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A fluorescent and colorimetric dual-channel sensor based on acid phosphatase-triggered blocking of internal filtration effect.

Mikrochim Acta 2021 Aug 2;188(8):282. Epub 2021 Aug 2.

State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China.

A colorimetric and fluorescent dual-channel detection method for acid phosphatase (ACP) activity has been constructed, based on the internal filtering effect between oxidized 3,3',5,5'-tetramethylbenzidine (oxTMB) and rhodamine B (RB). Au, which in situ form gold nanoparticles (AuNPs), can oxidize colorless 3,3',5,5'-tetramethylbenzidine (TMB) to oxTMB (blue color). The fluorescence of RB can be quenched by oxTMB due to the spectral overlap of emission of RB and absorption of oxTMB. Read More

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Simple and portable on-site system for nucleic acid-based detection of Clostridium difficile in stool samples using two columns containing microbeads and loop-mediated isothermal amplification.

Anal Bioanal Chem 2021 Aug 3. Epub 2021 Aug 3.

School of Integrative Engineering, Chung-Ang University, Seoul, 06974, South Korea.

It is challenging to employ nucleic acid-based diagnostics for the in situ detection of Clostridium difficile from complex fecal samples because essential sample preparation and amplification procedures require various experimental resources. In this study, a simple and effective on-site nucleic acid-based detection system was used to detect C. difficile in stool samples. Read More

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First report of leaf spot disease caused by on garlic in Korea.

Plant Dis 2021 Aug 2. Epub 2021 Aug 2.

National Institute of Horticultural & Herbal Science, Horticultural and Herbal Crop Environment Division, 100, Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do, Korea (the Republic of), 565-852;

Garlic ( L. cv.) is one of the most popular vegetables grown in Korea due to its high demand from the food industry. Read More

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Mutations in a β-group of solute carrier gene are responsible for egg and eye coloration of the brown egg 4 (b-4) mutant in the silkworm, Bombyx mori.

Insect Biochem Mol Biol 2021 Jul 29:103624. Epub 2021 Jul 29.

Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan. Electronic address:

The brown egg 4 (b-4) is a recessive mutant in the silkworm (Bombyx mori), whose egg and adult compound eyes exhibit a reddish-brown color instead of normal purple and black, respectively. By double digest restriction-site associated DNA sequencing (ddRAD-seq) analysis, we narrowed down a region linked to the b-4 phenotype to approximately 1.1 Mb that contains 69 predicted gene models. Read More

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Progress in Microdermal Grafting for Color Regeneration of White Scars.

Aesthet Surg J 2021 Jul 31. Epub 2021 Jul 31.

Division of Plastic and Reconstructive Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.

Background: Microdermal grafting is used to regenerate color in white (hypopigmented) scars using knife cutting partially de-epithelialized skin for grafting. However, the scalp has more melanocytes, and dermabrasion can preserve more melanocytes than knife cutting during partial de-epithelialization. Our clinics refined the two procedures that we originally reported using dermabrasion, not knife cutting, to partially de-epithelialize skin, and harvesting melanocyte donor sites from the scalp, not the skin. Read More

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Outcomes of the keystone perforator island flap for oncologic reconstruction of the back.

J Surg Oncol 2021 Jul 29. Epub 2021 Jul 29.

Division of Plastic and Reconstructive Surgery, Mayo Clinic, Rochester, Minnesota, USA.

Background: Coverage of posterior trunk defects after tumor resection can be challenging due to the intricate anatomy. The keystone perforator island flap (KPIF) provides coverage of the defect without the need for distant flap coverage or microsurgery, matches the recipient's skin color and contour, and requires a short operative time.

Methods: A retrospective review of all oncological back reconstructions with KPIF was performed at our institution. Read More

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Novel doublesex duplication associated with sexually dimorphic development of dogface butterfly wings.

Mol Biol Evol 2021 Jul 29. Epub 2021 Jul 29.

Department of Biological Sciences, Auburn University, USA.

Sexually dimorphic development is responsible for some of the most remarkable phenotypic variation found in nature. Alternative splicing of the transcription factor gene doublesex is a highly conserved developmental switch controlling the expression of sex specific pathways. Here, we leverage sex-specific differences in butterfly wing color pattern to characterize the genetic basis of sexually dimorphic development. Read More

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First Report of Lotus Seedpod Withering Caused by Fusarium proliferatum on Nelumbo nucifera Plants in China.

Plant Dis 2021 Jul 28. Epub 2021 Jul 28.

