759 results match your criteria spermatogenesis steroidogenesis

Activation of C-C motif chemokine receptor 2 modulates testicular macrophages number, steroidogenesis, and spermatogenesis progression.

Cell Tissue Res 2021 Jul 23. Epub 2021 Jul 23.

Laboratory of Cellular Biology, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.

The monocyte chemoattractant protein 1 (MCP-1) belongs to the CC chemokine family and acts in the recruitment of C-C motif chemokine receptor 2 (CCR2)-positive immune cell types to inflammation sites. In testis, the MCP-1/CCR2 axis has been associated with the macrophage population's functional regulation, which presents significant functions supporting germ cell development. In this context, herein, we aimed to investigate the role of the chemokine receptor CCR2 in mice testicular environment and its impact on male sperm production. Read More

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Prolonged use of letrozole causes morphological changes on gonads in .

Anim Reprod 2021 12;18(2):e20200029. Epub 2021 Jul 12.

Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brasil.

Letrozole is used as a therapeutic agent in reproductive disorders caused by high estrogen levels. Letrozole inhibits cytochrome P450 aromatase and reduces estrogen levels. However, the effects of long-term use on reproductive traits are unknown. Read More

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Bee bread mitigates down-regulation of steroidogenic genes, decreased spermatogenesis, and epididymal oxidative stress in male rats fed with high-fat diet.

Am J Physiol Endocrinol Metab 2021 07 6. Epub 2021 Jul 6.

Department of Physiology, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia.

Background: The pituitary-gonadal axis plays an important role in steroidogenesis and spermatogenesis and by extension, fertility.

Objectives: The aim of this study was to investigate the protective role of bee bread, which is a natural bee product, against obesity-induced decreases in steroidogenesis and spermatogenesis.

Methods: Thirty-two adult male Sprague-Dawley rats weighing between 200-300 g were divided into four groups (n=8/group), namely: normal control (NC), high-fat diet (HFD), HFD plus bee bread administered concurrently for 12 weeks (HFD+B), HFD plus orlistat administered concurrently for 12 weeks (HFD+O) groups. Read More

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GC-MS metabolomics reveals dysregulated lipid metabolic pathways and metabolites in diabetic testicular toxicity: Therapeutic potentials of raffia palm (Raphia hookeri G. Mann & H. Wendl) wine.

J Ethnopharmacol 2021 Jul 2;279:114390. Epub 2021 Jul 2.

Department of Biochemistry, School of Life Sciences, University of KwaZulu-Natal (Westville Campus), Durban, 4000, South Africa. Electronic address:

Ethnopharmacological Relevance: Raffia palm (Raphia hookeri G. Mann & H. Wendl) wine (RPW) is a natural beverage obtained from the R. Read More

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The Aromatase-Estrogen System in the Testes of Non-Mammalian Vertebrates.

Animals (Basel) 2021 Jun 12;11(6). Epub 2021 Jun 12.

Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Università degli Studi della Campania "Luigi Vanvitelli", 81100 Caserta, Italy.

Estrogens are important physiological regulators of testicular activity in vertebrates. Estrogen levels depend on the activity of P450 aromatase, the enzyme responsible for the irreversible conversion of testosterone into 17β-estradiol. Therefore, P450 aromatase is the key player in the aromatase-estrogen system. Read More

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Dietary supplementation of grape seed tannin extract stimulated testis development, changed fatty acid profiles and increased testis antioxidant capacity in pre-puberty hu lambs.

Theriogenology 2021 Jun 20;172:160-168. Epub 2021 Jun 20.

State Key Laboratory of Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, China; Gansu Runmu Biological Engineering Co., Ltd., Yongchang, 737200, China; Biotechnology Engineering Laboratory of Gansu Meat Sheep Breeding, Minqin, 733300, China. Electronic address:

Grape seed tannin extract (GPE) from wine grape pomace has many effective anti-oxidative effects and is used as a promising natural feed additive in the animal feed industry. This study investigated the effect of GPE as a source of tannin on the antioxidant capacity and testis development in Hu lambs. Twenty-seven 3-month-old ram lambs were randomly assigned to three groups. Read More

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The effect of Apium graveolens L., Levisticum officinale and Calendula officinalis L. on cell viability, membrane integrity, steroidogenesis, and intercellular communication in mice Leydig cells in vitro.

