7,351 results match your criteria sperm collected

Effect of bariatric surgery on in vitro fertilization in infertile men with obesity.

Surg Obes Relat Dis 2021 Jul 9. Epub 2021 Jul 9.

Department of Advanced Biomedical Sciences, University of Naples "Federico II", Naples, Italy. Electronic address:

Background: Obesity has previously been related to reduced female fertility, with prolonged waiting time to pregnancy among women with a body mass index (BMI) >35 kg/m but there are few studies investigating the relationship between high BMI, bariatric surgery, and male fertility.

Objectives: The primary objective of this article was to investigate the effect of bariatric surgery on in vitro fertilization (IVF) outcomes in a cohort of men with morbid obesity who underwent sleeve gastrectomy (SG).

Setting: University hospital, bariatric surgery unit. Read More

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TLR7/8 signalling affects X-sperm motility via the GSK3 α/β-hexokinase pathway for the efficient production of sexed dairy goat embryos.

J Anim Sci Biotechnol 2021 Aug 3;12(1):89. Epub 2021 Aug 3.

Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi, 712100, People's Republic of China.

Background: Goat milk is very similar to human milk in terms of its abundant nutrients and ease of digestion. To derive greater economic benefit, farmers require more female offspring (does); however, the buck-to-doe offspring sex ratio is approximately 50%. At present, artificial insemination after the separation of X/Y sperm using flow cytometry is the primary means of controlling the sex of livestock offspring. Read More

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First successful frozen semen of the maned wolf (Chrysocyon brachyurus).

Reprod Domest Anim 2021 Aug 2. Epub 2021 Aug 2.

Department of Veterinary Surgery and Animal Reproduction, FMVZ, São Paulo State University (UNESP), Botucatu, Brazil.

This study aimed to describe successful cryopreservation of sperm from maned wolves (Chrysocyon brachyurus). Three ejaculates from 2 maned wolves were collected by digital manipulation of the penis and evaluated subjectively, centrifuged, and frozen in BotuCrio (Botupharma, Botucatu, Brazil) or TRIS - yolk egg extender. Spermatozoa were thawed at 37 ºC/30s or 70 ºC/4s and evaluated for kinetics, morphology, plasma and acrosome membrane integrity, mitochondrial potential, hydrogen peroxide, superoxide anion, and lipid peroxidation. Read More

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The impact of antisperm antibodies on human male reproductive function: an update.

Reproduction 2021 Aug 1. Epub 2021 Aug 1.

S Amaral, University of Coimbra, University of Coimbra Center for Neuroscience and Cell Biology, Coimbra, Portugal.

Immune infertility occurs due to the presence of antisperm antibodies (ASA). This type of infertility has a relatively low prevalence (2.6-6. Read More

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Seminal plasma metabolomics profiles following long (4-7 days) and short (2 h) sexual abstinence periods.

Eur J Obstet Gynecol Reprod Biol 2021 Jul 15;264:178-183. Epub 2021 Jul 15.

Department of Medical Biosciences, University of the Western Cape, Cape Town, South Africa.

Objective: Metabolomic profiling of seminal plasma has been suggested as a possible approach for a fast and non-invasive male infertility evaluation diagnosis. However, metabolomics profiles in normozoospermic men have not been thoroughly investigated, and the influence of ejaculation-abstinence has not been described. To provide interim reference values and find associations between the metabolomics profiles of human seminal plasma and length of ejaculation-abstinence period in normozoospermic men. Read More

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Precocious puberty in male wild boars: a possible explanation for the dramatic population increase in Germany and Europe.

PeerJ 2021 20;9:e11798. Epub 2021 Jul 20.

Institute for Anatomy, University of Veterinary Medicine Hannover Foundation, Hannover, Germany.

Background: The wild boar population in Europe is steadily growing, one of the reasons for this increase probably being the high reproductive potential of this large mammal. Population management is important to stabilise wild boar numbers and a great deal of attention is focusing on the reasons, which might contribute to the high reproductive rates. Understanding the timing of puberty attainment provides information required for proper management practices. Read More

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ethanol extract prevents monosodium glutamate adverse effects on the serum levels of reproductive hormones, sperm quality, and testis histology in male rats.

