5,321 results match your criteria stabilization translation

AIRBP: Accurate identification of RNA-binding proteins using machine learning techniques.

Artif Intell Med 2021 03 13;113:102034. Epub 2021 Feb 13.

Department of Computer Science, University of New Orleans, New Orleans, LA, USA. Electronic address:

Identification of RNA-binding proteins (RBPs) that bind to ribonucleic acid molecules is an important problem in Computational Biology and Bioinformatics. It becomes indispensable to identify RBPs as they play crucial roles in post-transcriptional control of RNAs and RNA metabolism as well as have diverse roles in various biological processes such as splicing, mRNA stabilization, mRNA localization, and translation, RNA synthesis, folding-unfolding, modification, processing, and degradation. The existing experimental techniques for identifying RBPs are time-consuming and expensive. Read More

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A standardized approach for exact CT-based three-dimensional position analysis in the distal tibiofibular joint.

BMC Med Imaging 2021 03 6;21(1):41. Epub 2021 Mar 6.

Department of Orthopaedics, Trauma and Plastic Surgery, University Hospital Leipzig, Leipzig, Germany.

Background: Assessment of tibiofibular reduction presents an intra- and postoperative challenge. Numerous two-dimensional measurement methods have been described, most of them highly dependent on leg orientation and rater. Aim of the present work was to develop a standardized and orientation-independent 3D based method for the assessment of syndesmotic joint position. Read More

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How Is the Fidelity of Proteins Ensured in Terms of Both Quality and Quantity at the Endoplasmic Reticulum? Mechanistic Insights into E3 Ubiquitin Ligases.

Int J Mol Sci 2021 Feb 19;22(4). Epub 2021 Feb 19.

Department of Biochemistry, College of Medicine, Chungnam National University, Daejeon 35015, Korea.

The endoplasmic reticulum (ER) is an interconnected organelle that plays fundamental roles in the biosynthesis, folding, stabilization, maturation, and trafficking of secretory and transmembrane proteins. It is the largest organelle and critically modulates nearly all aspects of life. Therefore, in the endoplasmic reticulum, an enormous investment of resources, including chaperones and protein folding facilitators, is dedicated to adequate protein maturation and delivery to final destinations. Read More

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February 2021

STAU2 protein level is controlled by caspases and the CHK1 pathway and regulates cell cycle progression in the non-transformed hTERT-RPE1 cells.

BMC Mol Cell Biol 2021 Mar 4;22(1):16. Epub 2021 Mar 4.

Département de Biochimie et Médecine Moléculaire, Faculté de médecine, Université de Montréal, 2900 Édouard Montpetit, Montréal, QC, H3T 1J4, Canada.

Background: Staufen2 (STAU2) is an RNA binding protein involved in the posttranscriptional regulation of gene expression. In neurons, STAU2 is required to maintain the balance between differentiation and proliferation of neural stem cells through asymmetric cell division. However, the importance of controlling STAU2 expression for cell cycle progression is not clear in non-neuronal dividing cells. Read More

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VPS34 K29/K48 branched ubiquitination governed by UBE3C and TRABID regulates autophagy, proteostasis and liver metabolism.

Nat Commun 2021 02 26;12(1):1322. Epub 2021 Feb 26.

Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.

The ubiquitin-proteasome system (UPS) and autophagy are two major quality control processes whose impairment is linked to a wide variety of diseases. The coordination between UPS and autophagy remains incompletely understood. Here, we show that ubiquitin ligase UBE3C and deubiquitinating enzyme TRABID reciprocally regulate K29/K48-branched ubiquitination of VPS34. Read More

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February 2021

Functioning of PPR Proteins in Organelle RNA Metabolism and Chloroplast Biogenesis.

Front Plant Sci 2021 9;12:627501. Epub 2021 Feb 9.

Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing, China.

The pentatricopeptide repeat (PPR) proteins constitute one of the largest nuclear-encoded protein families in higher plants, with over 400 members in most sequenced plant species. The molecular functions of these proteins and their physiological roles during plant growth and development have been widely studied. Generally, there is mounting evidence that PPR proteins are involved in the post-transcriptional regulation of chloroplast and/or mitochondrial genes, including RNA maturation, editing, intron splicing, transcripts' stabilization, and translation initiation. Read More

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February 2021

Femorotibial joint kinematics in nine dogs treated with lateral suture stabilization for complete cranial cruciate ligament rupture.