Physiol Res 2021 Jun 1. Epub 2021 Jun 1.

BioFood Centre, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic.

Several plants have the potential to protect essential reproductive processes such as spermatogenesis or steroidogenesis, however, effective concentrations and main mechanisms of action are still unknown. This in vitro study was aimed to assess the effects of Apium graveolens L., Levisticum officinale, and Calendula officinalis L. Read More

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Stereological analysis and transcriptome profiling of testicular injury induced by di-(2-ethylhexyl) phthalate in prepubertal rats.

Ecotoxicol Environ Saf 2021 Sep 18;220:112326. Epub 2021 May 18.

Department of Urology, Children's Hospital of Chongqing Medical University, Chongqing 400014, PR China; Chongqing Key Laboratory of Children Urogenital Development and Tissue Engineering, Chongqing 400014, PR China; Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing 400014, PR China; National Clinical Research Center for Child Health and Disorders, Chongqing 400014, PR China; China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing 400014, PR China; Chongqing Key Laboratory of Pediatrics, Chongqing 400014, PR China. Electronic address:

Di-(2-ethylhexyl) phthalate (DEHP) is the most common phthalate that can affect the male reproductive system. DEHP exposure at the prepubertal stage could lead to the injury of immature testes, but the mechanism has not been fully clarified. In the present study, we elucidated the possible underlying mechanism of DEHP-induced prepubertal testicular injury through stereological analysis and transcriptome profiling. Read More

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September 2021

Experimental Cryptorchidism Causes Chronic Inflammation and a Progressive Decline in Sertoli Cell and Leydig Cell Function in the Adult Rat Testis.

Reprod Sci 2021 May 18. Epub 2021 May 18.

Centre for Reproductive Health, Hudson Institute of Medical Research, Clayton, Victoria, Australia.

Cryptorchidism causes spermatogenic failure and reduced serum androgen levels, as well as testicular oedema and fibrosis, which are hallmarks of inflammation. However, the role of inflammation and the effects of cryptorchidism on Sertoli cell and Leydig cell function at the molecular level remain ill-defined. Bilateral cryptorchidism was surgically induced in adult rats for 7 and 14 weeks. Read More

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Myristic acid defends against testicular oxidative stress, inflammation, apoptosis: Restoration of spermatogenesis, steroidogenesis in diabetic rats.

Life Sci 2021 Aug 11;278:119605. Epub 2021 May 11.

Department of Physiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address:

Diabetes mellitus (DM) may lead to testicular-related infertility while Myristic acid (MA) is beneficial to lower hyperglycaemia. Thus, we hypothesized that MA could protect testes against hyperglycaemia-induced damage in DM. DM was induced in adult male rats by high-fat diet consumption for 12 weeks, accompanied by a single dose streptozotocin injection. Read More

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L-carnitine extenuates endocrine disruption, inflammatory burst and oxidative stress in carbendazim-challenged male rats via upregulation of testicular StAR and FABP9, and downregulation of P38-MAPK pathways.

Toxicology 2021 06 15;457:152808. Epub 2021 May 15.

Department of Pharmacology & Toxicology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.

We have addressed in the current study the potential of L-carnitine (LC) to extenuate the reproductive toxic insults of carbendazim (CBZ) in male rats, and the molecular mechanisms whereby carnitine would modify the spermatogenic and steroidogenic derangements invoked by the endocrine disruptor. Herein, animals received daily doses of carbendazim (100 mg/kg) by gavage for 8 weeks. Another CBZ-challenged group was co-supplemented with LC (500 mg/kg, IP) twice weekly for 8 weeks. Read More

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Melatonin Ameliorates LPS-Induced Testicular Nitro-oxidative Stress (iNOS/TNFα) and Inflammation (NF-kB/COX-2) via Modulation of SIRT-1.

Reprod Sci 2021 Apr 30. Epub 2021 Apr 30.

Pineal Research Lab Reproduction Biology Unit, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi, Uttar Pradesh, 221005, India.