Vet World 2021 Jun 15;14(6):1529-1536. Epub 2021 Jun 15.

Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang 65145, East Java, Indonesia.

Background And Aim: is an aquatic plant that contains high antioxidants level and could prevent cell damages caused by free radicals. The present study aimed to investigate the effect of . ethanol extract on luteinizing hormone (LH), testosterone levels, sperm quality, and testis histology of adult male rats induced by monosodium glutamate (MSG). Read More

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Seasonal effect on semen availability and quality of racing pigeon in Thailand.

Vet World 2021 Jun 7;14(6):1459-1464. Epub 2021 Jun 7.

Faculty of Veterinary Medicine, Rajamangala University of Technology Tawan-Ok, Chonburi, Thailand.

Background And Aim: Seasonal variations among geographical regions could influence pigeon semen quality differently. This study aimed to determine the seasonal effect on semen availability and quality of racing pigeons in Thailand to understand and improve breeding management in the country.

Materials And Methods: Semen was collected from six fertile captive pigeons once a week during summer (March-June), monsoon (July-October), and winter (November-February) during 2019-2020. Read More

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Effects of spp., , and seeds oils on quality of chilled and frozen-thawed ram semen.

Vet World 2021 Jun 3;14(6):1412-1419. Epub 2021 Jun 3.

Departamento de Reproducción animal del HUMECO, C/Mecanica 11. 22006 Huesca. Spain.

Background And Aim: Essential oils found frequently in plants are well known for their activities against bacteria, viruses, and fungi, and antioxidant properties. This study aimed to analyze egg yolk replacement by seed oils of spp. (cotton), (desert date), and (sesame) in semen extender, on ram sperm quality chilled at 4°C and frozen-thawed. Read More

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Improving the post-thaw quality of rooster semen using the extender supplemented with resveratrol.

Poult Sci 2021 May 27;100(9):101290. Epub 2021 May 27.

Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute Reproductive Biomedicine, ACECR, Tehran, Iran.

Avian spermatozoa are highly susceptible to reactive oxygen species (ROS) produced during the cryopreservation. The aim of the current study was to investigate the antioxidant effects of resveratrol (RSV) during rooster semen cryopreservation. Changes in expression of AMP-activated protein kinase as a possible mechanism behind the beneficial effects of resveratrol were also evaluated. Read More

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Risk factors of pregnancy failure in elderly infertility patients undergoing human assisted reproductive technology.

Am J Transl Res 2021 15;13(6):7306-7311. Epub 2021 Jun 15.

Reproductive Medicine, Maternal and Child Health Hospital of Hubei Province Wuhan, Hubei Province, China.

Objective: To investigate the risk factors of pregnancy failure in elderly infertile patients undergoing human assisted reproductive technology (ART).

Methods: A total of 565 infertile patients undergoing ART were selected and divided into failed pregnancy group (127 cases) and continued pregnancy group (438 cases). Their clinical data were collected, and the influencing factors of pregnancy failure were assessed and compared by univariate and multivariate analysis. Read More

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Low-level germline mosaicism of a novel SMARCA2 missense variant: Expanding the phenotypic spectrum and mode of genetic transmission.

Mol Genet Genomic Med 2021 Jul 22:e1763. Epub 2021 Jul 22.

Shanghai Key Laboratory of Embryo Original Diseases, Shanghai, China.

Background: Nicolaides-Baraitser syndrome (NCBRS) is a severe neurodevelopmental disorder with multiple abnormalities. To date, all pathogenic variants in SMARCA2 causing NCBRS are de novo and most are missense variants located in the ATPase domain of SMARCA2 protein.

Methods: In this study, a familial trio whole-exome sequencing was performed on the proband presenting with intellectual disability, early-onset epilepsy, and autistic features. Read More

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induced sperm DNA damage on the experimentally infected rats.

J Parasit Dis 2021 Jun 10;45(2):351-358. Epub 2020 Nov 10.

Department of Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, P.O. Box: 65157838736, Hamadan, Iran.

