509,018 results match your criteria stability testingresults

Nse5/6 is a negative regulator of the ATPase activity of the Smc5/6 complex.

Nucleic Acids Res 2021 Apr 13. Epub 2021 Apr 13.

Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Falmer, UK.

The multi-component Smc5/6 complex plays a critical role in the resolution of recombination intermediates formed during mitosis and meiosis, and in the cellular response to replication stress. Using recombinant proteins, we have reconstituted a series of defined Saccharomyces cerevisiae Smc5/6 complexes, visualised them by negative stain electron microscopy, and tested their ability to function as an ATPase. We find that only the six protein 'holo-complex' is capable of turning over ATP and that its activity is significantly increased by the addition of double-stranded DNA to reaction mixes. Read More

View Article and Full-Text PDF

Microstructural features, spectroscopic study and thermal analysis of potassium permanganate filled PVA-PVP blend films.

G Veena Blaise Lobo

J Phys Condens Matter 2021 Apr 13. Epub 2021 Apr 13.

Department of Physics, Karnatak University, Karnatak Science College, Dharwad 580 001, Karnataka, Dharwad, 580001, INDIA.

Potassium permanganate (KMnO4) filled polyvinyl alcohol (PVA) - polyvinyl pyrrolidone (PVP) polymeric blend films have been prepared by solution casting technique, with filler levels (FL) varying from 0.01 up to 4.70 mass %. Read More

View Article and Full-Text PDF

Tissue printing for engineering transplantable human parathyroid patch to improves parathyroid engraftment, integration, and hormone secretion.

Biofabrication 2021 Apr 13. Epub 2021 Apr 13.

Seoul National University Bundang Hospital, bundang-gu, Seongnam, 13620, Korea (the Republic of).

During thyroid surgery, some parathyroid glands fail to maintain their function, therefore, they are unavoidably detached from the patient. For the purpose of re-preservation of the function, they are minced into small segments and transplanted into the fat or muscle layer. Yet, this method of auto-grafting the parathyroid glands is frequently unsuccessful due to its poor interaction and engraftment with the native tissue, eventually leading to the dysfunction of the parathyroid hormone (PTH) secretion. Read More

View Article and Full-Text PDF

Dual activation of Hedgehog and Wnt/β-catenin signaling pathway caused by downregulation of SUFU targeted by miRNA-150 in human gastric cancer.

Aging (Albany NY) 2021 Apr 12;13. Epub 2021 Apr 12.

Guangdong Key Laboratory for Genome Stability and Disease Prevention, Department of Pathology, Shenzhen University School of Medicine, Shenzhen 518060, Guangdong, P.R. China.

Mounting evidence has shown that miRNA-150 expression is upregulated in gastric cancer (GC) and is associated with gastric carcinogenesis, but the underlying oncogenic mechanism remains elusive. Here, we discovered that miRNA-150 targets the tumor suppressor SUFU to promote cell proliferation, migration, and the epithelial-mesenchymal transition (EMT) via the dual activation of Hedgehog (Hh) and Wnt signaling. MiRNA-150 was highly expressed in GC tissues and cell lines, and the level of this miRNA was negatively related to that of SUFU. Read More

View Article and Full-Text PDF

Unprecedented saturation limit achieved by inorganic polycationic cluster (SbTe) for light noble gases (He & Ne).

J Mol Graph Model 2021 Mar 27;106:107910. Epub 2021 Mar 27.

Department of Chemistry, COMSATS University, Abbottabad Campus, KPK, 22060, Pakistan. Electronic address:

Among noble gases, Helium and Neon have smaller size, high ionization potential and low polarizability due to which these two gases exhibit weak binding affinities toward any surface. Bartlet's discovery and subsequent similar successful discoveries of stable complexes of noble gases opened new avenues for the storage of noble gases particularly on the new surfaces and their interactions for the storage of these gases. Here, we report the adsorption of light noble gases on polycationic clusters. Read More

View Article and Full-Text PDF

Matairesinol, an active constituent of HC9 polyherbal formulation, exhibits HDAC8 inhibitory and anticancer activity.

