41,001 results match your criteria sphingolipids

Deepening of lipidome annotation by associating cross-metathesis reaction with mass spectrometry: application to an in vitro model of corneal toxicity.

Anal Bioanal Chem 2021 Jun 14. Epub 2021 Jun 14.

C-TAC, CiTCoM, UMR 8038, CNRS Université de Paris, Faculté de Pharmacie, 75006, Paris, France.

The in-depth knowledge of lipid biological functions needs a comprehensive structural annotation including a method to locate fatty acid unsaturations, which remains a thorny problem. For this purpose, we have associated Grubbs' cross-metathesis reaction and liquid chromatography hyphenated to tandem mass spectrometry to locate double bond positions in lipid species. The pretreatment of lipid-containing samples by Grubbs' catalyst and an appropriate alkene generates substituted lipids through cross-metathesis reaction under mild, chemoselective, and reproducible conditions. Read More

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In vitro generation of oxidized standards for lipidomics. Application to major membrane lipid components.

J Chromatogr A 2021 May 19;1651:462254. Epub 2021 May 19.

Centre for Metabolomics and Bioanalysis (CEMBIO), Department of Chemistry and Biochemistry, Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanización Montepríncipe, 28660 Boadilla del Monte, Madrid. Spain. Electronic address:

Membrane lipids (sphingolipids, glycerophospholipids, cardiolipins, and cholesteryl esters) are critical in cellular functions. Alterations in the levels of oxidized counterparts of some of these lipids have been linked to the onset and development of many pathologies. Unfortunately, the scarce commercial availability of chemically defined oxidized lipids is a limitation for accurate quantitative analysis, characterization of oxidized composition, or testing their biological effects in lipidomic studies. Read More

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The structural basis of fatty acid elongation by the ELOVL elongases.

Nat Struct Mol Biol 2021 Jun 10;28(6):512-520. Epub 2021 Jun 10.

Structural Genomics Consortium, Centre for Medicines Discovery, University of Oxford, Oxford, UK.

Very long chain fatty acids (VLCFAs) are essential building blocks for the synthesis of ceramides and sphingolipids. The first step in the fatty acid elongation cycle is catalyzed by the 3-keto acyl-coenzyme A (CoA) synthases (in mammals, ELOVL elongases). Although ELOVLs are implicated in common diseases, including insulin resistance, hepatic steatosis and Parkinson's, their underlying molecular mechanisms are unknown. Read More

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Sphingolipid Δ4-desaturation is an important metabolic step for glycosylceramide formation in Physcomitrium patens.

J Exp Bot 2021 Jun 10. Epub 2021 Jun 10.

University of Goettingen, Albrecht-von-Haller-Institute for Plant Sciences, Department of Plant Biochemistry, Goettingen, Germany.

Glycosylceramides (GlcCers) are abundant membrane components in vascular plants and are associated with cell differentiation, organogenesis, and protein secretion. Long-chain base (LCB) Δ4-desaturation is an important structural feature for metabolic channeling of sphingolipids into GlcCer formation in plants and fungi. In Arabidopsis thaliana LCB Δ4-unsaturated GlcCers are restricted to pollen and floral tissue, indicating that LCB Δ4-desaturation has a less important overall physiological role in A. Read More

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Analytical considerations for reducing the matrix effect for the sphingolipidome quantification in whole blood.

Bioanalysis 2021 Jun 10. Epub 2021 Jun 10.

Department of Systems Pharmacology & Translational Therapeutics, University of Pennsylvania, Philadelphia, PA 19104, USA.

Plasma and serum are widely used blood-derived biofluids for metabolomics and lipidomics assays, but analytes that are present in high concentrations in blood cells cannot be evaluated in those samples and isolating serum or plasma could introduce additional variability in the data. In this study, we provide a comprehensive method for quantification of the whole blood (WB) sphingolipidome, combining a single-phase extraction method with LC-high-resolution mass spectrometry. We were able to quantify more than 150 sphingolipids, and when compared with paired plasma, WB contained higher concentration of most sphingolipids and individual variations were lower. Read More

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Spirulina liquid extract prevents metabolic disturbances and improves liver sphingolipids profile in hamster fed a high-fat diet.

Eur J Nutr 2021 Jun 10. Epub 2021 Jun 10.