, as an obligate protozoan parasite, can infect a wide variety of animals as well as human. As some studies have shown, toxoplasmosis decreases the fertility potency in different hosts, so there is a necessity for studies to determine the effects of on reproductive system. Therefore, this project was aimed to investigate the effect of toxoplasmosis on the male reproductive system and sperm DNA integrity. Read More

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Injection funnel persistence time and oolemma resistance during intracytoplasmic sperm injection and subsequent embryo development.

J Obstet Gynaecol Res 2021 Jul 20. Epub 2021 Jul 20.

Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, South Korea.

Objective: This study aimed to investigate whether the injection funnel persistence time and oolemma resistance during the intracytoplasmic sperm injection (ICSI) are associated with subsequent embryo quality.

Design: A prospective observational study at a university hospital.

Methods: One hundred and twenty normal-appearing metaphase II oocytes were collected from 54 ICSI cycles. Read More

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Is neutral genetic diversity related to quantitative variation in semen traits in bulls?

Reprod Domest Anim 2021 Jul 20. Epub 2021 Jul 20.

Germplasm and Reproductive Technology Laboratory, Department of Animal Production & Technology, Chinhoyi University of Technology, Private Bag, 7724, Chinhoyi, Zimbabwe.

Conservation decisions based on neutral genetic diversity have been observed to promote retention of useful quantitative variation in biological populations. An experiment was undertaken to determine the association between microsatellite marker polymorphisms and phenotypic variation in semen production and cryosurvival traits in bulls. Thirty-five ejaculates were collected from ten bulls of two breeds and evaluated before and after cryopreservation for several semen traits. Read More

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Research Note: The effect of crude protein and calcium intake on fertility of male broiler breeders.

Poult Sci 2021 May 27;100(9):101284. Epub 2021 May 27.

Animal and Poultry Science, School of Agricultural, Earth and Environmental Sciences, University of KwaZulu-Natal, P. Bag X54001, Durban, 4000, South Africa.

This experiment investigated the effect of excess crude protein and/or calcium on male broiler breeder fertility. Forty-eight broiler breeder males, from a group of 60 that consistently produced a semen sample were allocated to one of 4 dietary treatments, formulated to provide either the male or female recommendation for crude protein and calcium, or their combinations. Birds were provided a constant daily feed allocation. Read More

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Assessing the clinical value of the Kruger strict morphology criteria over the World Health Organization fourth edition criteria.

F S Rep 2021 Jun 19;2(2):176-180. Epub 2021 Apr 19.

Center for Reproductive Medicine and Surgery, Institute for Reproductive Medicine and Department of Urology, Weill Cornell Medicine, New York, New York.

Objective: To assess if the newer Kruger strict morphology (WHO5; normal ≥4%) adds any clinical value beyond the criteria of the World Health Organization fourth edition (WHO4; normal ≥14%).

Design: Retrospective study.

Setting: Tertiary hospital. Read More

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Ameliorative role of trans-ferulic acid on induced oxidative toxicity of rooster semen by β-cyfluthrin during low temperature liquid storage.

Poult Sci 2021 Jun 9;100(9):101308. Epub 2021 Jun 9.

Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

Current study was designed to evaluate the effects of β-cyfluthrin, as a toxicant substance, and trans-ferulic acid (trans-FA), as a protective agent, on different parameters of rooster semen upon liquid storage. For this purpose, semen samples of roosters (Ross 308, n = 10, 32-wk-old) were collected twice a week. Good quality samples (≥70% progressive motion) were diluted, pooled and then divided for the purposes of the study. Read More

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Impaired semen parameters in patients with confirmed SARS-CoV-2 infection: A prospective cohort study.

Andrologia 2021 Jul 15:e14157. Epub 2021 Jul 15.

Department of Urology, Istanbul Training and Research Hospital, Istanbul, Turkey.

In this prospective study, we investigated the impact of SARS-CoV-2 infection on semen parameters in a cohort of men who had recently recovered from COVID-19. A total of 24 men who had recently recovered from mild COVID-19 were included in the study. Their semen parameters were normal before COVID-19 according to the World Health Organization 2010 reference values. Read More

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Does COVID-19 Worsen the Semen Parameters? Early Results of a Tertiary Healthcare Center.

