29 results match your criteria spt6 mutant

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A Collection of Pre-mRNA Splicing Mutants in .

G3 (Bethesda) 2020 06 1;10(6):1983-1996. Epub 2020 Jun 1.

Institute of Plant and Microbial Biology, Academia Sinica, 128, Sec. 2, Academia Rd., Nangang District, Taipei, 11529 Taiwan.

To investigate factors influencing pre-mRNA splicing in plants, we conducted a forward genetic screen using an alternatively-spliced reporter gene in This effort generated a collection of sixteen mutants impaired in various splicing-related proteins, many of which had not been recovered in any prior genetic screen or implicated in splicing in plants. The factors are predicted to act at different steps of the spliceosomal cycle, snRNP biogenesis pathway, transcription, and mRNA transport. We have described eleven of the mutants in recent publications. Read More

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A conserved genetic interaction between Spt6 and Set2 regulates H3K36 methylation.

Nucleic Acids Res 2019 05;47(8):3888-3903

Department of Genetics, Harvard Medical School, Boston, MA, USA 02115.

The transcription elongation factor Spt6 and the H3K36 methyltransferase Set2 are both required for H3K36 methylation and transcriptional fidelity in Saccharomyces cerevisiae. However, the nature of the requirement for Spt6 has remained elusive. By selecting for suppressors of a transcriptional defect in an spt6 mutant, we have isolated several highly clustered, dominant SET2 mutations (SET2sup mutations) in a region encoding a proposed autoinhibitory domain. Read More

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A Herpesviral Immediate Early Protein Promotes Transcription Elongation of Viral Transcripts.

mBio 2017 06 13;8(3). Epub 2017 Jun 13.

Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA

Herpes simplex virus 1 (HSV-1) genes are transcribed by cellular RNA polymerase II (RNA Pol II). While four viral immediate early proteins (ICP4, ICP0, ICP27, and ICP22) function in some capacity in viral transcription, the mechanism by which ICP22 functions remains unclear. We observed that the FACT complex (comprised of SSRP1 and Spt16) was relocalized in infected cells as a function of ICP22. Read More

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Bidirectional terminators in Saccharomyces cerevisiae prevent cryptic transcription from invading neighboring genes.

Nucleic Acids Res 2017 Jun;45(11):6417-6426

Institut de recherches cliniques de Montréal, Montréal, Québec H2W 1R7, Canada.

Transcription can be quite disruptive for chromatin so cells have evolved mechanisms to preserve chromatin integrity during transcription, thereby preventing the emergence of cryptic transcripts from spurious promoter sequences. How these transcripts are regulated and processed remains poorly characterized. Notably, very little is known about the termination of cryptic transcripts. Read More

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The Composition of the Arabidopsis RNA Polymerase II Transcript Elongation Complex Reveals the Interplay between Elongation and mRNA Processing Factors.

Plant Cell 2017 Apr 28;29(4):854-870. Epub 2017 Mar 28.

Department of Cell Biology and Plant Biochemistry, Biochemistry Center, University of Regensburg, D-93053 Regensburg, Germany

Transcript elongation factors (TEFs) are a heterogeneous group of proteins that control the efficiency of transcript elongation of subsets of genes by RNA polymerase II (RNAPII) in the chromatin context. Using reciprocal tagging in combination with affinity purification and mass spectrometry, we demonstrate that in , the TEFs SPT4/SPT5, SPT6, FACT, PAF1-C, and TFIIS copurified with each other and with elongating RNAPII, while P-TEFb was not among the interactors. Additionally, NAP1 histone chaperones, ATP-dependent chromatin remodeling factors, and some histone-modifying enzymes including Elongator were repeatedly found associated with TEFs. Read More

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Regulation of chaperone binding and nucleosome dynamics by key residues within the globular domain of histone H3.

Epigenetics Chromatin 2016 30;9:17. Epub 2016 Apr 30.

