105,049 results match your criteria spontaneously

Membrane hyperpolarization abolishes calcium oscillations that prevent induced acrosomal exocytosis in human sperm.

FASEB J 2021 Jun;35(6):e21478

Instituto de Biología y Medicina Experimental (IBYME), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.

Sperm capacitation is essential to gain fertilizing capacity. During this process, a series of biochemical and physiological modifications occur that allow sperm to undergo acrosomal exocytosis (AE). At the molecular level, hyperpolarization of the sperm membrane potential (Em) takes place during capacitation. Read More

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Diabetic cataract in the Nile grass rat: A longitudinal phenotypic study of pathology formation.

FASEB J 2021 Jun;35(6):e21593

Molecular Biomarkers Nano-Imaging Laboratory, Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.

Diabetes is a major risk factor for cataract, the leading cause of blindness worldwide. There is an unmet need for a realistic model of diabetic cataract for mechanistic and longitudinal studies, as existing models do not reflect key aspects of the complex human disease. Here, we introduce and characterize diabetic cataract in the Nile grass rat (NGR, Arvicanthis niloticus), an established model of metabolic syndrome and type 2 diabetes (T2D). Read More

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A native conjugative plasmid confers potential selective advantages to plant growth-promoting Bacillus velezensis strain GH1-13.

Commun Biol 2021 May 14;4(1):582. Epub 2021 May 14.

Department of Microbiology, Daegu Catholic University School of Medicine, Daegu, Republic of Korea.

The conjugative plasmid (pBV71) possibly confers a selective advantage to Bacillus velezensis strain GH1-13, although a selective marker gene is yet to be identified. Here we show that few non-mucoid wild-type GH1-13 cells are spontaneously converted to mucoid variants with or without the loss of pBV71. Mucoid phenotypes, which contain or lack the plasmid, become sensitive to bacitracin, gramicidin, selenite, and tellurite. Read More

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Impact of prone position in non-intubated spontaneously breathing patients admitted to the ICU for severe acute respiratory failure due to COVID-19.

J Crit Care 2021 May 1;64:199-204. Epub 2021 May 1.

Service de Médecine intensive et réanimation, Hôpital Ambroise Paré, Assistance Publique Hôpitaux de Paris, France; INSERM UMR 1018, Clinical Epidemiology Team, CESP, Université de Paris Saclay, Villejuif, France. Electronic address:

Purpose: Studies performed in spontaneously breathing patients with mild to moderate respiratory failure suggested that prone position (PP) in COVID-19 could be beneficial.

Materials And Methods: Consecutive critically ill patients with COVID-19 were enrolled in four ICUs. PP sessions lasted at least 3 h each and were performed twice daily. Read More

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Validation of protein arginine methyltransferase 5 (PRMT5) as a candidate therapeutic target in the spontaneous canine model of non-Hodgkin lymphoma.

PLoS One 2021 14;16(5):e0250839. Epub 2021 May 14.

Division of Hematology, Department of Internal Medicine, The Ohio State University, Columbus, Ohio, United States of America.

Non-Hodgkin lymphoma (NHL) is a heterogeneous group of blood cancers arising in lymphoid tissues that commonly effects both humans and dogs. Protein arginine methyltransferase 5 (PRMT5), an enzyme that catalyzes the symmetric di-methylation of arginine residues, is frequently overexpressed and dysregulated in both human solid and hematologic malignancies. In human lymphoma, PRMT5 is a known driver of malignant transformation and oncogenesis, however, the expression and role of PRMT5 in canine lymphoma has not been explored. Read More

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Unwanted mainstream nitritation-denitritation causing massive NO emissions in a continuous activated sludge process.

Water Sci Technol 2021 May;83(9):2207-2217

Helsinki Region Environmental Services Authority Helsinki and Aalto University: Espoo, Wastewater Treatment, P.O. Box 320, FI-00066 HSY, Finland E-mail:

Nitrous oxide emissions can contribute significantly to the carbon footprint of municipal wastewater treatment plants even though emissions from conventional nitrogen removal processes are assumed to be moderate. An increased risk for high emissions can occur in connection with process disturbances and nitrite (NO) accumulation. This work describes the findings at a large municipal wastewater treatment plant where the levels of NO in the activated sludge process effluent were spontaneously and strongly increased on several activated sludge lines which was suspected to be due to shortcut nitrogen removal that stabilized for several months. Read More

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Supercontinuum generation in AsS waveguides fabricated without direct etching.