Biophys Chem 2021 Apr 2;273:106588. Epub 2021 Apr 2.

Cancer Research Lab., Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth Deemed to be University, Pune-Satara Road, Pune 411043, Maharashtra, India. Electronic address:

Histone deacetylase 8 (HDAC8) has emerged as a promising drug target for cancer therapeutics development. HDAC8 has been reported to regulate cancer cell proliferation, invasion and promote metastasis through modulation of cell cycle associated proteins. Of late, phytocompounds have been demonstrated to exhibit anticancer and anti-HDAC8 activity. Read More

View Article and Full-Text PDF

Preparation and characterization of octenyl succinylated starch microgels via a water-in-oil (W/O) inverse microemulsion process for loading and releasing epigallocatechin gallate.

Food Chem 2021 Mar 23;355:129661. Epub 2021 Mar 23.

State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China; Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt; Department of Medical Pharmacology, Medical Faculty, Ataturk University, Erzurum, Turkey. Electronic address:

Corn starch (CS), octenyl succinic anhydride modified corn starch (OSCS) and shells (OSC) microgels have been prepared using water-in-oil (W/O) inverse microemulsions for loading and releasing of epigallocatechin gallate (EGCG). The structural and morphological properties of CS, OSCS, and OSC microgels were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), Transmission electron microscopy (TEM), and Thermogravimetric analysis (TGA). The strong hydrogen bonds between starch molecules in the W/O system and interplay between hydroxyl groups of EGCG and oxygen atoms of starch microgels were formed. Read More

View Article and Full-Text PDF

Polypyrrole and enzyme free cholesterol flakes based composite: Selective determination of theophylline.

J Pharm Biomed Anal 2021 Apr 6;199:114065. Epub 2021 Apr 6.

Polymer Electronics Lab, Department of Bioelectronics and Biosensors, Alagappa University, Karaikudi, 630 003, Tamil Nadu, India. Electronic address:

In the present work, we demonstrated the non-enzymatic theophylline (TP) detection using nanostructured Polypyrrole (Ppy) and Cholesterol (Ch) flakes coordinated nanocomposite coated on Glassy Carbon Electrode. The higher active surface area and good mechanical stability of the Ppy integrated Ch composite exhibited better oxidation towards TP. The electron transfer across hydrogen bonding is of fundamental importance in enhancing the biochemical process in this biosensor system. Read More

View Article and Full-Text PDF

The sub-lethal impact of plastic and tire rubber leachates on the Mediterranean mussel Mytilus galloprovincialis.

Environ Pollut 2021 Apr 4;283:117081. Epub 2021 Apr 4.

University of Bologna, Department of Biological, Geological, and Environmental Sciences, Via Sant'Alberto 163, 48123, Ravenna, Italy. Electronic address:

Ocean contamination by synthetic polymers can represent a risk for the fitness of marine species due to the leaching of chemical additives. This study evaluated the sub-lethal effects of plastic and rubber leachates on the mussel Mytilus galloprovincialis through a battery of biomarkers encompassing lysosomal endpoints, oxidative stress/detoxification parameters, and specific responses to metals/neurotoxicants. Mussels were exposed for 7 days to leachates from car tire rubber (CTR), polypropylene (PP), polyethylene terephthalate (PET), polystyrene (PS) and polyvinyl chloride (PVC), containing organic additives and metals in the ng-μg/L range. Read More

View Article and Full-Text PDF

Land cover alteration shifts ecological assembly processes in floodplain lakes: Consequences for fish community dynamics.

Sci Total Environ 2021 Mar 25;782:146724. Epub 2021 Mar 25.

