genes on wheat chromosome 4B with improved photosynthesis and yield responses.

Physiol Plant 2021 Sep 22. Epub 2021 Sep 22.

Institute of Crop Science and Resource Conservation (INRES)-Plant Breeding and Biotechnology, University of Bonn, Bonn, Germany.

Flag leaf serves as an essential source of assimilates during grain filling, thereby contributing to grain yield up to 48%. Thus, high-throughput phenotyping of flag leaves is crucial to determine their physiological and genetic basis of yield formation and drought adaptation. Here, we utilized 200 wheat cultivars to identify drought-adaptive loci underlying candidate genes associated with flag leaf biomass and photosynthesis-related traits using a genome-wide association study (GWAS). Read More

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September 2021

Spontaneous calcium responses of SF2 rat dental epithelial cells stably expressing the calcium sensor G-GECO.

Biomed Res 2021 ;42(5):193-201

Division of Pharmacology, Department of Oral Biology, School of Dentistry, Health Sciences University of Hokkaido.

Genetically-encoded calcium indicators such as G-GECO are useful for studying Ca responses during long-term processes. In this study, we employed a lentiviral vector and established a rat dental epithelial cell line that stably expressed G-GECO (SF2-G-GECO). Ca imaging analysis under cell culture conditions revealed that SF2-G-GECO cells exhibited spontaneous Ca responses, which could be classified into the following three major patterns depending on the cell density: localized Ca responses at cell protrusions at a low density, a cell-wide spread of Ca responses at a medium density, and Ca responses in clusters of 3-20 cells at a high density. Read More

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January 2021

The Role of the Wnt Pathway in VEGF/Anti-VEGF-Dependent Control of the Endothelial Cell Barrier.

Invest Ophthalmol Vis Sci 2021 Sep;62(12):17

University of Illinois at Chicago, Department of Ophthalmology & Visual Sciences, Chicago, IL, United States.

Purpose: Investigate the contribution of the Wnt pathway to vascular endothelial growth factor (VEGF)/anti-VEGF-mediated control of endothelial cell permeability.

Methods: High glucose-treated primary human retinal endothelial cells (HRECs) were exposed to either VEGF, or VEGF and then anti-VEGF. Changes in gene expression were assayed by RNAseq and qRT-PCR. Read More

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September 2021

TFEB promotes BCL-2 expression by upregulating its promoter activity in the t(6;11) translocation renal cell carcinomas.

Am J Transl Res 2021 15;13(8):8804-8818. Epub 2021 Aug 15.

Institute of Clinical Virology, Anhui Medical University Hefei 230032, Anhui, China.

t(6;11) translocation renal cell carcinoma (RCC) is classified as a subset of the MiT family translocation RCCs and characterized by harboring the Alpha-TFEB fusion gene. However, the development mechanism of this tumor and its effective treatment have not been fully identified yet. The purpose of this study was to explore the relationship between TFEB and BCL-2 in Alpha-TFEB stably transfected cell lines and in t(6;11) RCC tumor tissue. Read More

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Identification and validation of reference genes for RT-qPCR analysis in fetal rat pancreas.

Reprod Toxicol 2021 Sep 16;105:211-220. Epub 2021 Sep 16.

Department of Pharmacology, Wuhan University School of Basic Medical Sciences, Wuhan, 430071, Hubei Province, People's Republic of China; Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan, 430071, Hubei Province, People's Republic of China. Electronic address:

The choice of reference gene is crucial for quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) assay. To screen and determine the suitable reference genes in fetal rat pancreas, we selected eight candidate reference genes (Gapdh, Actb, Rn18 s, B2m, Rpl13a, Tbp, Ywhaz and Ubc), and evaluated the constancy of gene expression from fetal rat pancreases in non-pathological situation and prenatal dexamethasone exposure (PDE) model, using four algorithms: GeNorm, NormFinder, Bestkeeper and Comparative ΔCt method. In addition, the alteration of mRNA levels of pancreatic insulin was compared between control and PDE groups to validate the reliability of selected reference genes for data normalization of RT-qPCR. Read More

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September 2021

Generation of stable cell lines using readthrough expression from lentiviral integration.

