13,889 results match your criteria spontaneous calcium


Amitriptyline Decreases GABAergic Transmission in Basal Forebrain Neurons Using an Optogenetic Model of Aging.

Front Aging Neurosci 2021 28;13:673155. Epub 2021 May 28.

Department of Neuroscience and Experimental Therapeutics, College of Medicine, Texas A&M University Health Science Center, Bryan, TX, United States.

The antidepressant drug amitriptyline is used in the treatment of clinical depression and a variety of neurological conditions such as anxiety, neuropathic pain disorders and migraine. Antidepressants are associated with both therapeutic and untoward effects, and their use in the elderly has tripled since the mid-1990s. Because of this widespread use, we are interested in testing the acute effects of amitriptyline on synaptic transmission at therapeutic concentrations well below those that block voltage-gated calcium channels. Read More

View Article and Full-Text PDF

Efficacy of RyR2 inhibitor EL20 in induced pluripotent stem cell-derived cardiomyocytes from a patient with catecholaminergic polymorphic ventricular tachycardia.

J Cell Mol Med 2021 Jun 10. Epub 2021 Jun 10.

Department of Molecular Physiology & Biophysics, Cardiovascular Research Institute, Baylor College of Medicine, Houston, TX, USA.

Catecholaminergic polymorphic ventricular tachycardia (CPVT) is an inherited cardiac arrhythmia syndrome that often leads to sudden cardiac death. The most common form of CPVT is caused by autosomal-dominant variants in the cardiac ryanodine receptor type-2 (RYR2) gene. Mutations in RYR2 promote calcium (Ca ) leak from the sarcoplasmic reticulum (SR), triggering lethal arrhythmias. Read More

View Article and Full-Text PDF

X-linked hypophosphatemia and burosumab: practical clinical points from the French experience.

Joint Bone Spine 2021 Jun 5:105208. Epub 2021 Jun 5.

APHP, Reference Center for Rare Diseases of Calcium and Phosphate Metabolism, and Filière OSCAR, Paris, France; APHP, Endocrinology and Diabetes for Children, Bicêtre Paris-Sud Hospital, Le Kremlin-Bicêtre, France. Electronic address:

Hereditary hypophosphatemia with increased FGF23 levels are rare inherited metabolic diseases characterized by low serum phosphate because of impaired renal tubular phosphate reabsorption. The most common form is X-linked hypophosphatemia (XLH), secondary to a mutation in the PHEX gene. In children, XLH is often manifested by rickets, delayed development of gait, lower limb deformities, growth retardation, craniosynostosis, and spontaneous dental abscesses. Read More

View Article and Full-Text PDF

Reduced Expression of GABA Receptor Alpha2 Subunit Is Associated With Disinhibition of DYT-THAP1 Dystonia Patient-Derived Striatal Medium Spiny Neurons.

Front Cell Dev Biol 2021 21;9:650586. Epub 2021 May 21.

Department of Neurology, Hannover Medical School, Hanover, Germany.

DYT-THAP1 dystonia (formerly DYT6) is an adolescent-onset dystonia characterized by involuntary muscle contractions usually involving the upper body. It is caused by mutations in the gene encoding for the transcription factor Thanatos-associated protein (THAP) domain containing apoptosis-associated protein 1 and inherited in an autosomal-dominant manner with reduced penetrance. Alterations in the development of striatal neuronal projections and synaptic function are known from transgenic mice models. Read More

View Article and Full-Text PDF

Coexistence of idiopathic intracranial hypertension and optic disc drusen in a latin american patient: case report.

Arch Soc Esp Oftalmol (Engl Ed) 2021 Jun 30;96(6):326-331. Epub 2020 Aug 30.

Departamento de Salud Pública, Universidad de Caldas, Manizales, Colombia.

Optic nerve drusen are acellular concretions of calcium located in the parenchyma of the optical nerve head described as the most common cause of pseudo-papilloedema, which makes it difficult to differentiate from a true optical disc oedema. Despite it being rare, the drusen of the optic nerve and the papilloedema secondary to idiopathic intracranial hypertension can coexist in the same patient. The case is presented of a 34 year-old woman referred to the Ophthalmology Department with visual discomfort, headaches, and pulsatile tinnitus of two months onset. Read More

View Article and Full-Text PDF

Efficient absorptive removal of Cd(Ⅱ) in aqueous solution by biochar derived from sewage sludge and calcium sulfate.