School of Ecology and Nature Conservation, Beijing Forestry University, Beijing 100083, China; Science Division, NSW Department of Planning, Industry and Environment, Sydney 2124, Australia. Electronic address:

Habitat degradation is expected to alter community structure and consequently, ecosystem functions including the maintenance of biodiversity. Understanding the underlying abiotic and biotic assembly mechanisms controlling temporal and spatial community structure and patterns is a central issue in biodiversity conservation. In this study, using monthly time series of fish abundance data collected over a three-year period, we compared the temporal community dynamics in natural habitats and poplar plantations in one of the largest river-lake floodplain ecosystems in China, the Dongting Lake. Read More

View Article and Full-Text PDF

Habitat heterogeneity helps to mitigate pollinator nectar sugar deficit and discontinuity in an agricultural landscape.

Sci Total Environ 2021 Apr 6;782:146909. Epub 2021 Apr 6.

Department of Botany, Mycology, and Ecology, Maria Curie-Sklodowska University, 19 Akademicka St., 20-033 Lublin, Poland. Electronic address:

The scarcity of floral resources and their seasonal discontinuity are considered as major factors for pollinator decline in intensified agricultural landscapes worldwide. The consequences are detrimental for the stability of the environment and ecosystems. Here, we quantified the production of nectar sugars in plant species occurring in man-made, non-cropped areas (non-forest woody vegetation, road verges, railway embankments, field margins, fallow areas) of an agricultural landscape in SE Poland. Read More

View Article and Full-Text PDF

Long-term in situ bioelectrochemical monitoring of biohythane process: Metabolic interactions and microbial evolution.

Bioresour Technol 2021 Apr 7;332:125119. Epub 2021 Apr 7.

Laboratory of Environment-Enhancing Energy (E2E), Key Laboratory of Agricultural Engineering in Structure and Environment, Ministry of Agriculture, College of Water Resources and Civil Engineering, China Agricultural University, Beijing 100083, China. Electronic address:

Microbial stability and evolution are a critical aspect for biosensors, especially in detecting dynamic and emerging anaerobic biohythane production. In this study, two upflow air-cathode chamber microbial fuel cells (UMFCs) were developed for in situ monitoring of the biohydrogen and biomethane reactors under a COD range of 1000-6000 mg/L and 150-1000 mg/L, respectively. Illumina MiSeq sequencing evidenced the dramatic shift of dominant microbial communities in UMFCs from hydrolytic and acidification bacteria (Clostridiaceae_1, Ruminococcaceae, Peptostreptococcaceae) to acetate-oxidizing bacteria (Synergistaceae, Dysgonomonadaceae, Spirochaetaceae). Read More

View Article and Full-Text PDF

Impact of fungal pellets dosage on long-term stability of aerobic granular sludge.

Bioresour Technol 2021 Apr 4;332:125106. Epub 2021 Apr 4.

State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, PR China.

The effects of fungal pellets (FPs) dosage on both structural and functional stability of aerobic granular sludge (AGS) were investigated during 200-day operation. Results showed that the AGS induced by low (a dry mass ratio of FPs to seed sludge, 30%) and high FPs dosage (60%) exhibited good morphology integrity during the entire phase of operation, while the filamentous overgrowth and AGS breakup were observed in the control reactor (0% FPs). Moreover, the granules developed at high FPs dosage demonstrated excellent nutrients removal (COD: 93%; NH4-N: 100%; TN: 77%) and stable bioactivity with a maximum specific oxygen uptake rate (SOUR) of 52. Read More

View Article and Full-Text PDF

Highly-efficient and stable photocatalytic activity of lead-free CsAgInCl double perovskite for organic pollutant degradation.

J Colloid Interface Sci 2021 Mar 29;596:376-383. Epub 2021 Mar 29.

Key Laboratory of Material Physics, Ministry of Education, School of Physics and Microelectronics, Zhengzhou University, Zhengzhou 450052, PR China.

Photocatalytic applications of halide perovskites have attracted increasing attention. However, lack of stability and lead toxicity of lead halide perovskites have hindered their applications. Metal halide double perovskite (DP) CsAgInCl is a stable, environment-friendly semiconductor with direct band gap, and then the best promising alternative to lead halide perovskites. Read More

View Article and Full-Text PDF

New quinolone derivatives as neuropeptide S receptor antagonists: Design, synthesis, homology modeling, dynamic simulations and modulation of Gq/Gs signaling pathways.