J Am Vet Med Assoc 2021 Mar;258(5):493-501

Objective: To quantify 3-D femorotibial joint kinematics during ambulation in dogs with cranial cruciate ligament (CCL) rupture treated with lateral fabellotibial suture stabilization (LFTS).

Animals: 9 adult dogs (body weight, 15 to 35 kg [33 to 77 lb]) with unilateral complete CCL rupture.

Procedures: Digital 3-D bone models of the femur and fabellae and tibia and fibula were created from CT scans. Read More

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Stabilization of C/EBPβ through direct interaction with STAT3 in H-Ras transformed human mammary epithelial cells.

Biochem Biophys Res Commun 2021 03 11;546:130-137. Epub 2021 Feb 11.

Tumor Microenvironment Global Core Research Center, College of Pharmacy, Seoul National University, Seoul, 08826, South Korea; Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, 08826, South Korea; Cancer Research Institute, Seoul National University, Seoul, 03080, South Korea. Electronic address:

Signal transducer and activator of transcription 3 (STAT3) plays important roles in cancer-associated inflammation by controlling expression of proinflammatory cytokines and chemokines. Recent studies suggest that C/EBPβ (CCAAT-enhancer binding protein beta) and STAT3 synergistically stimulate cancer cell proliferation and epithelial-mesenchymal transition. C/EBPβ is a leucine-zipper transcription factor that regulates expression of a variety of inflammatory cytokines or chemokines, such as IL-8, G-CSF (granulocyte colony stimulating factor), and GM-CSF (granulocyte macrophage colony stimulating factor) which induce neutrophil infiltration and differentiation. Read More

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Alternative regulation of HIF-1α stability through Phosphorylation on Ser451.

Biochem Biophys Res Commun 2021 03 4;545:150-156. Epub 2021 Feb 4.

Tumor Microenvironment Global Core Research Center, College of Pharmacy, Seoul National University, Seoul, South Korea; Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, South Korea; Cancer Research Institute, Seoul National University, Seoul, South Korea. Electronic address:

The hypoxia-inducible factor (HIF-1α) functions as a master regulator of oxygen homeostasis. Oxygen-dependent hydroxylation of HIF-1α is tightly regulated by prolyl hydroxylase domain containing proteins (PHD1, PHD2, and PHD3). The prolyl hydroxylation facilitates the recruitment of the von Hippel-Lindau (VHL) protein, leading to ubiquitination and degradation of HIF-1α by the proteasomes. Read More

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Distal radioulnar joint stabilization with open foveal reinsertion versus tendon graft reconstruction: an experimental study using radiostereometry.

J Exp Orthop 2021 Feb 4;8(1):10. Epub 2021 Feb 4.

Department of Clinical Medicine, Aarhus University, Palle Juul-Jensens Boulevard 165, 8200, Aarhus N, Denmark.

Purpose: Symptomatic instability of the distal radioulnar joint (DRUJ) caused by lesion of the Triangular Fibrocartilage Complex (TFCC) can be treated with a number of surgical techniques. Clinical examination of DRUJ translation is subjective and limited by inter-observer variability. The aim of this study was to compare the stabilizing effect on DRUJ translation with two different surgical methods using the Piano-key test and a new precise low-dose, non-invasive radiostereometric imaging method (AutoRSA). Read More

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February 2021

Soluble/MOF-Supported Palladium Single Atoms Catalyze the Ligand-, Additive-, and Solvent-Free Aerobic Oxidation of Benzyl Alcohols to Benzoic Acids.

J Am Chem Soc 2021 Feb 4;143(6):2581-2592. Epub 2021 Feb 4.

Instituto de Ciencia Molecular (ICMol), Universidad de Valencia, 46980 Paterna, Valencia, Spain.

Metal single-atom catalysts (SACs) promise great rewards in terms of metal atom efficiency. However, the requirement of particular conditions and supports for their synthesis, together with the need of solvents and additives for catalytic implementation, often precludes their use under industrially viable conditions. Here, we show that palladium single atoms are spontaneously formed after dissolving tiny amounts of palladium salts in neat benzyl alcohols, to catalyze their direct aerobic oxidation to benzoic acids without ligands, additives, or solvents. Read More

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February 2021

Activation of NRF2 and ATF4 Signaling by the Pro-Glutathione Molecule I-152, a Co-Drug of -Acetyl-Cysteine and Cysteamine.

Antioxidants (Basel) 2021 Jan 26;10(2). Epub 2021 Jan 26.

Department of Biomolecular Sciences, University of Urbino Carlo Bo, 61029 Urbino, Italy.