Shady Side Academy Senior School, 423 Fox Chapel Road, Pittsburgh, PA 15238 USA ; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260 USA.

Background: Nucleosomes have an important role in modulating access of DNA by regulatory factors. The role specific histone residues have in this process has been shown to be an important mechanism of transcription regulation. Previously, we identified eight amino acids in histones H3 and H4 that are required for nucleosome occupancy over highly transcribed regions of the genome. Read More

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Spt6 regulates intragenic and antisense transcription, nucleosome positioning, and histone modifications genome-wide in fission yeast.

Mol Cell Biol 2013 Dec 7;33(24):4779-92. Epub 2013 Oct 7.

Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA.

Spt6 is a highly conserved histone chaperone that interacts directly with both RNA polymerase II and histones to regulate gene expression. To gain a comprehensive understanding of the roles of Spt6, we performed genome-wide analyses of transcription, chromatin structure, and histone modifications in a Schizosaccharomyces pombe spt6 mutant. Our results demonstrate dramatic changes to transcription and chromatin structure in the mutant, including elevated antisense transcripts at >70% of all genes and general loss of the +1 nucleosome. Read More

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December 2013

The cellular protein SPT6 is required for efficient replication of human cytomegalovirus.

J Virol 2012 Feb 14;86(4):2011-20. Epub 2011 Dec 14.

Department of Microbiology, University of Minnesota, Minneapolis, Minnesota, USA.

The human cytomegalovirus tegument protein UL69 has been shown to be required for efficient viral replication at low multiplicities of infection. Several functions have been associated with UL69, including its ability to regulate cell cycle progression, translation, and the export of viral transcripts from the nucleus to the cytoplasm. However, it remains unclear which, if any, of these activities contribute to the phenotype observed with the UL69 deletion mutant. Read More

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February 2012

Spt6 is required for heterochromatic silencing in the fission yeast Schizosaccharomyces pombe.

Mol Cell Biol 2011 Oct 15;31(20):4193-204. Epub 2011 Aug 15.

Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.

Spt6 is a conserved factor, critically required for several transcription- and chromatin-related processes. We now show that Spt6 and its binding partner, Iws1, are required for heterochromatic silencing in Schizosaccharomyces pombe. Our studies demonstrate that Spt6 is required for silencing of all heterochromatic loci and that an spt6 mutant has an unusual combination of heterochromatic phenotypes compared to previously studied silencing mutants. Read More

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October 2011

High nitrogen insensitive 9 (HNI9)-mediated systemic repression of root NO3- uptake is associated with changes in histone methylation.

Proc Natl Acad Sci U S A 2011 Aug 25;108(32):13329-34. Epub 2011 Jul 25.

Biochimie et Physiologie Moléculaire des Plantes, Unité Mixte de Recherche 5004, Institut National de la Recherche Agronomique-Centre National de la Recherche Scientifique-Sup Agro-UM2, Institut de Biologie Intégrative des Plantes, F-34060 Montpellier, France.

In plants, root nitrate uptake systems are under systemic feedback repression by the N satiety of the whole organism, thus adjusting the N acquisition capacity to the N demand for growth; however, the underlying molecular mechanisms are largely unknown. We previously isolated the Arabidopsis high nitrogen-insensitive 9-1 (hni9-1) mutant, impaired in the systemic feedback repression of the root nitrate transporter NRT2.1 by high N supply. Read More

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Histone chaperone Spt6 is required for class switch recombination but not somatic hypermutation.

Proc Natl Acad Sci U S A 2011 May 25;108(19):7920-5. Epub 2011 Apr 25.

Department of Immunology and Genomic Medicine, Frontier Technology Center, Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.

Activation-induced cytidine deaminase (AID) is shown to be essential and sufficient to induce two genetic alterations in the Ig loci: class switch recombination (CSR) and somatic hypermutation (SHM). However, it is still unknown how a single-molecule AID differentially regulates CSR and SHM. Here we identified Spt6 as an AID-interacting protein by yeast two-hybrid screening and immunoprecipitation followed by mass spectrometry. Read More

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Control of chromatin structure by spt6: different consequences in coding and regulatory regions.