Bioresour Technol 2021 May 27;336:125333. Epub 2021 May 27.

Office of Teaching Affairs, Guilin University of Technology, Guilin 541004, China.

Biochar derived from co-pyrolysis of sewage sludge and calcium sulfate was used to remove Cd(II) from aqueous solution. The results showed that the Cd(Ⅱ) adsorption better followed Freundlich model, and the maximum adsorption capacities were 109.0 mg/g (288 K), 127. Read More

View Article and Full-Text PDF

Characterization of Cecal Smooth Muscle Contraction in Laying Hens.

Vet Sci 2021 May 26;8(6). Epub 2021 May 26.

Institute of Animal Science, University of Hohenheim, 70593 Stuttgart, Germany.

The ceca play an important role in the physiology of the gastrointestinal tract in chickens. Nevertheless, there is a gap of knowledge regarding the functionality of the ceca in poultry, especially with respect to physiological cecal smooth muscle contraction. The aim of the current study is the ex vivo characterization of cecal smooth muscle contraction in laying hens. Read More

View Article and Full-Text PDF

Successful Postnatal Cardiopulmonary Resuscitation Due to Defibrillation.

Children (Basel) 2021 May 20;8(5). Epub 2021 May 20.

Division of Neonatology, Department of Pediatrics and Adolescent Medicine, Medical University of Graz, 8036 Graz, Austria.

An asphyxiated term neonate required postnatal resuscitation. After six minutes of cardio-pulmonary resuscitation (CPR) and two doses of epinephrine, spontaneous circulation returned, but was shortly followed by ventricular fibrillation. CPR and administration of magnesium, calcium gluconate, and sodium bicarbonate did not improve the neonate's condition. Read More

View Article and Full-Text PDF

Transient incubation of cultured hippocampal neurons in the absence of magnesium induces rhythmic and synchronized epileptiform-like activity.

Sci Rep 2021 May 31;11(1):11374. Epub 2021 May 31.

CNC-Center for Neuroscience and Cell Biology, Faculty of Medicine, University of Coimbra, Rua Larga, 3004-504, Coimbra, Portugal.

Cell culture models are important tools to study epileptogenesis mechanisms. The aim of this work was to characterize the spontaneous and synchronized rhythmic activity developed by cultured hippocampal neurons after transient incubation in zero Mg to model Status Epilepticus. Cultured hippocampal neurons were transiently incubated with a Mg-free solution and the activity of neuronal networks was evaluated using single cell calcium imaging and whole-cell current clamp recordings. Read More

View Article and Full-Text PDF

Errors of Commission in Cardiac Arrest Care in the Intensive Care Unit.

J Intensive Care Med 2021 Jul;36(7):749-757

Department of Pediatrics, Critical Care Medicine, 22916Dartmouth Hitchcock Medical Center, Lebanon, NH, USA.

Introduction: Cardiopulmonary arrests (CPAs) are common in the intensive care unit (ICU). However, effects of protocol deviations on CPA outcomes in the ICU are relatively unknown.

Objectives: To establish the frequency of errors of commission (EOCs) during CPAs in the ICU and their relationship with CPA outcomes. Read More

View Article and Full-Text PDF

Inactivation of Presenilin in inhibitory neurons results in decreased GABAergic responses and enhanced synaptic plasticity.

Mol Brain 2021 May 25;14(1):85. Epub 2021 May 25.

Department of Neurology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, USA.

Mutations in the Presenilin genes are the major genetic cause of Alzheimer's disease (AD). Presenilin (PS) is highly expressed in the hippocampus, which is particularly vulnerable in AD. Previous studies of PS function in the hippocampus, however, focused exclusively on excitatory neurons. Read More

View Article and Full-Text PDF

Reported Benefits of Low-Dose Naltrexone Appear to Be Independent of the Endogenous Opioid System Involving Proopiomelanocortin Neurons and Beta-Endorphin.

eNeuro 2021 May 24. Epub 2021 May 24.