Opt Lett 2021 May;46(10):2413-2416

We report a supercontinuum generation (SCG) in a waveguide that spontaneously forms without an etching process during the deposition of a core material on a preformed ${\rm{Si}}{{\rm{O}}_2}$ substructure. The mechanism of dispersion control for this new, to the best of our knowledge, type of waveguide is analyzed by numerical simulation, which results in a design rule to achieve a target dispersion profile by adjusting the substructure geometry. SCG is experimentally demonstrated with a waveguide made of ${\rm{A}}{{\rm{s}}_2}{{\rm{S}}_3}$, chalcogenide glass, which has low material absorption over the mid-IR range. Read More

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Entanglement and Purification Transitions in Non-Hermitian Quantum Mechanics.

Phys Rev Lett 2021 Apr;126(17):170503

Joint Center for Quantum Information and Computer Science, NIST/University of Maryland, College Park, Maryland 20742 USA.

A quantum system subject to continuous measurement and postselection evolves according to a non-Hermitian Hamiltonian. We show that, as one increases the strength of postselection, this non-Hermitian Hamiltonian can undergo a spectral phase transition. On one side of this phase transition (for weak postselection), an initially mixed density matrix remains mixed at all times, and an initially unentangled state develops volume-law entanglement; on the other side, an arbitrary initial state approaches a unique pure state with low entanglement. Read More

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Role of endoscopic ultrasonography in the management of peripancreatic collections. Diagnostic and therapeutic approach.

Minerva Gastroenterol (Torino) 2021 May 14. Epub 2021 May 14.

Gastroenterology and Digestive Endoscopy Unit, Forlì-Cesena Hospitals, AUSL Romagna, Forlì-Cesena, Italy.

Pancreatic fluid collections are surrounded by a wall of granulation tissue and may contain necrotic debris. They occur following a severe acute pancreatitis and most of the cases resolve spontaneously after several weeks. However, their management may lead to a very hardto-treat condition, requiring a multidisciplinary approach. Read More

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Efficacy and Safety of Mirodenafil Oro-Dispersible Film in Korean Patients with Erectile Dysfunction: A Double-Blind, Randomized, Placebo-Controlled, Parallel-Group, Multicenter, Phase IV Study.

World J Mens Health 2021 Jan 21. Epub 2021 Jan 21.

Department of Urology, Konkuk University Chungju Hospital, Konkuk University School of Medicine, Chungju, Korea.

Purpose: To investigate the efficacy, safety, and tolerability of oro-dispersible film (ODF) formulation of mirodenafil 50 mg and 100 mg for the treatment of patients with erectile dysfunction (ED) in Korea.

Materials And Methods: A multicenter, randomized, double-blind, placebo-controlled, parallel-group study of 129 subjects was performed. Subjects were randomized to either placebo or mirodenafil ODF 50 mg or 100 mg to be taken in an "on demand" manner for 8 weeks. Read More

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January 2021

Cardiac, biochemical, and histopathological analysis reveals impaired heart function in hypertensive rats with apical periodontitis.

Int Endod J 2021 May 13. Epub 2021 May 13.

Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.

Aim: To investigate the association between experimentally induced apical periodontitis (AP) and heart function in hypertensive rats.

Methodology: Forty-eight normotensive Wistar albino and spontaneously hypertensive rats were divided into four equal groups: control (C), normotensive with AP (AP), spontaneously hypertensive (SHR), and SHR with AP (SHR+AP). AP was induced on the first right mandibular molars by exposing the pulp chambers to the oral environment for four weeks and confirmed radiographically. Read More

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Intrapartum antibiotic prophylaxis for women who are screened positive for group B streptococcal colonisation: Clinical compliance with the guideline.

Aust N Z J Obstet Gynaecol 2021 May 13. Epub 2021 May 13.

Hunter New England Local Health District, Nurses and Midwives Research Centre, The University of Newcastle, Callaghan, New South Wales, Australia.

Background: In high income countries, ~30% of pregnant women are provided with intrapartum antibiotic prophylaxis (IAP) for early-onset group B streptococcal infection (EOGBSI). The infection rate is low, 0.2/1000 livebirths in our jurisdiction, and others. Read More

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Influence of Swimming Program on the Blood Pressure of Pregnant Hypertensive Rats and Their Fetuses.

