J Phys Chem B 2021 04 31;125(14):3556-3565. Epub 2021 Mar 31.
Chemistry Department, Saint Louis University, 3501 Laclede Avenue, Saint Louis 63103, Missouri, United States.
The UV-vis absorption, Raman imaging, and resonance Raman (rR) spectroscopy methods were employed to study cyanohemoglobin (HbCN) adducts inside living functional red blood cells (RBCs). The cyanide ligands are especially optically sensitive probes of the active site environment of heme proteins. The rR studies of HbCN and its isotopic analogues (CN, CN, and CN), as well as a careful deconvolution of spectral data, revealed that the ν(Fe-CN) stretching, δ(Fe-CN) bending, and ν(C≡N) stretching modes occur at 454, 382, and 2123 cm, respectively. Read More