Biotechnol Lett 2021 Sep 17. Epub 2021 Sep 17.

Institute of Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON, M5S 3G9, Canada.

Lentiviral infection is often used to integrate genetic material into cells to stably express transgenes of interest. Depending on the location of integration into the host genome, readthrough expression of the lentiviral cargo can occur via an upstream endogenous promoter, which is typically an unwanted phenomenon because it can result in dysfunctional expression. The purpose of this study was to demonstrate that readthrough expression can be a wanted phenomenon for expressing functional proteins while at the same time reducing the size of the lentiviral transfer plasmid. Read More

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September 2021

Creating a novel petal regeneration system for function identification of colour gene of grape hyacinth.

Plant Methods 2021 Sep 16;17(1):94. Epub 2021 Sep 16.

College of Landscape Architecture and Arts, Northwest A & F University, Yangling, 712100, Shaanxi, People's Republic of China.

Background: Grape hyacinth (Muscari spp.) is one of the most important ornamental bulbous plants. However, its lengthy juvenile period and time-consuming transformation approaches under the available protocols impedes the functional characterisation of its genes in flower tissues. Read More

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September 2021

Exosome encapsulated ncRNAs in the development of HCC: potential circulatory biomarkers and clinical therapeutic targets.

Am J Cancer Res 2021 15;11(8):3794-3812. Epub 2021 Aug 15.

Department of Oncology, The First Affiliated Hospital of Nanjing Medical University Nanjing 210029, Jiangsu, China.

Hepatocellular carcinoma (HCC) is the sixth most deadly malignant cancer in the world and has the third highest mortality rate among cancer-related deaths worldwide. Its poor prognosis can be attributed to late diagnosis, high risk of recurrence and drug resistance. Therefore, finding a new biomarker to help us in the early diagnosis, and exploring the molecular mechanisms involved in recurrence and drug resistance is a reasonable research direction for clinical treatment of HCC. Read More

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Development of a novel reporter gene assay to evaluate antibody-dependent cellular phagocytosis for anti-CD20 therapeutic antibodies.

Int Immunopharmacol 2021 Sep 11;100:108112. Epub 2021 Sep 11.

Division of Monoclonal Antibody Products, National Institu-tes for Food and Drug Control, Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, Beijing 102629, China.

More than 100 monoclonal antibodies (mAbs) have been approved by FDA. The mechanism of action (MoA) involves in neutralization of a specific target via the Fab region and Fc effector functions through Fc region, while the latter include complement-dependent cytotoxicity (CDC), antibody-dependent cell-mediated cytotoxicity (ADCC) and antibody-dependent cellular phagocytosis (ADCP). ADCP has been recognized one of the most important MoAs, especially for anti-cancer mAbs in recent years. Read More

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September 2021

Down-regulation of lncRNA MEG3 promotes chronic low dose cadmium exposure-induced cell transformation and cancer stem cell-like property.

Toxicol Appl Pharmacol 2021 Sep 11;430:115724. Epub 2021 Sep 11.

Division of Cancer Biology, Department of Medicine, MetroHealth Medical Center, Case Western University School of Medicine, Cleveland, OH 44109, United States of America. Electronic address:

Cadmium (Cd) is a toxic heavy metal and one of carcinogens that cause lung cancer. However, the exact mechanism of Cd carcinogenesis remains unclear. To investigate the mechanism of Cd carcinogenesis, we exposed human bronchial epithelial cells (BEAS-2B) to a low dose of Cd (2. Read More

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September 2021

Comparison of transcriptional initiation by RNA polymerase II across eukaryotic species.

Elife 2021 09 13;10. Epub 2021 Sep 13.

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, United States.

The preinitiation complex (PIC) for transcriptional initiation by RNA polymerase (Pol) II is composed of general transcription factors that are highly conserved. However, analysis of ChIP-seq datasets reveals kinetic and compositional differences in the transcriptional initiation process among eukaryotic species. In yeast, Mediator associates strongly with activator proteins bound to enhancers, but it transiently associates with promoters in a form that lacks the kinase module. Read More

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September 2021

Oxidative Stress Resistance 1 Gene Therapy Retards Neurodegeneration in the rd1 Mutant Mouse Model of Retinopathy.