Reprod Sci 2021 May 13. Epub 2021 May 13.

Laboratory of System Physiology and Reproductive Toxicology, Institute of Biological and Health Sciences, Federal University of Mato Grosso (UFMT), Barra do Garças, Mato Grosso State, Brazil.

The hypertension incidence and its complication on pregnant women are growing and can lead to adverse consequences on their fetuses. However, it is known that regular exercise practice can be healthful to hypertensive pregnant women but harmful to fetal growth. So, the objective of this study was to evaluate the effects of exercise beginning before pregnancy or during pregnancy on the maternal blood pressure and reproductive outcome and on the fetal development of spontaneously hypertensive rats (SHR). Read More

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Apelin receptor upregulation in spontaneously hypertensive rat contributes to the enhanced vascular smooth muscle cell proliferation by activating autophagy.

Ann Transl Med 2021 Apr;9(8):627

Department of Geriatric Medicine, The First Affiliated Hospital, Nanjing Medical University, Nanjing, China.

Background: Proliferation of vascular smooth muscle cells (VSMCs) plays a vital role in the progression of vascular remodeling and hypertension. Apelin-13 promotes VSMC proliferation of normal rats. This study was designed to investigate the roles of apelin receptor (APJ) and apelin-13 in VSMC proliferation of hypertension rats and underlying mechanisms. Read More

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Fugitive aerosols in the intensive care unit: a narrative review.

Ann Transl Med 2021 Apr;9(7):592

Aerogen, IDA Business Park, Dangan, Galway, Ireland.

The risk of unintended inhalation of fugitive aerosols is becoming a topic of increasing interest in the healthcare arena. These fugitive aerosols may be bioaerosols, generated by the patient themselves through cough or sneeze, or they may be therapeutic medical aerosols, generated by therapeutic medical aerosol generators with the intent of delivery to a specific patient's respiratory tract. This review focus' on therapeutic aerosols in the intensive care unit (ICU) only, those typically generated by nebulisers. Read More

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Aerosol delivery via invasive ventilation: a narrative review.

Ann Transl Med 2021 Apr;9(7):588

Department of Respiratory Care, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

In comparison with spontaneously breathing non-intubated subjects, intubated, mechanically ventilated patients encounter various challenges, barriers, and opportunities in receiving medical aerosols. Since the introduction of mechanical ventilation as a part of modern critical care medicine during the middle of the last century, aerosolized drug delivery by jet nebulizers has become a common practice. However, early evidence suggested that aerosol generators differed in their efficacies, and the introduction of newer aerosol technology (metered dose inhalers, ultrasonic nebulizer, vibrating mesh nebulizers, and soft moist inhaler) into the ventilator circuit opened up the possibility of optimizing inhaled aerosol delivery during mechanical ventilation that could meet or exceed the delivery of the same aerosols in spontaneously breathing patients. Read More

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A specific low-density neutrophil population correlates with hypercoagulation and disease severity in hospitalized COVID-19 patients.

JCI Insight 2021 May 10;6(9). Epub 2021 May 10.

Department of Microbiology and Immunology.

SARS coronavirus 2 (SARS-CoV-2) is a novel viral pathogen that causes a clinical disease called coronavirus disease 2019 (COVID-19). Although most COVID-19 cases are asymptomatic or involve mild upper respiratory tract symptoms, a significant number of patients develop severe or critical disease. Patients with severe COVID-19 commonly present with viral pneumonia that may progress to life-threatening acute respiratory distress syndrome (ARDS). Read More

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Common clonal origin of chronic myelomonocytic leukemia and B cell acute lymphoblastic leukemia in a patient with a germline variant.

Cold Spring Harb Mol Case Stud 2021 May 13. Epub 2021 May 13.

McGill University.

Hematological malignancies are broadly divided into myeloid and lymphoid neoplasms, reflecting the two major cellular lineages of the hematopoietic system. It is generally rare for hematological malignancies to spontaneously progress with a switch from myeloid to lymphoid lineage. We describe the exceptional case of a patient who sequentially developed myelodysplastic syndrome (MDS), chronic myelomonocytic leukemia (CMML), and B cell acute lymphoblastic leukemia (B-ALL), as well as our investigation into the underlying pathogenesis. Read More

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Sleep-Related Rhythmic Movement Disorder.

