10,835 results match your criteria spiked human


Novel API Coated Catheter Removes Amyloid-β from Plasma of Patients with Alzheimer's Disease.

HSOA J Alzheimers Neurodegener Dis 2021 6;7(1). Epub 2021 Oct 6.

Recombinant Technologies LLC, 1090 Meriden Waterbury Turnpike, Suite 1, Cheshire, Connecticut 06410, USA.

Alzheimer's disease (AD) is the most common cause of dementia, characterized by the deposition of Amyloid-beta (Aβ) plaques in the brain. We have previously developed Amytrap peptide (the active pharmacological ingredient, API) and linked it to a sepharose bead matrix by click chemistry to form Amytrapper matrix, which was able to bind and remove Aβ from human sera and plasma spiked with biotinylated Aβ42 (bio-Aβ42) . To extend the logic of the previous studies, the current study investigates whether the Amytrap peptide coated inside a medically viable polycarbonate catheter (Amytrapper catheter) could bind and retain Aβ from the human sera. Read More

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October 2021

Development of a Fast Liquid Chromatography Coupled to Mass Spectrometry Method (LC-MS/MS) to Determine Fourteen Lipophilic Shellfish Toxins Based on Fused-Core Technology: In-House Validation.

Mar Drugs 2021 Oct 24;19(11). Epub 2021 Oct 24.

Centro de Investigacións Mariñas (CIMA), Pedras de Corón s/n, 36620 Vilanova de Arousa, Spain.

Prevalence and incidence of the marine toxins (paralytic, amnesic, and lipophilic toxins) including the so-called emerging toxins (these are, gymnodimines, pinnatoxins, or spirolides among others) have increased in recent years all over the world. Climate change, which is affecting the distribution of their producing phytoplankton species, is probably one of the main causes. Early detection of the toxins present in a particular area, and linking the toxins to their causative phytoplankton species are key tools to minimize the risk they pose for human consumers. Read More

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October 2021

Rapid detection of Pseudomonas aeruginosa based on lab-on-a-chip platform using immunomagnetic separation, light scattering, and machine learning.

Anal Chim Acta 2022 Jan 29;1189:339223. Epub 2021 Oct 29.

Postdoctoral Innovation Practice, Shenzhen Polytechnic, Liuxian Avenue, No. 7098, Nanshan District, Shenzhen, 518055, Guangdong Province, China. Electronic address:

The rapid detection of the pathogenic bacteria in patient samples is crucial to expedient patient care. The proposed approach reports the development of a novel lab-on-a-chip device for the rapid detection of P. aeruginosa based on immunomagnetic separation, optical scattering, and machine learning. Read More

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January 2022

Quantitative detection of 6-thioguanine in body fluids based on a free-standing liquid membrane SERS substrate.

Anal Bioanal Chem 2021 Nov 23. Epub 2021 Nov 23.

Faculty of Geosciences and Environmental Engineering, Southwest Jiaotong University, Chengdu, 611756, Sichuan, China.

The adverse reactions caused by 6-thioguanine (6-TG) in anti-cancer treatment are closely related to the dose, leading to the urgent need for clinical monitoring of its concentration. In this work, a highly reproducible free-standing liquid membrane (FLM) surface-enhanced Raman spectroscopy (SERS) substrate was developed to detect 6-TG in human urine and serum quantitatively. Briefly, a prepared sample was adjusted to pH 2 and mixed with concentrated core-shell bimetallic nanoparticle (AgAu NP) suspension. Read More

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November 2021

[Determination of bongkrekic acid in tremella and auricularia auricular by improved QuEChERS method combined with ultra-high performance liquid chromatography-triple quadrupole mass spectrometry].