I-152 combines two pro-glutathione (GSH) molecules, namely N-acetyl-cysteine (NAC) and cysteamine (MEA), to improve their potency. The co-drug efficiently increases/replenishes GSH levels in vitro and in vivo; little is known about its mechanism of action. Here we demonstrate that I-152 not only supplies GSH precursors, but also activates the antioxidant kelch-like ECH-associated protein 1/nuclear factor E2-related factor 2 (KEAP1/NRF2) pathway. Read More

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January 2021

Loss of Impairs Inosine RNA Modifications in Mouse Oocytes.

Int J Mol Sci 2021 Jan 26;22(3). Epub 2021 Jan 26.

Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS 661601, USA.

Mammalian oocytes must degrade maternal transcripts through a process called translational mRNA decay, in which maternal mRNA undergoes translational activation, followed by deadenylation and mRNA decay. Once a transcript is translationally activated, it becomes deadenylated by the CCR4-NOT complex. Knockout of CCR4-NOT Transcription Complex Subunit 6 Like (), a deadenylase within the CCR4-NOT complex, results in mRNA decay defects during metaphase I (MI) entry. Read More

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January 2021

LARP1 and LARP4: up close with PABP for mRNA 3' poly(A) protection and stabilization.

RNA Biol 2021 02 31;18(2):259-274. Epub 2021 Jan 31.

Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, United States.

La-related proteins (LARPs) share a La motif (LaM) followed by an RNA recognition motif (RRM). Together these are termed the La-module that, in the prototypical nuclear La protein and LARP7, mediates binding to the UUU-3'OH termination motif of nascent RNA polymerase III transcripts. We briefly review La and LARP7 activities for RNA 3' end binding and protection from exonucleases before moving to the more recently uncovered poly(A)-related activities of LARP1 and LARP4. Read More

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February 2021

Giant cell tumor of distal ulna treated using en-bloc resection combined with extensor carpi ulnaris and flexor carpi ulnaris tendon stabilization: A case report.

Int J Surg Case Rep 2021 Feb 20;79:362-367. Epub 2021 Jan 20.

Department of Orthopaedic and Traumatology, Dr. Cipto Mangunkusumo National Central General Hospital - Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.

Introduction: Giant Cell Tumor (GCT) is a form of bone tumor which is rare, benign, and locally invasive. To date, there have not been many case reports regarding cases of GCTs on the distal ulna which made the optimum strategy in management remain controversial. In some reported cases, the patient was treated with wide excision followed by reconstructive procedure resulting in ulnar translation of the carpal bones and dynamic convergence of the ulna towards the radius. Read More

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February 2021

JAK-STAT Pathway Inhibition Partially Restores Intestinal Homeostasis in - and -Intestinal Epithelial Cell-Deficient Mice.

Cells 2021 Jan 23;10(2). Epub 2021 Jan 23.

Département D'immunologie et Biologie Cellulaire, Pavillon de Recherche Appliquée Sur le Cancer, Faculté de Médecine et Des Sciences de la Santé, Université de Sherbrooke, Sherbrooke, QC J1E 4K8, Canada.

We have previously reported that histone deacetylase epigenetic regulator and deletion in intestinal epithelial cells (IEC) disrupts mucosal tissue architecture and barrier, causing chronic inflammation. In this study, proteome and transcriptome analysis revealed the importance of signaling pathways induced upon genetic IEC- and deletion. Indeed, Gene Ontology biological process analysis of enriched deficient IEC RNA and proteins identified common pathways, including lipid metabolic and oxidation-reduction process, cell adhesion, and antigen processing and presentation, related to immune responses, correlating with dysregulation of major histocompatibility complex (MHC) class II genes. Read More

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January 2021

Structural characterization of cystathionine γ-lyase smCSE enables aqueous metal quantum dot biosynthesis.

Int J Biol Macromol 2021 Mar 23;174:42-51. Epub 2021 Jan 23.

Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai 519000, Guangdong, China. Electronic address:

The development and utilization of inorganic material biosynthesis have evolved into single macromolecular systems. A putative cystathionine γ-lyase of bacteria Stenotrophomonas maltophilia (smCSE) is a newly identified biomolecule that enables the synthesis of nanomaterials. Due to the lack of structural information, the mechanism of smCSE biosynthesis remains unclear. Read More

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SIRT7-dependent deacetylation of NPM promotes p53 stabilization following UV-induced genotoxic stress.

