27,646 results match your criteria species primers


β-lactamases ) and class C β-lactamases gene frequency in isolated from various clinical specimens in Khartoum State, Sudan: a cross sectional study.

F1000Res 2020 27;9:774. Epub 2020 Jul 27.

Biochemistry Department, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.

is a pathogenic bacterium, causing nosocomial infections with intrinsic and acquired resistance mechanisms to a large group of antibiotics, including β-lactams. This study aimed to determine the susceptibility pattern to selected antibiotics and to index the first reported β-lactamases gene (extended spectrum β-lactamases (ESBLs) genes and class C β-lactamases genes) frequency in in Khartoum State, Sudan. 121 clinical isolates from various clinical specimens were used in this cross-sectional study conducted in Khartoum State. Read More

View Article and Full-Text PDF

DNA polymerase D temporarily connects primase to the CMG-like helicase before interacting with proliferating cell nuclear antigen.

Nucleic Acids Res 2021 Apr 13. Epub 2021 Apr 13.

Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan.

The eukaryotic replisome is comprised of three family-B DNA polymerases (Polα, δ and ϵ). Polα forms a stable complex with primase to synthesize short RNA-DNA primers, which are subsequently elongated by Polδ and Polϵ in concert with proliferating cell nuclear antigen (PCNA). In some species of archaea, family-D DNA polymerase (PolD) is the only DNA polymerase essential for cell viability, raising the question of how it alone conducts the bulk of DNA synthesis. Read More

View Article and Full-Text PDF

Candidatus Dirofilaria Hongkongensis Infections in Humans During 2005 to 2020, in Kerala, India.

Am J Trop Med Hyg 2021 Apr 12. Epub 2021 Apr 12.

3Center for Nanoscience and Molecular Medicine, Amrita Institute of Medical Sciences and Research Center, Amrita University, Cochin, Kerala 682041, India.

We tried to determine the epidemiology and species of human dirofilariasis observed at two tertiary care hospitals in Kerala. We searched the hospital database to identify cases of dirofilariosis from January 2005 to March 2020. Along with human isolates, one dog Dirofilaria isolate was also subjected to PCR and sequencing of pan filarial primers cytochrome oxidase subunits 1 and 12S rDNA. Read More

View Article and Full-Text PDF

Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methods.

Clin Oral Investig 2021 Apr 12. Epub 2021 Apr 12.

Department of Restorative Dentistry, Division of Endodontics, Piracicaba Dental School, State University of Campinas - UNICAMP, Av. Limeira 901. Bairro Areao, Piracicaba, SP, Brazil.

Objective: Fusobacterium nucleatum is an important oral pathogen involved in endodontic infections. This study aimed to assess the frequency of Fusobacterium nucleatum in primary and secondary endodontic infections and its associations with the clinical features in a Brazilian population by using both culture and nested PCR methods.

Methods: A total of 100 microbial samples from patients with primary (n=50) and secondary endodontic infections (n=50) were analyzed by using culture and nested PCR methods. Read More

View Article and Full-Text PDF

CAP Analysis of the Distribution of the Introduced Bemisia tabaci (Hemiptera: Aleyrodidae) Species Complex in Xinjiang, China and the Southerly Expansion of the Mediterranean Species.

J Insect Sci 2021 Mar;21(2)

Institute of Microbial Application, Xinjiang Academy of Agricultural Sciences, Xinjiang, China.

Bemisia tabaci (Gennadius) cryptic complex has invaded Xinjiang, China, since 1998. The distribution of Mediterranean (MED) and Middle East-Asia Minor 1 (MEAM1) B. tabaci substrains has been gradually identified due to the development of molecular technology. Read More

View Article and Full-Text PDF

Direct Detection and Identification of the Most Common Bacteria and Fungi Causing Otitis Externa by a Stepwise Multiplex PCR.

Front Cell Infect Microbiol 2021 25;11:644060. Epub 2021 Mar 25.

Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Background: Considering the importance of differential diagnosis of infectious otitis externa (OE), a stepwise PCR-based assay using universal and genus- or species-specific primers for the detection/identification of the most prevalent bacterial and fungal OE was developed and evaluated on the ear aspiration specimens of clinically suspected patients.

Methods And Materials: A total of 120 ear aspiration specimens with otomycosis suspicion were subjected to manual DNA extraction using phenol-chloroform extraction after tissue digestion with a lysis buffer. The multiplex PCR was initially performed using pan-fungal and bacterial homemade primers. Read More

View Article and Full-Text PDF

Pangenome analyses of LuxS-coding genes and enzymatic repertoires in cocoa-related lactic acid bacteria.

Genomics 2021 Apr 8. Epub 2021 Apr 8.

University of Verona - Department of Biotechnology, Verona, Italy.

