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Rigor, reproducibility and transparency of randomized controlled trials in obstetrics and gynecology.

Am J Obstet Gynecol MFM 2021 Jul 26:100450. Epub 2021 Jul 26.

University of Utah Health, Department of Obstetrics and Gynecology, Salt Lake City, UT; Intermountain Healthcare, Department of Obstetrics and Gynecology, Murray, UT.

Background: Randomized controlled trials are considered the highest level of evidence but fewer than half are reproducible. Rigorous methodology improves trial quality but reproducibility may be limited by lack of transparency in reporting. The Consolidated Standard of Reporting Trials (CONSORT) guidelines define reporting standards, and pre-trial registration requires pre-defined methodology and outcomes. Read More

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Electrostatic Spray Drying for Monoclonal Antibody Formulation.

Int J Pharm 2021 Jul 26:120942. Epub 2021 Jul 26.

Department of Industrial and Physical Pharmacy, College of Pharmacy, Purdue University, 575 Stadium Mall Drive, West Lafayette, IN 47907, USA. Electronic address:

This study explored the feasibility of electrostatic spray drying for producing a monoclonal antibody (mAb) powder formulation at lower drying temperatures than conventional spray drying and its effect on protein stability. A mAb formulation was dried by either conventional spray drying or electrostatic spray drying with charge (ESD). The protein powders were then characterized using solid-state Fourier transform infrared spectroscopy (ssFTIR), differential scanning calorimetry (DSC), size exclusion chromatography (SEC), and solid-state hydrogen/deuterium exchange with mass spectrometry (ssHDX-MS). Read More

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Apolipoprotein A-I modulates HDL particle size in the absence of apolipoprotein A-II.

J Lipid Res 2021 Jul 26:100099. Epub 2021 Jul 26.

Center for Lipid and Arteriosclerosis Science, Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio 45237. Electronic address:

Human high-density lipoproteins (HDL) are a complex mixture of structurally-related nanoparticles that perform distinct physiological functions. We previously showed human HDL containing apolipoprotein A-I (APOA1) but not apolipoprotein A-II (APOA2), designated LpA-I, is composed primarily of two discretely sized populations. Here, we isolated these particles directly from human plasma by antibody affinity chromatography, separated them by high-resolution size exclusion chromatography and performed a deep molecular characterization of each species. Read More

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One step further: randomised single-centre trial comparing the direct and afterload techniques of embryo transfer.

Hum Reprod 2021 Jul 29. Epub 2021 Jul 29.

Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University, School of Medicine, New Haven, CT, USA.

Study Question: What are the differences in ease of use between two different embryo transfer (ET) techniques: the preload direct approach and the afterload approach.

Summary Answer: The afterload technique seems to reduce the rate of difficult ETs.

What Is Known Already: Numerous published trials now document that the ET procedure has an impact on pregnancy and delivery rates after IVF. Read More

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Molecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin.

J Extracell Vesicles 2021 Jul 22;10(9):e12128. Epub 2021 Jul 22.

Department of Clinical Immunology and Transfusion Medicine and Division of Immunology and Allergy, Department of Medicine Solna Karolinska University Hospital and Karolinska Institutet Stockholm Sweden.

Extracellular vesicles (EVs) are increasingly tested as therapeutic vehicles and biomarkers, but still EV subtypes are not fully characterised. To isolate EVs with few co-isolated entities, a combination of methods is needed. However, this is time-consuming and requires large sample volumes, often not feasible in most clinical studies or in studies where small sample volumes are available. Read More

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Radical hysterectomy in early cervical cancer in Europe: characteristics, outcomes and evaluation of ESGO quality indicators.

Int J Gynecol Cancer 2021 Jul 28. Epub 2021 Jul 28.

Division of Gynecologic Oncology, 1st Department of Obstetrics and Gynecology, Alexandra Hospital, National and Kapodistrian University of Athens, Athens, Greece.

Introduction: Comprehensive updated information on cervical cancer surgical treatment in Europe is scarce.

Objective: To evaluate baseline characteristics of women with early cervical cancer and to analyze the outcomes of the ESGO quality indicators after radical hysterectomy in the SUCCOR database.

Methods: The SUCCOR database consisted of 1272 patients who underwent radical hysterectomy for stage IB1 cervical cancer (FIGO 2009) between January 2013 and December 2014. Read More

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Evaluation of repeat distal transradial access in the anatomic snuffbox.

