59 results match your criteria sk-mel28 cells

Cell-based electrochemical cytosensor for rapid and sensitive evaluation of the anticancer effects of saponin on human malignant melanoma cells.

Bioelectrochemistry 2021 Aug 31;140:107813. Epub 2021 Mar 31.

Department of Bionano Engineering, Center for Bionano Intelligence Education and Research, Hanyang University, Ansan 426-791, South Korea. Electronic address:

Discovering new anticancer agents and analyzing their activities is a vital part of drug development, but it requires a huge amount of time and resources, leading to the increasing demands for more-effective techniques. Herein, a novel and simple cell-based electrochemical biosensor, referred to as a cytosensor, was proposed to investigate the electrochemical behavior of human skin malignant melanoma (SK-MEL28) cells and the anticancer effect of saponin on cell viability. To enhance both electrocatalytic properties and biocompatibility, gold nanoparticles were electrochemically deposited onto a conductive substrate, and poly-L-lysine was further added to the electrode surface. Read More

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Innovative hydrogel containing polymeric nanocapsules loaded with phloretin: Enhanced skin penetration and adhesion.

Mater Sci Eng C Mater Biol Appl 2021 Jan 28;120:111681. Epub 2020 Oct 28.

Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil. Electronic address:

Dermatological applications of phloretin are restricted by its poor aqueous solubility. Nanotechnology has been proposed as strategy to increase the apparent drug solubility in aqueous media. This study aimed to develop, characterize, and evaluate the antitumoral effects and safety of polymeric nanocapsules containing phloretin (NCPhl). Read More

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January 2021

Integrin αvβ3-Akt signalling plays a role in radioresistance of melanoma.

Exp Dermatol 2020 06 4;29(6):562-569. Epub 2020 May 4.

Division of Radiation Biomedical Research, Korea Institute of Radiological and Medical Sciences, Seoul, Korea.

Melanoma is a deadly type of skin cancer that is particularly difficult to treat owing to its resistance to radiation therapy. Here, we attempted to determine the key proteins responsible for melanoma radioresistance, with the aim of improving disease response to radiation therapy. Two melanoma cell lines, SK-Mel5 and SK-Mel28, with different radiosensitivities were analysed via RNA-Seq (Quant-Seq) and target proteins with higher abundance in the more radioresistant cell line, SK-Mel28, identified. Read More

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Profiling of -acetylated Gangliosides Expressed in Neuroectoderm Derived Cells.

Int J Mol Sci 2020 Jan 6;21(1). Epub 2020 Jan 6.

Univ. Lille, CNRS, UMR 8576-UGSF-Unité de Glycobiologie Structurale et Fonctionnelle, F-59000 Lille, France.

The expression and biological functions of oncofetal markers GD2 and GD3 were extensively studied in neuroectoderm-derived cancers in order to characterize their potential as therapeutic targets. Using immunological approaches, we previously identified GD3, GD2, and AcGD2 expression in breast cancer (BC) cell lines. However, antibodies specific for -acetylated gangliosides are not exempt of limitations, as they only provide information on the expression of a limited set of -acetylated ganglioside species. Read More

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January 2020

Enhancing radiosensitivity of melanoma cells through very high dose rate pulses released by a plasma focus device.

PLoS One 2018 29;13(6):e0199312. Epub 2018 Jun 29.

University of Bologna, Department of Physics and Astronomy, Bologna, Italy.

Radiation therapy is a useful and standard tumor treatment strategy. Despite recent advances in delivery of ionizing radiation, survival rates for some cancer patients are still low because of recurrence and radioresistance. This is why many novel approaches have been explored to improve radiotherapy outcome. Read More

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Targeting the oncogene impairs proliferation and invasiveness of melanoma cells sensitive or with acquired resistance to the BRAF inhibitor dabrafenib.

Oncotarget 2017 Dec 9;8(69):113472-113493. Epub 2017 Dec 9.

