2,573 results match your criteria soluble liposomal

Paracoccus denitrificans: a genetically tractable model system for studying respiratory complex I.

Sci Rep 2021 May 12;11(1):10143. Epub 2021 May 12.

The Medical Research Council Mitochondrial Biology Unit, University of Cambridge, The Keith Peters Building, Cambridge Biomedical Campus, Hills Road, Cambridge, CB2 0XY, UK.

Mitochondrial complex I (NADH:ubiquinone oxidoreductase) is a crucial metabolic enzyme that couples the free energy released from NADH oxidation and ubiquinone reduction to the translocation of four protons across the inner mitochondrial membrane, creating the proton motive force for ATP synthesis. The mechanism by which the energy is captured, and the mechanism and pathways of proton pumping, remain elusive despite recent advances in structural knowledge. Progress has been limited by a lack of model systems able to combine functional and structural analyses with targeted mutagenic interrogation throughout the entire complex. Read More

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Role of IL-36γ/IL-36R Signaling in Corneal Innate Defense Against Candida albicans Keratitis.

Invest Ophthalmol Vis Sci 2021 May;62(6):10

Departments of Ophthalmology and Anatomy and Cell Biology Wayne State University School of Medicine, Detroit, Michigan, United States.

Purpose: Interleukin (IL)-36 cytokines have been shown to play either beneficial or detrimental roles in the infection of mucosal tissues in a pathogen-dependent manner, but their involvement in fungal keratitis remains elusive. We herein investigated their expression and function in mediating corneal innate immunity against Candida albicans infection.

Methods: Gene expression in mouse corneas with or without C. Read More

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Nanoformulation and Evaluation of Oral Berberine-Loaded Liposomes.

Molecules 2021 Apr 29;26(9). Epub 2021 Apr 29.

Department of Pharmaceutics, Hanoi University of Pharmacy, 13-15 Le Thanh Tong Street, Hoan Kiem District, Hanoi 110403, Vietnam.

Berberine (BBR) is a poorly water-soluble quaternary isoquinoline alkaloid of plant origin with potential uses in the drug therapy of hypercholesterolemia. To tackle the limitations associated with the oral therapeutic use of BBR (such as a first-pass metabolism and poor absorption), BBR-loaded liposomes were fabricated by ethanol-injection and thin-film hydration methods. The size and size distribution, polydispersity index (PDI), solid-state properties, entrapment efficiency (EE) and in vitro drug release of liposomes were investigated. Read More

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Ruthenium(II)-curcumin liposome nanoparticles: Synthesis, characterization, and their effects against cervical cancer.

Colloids Surf B Biointerfaces 2021 Apr 19;204:111773. Epub 2021 Apr 19.

Department of Bio-nanotechnology, Gachon University, Gyeonggi-Do, Republic of Korea. Electronic address:

Ruthenium complexes have increased the scope for improvement in current cancer treatment by replacing platinum-based drugs. However, to reduce metal-associated toxicity, a biocompatible flavonoid, such as curcumin, is indispensable, as it offers uncompensated therapeutic benefits through formation of complexes. In this study, we synthesized metal-based flavonoid complexes using ruthenium(II) and curcumin by adopting a convenient reflux reaction, represented as Ru-Cur complexes. Read More

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The Comparison of In Vitro Photosensitizing Efficacy of Curcumin-Loaded Liposomes Following Photodynamic Therapy on Melanoma MUG-Mel2, Squamous Cell Carcinoma SCC-25, and Normal Keratinocyte HaCaT Cells.

Pharmaceuticals (Basel) 2021 Apr 17;14(4). Epub 2021 Apr 17.

Department of Pathology, Wroclaw Medical University, 50-367 Wroclaw, Poland.