Sleep Med Clin 2021 Jun 15;16(2):315-321. Epub 2021 Apr 15.

European Sleep Instituto, Panama, Panama.

Sleep-related rhythmic movements disorder (SRRMD), typically considered a benign pediatric sleep disorder, comprise a group of movement disorders that occur predominantly early in childhood with an average age of onset of 9 months of age. Although it usually resolves spontaneously as the child ages, it can persist into adulthood. In this article, the authors review the identification, diagnosis, and management of SRRMD in children and adults. Read More

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Chemotherapy-based gonadotoxicity risk evaluation as a predictor of reproductive outcomes in post-pubertal patients following ovarian tissue cryopreservation.

BMC Womens Health 2021 May 13;21(1):201. Epub 2021 May 13.

Department of Obstetrics and Gynecology, Hadassah Ein-Kerem Medical Center and Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel.

Background: The sterilizing effect of cancer treatment depends mostly on the chemotherapy regimen and extent of radiotherapy. Prediction of long-term reproductive outcomes among cancer survivors according to chemo-radiotherapy regimen may improve pre-treatment fertility preservation counseling and future reproductive outcomes.

Methods: The aim of this study was to evaluate long term reproductive outcomes in cancer survivors according to gonadotoxicity risk estimation of the chemo-radiotherapy regimens utilized. Read More

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Cesk Slov Oftalmol 2021 ;77(2):88-93

Purpose: The aim is to present a report of a case of bilateral eye injury with bilateral blowout fracture caused by a high-pressure water jet from a fire hose in a 16-year-old girl during a competition for young firefighters.

Methods: We present a case report of a 16-year-old female patient with bilateral extensive eyelid contusion, oedema and lacerations of upper eyelids, lacerations of bulbar conjunctiva, contusion of both eyes, left optic nerve and bilateral blowout fractures of orbits.  Results: The initial ocular examination revealed "hand motion" in the right eye and no light perception in the left eye. Read More

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January 2021

Effect of disorder on superconductivity of NbN thin films studied using x-ray absorption spectroscopy.

J Phys Condens Matter 2021 May 13. Epub 2021 May 13.

UGC-DAE Consortium for Scientific Research - Indore, Khandwa Road, Indore, Indore, 452001, INDIA.

The superconducting transition temperature (Tc) of rock-salt type niobium nitride (\dNbN) typically varies between 9 to 17 K and the theoretically predicted value of 18 K has not been achieved hitherto. The low Tc in \dNbN has been assigned to some structural disorder which is always present irrespective of the microstructure (polycrystalline or epitaxial), methods or conditions adopted during the growth of NbN thin films. In this work, we investigate the atomic origin of such suppression of the Tc in \dNbN thin films by employing combined methods of experiments and ab-initio simulations. Read More

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Application of the SMALP technology to the isolation of GPCRs from low-yielding cell lines.

Biochim Biophys Acta Biomembr 2021 May 10:183641. Epub 2021 May 10.

Department of Biopharmacy, Medical University of Lublin, ulica W. Chodźki 4a, Lublin, Poland. Electronic address:

The ability of styrene-maleic acid (SMAc) co-polymers to spontaneously insert into biological membranes can be exploited to extract G protein-coupled receptors (GPCRs) embedded in styrene-maleic acid lipid particles (SMALPs), preserving the native environment around the protein and thus enhancing the feasibility of functional studies. So far, the SMALP technology has been primarily employed on non-mammalian cells and protocols are not optimized for adherent human cell lines, which cannot be harvested in large amounts. In this work, a fine investigation of key parameters affecting the formation of SMALPs was undertaken with the purpose of maximizing the yield of extraction of a recombinant form of human β-adrenergic receptor (rhβAR) from HEK293T cells. Read More

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Cryptic surface-associated multicellularity emerges through cell adhesion and its regulation.

PLoS Biol 2021 May 13;19(5):e3001250. Epub 2021 May 13.

Department of Evolutionary Biology and Environmental Studies, University of Zürich, Zürich, Switzerland.

The repeated evolution of multicellularity leads to a wide diversity of organisms, many of which are sessile, including land plants, many fungi, and colonial animals. Sessile organisms adhere to a surface for most of their lives, where they grow and compete for space. Despite the prevalence of surface-associated multicellularity, little is known about its evolutionary origin. Read More

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"Web-based, second-best togetherness": Psychosocial group intervention with children of Holocaust survivors during COVID-19.

