142,039 results match your criteria situ hybridization


Eya1 protein distribution during embryonic development of Xenopus laevis.

Gene Expr Patterns 2021 Sep 15:119213. Epub 2021 Sep 15.

School of Natural Sciences, National University of Galway, Galway, Ireland. Electronic address:

Eya1 and other Eya proteins are important regulators of progenitor proliferation, cell differentiation and morphogenesis in all three germ layers. At present, most of our knowledge of Eya1 distribution is based on in situ hybridization for Eya1 mRNA. However, to begin to dissect the mechanisms underlying Eya1 functions, we need a better understanding of the spatiotemporal distribution of Eya1 proteins during embryonic development, their subcellular localization and their levels of expression in various tissues. Read More

View Article and Full-Text PDF
September 2021

Immunomodulatory role of short neuropeptide F in the mud crab Scylla paramamosain.

Dev Comp Immunol 2021 Sep 15:104260. Epub 2021 Sep 15.

College of Fisheries, Jimei University, Xiamen 361021, China. Electronic address:

Short neuropeptide F (sNPF) is bioactive peptide secreted by neurons of invertebrates. It is one of the important pleiotropic neural molecules that is associated with a variety of physiological processes in invertebrates. However, little is known about the role of sNPF in the immune response. Read More

View Article and Full-Text PDF
September 2021

Appropriate Use Criteria (AUC) for Ancillary Diagnostic Testing in Dermatopathology: New Recommendations for 11 tests and 220 clinical scenarios from the American Society of Dermatopathology AUC Committee.

J Cutan Pathol 2021 Sep 18. Epub 2021 Sep 18.

Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, Texas.

Background: Appropriate use criteria (AUC) provide patient-centered physician guidance in test selection. An initial set of AUC were reported by the American Society of Dermatopathology (ASDP) in 2018. AUC reflect evidence collected at single timepoints and may be affected by evolving evidence and experience. Read More

View Article and Full-Text PDF
September 2021

The hedgehog signaling pathway is expressed in the adult mouse hypothalamus and modulated by fasting.

eNeuro 2021 Sep 16. Epub 2021 Sep 16.

Department of Biology, Indiana University-Purdue University Indianapolis, Indianapolis, IN 46202 USA

The hedgehog signaling pathway is best known for its role in developmental patterning of the neural tube and limb bud. More recently, hedgehog signaling has been recognized for its roles in growth of adult tissues and maintenance of progenitor cell niches. However, the role of hedgehog signaling in fully differentiated cells like neurons in the adult brain is less clear. Read More

View Article and Full-Text PDF
September 2021

Clinicopathological features of Epstein-Barr virus-associated superficial early stage gastric cancer treated with endoscopic submucosal dissection.

Dig Liver Dis 2021 Sep 14. Epub 2021 Sep 14.

Department of Gastroenterology, Toranomon Hospital, Tokyo, Japan.

Background: Epstein-Barr virus (EBV) is known to be involved in gastric carcinogenesis. EBV-associated early gastric carcinoma (EBVEGC) has a lower incidence of lymph node involvement and could be an expanded indication for endoscopic submucosal dissection (ESD) treatment.

Aim: To clarify the prevalence and clinicopathological features of EBVEGC. Read More

View Article and Full-Text PDF
September 2021

Histologic and Molecular Characterization of Non-Small Cell Lung Carcinoma With Discordant ROS1 Immunohistochemistry and Fluorescence In Situ Hybridization.

Appl Immunohistochem Mol Morphol 2021 Sep 16. Epub 2021 Sep 16.

University of Utah and ARUP Laboratories, Salt Lake City, UT St. Jude Childrens' Research Hospital, Memphis, TN.

Introduction: ROS1 immunohistochemical (IHC) positivity requires follow-up with confirmatory testing such as fluorescence in situ hybridization (FISH). Identifying predictive characteristics of false positive ROS1 IHC cases could aid in optimizing testing algorithms, decrease testing costs and preserve tissue.

Materials And Methods: Retrospective results were retrieved for 2054 patients with non-small cell lung carcinoma submitted to our laboratory for molecular testing. Read More

View Article and Full-Text PDF
September 2021

Identification of variant APL translocations PRKAR1A-RARα and ZBTB16-RARα (PLZF-RARα) through the MI-ONCOSEQ platform.

Cancer Genet 2021 Aug 25;258-259:57-60. Epub 2021 Aug 25.