Urol Int 2021 Jul 15:1-6. Epub 2021 Jul 15.

Department of Urology, Ankara State Hospital, Ankara, Turkey.

Introduction: The coronavirus disease 2019 (COVID-19) is a global pandemic which may affect multiple organs and systems including testes and disrupt the gonadal functions. The current study aimed to evaluate the effect of COVID-19 on the semen parameters and sex-related hormone levels in infertile men.

Methods: The study included 21 patients who were evaluated in Ankara City Hospital, Andrology Clinic, for male infertility and have had the diagnosis of COVID-19. Read More

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Response of rams to electroejaculation following the administration of oxytocin and cloprostenol with or without GnRH.

Theriogenology 2021 Jun 30;173:32-36. Epub 2021 Jun 30.

Faculdade de Veterinária, Universidade Federal Fluminense, Niterói, Rio de Janeiro, 24230-340, Brazil. Electronic address:

The present study aims to evaluate the effect of administering prostaglandin (250 μg cloprostenol) and oxytocin (10 UI) or a GnRH agonist (4.2 μg buserelin acetate) on rams' physiological responses to electroejaculation and the ejaculate's characteristics. The study was performed with a 2 × 2 factorial arrangement, according to whether it used oxytocin and prostaglandin (OXPGF) or GnRH. Read More

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Supplementation of Beltsville extender with quercetin improves the quality of frozen-thawed rooster semen.

Br Poult Sci 2021 Jul 14. Epub 2021 Jul 14.

Department of Poultry Science, Faculty of Agriculture, University of Tarbiat Modares, Tehran, Iran.

Excessive reactive oxygen species (ROS) production during the sperm freeze-thawing process leads to membrane lipid peroxidation, DNA damage, and motility loss.This study examined the effect of supplementation of Beltsville poultry semen extender with different concentrations of quercetin (and antioxidant) on the cryopreservation of rooster sperm.Semen samples were collected from six Ross broiler breeders abdominal massage twice a week for four weeks (eight replicates), and was divided into five equal aliquots to be diluted in Beltsville extender that contained different concentrations of quercetin: 0, 5, 10, 15, and 20 mM. Read More

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Membrane lipid replacement with nano-micelles in human sperm cryopreservation improves post-thaw function and acrosome protein integrity.

Reprod Biomed Online 2021 Aug 15;43(2):257-268. Epub 2021 May 15.

Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Banihashem St Tehran 16635-148, Iran. Electronic address:

Research Question: Membrane lipid replacement (MLR) of oxidized membrane lipids can restore sperm cellular membrane functionality and help improve surface protein stability during cryopreservation. What are the effects of MLR with nano-micelles made from a glycerophospholipid (GPL) mixture and cholesterol-loaded cyclodextrin (CLC), on the cryosurvival and expression of acrosome-related proteins in thawed human spermatozoa?

Design: Twenty samples were used to determine the optimum level of nano-micelles by incubation of semen with different concentrations of GPL (0.1 and 1%) and CLC (1 and 2 mg/ml) (including GPL-0. Read More

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Effects of different ultrastructures of lecithin on cryosurvival of goat spermatozoa.

Andrologia 2021 Jul 13:e14183. Epub 2021 Jul 13.

Department of Animal Science, Campus of Agriculture & Natural Resources, University of Tehran, Karaj, Iran.

This study was to evaluate the effects of two different ultrastructures of lecithin including nanoparticles (NPE mostly nanomicelles) and lecithin nanoliposome (NLE) with egg yolk extender (EYE) on goat sperm cryopreservation. Semen samples were collected from 6 goats, then pooled, diluted and then frozen. Motility and motion parameters, plasma membrane integrity and functionality, morphology, apoptosis status (Annexin V-PI), acrosome integrity, DNA fragmentation and in vitro fertilisation were assessed. Read More

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Comprehensive functional analysis reveals that acrosome integrity and viability are key variables distinguishing artificial insemination bulls of varying fertility.

J Dairy Sci 2021 Jul 9. Epub 2021 Jul 9.