INRAe UMR1280 PhAN, Physiopathology of Nutritional Adaptations, CHU Hôtel Dieu, IMAD, CRNH Ouest, University of Nantes, 1, Place Alexis Ricordeau, 44093, Nantes Cedex 01, France.

Purpose: Metabolic syndrome is characterized by hyperglycemia, hyperlipemia and exacerbated oxidative stress. The aim of the study was to determine whether Spirulysat, a Spirulina liquid extract (SLE) enriched in phycocyanin, would prevent metabolic abnormalities induced by high-fat diet.

Methods: The effect of acute SLE supplementation on postprandial lipemia and on triton-induced hyperlipidemia was studied in hamster fed control diet (C). Read More

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(4-Hydroxyphenyl) retinamide suppresses SARS-CoV-2 spike protein-mediated cell-cell fusion by a dihydroceramide Δ4-desaturase 1-independent mechanism.

J Virol 2021 Jun 9:JVI0080721. Epub 2021 Jun 9.

Faculty of Pharma-Science, Teikyo University, Tokyo, Japan.

The membrane fusion between the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and host cells is essential for the initial step of infection; therefore, the host cell membrane components, including sphingolipids, influence the viral infection. We assessed several inhibitors of the enzymes pertaining to sphingolipid metabolism, against SARS-CoV-2 spike protein (S)-mediated cell-cell fusion and viral infection. -(4-hydroxyphenyl) retinamide (4-HPR), an inhibitor of dihydroceramide Δ4-desaturase 1 (DES1), suppressed cell-cell fusion, and viral infection. Read More

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Associations of plasma sphingolipid profiles with insulin response during oral glucose testing in Icelandic horses.

J Vet Intern Med 2021 Jun 8. Epub 2021 Jun 8.

Clinic for Horses, University of Veterinary Medicine Hannover, Foundation, Hanover, Germany.

Background: Sphingolipids modulate insulin sensitivity in mammals. Increased synthesis of ceramides is linked to decreased insulin sensitivity of tissues. Conversely, activation of the insulin signaling pathway can downregulate ceramide synthesis. Read More

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Prospective analysis of circulating metabolites and endometrial cancer risk.

Gynecol Oncol 2021 Jun 5. Epub 2021 Jun 5.

Institute for Risk Assessment Sciences, Utrecht University, Utrecht, the Netherlands.

Background: Endometrial cancer is strongly associated with obesity and dysregulation of metabolic factors such as estrogen and insulin signaling are causal risk factors for this malignancy. To identify additional novel metabolic pathways associated with endometrial cancer we performed metabolomic analyses on pre-diagnostic plasma samples from 853 case-control pairs from the European Prospective Investigation into Cancer and Nutrition (EPIC).

Methods: A total of 129 metabolites (acylcarnitines, amino acids, biogenic amines, glycerophospholipids, hexoses, and sphingolipids) were measured by liquid chromatography-mass spectrometry. Read More

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Profile of sphingolipid-related genes and its association with prognosis highlights sphingolipid metabolism in oral cancer.

Cancer Biomark 2021 May 28. Epub 2021 May 28.

Department of Clinical Analyses, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, SP, Brazil.

Background: Sphingolipids are bioactive lipids that play a role in cancer development. However, the clinical role of sphingolipid (SPL)-related genes in oral cancer (OC) remains not fully understood.

Objective: This study, aimed to examine the mRNA expression of 14 sphingolipid-related genes in oral cancer patients and their implication with clinicopathological features and prognosis. Read More

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Adult diagnosis of congenital serine biosynthesis defect: A treatable cause of progressive neuropathy.

Am J Med Genet A 2021 Jul 4;185(7):2102-2107. Epub 2021 Jun 4.

National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, USA.

A woman with ichthyosis, contractures, and progressive neuropathy represents the first case of phosphoserine aminotransferase deficiency diagnosed and treated in an adult. She has novel compound heterozygous mutations in the gene PSAT1. Treatment with high dose oral L-serine completely resolved the ichthyosis. Read More

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Who's in, who's out? Re-evaluation of lipid raft residents.

J Neurochem 2021 Jun 3. Epub 2021 Jun 3.

Department of Medical Biology and Genetics, Faculty of Medicine, University of Osijek, Osijek, Croatia.