Division of Hematology and Medical Oncology, Department of Internal Medicine, Michigan Medicine, Ann Arbor, Michigan, USA. Electronic address:

The cornerstone of management in patients with acute promyelocytic leukemia (APL) is early diagnosis and prompt initiation of treatment with an all-trans retinoic acid (ATRA)-based regimen. Identification of the t(15;17)(PML-RARA) chromosomal translocation through conventional cytogenetics fluorescence in-situ hybridization (FISH) or detection of the promyelocytic leukemia-retinoic acid receptor alpha (PML-RARα) fusion through RT-PCR represent the current standard of care for diagnosing APL. However, about 1-2% of patients with APL have a variant translocation involving other fusion partners with RARα besides PML. Read More

View Article and Full-Text PDF

Performance of enhancement on brain MRI for identifying HER2 overexpression in breast cancer brain metastases.

Eur J Radiol 2021 Sep 11;144:109948. Epub 2021 Sep 11.

Department of Radiology, City of Hope Comprehensive Cancer Center, 1500 E. Duarte Rd, Duarte, CA 91010, USA.

Purpose: To investigate whether enhancement on MRI could help identify HER2 overexpression in breast cancer brain metastases.

Methods: We derived a cohort of 38 histologically proven breast cancer brain metastases with preoperative contrast-enhanced brain MRI and HER2 fluorescent in-situ hybridization of the resected/biopsied brain specimens from 2018 to 2021. Enhancement of the lesions was measured and compared using t-tests. Read More

View Article and Full-Text PDF
September 2021

Early-Onset Cerebral Amyloid Angiopathy and Alzheimer Disease Related to an APP Locus Triplication.

Neurol Genet 2021 Oct 8;7(5):e609. Epub 2021 Sep 8.

Department of Neurology and CNR-MAJ (L.G., D.W.), Normandie University, UNIROUEN, Inserm U1245, CHU Rouen, CIC-CRB1404, F 76000; Department of Genetics and CNR-MAJ (K.C., S.R., N.L.M., A.R.-L., D.C., G.N.), Normandie University, UNIROUEN, Inserm U1245 and CHU Rouen, F 76000; Department of Neurology (B.C., M.F.), Lyon University Hospital; Department of Neurology (O.M.), Grenoble University Hospital; Department of Histology (J.B.), Grenoble University Hospital; AP-HP (M.M., T.C., E.T.-L.), Groupe Hospitalier Saint-Louis Lariboisière-Fernand-Widal, Service de Génétique Moléculaire Neurovasculaire, INSERM UMR 1141, NeuroDiderot,Université de Paris; Department of Histology Embryology and Cytogenetics (E.P.), Jean Verdier Hospital; Paris 13 University (E.P.), Sorbonne Paris Cité, UFR SMBH Bobigny; and PROTECT (E.P.), INSERM, Paris Diderot University, Bondy, France.

Background And Objective: To report a triplication of the amyloid-β precursor protein () locus along with relative messenger RNA (mRNA) expression in a family with autosomal dominant early-onset cerebral amyloid angiopathy (CAA) and Alzheimer disease (AD).

Methods: Four copies of the gene were identified by quantitative multiplex PCR of short fluorescent fragments, fluorescent in situ hybridization (FISH), and array comparative genomic hybridization. mRNA levels were assessed using reverse-transcription-digital droplet PCR in the proband's whole blood and compared with 10 controls and 9 duplication carriers. Read More

View Article and Full-Text PDF
October 2021

Candidate oncogene circularNOP10 mediates gastric cancer progression by regulating miR-204/SIRT1 pathway.

J Gastrointest Oncol 2021 Aug;12(4):1428-1443

Department of Pathology, School of Medicine, Southeast University, Nanjing, China.

Background: The role of circular RNA (circRNA) in gastric cancer (GC) is attracting increasing attention. CircNOP10 (hsa_circ-0034351) has been reported to be upregulated in human GC tissue. However, the biological role and mechanism of circNOP10 in GC remain unknown. Read More

View Article and Full-Text PDF

The relationship between the number of circulating tumor cells and the prognosis in patients with esophageal squamous cell carcinoma.

J Gastrointest Oncol 2021 Aug;12(4):1265-1276

Department of Thoracic Surgery, First Medical Center, General Hospital of Chinese PLA, Beijing, China.

Background: Esophageal cancer (EC) is one of the most common malignancies worldwide, with high morbidity and mortality rates. Circulating tumor cell (CTC) detection has become a novel approach in clinical study of EC. In this study, the relationship between CTCs/c-Kit expression of CTCs and the prognosis of EC patients was analyzed in EC. Read More

View Article and Full-Text PDF

TLR7 is expressed by support cells, but not sensory neurons, in ganglia.