The research focused on the investigation of curcumin encapsulated in hydrogenated soy phosphatidylcholine liposomes and its increased photoactive properties in photodynamic therapy (PDT). The goal of this study was two-fold: to emphasize the role of a natural photoactive plant-based derivative in the liposomal formulation as an easily bioavailable, alternative photosensitizer (PS) for the use in PDT of skin malignancies. Furthermore, the goal includes to prove the decreased cytotoxicity of phototoxic agents loaded in liposomes toward normal skin cells. Read More

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Solution and Membrane Interaction Dynamics of Fatty Acyl-CoA Synthetase FadD13.

Biochemistry 2021 Apr 29. Epub 2021 Apr 29.

Department of Biochemistry and Biophysics, Stockholm University, 106 91 Stockholm, Sweden.

The very-long-chain fatty acyl-CoA synthetase FadD13 from activates fatty acids for further use in mycobacterial lipid metabolism. FadD13 is a peripheral membrane protein, with both soluble and membrane-bound populations . The protein displays a distinct positively charged surface patch, suggested to be involved in membrane association. Read More

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Reconstitution of GABA, Glycine and Glutamate Transporters.

Neurochem Res 2021 Apr 27. Epub 2021 Apr 27.

Neurotransporter Group, Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, 0317, Oslo, Norway.

In contrast to water soluble enzymes which can be purified and studied while in solution, studies of solute carrier (transporter) proteins require both that the protein of interest is situated in a phospholipid membrane and that this membrane forms a closed compartment. An additional challenge to the study of transporter proteins has been that the transport depends on the transmembrane electrochemical gradients. Baruch I. Read More

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Membrane Env liposomes facilitate immunization with multivalent full-length HIV spikes.

J Virol 2021 Apr 21. Epub 2021 Apr 21.

Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA, USA.

A major goal of HIV vaccine design is to elicit broadly neutralizing antibodies (bnAbs). Such bnAbs target HIV's trimeric, membrane embedded envelope glycoprotein spikes, (m)Env. Soluble (s)Env trimers have been used as vaccines, but engineering sEnvs for stability, multivalency and desired antigenicity is problematic, and deletes key neutralizing epitopes on glycoprotein (gp)41 while creating neoepitopes that elicit unwanted antibodies. Read More

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Nanoliposomes for encapsulation and calcium delivery of egg white peptide-calcium complex.

J Food Sci 2021 Apr;86(4):1418-1431

National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian, China.

Nanoliposomes and crude liposomes loaded with egg white peptide-calcium complex (EWP-Ca) were fabricated by thin-film dispersion with or without dynamic high-pressure microfluidization. Their physiochemical properties, in vitro stability, and calcium release profiles were investigated in this study. Results showed that the EWP-Ca-loaded nanoliposomes exhibited spherical structures with a lower particle size and polydispersity index as well as a higher thermal stability as compared to the corresponding crude liposomes. Read More

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New insights on human Hsp70-escort protein 1: Chaperone activity, interaction with liposomes, cellular localizations and HSPA's self-assemblies remodeling.

Int J Biol Macromol 2021 Apr 16;182:772-784. Epub 2021 Apr 16.

Sao Carlos Institute of Chemistry, University of Sao Paulo, Sao Carlos, SP, Brazil. Electronic address:

The 70 kDa heat shock proteins (Hsp70) are prone to self-assembly under thermal stress conditions, forming supramolecular assemblies (SMA), what may have detrimental consequences for cellular viability. In mitochondria, the cochaperone Hsp70-escort protein 1 (Hep1) maintains mitochondrial Hsp70 (mtHsp70) in a soluble and functional state, contributing to preserving proteostasis. Here we investigated the interaction between human Hep1 (hHep1) and HSPA9 (human mtHsp70) or HSPA1A (Hsp70-1A) in monomeric and thermic SMA states to unveil further information about the involved mechanisms. Read More

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Amphipol-facilitated Elucidation of the Functional Tetrameric Complex of Full-length Cytochrome P450 CYP2B4 and NADPH-Cytochrome-P450 Oxidoreductase.

J Biol Chem 2021 Apr 8:100645. Epub 2021 Apr 8.

