J Pharm Biomed Anal 2022 Jan 22;207:114435. Epub 2021 Oct 22.
School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, PR China; Key Laboratory of Digitalization Quality Evaluation of Chinese Materia Medica of SATCM, Guangzhou 510006, PR China; Research Center for Quality Engineering & Technology of Chinese Materia Medica in Guangdong Universities, Guangzhou 510006, PR China; Research Center for Quality Engineering & Technology of Chinese Materia Medica of Guangdong Province, Guangzhou 510006, PR China. Electronic address:
Objective: To establish a rapid and comprehensive method for the quality evaluation of Plantaginis Semen (PS) by using near-infrared spectroscopy combined with chemometrics to determine the content of geniposidic acid (GEA), verbascoside (VE), isoverbascoside (IVE) and total flavonoids (TF) in PS, as well as the antioxidant activity (AOA).
Methods: The content of GEA, VE and IVE in PS were determined by HPLC, the content of TF in PS was determined by UV-Vis spectrophotometry, and the AOA of PS was characterized by the DPPH, ABTS and FRAP, respectively. Then, the measured values of each item were used as reference values and were correlated with the near infrared spectra of PS. Read More