Lipopolysaccharide (LPS) - an endotoxin that is being extensively used in laboratory to mimic microbial infection that adversely affects male fertility. This study investigated the protective effects of melatonin on LPS-induced testicular nitro-oxidative stress, inflammation, and associated damages in the testes of male golden hamsters, Mesocricetus auratus. Hamsters were administered with melatonin and LPS for 7 days. Read More

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A Testis-Specific Long Noncoding RNA, , Is a Regulator of Steroidogenesis in Mouse Leydig Cells.

Front Endocrinol (Lausanne) 2021 1;12:665874. Epub 2021 Apr 1.

Graduate School of Life Science, Hokkaido University, Sapporo, Japan.

The testis expresses many long noncoding RNAs (lncRNAs), but their functions and overview of lncRNA variety are not well understood. The mouse locus contains six serine protease genes and two lncRNAs that have been suggested to play important roles in spermatogenesis. Here, we found a novel testis-specific lncRNA, (), in this locus. Read More

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Safflower seed oil improves steroidogenesis and spermatogenesis in rats with type II diabetes mellitus by modulating the genes expression involved in steroidogenesis, inflammation and oxidative stress.

J Ethnopharmacol 2021 Jul 22;275:114139. Epub 2021 Apr 22.

Department of Radiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Students' Scientific Research Center (SSRC), Tehran University of Medical Sciences, Tehran, Iran. Electronic address:

Ethnopharmacological Relevance: Diabetes mellitus (DM), as a multiorgan syndrome, is an endocrine and metabolic disorder that is associated with male reproductive system dysfunction and infertility. Safflower (Carthamus tinctorius L.) as an herbal remedy improves DM and infertility-related disorders. Read More

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Leydig Cell-Specific DAX1-Deleted Mice Has Higher Testosterone Level in the Testis During Pubertal Development.

Reprod Sci 2021 Apr 23. Epub 2021 Apr 23.

School of Biological Sciences and Technology, Chonnam National University, Gwangju, Republic of Korea.

Testosterone, the male sex hormone, is necessary for the development and function of the male reproductive system. Biosynthesis of testosterone in mammals mainly occurs in testicular Leydig cells. Many proteins such as P450c17, 3β-HSD, and StAR are involved in testicular steroidogenesis. Read More

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The Effect of Metformin on Male Reproductive Function and Prostate: An Updated Review.

Chin Hsiao Tseng

World J Mens Health 2021 Mar 9. Epub 2021 Mar 9.

Department of Internal Medicine, National Taiwan University College of Medicine, Taipei, Taiwan.

Metformin is the first-line oral antidiabetic drug that shows multiple pleiotropic effects of anti-inflamation, anti-cancer, anti-aging, anti-microbia, anti-atherosclerosis, and immune modulation. Metformin's effects on men's related health are reviewed here, focusing on reproductive health under subtitles of erectile dysfunction (ED), steroidogenesis and spermatogenesis; and on prostate-related health under subtitles of prostate specific antigen (PSA), prostatitis, benign prostate hyperplasia (BPH), and prostate cancer (PCa). Updated literature suggests a potential role of metformin on arteriogenic ED but controversial and contradictory effects (either protective or harmful) on testicular functions of testosterone synthesis and spermatogenesis. Read More

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Is there a role for glucagon-like peptide-1 receptor agonists in the treatment of male infertility?

Andrology 2021 Apr 5. Epub 2021 Apr 5.

Department of Clinical and Experimental Medicine, University of Catania, Catania, Italy.

Glucagon-like peptide-1 receptor agonists (GLP1-RAs) are novel anti-hyperglycemic drugs efficacious on glucose control, weight loss, and cardiovascular prevention. These drugs may also be effective in modulating testicular function. In fact, they increase serum testosterone levels in diabetic and/or obese patients with functional hypogonadism on a dysmetabolic basis. Read More

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The crosstalk between melatonin and sex steroid hormones.

Neuroendocrinology 2021 Mar 26. Epub 2021 Mar 26.

Melatonin, an indolamine mainly released from the pineal gland, is associated with many biological functions namely the modulation of circadian and seasonal rhythms, sleep inducer, regulator of energy metabolism, antioxidant and anticarcinogenic. Although several evidences also recognize the influence of melatonin in the reproductive physiology, the crosstalk between melatonin and sex hormones is not clear. Here, we review the effects of sex differences in the circulating levels of melatonin and update the current knowledge on the link between sex hormones and melatonin. Read More

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A local regulatory network in the testis mediated by laminin and collagen fragments that supports spermatogenesis.