J Neuroinflammation 2021 Sep 16;18(1):209. Epub 2021 Sep 16.

Division of Pulmonary and Critical Care Medicine, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, UHN67, Portland, OR, 97239, USA.

Background: Toll-like receptor 7 (TLR7) is an innate immune receptor that detects viral single-stranded RNA and triggers the production of proinflammatory cytokines and type 1 interferons in immune cells. TLR7 agonists also modulate sensory nerve function by increasing neuronal excitability, although studies are conflicting whether sensory neurons specifically express TLR7. This uncertainty has confounded the development of a mechanistic understanding of TLR7 function in nervous tissues. Read More

View Article and Full-Text PDF
September 2021

Pathological cytomorphologic features and the percentage of ALK FISH-positive cells predict pulmonary adenocarcinoma prognosis: a prospective cohort study.

World J Surg Oncol 2021 Sep 16;19(1):278. Epub 2021 Sep 16.

Department of Oncology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, 264000, People's Republic of China.

Background: We conducted a study to explore the relationship between pathological cytomorphologic features and the percentage of anaplastic lymphoma kinase (ALK)-positive cells to better predict pulmonary adenocarcinoma prognosis with crizotinib treatment.

Patients And Methods: We investigated 60 cases of patients with ALK-positive advanced or metastatic non-small cell lung cancer (NSCLC). Immunohistochemistry was performed to screen for ALK rearrangement. Read More

View Article and Full-Text PDF
September 2021

Recurrent Chromosomal Abnormalities in Tissues Involved by Chronic Lymphocytic Leukemia/Small Lymphocytic Lymphoma.

Am J Clin Pathol 2021 Sep 16. Epub 2021 Sep 16.

Division of Laboratory Genetics and Genomics, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.

Objectives: Prognostically relevant chromosomal abnormalities in chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) are routinely identified by fluorescence in situ hybridization (FISH) on peripheral blood or bone marrow specimens. We studied the prevalence of chromosomal abnormalities on extramedullary tissues involved by CLL/SLL and evaluated their association with prominent proliferation centers (PPCs).

Methods: FISH for recurrent abnormalities in CLL/SLL was performed on formalin-fixed, paraffin-embedded biopsy sections. Read More

View Article and Full-Text PDF
September 2021

Cholangioscopy in primary sclerosing cholangitis: a case series of benign features.

VideoGIE 2021 Sep 30;6(9):431-437. Epub 2021 Jun 30.

Department of Pathology and Laboratory Medicine, University of California Davis School of Medicine, Sacramento, California.

Background And Aims: Cholangioscopy is useful in establishing a visual diagnosis of cholangiocarcinoma (CCA), but this is harder to achieve in primary sclerosing cholangitis (PSC) because of the stricture-forming nature of the disease. Furthermore, it can be harder to differentiate malignant from benign features of the underlying inflammation. This case series demonstrates the varied features of nonmalignant inflammatory findings in PSC. Read More

View Article and Full-Text PDF
September 2021

circHMGCS1-016 reshapes immune environment by sponging miR-1236-3p to regulate CD73 and GAL-8 expression in intrahepatic cholangiocarcinoma.

J Exp Clin Cancer Res 2021 Sep 15;40(1):290. Epub 2021 Sep 15.

Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, China.

Background: Accumulating evidence indicates that circRNAs may serve as essential regulators in the progression of several human cancers, but the function and mechanism of circRNAs in intrahepatic cholangiocarcinoma (ICC) are largely unknown.

Methods: RNA-seq was used to assess differentially expressed circRNAs between 4 ICC and peritumor tissues. Quantitative RT-PCR and in situ hybridization were used to determine the circHMGCS1-016 expression in ICC tissues. Read More

View Article and Full-Text PDF
September 2021

Methods for actionable gene fusion detection in lung cancer: now and in the future.

Pharmacogenomics 2021 Sep 16. Epub 2021 Sep 16.

Department of Public Health, University of Naples Federico II, Naples, Italy.

Although gene fusions occur rarely in non-small-cell lung cancer (NSCLC) patients, they represent a relevant target in treatment decision algorithms. To date, immunohistochemistry and fluorescence hybridization are the two principal methods used in clinical trials. However, using these methods in routine clinical practice is often impractical and time consuming because they can only analyze single genes and the quantity of tissue material is often insufficient. Read More

View Article and Full-Text PDF
September 2021

CIC-NUTM1 Sarcomas Affecting the Spine: A Subset of CIC-Rearranged Sarcomas Commonly Present in the Axial Skeleton.

Arch Pathol Lab Med 2021 Sep 15. Epub 2021 Sep 15.