Irit V Felsen

Am J Orthopsychiatry 2021 ;91(2):171-180

Department of Psychology, Columbia University.

Evidence from multiple samples of trauma-exposed populations across the globe suggests that intergenerational trauma constitutes a biopsychological risk factor which manifests itself throughout the life cycle of offspring of trauma survivors. Prior empirical studies have shown that adult children of Holocaust survivors (OHS, also referred to in select quotes as 2G for "Second Generation") are vulnerable to life-threatening situations. This study aimed to examine the reactions of OHS to the COVID-19 pandemic, which posed a serious threat to the lives of many, in particular to older adults. Read More

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January 2021

Acoustic Droplet-Assisted Superhydrophilic-Superhydrophobic Microarray Platform for High-Throughput Screening of Patient-Derived Tumor Spheroids.

ACS Appl Mater Interfaces 2021 May 13. Epub 2021 May 13.

Key Laboratory of Artificial Micro/Nano-Structures, Ministry of Education School of Physics and Technology, Wuhan University, Wuhan 430072, China.

Cell-based high-throughput screening is a key step in the current disease-based research, drug development, and precision medicine. However, it is challenging to establish a rapid culture and screening platform for rare cells (patient-derived) due to the obvious differences between the traditional 2D cell model and the tumor microenvironment, as well as the lack of a low-consumption screening platform for low numbers of cells. Here, we developed an acoustic drop-assisted superhydrophilic-superhydrophobic microarray platform for the rapid culture and screening of a few cells. Read More

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Hyperglycaemia-Induced Contractile Dysfunction and Apoptosis in Cardiomyocyte-Like Pulsatile Cells Derived from Mouse Embryonic Stem Cells.

Cardiovasc Toxicol 2021 May 13. Epub 2021 May 13.

Department of Human Biology, Faculty of Health Sciences, University of Cape Town, Observatory, Cape Town, 7925, South Africa.

Hyperglycaemia, a key metabolic abnormality in diabetes mellitus, is implicated in pathological cardiogenesis during embryological development. However, the underlying mechanisms and potential therapeutic targets remain unknown. We, therefore, studied the effect of hyperglycaemia on mouse embryonic stem cell (mESC) cardiac differentiation. Read More

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The abnormalities of adrenomedullary hormonal system in genetic hypertension: Their contribution to altered regulation of blood pressure.

Physiol Res 2021 May 12. Epub 2021 May 12.

Laboratory of Experimental Hypertension, Institute of Physiology of the Czech Academy of Sciences, Prague 4, Czech Republic.

It is widely accepted that sympathetic nervous system plays a crucial role in the development of hypertension. On the other hand, the role of adrenal medulla (the adrenomedullary component of the sympathoadrenal system) in the development and maintenance of high blood pressure in man as well as in experimental models of hypertension is still controversial. Spontaneously hypertensive rats (SHR) are the most widely used animal model of human essential hypertension characterized by sympathetic hyperactivity. Read More

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Acute intranasal dopamine application counteracts the reversal learning deficit of spontaneously hypertensive rats in an attentional set-shifting task.

Psychopharmacology (Berl) 2021 May 12. Epub 2021 May 12.

M et P Pharma AG Emmetten Switzerland and Oceanographic Center, Nova Southeastern University, Fort Lauderdale, FL, USA.

Rationale: Studies on the attention-deficit/hyperactivity disorder (ADHD) have concluded that the disorder might be caused by a deficit in the inhibitory control of executive functions because of dopamine hypofunction. Recently, the intranasal route has emerged as an effective alternative means for sending dopamine directly to the brain. However, whether the treatment can ameliorate the deficits of inhibitory control in ADHD remains unknown. Read More

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Sorption isotherms and thermodynamic properties of the dry silage of red tilapia viscera ( spp.) obtained in a direct solar dryer.

Heliyon 2021 Apr 18;7(4):e06798. Epub 2021 Apr 18.

Nutrition and Food Technology Group, University of Antioquia, 67 Street Nº 53 - 108, 050010 Medellín, Colombia.

The sorption isotherms, thermodynamic properties and calculation for confirming the isokinetic theory of dry chemical silage of red tilapia viscera ( spp.) obtained in a direct passive solar dryer were determined. Sorption isotherms were carried out at 15, 25, 35 and 45 °C using a static gravimetric method. Read More

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