28,205 results match your criteria stability multiple


Multifunctional Graphdiyne-Cerium Oxide Nanozymes Facilitate MicroRNA Delivery and Attenuate Tumor Hypoxia for Highly Efficient Radiotherapy of Esophageal Cancer.

Adv Mater 2021 May 5:e2100556. Epub 2021 May 5.

CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China, Beijing, 100190, China.

Radioresistance is an important challenge for clinical treatments. The main causes of radioresistance include hypoxia in the tumor microenvironment, the antioxidant system within cancer cells, and the upregulation of DNA repair proteins. Here, a multiple radiosensitization strategy of high-Z-element-based radiation enhancement is designed, attenuating hypoxia and microRNA therapy. Read More

View Article and Full-Text PDF

Learning-Dependent Dendritic Spine Plasticity Is Impaired in Spontaneous Autoimmune Encephalomyelitis.

Dev Neurobiol 2021 May 4. Epub 2021 May 4.

Department of Anesthesiology, New York University School of Medicine, New York, NY, USA.

Cognitive impairment is often observed in multiple sclerosis and its animal models, experimental autoimmune encephalomyelitis (EAE). Using mice with immunization-induced EAE, we have previously shown that the stability of cortical synapses is markedly decreased before the clinical onset of EAE. In this study, we examined learning-dependent structural synaptic plasticity in a spontaneous EAE model. Read More

View Article and Full-Text PDF

Intestinal proteases profiling from Anticarsia gemmatalis and their binding to inhibitors.

Arch Insect Biochem Physiol 2021 May 5:e21792. Epub 2021 May 5.

Department of Biochemistry and Molecular Biology, Laboratory of Enzymology and Biochemistry of Proteins and Peptides, Universidade Federal de Viçosa, UFV, BIOAGRO/INCT-IPP, Viçosa, Minas Gerais, Brazil.

Although the importance of intestinal hydrolases is recognized, there is little information on the intestinal proteome of lepidopterans such as Anticarsia gemmatalis. Thus, we carried out the proteomic analysis of the A. gemmatalis intestine to characterize the proteases by LC/MS. Read More

View Article and Full-Text PDF

The tumour suppressor brain tumour (Brat) regulates linker histone dBigH1 expression in the female germline and the early embryo.

Open Biol 2021 May 5;11(5):200408. Epub 2021 May 5.

Institute of Molecular Biology of Barcelona, CSIC, Barcelona 08028, Spain.

Linker histones H1 are essential chromatin components that exist as multiple developmentally regulated variants. In metazoans, specific H1s are expressed during germline development in a tightly regulated manner. However, the mechanisms governing their stage-dependent expression are poorly understood. Read More

View Article and Full-Text PDF

Submillimetre mechanistic designs of termite-built structures.

J R Soc Interface 2021 May 5;18(178):20200957. Epub 2021 May 5.

School of Civil Engineering, The University of Sydney, 2006, Sydney, Australia.

Termites inhabit complex underground mounds of intricate stigmergic labyrinthine designs with multiple functions as nursery, food storage and refuge, while maintaining a homeostatic microclimate. Past research studied termite building activities rather than the actual material structure. Yet, prior to understanding how multi-functionality shaped termite building, a thorough grasp of submillimetre mechanistic architecture of mounds is required. Read More

View Article and Full-Text PDF

Systematic Review: The Gut Microbiome and Its Potential Clinical Application in Inflammatory Bowel Disease.

Microorganisms 2021 Apr 30;9(5). Epub 2021 Apr 30.

Hospital Universitario de La Princesa, Instituto de Investigación Sanitaria Princesa (IIS-IP), Universidad Autónoma de Madrid, 28006 Madrid, Spain.

Inflammatory bowel disease (IBD) is a chronic relapsing-remitting systemic disease of the gastrointestinal tract. It is well established that the gut microbiome has a profound impact on IBD pathogenesis. Our aim was to systematically review the literature on the IBD gut microbiome and its usefulness to provide microbiome-based biomarkers. Read More

View Article and Full-Text PDF

Anthocyanins Recovered from Agri-Food By-Products Using Innovative Processes: Trends, Challenges, and Perspectives for Their Application in Food Systems.

Molecules 2021 Apr 30;26(9). Epub 2021 Apr 30.

Department of Food and Nutrition, School of Food Engineering, University of Campinas, Monteiro Lobato Street 80, Campinas 13083-862, Brazil.