Laboratory of Molecular Oncology, Istituto Dermopatico dell'Immacolata-IRCCS, Rome, Italy.

The () is implicated in tumor growth, metastasis and drug resistance. Here, we investigated the involvement of in melanoma cell proliferation, invasiveness and response to the BRAF inhibitor (BRAFi) dabrafenib. We also preliminary assessed the potential value of circulating PTTG1 protein to monitor melanoma patient response to BRAFi or to dabrafenib plus trametinib. Read More

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December 2017

Targeting Insulin-Like Growth Factor-I and Extracellular Matrix Interactions in Melanoma Progression.

Sci Rep 2018 01 12;8(1):583. Epub 2018 Jan 12.

Institute of Health and Biomedical Innovation, School of Biomedical Science, Queensland University of Technology, Brisbane, QLD, Australia.

Insulin-like growth factor (IGF)-I binds to the ECM protein vitronectin (VN) through IGF binding proteins (IGFBPs) to enhance proliferation and migration of skin keratinocytes and fibroblasts. Although evidence exists for the role of individual components of the complex (IGF-I, IGFBP-3 and VN), the cellular functions stimulated by these proteins together as a complex remains un-investigated in melanoma cells. We report here that the IGF-I:IGFBP-3:VN trimeric complex stimulates a dose-dependent increase in the proliferation and migration of WM35 and Sk-MEL28 melanoma cells. Read More

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January 2018

Isolation and Structural Characterization of Echinocystic Acid Triterpenoid Saponins from the Australian Medicinal and Food Plant Acacia ligulata.

J Nat Prod 2017 10 4;80(10):2692-2698. Epub 2017 Oct 4.

Sansom Institute for Health Research, School of Pharmacy and Medical Sciences, University of South Australia , Adelaide, South Australia 5000, Australia.

The Australian plant Acacia ligulata has a number of traditional food and medicinal uses by Australian Aboriginal people, although no bioactive compounds have previously been isolated from this species. Bioassay-guided fractionation of an ethanolic extract of the mature pods of A. ligulata led to the isolation of the two new echinocystic acid triterpenoid saponins, ligulatasides A (1) and B (2), which differ in the fine structure of their glycan substituents. Read More

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October 2017

Bioselection of coxsackievirus B6 strain variants with altered tropism to human cancer cell lines.

Arch Virol 2017 Nov 1;162(11):3355-3362. Epub 2017 Aug 1.

Novosibirsk State University, Novosibirsk, Russia.

Cancer cells develop increased sensitivity to members of many virus families and, in particular, can be efficiently infected and lysed by many low-pathogenic human enteroviruses. However, because of their great genetic heterogeneity, cancer cells display different levels of sensitivity to particular enterovirus strains, which may substantially limit the chances of a positive clinical response. We show that a non-pathogenic strain of coxsackievirus B6 (LEV15) can efficiently replicate to high titers in the malignant human cell lines C33A, DU145, AsPC-1 and SK-Mel28, although it displays much lower replication efficiency in A431 and A549 cells and very limited replication ability in RD and MCF7 cells, as well as in the normal lung fibroblast cell line MRC-5 and the immortalized mammary epithelial cell line MCF10A. Read More

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November 2017

Antimicrobial, anticancer, and antioxidant compounds from Premna resinosa growing in Saudi Arabia.

Pharm Biol 2017 Dec;55(1):1759-1766

b Department of Pharmacognosy, College of Pharmacy , King Saud University , Riyadh , Saudi Arabia.

Context: Premna resinosa (Hochst.) Schauer (Lamiaceae) is used in many places to treat bronchitis, respiratory illness and convulsions of the rib cage.

Objective: This study evaluates the anticancer, antimicrobial and antioxidant activities of P. Read More

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December 2017

Cryptolepine inhibits melanoma cell growth through coordinated changes in mitochondrial biogenesis, dynamics and metabolic tumor suppressor AMPKα1/2-LKB1.

Sci Rep 2017 05 4;7(1):1498. Epub 2017 May 4.