Bioorg Chem 2021 Mar 10;111:104817. Epub 2021 Mar 10.

Pharmaceutical Chemistry Department, Faculty of Pharmacy, Helwan University, Ein Helwan, Cairo, P.O. Box 11795, Egypt.

In a search for new neuropeptide S receptor antagonists, we have described a new series of quinolone-pyranopyrimidine hybrid derivatives aiming to modify the inhibitory characters towards NPSR to develop new therapeutic strategies against anxiety, addiction and food disorders. We identified six potent antagonists 3, 4b, 6, 8, 9 and 10 which counteracted the stimulatory effect of NPS at both Gq and Gs pathways, at low micromolar concentrations, through modulation of Ca and cAMP signaling, respectively. Molecular docking predicted the orientation mode of the top active compounds; 10 and 4b with ΔG value of -23. Read More

View Article and Full-Text PDF

Comparison of bicortical, miniplate and hybrid fixation techniques in mandibular advancement and counterclockwise rotation: A finite element analysis study.

J Stomatol Oral Maxillofac Surg 2021 Apr 10. Epub 2021 Apr 10.

Professor, Marmara University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Istanbul, Turkey. Electronic address:

Objective: This study aims to evaluate biomechanical stability and stress distribution of five different fixation types with finite element analysis using 10-mm advancement with or without counterclockwise rotation of the mandible.

Materials And Methods: After sagittal split osteotomy, 10-mm advancement was performed in the first group and 10-mm advancement and 10-degree counterclockwise rotation were performed in the second group. One miniplate (M-1), two-miniplate (M-2), one miniplate and a bicortical screw (H), L-shaped bicortical screw (B-1), and inverted L-shaped bicortical screw (B-2) systems were placed. Read More

View Article and Full-Text PDF

Adsorption-photocatalysis performance of polyaniline/dicarboxyl acid cellulose@graphene oxide for dye removal.

Int J Biol Macromol 2021 Apr 10. Epub 2021 Apr 10.

State Key Laboratory of Biobased Material and Green Papermaking, School of Environmental Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong Province 250353, PR China.

A novel 3-D biopolymer-based adsorption-photocatalytic composite, polyaniline/dicarboxyl acid cellulose@graphene oxide was synthesized and was employed to remove the reactive brilliant red K-2G from aqueous solution. The addition of dicarboxyl acid cellulose could improve the morphology, structure, stability and dispersity of the nanocomposite, thus providing excellent adsorption and photocatalysis performance to the product. Batch of experiments were conducted in two scenarios: adsorption followed by photocatalysis process and simultaneous adsorption-photocatalysis process. Read More

View Article and Full-Text PDF

Profiling of non-polar lipids in tears of contact lens wearers during the day.

Exp Eye Res 2021 Apr 10:108567. Epub 2021 Apr 10.

School of Optometry and Vision Science, University of New South Wales, Sydney, Australia. Electronic address:

Purpose: This study explored whether the non-polar lipids in the human tear fluid lipidome show diurnal variation with and without contact lens wear. It also addressed the relationship between changes in ocular comfort during the day with the level of non-polar lipids.

Methods: Tear samples were collected in the morning and evening with and without contact lenses using fine glass capillary tubes and were analysed by chip-based nano-electrospray ionization tandem mass spectrometric techniques. Read More

View Article and Full-Text PDF

Insects-plants-pathogens: toxicity, dependence and defense dynamics.

Toxicon 2021 Apr 10. Epub 2021 Apr 10.

College of Agriculture and Marine Sciences, Sultan Qaboos University, Muscat. Sultanate of Oman.

In a natural ecosystem, the pathogen-plant-insect relationship has diverse implications for each other. The pathogens as well as insect-pests consume plant tissues as their feed that mostly results in damage. In turn, plant species have evolved specialized defense system to not only protect themselves but reduce the damage also. Read More

View Article and Full-Text PDF

Glucose makes T lose their temper.