Diagn Interv Radiol 2021 Jul 28. Epub 2021 Jul 28.

Division of Vascular - Interventional Radiology, Department of Radiology, Duke University Medical Center, Durham, North Carolina, USA.

Purpose: There is increasing interest in the distal radial artery in the anatomic snuffbox as an alternative arterial access point, but the durability of the distal radial artery to support repetitive accesses over multiple procedures is not well established. The purpose of this study was therefore to evaluate success rates for repeated left-sided distal transradial access (ldTRA) in the anatomic snuffbox.

Methods: In this single institution retrospective study, all patients undergoing radioembolization treatments from January 1st, 2019 to May 1st, 2020 were prospectively evaluated for ldTRA. Read More

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Castor oil as booster for colon capsule endoscopy preparation reduction: A prospective pilot study and patient questionnaire.

World J Gastrointest Pharmacol Ther 2021 Jul;12(4):79-89

Department of Gastroenterology and Hepatology, Kindai University Faculty of Medicine, Osaka 589-8511, Japan.

Background: Preparation for colon capsule endoscopy (CCE) requires a large liquid laxative volume for capsule excretion, which compromises the procedure's tolerability.

Aim: To assess the safety and utility of castor oil-boosted bowel preparation.

Methods: This prospective cohort study including 20 patients (age range, 16-80 years; six men and 14 women) suspected of having colorectal disease was conducted at Kindai University Hospital from September 2017 to August 2019. Read More

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Tailored nanodisc immobilization for one-step purification and reconstitution of cytochrome P450: A tool for membrane proteins' hard cases.

J Sep Sci 2021 Jul 26. Epub 2021 Jul 26.

State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China.

A novel nanodisc-based immobilization method was developed for high-efficient purification and reconstitution of cytochrome P450 in one step. Using membrane scaffold protein containing a histidine tag, charged-nanodiscs were prepared in the form of self-assembly of lipid-protein nanoparticles. Their properties including the particle diameter and its distribution, and Zeta potential were controlled well by adjusting molar ratios of phospholipids to membrane scaffold protein. Read More

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Characterization of Dissolved Organic Matter and Its Derived Disinfection Byproduct Formation along the Yangtze River.

Environ Sci Technol 2021 Jul 23. Epub 2021 Jul 23.

State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Yangpu District, Shanghai 200092, China.

The Yangtze River basin covers one-fifth of China's land area and serves as a water source for one-third of China's population. During long-distance water transport from upstream to downstream, various sources of dissolved organic matter (DOM) lead to considerable variation in DOM properties, significantly impacting water treatability and disinfection byproduct (DBP) formation after chlorination. Using size-exclusion chromatography and fluorescence spectroscopy, the spatial variation in DOM characteristics was comprehensively investigated on a basin scale. Read More

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Extracorporeal photopheresis chargemasters show haphazard billing practices.

Transfusion 2021 Jul 23. Epub 2021 Jul 23.

Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Background: United States healthcare spending continues to outpace other developed nations although efforts are being made to increase cost-transparency. Recent legislation requires hospitals to publish a chargemaster, a list of all billable procedure codes together with prices. Chargemaster prices have been shown to be highly variable, if available, and are not typically paid, but contribute to negotiated rates. Read More

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Construction of a magnetic covalent organic framework with synergistic affinity strategy for enhanced glycopeptide enrichment.

J Mater Chem B 2021 Jul 23. Epub 2021 Jul 23.

National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, P. R. China.

Considering the inherent properties of glycopeptides, such as glycan structure, size, and hydrophilicity, affinity materials possessing suitable functional molecule-glycan interactions, matched channels with size exclusion, and surfaces with hydrophilic interactions are preferred for glycopeptide separation in biological samples. Here, a novel boronic-acid-functionalized magnetic covalent organic framework was prepared through epitaxial growth and multi-ligand strategies. The multi-ligand strategy was firstly employed to prepare functionalized magnetic covalent organic framework without any post-functionalization protocol. Read More

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Application of tandem fast protein liquid chromatography to purify intact native monomeric/aggregated Tamm-Horsfall protein from human urine and systematic comparisons with diatomaceous earth adsorption and salt precipitation: yield, purity and time-consumption.

Anal Methods 2021 Jul 23. Epub 2021 Jul 23.

Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, 6th Floor - SiMR Building, 2 Wanglang Road, Bangkoknoi, Bangkok 10700, Thailand.