Se Pu 2021 Dec;39(12):1368-1373

An improved QuEChERS (quick, easy, cheap, effective, rugged, safe) method, combined with ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), was used to determine bongkrekic acid (BA) in tremella and auricularia auricular. BA is a fat-soluble, fatal bacterial toxin produced by the aerobic gram-negative bacteria Burkholderia gladioli pathovar cocovenenans. Tremella and auricularia auricular, which have rich nutritional values, are traditional edible fungi in China that are very popular among Chinese consumers. Read More

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December 2021

[Simultaneous determination of eight carbamate pesticide residues in tomato, rice, and cabbage by online solid phase extraction/purification-high performance liquid chromatography-tandem mass spectrometry].

Se Pu 2021 Dec;39(12):1324-1330

National Institutes for Food and Drug Control, Beijing 100050, China.

Carbamate pesticides are a class of synthetic pesticides having wide antimicrobial spectrum, good insecticidal efficacy, and a short residual period. These pesticides are used in agriculture, forestry, and animal husbandry. Their widespread use in the last two decades has led to the existence of drug residues in the environment, which are transferred to food, thereby raising concerns regarding the potential threat to human health. Read More

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December 2021

[Simultaneous determination of three allergic proteins in rice and products by high performance liquid chromatography-tandem mass spectrometry combined with stable isotope-labeled peptides].

Se Pu 2021 Dec;39(12):1314-1323

Rice Product Quality Inspection and Supervision Center, Ministry of Agriculture and Rural Affairs, China National Rice Research Institute, Hangzhou 310006, China.

Rice is an important cereal that is consumed as both an energy and protein source by a large proportion of the population worldwide. However, clinical studies have found that rice grains are responsible for cases of severe asthma, eczema, and atopic dermatitis in some adult patients. Several allergenic proteins have been identified and biochemically and immunochemically characterized from rice grains. Read More

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December 2021

Unknown cold-tolerant Clostridium spp.: Characteristics and potential to cause meat spoilage.

Food Microbiol 2022 Apr 27;102:103916. Epub 2021 Sep 27.

Chair of Food Safety, Faculty of Veterinary Medicine, LMU Munich, Schönleutnerstr. 8, 85764, Oberschleißheim, Germany.

Clostridium spp. are ubiquitous bacteria and often found in foods and animals. Some species are pathogenic, others food spoiling or commensals. Read More

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Salmonella Enteritidis survival in different temperatures and nutrient solution pH levels in hydroponically grown lettuce.

Food Microbiol 2022 Apr 11;102:103898. Epub 2021 Sep 11.

Department of Agricultural Sciences, Biotechnology and Food Science, Cyprus University of Technology, Limassol, Cyprus. Electronic address:

Due to climate change, with contaminated and less fertile soils, and intense weather phenomena, a turn towards hydroponic vegetable production has been made. Hydroponic cultivation of vegetables is considered to be a clean, safe and environmentally friendly growing technique; however, incidence of microbial contamination i.e. Read More

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A novel spectrofluorimetric method for determination of Lomefloxacin adopting on Zinc (II) chelation strategy: Application in human plasma.

Luminescence 2021 Nov 21. Epub 2021 Nov 21.

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Fayoum University, Fayoum, Egypt.

A new sensitive and instantaneous spectrofluorimetric method for efficient determination of lomefloxacin (LMX) in its pure, dosage form and human plasma was designed. The developed method depends on formation of a metal-chelation compound of LMX as a ligand with Zn (II) in a buffer of acetate (pH 5.5). Read More

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November 2021

Towards Real-Time and Affordable Strain-Level Metagenomics-Based Foodborne Outbreak Investigations Using Oxford Nanopore Sequencing Technologies.

Front Microbiol 2021 5;12:738284. Epub 2021 Nov 5.

Transversal Activities in Applied Genomics, Sciensano, Brussels, Belgium.