From the Department of Pathology, School of Basic Medical Sciences, Third Hospital, Peking University Health Science Center, Beijing, China (Yang, Zhang).

Context.—: Tumors harboring CIC-NUTM1 fusion are a newly recognized rare sarcoma, but the documented cases are still limited. It is unclear whether it is the same as classic CIC-DUX4 sarcoma in terms of its clinical, pathologic, and behavioral aspects. Read More

View Article and Full-Text PDF
September 2021

Cloning and characterization of Thioredoxin 1 from the Cnidarian Hydra.

J Biochem 2021 Sep 15. Epub 2021 Sep 15.

Department of Zoology, Savitribai Phule Pune University, Pune 411007, India.

Thioredoxins, small disulphide-containing redox proteins, play an important role in the regulation of cellular thiol redox balance through their disulfide reductase activity. In this study, we have identified, cloned, purified and characterized thioredoxin 1 (HvTrx1) from the Cnidarian Hydra vulgaris Ind-Pune. Bioinformatics analysis revealed that HvTrx1 contains an evolutionarily conserved catalytic active site CGPC and shows a closer phylogenetic relationship with vertebrate Trx1. Read More

View Article and Full-Text PDF
September 2021

Lessons learnt from MDM2 FISH analysis on 439 mature lipomatous lesions with emphasis on atypical lipomatous tumours / well-differentiated liposarcoma (ALT/WDLPS) lacking cytological atypia.

Histopathology 2021 Sep 14. Epub 2021 Sep 14.

Department of Anatomical Pathology, Sonic Healthcare - Douglass Hanly Moir Pathology, Macquarie Park, Australia.

Aims: MDM2 gene amplification, usually detected by fluorescence in situ hybridization (FISH), is the key driving event in atypical lipomatous tumours/well-differentiated liposarcomas (ALT/WDL). We sought to determine the concordance between the histopathological findings and MDM2 FISH in the diagnosis of ALT/WDL and to identify the histological features of MDM2 amplified tumours lacking classic atypia.

Methods And Results: We performed a retrospective analysis of all mature lipomatous lesions subjected to MDM2 FISH analysis at our institution. Read More

View Article and Full-Text PDF
September 2021

Placental Swab in Supporting Diagnosis of Vertical Transmission in SARS-CoV-2 Positive Mothers.

Ir Med J 2021 Aug 19;114(7):409. Epub 2021 Aug 19.

Department of Neonatology, University Maternity Hospital Limerick, Limerick, Ireland.

Aims To review the evidence regarding the possibility of fetal vertical transmission in COVID-19 positive pregnant mothers by diagnosing through placental swabs. Methods The search terms 'pregnant COVID-19 positive mothers', 'fetal vertical transmission' and 'placental swabs' were used. 20 papers were selected. Read More

View Article and Full-Text PDF

High-throughput kinome-RNAi screen identifies protein kinase R activator (PACT) as a novel genetic modifier of CUG foci integrity in myotonic dystrophy type 1 (DM1).

PLoS One 2021 14;16(9):e0256276. Epub 2021 Sep 14.

Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.

Myotonic Dystrophy Type 1 (DM1) is the most common form of adult muscular dystrophy (~1:8000). In DM1, expansion of CTG trinucleotide repeats in the 3' untranslated region of the dystrophia myotonica protein kinase (DMPK) gene results in DMPK mRNA hairpin structures which aggregate as insoluble ribonuclear foci and sequester several RNA-binding proteins. The resulting sequestration and misregulation of important splicing factors, such as muscleblind-like 1 (MBNL1), causes the aberrant expression of fetal transcripts for several genes that contribute to the disease phenotype. Read More

View Article and Full-Text PDF
September 2021

HER2-Low Breast Cancers.

Am J Clin Pathol 2021 Sep 14. Epub 2021 Sep 14.

Department of Pathology, University of Rochester Medical Center, Rochester, NY, USA.

Objectives: Recent clinical trials have demonstrated significant clinical benefits from novel therapeutic compounds in breast cancer patient with human epidermal growth factor receptor 2 (HER2) immunohistochemical (IHC) score of 1+ or 2+ and negative in situ hybridization (ISH) result. A new concept of "HER2-low" breast cancer has been proposed and applied in the recent and ongoing clinical trials. In this article, we review the literature on the topic of HER2-low breast cancer. Read More

View Article and Full-Text PDF
September 2021

Primary cutaneous adenoid cystic carcinoma: A Clinicopathologic, Immunohistochemical, and fluorescence in-situ hybridization Study of 13 Cases.