Crit Rev Biochem Mol Biol 2021 Jun 25;56(3):236-254. Epub 2021 Mar 25.

The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, China.

It is almost five decades since the discovery of the hypothalamic-pituitary-testicular axis. This refers to the hormonal axis that connects the hypothalamus, pituitary gland and testes, which in turn, regulates the production of spermatozoa through spermatogenesis in the seminiferous tubules, and testosterone through steroidogenesis by Leydig cells in the interstitium, of the testes. Emerging evidence has demonstrated the presence of a regulatory network across the seminiferous epithelium utilizing bioactive molecules produced locally at specific domains of the epithelium. Read More

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Chlorella vulgaris or Spirulina platensis mitigate lead acetate-induced testicular oxidative stress and apoptosis with regard to androgen receptor expression in rats.

Environ Sci Pollut Res Int 2021 Aug 22;28(29):39126-39138. Epub 2021 Mar 22.

Department of Biochemistry, Faculty of Vet. Medicine, Suez Canal University, Ismailia, 41522, Egypt.

The current research was constructed to throw the light on the protective possibility of Chlorella vulgaris (C. vulgaris) and Spirulina platensis (S. platensis) against lead acetate-promoted testicular dysfunction in male rats. Read More

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Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats.

Sci Rep 2021 Mar 9;11(1):5522. Epub 2021 Mar 9.

Department of Physiology, School of Medical Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia.

Doxorubicin (DOX) is a broad-spectrum chemotherapeutic drug used in the treatment of cancers. It acts by generating reactive oxygen species in target cells. The actions are, however, not limited to cancerous cells as it attacks healthy cells, killing them. Read More

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The possible role of sirtuins in male reproduction.

Mol Cell Biochem 2021 Jul 18;476(7):2857-2867. Epub 2021 Mar 18.

Molecular Endocrinology Laboratory, Department of Biochemistry, Centre for Excellence in Life Sciences, Bharathidasan University, Tiruchirappalli, 620 024, India.

Global influence of male infertility is increasing in recent decades. Proper understanding of genetics, anatomy, physiology and the intricate interrelation of male reproductive system are much needed for explaining the etiology of male infertility; and a detailed study on the epigenetics, indeed, will reveal the molecular mechanism behind its etiology. Sirtuins, the molecular sensors, are NAD dependent histone deacetylases and ADP- ribosyl transferases, participate in the chief events of epigenetics. Read More

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Kobayashi Award 2019: The neuroendocrine regulation of the mammalian reproduction.

Hiroko Tsukamura

Gen Comp Endocrinol 2021 Mar 10:113755. Epub 2021 Mar 10.

Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan. Electronic address:

Mammalian reproductive function is a complex system of many players orchestrated by the hypothalamus-pituitary-gonadal (HPG) axis. The hypothalamic gonadotropin-releasing hormone (GnRH) and the consequent pituitary gonadotropin release show two modes of secretory patterns, namely the surge and pulse modes. The surge mode is triggered by the positive feedback action of estrogen secreted from the mature ovarian follicle to induce ovulation in females of most mammalian species. Read More

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Effects of SPATA6 on proliferation, apoptosis and steroidogenesis of Hu sheep Leydig cells in vitro.

Theriogenology 2021 May 20;166:9-20. Epub 2021 Feb 20.

Jiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing, 210095, China; Hu Sheep Academy, Nanjing Agricultural University, Nanjing, 210095, China; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address:

This study aimed to investigate the expression pattern of spermatogenesis associated protein 6 (SPATA6) in Hu sheep testis and to ascertain the effects of SPATA6 on sheep Leydig cells (LCs) function linked to spermatogenesis. In the present study, we detected a 1970 bp cDNA fragment of SPATA6 included a 1467 bp coding sequence which encoded 487 amino acids. Meanwhile, sheep SPATA6 shared 51. Read More

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Hindlimb unloading-induced reproductive suppression via Downregulation of hypothalamic Kiss-1 expression in adult male rats.

Amira Moustafa

Reprod Biol Endocrinol 2021 Mar 4;19(1):37. Epub 2021 Mar 4.

Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44519, Egypt.