Mol Cell Biol 2011 Feb 22;31(3):531-41. Epub 2010 Nov 22.

Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.

Spt6 is a highly conserved factor required for normal transcription and chromatin structure. To gain new insights into the roles of Spt6, we measured nucleosome occupancy along Saccharomyces cerevisiae chromosome III in an spt6 mutant. We found that the level of nucleosomes is greatly reduced across some, but not all, coding regions in an spt6 mutant, with nucleosome loss preferentially occurring over highly transcribed genes. Read More

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February 2011

Deletion of Candida albicans SPT6 is not lethal but results in defective hyphal growth.

Fungal Genet Biol 2010 Apr 12;47(4):288-96. Epub 2010 Jan 12.

Department of Pediatrics, Women & Infants Hospital of Rhode Island, Warren Alpert Medical School of Brown University, Providence, RI, United States.

As a means to study surface proteins involved in the yeast to hypha transition, human monoclonal antibody fragments (single-chain variable fragments, scFv) have been generated that bind to antigens expressed on the surface of Candida albicans yeast and/or hyphae. A cDNA expression library was constructed from hyphae, and screened for immunoreactivity with scFv5 as a means to identify its cognate antigen. A reactive clone contained the 3' end of the C. Read More

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Cooperation between the INO80 complex and histone chaperones determines adaptation of stress gene transcription in the yeast Saccharomyces cerevisiae.

Mol Cell Biol 2009 Sep 20;29(18):4994-5007. Epub 2009 Jul 20.

Department of Biochemistry and Cell Biology, Max F Perutz Laboratories, University of Vienna, Vienna, Austria.

In yeast, environmental stresses provoke sudden and dramatic increases in gene expression at stress-inducible loci. Stress gene transcription is accompanied by the transient eviction of histones from the promoter and the transcribed regions of these genes. We found that mutants defective in subunits of the INO80 complex, as well as in several histone chaperone systems, exhibit extended expression windows that can be correlated with a distinct delay in histone redeposition during adaptation. Read More

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September 2009

Uncoupling of the patterns of chromatin association of different transcription elongation factors by a histone H3 mutant in Saccharomyces cerevisiae.

Eukaryot Cell 2009 Feb 1;8(2):257-60. Epub 2008 Dec 1.

Biology Department, Hendrix College, 1600 Washington Avenue, Conway, AR 72032, USA.

The transcription elongation complexes yFACT, Spt4/Spt5, and Spt6/Iws1 were previously shown to follow similar patterns of association across transcribed genes in Saccharomyces cerevisiae. Using a histone H3 mutant, we now provide evidence that the mechanism of association of yFACT across genes is separable from that adopted by Spt4/Spt5 and Spt6/Iws1. Read More

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February 2009

A genetic screen in Saccharomyces cerevisiae identifies new genes that interact with mex67-5, a temperature-sensitive allele of the gene encoding the mRNA export receptor.

Mol Genet Genomics 2009 Jan 26;281(1):125-34. Epub 2008 Nov 26.

Department of Biochemistry and Molecular Biology, Universitat de Valencia, c/Dr. Moliner, 50, Burjassot (Valencia), 46100, Spain.

The Mex67p protein, together with Mtr2p, functions as the mRNA export receptor in Saccharomyces cerevisiae by interacting with both mRNA and nuclear pore complexes. To identify genes that interact functionally with MEX67, we used transposon insertion to search for mutations that suppressed the temperature-sensitive mex67-5 allele. Four suppressors are described here. Read More

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January 2009

Requirements for chromatin reassembly during transcriptional downregulation of a heat shock gene in Saccharomyces cerevisiae.

FEBS J 2008 Jun 25;275(11):2956-64. Epub 2008 Apr 25.

Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology, University of Aarhus, Denmark.

Heat shock genes respond to moderate heat stress by a wave of transcription. The induction phase is accompanied by the massive eviction of histones, which later reassemble with DNA during the ensuing phase of transcription downregulation. In this article, we identify determinants of this reassembly throughout the heat shock protein 104 gene (HSP104) transcription unit. Read More

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Spn1 regulates the recruitment of Spt6 and the Swi/Snf complex during transcriptional activation by RNA polymerase II.

Mol Cell Biol 2008 Feb 17;28(4):1393-403. Epub 2007 Dec 17.

Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523, USA.

We investigated the timing of the recruitment of Spn1 and its partner, Spt6, to the CYC1 gene. Like TATA binding protein and RNA polymerase II (RNAPII), Spn1 is constitutively recruited to the CYC1 promoter, although levels of transcription from this gene, which is regulated postrecruitment of RNAPII, are low. In contrast, Spt6 appears only after growth in conditions in which the gene is highly transcribed. Read More

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February 2008

The role of the SPT6 chromatin remodeling factor in zebrafish embryogenesis.

Dev Biol 2007 Jul 3;307(2):214-26. Epub 2007 May 3.

Department of Neurobiology and Behavior, Stony Brook University, Stony Brook, New York 11794, USA.

Somitogenesis is a highly controlled process that results in segmentation of the paraxial mesoderm. Notch pathway activity in the presomitic mesoderm is fundamental for management of synchronized gene expression which is necessary for regulation of somitogenesis. We have isolated an embryonic lethal mutation, SBU2, that causes somite formation defects very similar to Notch pathway mutants. Read More

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The Spt6 SH2 domain binds Ser2-P RNAPII to direct Iws1-dependent mRNA splicing and export.

Genes Dev 2007 Jan;21(2):160-74

Regulatory Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA.

Spt6 promotes transcription elongation at many genes and functions as a histone H3 chaperone to alter chromatin structure during transcription. We show here that mammalian Spt6 binds Ser2-phosphorylated (Ser2P) RNA polymerase II (RNAPII) through a primitive SH2 domain, which recognizes phosphoserine rather than phosphotyrosine residues. Surprisingly, a point mutation in the Spt6 SH2 domain (R1358K) blocked binding to RNAPIIo without affecting transcription elongation rates in vitro. Read More

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January 2007

The Drosophila trithorax group protein Kismet facilitates an early step in transcriptional elongation by RNA Polymerase II.

Development 2005 Apr 23;132(7):1623-35. Epub 2005 Feb 23.

Department of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.

The Drosophila trithorax group gene kismet (kis) was identified in a screen for extragenic suppressors of Polycomb (Pc) and subsequently shown to play important roles in both segmentation and the determination of body segment identities. One of the two major proteins encoded by kis (KIS-L) is related to members of the SWI2/SNF2 and CHD families of ATP-dependent chromatin-remodeling factors. To clarify the role of KIS-L in gene expression, we examined its distribution on larval salivary gland polytene chromosomes. Read More

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Transcription elongation factors repress transcription initiation from cryptic sites.

Science 2003 Aug;301(5636):1096-9

Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.

Previous studies have suggested that transcription elongation results in changes in chromatin structure. Here we present studies of Saccharomyces cerevisiae Spt6, a conserved protein implicated in both transcription elongation and chromatin structure. Our results show that, surprisingly, an spt6 mutant permits aberrant transcription initiation from within coding regions. Read More

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Deletion of the CSB homolog, RAD26, yields Spt(-) strains with proficient transcription-coupled repair.

Nucleic Acids Res 2001 Jul;29(14):3080-6

Laboratory for Cancer Research, Rutgers, The State University of New Jersey, 164 Frelinghuysen Road, Piscataway, NJ 08854-8020, USA.

