Heliyon 2021 May 25;7(5):e07084. Epub 2021 May 25.
Sofia University St. "Kliment Ohridski", Faculty of Biology, Department of General and Industrial Microbiology, 8 Dragan Tsankov Blvd, Sofia 1000, Bulgaria.
The urgent need of research of new approaches to control bacterial disease on economical important crops, focuses our attention on bacteriophages as alternative biocontrol agents. Thus, the purpose of this paper is to present the isolation and initial characterization of three bacteriophages (SfXv124t/1, 2 and 3) isolated from rhizosphere soil of a healthy tomato plant in Bulgaria that are capable to lyse three phytopathogenic bacteria. The initial characterization includes determination of: their host range, plaque morphology, optimal storage temperature of pure phage lysates, their sensitivity to UV light, thermal inactivation, optimal multiplicity of infection (MOI) and virion morphology. Read More