The current routine laboratory practices to investigate food samples in case of foodborne outbreaks still rely on attempts to isolate the pathogen in order to characterize it. We present in this study a proof of concept using Shiga toxin-producing spiked food samples for a strain-level metagenomics foodborne outbreak investigation method using the MinION and Flongle flow cells from Oxford Nanopore Technologies, and we compared this to Illumina short-read-based metagenomics. After 12 h of MinION sequencing, strain-level characterization could be achieved, linking the food containing a pathogen to the related human isolate of the affected patient, by means of a single-nucleotide polymorphism (SNP)-based phylogeny. Read More

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November 2021

Rapid and selective detection of Bacillus cereus in food using cDNA-based up-conversion fluorescence spectrum copy and aptamer modified magnetic separation.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Nov 13:120618. Epub 2021 Nov 13.

School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China; College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, PR China. Electronic address:

A sensitive luminescent bioassay for the detection of Bacillus cereus (B. cereus), a common bacterium, harmful to human health, was established based on up-conversion fluorescence and magnetic separation technology. Herein, aptamers (Apt) are modified on the surface of magnetic nanoparticles (MNPs) to form Apt-MNPs capture probes. Read More

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November 2021

Influence of refrigeration and freezing in Microcystins and Cylindrospermopsin concentrations on fish muscle of tilapia (Oreochromis niloticus) and tench (Tinca tinca).

Food Chem Toxicol 2021 Nov 18:112673. Epub 2021 Nov 18.

Area of Toxicology, Faculty of Pharmacy, Universidad de Sevilla, Profesor García González Nº2, Spain.

The consumption of fish contaminated with cyanotoxins is an important public health issue due to their potential adverse effects. The aim of this study was to assess the influence of refrigeration (4 °C) and freezing (-20 °C) on the concentration of Cylindrospermopsin (CYN), Microcystins (MCs) and their combination in tilapia (Oreochromis niloticus) and tench (Tinca tinca). Fish muscle were spiked with a stock solution of each toxin to reach 750 μg/g dry weight (d. Read More

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November 2021

Label-free detection of SARS-CoV-2 Spike S1 antigen triggered by electroactive gold nanoparticles on antibody coated fluorine-doped tin oxide (FTO) electrode.

Anal Chim Acta 2021 Dec 21;1188:339207. Epub 2021 Oct 21.

DBT-National Institute of Animal Biotechnology (NIAB), Hyderabad, 500032, Telangana, India. Electronic address:

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, also known as 2019-nCov or COVID-19) outbreak has become a huge public health issue due to its rapid transmission making it a global pandemic. Here, we report fabricated fluorine doped tin oxide (FTO) electrodes/gold nanoparticles (AuNPs) complex coupled with in-house developed SARS-CoV-2 spike S1 antibody (SARS-CoV-2 Ab) to measure the response with Cyclic Voltammetry (CV) and Differential Pulse Voltammetry (DPV). The biophysical characterisation of FTO/AuNPs/SARS-CoV-2Ab was done via UV-Visible spectroscopy, Dynamic Light Scattering (DLS), and Fourier Transform Infrared Spectroscopy (FT-IR). Read More

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December 2021

Sandwich-type electrochemical aptasensor based on hollow mesoporous carbon spheres loaded with porous dendritic [email protected] nanoparticles as signal amplifier for ultrasensitive detection of cardiac troponin I.

Anal Chim Acta 2021 Dec 22;1188:339202. Epub 2021 Oct 22.

Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republic of China; Research Center of Analysis and Test, East China University of Science and Technology, Shanghai, 200237, People's Republic of China. Electronic address:

Signal amplification is crucial to improve the sensitivity for the electrochemical detection of cardiac troponin I (cTnI), one of the ideal biomarkers for early acute myocardial infarction (AMI) diagnosis. Herein, we developed a novel signal amplification strategy to construct a sandwich-type electrochemical aptasensor for the detection of cTnI. Core-shell [email protected] dendritic bimetallic nanoparticles loaded on melamine modified hollow mesoporous carbon spheres ([email protected] DNs/NH-HMCS) was prepared as labels to conjugate with thiol-modification DNA aptamers probe for signal amplification. Read More

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December 2021

Regenerative Flexible Upconversion-Luminescence Biosensor for Visual Detection of Diethylstilbestrol Based on Smartphone Imaging.