Anthocyanins are naturally occurring phytochemicals that have attracted growing interest from consumers and the food industry due to their multiple biological properties and technological applications. Nevertheless, conventional extraction techniques based on thermal technologies can compromise both the recovery and stability of anthocyanins, reducing their global yield and/or limiting their application in food systems. The current review provides an overview of the main innovative processes (e. Read More

View Article and Full-Text PDF

Multivalent Amide-Hydrogen-Bond Supramolecular Binder Enhances the Cyclic Stability of Silicon-Based Anodes for Lithium-Ion Batteries.

ACS Appl Mater Interfaces 2021 May 4. Epub 2021 May 4.

College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.

A supramolecular polymer, poly(-acryloyl glycinamide) (PNAGA), with a bisamide group on each side of the chain forming multiple amide-hydrogen bonds was synthesized in this work as a binder for silicon (Si)-based anodes. This supramolecular polymer binder with improved mechanical properties presents good interfacial adhesion with Si particles forming hydrogen bonds and enhances the adhesive strength between the electrode material film and the copper current collector. Benefiting from the highly stable inter- and intramolecular multiple amide-hydrogen bonds of the PNAGA binder, the electrode structure maintains integrity and a stable solid electrolyte interphase (SEI) layer is formed on the surface of Si particles. Read More

View Article and Full-Text PDF

Flexible integrated sensing platform for monitoring wound temperature and predicting infection.

Microb Biotechnol 2021 May 4. Epub 2021 May 4.

Department of Burn and Plastic Surgery, Second Affiliated Hospital of Air Force Military Medical University, Xi'an, 710000, China.

Wound infection is a challenging clinical problem that imposes substantial economic and psychological burdens on patients. However, the wound covered by a dressing is in an 'unknown' state. Recently, researchers have focused on understanding the condition of the wound without removing the dressing. Read More

View Article and Full-Text PDF

Test-retest reliability of tone- and 40 Hz train-evoked gamma oscillations in female rats and their sensitivity to low-dose NMDA channel blockade.

Psychopharmacology (Berl) 2021 May 4. Epub 2021 May 4.

Department of Pharmaceutical Sciences, Bill Gatton College of Pharmacy, East Tennessee State University, VA Building 7, Room 324, Maple Ave, Johnson City, TN, 37604, USA.

Rationale: Schizophrenia patients consistently show deficits in sensory-evoked broadband gamma oscillations and click-evoked entrainment at 40 Hz, called the 40-Hz auditory steady-state response (ASSR). Since such evoked oscillations depend on cortical N-methyl D-aspartic acid (NMDA)-mediated network activity, they can serve as pharmacodynamic biomarkers in the preclinical and clinical development of drug candidates engaging these circuits. However, there are few test-retest reliability data in preclinical species, a prerequisite for within-subject testing paradigms. Read More

View Article and Full-Text PDF

Unveiling the phase behavior of CE non-ionic surfactants in water through coarse-grained molecular dynamics simulations.

Soft Matter 2021 May 4. Epub 2021 May 4.

CICECO - Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-1933, Aveiro, Portugal.

Poly(oxyethylene) alkyl ethers, usually denoted by CiEj surfactants, exhibit a rich phase behavior in water, self-assembling to form a variety of 3-D structures with a controllable morphology that find multiple applications across different industrial segments. Hence, being able to describe and understand the effect of molecular structure on the phase behavior of these systems is highly relevant for the efficient design of new materials and their applications. Considering the promising results obtained over the last decade using the MARTINI model to describe ethylene-oxide containing compounds, an extensive assessment of the ability of such a model to describe the phase behavior of CiEj in water was carried out and results are presented here. Read More

View Article and Full-Text PDF

Enhancement of Endothelialization by Topographical Features Is Mediated by PTP1B-Dependent Endothelial Adherens Junctions Remodeling.

ACS Biomater Sci Eng 2021 May 4. Epub 2021 May 4.

Mechanobiology Institute, National University of Singapore, 117411 Republic of Singapore.

Endothelial Cells (ECs) form cohesive cellular lining of the vasculature and play essential roles in both developmental processes and pathological conditions. Collective migration and proliferation of endothelial cells (ECs) are key processes underlying endothelialization of vessels as well as vascular graft, but the complex interplay of mechanical and biochemical signals regulating these processes are still not fully elucidated. While surface topography and biochemical modifications have been used to enhance endothelialization , thus far such single-modality modifications have met with limited success. Read More

View Article and Full-Text PDF

Quantitative standardization of resident mouse behavior for studies of aggression and social defeat.

Neuropsychopharmacology 2021 May 3. Epub 2021 May 3.

Neuroscience Program, Michigan State University, East Lansing, MI, USA.