Department of Dermatology, University of Alabama at Birmingham, Birmingham, AL, USA.

Dysregulated mitochondrial dynamics and biogenesis have been associated with various pathological conditions including cancers. Here, we assessed the therapeutic effect of cryptolepine, a pharmacologically active alkaloid derived from the roots of Cryptolepis sanguinolenta, on melanoma cell growth. Treatment of human melanoma cell lines (A375, Hs294t, SK-Mel28 and SK-Mel119) with cryptolepine (1. Read More

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Biological activity and LC-MS/MS profiling of extracts from the Australian medicinal plant Acacia ligulata (Fabaceae).

Nat Prod Res 2018 Mar 21;32(5):576-581. Epub 2017 Apr 21.

a School of Pharmacy and Medical Sciences, Quality Use of Medicines and Pharmacy Research Centre , Sansom Institute for Health Research, University of South Australia , Adelaide , Australia.

Acacia ligulata A.Cunn. ex Benth. Read More

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Efficient ferrocifen anticancer drug and Bcl-2 gene therapy using lipid nanocapsules on human melanoma xenograft in mouse.

Pharmacol Res 2017 Dec 31;126:54-65. Epub 2017 Jan 31.

MINT, UNIV Angers, INSERM, CNRS, Université Bretagne Loire, IBS-CHU, 4 rue Larrey, F-49933 Angers, France. Electronic address:

Metastatic melanoma has been described as a highly aggressive cancer with low sensibility to chemotherapeutic agents. New types of drug, such as metal-based drugs (ferrocifens) have emerged and could represent an alternative for melanoma treatment since they show interesting anticancer potential. Furthermore, molecular analysis has evidenced the role of apoptosis in the low sensibility of melanomas and especially of the key regulator, Bcl-2. Read More

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December 2017

Suppression of B-Raf melanoma cell survival by targeting mitochondria using triphenyl-phosphonium-conjugated nitroxide or ubiquinone.

Cancer Biol Ther 2017 02 27;18(2):106-114. Epub 2016 Oct 27.

a Department of Biochemistry , Medical College of Wisconsin , Milwaukee , WI , USA.

Most BRAF-mutated melanomas initially responsive to the FDA-approved inhibitors preferentially targeting B-Raf mutated in Val600 residue eventually relapse, requiring additional therapeutic modalities. Recent studies report the significance of metabolic reprograming in mitochondria for maintenance of BRAF-mutated melanomas and for development of their drug resistance to B-Raf inhibitors, providing a rationale for targeting mitochondria as a potential therapeutic strategy for melanoma. We therefore determined whether mitochondria-targeted metabolism-interfering agents can effectively suppress human B-Raf melanoma cell lines and their dabrafenib/PLX4032-resistant progenies using mitochondria-targeted carboxy-proxyl (Mito-CP) and ubiquinone (Mito-Q). Read More

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February 2017

Labeling Human Melanoma Cells With SPIO: In Vitro Observations.

Mol Imaging 2016 29;15. Epub 2016 Jan 29.

Department of Diagnostic and Interventional Radiology, Eberhard-Karls-University Tübingen, Hoppe-Seyler-Str. 3, 72076 Tübingen, Germany.

Objectives: To use the superparamagnetic iron oxide (SPIO) contrast agent Resovist (±transfection agent) to label human melanoma cells and determine its effects on cellular viability, microstructure, iron quantity, and magnetic resonance imaging (MRI) detectability.

Materials And Methods: Human SK-Mel28 melanoma cells were incubated with Resovist (±liposomal transfection agent DOSPER). The cellular iron content was measured, and labeled cells were examined at 1. Read More

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December 2016

A second-generation ferrocene-iminosugar hybrid with improved fucosidase binding properties.

Bioorg Med Chem Lett 2016 Mar 9;26(6):1546-1549. Epub 2016 Feb 9.