Department of Pharmacology, The University of Michigan Medical School, Ann Arbor, MI, 48109, USA. Electronic address:

Interactions of membrane-bound mammalian cytochromes P450 (CYPs) with NADPH-cytochrome P450 oxidoreductase (POR), which are required for metabolism of xenobiotics, are facilitated by membrane lipids. A variety of membrane mimetics, such as phospholipid liposomes and nanodiscs, have been used to simulate the membrane to form catalytically active CYP:POR complexes. However, the exact mechanism(s) of these interactions are unclear, due to the absence of structural information of full-length mammalian CYP:POR complexes in membranes. Read More

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Membrane fusion mediated by peptidic SNARE protein analogues: Evaluation of FRET-based bulk leaflet mixing assays.

J Pept Sci 2021 Apr 6:e3327. Epub 2021 Apr 6.

Institute of Organic and Biomolecular Chemistry, Georg-August-University of Göttingen, Göttingen, Germany.

Peptide-mediated membrane fusion is frequently studied with in vitro bulk leaflet mixing assays based on Förster resonance energy transfer (FRET). In these, customized liposomes with fusogenic peptides are equipped with lipids which are labeled with fluorophores that form a FRET pair. Since FRET is dependent on distance and membrane fusion comes along with lipid mixing, the assays allow for conclusions on the membrane fusion process. Read More

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Paclitaxel loading in cationic liposome vectors is enhanced by replacement of oleoyl with linoleoyl tails with distinct lipid shapes.

Sci Rep 2021 Mar 31;11(1):7311. Epub 2021 Mar 31.

Materials, Physics, and Molecular, Cellular, and Developmental Biology Department, University of California, Santa Barbara, CA, 93106, USA.

Lipid carriers of hydrophobic paclitaxel (PTX) are used in clinical trials for cancer chemotherapy. Improving their loading capacity requires enhanced PTX solubilization. We compared the time-dependence of PTX membrane solubility as a function of PTX content in cationic liposomes (CLs) with lipid tails containing one (oleoyl; DOPC/DOTAP) or two (linoleoyl; DLinPC/newly synthesized DLinTAP) cis double bonds by using microscopy to generate kinetic phase diagrams. Read More

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Preparation and characterization of liposomes double loaded with amphotericin B and amphotericin B/hydroxypropyl-beta-cyclodextrin inclusion complex.

Pharm Nanotechnol 2021 Mar 10. Epub 2021 Mar 10.

Department of Pharmaceutics, 13-15 Le Thanh Tong str., Hoankiem District, Hanoi. Vietnam.

Background: Amphotericin B (AMB) is water-insoluble polyene, which has a broad spectrum of antifungal activity. The hydrophobic drug only exits in the phospholipid bilayer, leading to a low-drug liposomal loading capacity.

Objectives: This study is designed to prepare water-soluble inclusion complex (IC) between AMB and cyclodextrin (CD) to formulate liposomal vesicles, double-loaded with drug molecules in the phospholipid bilayer and AMB/CD IC in the aqueous core. Read More

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Carboxylated polyamidoamine dendron-bearing lipid-based assemblies for precise control of intracellular fate of cargo and induction of antigen-specific immune responses.

Biomater Sci 2021 Apr 8;9(8):3076-3089. Epub 2021 Mar 8.

Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 5998531, Japan.

For the establishment of advanced medicines such as cancer immunotherapy, high performance carriers that precisely deliver biologically active molecules must be developed to target organelles of the cells and to release their contents there. From the viewpoint of antigen delivery, endosomes are important target organelles because they contain immune-response-related receptors and proteins of various types. To obtain carriers for precision endosome delivery, a novel type of polyamidoamine dendron-based lipid having pH-sensitive terminal groups was synthesized for this study. Read More

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Multicompartmental Lipopolyplex as Vehicle for Antigens and Genes Delivery in Vaccine Formulations.

Pharmaceutics 2021 Feb 19;13(2). Epub 2021 Feb 19.

Pharmacology Department, Faculty of Medicine, Universidad de Valencia, 46010 Valencia, Spain.