Anal Chem 2021 Nov 17. Epub 2021 Nov 17.

School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, P. R. China.

Diethylstilbestrol (DES), an endocrine disrupting chemical, has been linked to serious health problems in humans. In this work, a regenerative flexible upconversion-fluorescence biosensor was designed for the detection of DES in foodstuffs and environmental samples. Herein, amino-functionalized upconversion nanoparticles (UCNPs) were synthesized and immobilized on the surface of a flexible polydimethylsiloxane substrate, which was further modified with complementary DNA and dabcyl-labeled DES aptamer. Read More

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November 2021

Uptake of perfluoroalkyl substances, pharmaceuticals, and parabens by oyster mushrooms (Pleurotus ostreatus) and exposure risk in human consumption.

Chemosphere 2021 Nov 12:132898. Epub 2021 Nov 12.

Department of Biosystems and Technology, Swedish University of Agricultural Sciences, Växtskyddsvägen 3, SE-230 53, Alnarp, Sweden.

Organic micropollutants (MPs) pose potential threats to environmental ecosystems and human health. This study investigated uptake of perfluoroalkyl substances (PFASs), pharmaceuticals, and paraben by edible oyster mushrooms (Pleurotus ostreatus), cultivated on spiked growth substrate. Concentrations of pharmaceuticals and paraben in substrate showed a decreasing trend over a 20-day harvesting period, whereas PFAS concentrations were variable over the harvesting period. Read More

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November 2021

The use of laser-based diagnostics for the rapid identification of blood borne viruses in human plasma samples.

J Appl Microbiol 2021 Nov 14. Epub 2021 Nov 14.

Food and Drug Administration, Center for Biologics Evaluation and Research, Silver Spring, Maryland, USA.

Aims: To demonstrate the use of a laser-based method of detection as a potential diagnostic test for the rapid identification of blood borne viruses in human plasma.

Methods And Results: In this study, using light emissions from laser sparks on plasma samples, the successful differentiation of both human immunodeficiency virus (HIV) and hepatitis C virus (HCV) in both residual de-identified plasma samples and plasma samples spiked to clinically relevant levels with each virus were demonstrated using plasma from more than 20 individuals spanning six different blood types (O+, O-, A+, A-, B+, B-).

Conclusions: These experiments demonstrate that mathematical analysis of spectral data from laser sparks can provide accurate results within minutes. Read More

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November 2021

Altered vitamin K biodistribution and metabolism in experimental and human chronic kidney disease.

Kidney Int 2021 Nov 11. Epub 2021 Nov 11.

Department of Nephrology and Clinical Immunology, RWTH Aachen University Hospital, Aachen, Germany.

Chronic kidney disease (CKD) is accompanied with extensive cardiovascular calcification, in part correlating with functional vitamin K deficiency. Here, we sought to determine causes for vitamin K deficiency beyond reduced dietary intake. Initially, vitamin K uptake and distribution into circulating lipoproteins after a single administration of vitamin K1 plus K2 (menaquinone 4 and menaquinone 7, respectively) was determined in patients on dialysis therapy and healthy individuals. Read More

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November 2021

Micronutritional supplementation with a holoBLG based FSMP (food for specific medical purposes)-lozenge alleviates allergic symptoms in BALB/c mice: Imitating the protective farm effect.

Clin Exp Allergy 2021 Nov 13. Epub 2021 Nov 13.

Comparative Medicine, The Interuniversity Messerli Research Institute of the University of Veterinary Medicine Vienna, Medical University Vienna and University of Vienna, Vienna, Austria.

