7,190 results match your criteria specific il-12

Latent tuberculosis co-infection is associated with heightened levels of humoral, cytokine and acute phase responses in seropositive SARS-CoV-2 infection.

J Infect 2021 Jul 27. Epub 2021 Jul 27.

ICMR-National Institute for Research in Tuberculosis-International Center for Excellence in Research, Chennai, India.

Objectives: Latent Tuberculosis infection (LTBI) is postulated to modulate immune responses and alter disease severity in SARS-CoV-2 co-infection. However, no data exist on the effect of LTBI on the immune responses in SARS-CoV-2 co-infected individuals.

Methods: We examined the SARS-CoV-2 specific antibody responses, plasma cytokines, chemokines, acute phase proteins and growth factor levels in LTBI positive and negative individuals with SARS-CoV-2 infection. Read More

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Chitosan Nanovaccines as Efficient Carrier Adjuvant System for IL-12 with Enhanced Protection Against HBV.

Int J Nanomedicine 2021 21;16:4913-4928. Epub 2021 Jul 21.

Institute of Immunopharmaceutical Sciences, School of Pharmaceutical Sciences, Shandong University, Jinan, People's Republic of China.

Purpose: Alum adjuvant in HBV prophylactic vaccines is poor in inducing cellular immunity with the inhibition of IL-12 secretion, and approximately 5-10% of immunised individuals fail to clear HBV upon infection. IL-12 plasmids (pIL-12) as adjuvants enhance significant humoral and cellular immune response in vaccines. However, finding a novel delivery system to protect pIL-12 from enzymatic degradation and achieve efficient delivery remains a major challenge. Read More

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High-parameter cytometry unmasks microglial cell spatio-temporal response kinetics in severe neuroinflammatory disease.

J Neuroinflammation 2021 Jul 26;18(1):166. Epub 2021 Jul 26.

Discipline of Pathology, Faculty of Medicine and Health, School of Medical Sciences, The University of Sydney, Sydney, Australia.

Background: Differentiating infiltrating myeloid cells from resident microglia in neuroinflammatory disease is challenging, because bone marrow-derived inflammatory monocytes infiltrating the inflamed brain adopt a 'microglia-like' phenotype. This precludes the accurate identification of either cell type without genetic manipulation, which is important to understand their temporal contribution to disease and inform effective intervention in its pathogenesis. During West Nile virus (WNV) encephalitis, widespread neuronal infection drives substantial CNS infiltration of inflammatory monocytes, causing severe immunopathology and/or death, but the role of microglia in this remains unclear. Read More

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Psoriasis as an adverse reaction to biologic agents beyond anti-TNF-α therapy.

Eur J Dermatol 2021 Jun;31(3):307-317

Joint Rheumatology Program, 1st Department of Propaedeutic Internal Medicine-Rheumatology Unit, National and Kapodistrian University of Athens, School of Medicine, Laikon General Hospital, Ag. Thoma 17, 11527, Athens, Greece.

New onset or exacerbation of pre-existing psoriasis after therapeutic TNF-α inhibition is a well-described phenomenon. Over the last two decades, similar cases of paradoxical psoriasis have been reported following the administration of other biologic agents. We aimed to review all published cases of induced or exacerbated psoriasis after biologic therapy other than anti-TNF-α agents in order to further elucidate the pathophysiology of this phenomenon. Read More

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Analysis of Circulating Immune Biomarkers by Race in Men with Metastatic Castration-Resistant Prostate Cancer Treated with Sipuleucel-T.

J Natl Cancer Inst 2021 Jul 24. Epub 2021 Jul 24.

Division of Hematology and Oncology, Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, New York, NY, USA.

Among racial subgroups, Black men have the highest prostate cancer-specific death rate, yet they also exhibit prolonged overall survival compared to White men when treated with standard therapies, including sipuleucel-T. Differential immune responses may play a role in these observations. We compared circulating immune markers from fifty-four men (18 Black and 36 White men) with metastatic castrate-resistant prostate cancer who received sipuleucel-T and were enrolled on an immune monitoring registry. Read More

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Cytokine/Chemokine Release Patterns and Transcriptomic Profiles of LPS/IFNγ-Activated Human Macrophages Differentiated with Heat-Killed , M-CSF, or GM-CSF.

Int J Mol Sci 2021 Jul 5;22(13). Epub 2021 Jul 5.

Department of Biomedical Sciences, Faculty of Medicine and Medical Sciences, University of Balamand, 33 Amioun, Al Kurah 100, Lebanon.