Tamm-Horsfall protein (THP) is a high-abundance urinary protein. Although its functions have been studied for years, several aspects of these remain unclear. To achieve more knowledge on THP functions, an effective isolation/purification method providing a high yield and high purity is required. Read More

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US Sick Leave In Global Context: US Eligibility Rules Widen Inequalities Despite Readily Available Solutions.

Health Aff (Millwood) 2021 Jul 26:101377hlthaff202100731. Epub 2021 Jul 26.

Amy Raub is a principal research analyst at the WORLD Policy Analysis Center, UCLA.

Research has demonstrated that paid sick leave reduces spread of COVID-19 and other infectious diseases and improves preventive care and access to treatment across a wide range of conditions. However, the U.S. Read More

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A glutaminyl cyclase-catalyzed α-synuclein modification identified in human synucleinopathies.

Acta Neuropathol 2021 Jul 26. Epub 2021 Jul 26.

Paul Flechsig Institute for Brain Research, University of Leipzig, Liebigstraße 19, 04103, Leipzig, Germany.

Parkinson's disease (PD) is a progressive neurodegenerative disorder that is neuropathologically characterized by degeneration of dopaminergic neurons of the substantia nigra (SN) and formation of Lewy bodies and Lewy neurites composed of aggregated α-synuclein. Proteolysis of α-synuclein by matrix metalloproteinases was shown to facilitate its aggregation and to affect cell viability. One of the proteolysed fragments, Gln79-α-synuclein, possesses a glutamine residue at its N-terminus. Read More

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Systematic process evaluation of the conjugation of proteins to gold nanoparticles.

Heliyon 2021 Jun 25;7(6):e07392. Epub 2021 Jun 25.

Laboratorio de Biomateriales, Instituto de Química Biológica, Facultad de Ciencias, Universidad de la República, 11400, Montevideo, Uruguay.

The present work addresses some fundamental aspects in the preparation of protein-conjugated gold nanoparticles, in order to ensure an appropriate final product. Ten broadly available and/or easy to implement analytical tools were benchmarked and compared in their capacity to provide reliable and conclusive information for each step of the procedure. These techniques included transmission electron microscopy, UV/VIS spectroscopy, dynamic light scattering, zeta-potential, Fourier-transformed infrared spectroscopy, colloidal stability titration, end-point colloidal stability analysis, cyclic voltammetry, agarose gel electrophoresis and size-exclusion chromatography (SEC). Read More

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MRI-Based Morphometric Study Regarding Operative Windows of Oblique Lumbar Interbody Fusion in Indian Population.

Indian J Orthop 2021 Jul 7;55(Suppl 2):366-373. Epub 2021 Apr 7.

Department of Orthopaedics, School of Medical Sciences and Research, Sharda University, Greater Noida, Uttar Pradesh India.

Background: The novel Oblique lumbar interbody fusion [OLIF] technique has been proposed as a solution to approach related complications of anterior lumbar interbody fusion [ALIF] and lateral lumbar interbody fusion [LLIF]. There exists no study concerning morphological evaluation of retroperitoneal oblique corridor for the Oblique lumbar interbody fusion (OLIF) technique in the Indian population. The aim of our study was (a) to measure magnetic resonance imaging (MRI) based anatomic parameters concerning OLIF operative windows from L2-L3 to L4-L5 level (b) to determine the feasibility of this technique following MRI-based morphometric evaluation in the Indian population. Read More

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Investigation of the consequences of ultrasound on the physicochemical, emulsification, and gelatinization characteristics of citric acid-treated whey protein isolate.

J Dairy Sci 2021 Jul 22. Epub 2021 Jul 22.

Key Laboratory of Dairy Science, Northeast Agricultural University, Ministry of Education, Harbin, 150030, PR China. Electronic address:

The effect of ultrasound (US) pretreatment (0, 200, 400, 600, and 800 W) on the physicochemical, emulsification, and gelatinization characteristics of citric acid (CA)-treated whey protein isolate (WPI) was investigated. Size exclusion chromatography demonstrated that when compared with untreated WPI, US pretreatment promoted production of more molecular polymers in the CA-treated WPI. There was a reduction in particle size of CA-treated WPI with the increase of US power (0-800 W), whereas its free sulfhydryl content, surface hydrophobicity, and intrinsic fluorescence strength increased. Read More

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Breakthrough in polyurethane bio-recycling: An efficient laccase-mediated system for the degradation of different types of polyurethanes.

Waste Manag 2021 Jul 22;132:23-30. Epub 2021 Jul 22.