Jiangxi Agricultural University, 91595, College of Agronomy, Zhimin Road, Nanchang, China, 330045;

Nelumbo nucifera (Nymphaeaceae family) is a well-known plant in China and with the increasing value of this crop, the planting area of lotus is expanding. In May 2019, an unknown withering lotus seedpod was obtained in Guangchang County of Jiangxi Province (26.79°N, 116. Read More

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Test clamp procedure in robot-assisted partial nephrectomy: is it a safe procedure?

J Robot Surg 2021 Jul 27. Epub 2021 Jul 27.

Department of Urology, Kanazawa University Hospital, 13-1 Takaramachi, Kanazawa, Ishikawa, 920-8640, Japan.

We performed test clamp procedure in robot-assisted partial nephrectomy (RAPN) to prevent massive bleeding during tumor resection and to omit dissection of non-feeding arteries around the tumor. We subsequently analyzed the safety and usefulness of the procedure. The Test clamp procedure was performed for 1 to 3 min during renal artery test ischemia prior to the actual ischemia and tumor resection. Read More

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[Application of lateral circumflex femoral artery chimeric flap to repair complex wounds of foot and ankle].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2021 Jul;35(7):891-895

Department of Plastic Surgery and Burns, Affiliated Hospital of Zunyi Medical University, Zunyi Guizhou, 563000, P.R.China.

Objective: To investigate the effectiveness of lateral circumflex femoral artery chimeric flap transplantation in repair of complex wounds of foot and ankle.

Methods: A retrospective study was conducted to analyze the clinical data of 20 patients with complex wounds of foot and ankle treated with lateral circumflex femoral artery chimeric flap between June 2017 and June 2020. There were 14 males and 6 females with an average age of 42. Read More

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Social environment and genetics underlie body site-specific microbiomes of Yellowstone National Park gray wolves ().

Ecol Evol 2021 Jul 21;11(14):9472-9488. Epub 2021 Jun 21.

Ecology and Evolutionary Biology Princeton University Princeton NJ USA.

The host-associated microbiome is an important player in the ecology and evolution of species. Despite growing interest in the medical, veterinary, and conservation communities, there remain numerous questions about the primary factors underlying microbiota, particularly in wildlife. We bridged this knowledge gap by leveraging microbial, genetic, and observational data collected in a wild, pedigreed population of gray wolves () inhabiting Yellowstone National Park. Read More

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[Clinical effects of ulnar artery perforator chain flaps in repairing wounds on distal forearm or wrist with vascular anastomosis].

Zhonghua Shao Shang Za Zhi 2021 Jul;37(7):635-639

Department of Hand Surgery, the Second Hospital of Tangshan, Tangshan 063000, China.

To investigate the clinical effects of ulnar artery perforator chain flaps in repairing wounds on distal forearm or wrist with vascular anastomosis. The retrospective observational study method was used. From March 2015 to July 2019, a total of 11 serious trauma patients on distal forearm or wrist with vascular injury were admitted to the Second Hospital of Tangshan, including 8 male patients and 3 female patients, aged from 25 to 62 years, with an average age of 45 years. Read More

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Anatomic Distribution of Cherry Angiomas in the General Population.

Dermatology 2021 Jul 22:1-9. Epub 2021 Jul 22.

The University of Queensland Diamantina Institute, The University of Queensland, Dermatology Research Centre, Brisbane, Queensland, Australia.

Background: Cherry angiomas are common benign vascular skin lesions of unknown aetiology, found largely on the trunk. However, their exact anatomic distribution besides their truncal predisposition, and how they manifest in the general population, has not been characterised.

Methods: Three-dimensional (3D) total body imaging was obtained from 163 adult participants of a general population cohort study in Brisbane, Australia. Read More

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The impact of site-specific digital histology signatures on deep learning model accuracy and bias.

Nat Commun 2021 07 20;12(1):4423. Epub 2021 Jul 20.

Section of Hematology/Oncology, Department of Medicine, University of Chicago, Chicago, IL, USA.

The Cancer Genome Atlas (TCGA) is one of the largest biorepositories of digital histology. Deep learning (DL) models have been trained on TCGA to predict numerous features directly from histology, including survival, gene expression patterns, and driver mutations. However, we demonstrate that these features vary substantially across tissue submitting sites in TCGA for over 3,000 patients with six cancer subtypes. Read More

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Identification of QTL related to anther color and hull color by RAD sequencing in a RIL population of Setaria italica.

BMC Genomics 2021 Jul 20;22(1):556. Epub 2021 Jul 20.

Anyang Academy of Agriculture Sciences, 455000, Anyang, Henan, China.