Department of Biomedical Sciences and Program in Molecular, Cellular, and Integrative Neurosciences, Colorado State University

Naltrexone is an opioid receptor antagonist approved for the treatment of alcohol and opioid use disorders at doses of 50-150 mg/d. Naltrexone has also been prescribed at much lower doses (3-6 mg/d) for the off-label treatment of inflammation and pain. Currently, a compelling mechanistic explanation for the reported efficacy of low-dose naltrexone (LDN) is lacking and none of the proposed mechanisms can explain patient reports of improved mood and sense of well-being. Read More

View Article and Full-Text PDF

Homeostatic Depression Shows Heightened Sensitivity to Synaptic Calcium.

Front Cell Neurosci 2021 7;15:618393. Epub 2021 May 7.

Department of Anatomy and Cell Biology, University of Iowa Carver College of Medicine, Iowa City, IA, United States.

Synapses and circuits rely on homeostatic forms of regulation in order to transmit meaningful information. The neuromuscular junction (NMJ) is a well-studied synapse that shows robust homeostatic control of function. Most prior studies of homeostatic plasticity at the NMJ have centered on presynaptic homeostatic potentiation (PHP). Read More

View Article and Full-Text PDF

Primary hyperparathyroidism presenting as a brown tumor with hypercalcemia crisis in a second-trimester pregnant woman: A case report.

Authors:
Yun Xu Yingying Yu

Medicine (Baltimore) 2021 May;100(20):e25968

The Second Affiliated Hospital Zhejiang University School of Medicine, Hangzhou, China.

Introduction: Primary hyperparathyroidism (PHPT) in pregnancy is rare and unrecognized because the maternal physiological adaptations blurs the symptoms. There is no standard treatment strategy for maternal PHPT. Early diagnosis and interventions can prevent catastrophic consequences to the mother and fetus. Read More

View Article and Full-Text PDF

Transcriptome-based screening of ion channels and transporters in a migratory chondroprogenitor cell line isolated from late-stage osteoarthritic cartilage.

J Cell Physiol 2021 May 18. Epub 2021 May 18.

Department of Regenerative Medicine, State Research Institute Centre for Innovative Medicine, Vilnius, Lithuania.

Chondrogenic progenitor cells (CPCs) may be used as an alternative source of cells with potentially superior chondrogenic potential compared to mesenchymal stem cells (MSCs), and could be exploited for future regenerative therapies targeting articular cartilage in degenerative diseases such as osteoarthritis (OA). In this study, we hypothesised that CPCs derived from OA cartilage may be characterised by a distinct channelome. First, a global transcriptomic analysis using Affymetrix microarrays was performed. Read More

View Article and Full-Text PDF

Effects of phosphodiesterase-1 inhibitor on pulmonary vein electrophysiology and arrhythmogenesis.

Eur J Clin Invest 2021 May 18:e13585. Epub 2021 May 18.

Taipei Heart Institute, Taipei Medical University, Taipei, Taiwan.

Introduction: Phosphodiesterase (PDE) isoform inhibitors have mechanical and electrical effects on the heart. Inhibition of PDE-1 enzymes is a novel strategy for treating heart failure. However, the electrophysiological effects of PDE-1 inhibition on the heart remain unclear. Read More

View Article and Full-Text PDF

Cortical propagation tracks functional recovery after stroke.

PLoS Comput Biol 2021 May 17;17(5):e1008963. Epub 2021 May 17.

Institute for Complex Systems (ISC), National Research Council (CNR), Sesto Fiorentino, Italy.

Stroke is a debilitating condition affecting millions of people worldwide. The development of improved rehabilitation therapies rests on finding biomarkers suitable for tracking functional damage and recovery. To achieve this goal, we perform a spatiotemporal analysis of cortical activity obtained by wide-field calcium images in mice before and after stroke. Read More

View Article and Full-Text PDF

Three-Dimensional Model of Sub-Plasmalemmal Ca Microdomains Evoked by the Interplay Between ORAI1 and InsP Receptors.

Front Immunol 2021 28;12:659790. Epub 2021 Apr 28.

Unit of Theoretical Chronobiology, Faculté des Sciences CP231, Université Libre de Bruxelles (ULB), Brussels, Belgium.