Université de Reims Champagne-Ardenne, Institut de Chimie Moléculaire de Reims (ICMR), CNRS UMR 7312, UFR Sciences Exactes et Naturelles, BP 1039, 51687 Reims Cedex 2, France. Electronic address:

The synthesis and the biological evaluation of a new ferrocenyl-iminosugar conjugate designed for fucosidase inhibitory and anticancer activity is described. The compound showed strong affinity for fucosidase from bovine kidney (Ki=23 nM) and from Bacteroides thetaiotaomicron (Ki=150 nM), displaying a 10-fold tighter binding affinity for these enzymes than the previous analogs. The interaction pattern that improves binding has been evaluated through structural analysis of the inhibitor-enzyme complex. Read More

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Inhibition of NADPH oxidase 1 activity and blocking the binding of cytosolic and membrane-bound proteins by honokiol inhibit migratory potential of melanoma cells.

Oncotarget 2016 Feb;7(7):7899-912

Department of Dermatology, University of Alabama at Birmingham, Birmingham, AL, USA.

Overexpression of NADPH oxidase 1 (Nox1) in melanoma cells is often associated with increased migration/metastasis rate. To develop effective treatment options, we have examined the effect of honokiol, a phytochemical from Magnolia plant, on the migratory potential of human melanoma cell lines (A375, Hs294t, SK-Mel119 and SK-Mel28) and assessed whether Nox1 is the target. Using an in vitro cell migration assay, we observed that treatment of different melanoma cell lines with honokiol for 24 h resulted in a dose-dependent inhibition of cell migration that was associated with reduction in Nox1 expression and reduced levels of oxidative stress. Read More

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February 2016

Suppressor of cytokine signalling-1 induces significant preclinical antitumor effect in malignant melanoma cells.

Exp Dermatol 2015 Nov 3;24(11):864-71. Epub 2015 Sep 3.

Laboratory of Immune Signal, National Institute of Biomedical Innovation, Ibaraki, Japan.

Malignant melanoma is the most aggressive form of skin cancer, responsible for the majority of skin cancer-related deaths. Metastatic melanoma is resistant to surgery, radiation or chemotherapy, and an effective therapy has not yet been established. Our study investigated the therapeutic potential of the suppressor of cytokine signalling-1 (SOCS-1), an endogenous inhibitor of the intracellular cytokine signalling pathway, for treating melanoma. Read More

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November 2015

Sensitivity of neoplastic cells to senescence unveiled under standard cell culture conditions.

Anticancer Res 2015 May;35(5):2759-68

Department of Tumor Biology, Medical University of Lodz, Lodz, Poland.

Background: Cancer cells are typically defined as infinitely proliferating, whereas normal cells (except stem cells) are considered as being programmed to become senescent. Our data show that this characterization is misleading.

Materials And Methods: Multiplex Ligation-dependent Probe Amplification, TP53 sequencing, real-time polymerase chain reaction (PCR) for MUC1 and SCGB2A2 and immunocytochemistry, together with senescence detection assay and real-time microscopic observations were used to analyze primary neoplastic cells isolated from prostate, breast and colorectal tumors, as well as stable cancer cell lines (MCF7, MDA-MB-468, SW962, SK-MEL28, NCI-H1975 and NCI-H469). Read More

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Polyphenols from green tea inhibit the growth of melanoma cells through inhibition of class I histone deacetylases and induction of DNA damage.

Genes Cancer 2015 Jan;6(1-2):49-61

Department of Dermatology, University of Alabama at Birmingham, Birmingham, AL, USA ; Environmental Health Sciences, University of Alabama at Birmingham, Birmingham, AL, USA ; Nutrition Obesity Research Center, School of Public Health, University of Alabama at Birmingham, Birmingham, AL, USA ; Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL, USA ; Birmingham Veterans Affairs Medical Center, Birmingham, AL, USA.