Background: Foxtail millet (Setaria italica) is one of the oldest domesticated crops and has been considered as an ideal model plant for C grasses. It has abundant type of anther and hull colors which is not only a most intuitive morphological marker for color selection in seed production, but also has very important biological significance for the study of molecular mechanism of regulating the synthesis and metabolism of flavonoids and lignin. However, only a few genetic studies have been reported for anther color and hull color in foxtail millet. Read More

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The distribution and ecological effects of microplastics in an estuarine ecosystem.

Environ Pollut 2021 Jul 7;288:117731. Epub 2021 Jul 7.

Institute of Marine Science, University of Auckland, Private Bag 92019, Auckland, New Zealand.

Coastal sediments, where microplastics (MPs) accumulate, support benthic microalgae (BMA) that contribute to ecosystem functions such as primary production, nutrient recycling and sediment biostabilization. The potential interactions between MPs, BMA and associated properties and functions remain poorly understood. To examine these interactions, a survey of 22 intertidal sites was conducted. Read More

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Dual Color, Live Imaging of Vesicular Transport in Axons of Cultured Sensory Neurons.

Bio Protoc 2021 Jun 20;11(12):e4067. Epub 2021 Jun 20.

Neuroscience Institute, New York University Langone Medical Center, New York, USA.

The function of neurons in afferent reception, integration, and generation of electrical activity relies on their strikingly polarized organization, characterized by distinct membrane domains. These domains have different compositions resulting from a combination of selective targeting and retention of membrane proteins. In neurons, most proteins are delivered from their site of synthesis in the soma to the axon via anterograde vesicular transport and undergo retrograde transport for redistribution and/or lysosomal degradation. Read More

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Robust Yellow-Violet Pigments Tuned by Site-Selective Manganese Chromophores.

Inorg Chem 2021 Aug 14;60(15):11579-11590. Epub 2021 Jul 14.

Key Laboratory of Bioinorganic and Synthetic Chemistry of Ministry of Education, School of Chemistry, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.

The rational design of multifunctional inorganic pigments relies on the manipulation of ionic valence and local surroundings of a chromophore in structurally and chemically habitable hosts. To date, the development of environmentally benign and intense violet/purple pigments is still a challenge. Here we report a family of MnTeO and MnLiTeO ( = Zn, Mg; = 0. Read More

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US-guided high-volume injection for Achilles tendinopathy.

J Ultrason 2021 Jun 18;21(85):e127-e133. Epub 2021 Jun 18.

Department of Medical Imaging, University Hospital of Heraklion, Crete, Greece.

Achilles tendinopathy is a common overuse condition affecting the adult population. The incidence is on the rise because of greater participation of people in recreational or competitive sporting activities. Chronic Achilles tendinopathy occurs most commonly in the tendon's mid-portion, and it is challenging to manage, leading to significant patient morbidity. Read More

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Ecology and colour in 1m: a contemplative, place-based study.

Cult Stud Sci Educ 2021 Jul 8:1-19. Epub 2021 Jul 8.

Department of Biology, Kwantlen Polytechnic University, Surrey, BC Canada.

This essay argues the importance of interdisciplinary, contemplative, place-based pedagogy. The Ecology and Colour in 1m study has students from the sciences and the arts observe a small quadrat in their local community over several weeks, engaging in both scientific and creative expression. The connection to Aldo Leopold's teaching principles and its relevance during our current screen fatigue pandemic and increasing disconnection from the natural world are outlined. Read More

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ddPCR increases detection of SARS-CoV-2 RNA in patients with low viral loads.

Arch Virol 2021 Sep 12;166(9):2529-2540. Epub 2021 Jul 12.

Unité "Organisation nucléaire et Oncogenèse", INSERM U993, Institut Pasteur, 28, rue du Docteur Roux, 75724, Paris, Cedex 15, France.

RT-qPCR detection of SARS-CoV-2 RNA still represents the method of reference to diagnose and monitor COVID-19. From the onset of the pandemic, however, doubts have been expressed concerning the sensitivity of this molecular diagnosis method. Droplet digital PCR (ddPCR) is a third-generation PCR technique that is particularly adapted to detecting low-abundance targets. Read More

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September 2021

A Standardized Analysis of Tertiary Lymphoid Structures in Human Melanoma: Disease Progression- and Tumor Site-Associated Changes With Germinal Center Alteration.

Front Immunol 2021 24;12:675146. Epub 2021 Jun 24.

Laboratory of Molecular Dermato-Oncology and Tumor Immunology, Department of Dermatology, Medical University of Vienna, Vienna, Austria.

There is increasing evidence that tertiary lymphoid structures (TLS) control not only local adaptive B cell responses at melanoma tumor sites but also the cellular composition and function of other immune cells. In human melanoma, however, a comprehensive analysis of TLS phenotypes, density and spatial distribution at different disease stages is lacking. Here we used 7-color multiplex immunostaining of whole tissue sections from 103 human melanoma samples to characterize TLS phenotypes along the expression of established TLS-defining molecular and cellular components. Read More

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Open-source all-in-one LabToGo Office Chromatography.

