Reprod Domest Anim 2021 Aug 2. Epub 2021 Aug 2.
Department of Veterinary Surgery and Animal Reproduction, FMVZ, São Paulo State University (UNESP), Botucatu, Brazil.
This study aimed to describe successful cryopreservation of sperm from maned wolves (Chrysocyon brachyurus). Three ejaculates from 2 maned wolves were collected by digital manipulation of the penis and evaluated subjectively, centrifuged, and frozen in BotuCrio (Botupharma, Botucatu, Brazil) or TRIS - yolk egg extender. Spermatozoa were thawed at 37 ºC/30s or 70 ºC/4s and evaluated for kinetics, morphology, plasma and acrosome membrane integrity, mitochondrial potential, hydrogen peroxide, superoxide anion, and lipid peroxidation. Read More