Melanoma is the leading cause of skin cancer-related deaths. We have examined the effect of green tea polyphenols (GTPs), a natural mixture of epicatechin monomers, on melanoma cancer cell growth and the molecular mechanism underlying these effects using different human melanoma cell lines as an in vitro model. Treatment of melanoma cell lines (A375, Hs294t, SK-Mel28 and SK-Mel119) with GTPs significantly inhibited the cell viability as well as colony formation ability of melanoma cells in a dose-dependent manner. Read More

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January 2015

Sp1 regulates Raf/MEK/ERK-induced p21(CIP1) transcription in TP53-mutated cancer cells.

Cell Signal 2015 Mar 13;27(3):479-86. Epub 2015 Jan 13.

Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI 53226, USA. Electronic address:

We previously reported that the upregulation of mortalin, an Hsp70 family chaperone, is important for B-Raf(V600E) tumor cells to bypass p21(CIP1) expression, which is activated as a tumor-suppressive mechanism in response to aberrant MEK/ERK activation (Wu et al., 2013). Interestingly, mortalin depletion induced p21(CIP1) transcription not only in wild-type TP53 but also in TP53-mutated B-Raf(V600E) cancer cells, suggesting the presence of an additional mechanism for p21(CIP1) regulation. Read More

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Down-regulation of miRNA-106b inhibits growth of melanoma cells by promoting G1-phase cell cycle arrest and reactivation of p21/WAF1/Cip1 protein.

Oncotarget 2014 Nov;5(21):10636-49

Department of Dermatology, University of Alabama at Birmingham, Birmingham, AL, USA. Environmental Health Sciences, University of Alabama at Birmingham, Birmingham, AL, USA. Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL, USA. Birmingham Veterans Affairs Medical Center, Birmingham, AL, USA.

MiR-106b is overexpressed in various types of cancers and is associated with the regulation of the carcinogenic processes. Using RT-PCR, we have identified overexpression of miRNA-106b in various melanoma cell lines (A375, Hs294t, SK-Mel28, SK-Mel 119, Mel 1241, Mel 1011 and Mel 928) as compared to its expression in normal human epidermal melanocytes (NHEM). The overexpression of miR-106b in melanoma cells (A375, Hs294t) was associated with greater cell proliferation capacity than NHEM. Read More

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November 2014

Efficient in vitro gene therapy with PEG siRNA lipid nanocapsules for passive targeting strategy in melanoma.

Biotechnol J 2014 Nov 18;9(11):1389-401. Epub 2014 Oct 18.

PRES LUNAM, Universit é d'Angers, Angers, France; INSERM, Micro et Nanomédecines Biomimétiques, Angers, France.

Small interfering RNA (siRNA)-mediated gene therapy is a promising strategy to temporarily inhibit the expression of proteins implicated in carcinogenesis or chemotherapy resistance. Although intra-tumoral administration can be envisaged, studies currently focus on formulating nanomedicines for intravenous injection to target tumor sites as well as metastases. The development of synthetic nanoparticles and liposomes has advanced greatly during the last decade. Read More

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November 2014

Expression and subcellular localization of RAGE in melanoma cells.

Biochem Cell Biol 2014 Apr 31;92(2):127-36. Epub 2014 Mar 31.

Institute of Biochemistry of the Romanian Academy, Splaiul Independentei 296, Bucharest 060031, Romania.

The receptor for advanced glycation end products (RAGE) is involved in multiple stages of tumor development and malignization. To gain further knowledge on the RAGE role in tumor progression, we investigated the receptor expression profile and its subcellular localization in melanoma cells at different stages of malignancy. We found that RAGE clustered at membrane ruffles and leading edges, and at sites of cell-to-cell contact in primary melanoma cells (e. Read More

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GNG2 inhibits invasion of human malignant melanoma cells with decreased FAK activity.

Am J Cancer Res 2014 1;4(2):182-8. Epub 2014 Mar 1.

Department of Occupational and Environmental Health, Nagoya University Graduate School of Medicine 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan ; Units of Environmental Health Sciences, Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University Kasugai, Aichi 487-8501, Japan.