Invest Ophthalmol Vis Sci 2021 09;62(12)

NeuroNexus Institute, University of Massachusetts Medical School, Worcester, Massachusetts, United States.

Purpose: Oxidative stress is a major factor underlying many neurodegenerative diseases. However, antioxidant therapy has had mixed results, possibly because of its indiscriminate activity. The purpose of our study was to determine if the human OXR1 (hOXR1) antioxidant regulatory gene could protect neurons from oxidative stress and delay photoreceptor cell death. Read More

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September 2021

Pseudomonas aeruginosa adapts to octenidine via a combination of efflux and membrane remodelling.

Commun Biol 2021 09 9;4(1):1058. Epub 2021 Sep 9.

Technology Development Group, National Infection Service, PHE Porton, Salisbury, UK.

Pseudomonas aeruginosa is an opportunistic pathogen capable of stably adapting to the antiseptic octenidine by an unknown mechanism. Here we characterise this adaptation, both in the laboratory and a simulated clinical setting, and identify a novel antiseptic resistance mechanism. In both settings, 2 to 4-fold increase in octenidine tolerance was associated with stable mutations and a specific 12 base pair deletion in a putative Tet-repressor family gene (smvR), associated with a constitutive increase in expression of the Major Facilitator Superfamily (MFS) efflux pump SmvA. Read More

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September 2021

Properties of Transport Mediated by the Human Organic Cation Transporter 2 Studied in a Polarized Three-Dimensional Epithelial Cell Culture Model.

Int J Mol Sci 2021 Sep 6;22(17). Epub 2021 Sep 6.

Medicine Clinic D, Experimental Nephrology, University Hospital of Münster, 48149 Münster, Germany.

The renal secretory clearance for organic cations (neurotransmitters, metabolism products and drugs) is mediated by transporters specifically expressed in the basolateral and apical plasma membrane domains of proximal tubule cells. Here, human organic cation transporter 2 (hOCT2) is the main transporter for organic cations in the basolateral membrane domain. In this study, we stably expressed hOCT2 in Madin-Darby Canine Kidney (MDCK) cells and cultivated these cells in the presence of an extracellular matrix to obtain three-dimensional (3D) structures (cysts). Read More

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September 2021

miRNAs as Regulators of the Early Local Response to Burn Injuries.

Int J Mol Sci 2021 Aug 26;22(17). Epub 2021 Aug 26.

Department of Internal Medicine, Division of Endocrinology and Diabetology, Medical University of Graz, 8036 Graz, Austria.

In burn injuries, risk factors and limitations to treatment success are difficult to assess clinically. However, local cellular responses are characterized by specific gene-expression patterns. MicroRNAs (miRNAs) are single-stranded, non-coding RNAs that regulate mRNA expression on a posttranscriptional level. Read More

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Improved bacterial leaf blight disease resistance in the major elite Vietnamese rice cultivar TBR225 via editing of the OsSWEET14 promoter.

PLoS One 2021 9;16(9):e0255470. Epub 2021 Sep 9.

Department of Molecular Pathology, Institute of Agricultural Genetics, Vietnam Academy of Agricultural Sciences, Hanoi, Vietnam.

TBR225 is one of the most popular commercial rice varieties in Northern Vietnam. However, this variety is highly susceptible to bacterial leaf blight (BLB), a disease caused by Xanthomonas oryzae pv. oryzae (Xoo) which can lead to important yield losses. Read More

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September 2021

Genetically Encoded Protein Thermometer Enables Precise Electrothermal Control of Transgene Expression.

Adv Sci (Weinh) 2021 Sep 8:e2101813. Epub 2021 Sep 8.

ETH Zürich, Department of Biosystems Science and Engineering, Mattenstrasse 26, Basel, 4058, Switzerland.