Lactobacillaceae presents potential for interspecific Quorum Sensing (QS) in spontaneous cocoa fermentation, correlated with high abundance of luxS. Three Brazilian isolates from cocoa fermentation were characterized by Whole Genome Sequencing and luxS gene was surveyed in their genomes, in comparison with public databases. They were classified as Lactiplantibacillus plantarum, Limosilactobacillus fermentum and Pediococcus acidilactici. Read More

View Article and Full-Text PDF

Partial overlap of fungal communities associated with nettle and poplar roots when co-occurring at a trace metal contaminated site.

Sci Total Environ 2021 Mar 24;782:146692. Epub 2021 Mar 24.

Chrono-environnement UMR6249, CNRS, Université Bourgogne Franche-Comté, F-25000 Besançon, France; Université de Lorraine, Faculté des Sciences et Technologies, 54000 Nancy, France.

Stinging nettle (Urtica dioica L.) raises growing interest in phytomanagement because it commonly grows under poplar Short Rotation Coppices (SRC) set up at trace-metal (TM) contaminated sites and provides high-quality herbaceous fibres. The mycobiome of this non-mycorhizal plant and its capacity to adapt to TM-contaminated environments remains unknown. Read More

View Article and Full-Text PDF

First Report of Causing Garlic clove Rot in Russian Federation.

Plant Dis 2021 Apr 9. Epub 2021 Apr 9.

FSI Federal Research Centre Fundamentals of Biotechnology of the Russian Academy of Sciences, 442108, Laboratory of plant system biology , Moscow, Moscow, Russian Federation;

Garlic () is a widely consumed bulbous crop both worldwide and in Russia. About 200,000 tons of garlic is produced in Russia annually (https://rosstat.gov. Read More

View Article and Full-Text PDF

Co-occurrence of true truffle mycelia in Tuber magnatum fruiting sites.

Mycorrhiza 2021 Apr 9. Epub 2021 Apr 9.

Department of Agricultural and Food Sciences, University of Bologna, viale Fanin 44, 40127, Bologna, Italy.

Tuber magnatum (the white truffle) is the most precious species of the genus Tuber which comprises the hypogeous ectomycorrhizal species called "true truffle." Despite its high economic value, the knowledge on its ecology is scant, principally due to the difficulty to find its mycorrhizas in the soil. The possibility to detect its mycelium by DNA extracted from soil has given a new chance for studying this truffle species. Read More

View Article and Full-Text PDF

Occurrence, characteristics and PCR-based detection of causing soft rot of Chinese cabbage in China.

Plant Dis 2021 Apr 9. Epub 2021 Apr 9.

Beijing 100097Beijing , China, 100097;

Bacterial soft rot is an important disease of Chinese cabbage ( L. ssp. ) in China and many other countries. Read More

View Article and Full-Text PDF

First Report of Wilt of European hornbeam (Carpinus betulus L.) Caused by in China.

Plant Dis 2021 Apr 9. Epub 2021 Apr 9.

Nanjing Forestry University, 74584, College of Forestry, No.159, Longpan Road, Xuanwu District, Nanjing, China, 210037.

European hornbeam (Carpinus betulus L.) has been used as an important ornamental species for urban landscaping since the Italian Renaissance (Rocchi et al. 2010). Read More

View Article and Full-Text PDF

Development a hydrolysis probe-based quantitative PCR assay for the specific detection and quantification of .

Curr Med Mycol 2020 Sep;6(3):50-56

Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

Background And Purpose: is an emerging multidrug-resistant pathogen. The identification of this species with the conventional phenotypic or biochemical mycological methods may lead to misidentification. Molecular-based species-specific identification methods such as quantitative real-time polymerase chain reaction (qPCR) facilitate a more reliable identification of than mycological methods. Read More

View Article and Full-Text PDF
September 2020

Identification of species using direct PCR- sequencing on clinical samples from patients with pityriasis versicolor and seborrheic dermatitis.

Curr Med Mycol 2020 Sep;6(3):21-26

Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Background And Purpose: yeasts are lipophilic normal flora of the skin in humans and other warm-blooded vertebrates. This genus includes 18 species and is responsible for dermatological disorders, such as pityriasis versicolor, atopic dermatitis, seborrheic dermatitis, folliculitis, and dandruff. The aim of the present study was to identify the etiologic agents of infections among the patients referring to the Referral Dermatology Clinic of Al-Zahra Hospital, Isfahan, Iran, during 2018-2019. Read More

View Article and Full-Text PDF
September 2020

Molecular detection of hupB gene of Mycobacterium tuberculosis in young patients with acute lymphoblastic leukemia (ALL) on Chemotherapy.

Pak J Pharm Sci 2020 Sep;33(5(Special)):2471-2475

Department of Environmental Sciences, University College of Agriculture and Environmental Sciences, The Islamia University of Bahawalpur, Pakistan.

Cancer chemotherapy can lead to the mycobacterial infections. Tuberculosis has been reported a serious complication in leukemia patients who undergo chemotherapy. The study was focused to find mutations in hupB gene of M. Read More

View Article and Full-Text PDF
September 2020

Evaluation of V2 18S rDNA barcode marker and assessment of sample collection and DNA extraction methods for metabarcoding of autotrophic euglenids.