Lipid rafts, membrane microdomains enriched with (glyco)sphingolipids, cholesterol, and select proteins, act as cellular signalosomes. Various methods have been used to separate lipid rafts from bulk (non-raft) membranes, but most often non-ionic detergent Triton X-100 has been used in their isolation. However, Triton X-100 is a reported disruptor of lipid rafts. Read More

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Comprehensive Lipidome and Metabolome Profiling Investigations of and Application in Different Growing Regions Using Liquid Chromatography Coupled with Mass Spectrometry.

J Agric Food Chem 2021 Jun 3;69(23):6710-6719. Epub 2021 Jun 3.

Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.

is one of the most recognized ginseng species. In this study, lipidome and metabolome extraction methods for were optimized with methanol/methyl--butyl ether/water (0.3 mg/1 μL/6 μL/8 μL). Read More

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Discovery of lipid profiles of type 2 diabetes associated with hyperlipidemia using untargeted UPLC Q-TOF/MS-based lipidomics approach.

Clin Chim Acta 2021 May 30;520:53-62. Epub 2021 May 30.

School of Public Health, Zhengzhou University, Zhengzhou 450001, China. Electronic address:

The incidence of type 2 diabetes (T2D) is rising rapidly and has become an important public health problem. According to reports, people with T2D often have hyperlipidemia. Hence, in the current study, a plasma non-targeted lipidomics method was used to study the differences in lipid profile between 36 T2D-associated hyperlipidemia patients and 43 healthy controls by ultra-performance liquid chromatography coupled with quadrupole time-of-flight high-definition mass spectrometry (UPLC Q-TOF/MS). Read More

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Alterations in lipid profile upon uterine fibroids and its recurrence.

Sci Rep 2021 Jun 1;11(1):11447. Epub 2021 Jun 1.

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov of the Ministry of Healthcare of Russian Federation, Moscow, 117997, Russian Federation.

Uterine fibroids (UF) is the most common (about 70% cases) type of gynecological disease, with the recurrence rate varying from 11 to 40%. Because UF has no distinct symptomatology and is often asymptomatic, the specific and sensitive diagnosis of UF as well as the assessment for the probability of UF recurrence pose considerable challenge. The aim of this study was to characterize alterations in the lipid profile of tissues associated with the first-time diagnosed UF and recurrent uterine fibroids (RUF) and to explore the potential of mass spectrometry (MS) lipidomics analysis of blood plasma samples for the sensitive and specific determination of UF and RUF with low invasiveness of analysis. Read More

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Ceramide and Related Molecules in Viral Infections.

Int J Mol Sci 2021 May 26;22(11). Epub 2021 May 26.

Department of Molecular Biology, University of Duisburg-Essen, 45141 Essen, Germany.

Ceramide is a lipid messenger at the heart of sphingolipid metabolism. In concert with its metabolizing enzymes, particularly sphingomyelinases, it has key roles in regulating the physical properties of biological membranes, including the formation of membrane microdomains. Thus, ceramide and its related molecules have been attributed significant roles in nearly all steps of the viral life cycle: they may serve directly as receptors or co-receptors for viral entry, form microdomains that cluster entry receptors and/or enable them to adopt the required conformation or regulate their cell surface expression. Read More

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A Comprehensive Review: Sphingolipid Metabolism and Implications of Disruption in Sphingolipid Homeostasis.

Int J Mol Sci 2021 May 28;22(11). Epub 2021 May 28.

Centre for Neuroscience Studies at Queen's University, Kingston, ON K7L 3N6, Canada.

Sphingolipids are a specialized group of lipids essential to the composition of the plasma membrane of many cell types; however, they are primarily localized within the nervous system. The amphipathic properties of sphingolipids enable their participation in a variety of intricate metabolic pathways. Sphingoid bases are the building blocks for all sphingolipid derivatives, comprising a complex class of lipids. Read More

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Fight Hard or Die Trying: Current Status of Lipid Signaling during Plant-Pathogen Interaction.

Plants (Basel) 2021 May 30;10(6). Epub 2021 May 30.

Department of Botany, Hansraj College, University of Delhi, New Delhi 110007, India.

Plant diseases pose a substantial threat to food availability, accessibility, and security as they account for economic losses of nearly $300 billion on a global scale. Although various strategies exist to reduce the impact of diseases, they can introduce harmful chemicals to the food chain and have an impact on the environment. Therefore, it is necessary to understand and exploit the plants' immune systems to control the spread of pathogens and enable sustainable agriculture. Read More

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The Role of Fatty Acids in Ceramide Pathways and Their Influence on Hypothalamic Regulation of Energy Balance: A Systematic Review.