Vector design and its characterization is an area of great interest in current vaccine research. In this article, we have formulated and characterized a multicompartmental lipopolyplex, which associates multiple liposomes and polyplexes in the same complex. These particles allow the simultaneous delivery of lipid or water-soluble antigens associated with genes to the same cell, in much higher amounts than conventional lipopolyplexes. Read More

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February 2021

Stimulus-sensitive liposomal delivery system based on new 3,7-diazabicyclo[3.3.1]nonane derivatives.

Bioorg Med Chem Lett 2021 May 2;39:127871. Epub 2021 Mar 2.

Department of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Leninskie Gory, 1-3, Russia; Institute of Physiologically Active Compounds, Russian Academy of Sciences, 142432 Chernogolovka, Severny pr., 1, Russia. Electronic address:

3,7-Diazabicyclo[3.3.1]nonane scaffold can serve as a basis for the design of molecular switches stimulating the fast release of water soluble compounds under the influence of external factors from the liposomal containers having those switches incorporated into the lipid bilayer. Read More

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Nanocrystal-loaded liposome for targeted delivery of poorly water-soluble antitumor drugs with high drug loading and stability towards efficient cancer therapy.

Int J Pharm 2021 Apr 27;599:120418. Epub 2021 Feb 27.

Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health and Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, PR China; Hefei Cancer Hospital, Chinese Academy of Sciences, Hefei 230031, PR China; Precision Medicine Research Laboratory of Anhui Province, Hefei 230088, PR China; Institutes of Physical Science and Information Technology, Anhui University, Hefei 230601, PR China. Electronic address:

Nanocrystals (NCs) enable the delivery of poorly water-soluble drugs with improved dissolution and bioavailability. However, their uncontrolled release and instability make targeted delivery challenging. Herein, a nano-in-nano delivery system composed of a drug nanocrystal core and liposome shell (NC@Lipo) is presented, which merges the advantages of drug nanocrystals (high drug loading) and liposomes (easy surface functionalization and high stability) for targeted delivery of hydrophobic drugs to tumors. Read More

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Combination Immunotherapy With LIGHT and Interleukin-2 Increases CD8 Central Memory T-Cells In Vivo.

J Surg Res 2021 Feb 22;263:44-52. Epub 2021 Feb 22.

Division of Surgical Oncology, Department of Surgery, University of Illinois at Chicago, Chicago, Illinois. Electronic address:

Background: The generation of long-term durable tumor immunity and prolonged disease-free survival depends on the ability to generate and support CD8+ central memory T-cells. Microsatellite-stable colon cancer is resistant to currently available immunotherapies; thus, development of novel mechanisms to increase both lymphocyte infiltration and central memory formation are needed to improve outcomes in these patients. We have previously demonstrated that both interleukin-2 (IL-2) and LIGHT (TNFSF14) independently enhance antitumor immune responses and hypothesize that combination immunotherapy may increase the CD8+ central memory T-cell response. Read More

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February 2021

Comparison of cubosomes and liposomes for the encapsulation and delivery of curcumin.

Soft Matter 2021 Mar 24;17(12):3306-3313. Epub 2021 Feb 24.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, P. R. China.

Inverse bicontinuous cubic phase nanoparticles (cubosomes) have attracted significant attention in recent years, owing to their potential use as delivery vehicles for chemically fragile or poorly soluble drugs and nutraceuticals. Herein we have investigated the use of lipid nanoparticles as a delivery vehicle for curcumin, a compound with demonstrated anti-cancer properties. Curcumin is encapsulated within cubosomes comprised of several different lipid formulations, as well as phospholipid-based liposomes. Read More

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The Immunogenicity of Capsid-Like Particle Vaccines in Combination with Different Adjuvants Using Different Routes of Administration.

Vaccines (Basel) 2021 Feb 6;9(2). Epub 2021 Feb 6.

Centre for Medical Parasitology, Department of Immunology and Microbiology, University of Copenhagen, 1165 København, Denmark.