BioTeam/ICPEES-ECPM, UMR CNRS 7515, Université de Strasbourg, 25 rue Becquerel, 67087 Strasbourg Cedex 2, France. Electronic address:

Development of green, efficient and profitable recycling processes for plastic material will contribute to reduce the expanding plastic pollution and microplastics accumulation in the environment. Polyurethanes (PU) are versatile polymers with a large range of chemical compositions and structures. This variability increases the complexity of PU waste management. Read More

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Study on the assessment of humification processes during biodegradation of heavy residual fuel oil.

Sci Total Environ 2021 Jul 17;797:149099. Epub 2021 Jul 17.

BREM GROUP Ltd., Oslobođenja 39b, 11090 Belgrade, Serbia.

The aim of this study was to investigate the creation of humic substances during biodegradation of heavy residual fuel oil, because there are indications that substances similar to humic substances are generated during biodegradation of polycyclic aromatic hydrocarbons. In the study, which lasted for 110 days, biodegradation of heavy residual fuel oil was carried out in a layer of artificial soil substrate. The initial concentration of the total petroleum hydrocarbon in the prepared artificial soil substrate (biopile) was 23. Read More

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Protein structure and analysis single-chain Fv antibody covalently linked to antigen peptides and its structural evaluation.

Anal Biochem 2021 Jul 21:114312. Epub 2021 Jul 21.

Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, 1-5 Hangi-cho, Shimogamo, Sakyo-ku, Kyoto, Kyoto, 606-8522, Japan. Electronic address:

The monoclonal antibody G2 specifically recognizes different peptides. The single-chain Fv (scFv) antibodies of G2 covalently linked to antigen peptides, Pep18mer and Pep395, via a flexible linker were expressed in Escherichia coli in the insoluble fraction, and were solubilized using guanidine HCl, followed by refolding. We analyzed the folding thermodynamics of the refolded proteins, purified as monomers using size-exclusion chromatography (SEC). Read More

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Short Term Outcomes of Distal Extended EndoVascular Aortic Repair (DEEVAR) Petticoat in Acute and Subacute Complicated Type B Aortic Dissection.

Eur J Vasc Endovasc Surg 2021 Jul 20. Epub 2021 Jul 20.

Division of Vascular Surgery, Department of Cardiovascular Diseases, A. Manzoni Hospital, Lecco, Italy.

Objective: The aim of this study was to evaluate the peri-operative and short term results of institutional experience with the Distal Extended EndoVascular Aortic Repair PETTICOAT (DEEVAR PETTICOAT) procedure.

Methods: This was a single centre, observational study. From January 2015 to December 2019, 53 patients were admitted to the institution for treatment of acute and subacute complicated type B aortic dissection. Read More

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Photobiomodulation by Near-Infrared 980-nm Wavelengths Regulates Pre-Osteoblast Proliferation and Viability through the PI3K/Akt/Bcl-2 Pathway.

Int J Mol Sci 2021 Jul 15;22(14). Epub 2021 Jul 15.

Department of Surgical and Diagnostic Sciences, University of Genoa, 16132 Genoa, Italy.

Background: bone tissue regeneration remains a current challenge. A growing body of evidence shows that mitochondrial dysfunction impairs osteogenesis and that this organelle may be the target for new therapeutic options. Current literature illustrates that red and near-infrared light can affect the key cellular pathways of all life forms through interactions with photoacceptors within the cells' mitochondria. Read More

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Selenoproteome Expression Studied by Non-Radioactive Isotopic Selenium-Labeling in Human Cell Lines.

Int J Mol Sci 2021 Jul 7;22(14). Epub 2021 Jul 7.

Centre International de Recherche en Infectiologie (CIRI), 69007 Lyon, France.

Selenoproteins, in which the selenium atom is present in the rare amino acid selenocysteine, are vital components of cell homeostasis, antioxidant defense, and cell signaling in mammals. The expression of the selenoproteome, composed of 25 selenoprotein genes, is strongly controlled by the selenium status of the body, which is a corollary of selenium availability in the food diet. Here, we present an alternative strategy for the use of the radioactive Se isotope in order to characterize the selenoproteome regulation based on (i) the selective labeling of the cellular selenocompounds with non-radioactive selenium isotopes (Se, Se) and (ii) the detection of the isotopic enrichment of the selenoproteins using size-exclusion chromatography followed by inductively coupled plasma mass spectrometry detection. Read More

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The Lipid Composition of Serum-Derived Small Extracellular Vesicles in Participants of a Lung Cancer Screening Study.