It has been previously shown that disruption of RAD26 in yeast strain W303-1B results in a strain that is deficient in transcription-coupled repair (TCR), the preferential repair of the transcribed strand of an expressed gene over the non-transcribed strand and the rest of the genome. RAD26 encodes a protein that is homologous to Cockayne syndrome group B protein (CSB) and is a member of the SWI2/SNF2 family of DNA-dependent ATPases involved in chromatin remodeling. Like the rad26 mutant, cells from Cockayne syndrome patients are defective in TCR. Read More

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Mutations in the SPT4, SPT5, and SPT6 genes alter transcription of a subset of histone genes in Saccharomyces cerevisiae.

Genetics 1996 Aug;143(4):1543-54

Program in Molecular Biology, Sloan Kettering Cancer Center, New York, New York 10021, USA.

The SPT4, SPT5, and SPT6 gene products define a class of transcriptional repressors in Saccharomyces cerevisiae that are thought to function through their effects on chromatin assembly or stability. Mutations in these genes confer a similar range of phenotypes to mutations in HIR genes, which encode transcriptional repressors that regulate expression of many of the core histone genes. Here we show that mutations in the three SPT genes also affect transcription of the histone genes that reside at the HTA1-HTB1 locus. Read More

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Faithful chromosome transmission requires Spt4p, a putative regulator of chromatin structure in Saccharomyces cerevisiae.

Mol Cell Biol 1996 Jun;16(6):2838-47

Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.

A chromosome transmission fidelity (ctf) mutant, s138, of Saccharomyces cerevisiae was identified by its centromere (CEN) transcriptional readthrough phenotype, suggesting perturbed kinetochore integrity in vivo. The gene complementing the s138 mutation was found to be identical to the S. cerevisiae SPT4 gene. Read More

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Molecular analysis of the SNF8 gene of Saccharomyces cerevisiae.

Yeast 1995 Mar;11(3):219-24

Department of Microbiology, Columbia University, New York, NY 10032, USA.

Mutations in the SNF8 gene impair derepression of the SUC2 gene, encoding invertase, in response to glucose limitation of Saccharomyces cerevisiae. We report here the cloning of the SNF8 gene by complementation. Sequence analysis predicts a 26,936-dalton product. Read More

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Functional interdependence of the yeast SNF2, SNF5, and SNF6 proteins in transcriptional activation.

Proc Natl Acad Sci U S A 1991 Apr;88(7):2687-91

Department of Genetics and Development, Columbia University College of Physicians and Surgeons, New York, NY 10032.

The SNF2, SNF5, and SNF6 genes of Saccharomyces cerevisiae are required for expression of a variety of differently regulated genes. Previous evidence implicated the SNF5 protein in transcriptional activation, and a DNA-bound LexA-SNF5 fusion protein was shown to activate expression of a nearby promoter. Here, we examine the functional relationship of the SNF2, SNF5, and SNF6 proteins. Read More

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SSN20 is an essential gene with mutant alleles that suppress defects in SUC2 transcription in Saccharomyces cerevisiae.

Mol Cell Biol 1987 Feb;7(2):672-8

Dominant and recessive mutations at the SSN20 locus were previously isolated as extragenic suppressors of mutations in three genes (SNF2, SNF5, and SNF6) that are required in trans to derepress invertase expression. All ssn20 alleles cause recessive, temperature-sensitive lethality. In this study we cloned the SSN20 gene, identified a 4. Read More

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February 1987

The SPT6 gene is essential for growth and is required for delta-mediated transcription in Saccharomyces cerevisiae.

Mol Cell Biol 1987 Feb;7(2):679-86

Mutations in the Saccharomyces cerevisiae SPT6 gene were originally identified as one class of extragenic suppressors of Ty and delta insertion mutations in the 5' noncoding regions of HIS4 and LYS2. We cloned SPT6 and constructed a null allele by gene disruption. Haploid spores carrying the spt6 null allele were inviable, indicating that the SPT6 gene is essential for mitotic growth. Read More

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February 1987
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