Macrophages (Mφs) are instrumental regulators of the immune response whereby they acquire diverse functional phenotypes following their exposure to microenvironmental cues that govern their differentiation from monocytes and their activation. The complexity and diversity of the mycobacterial cell wall have empowered mycobacteria with potent immunomodulatory capacities. A heat-killed (HK) whole-cell preparation of () has shown promise as an adjunctive immunotherapeutic agent for the treatment of cancer. Read More

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Type 1 conventional dendritic cells and interferons are required for spontaneous CD4 and CD8 T-cell protective responses to breast cancer.

Clin Transl Immunology 2021 14;10(7):e1305. Epub 2021 Jul 14.

Centre d'Immunologie de Marseille-Luminy Turing Center for Living Systems CNRS INSERM Aix Marseille Univ Marseille France.

Objectives: To better understand how immune responses may be harnessed against breast cancer, we investigated which immune cell types and signalling pathways are required for spontaneous control of a mouse model of mammary adenocarcinoma.

Methods: The NOP23 mammary adenocarcinoma cell line expressing epitopes derived from the ovalbumin model antigen is spontaneously controlled when orthotopically engrafted in syngeneic C57BL/6 mice. We combined this breast cancer model with antibody-mediated depletion of lymphocytes and with mutant mice affected in interferon (IFN) or type 1 conventional dendritic cell (cDC1) responses. Read More

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Systems biology informed neural networks (SBINN) predict response and novel combinations for PD-1 checkpoint blockade.

Commun Biol 2021 Jul 15;4(1):877. Epub 2021 Jul 15.

Department of Applied Mathematics, University of Waterloo, Waterloo, ON, Canada.

Anti-PD-1 immunotherapy has recently shown tremendous success for the treatment of several aggressive cancers. However, variability and unpredictability in treatment outcome have been observed, and are thought to be driven by patient-specific biology and interactions of the patient's immune system with the tumor. Here we develop an integrative systems biology and machine learning approach, built around clinical data, to predict patient response to anti-PD-1 immunotherapy and to improve the response rate. Read More

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An updated review on Mendelian susceptibility to mycobacterial diseases - a silver jubilee celebration of its first genetic diagnosis.

Expert Rev Clin Immunol 2021 Jul 14. Epub 2021 Jul 14.

Allergy Immunology Unit, Department of Pediatrics, Advanced Pediatrics Centre, Post Graduate Institute of Medical Education and Research, Chandigarh 160012, India.

Introduction: Mendelian susceptibility to mycobacterial diseases (MSMD), a group of at least 18 different genetic disorders, encompasses a specific class of inborn errors of immunity that result in predilection to infection with mycobacteria including the weakly virulent strains. Primarily, these consist of defects in the IFN-γ-IL-12/23 circuit that is crucial for immunity against intracellular microorganisms. Although the first genetic etiology of MSMD was discovered in 1996, molecular diagnosis of MSMD in resource-constrained settings may remain far-fetched. Read More

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Retardant effect of dihydroartemisinin on ulcerative colitis in a JAK2/STAT3-dependent manner.

Acta Biochim Biophys Sin (Shanghai) 2021 Jul 14. Epub 2021 Jul 14.

Department of General Surgery, General Surgery Institute of Yangzhou, Northern Jiangsu People's Hospital, Clinical Medical College, Yangzhou University, Yangzhou 225001, China.

Dihydroartemisinin (DHA) is a semi-synthetic derivative and the main active metabolite of artemisinin. The purpose of this study was to investigate the effect of DHA on the ulcerative colitis (UC) in both in vivo and in vitro models. Weight, survival rate, colon length, and Disease Activity Index score were used to evaluate the severity of colitis. Read More

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High concentrations of environmental ammonia induced changes in large-scale loach () immunity.

Ecol Evol 2021 Jul 28;11(13):8614-8622. Epub 2021 May 28.

College of Animal Science and Technology Anhui Agricultural University Hefei China.

High concentrations of environmental ammonia can cause reduced immunity and death in fish, causing enormous economic losses. Air-breathing fish usually have a high ammonia tolerance and are very suitable for high-density fish farming. However, research on the effects of environmental ammonia on air-breathing fish immunity is lacking. Read More

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TYK2 as a therapeutic target in the treatment of autoimmune and inflammatory diseases.

Immunotherapy 2021 Jul 8. Epub 2021 Jul 8.

Department of Dermatology, Military Institute of Medicine, Warsaw, Poland.