Body temperature is maintained at around 37 °C in humans, but may rise to 40 °C or more during high-grade fever, which occurs in most adults who are seriously ill. However, endogenous temperature sensors, such as ion channels and heat-shock promoters, are fully activated only at noxious temperatures above this range, making them unsuitable for medical applications. Here, a genetically encoded protein thermometer (human enhanced gene activation thermometer; HEAT) is designed that can trigger transgene expression in the range of 37-40 °C by linking a mutant coiled-coil temperature-responsive protein sensor to a synthetic transcription factor. Read More

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September 2021

Two newly introduced endosymbionts induce cell host differences in competitiveness and metabolic responses.

Appl Environ Microbiol 2021 Sep 8:AEM0147921. Epub 2021 Sep 8.

Department of Entomology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.

endosymbionts can induce multiple reproductive manipulations in their hosts, with cytoplasmic incompatibility (CI) being one of the most common manipulations. The important agricultural pests, white-backed planthopper () and brown planthopper (), are usually infected with CI-inducing Fur and non-CI-inducing Lug, respectively. The biological effects of these infections when present in a host cell are unknown. Read More

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September 2021

Pathogenic T Cells in Celiac Disease Change Phenotype on Gluten Challenge: Implications for T-Cell-Directed Therapies.

Adv Sci (Weinh) 2021 Sep 8:e2102778. Epub 2021 Sep 8.

KG Jebsen Coeliac Disease Research Centre, University of Oslo, Oslo, 0372, Norway.

Gluten-specific CD4 T cells being drivers of celiac disease (CeD) are obvious targets for immunotherapy. Little is known about how cell markers harnessed for T-cell-directed therapy can change with time and upon activation in CeD and other autoimmune conditions. In-depth characterization of gluten-specific CD4 T cells and CeD-associated (CD38 and CD103 ) CD8 and γδ T cells in blood of treated CeD patients undergoing a 3 day gluten challenge is reported. Read More

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September 2021

Directed evolution of AAV accounting for long-term and enhanced transduction of cardiovascular endothelial cells .

Mol Ther Methods Clin Dev 2021 Sep 4;22:148-161. Epub 2021 Jun 4.

Department of Cardiology and Laboratory of Gene Therapy for Heart Diseases, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, Sichuan, China.

Cardiac endothelial cells (ECs) are important targets for cardiovascular gene therapy. However, the approach of stably transducing ECs using different vectors, including adeno-associated virus (AAV), remains unexamined. Regarding this unmet need, two AAV libraries from DNA shuffling and random peptide display were simultaneously screened in a transgenic mouse model. Read More

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September 2021

Production of therapeutic iduronate-2-sulfatase enzyme with a novel single-stranded RNA virus vector.

Genes Cells 2021 Sep 4. Epub 2021 Sep 4.

Department of Clinical Laboratory Medicine, National Center for Child Health and Development, Tokyo, Japan.

The Sendai virus vector has received a lot of attention due to its broad tropism for mammalian cells. As a result of efforts for genetic studies based on a mutant virus, we can now express more than 10 genes of up to 13.5 kilo nucleotides in a single vector with high protein expression efficiency. Read More

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September 2021

Molecular cloning and structural analysis of key enzymes in Tetrastigma hemsleyanum for resveratrol biosynthesis.

Int J Biol Macromol 2021 Sep 1;190:19-32. Epub 2021 Sep 1.

Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, PR China.

Resveratrol (RES), a plant antitoxin, has antioxidant, anti-inflammatory, anti-cancer and cardiovascular protection effects. It has been reported that RES can be stably detected in a Chinese herbal medicinal plant Tetrastigma hemsleyanum. At present, the research of T. Read More

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September 2021

LncRNA-H19 gene plays a significant role in regulating glioma cell function.

Mol Genet Genomic Med 2021 Sep 3:e1480. Epub 2021 Sep 3.

Department of Oncology, Heping Hospital Affiliated to Changzhi Medical College, Changzhi, China.

Background: Glioma is an aggressive adult primary cancer, and is characterized by low cure rate, poor prognosis, and high recurrence. The present study aimed to investigate the effect of lncRNA-H19 gene silencing on glioma cell function.

Methods: lncRNA-H19 interference vector (LV3-si-H19) and negative control vector (LV3-NC) were stably transfected into U251 and U87-MG cells, respectively. Read More

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September 2021