Background: Spaceflights-induced microgravity can alter various physiological processes in human's body including the functional status of the reproductive system. Rodent model of tail-suspension hindlimb unloading is extensively used to stimulate the organs responses to the microgravity condition. This study explores the potential effects of hindlimb unloading on testicular functions and spermatogenesis in adult male rats and the underlying mechanism/s. Read More

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β-carotene Rescues Busulfan Disrupted Spermatogenesis Through Elevation in Testicular Antioxidant Capability.

Front Pharmacol 2021 15;12:593953. Epub 2021 Feb 15.

College of Animal Sciences and Technology, Qingdao Agricultural University, Qingdao, China.

β-carotene, precursor of vitamin A, is an excellent antioxidant with many beneficial properties. It is a lipid-soluble antioxidant and a very effective quencher of reactive oxygen species (ROS) to reduce the oxidative stress. In contrast to vitamin A, β-carotene is not toxic even consumed in higher amount when it is delivered from natural plant products. Read More

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February 2021

Rescue effect of sodium acetate in diabetes mellitus-associated testicular dysfunction is accompanied by PCSK9 modulation.

Biochimie 2021 May 10;184:52-62. Epub 2021 Feb 10.

HOPE Cardiometabolic Research Team & Department of Physiology, College of Health Sciences, University of Ilorin, Ilorin, 240001, Nigeria.

Diabetes mellitus (DM) is a global health burden, affecting about 463 million of the adult population worldwide. Approximately 94% of diabetic male individuals develop varying degrees of testicular disorders (TDs), which usually result in hypogonadism, hypotestosteronemia and defective spermatogenesis and steroidogenesis. Short chain fatty acids (SCFAs) have shown potential benefits in metabolic health. Read More

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Longitudinal Expression of Testicular from Prepuberty to Sexual Maturity in Congjiang Xiang Pigs.

Animals (Basel) 2021 Feb 8;11(2). Epub 2021 Feb 8.

College of Animal Science, Guizhou University, Guiyang 550025, China.

Testicular expression of taste receptor type 1 subunit 3 (T1R3), a sweet/umami taste receptor, has been implicated in spermatogenesis and steroidogenesis in mice. We explored the role of testicular T1R3 in porcine postnatal development using the Congjiang Xiang pig, a rare Chinese miniature pig breed. Based on testicular weights, morphology, and testosterone levels, four key developmental stages were identified in the pig at postnatal days 15-180 (prepuberty: 30 day; early puberty: 60 day; late puberty: 90 day; sexual maturity: 120 day). Read More

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February 2021

Oyster peptide prevents the occurrence of exercise-hypogonadal male condition by improving the function of pituitary gonadal axis in male rats.

Andrologia 2021 May 10;53(4):e14005. Epub 2021 Feb 10.

Beijing Engineering Research Center of Protein and Functional Peptides, China National Research Institute of Food and Fermentation Industries Co., Ltd., Beijing, China.

This study evaluates the protective role of oyster peptide (OP) on the occurrence of Exercise-Hypogonadal Male Condition. Male rats were given heavy-load swimming training and / or OP was supplemented for 6 consecutive weeks. After heavy-load training, sperm count, sperm viability and sperm motility in epididymis, testosterone in serum and testis, glutathione peroxidase (GSH-px) and androgen receptor (AR) in testis and mating times were remarkably decreased, malondialdehyde (MDA), capture latency and mating latency were significantly increased, mRNA expression of steroidogenic acute regulatory (StAR) and P450 cholesterol side-chain cleavage enzyme (P450scc) were obviously down-regulated, but serum follicle-stimulating hormone (FSH) and luteinising hormone (LH) were not statistically changed. Read More

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induced testicular assault through Leydig cell derangement: an approach.

J Complement Integr Med 2021 Jan 22. Epub 2021 Jan 22.

Department of Physiology, Harimohan Ghose College (affiliated to University of Calcutta), J206, Paharpur Road, Garden Reach, Kolkata 700024, West Bengal, India.

Objectives: (TA) has been widely used as folk medicine since ancient times. Apart from its therapeutic properties it also has anti-spermatogenic activity, but its effectiveness and mode of action on male gonadal activity remains to be revealed.

Methods: TA bark extract was dissolved in 1% dimethyl sulfoxide and applied in primary Leydig cell culture from rat testis in dose dependent manner. Read More

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January 2021