JAKs are intracellular protein tyrosine kinases that, through activation of STATs, are responsible for signal transduction pathways that regulate cellular responses to numerous cytokines, growth factors and hormones in many different cells. JAK-STAT signaling plays a key role in regulating immune function, and cytokines - such as IL-23, IL-12 and type I interferons - are central to the pathogenesis of autoimmune diseases, including psoriasis, inflammatory bowel disease and systemic lupus erythematosus. Here the authors review the evidence for targeting TYK2 as a more specific approach to treating these conditions. Read More

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Enhancement of CD4 T Cell Function as a Strategy for Improving Antibiotic Therapy Efficacy in Tuberculosis: Does It Work?

Front Cell Infect Microbiol 2021 21;11:672527. Epub 2021 Jun 21.

Immunobiology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, United States.

Tuberculosis (TB), caused by (Mtb) remains a major public health problem worldwide due in part to the lack of an effective vaccine and to the lengthy course of antibiotic treatment required for successful cure. Combined immuno/chemotherapeutic intervention represents a major strategy for developing more effective therapies against this important pathogen. Because of the major role of CD4 T cells in containing Mtb infection, augmentation of bacterial specific CD4 T cell responses has been considered as an approach in achieving this aim. Read More

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Human IL-15 Inhibits NK Cells Specific for Human NK-92 Cells.

Anticancer Res 2021 Jul;41(7):3281-3285

Cell Biology Unit, Faculty of Natural Sciences and Engineering, Åbo Akademi University, Turku, Finland

Background/aim: Recent studies have indicated that natural killer (NK) cells present in peripheral blood mononuclear cells (PBMCs) might be responsible for the somewhat poor outcome of clinical trials conducted with the NK cell line NK-92, as well as chimeric antigen receptor-modified NK-92 cells against leukemias and lymphomas. These NK cells and how their cytotoxic profiles can be altered by some common gamma chain receptor-dependent cytokines or by removal of CD4 cells have been addressed herein.

Materials And Methods: A time-resolved fluorometric assay using 2. Read More

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Phase 1 study of safety, tolerability and immunogenicity of the human telomerase (hTERT)-encoded DNA plasmids INO-1400 and INO-1401 with or without IL-12 DNA plasmid INO-9012 in adult patients with solid tumors.

J Immunother Cancer 2021 Jul;9(7)

Inovio Pharmaceuticals, Plymouth Meeting, Pennsylvania, USA.

Background: Human telomerase reverse transcriptase (hTERT) is frequently classified as a 'universal' tumor associated antigen due to its expression in a vast number of cancers. We evaluated plasmid DNA-encoded hTERT as an immunotherapy across nine cancer types.

Methods: A phase 1 clinical trial was conducted in adult patients with no evidence of disease following definitive surgery and standard therapy, who were at high risk of relapse. Read More

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Concomitant experimental coinfection by Plasmodium berghei NK65-NY and Ascaris suum downregulates the Ascaris-specific immune response and potentiates Ascaris-associated lung pathology.

Malar J 2021 Jul 1;20(1):296. Epub 2021 Jul 1.

Laboratory of Immunology and Genomics of Parasites, Institute of Biological Sciences, Department of Parasitology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

Background: Ascariasis and malaria are highly prevalent parasitic diseases in tropical regions and often have overlapping endemic areas, contributing to high morbidity and mortality rates in areas with poor sanitary conditions. Several studies have previously aimed to correlate the effects of Ascaris-Plasmodium coinfections but have obtained contradictory and inconclusive results. Therefore, the present study aimed to investigate parasitological and immunopathological aspects of the lung during murine experimental concomitant coinfection by Plasmodium berghei and Ascaris suum during larvae ascariasis. Read More

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CD44 Sorted Cells Have an Augmented Potential for Proliferation, Epithelial-Mesenchymal Transition, Stemness, and a Predominantly Inflammatory Cytokine and Angiogenic Secretome.

Curr Issues Mol Biol 2021 Jun 21;43(1):423-433. Epub 2021 Jun 21.

Department of Maxillofacial Surgery and Diagnostic Sciences, Division of Oral Pathology, College of Dentistry, Jazan University, Jazan 45142, Saudi Arabia.

Cancer stem cells (CSCs) have garnered attention with their potential for early diagnosis and prognosis of oral squamous cell carcinoma (OSCC). It is still indistinct whether CSCs are recognized with a specific set of characteristics. The present study aimed to assess the association of CD44 with stemness-related, Epithelial Mesenchymal Transition EMT-related genes and the secretome of the CSCs. Read More

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Interleukin-35 is a critical regulator of immunity during helminth infections associated with multiple sclerosis.