Cancer Cell 2021 Apr;39(4):460-462

The Bloomberg-Kimmel Institute for Cancer Immunotherapy at Johns Hopkins, Baltimore, MD 21287, USA. Electronic address:

Competition for glucose regulates the balance between cancer and immune responses. New findings published in Nature show that regulatory T cells (T) shape their metabolism to avoid glucose competition, thus maintaining their stability and sustaining tumor progression. This research suggests hijacking the "eating habits" of T could improve cancer therapy. Read More

View Article and Full-Text PDF

Donor cell memory confers a metastable state of directly converted cells.

Cell Stem Cell 2021 Apr 7. Epub 2021 Apr 7.

Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Münster 48149, Germany; Faculty of Medicine, University of Münster, Münster 48149, Germany. Electronic address:

Generation of induced oligodendrocyte progenitor cells (iOPCs) from somatic fibroblasts is a strategy for cell-based therapy of myelin diseases. However, iOPC generation is inefficient, and the resulting iOPCs exhibit limited expansion and differentiation competence. Here we overcome these limitations by transducing an optimized transcription factor combination into a permissive donor phenotype, the pericyte. Read More

View Article and Full-Text PDF

Mobile Protons Limit the Stability of Salt Bridges in the Gas Phase: Implications for the Structures of Electrosprayed Protein Ions.

J Phys Chem B 2021 Apr 13. Epub 2021 Apr 13.

Department of Chemistry, The University of Western Ontario, London, Ontario N6A 5B7, Canada.

Electrosprayed protein ions can retain native-like conformations. The intramolecular contacts that stabilize these compact gas-phase structures remain poorly understood. Recent work has uncovered abundant salt bridges in electrosprayed proteins. Read More

View Article and Full-Text PDF

The Prevalence of Adding-On or Distal Junctional Kyphosis in Adolescent Idiopathic Scoliosis Treated By Anterior Spinal Fusion to L3 was Significantly Higher Than By Posterior Spinal Fusion to L3.

Neurospine 2021 Apr 12. Epub 2021 Apr 12.

Department of Orthopaedic Surgery, Washington University School of Medicine, St. Louis, St. Louis, United States.

Objective: To compare and identify risk factors for distal adding on (AO) or distal junctional kyphosis (DJK) in adolescent idiopathic scoliosis (AIS) treated by anterior- (ASF) and posterior spinal fusion (PSF) to L3.

Methods: AIS patients undergoing ASF vs. PSF to L3 from 2000-2010 were analyzed. Read More

View Article and Full-Text PDF

Puerarin dry powder inhaler formulations for pulmonary delivery: Development and characterization.

PLoS One 2021 13;16(4):e0249683. Epub 2021 Apr 13.

Queensland University of Technology, Pharmacy Discipline, School of Clinical Sciences, Faculty of Health, Brisbane, Queensland, Australia.

This study aims at developing and characterizing the puerarin dry powder inhaler (DPI) formulations for pulmonary delivery. The inhalable particles size (<2 μm) was accomplished by micronization and its morphology was examined by scanning electron microscopy (SEM). The puerarin-excipient interaction in powder mixtures was analyzed by using Fourier transform infrared spectroscopy (FTIR), Raman confocal microscopy, X-Ray powder Diffraction (XRD), and differential scanning calorimetry (DSC) methods. Read More

View Article and Full-Text PDF

The Use of Generalizability Theory to Inform Sampling of Educator Language Used With Preschoolers With Autism Spectrum Disorder.

J Speech Lang Hear Res 2021 Apr 13:1-10. Epub 2021 Apr 13.

Department of Educational Psychology, University of Minnesota, Twin Cities, Minneapolis.