Cancers (Basel) 2021 Jul 8;13(14). Epub 2021 Jul 8.

Maria Sklodowska-Curie National Research Institute of Oncology, Gliwice Branch, 44-102 Gliwice, Poland.

Molecular components of exosomes and other classes of small extracellular vesicles (sEV) present in human biofluids are potential biomarkers with possible applicability in the early detection of lung cancer. Here, we compared the lipid profiles of serum-derived sEV from three groups of lung cancer screening participants: individuals without pulmonary alterations, individuals with benign lung nodules, and patients with screening-detected lung cancer (81 individuals in each group). Extracellular vesicles and particles were purified from serum by size-exclusion chromatography, and a fraction enriched in sEV and depleted of low-density lipoproteins (LDLs) was selected (similar sized vesicles was observed in all groups: 70-100 nm). Read More

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Purification and HDL-like particle formation of apolipoprotein A-I after co-expression with the EDDIE mutant of Npro autoprotease.

Protein Expr Purif 2021 Jul 21;187:105946. Epub 2021 Jul 21.

Department of Biochemistry and Structural Biology, Lund University, P O Box 124, SE22100, Lund, Sweden. Electronic address:

Apolipoprotein A-I (ApoA-I) is the major protein constituent of high-density lipoprotein particles, and as such is involved in cholesterol transport and activation of LCAT (the lecithin:cholesterol acyltransferase). It may also form amyloidal deposits in the body, showing the multifaceted interactions of ApoA-I. In order to facilitate the study of ApoA-I in various systems, we have developed a protocol based on recombinant expression in E. Read More

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Ensemble Reduced Density Matrix Functional Theory for Excited States and Hierarchical Generalization of Pauli's Exclusion Principle.

Phys Rev Lett 2021 Jul;127(2):023001

CNR-Istituto Nanoscienze, Via Campi 213A, I-41125 Modena, Italy.

We propose and work out a reduced density matrix functional theory (RDMFT) for calculating energies of eigenstates of interacting many-electron systems beyond the ground state. Various obstacles which historically have doomed such an approach to be unfeasible are overcome. First, we resort to a generalization of the Ritz variational principle to ensemble states with fixed weights. Read More

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Biochemical and Phylogenetic Characterization of a Novel NADP-Specific Isocitrate Dehydrogenase From the Marine Microalga .

Front Mol Biosci 2021 5;8:702083. Epub 2021 Jul 5.

Anhui Provincial Key Laboratory of Molecular Enzymology and Mechanism of Major Diseases, Key Laboratory of Biomedicine in Gene Diseases and Health of Anhui Higher Education Institutes, College of Life Sciences, Anhui Normal University, Wuhu, China.

Isocitrate dehydrogenase (IDH) family of proteins is classified into three subfamilies, namely, types I, II, and III. Although IDHs are widely distributed in bacteria, archaea, and eukaryotes, all type III IDHs reported to date are found only in prokaryotes. Herein, a novel type III IDH subfamily member from the marine microalga (PtIDH2) was overexpressed, purified, and characterized in detail for the first time. Read More

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Structural Features of Cork Dioxane Lignin from L.

J Agric Food Chem 2021 Jul 21. Epub 2021 Jul 21.

CICECO, Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal.

The dioxane lignin was isolated from extractives- and suberin-free cork ( L.) by a modified acidolytic procedure and submitted to structural analysis by permanganate oxidation, analytical pyrolysis coupled with gas chromatography and mass spectrometry (Py-GC-MS), liquid- and solid-state nuclear magnetic resonance (NMR) spectroscopy, and Fourier transform infrared (FTIR) spectroscopy. The molecular weight ( = 2500 Da) was assessed by size exclusion chromatography (SEC). Read More

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Purification of RNA Mango Tagged Native RNA-protein Complexes from Cellular Extracts Using TO1-Desthiobiotin Fluorophore Ligand.

Bio Protoc 2018 Apr 5;8(7):e2799. Epub 2018 Apr 5.

Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada.

A native purification strategy using RNA Mango for RNA based purification of RNA-protein complexes is described. The RNA Mango aptamer is first genetically engineered into the RNA of interest. RNA Mango containing complexes obtained from cleared cellular native extracts are then immobilized onto TO1-Desthiobiotin saturated streptavidin agarose beads. Read More

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