Ca signaling plays an essential role in T cell activation, which is a key step to start an adaptive immune response. During the transition from a quiescent to a fully activated state, Ca microdomains characterized by reduced spatial and temporal extents are observed in the junctions between the plasma membrane (PM) and the endoplasmic reticulum (ER). Such Ca responses can also occur in response to T cell adhesion to other cells or extracellular matrix proteins in otherwise unstimulated T cells. Read More

View Article and Full-Text PDF

[Nutritional status of elderly hemodialysis people in Tunisia].

Nephrol Ther 2021 Jun 11;17(3):168-174. Epub 2021 May 11.

Service A, institut national de nutrition et de technologie alimentaire de Tunis, 8, rue des Lilas, cité Jardins de l'Aouina, 2045 Tunis, Tunisie.

Introduction: The nutritional status of old hemodialysis patients determines their prognosis. The aim of this study was to evaluate the spontaneous dietary intake of hemodialysis of elderly patients.

Methods: This cross-sectional descriptive study included 40 elderly hemodialysis patients recruited at the M8 nephrology department of Charles Nicolle hospital in Tunis. Read More

View Article and Full-Text PDF

Osteitis Fibrosa Cystica and pathological fractures-the classic but neglected skeletal manifestation of primary hyperparathyroidism: a case report.

BMC Musculoskelet Disord 2021 May 14;22(1):443. Epub 2021 May 14.

Division of Endocrinology and Metabolism, Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.

Background: Osteitis fibrosa cystica is the classic manifestation of primary hyperparathyroidism (PHPT), occurs after prolonged exposure of bone to high serum parathyroid hormone (PTH) level. It has become increasingly rare due to early detection of PHPT.

Case Presentation: A 37-year-old woman was referred to our institution for fixation of multiple fractures of upper and lower extremities that had been reoccurring in the past 5 years. Read More

View Article and Full-Text PDF

The bone is the major source of high circulating intact fibroblast growth factor-23 in acute murine polymicrobial sepsis induced by cecum ligation puncture.

PLoS One 2021 14;16(5):e0251317. Epub 2021 May 14.

Department of Biomedical Sciences, University of Veterinary Medicine Vienna, Vienna, Austria.

Fibroblast growth factor-23 (FGF23), a bone-produced hormone, plays a critical role in mineral homeostasis. Human diseases associated with excessive intact circulating FGF23 (iFGF23) result in hypophosphatemia and low vitamin D hormone in patients with normal kidney function. In addition, there is accumulating evidence linking FGF23 with inflammation. Read More

View Article and Full-Text PDF

Inhibition of human sperm motility and capacitation by ziram is mediated by decreasing tyrosine protein kinase.

Ecotoxicol Environ Saf 2021 May 10;218:112281. Epub 2021 May 10.

Department of Obstetrics and Gynecology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, 109 Xueyuan West Road, Wenzhou, Zhejiang 325027, China. Electronic address:

Many endocrine disruptors may interfere with sperm motility, hyperactivation, and capacitation, thereby leading to male infertility. In the current study, we screened 14 endocrine disruptors, including plant ingredients, cigarette ingredients, minerals, insecticides and fungicides, plastics, and plasticizers, to inhibit human sperm motility and forward motility. Only ziram, a dithiocarbamate fungicide, can effectively inhibit sperm motility, forward motility, hyperactivation, capacitation, and spontaneous acrosome reaction of normal human spermatozoa. Read More

View Article and Full-Text PDF

Dopamine transporter is a master regulator of dopaminergic neural network connectivity.

J Neurosci 2021 May 10. Epub 2021 May 10.

Department of Neuroscience, University of Florida, Gainesville, FL, USA

Dopaminergic neurons of the substantia nigra (SNC) and ventral tegmental area (VTA) exhibit spontaneous firing activity. The dopaminergic neurons in these regions have been shown to exhibit differential sensitivity to neuronal loss and psychostimulants targeting dopamine transporter. However, it remains unclear whether these regional differences scale beyond individual neuronal activity to regional neuronal networks. Read More

View Article and Full-Text PDF

Differential proteomic analysis of tibial subchondral bone from male and female guinea pigs with spontaneous osteoarthritis.

Exp Ther Med 2021 Jun 15;21(6):633. Epub 2021 Apr 15.

Department of Spine Surgery, Beijing Jishuitan Hospital, Beijing 100035, P.R. China.