Immunology 2021 Jul 1. Epub 2021 Jul 1.

Neuroimmunology Unit, Department of Neuroscience, Hospital Alemán, Buenos Aires, Argentina.

Multiple sclerosis (MS) is currently thought to arise by interactions between genetic susceptibility and environmental factors. Infections in general trigger autoimmune responses causing clinical manifestations of disease. However, as a result of regulatory T (Treg)- and regulatory B (Breg)-cell induction, helminth infections tend to dampen disease activity. Read More

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Diospyros Peregrina Fruit Preparation Mediated Immunomodulation of Lymphocytes Isolated From The Blood of Breast Cancer Patients.

Iran J Immunol 2021 Jun;18(2):111-115

SRM Research Institute and Department of Biotechnology, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu 603203, India.

Background: Breast cancer is an uncontrolled growth of epithelial cells. The loss of Breast Cancer gene1 (BRCA1) activity due to mutation or down-regulation of gene expression promotes tumorigenesis and increases the risk of breast cancer.

Objectives: We aimed to pulsate lymphocytes of breast cancer patients and normal individuals, using Diospyros peregrina fruit preparation (DFP) to study the cancer-protective immunity, and the signal transduction processes involved with it. Read More

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Efficient Immunization of BALB/c Mice against Pathogenic Brucella melitensis and : Comparing Cell-Mediated and Protective Immune Responses Elicited by pCDNA3.1 and pVAX1 DNA Vaccines Coding for Omp31 of Brucella melitensis.

Iran J Biotechnol 2021 Jan 1;19(1):e2618. Epub 2021 Jan 1.

Department of Cardiology, Charité Medical University of Berlin, Berlin, Germany.

Background: spp. are intracellular pathogens, therefore cell-mediated immunity is the main response to inhibit survival and growth of the bacteria in vertebrate host.

Objective: Many eukaryotic plasmid vectors are being used in setting up DNA vaccines which may show different efficiencies in same conditions. Read More

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January 2021

Adverse Events Associated With Anti-IL-23 Agents: Clinical Evidence and Possible Mechanisms.

Front Immunol 2021 11;12:670398. Epub 2021 Jun 11.

Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Background: Anti-interleukin (IL)-23 agents are widely used for autoimmune disease treatment; however, the safety and risks of specific symptoms have not been systematically assessed.

Objectives: The aim of this study was to summarize the characteristics and mechanisms of occurrence of five immunological and non-immunological adverse events caused by different anti-IL-23 agents.

Methods: The Cochrane Library, EMBASE, PubMed, and Web of Science databases were searched for eligible randomized clinical trials published from inception through May 1, 2020. Read More

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Anti-interferon-γ autoantibody-associated immunodeficiency.

Curr Opin Immunol 2021 Jun 24;72:206-214. Epub 2021 Jun 24.

Laboratory of Human Immunology and Infectious Diseases, Graduate Institute of Clinical Medical Sciences, Chang Gung University, Taoyuan City 33302, Taiwan; Department of Nephrology, Chang Gung Memorial Hospital, Taoyuan City 33305, Taiwan. Electronic address:

Anticytokine autoantibodies are an emerging disease etiology, through the disturbance of physiological functions of cognate cytokines. Anti-interferon (IFN)-γ autoantibodies (AIGAs) were first identified in patients with severe mycobacterial infections, and were considered to be an autoimmune phenocopy of inborn genetic errors of the IL-12/IFN-γ axis. More than 600 reported cases, most originating from Southeast Asia, have been diagnosed over the last decade. Read More

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Diagnosis of Chikungunya virus in febrile patients from a malaria holoendemic area.

Int J Infect Dis 2021 Jun 23. Epub 2021 Jun 23.

FondationCongolaise pour la RechercheMédicale (FCRM), Brazzaville, Republic of Congo.; Faculty of Sciences and Technology, University Marien Ngouabi, Brazzaville, Republic of Congo.; Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany.. Electronic address:

Introduction: Accurate diagnosis of chikungunya is essential for effective disease management and surveillance. In a cohort of febrile Congolese patients, we evaluated available diagnostic methods widely used in chikungunya diagnosis. In addition, plasma cytokines were quantified in chikungunya patients and in patients coinfected with malaria compared with healthy controls. Read More

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Adjuvant-free peptide vaccine targeting Clec9a on dendritic cells can induce robust antitumor immune response through Syk/IL-21 axis.

Theranostics 2021 24;11(15):7308-7321. Epub 2021 May 24.