Proc Natl Acad Sci U S A 2021 02;118(5)

Department of Cardiac Development and Remodeling, Max Planck Institute for Heart and Lung Research, 61231 Bad Nauheim, Germany;

Adaptation to different forms of environmental stress is crucial for maintaining essential cellular functions and survival. The nucleolus plays a decisive role as a signaling hub for coordinating cellular responses to various extrinsic and intrinsic cues. p53 levels are normally kept low in unstressed cells, mainly due to E3 ubiquitin ligase MDM2-mediated degradation. Read More

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February 2021

Research Progress in the Molecular Functions of Plant mTERF Proteins.

Cells 2021 Jan 21;10(2). Epub 2021 Jan 21.

Instituto de Bioingeniería, Universidad Miguel Hernández, Campus de Elche, 03202 Elche, Spain.

Present-day chloroplast and mitochondrial genomes contain only a few dozen genes involved in ATP synthesis, photosynthesis, and gene expression. The proteins encoded by these genes are only a small fraction of the many hundreds of proteins that act in chloroplasts and mitochondria. Hence, the vast majority, including components of organellar gene expression (OGE) machineries, are encoded by nuclear genes, translated into the cytosol and imported to these organelles. Read More

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January 2021

Geometric modelling of CORA-based levelling osteotomy in the dog.

Res Vet Sci 2021 Mar 6;135:127-133. Epub 2021 Jan 6.

Department of Veterinary Clinical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Dyrlægevej 16, 1870 Frederiksberg, Denmark. Electronic address:

Centre of rotation of angulation (CORA)-based levelling osteotomy (CBLO) is a recent addition to surgical procedures for stabilization of the cranial cruciate ligament-deficient canine stifle joint. Careful identification of the CORA location preoperatively and use of this location intraoperatively are required to ensure accurate correction of the tibial plateau angle. Limited data are available regarding the magnitude and source of potential errors during planning and execution of CBLO. Read More

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Fabrication of Pickering emulsion based on particles combining pectin and zein: Effects of pectin methylation.

Carbohydr Polym 2021 Mar 16;256:117515. Epub 2020 Dec 16.

School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address:

Although the stabilization of Pickering emulsions based on the interaction of pectin and zein is known to be effective, the effects of the degree of methylation (DM) of pectin on the stability of Pickering emulsions remains unclear. In the present study, pectin/zein particle dispersions (PZDs) were fabricated by anti-solvent precipitation based on different DM values of pectin (13.70 %-71. Read More

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The Role of lncRNAs in Gene Expression Regulation through mRNA Stabilization.

Noncoding RNA 2021 Jan 5;7(1). Epub 2021 Jan 5.

Biocruces Bizkaia Health Research Institute, 48903 Barakaldo, Spain.

mRNA stability influences gene expression and translation in almost all living organisms, and the levels of mRNA molecules in the cell are determined by a balance between production and decay. Maintaining an accurate balance is crucial for the correct function of a wide variety of biological processes and to maintain an appropriate cellular homeostasis. Long non-coding RNAs (lncRNAs) have been shown to participate in the regulation of gene expression through different molecular mechanisms, including mRNA stabilization. Read More

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January 2021

Structural Insights into the Interaction of Clinically Relevant Phosphorothioate mRNA Cap Analogs with Translation Initiation Factor 4E Reveal Stabilization via Electrostatic Thio-Effect.

ACS Chem Biol 2021 02 13;16(2):334-343. Epub 2021 Jan 13.

Centre of New Technologies, University of Warsaw, Banacha 2c, 02-097 Warsaw, Poland.

mRNA-based therapies and vaccines constitute a disruptive technology with the potential to revolutionize modern medicine. Chemically modified 5' cap structures have provided access to mRNAs with superior translational properties that could benefit the currently flourishing mRNA field. Prime examples of compounds that enhance mRNA properties are antireverse cap analog diastereomers that contain an O-to-S substitution within the β-phosphate (β-S-ARCA D1 and D2), where D1 is used in clinically investigated mRNA vaccines. Read More

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February 2021

The pentatricopeptide repeat protein PGR3 is required for the translation of petL and ndhG by binding their 5'UTRs.

Plant Cell Physiol 2021 Jan 13. Epub 2021 Jan 13.

Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan.

PGR3 is a P-class pentatricopeptide repeat (PPR) protein required for the stabilization of petL operon RNA and the translation of the petL gene in plastids. Irrespective of its important roles in plastids, key questions have remained unanswered, including how PGR3 protein promotes translation and which plastid mRNAs PGR3 activates the translation. Here, we show that PGR3 facilitates the translation from ndhG, in addition to petL, through binding to their 5' untranslated regions (UTRs). Read More

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January 2021

Predictive Factors Affecting Surgical Outcomes in Patients with Degenerative Lumbar Spondylolisthesis.