It is well known that heterotrimeric G protein is composed of a Gα-subunit and a Gβγ-dimer and promotes cancer characteristics. Our recent study showed reduced G protein γ2 subunit (Gng2/GNG2) expression levels in malignant melanoma cells compared with those in benign melanocytic cells in both mice and humans. Our recent study also showed that reduced GNG2 alone augmented proliferation of malignant melanoma cells. Read More

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Phenotyping of human melanoma cells reveals a unique composition of receptor targets and a subpopulation co-expressing ErbB4, EPO-R and NGF-R.

PLoS One 2014 29;9(1):e84417. Epub 2014 Jan 29.

Ludwig Boltzmann Cluster Oncology, Medical University of Vienna, Vienna, Austria ; Division of Hematology & Hemostaseology, Department of Medicine I, Medical University of Vienna, Vienna, Austria.

Malignant melanoma is a life-threatening skin cancer increasingly diagnosed in the western world. In advanced disease the prognosis is grave. Growth and metastasis formation in melanomas are regulated by a network of cytokines, cytokine-receptors, and adhesion molecules. Read More

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September 2014

Fully-automated radiosynthesis and in vitro uptake investigation of [N-methyl-¹¹C]methylene blue.

Anticancer Res 2013 Oct;33(10):4267-70

University of Aberdeen, School of Medicine & Dentistry, John Mallard Scottish PET Centre, Foresterhill, Aberdeen AB25 2ZD, U.K.

Malignant melanoma is a type of skin cancer which can spread rapidly if not detected early and left untreated. Positron Emission Tomography (PET) is a powerful imaging technique for detecting cancer but with only a limited number of radiotracers available the development of novel PET probes for detection and prevention of cancer is imperative. In the present study we present the fully-automated radiosynthesis of [N-methyl-(11)C]methylene blue and an in vitro uptake study in metastasic melanoma cell lines. Read More

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October 2013

Gene electrotransfer of siRNAs against CD146 inhibits migration and invasion of human malignant melanoma cells SK-MEL28.

Cancer Gene Ther 2013 Mar 1;20(3):208-10. Epub 2013 Feb 1.

Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska cesta 2, Ljubljana, Slovenia.

Targeting molecules involved in tumor invasion may be useful in future strategies for melanoma treatment aiming to reduce the progression of the disease and prevention of metastatic spread. During melanoma progression to metastatic disease, a significant overexpression of melanoma cell adhesion molecule CD146 occurs. It has been correlated with tumor progression and metastatic potential. Read More

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Role of Ser129 phosphorylation of α-synuclein in melanoma cells.

J Cell Sci 2013 Jan 30;126(Pt 2):696-704. Epub 2012 Nov 30.

Department of Medicine, GRU Cancer Center, Georgia Regents University, Augusta, GA 30912, USA.

α-Synuclein, a protein central to Parkinson's disease, is frequently expressed in melanoma tissues, but not in non-melanocytic cutaneous carcinoma and normal skin. Thus, α-synuclein is not only related to Parkinson's disease, but also to melanoma. Recently, epidemiologists reported co-occurrence of melanoma and Parkinson's disease in patients, suggesting that these diseases could share common pathogenetic components and that α-synuclein might be one of these. Read More

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January 2013

Functional analysis of GNG2 in human malignant melanoma cells.

J Dermatol Sci 2012 Dec 18;68(3):172-8. Epub 2012 Sep 18.

Unit of the Environmental Health Sciences, Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, Japan.

Background: Previous studies have revealed that heterotrimeric G protein is composed of a Gα-subunit and a Gβγ-dimer and is correlated with c-Src and AKT activities. Our recent study showed reduced G protein γ2 subunit (Gng2/GNG2) expression levels in malignant melanoma cells compared with those in benign melanocytic cells in both mice and humans. At present, however, there is no evidence showing an effect of Gng2/GNG2 alone on cancer biology. Read More

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December 2012