Laboratory of Animal Reproduction, Department of Biological Sciences, Biomaterials Research Cluster, Bernal Institute, Faculty of Science and Engineering, University of Limerick, Limerick, V94 T9PX, Ireland.

In vitro methods of assessing bull semen quality in artificial insemination (AI) centers are unable to consistently detect individuals of lower fertility, and attempts to reliably predict bull fertility are still ongoing. This highlights the need to identify robust biomarkers that can be readily measured in a practical setting and used to improve current predictions of bull fertility. In this study, we comprehensively analyzed a range of functional, morphological, and intracellular attributes in cryopreserved spermatozoa from a selected cohort of Holstein Friesian AI bulls classified as having either high or low fertility (n = 10 of each fertility phenotype; difference of 11. Read More

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Can detomidine replace medetomidine for pharmacological semen collection in domestic cats?

Anim Reprod 2021 2;18(2):e20210017. Epub 2021 Jul 2.

Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.

Among the different methods used for semen collection from domestic cats, the pharmacological collection by urethral catheterization becomes disruptive. Medetomidine is the elected α-adrenoceptor agonist for that, but in several countries, it is not commercially available. This study aimed to evaluate the efficacy of detomidine compared to medetomidine in collecting semen by urethral catheterization in domestic cats. Read More

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Successful blastocyst production by intracytoplasmic injection of sperm after in vitro maturation of follicular oocytes obtained from immature female squirrel monkeys (Saimiri boliviensis).

J Reprod Dev 2021 Jul 9. Epub 2021 Jul 9.

Graduate School of Agricultural Science, Utsunomiya University, Tochigi 321-8505, Japan.

Advanced reproductive technologies are being applied for the propagation of squirrel monkeys, to ensure their preservation as a genetic resource and the effective use of their gametes in the future. In the present study, oocytes and spermatozoa were collected from live squirrel monkeys, following which piezo intracytoplasmic sperm injection (ICSI) was performed using these gametes. Follicular development was induced by administering equine chorionic gonadotropin (eCG) containing inhibin antiserum to an immature squirrel monkey female. Read More

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The effect of sperm DNA fragmentation on intracytoplasmic sperm injection outcome.

Andrologia 2021 Jul 11:e14180. Epub 2021 Jul 11.

Urology Department, Hamad Medical Corporation, Doha, Qatar.

Our study objective was to assess the effect of various sperm DNA fragmentation levels on clinical intracytoplasmic sperm injection outcome. This retrospective study included 392 patients who underwent ICSI and performed sperm DNA fragmentation testing before the procedure. Based on sperm DNA fragmentation cut-off values, the patients were differentiated into 3 groups as <20%, 20%-30% and >30%. Read More

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Urine, feces, and blood contamination of frozen and fresh tambaqui (Colossoma macropomum Cuvier, 1818) sperm.

Cryobiology 2021 Jul 7. Epub 2021 Jul 7.

Animal Science Research Program, Federal University of Rio Grande do Sul. Av. Bento Gonçalves, 7712, Porto Alegre - 91540-000 - Rio Grande do Sul, Brasil. Electronic address:

Contamination of fish milt during collection can have an important effect on the quality of fresh and frozen samples. The aim of this study was to evaluate the effects of biological contaminants (urine, feces, and blood) on the sperm of Colossoma macropomum. After hormonal induction, contaminated and contaminant-free milt samples from thirteen males (6. Read More

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The effects of varying concentrations of glutathione and trehalose in improving microscopic and oxidative stress parameters in Turkey semen during liquid storage at 5 °C.

Cryobiology 2021 Aug 8;101:12-19. Epub 2021 Jul 8.

Department of Animal Science, Faculty of Agricultural Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.

Since turkey reproduction is mainly through artificial insemination, short-term preservation of turkey semen is one of the most important issues in turkey reproduction management. The present study investigates the effects of glutathione (GSH) and trehalose on lipid peroxidation degree and turkey semen quality while being stored at 5 °C for 72 h. To this end, semen samples were collected from 20 turkeys with a weekly frequency for 12 weeks. Read More

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