Territorial reactive aggression in mice is used to study the biology of aggression-related behavior and is also a critical component of procedures used to study mood disorders, such as chronic social defeat stress. However, quantifying mouse aggression in a systematic, representative, and easily adoptable way that allows direct comparison between cohorts within or between studies remains a challenge. Here, we propose a structural equation modeling approach to quantify aggression observed during the resident-intruder procedure. Read More

View Article and Full-Text PDF

Severe COVID-19 in the intensive care unit: a case series.

J Med Case Rep 2021 May 3;15(1):259. Epub 2021 May 3.

Faculty of Medicine, Department of Anesthesiology and Intensive Care, Universitas Pelita Harapan, Jl. M. H. Thamrin Boulevard 1100, Lippo Village Tangerang, Tangerang, Banten, 15811, Indonesia.

Background: Coronavirus disease 2019 (COVID-19) was first identified in Indonesia in March 2020, and the number of infections has grown exponentially. The situation is at its worst, overwhelming intensive care unit (ICU) resources and capacity.

Case Presentation: This is a single-center observational case study of 21 confirmed COVID-19 patients admitted to the ICU from March 20, 2020, to April 31, 2020. Read More

View Article and Full-Text PDF

Review of binary, ternary and quaternary mixtures for induction and maintenance of opioid free anesthesia.

Farm Hosp 2021 Apr 21;45(3):135-141. Epub 2021 Apr 21.

MRI Department. Central Research Services, Universidad de Almería, Almería. Spain.

Objective: To describe and organize the current information available on  binary, ternary and/or quaternary mixtures used in opioid-free anesthesia (OFA), as well as their physicochemical stability, in order to  facilitate its correct administration, optimize its use, and prevent potential  effectiveness and safety issues.

Method: A systematic review of the literature on OFA was conducted in  PubMed/Medline, Trissel, Micromedex, Lexicomp,  ww.ahfsdruginformation. Read More

View Article and Full-Text PDF

Multiple stressors shape invertebrate assemblages and reduce their trophic niche: A case study in a regulated stream.

Sci Total Environ 2021 Jun 4;773:145061. Epub 2021 Feb 4.

Univ Lyon, Université Claude Bernard Lyon 1, CNRS, ENTPE, UMR 5023 LEHNA, F-69622 Villeurbanne, France.

Few studies have addressed how the diversity of basal resources change with stream regulation and the potential consequences on river biota. We sampled invertebrates above and below a series of dams, over two years, at both downwelling and upwelling zones. In each zone, we recorded the daily temperature and flow variations, estimated the algal development, measured the available resources, and analysed carbon and nitrogen stable isotope compositions of the invertebrate community. Read More

View Article and Full-Text PDF

How to stick the landing: kangaroo rats use their tails to reorient during evasive jumps away from predators.

Integr Comp Biol 2021 May 3. Epub 2021 May 3.

Department of Biology, University of Idaho, 875 Perimeter Drive, Moscow, ID 83844, USA.

Tails are widespread in the animal world and play important roles in locomotor tasks, such as propulsion, maneuvering, stability, and manipulation of objects. Kangaroo rats, bipedal hopping rodents, use their tail for balancing during hopping, but the role of their tail during the vertical evasive escape jumps they perform when attacked by predators has yet to be determined. Because we observed kangaroo rats swinging their tails around their bodies while airborne following escape jumps, we hypothesized that kangaroo rats use their tails to not only stabilize their bodies while airborne, but also to perform aerial re-orientations. Read More

View Article and Full-Text PDF

Pharmacokinetic comparisons of six steroid saponins in rat plasma following oral administration of crude and stir-fried Fructus Tribuli extracts by UHPLC-MS/MS.

Biomed Chromatogr 2021 May 3:e5151. Epub 2021 May 3.

Shandong University of Traditional Chinese Medicine, Jinan, China.

Modern pharmacological studies have shown that Fructus Tribuli can improve sexual function and treat cardiovascular diseases. In this study, we focused on comparing the pharmacokinetics of crude Fructus Tribuli (CFT) and stir-fried Fructus Tribuli (SFT) to further clarify the changes in chemical composition in vivo. The quantitation of six analytes was performed in a triple quadrupole mass spectrometer using the multiple reaction monitoring mode. Read More

View Article and Full-Text PDF

ESTIMATION OF BALANCE STATUS IN PATIENTS WITH HEMIPARESIS: AN ARTIFICIAL NEURAL NETWORK IMPLEMENTATION.

Top Stroke Rehabil 2021 May 3:1-7. Epub 2021 May 3.

Retired, Adana, Turkey.