Spine (Phila Pa 1976) 2021 05;46(9):610-616

Department of Orthopaedics, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.

Study Design: Post-hoc analysis of 5-year follow-up data from a prospective randomized multicenter trial.

Objective: The purpose of this study was to identify preoperative factors that predict poor postoperative outcomes and define clinically important abnormal instabilities in degenerative lumbar spondylolisthesis.

Summary Of Background Data: Current evidence regarding prognostic factors affecting clinical outcomes after surgery for degenerative lumbar spondylolisthesis is still limited. Read More

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Biplane Fluoroscopic-Guided Percutaneous Thoracic Instrumentation: A Technical Note.

Cureus 2020 Dec 6;12(12):e11939. Epub 2020 Dec 6.

Neurosurgery, Baylor Scott & White Medical Center, Temple, USA.

Biplane fluoroscopy in a hybrid operating room (OR) is commonly used for neuroendovascular and hybrid open/endovascular cases. The image quality is far superior to most C-arm fluoroscopy machines in the regular OR. This advantage can be particularly useful for upper and mid-thoracic percutaneous screw placement because the C-arm visualization in the regular OR is suboptimal due to shoulders absorbing the majority of the photons on lateral fluoroscopy. Read More

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December 2020

Two-Dimensional Visualization of the Three-Dimensional Planned Sacroiliac Screw Corridor with the Slice Fusion Method.

J Clin Med 2021 Jan 6;10(2). Epub 2021 Jan 6.

Department of Trauma Surgery, Regensburg University Medical Center, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany.

Insertion of sacro-iliac (SI) screws for stabilization of the posterior pelvic ring without intraoperative navigation or three-dimensional imaging can be challenging. The aim of this study was to develop a simple method to visualize the ideal SI screw corridor, on lateral two-dimensional images, corresponding to the lateral fluoroscopic view, used intraoperatively while screw insertion, to prevent neurovascular injury. We used multiplanar reconstructions of pre- and postoperative computed tomography scans (CT) to determine the position of the SI corridor. Read More

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January 2021

Do We Really Need to Worry About Calcaneocuboid Subluxation During Lateral Column Lengthening for Planovalgus Foot Deformity?

J Pediatr Orthop 2021 Mar;41(3):e246-e251

Texas Scottish Rite Hospital for Children, Dallas, TX.

Background: Although lengthening of the lateral column through an osteotomy of the anterior calcaneus is an integral component of flatfoot reconstruction in younger patients with flexible planovalgus deformities, the procedure has been implicated in iatrogenic calcaneocuboid (CC) subluxation and subsequent degenerative changes at the CC articulation. The purpose of this study is to characterize alterations at the CC joint after lateral column lengthening (LCL) and determine if Steinmann pin stabilization of the CC joint before distraction maintains a normal relationship.

Methods: Seven matched pairs of fresh-frozen cadaveric feet underwent preprocedure plain radiography and cross-sectional computed tomography (CT) imaging. Read More

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Essential biochemical, biophysical and computational inputs on efficient functioning of Mycobacterium tuberculosis HRv FtsY.

Int J Biol Macromol 2021 Feb 5;171:59-73. Epub 2021 Jan 5.

CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi 110007, India; Academy of Scientific and Innovative Research (AcSIR), CSIR-HRDC, Ghaziabad, Uttar Pradesh 201 002, India. Electronic address:

Mycobacterium tuberculosis (M. tuberculosis HRv) utilizes the signal recognition particle pathway (SRP pathway) system for secretion of various proteins from ribosomes to the extracellular surface which plays an important role in the machinery running inside the bacterium. This system comprises of three major components FtsY, FfH and 4. Read More

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February 2021

Novel Strategies for Endothelial Preservation in Lung Transplant Ischemia-Reperfusion Injury.

Front Physiol 2020 18;11:581420. Epub 2020 Dec 18.

Department of Thoracic Surgery, University Hospital Freiburg, Freiburg, Germany.

Lung ischemia reperfusion (IR) injury inevitably occurs during lung transplantation. The pulmonary endothelium is the primary target of IR injury that potentially results in severe pulmonary dysfunction. Over the last decades, various molecules, receptors, and signaling pathways were identified in order to develop treatment strategies for the preservation of the pulmonary endothelium against IR injury. Read More

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December 2020