School of Life Sciences, Zhengzhou University, Zhengzhou 450001, China.

Dendritic cells (DCs) can process the antigens of cancer vaccine and thus stimulate the CD8 T cells to recognize and kill the tumor cells that express these antigens. However, lack of promising carriers for presenting the antigens to DCs is one of the main barriers to the development of clinically effective cancer vaccines. Another limitation is the risk of inflammatory side effects induced by the adjuvants. Read More

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Convergence of cytokine dysregulation and antibody deficiency in common variable immunodeficiency with inflammatory complications.

J Allergy Clin Immunol 2021 Jun 17. Epub 2021 Jun 17.

Pulmonary Center and Section of Pulmonary, Allergy, Sleep & Critical Care, Department of Medicine, Boston University School of Medicine, Boston, Mass. Electronic address:

Background: Noninfectious complications are the greatest cause of morbidity and mortality in common variable immunodeficiency (CVID), but their pathogenesis remains poorly defined.

Objective: Using high-throughput approaches, we aimed to identify, correlate, and determine the significance of immunologic features of CVID with noninfectious complications (CVIDc).

Methods: We simultaneously applied proteomics, RNA sequencing, and mass cytometry to a large cohort with primary antibody deficiency. Read More

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Effects of therapeutic vaccination on the control of SIV in rhesus macaques with variable responsiveness to antiretroviral drugs.

PLoS One 2021 17;16(6):e0253265. Epub 2021 Jun 17.

Department of Microbiology, University of Washington, Seattle, Washington, United States of America.

A therapeutic vaccine that induces lasting control of HIV infection could eliminate the need for lifelong adherence to antiretroviral therapy. This study investigated a therapeutic DNA vaccine delivered with a single adjuvant or a novel combination of adjuvants to augment T cell immunity in the blood and gut-associated lymphoid tissue in SIV-infected rhesus macaques. Animals that received DNA vaccines expressing SIV proteins, combined with plasmids expressing adjuvants designed to increase peripheral and mucosal T cell responses, including the catalytic subunit of the E. Read More

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STAT4 is expressed in neutrophils and promotes antimicrobial immunity.

JCI Insight 2021 Jul 22;6(14). Epub 2021 Jul 22.

Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, Norfolk, Virginia, USA.

Signal transducer and activator of transcription 4 (STAT4) is expressed in hematopoietic cells and plays a key role in the differentiation of T helper 1 cells. Although STAT4 is required for immunity to intracellular pathogens, the T cell-independent protective mechanisms of STAT4 are not clearly defined. In this report, we demonstrate that STAT4-deficient mice were acutely sensitive to methicillin-resistant Staphylococcus aureus (MRSA) infection. Read More

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The inhibition of NFкB signaling and inflammatory response as a strategy for blunting bile acid-induced hepatic and renal toxicity.

Toxicol Lett 2021 Oct 2;349:12-29. Epub 2021 Jun 2.

Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address:

The cholestatic liver injury could occur in response to a variety of diseases or xenobiotics. Although cholestasis primarily affects liver function, it has been well-known that other organs such as the kidney could be influenced in cholestatic patients. Severe cholestasis could lead to tissue fibrosis and organ failure. Read More

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October 2021

Interleukin-27 and Its Diverse Effects on Bacterial Infections.

Front Immunol 2021 17;12:678515. Epub 2021 May 17.

Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY, United States.

Innate and adaptive immune responses against pathogens are known to be carefully orchestrated by specific cytokines that initiate and down regulate immune cell functions from the initial infection through tissue repair and homeostasis. However, some cytokines, including interleukin-27, are expressed at multiple phases of the infection, such that their pro and anti-inflammatory functions have been difficult to interpret. As elucidation of specific cytokine functions throughout infection is central to our understanding of protective susceptible immunity and return to homeostasis prolonged inflammation leading to septic shock, here we review the literature on IL-27 signaling and the various functions of this heterodimeric ligand member of the IL-12 cytokine family. Read More

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Neutrophil Cells Are Essential for The Efficacy of a Therapeutic Vaccine against Paracoccidioidomycosis.

J Fungi (Basel) 2021 May 26;7(6). Epub 2021 May 26.

Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508-000, Brazil.

Paracoccidioidomycosis (PCM), caused by the species, is a systemic disease endemic in several Latin American countries, mainly in Brazil, Colombia, Argentina, and Venezuela. Current treatment approaches are challenging as they require prolonged durations of antifungal drugs that have potential toxicities, and despite antifungals, relapses are common. Hence, new therapeutic approaches, such as vaccines, are being investigated. Read More

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