Purpose A myriad features can impact the nature, frequency, and length of adult-child interactions important for language learning. Empirical investigations of language learning opportunities for young children with autism spectrum disorder (ASD) provide limited generalizable insight, with inferences more constrained to the sample than is often considered. The aim of this study was to explore a multidimensional understanding of reliability and define optimal measurement procedures for a measurement approach used to examine the language of preschool educators interacting with children with ASD. Read More

View Article and Full-Text PDF

Three Simple Properties Explain Protein Stability Change upon Mutation.

J Chem Inf Model 2021 Apr 13. Epub 2021 Apr 13.

DTU Chemistry, Technical University of Denmark, Building 206, 2800 Kgs. Lyngby, Denmark.

Accurate prediction of protein stability upon mutation enables rational engineering of new proteins and insights into protein evolution and monogenetic diseases caused by single-point amino acid substitutions. Many tools have been developed to this aim, ranging from energy-based models to machine-learning methods that use large amounts of experimental data. However, as the methods become more complex, the interpretation of the chemistry underlying the protein stability effects becomes obscure. Read More

View Article and Full-Text PDF

Bcl-xL Dynamics under the Lens of Protein Structure Networks.

J Phys Chem B 2021 Apr 13. Epub 2021 Apr 13.

Computational Biology Laboratory, Danish Cancer Society Research Center, 2100 Copenhagen, Denmark.

Understanding the finely orchestrated interactions leading to or preventing programmed cell death (apoptosis) is of utmost importance in cancer research because the failure of these systems could eventually lead to the onset of the disease. In this regard, the maintenance of a delicate balance between the promoters and inhibitors of mitochondrial apoptosis is crucial, as demonstrated by the interplay among the Bcl-2 family members. In particular, B-cell lymphoma extra-large (Bcl-x) is a target of interest due to the forefront role of its dysfunctions in cancer development. Read More

View Article and Full-Text PDF

Computational Saturation Screen Reveals the Landscape of Mutations in Human Fumarate Hydratase.

J Chem Inf Model 2021 Apr 13. Epub 2021 Apr 13.

Department of Medical Physics and Biomedical Engineering, UCL, London WC1E 6BT, U.K.

Single amino acid substitutions within protein structures often manifest with clinical conditions in humans. The mutation of a single amino can significantly alter protein folding and stability, or change protein dynamics to influence function. The chemical engineering field has developed a large toolset for predicting the influence of point mutations with the aim of guiding the design of improved and more stable proteins. Read More

View Article and Full-Text PDF

Core-Shell Tecto Dendrimers Enable Enhanced Tumor MR Imaging through an Amplified EPR Effect.

Biomacromolecules 2021 Apr 13. Epub 2021 Apr 13.

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, People's Republic of China.

Development of nanoplatforms that can amplify the passive tumor targeting effect based on enhanced permeability and retention (EPR) effect is crucial for precision cancer nanomedicine applications. Herein, we present the development of core-shell tecto dendrimers (CSTDs) as a platform for enhanced tumor magnetic resonance (MR) imaging through an amplified EPR effect. In this work, poly(amidoamine) (PAMAM) dendrimers of generation 5 (G5) were decorated with β-cyclodextrin (CD) and then assembled with G3 PAMAM dendrimers premodified with adamantane (Ad) via supramolecular recognition of CD and Ad. Read More

View Article and Full-Text PDF

Superior Conversion Efficiency Achieved in GeP/h-BN Heterostructures as Novel Flexible and Ultralight Thermoelectrics.

ACS Appl Mater Interfaces 2021 Apr 13. Epub 2021 Apr 13.

Laboratory of High Pressure Physics and Material Science (HPPMS), School of Physics and Physical Engineering, Qufu Normal University, Qufu, Shandong 273165, China.

GeP materials are attracting broad research interest due to their typical puckered layer structure, high carrier mobility, and chemical stability. This peculiarity expedites the independent control of anisotropic electrical and thermal conductance, which is thus expected to possess great thermoelectric potential. Nevertheless, the metal characteristics of GeP in the bulk and thick films are adverse to real application because of the low Seebeck coefficient. Read More

View Article and Full-Text PDF