A proteomic study on the tibial subchondral bone in guinea pigs with spontaneous osteoarthritis was performed to investigate the molecular alterations that occur in early osteoarthritis. A total of 132 healthy Hartley guinea pigs (aged 1 month; 66 female and 66 male) were randomly divided into 11 groups of six. Changes in articular cartilage and tibial subchondral bone were assessed using macroscopic examinations and micro-computed tomography. Read More

View Article and Full-Text PDF

Neural Systems Under Change of Scale.

Front Comput Neurosci 2021 21;15:643148. Epub 2021 Apr 21.

Department of Neuroimaging, King's College London, London, United Kingdom.

We derive a theoretical construct that allows for the characterisation of both scalable and scale free systems within the dynamic causal modelling (DCM) framework. We define a dynamical system to be "scalable" if the same equation of motion continues to apply as the system changes in size. As an example of such a system, we simulate planetary orbits varying in size and show that our proposed methodology can be used to recover Kepler's third law from the timeseries. Read More

View Article and Full-Text PDF

Amelioration of diabetic retinopathy in db/db mice by treatment with different proportional three active ingredients from Tibetan medicine Berberis dictyophylla F.

J Ethnopharmacol 2021 Aug 6;276:114190. Epub 2021 May 6.

Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China. Electronic address:

Ethnopharmacological Relevance: Berberis dictyophylla F., a famous Tibetan medicine, has been used to prevent and treat diabetic retinopathy (DR) for thousands of years in clinic. However, its underlying mechanisms remain unclear. Read More

View Article and Full-Text PDF

Functional and Structural Properties of Highly Responsive Somatosensory Neurons in Mouse Barrel Cortex.

Cereb Cortex 2021 May 8. Epub 2021 May 8.

IZKF Aachen, Medical School, RWTH Aachen University, 52074 Aachen, Germany.

Sparse population activity is a well-known feature of supragranular sensory neurons in neocortex. The mechanisms underlying sparseness are not well understood because a direct link between the neurons activated in vivo, and their cellular properties investigated in vitro has been missing. We used two-photon calcium imaging to identify a subset of neurons in layer L2/3 (L2/3) of mouse primary somatosensory cortex that are highly active following principal whisker vibrotactile stimulation. Read More

View Article and Full-Text PDF

Molecular mechanism of apelin-13 regulation of colonic motility in rats.

Eur J Pharmacol 2021 Aug 5;904:174149. Epub 2021 May 5.

Department of Gastroenterology, Renmin Hospital of Wuhan University, 430060, Wuhan, Hubei Province, China. Electronic address:

Apelin is a novel neuropeptide identified as the endogenous ligand for the apelin receptor. Apelin and its receptor are widely distributed in the gastrointestinal tract. Studies have reported that apelin-13 is involved in modulating gastrointestinal motility; however, the evidence is insufficient and the relevant mechanism is still not fully clear. Read More

View Article and Full-Text PDF

Calcium-permeable AMPA receptor activity and GluA1 trafficking in the basolateral amygdala regulate operant alcohol self-administration.

Addict Biol 2021 May 5:e13049. Epub 2021 May 5.

Bowles Center for Alcohol Studies, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.

Addiction is viewed as maladaptive glutamate-mediated neuroplasticity that is regulated, in part, by calcium-permeable AMPA receptor (CP-AMPAR) activity. However, the contribution of CP-AMPARs to alcohol-seeking behavior remains to be elucidated. We evaluated CP-AMPAR activity in the basolateral amygdala (BLA) as a potential target of alcohol that also regulates alcohol self-administration in C57BL/6J mice. Read More

View Article and Full-Text PDF

Telomerase/myocardin expressing mesenchymal cells induce survival and cardiovascular markers in cardiac stromal cells undergoing ischaemia/reperfusion.

J Cell Mol Med 2021 Jun 5;25(12):5381-5390. Epub 2021 May 5.

Department of Pathology, Cardiology Division, University of Pisa, Pisa, Italy.

Cardiac stromal cells (CSCs) contain a pool of cells with supportive and paracrine functions. Various types of mesenchymal stromal cells (MSCs) can influence CSCs in the cardiac niche through their paracrine activity. Ischaemia/reperfusion (I/R) leads to cell death and reduction of the paracrine activity of CSCs. Read More

View Article and Full-Text PDF