Although Balance Evaluation Systems Test (BESTest) is an important balance assessment tool to differentiate balance deficits, it is time consuming and tiring for hemiparetic patients. Using artificial neural networks (ANNs) to estimate balance status can be a practical and useful tool for clinicians. The aim of this study was to compare manual BESTest results and ANNs predictive results and to determine the highest contributions of BESTest sections by using ANNs predictive results of BESTest sections. Read More

View Article and Full-Text PDF

Host Specialisation, Immune Cross-Reaction and the Composition of Communities of Co-circulating Borrelia Strains.

Bull Math Biol 2021 May 3;83(6):66. Epub 2021 May 3.

Department of Ecology, Evolution and Environmental Biology, Columbia University, New York, USA.

We use mathematical modelling to examine how microbial strain communities are structured by the host specialisation traits and antigenic relationships of their members. The model is quite general and broadly applicable, but we focus on Borrelia burgdorferi, the Lyme disease bacterium, transmitted by ticks to mice and birds. In this system, host specialisation driven by the evasion of innate immunity has been linked to multiple niche polymorphism, while antigenic differentiation driven by the evasion of adaptive immunity has been linked to negative frequency dependence. Read More

View Article and Full-Text PDF

Research Progress of Circular RNA in Gastrointestinal Tumors.

Front Oncol 2021 15;11:665246. Epub 2021 Apr 15.

Department of Thoracic and Cardiovascular Surgery, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, China.

circular RNA (circRNA) is a closed ring structure formed by cyclic covalent bonds connecting the 5'-end and 3'-end of pre-mRNA. circRNA is widely distributed in eukaryotic cells. Recent studies have shown that circRNA is involved in the pathogenesis and development of multiple types of diseases, including tumors. Read More

View Article and Full-Text PDF

The Diarylheptanoid Curcumin Induces MYC Inhibition and Cross-Links This Oncoprotein to the Coactivator TRRAP.

Front Oncol 2021 15;11:660481. Epub 2021 Apr 15.

Institute of Biochemistry and Center for Molecular Biosciences Innsbruck (CMBI), University of Innsbruck, Innsbruck, Austria.

The c-Myc protein (MYC) is a transcription factor with strong oncogenic potential controlling fundamental cellular processes. In most human tumors, MYC is overexpressed by enhanced transcriptional activation, gene amplification, chromosomal rearrangements, or increased protein stabilization. To pharmacologically suppress oncogenic MYC functions, multiple approaches have been applied either to inhibit transcriptional activation of the endogenous gene, or to interfere with biochemical functions of aberrantly activated MYC. Read More

View Article and Full-Text PDF

Dynamic analysis of the role of innate immunity in SEIS epidemic model.

Eur Phys J Plus 2021 23;136(4):439. Epub 2021 Apr 23.

Department of Mathematics, University of Delhi, Delhi, New Delhi 110007 India.

Consideration of every important aspect while modeling a disease makes the model more precise and the disease eradication strategy more powerful. In the present paper, we analyze the importance of innate immunity on SEIS modeling. We propose an SEIS model with Holling type II and type III functions representing innate immunity. Read More

View Article and Full-Text PDF

GC-MS/MS method for determination and pharmacokinetics of sclareol in rat plasma after intravenous administration.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Apr 20;1173:122703. Epub 2021 Apr 20.

School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, People's Republic of China. Electronic address:

Sclareol, mainly isolated from Salvia officinalis, has a variety of pharmacological effects. In this work, a sensitive and rapid gas chromatography-tandem mass spectrometry (GC-MS/MS) method was first developed and validated for the determination and pharmacokinetics of sclareol in rat plasma. The chromatographic seperation of biosamples was performed with an HP-5MS column. Read More

View Article and Full-Text PDF

Ring-opening of five-membered heterocycles conjugated 4-isopropylresorcinol scaffold-based benzamides as HSP90 inhibitors suppressing tumor growth in vitro and in vivo.

Eur J Med Chem 2021 Apr 6;219:113428. Epub 2021 Apr 6.

School of Pharmacy, College of Pharmacy, Taipei Medical University, 250 Wuxing Street, Taipei, 11031, Taiwan; Biomedical Commercialization Center, Taipei Medical University, Taipei, 11031, Taiwan; Ph.D. Program in Drug Discovery and Development Industry, College of Pharmacy, Taipei Medical University, Taipei, 11031, Taiwan. Electronic address:

A series of ring-opened dihydroxybenzamides have been designed and synthesized as heat shock protein 90 inhibitors. One of derivatives, compound 6b ((N-ethyl-2,4-dihydroxy-5-isopropyl-N-(pyridin-3-yl)benzamide)) demonstrated remarkable antiproliferative activity against in human KRAS mutant A549 and EGFR T790 M mutant H1975 lung cancer cell lines with GI values of 0.07 and 0. Read More

View Article and Full-Text PDF

Fabrication and characterization of the W/O/W multiple emulsion through oleogelation of oil.