Histopathology 2021 Sep 13. Epub 2021 Sep 13.

Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, People's Republic of China.

Aims: The study aimed to investigate the clinical, histological, immunohistochemical, and chromosomal features of primary cutaneous adenoid cystic carcinoma (PCACC).

Methods And Results: We retrospectively analyzed 13 cases identified on their clinicopathological features and performed fluorescence in situ hybridization (FISH) on 6 available cases. Head and neck (46. Read More

View Article and Full-Text PDF
September 2021

Genetic predisposition to tinnitus in the UK Biobank population.

Sci Rep 2021 Sep 13;11(1):18150. Epub 2021 Sep 13.

Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE, USA.

Tinnitus, the phantom perception of noise originating from the inner ear, has been reported by 15% of the world's population, with many patients reporting major deficits to cognition and mood. However, both objective diagnostic tools and targeted therapeutic strategies have yet to be established. To better understand the underlying genes that may preclude tinnitus, we performed a genome-wide association study of the UK Biobank's 49,960 whole exome sequencing participants to identify any loci strongly associated with tinnitus. Read More

View Article and Full-Text PDF
September 2021

A rare case of myxoid pleomorphic liposarcoma in an infant: A report.

Int J Surg Case Rep 2021 Sep 6;87:106365. Epub 2021 Sep 6.

Department of Cardiothoracic and Vascular Surgery, Manmohan Cardiothoracic Vascular and Transplant Center, Institute of Medicine, Kathmandu, Nepal.

Introduction And Importance: MPL (myxoid pleomorphic liposarcoma) is an uncommon type of liposarcoma that affects mostly children and infants. Its aggressive behavior and tendency to recur warrant complete excision despite the challenges of troublesome locations.

Case Presentation: A 12-month-old infant presented with an insidious onset of noisy breathing and respiratory distress not relieved by supplemental oxygen via face mask. Read More

View Article and Full-Text PDF
September 2021

Analysis of the gut microbiome in dogs and cats.

Vet Clin Pathol 2021 Sep 12. Epub 2021 Sep 12.

Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, USA.

The gut microbiome is an important immune and metabolic organ. Intestinal bacteria produce various metabolites that influence the health of the intestine and other organ systems, including kidney, brain, and heart. Changes in the microbiome in diseased states are termed dysbiosis. Read More

View Article and Full-Text PDF
September 2021

What Would Next Generation Sequencing Bring to the Diagnosis and Treatment of Sarcomas? A Series of 20 Cases, a Single Institution's Experience.

Turk Patoloji Derg 2021 ;37(3):226-232

Department of Pathology, Koç University, School of Medicine, ISTANBUL, TURKEY.

Objective: Soft tissue tumors comprise a small proportion of a pathologist's routine practice. Although morphology and immunohistochemistry are quite helpful for diagnosing these tumors, many require molecular tests. Fluorescence in-situ hybridization has been the most commonly used method for the detection of specific genomic alteration, but next generation sequencing (NGS) could be more informative in many ways. Read More

View Article and Full-Text PDF
January 2021

Detection of ALK Gene Rearrangements in Non-Small Cell Lung Cancer by Immunocytochemistry and Fluorescence in Situ Hybridization on Cytologic Samples.

Turk Patoloji Derg 2021 Jun 26. Epub 2021 Jun 26.

Department of Pathology, Post Graduate Institute of Medical Education and Research, CHANDIGARH, INDIA.

Objective: Determination of the molecular status is mandatory for personalized treatment of patients with non-small cell lung carcinoma. The present study was performed to detect anaplastic lymphoma kinase (ALK) rearrangements in pulmonary adenocarcinoma on cytology samples, using immunocytochemistry (ICC) and fluorescence in situ hybridization (FISH) on cell-blocks to assess the diagnostic reliability of these two techniques.

Material And Method: A total of 50 confirmed lung adenocarcinoma cases were included. Read More

View Article and Full-Text PDF

Signaling Modulation by During Tooth Morphogenesis in Mice.

Front Cell Dev Biol 2021 25;9:697243. Epub 2021 Aug 25.

Department of Biochemistry, School of Dentistry, Kyungpook National University, Daegu, South Korea.

miRNAs are conserved short non-coding RNAs that play a role in the modulation of various biological pathways during tissue and organ morphogenesis. In this study, the function of in tooth development, through its loss or gain in function was evaluated. A variety of techniques were utilized to evaluate detailed functional roles of during odontogenesis, including tooth cultivation, renal capsule transplantation, hybridization, real-time PCR, and immunohistochemistry. Read More

View Article and Full-Text PDF