Capsid-like particle (CLP) displays can be used to enhance the immunogenicity of vaccine antigens, but a better understanding of how CLP vaccines are best formulated and delivered is needed. This study compared the humoral immune responses in mice elicited against two different vaccine antigens (a bacterial protein and a viral peptide) delivered on an AP205 CLP platform using six different adjuvant formulations. In comparison to antibody responses obtained after immunization with the unadjuvanted CLP vaccine, three of the adjuvant systems (neutral liposomes/monophosphoryl lipid A/quillaja saponaria 21, squalene-in-water emulsion, and monophosphoryl lipid A) caused significantly increased antibody levels, whereas formulation with the three other adjuvants (aluminum hydroxide, cationic liposomes, and cationic microparticles) resulted in similar or even decreased antibody responses. Read More

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February 2021

A facile and universal method to achieve liposomal remote loading of non-ionizable drugs with outstanding safety profiles and therapeutic effect.

Acta Pharm Sin B 2021 Jan 13;11(1):258-270. Epub 2020 Aug 13.

Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, China.

Liposomes have made remarkable achievements as drug delivery vehicles in the clinic. Liposomal products mostly benefited from remote drug loading techniques that succeeded in amphipathic and/or ionizable drugs, but seemed impracticable for nonionizable and poorly water-soluble therapeutic agents, thereby impeding extensive promising drugs to hitchhike liposomal vehicles for disease therapy. In this study, a series of weak acid drug derivatives were designed by a simplistic one step synthesis, which could be remotely loaded into liposomes by pH gradient method. Read More

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January 2021

A Novel Drug Delivery System: the Encapsulation of Naringenin in Metal-Organic Frameworks into Liposomes.

AAPS PharmSciTech 2021 Feb 1;22(2):61. Epub 2021 Feb 1.

Chemistry College, Sichuan University, Chengdu, 610064, China.

Poorly water-soluble naringenin (NAR) was selected as a model drug and loaded into the porous MOFs for the construction of NAR@ZIF-8 inclusion complex. By film dispersion method, NAR@ZIF-8 was further encapsulated into liposomes to fabricate a novel drug delivery system. Liposomes and a novel drug delivery system were established. Read More

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February 2021

Emerging Nanomedicines for the Treatment of Atopic Dermatitis.

AAPS PharmSciTech 2021 Jan 24;22(2):55. Epub 2021 Jan 24.

School of Pharmacy, The University of Queensland, Woolloongabba, 4102, Australia.

Globally, the prevalence of Atopic dermatitis (AD) is significantly increasing and affecting around 20% of population including children. Complex interactions amongst abnormality in epidermal barrier function, environment, infectious agents and immunological defects are considered as key factors in the pathogenesis of AD. Although the role of oxidative stress has been studied in some skin diseases, investigation of the same in AD is intermittent. Read More

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January 2021

pH - Responsive colloidal carriers assembled from β-lactoglobulin and Epsilon poly-L-lysine for oral drug delivery.

J Colloid Interface Sci 2021 May 21;589:45-55. Epub 2020 Dec 21.

School of Pharmacy, The University of Queensland, Brisbane, QLD 4102, Australia; Mater Research Institute - The University of Queensland, Translational Research Institute, 37 Kent St, Woolloongabba, QLD 4102, Australia. Electronic address:

Site specific oral delivery of many biopharmaceutical classification system (BCS) class II and IV drugs is challenging due to their poor solubility, low permeability and degradation in the gastrointestinal tract. Whilst colloidal carriers have been used to improve the bioavailability of such drugs, most nanocarriers based drug delivery systems suffer from multiple disadvantages, including low encapsulation efficiency (liposomes, polymeric nanoparticles), complex synthesis methods (silica, silicon-based materials) and poorly understood biodegradability (inorganic nanoparticles). Herein, a novel pH responsive nanocolloids were self-assembled using natural compounds such as bovine β-lactoglobulin (BLG) and succinylated β-lactoglobulin (succ. Read More

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Liposomal drug delivery of Corchorus olitorius leaf extract containing phytol using design of experiment (DoE): In-vitro anticancer and in-vivo anti-inflammatory studies.