Food Chem 2021 Apr 19;358:129856. Epub 2021 Apr 19.

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address:

W/O/W emulsions were easily prepared by oleogelation of the oil phase using rice bran wax (RBX) and their microstructure, stability, rheology and protection of proanthocyanidins and β-carotene were investigated. Formation of the W/O/W emulsion was confirmed using confocal laser scanning microscopy and staining of the inner aqueous phase by tartrazine. The average particle size and viscosity of the emulsion increased as the RBX concentration increased. Read More

View Article and Full-Text PDF

Interaction of acridinedione dye with a globular protein in the presence of site selective and site specific binding drugs: Photophysical techniques assisted by molecular docking methods.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Apr 20;258:119814. Epub 2021 Apr 20.

Department of Chemistry, D.G. Vaishnav College (Autonomous), 833, Gokul Bagh, E.V.R. Periyar Road, Arumbakkam, Chennai 600106, Tamil Nadu, India. Electronic address:

Photophysical investigations and molecular docking studies of photoinduced electron transfer (PET) based fluorophores of acridine family with a globular protein, Bovine Serum Albumin (BSA) bound to non-narcotic drugs like phenylbutazone (PB) and flufenamic acid (FA) were carried out in aqueous solution. PB and FA are site specific and site selective drugs, wherein PB predominantly binds at the site (I) whereas FA selectively orients towards site (II) of BSA. Acridinedione (AD) dyes, both resorcinol and dimedone based are hydrophobic in nature and exhibits a combination of both hydrophobic and hydrogen-bonding interactions that are based on the binding sites in BSA. Read More

View Article and Full-Text PDF

Negatively charged nanoparticles of multiple materials inhibit shear-induced platelet accumulation.

Nanomedicine 2021 Apr 28:102405. Epub 2021 Apr 28.

GW Woodruff School of Mechanical Engineering, Georgia, Institute of Technology, Atlanta, GA; Parker H Petit Institute for Bioengineering and Biosciences, Georgia, Institute of Technology, Atlanta, GA. Electronic address:

Platelet accumulation by VWF under high shear rates at the site of atherosclerotic plaque rupture leads to myocardial infarction and stroke. Current anti-platelet therapies remain ineffective for a large percentage of the population, while presenting significant risks for bleeding. We explore a novel way to inhibit arterial thrombus formation. Read More

View Article and Full-Text PDF

Multiple components-pharmacokinetic studies on ten bioactive constituents of Peiyuan Tongnao Capsule, using parallel reaction monitoring mode.

Biomed Chromatogr 2021 Apr 30:e5153. Epub 2021 Apr 30.

State Key Laboratory of Component-based Chinese Medicine, Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin, P. R. China.

Peiyuan Tongnao Capsule (PTC) plays an important role in clinical application due to its excellent curative efficacy in the treatment of ischemic stroke and chronic cerebral circulation insufficiency. To standardize and rationalize the clinical application of PTC, a rapid and sensitive method based on ultra-high performance liquid chromatography/quadrupole-Orbitrap mass spectrometry (UHPLC/Q-Orbitrap-MS) with parallel reaction monitoring mode (PRM) was developed and validated for the pharmacokinetic study. Ten bioactive compounds (aucubin, salidroside, echinacoside, paeoniflorin, verbascoside, liquiritin, 2, 3, 5, 4'-tetrahydroxy stilbene-2-O-β-D-glucoside, coumarin, glycyrrhizic acid, and emodin) were simultaneously determined in rat plasma. Read More

View Article and Full-Text PDF

Synthetic biology approaches in regulation of targeted gene expression.

Curr Opin Plant Biol 2021 Apr 26;63:102036. Epub 2021 Apr 26.

Department of Horticultural Science, North Carolina State University, Raleigh, NC 27607, USA. Electronic address:

Synthetic biology approaches are highly sought-after to facilitate the regulation of targeted gene expression in plants for functional genomics research and crop trait improvement. To date, synthetic regulation of gene expression predominantly focuses at the transcription level via engineering of synthetic promoters and transcription factors, while pioneering examples have started to emerge for synthetic regulation of gene expression at the levels of mRNA stability, translation, and protein degradation. This review discusses recent advances in plant synthetic biology for the regulation of gene expression at multiple levels, and highlights their future directions. Read More

View Article and Full-Text PDF