Int J Mol Sci 2021 May 19;22(10). Epub 2021 May 19.

Faculty of Applied Science, University of Campinas, UNICAMP, Campinas 13484-350, Brazil.

Obesity is a global health issue for which no major effective treatments have been well established. High-fat diet consumption is closely related to the development of obesity because it negatively modulates the hypothalamic control of food intake due to metaflammation and lipotoxicity. The use of animal models, such as rodents, in conjunction with in vitro models of hypothalamic cells, can enhance the understanding of hypothalamic functions related to the control of energy balance, thereby providing knowledge about the impact of diet on the hypothalamus, in addition to targets for the development of new drugs that can be used in humans to decrease body weight. Read More

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The Role of Ceramide Metabolism and Signaling in the Regulation of Mitophagy and Cancer Therapy.

Cancers (Basel) 2021 May 19;13(10). Epub 2021 May 19.

Hollings Cancer Center, Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA.

Sphingolipids are bioactive lipids responsible for regulating diverse cellular functions such as proliferation, migration, senescence, and death. These lipids are characterized by a long-chain sphingosine backbone amide-linked to a fatty acyl chain with variable length. The length of the fatty acyl chain is determined by specific ceramide synthases, and this fatty acyl length also determines the sphingolipid's specialized functions within the cell. Read More

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Sphingosine 1 Phosphate (S1P) Receptor 1 Is Decreased in Human Lung Microvascular Endothelial Cells of Smokers and Mediates S1P Effect on Autophagy.

Cells 2021 May 14;10(5). Epub 2021 May 14.

Department of Medicine, Division of Pulmonary, Critical Care, and Sleep Medicine, National Jewish Health, Denver, CO 80206, USA.

Destruction of alveoli by apoptosis induced by cigarette smoke (CS) is a major driver of emphysema pathogenesis. However, when compared to cells isolated from non-smokers, primary human lung microvascular endothelial cells (HLMVECs) isolated from chronic smokers are more resilient when exposed to apoptosis-inducing ceramide. Whether this adaptation restores homeostasis is unknown. Read More

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Genome-Wide Association Study Provides Insights into Important Genes for Reproductive Traits in Nelore Cattle.

Animals (Basel) 2021 May 13;11(5). Epub 2021 May 13.

Departamento de Engenharia e Ciências Exatas, Universidade Estadual Paulista, Jaboticabal 14884-900, São Paulo, Brazil.

The identification of genomic regions associated with reproductive traits as well as their biological processes allows a better understanding of the phenotypic variability of these traits. This information could be applied to animal breeding programs to accelerate genetic gain. The aim of this study was to evaluate the association between single nucleotide polymorphisms (SNP) with a scrotal circumference at 365 days of age (SC365) and at 450 days of age (SC450), gestation length (GL) as a calf trait, age at first calving (AFC), accumulated productivity (ACP), heifer early calving until 30 months (HC30), and stayability (STAY) traits, in order to identify candidate genes and biological pathways associated with reproductive traits in Nelore cattle. Read More

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Gangliosides as Signaling Regulators in Cancer.

Int J Mol Sci 2021 May 11;22(10). Epub 2021 May 11.

Division of Aging and Carcinogenesis, Research Team for Geriatric Pathology, Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan.

At the plasma membrane, gangliosides, a group of glycosphingolipids, are expressed along with glycosphingolipids, phospholipids, and cholesterol in so-called lipid rafts that interact with signaling receptors and related molecules. Most cancers present abnormalities in the intracellular signal transduction system involved in tumor growth, invasion, and metastasis. To date, the roles of gangliosides as regulators of signal transduction have been reported in several cancer types. Read More

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Untargeted Lipidomic Profiling of Dry Blood Spots Using SFC-HRMS.

Metabolites 2021 May 11;11(5). Epub 2021 May 11.

MetaboHUB-MetaToul-Lipidomique, MetaboHUB-ANR-11-INBS-0010, Inserm U1297/Université Paul Sabatier Toulouse III, 31432 Toulouse, France.