Environ Microbiol 2021 Apr 8. Epub 2021 Apr 8.

Institute of Evolutionary Biology, Faculty of Biology, Biological and Chemical Research Centre, University of Warsaw, ul. Żwirki i Wigury 101, PL-02-089, Warsaw, Poland.

Even though the interest in metabarcoding in environmental studies is growing, euglenids are still underrepresented in both sea and freshwater bodies researches. The reason for this situation could be the unsuitability of universal eukaryotic DNA barcodes and primers as well as the lack of a verified protocol, suitable to assess euglenid diversity. In this study, using specific primers for the V2 hypervariable region of 18S rDNA for metabarcoding resulted in obtaining a high fraction (85%) of euglenid reads and species-level identification of almost 90% of them. Read More

View Article and Full-Text PDF

First report of squash vein yellowing virus naturally infecting butternut squash () in Texas.

Plant Dis 2021 Apr 6. Epub 2021 Apr 6.

Texas A&M University, Department of Plant Pathology & Microbiology, 2401 E. Bus. Hwy. 83, Weslaco, Texas, United States, 78596.

Virus diseases are major constraints to the production of cucurbits in the Texas Lower Rio Grande Valley. In September 2020, a ~8.1 ha butternut squash () field in Hidalgo County, Texas, was observed with virus-like symptoms of vein yellowing, leaf curl, mosaic, and foliar chlorosis. Read More

View Article and Full-Text PDF

Development of LAMP assays using a novel target gene for specific detection of Pythium terrestris, Pythium spinosum, and "Candidatus Pythium huanghuaiense".

Plant Dis 2021 Apr 6. Epub 2021 Apr 6.

Nanjing Agricultural University, 70578, Department of Plant Pathology, Nanjing, Jiangsu, China;

Pythium terrestris, Pythium spinosum, and "Candidatus Pythium huanghuaiense" are closely related species and important pathogens of soybean that cause root rot. However, the sequences of commonly-used molecular markers, such as rDNA internal transcribed spacer 2 and cytochrome oxidase 1 gene, are similar among these species, making it difficult to design species-specific primers for loop-mediated isothermal amplification (LAMP) assays. The genome sequences of these species are also currently unavailable. Read More

View Article and Full-Text PDF

First Report of Curly Top of Caused by in the Columbia Basin of Washington State.

Plant Dis 2021 Apr 6. Epub 2021 Apr 6.

Washington State University, Moses Lake, United States;

Two fields of coriander ( L.) seed crops of proprietary cultivars were observed in the Columbia Basin of Washington in July 2020 with 40 and 90% incidence of plants showing stunting and leaf and stem discoloration, sometimes with mild leaf curl. Foliar discoloration ranged from yellow to red and purple. Read More

View Article and Full-Text PDF

Identification of Reticulitermes Subterranean Termites (Blattodea: Rhinotermitidae) in the Eastern United States Using Inter-Simple Sequence Repeats.

J Econ Entomol 2021 Apr 3. Epub 2021 Apr 3.

Department of Entomology, Texas A&M University, College Station, TX, USA.

In the eastern United States, there are nine species of subterranean termites in three genera: Reticulitermes (six species), Coptotermes (two species), and Prorhinotermes (one species). These species serve as important ecological players by decomposing cellulose material, and some are important structural pests. Many of these species are difficult to discriminate morphologically and require examining the reproductive or soldier castes, which can be difficult to collect. Read More

View Article and Full-Text PDF

High fluoride resistance and virulence profile of environmental Pseudomonas isolated from water sources.

Folia Microbiol (Praha) 2021 Apr 5. Epub 2021 Apr 5.

Department of Molecular Biology, School of Biological Sciences, Madurai Kamaraj University, Madurai-21, India.

In our previous study, all Pseudomonas strains THP6, THP41, and OHP5 were identified as fluoride-resistant bacteria isolated from Dindigul district, Tamilnadu, India. The selected strains exhibiting a high level of fluoride resistance was determined in Luria broth (LB) medium and LB agar plates. In a further effort, fluoride-resistant organisms were tested for hemolytic activity and showed β-hemolysis on blood agar plates. Read More

View Article and Full-Text PDF

Hemagglutinin-neuraminidase Sequence and Phylogenetic Analysis of Two Newcastle Disease Virus Isolated from Chickens in Iran.

Arch Razi Inst 2021 Mar 1;76(1):31-39. Epub 2021 Mar 1.

Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

Newcastle disease is a highly contagious viral infection affecting many species of birds that can spread fast between poultry houses and cause a heavy economic burden on the poultry industry all around the world. Fusion and hemagglutinin-neuraminidase (HN) protein are important in the pathogenesis of the Newcastle disease virus (NDV). The HN protein is a critical viral protein with multiple functions and plays a key role in the formation of the virulence of NDV. Read More

View Article and Full-Text PDF