Background: Previously, the protective farm effect was imitated using the whey protein beta-lactoglobulin (BLG) that was spiked with iron-flavonoid complexes. Here, we formulated for clinical translation a lozenge as food for specific medical purposes (FSMP) using catechin-iron complexes as ligands for BLG. The lozenge was tested in vitro and in a therapeutical BALB/c mice model. Read More

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November 2021

Polyvinyl Alcohol Polymer Functionalized Graphene Oxide Decorated with Gadolinium Oxide for Sequestration of Radionuclides from Aqueous Medium: Characterization, Mechanism, and Environmental Feasibility Studies.

Polymers (Basel) 2021 Nov 6;13(21). Epub 2021 Nov 6.

Department of Environmental Engineering, Kwangwoon University, Seoul 01897, Korea.

Uranium (U(VI)) and thorium (Th(IV)) ions produced by the nuclear and mining industries cause water pollution, thereby harming the environment and human health. In this study, gadolinium oxide-decorated polyvinyl alcohol-graphene oxide composite (PGO-Gd) was developed using a simple hydrothermal process to treat U(VI) and Th(IV) ions in water. The developed material was structurally characterized by highly advanced spectroscopy and microscopy techniques. Read More

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November 2021

Lensless imaging-based discrimination between tumour cells and blood cells towards circulating tumour cell cultivation.

Analyst 2021 Nov 22;146(23):7327-7335. Epub 2021 Nov 22.

Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo, 184-8588, Japan.

Circulating tumour cells (CTCs) are recognized as important markers for cancer research. Nonetheless, the extreme rarity of CTCs in blood samples limits their availability for multiple characterization. The cultivation of CTCs is still technically challenging due to the lack of information of CTC proliferation, and it is difficult for conventional microscopy to monitor CTC cultivation owing to low throughput. Read More

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November 2021

Optimization of an ammonia assay based on transmembrane pH-gradient polymersomes.

Sci Rep 2021 Nov 11;11(1):22032. Epub 2021 Nov 11.

Department of Chemistry and Applied Biosciences, Institute of Pharmaceutical Sciences, ETH Zurich, Vladimir-Prelog-Weg 3, 8093, Zurich, Switzerland.

Reliable ammonia quantification assays are essential for monitoring ammonemia in patients with liver diseases. In this study, we describe the development process of a microplate-based assay for accurate, precise, and robust ammonia quantification in biological fluids, following regulatory guidelines on bioanalytical method validation. The assay is based on transmembrane pH-gradient polymersomes that encapsulate a pH-sensitive ratiometric fluorophore, the fluorescence signal of which correlates with the ammonia concentration in the sample. Read More

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November 2021

Perfluoroalkyl and polyfluoroalkyl substances (PFASs): An optimized LC-MS/MS procedure for feed analysis.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Oct 30;1186:123009. Epub 2021 Oct 30.

Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche "Togo Rosati", Via Cupa di Posatora 3, Ancona 60131, Italy. Electronic address:

Perfluoroalkyl and polyfluoroalkyl substances (PFASs) are extremely stable highly fluorinated aliphatic compounds, widely used in chemical and industrial applications since 1950s. They are ubiquitously detected in the environment and diet is the main route for human exposure. To ensure human safety, it is necessary to follow the whole food production chain, including animal feed. Read More

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October 2021

Mapping antibody binding using multiplexed epitope substitution analysis.

J Immunol Methods 2021 12 30;499:113178. Epub 2021 Oct 30.

Department of Chemical Engineering, University of California, Santa Barbara, CA 93106, USA.

A more complete understanding of antibody epitopes would aid the development of diagnostics, therapeutic antibodies, and vaccines. However, current methods for mapping antibody binding to epitopes require a targeted experimental approach, which limits throughput. To address these limitations, we developed Multiplexed Epitope Substitution Analysis (MESA) which can rapidly characterize various distinct epitopes using millions of antibody-binding peptides. Read More

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December 2021

Species-specific quantification of circulating ebolavirus burden using VP40-derived peptide variants.

PLoS Pathog 2021 11 8;17(11):e1010039. Epub 2021 Nov 8.