Lipids are essential cellular constituents that have many critical roles in physiological functions. They are notably involved in energy storage and cell signaling as second messengers, and they are major constituents of cell membranes, including lipid rafts. As a consequence, they are implicated in a large number of heterogeneous diseases, such as cancer, diabetes, neurological disorders, and inherited metabolic diseases. Read More

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The Anti-Infectious Role of Sphingosine in Microbial Diseases.

Cells 2021 05 4;10(5). Epub 2021 May 4.

Department of Molecular Biology, University of Duisburg-Essen, Hufelandstrasse 55, 45122 Essen, Germany.

Sphingolipids are important structural membrane components and, together with cholesterol, are often organized in lipid rafts, where they act as signaling molecules in many cellular functions. They play crucial roles in regulating pathobiological processes, such as cancer, inflammation, and infectious diseases. The bioactive metabolites ceramide, sphingosine-1-phosphate, and sphingosine have been shown to be involved in the pathogenesis of several microbes. Read More

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Biosynthesis and Functions of Very-Long-Chain Fatty Acids in the Responses of Plants to Abiotic and Biotic Stresses.

Cells 2021 May 21;10(6). Epub 2021 May 21.

University of Bordeaux, CNRS, Laboratoire de Biogenèse Membranaire, UMR 5200, F-33140 Villenave d'Ornon, France.

Very-long-chain fatty acids (i.e., fatty acids with more than 18 carbon atoms; VLCFA) are important molecules that play crucial physiological and structural roles in plants. Read More

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Ceramide Synthase 6 Maximizes p53 Function to Prevent Progeny Formation from Polyploid Giant Cancer Cells.

Cancers (Basel) 2021 May 5;13(9). Epub 2021 May 5.

Department of Microbiology & Immunology, Medical University of South Carolina, Charleston, SC 29425, USA.

Polyploid giant cancer cells (PGCC) constitute a transiently senescent subpopulation of cancer cells that arises in response to stress. PGCC are capable of generating progeny via a primitive, cleavage-like cell division that is dependent on the sphingolipid enzyme acid ceramidase (ASAH1). The goal of this study was to understand differences in sphingolipid metabolism between non-polyploid and polyploid cancer cells to gain an understanding of the ASAH1-dependence in the PGCC population. Read More

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Regulation of sphingolipid synthesis by the G1/S transcription factor Swi4.

Biochim Biophys Acta Mol Cell Biol Lipids 2021 May 29;1866(9):158983. Epub 2021 May 29.

Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address:

SBF (Swi4/Swi6 Binding Factor) complex is a crucial regulator of G1/S transition in Saccharomyces cerevisiae. Here, we show that SBF complex is required for myriocin resistance, an inhibitor of sphingolipid synthesis. This phenotype was not shared with MBF complex mutants nor with deletion of the Swi4p downstream targets, CLN1/CLN2. Read More

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Three-Dimensional Imaging of Whole-Body Zebrafish Revealed Lipid Disorders Associated with Niemann-Pick Disease Type C1.

Anal Chem 2021 Jun 1;93(23):8178-8187. Epub 2021 Jun 1.

Guangzhou Key Laboratory of Environmental Catalysis and Pollution Control, Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control, School of Environmental Science and Engineering, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou 510006, China.

Imaging of lipids of whole-body specimens in two-dimensional (2D) analysis provides a global picture of the lipid changes in lipid-disturbed diseases, enabling a better understanding of lipid functions and lipid-modulation processes in different organs. However, 2D imaging of a single cross section can hardly characterize the whole-body lipid alterations. In this work, a three-dimensional matrix-assisted laser desorption/ionization mass spectrometry imaging (3D MALDI-MSI) approach was developed for analysis of whole-body zebrafish, for the first time, and applied to identify altered lipids and map their spatial distributions by using a zebrafish model of Niemann-Pick disease type C1 (NPC1), a neurovisceral lipid storage disorder causing both neurodegenerative disorder and visceral organ damage. Read More

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My Journey in Academia as a Lipid Biochemist.

Cell Biochem Biophys 2021 May 31. Epub 2021 May 31.

Departments of Pharmacology & Regenerative Medicine and Medicine, University of Illinois, Chicago, IL, 60612, USA.

An overview of Prof. Viswanathan Natarajan's journey in academia as a mentor, teacher, and lipid scientist for nearly 50 years is presented. As a graduate student, Dr. Read More

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