Colloids Surf B Biointerfaces 2021 Mar 24;199:111543. Epub 2020 Dec 24.

Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, 11451, KSA. Electronic address:

Phytol, a pharmacologically active compound present in Corchorus olitorius leaf exhibit a range of activity including anti-inflammatory, antioxidant, anticancer, hepatoprotective etc. However, phytol is poorly soluble and absorbed through the intestine wall, therefore the aim of this study is to develop liposomal drug delivery of Corchorus olitorius leaf extract with an average particle size below 150 nm and drug loading efficiency of ≥ 85 %. The impact of different process parameters and material attributes were studied on the average particle size and polydispersity of liposomal batches using design of experiment (DoE). Read More

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Spatially regulated activation of membrane fusogenic peptides with chaperone-like ionic copolymers.

J Control Release 2021 Feb 25;330:463-469. Epub 2020 Dec 25.

School of Life Science and Technology, Tokyo Institute of Technology, 4259 B-57, Nagatsuta, Midori, Yokohama 226-8501, Japan. Electronic address:

Controlled or targeted membrane lysis induced by cascades of assembly and activation of biomolecules on membrane surfaces is important in programmed cell death and host defense systems. In a previous study, we reported that an ionic graft copolymer with a polycation backbone and water-soluble graft chains, poly(allylamine)-graft-dextran (PAA-g-Dex) chaperoned folding and assembly of E5, a membrane-destructive peptide derived from influenza hemagglutinin, to its increase membrane-disruptive activity. In this study, we modified the copolymer with long acyl chains, which resulted in delivery of the copolymer to membrane surfaces of liposomes and living cells. Read More

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February 2021

Effects of Linkers on the Development of Liposomal Formulation of Cholesterol Conjugated Cobalt Bis(dicarbollides).

J Pharm Sci 2021 03 17;110(3):1365-1373. Epub 2020 Dec 17.

Department of Chemistry & Biochemistry, Northern Illinois University, DeKalb, IL 60115, USA.

Boron neutron capture therapy (BNCT) remains an important treatment arm for cancer patients with locally invasive malignant tumors. This therapy needs a significant amount of boron to deposit in cancer tissues selectively, sparing other healthy organs. Most of the liposomes contain water-soluble polyhedral boron salts stay in the core of the liposomes and have low encapsulation efficiency. Read More

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Influence of drug loading on the physical stability of phospholipid-stabilised colloidal lipid emulsions.

Int J Pharm X 2020 Dec 20;2:100060. Epub 2020 Nov 20.

Technische Universität Braunschweig, Institut für Pharmazeutische Technologie und Biopharmazie, Mendelssohnstraße 1, 38106 Braunschweig, Germany.

When poorly water-soluble drugs are formulated in colloidal lipid emulsions, adequate stability of the emulsion must be ensured. The aim of this work was to investigate different aspects related to drug loading in order to gain a better understanding on how drugs affect the stability of phospholipid-stabilised emulsions. To obtain information on emulsion stability, a rapid and reproduceable shaking test was developed. Read More

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December 2020

Assessing the pharmacokinetics and toxicology of polymeric micelle conjugated therapeutics.

Expert Opin Drug Metab Toxicol 2021 Mar 21;17(3):323-332. Epub 2020 Dec 21.

Department of Pharmacy, School of Chemical Sciences and Pharmacy, Central University of Rajasthan, Ajmer, Rajasthan, India.

: Analogous to nanocarriers such as nanoparticles, liposomes, nano lipoidal carriers, niosomes, and ethosomes, polymeric micelles have gained significance in the field of drug delivery. They have attracted scientists worldwide by their nanometric size, wide range of polymers available for building block synthesis, stability and potential to enhance the targeting and safety of drugs. Incorporation of drugs within the interior of polymeric micelles alters the drug pharmacokinetics, which generally results in increased efficiency. Read More

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