Center for Cellular and Molecular Diagnostics, Department of Biochemistry and Molecular Biology, School of Medicine, Tulane University, New Orleans, Louisiana, United States of America.

Six ebolavirus species are reported to date, including human pathogens Bundibugyo virus (BDBV), Ebola virus (EBOV), Sudan virus (SUDV), and Taï Forest virus (TAFV); non-human pathogen Reston virus (RESTV); and the plausible Bombali virus (BOMV). Since there are differences in the disease severity caused by different species, species identification and viral burden quantification are critical for treating infected patients timely and effectively. Here we developed an immunoprecipitation-coupled mass spectrometry (IP-MS) assay for VP40 antigen detection and quantification. Read More

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November 2021

Rapid and Sensitive Detection of Using CRISPR/Cas12a Combined With a Recombinase-Aided Amplification Assay.

Front Microbiol 2021 21;12:767315. Epub 2021 Oct 21.

Wenzhou Key Laboratory of Sanitary Microbiology, Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, China.

is an important zoonotic and aquatic pathogen and can cause vibriosis in humans and aquatic animals (especially farmed fish and shrimp species). Rapid and sensitive detection methods for are still required to diagnose human vibriosis early and reduce aquaculture losses. Herein, we developed a rapid and sensitive diagnostic method comprising a recombinase-aided amplification (RAA) assay and the CRISPR/Cas12a system (named RAA-CRISPR/Cas12a) to detect . Read More

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October 2021

The Compound ATH434 Prevents Alpha-Synuclein Toxicity in a Murine Model of Multiple System Atrophy.

J Parkinsons Dis 2021 Oct 30. Epub 2021 Oct 30.

Florey Institute of Neuroscience and Mental Health and the University of Melbourne, Parkville, Victoria, Australia.

Background: An elevation in iron levels, together with an accumulation of α-synuclein within the oligodendrocytes, are features of the rare atypical parkinsonian disorder, Multiple System Atrophy (MSA). We have previously tested the novel compound ATH434 (formally called PBT434) in preclinical models of Parkinson's disease and shown that it is brain-penetrant, reduces iron accumulation and iron mediated redox activity, provides neuroprotection, inhibits alpha synuclein aggregation and lowers the tissue levels of alpha synuclein. The compound was also well-tolerated in a first-in-human oral dosing study in healthy and older volunteers with a favorable, dose-dependent pharmacokinetic profile. Read More

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October 2021

Green sample pre-treatment technique coupled with UHPLC-MS/MS for the rapid biomonitoring of dietary poly-unsaturated (omega) fatty acids to predict health risks.

Chemosphere 2021 Oct 28:132685. Epub 2021 Oct 28.

Department of Neurology, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung City, 833, Taiwan. Electronic address:

Polyunsaturated fatty acids (PUFAs) consumption indicates beneficial effects on cardiovascular disease (CVD) and physiological processes in humans. However, the inappropriate ratio of omega-(ω)-PUFA levels in human blood is considered as raising the risk of CVD. Therefore, monitoring dietary ω-FAs in human serum is vital for early diagnosis for individuals to predict CVD risk. Read More

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October 2021

Validating and optimizing the method for molecular detection and quantification of SARS-CoV-2 in wastewater.

Sci Total Environ 2021 Nov 4:151434. Epub 2021 Nov 4.

Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada; Public Health Laboratories (ProvLab), Alberta Precision Laboratories (APL), Edmonton, Alberta, Canada. Electronic address:

Wastewater surveillance of SARS-CoV-2 has become a promising tool to estimate population-level changes in community infections and the prevalence of COVID-19 disease. Although many studies have reported the detection and quantification of SARS-CoV-2 in wastewater, remarkable variation remains in the methodology. In this study, we validated a molecular testing method by concentrating viruses from wastewater using ultrafiltration and detecting SARS-CoV-2 using one-step RT-qPCR assay. Read More

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November 2021