Anal Chim Acta 2021 Aug 4;1174:338702. Epub 2021 Jun 4.

Chair of Food Science, Institute of Nutritional Science, and Interdisciplinary Research Center (iFZ), Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany. Electronic address:

Print and media technologies were used uncommonly in the field of chromatography and explored in application to create a miniaturized all-in-one LabToGo system. This novel research field termed Office Chromatography (OC) uses additive manufacturing in terms of 3D printing of operational parts as well as open-source hard- and software. The OCLab2 presented here has been considerably extended in its functionalities. Read More

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Genome wide association study of agronomic and seed traits in a world collection of proso millet (Panicum miliaceum L.).

BMC Plant Biol 2021 Jul 9;21(1):330. Epub 2021 Jul 9.

Institute of Life Sciences, Scuola Superiore Sant'Anna, Pisa, Italy.

Background: The climate crisis threatens sustainability of crop production worldwide. Crop diversification may enhance food security while reducing the negative impacts of climate change. Proso millet (Panicum milaceum L. Read More

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A novel ratiometric fluorescent chemosensor for detecting malononitrile and application assisted with smartphone.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Dec 1;262:120135. Epub 2021 Jul 1.

Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, College of Light Industry and Food, Nanjing Forestry University, Nanjing 210037, China. Electronic address:

Malononitrile can be transformed into hypertoxic hydrogen cyanide which induces severely jeopardizes to human beings and environment. However, an effective detection technology for malononitrile was still lacking, which means that it is necessary to develop new sensitive analysis technology for malononitrile. Here in, a high sensitive fluorescent probe NQBS for detecting malononitrile was designed and synthesized from the derivative of natural product nopinone. Read More

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December 2021

Site-specific generation of excited state wavepackets with high-intensity attosecond x rays.

J Chem Phys 2021 Jun;154(22):224111

Chemical Sciences and Engineering Division, Argonne National Laboratory, 9700 S Cass Avenue, Lemont, Illinois 60439, USA.

High-intensity attosecond x rays can produce coherent superpositions of valence-excited states through two-photon Raman transitions. The broad-bandwidth, high-field nature of the pulses results in a multitude of accessible excited states. Multiconfigurational quantum chemistry with the time-dependent Schrödinger equation is used to examine population transfer dynamics in stimulated x-ray Raman scattering of the nitric oxide oxygen and nitrogen K-edges. Read More

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Distribution of scatter radiation by C-arm cone-beam computed tomography in angiographic suite: measurement of doses and effectiveness of protection devices.

Nagoya J Med Sci 2021 May;83(2):277-286

Department of Radiology, Aichi Medical University, Nagakute, Japan.

Distribution of radiation by C-arm cone-beam computed tomography (CBCT) in the angiographic suite and effectiveness of protection devices were assessed. CBCT image of a human phantom was obtained by a rotation of 220 degrees during 8 seconds of exposure. One hundred and twelve dosimeters were placed at different positions around the beam entry site, and color maps of dose distributions were drawn for horizontal and vertical planes. Read More

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Fixed-Combination Halobetasol Propionate and Tazarotene Lotion for Psoriasis in Patients With Skin of Color.

J Drugs Dermatol 2021 Jul;20(7):744

Background: Few studies have examined topical psoriasis therapies in patients with skin of color. Fixed-combination halobetasol propionate (0.01%) and tazarotene (0. Read More

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Dual-mode aptasensor for simultaneous detection of multiple food-borne pathogenic bacteria based on colorimetry and microfluidic chip using stir bar sorptive extraction.

Mikrochim Acta 2021 Jul 6;188(8):244. Epub 2021 Jul 6.

Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo, 315211, China.

A dual-mode aptasensor using colorimetry and microfluidic chip (MC) together with stir bar sorptive extraction (SBSE) has been developed for firstly qualifying samples contaminated with Vibrio parahaemolyticus (V.P) and Salmonella typhimurium (S.T), then precisely determine both of them in positive samples. Read More

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Aesthetic reconstruction for syndactyly using the "gradation skin graft" from the plantar instep area.

J Plast Reconstr Aesthet Surg 2021 Jun 6. Epub 2021 Jun 6.

Department of Plastic and Reconstructive Surgery, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8577, Japan.

In syndactyly, the donor site of the skin graft should have the characteristics of both the dorsal and the plantar or palmar skin because the skin defects after the dividing procedure are at the lateral sides of the digits. The plantar instep region has a color and texture border between the dorsal and plantar skin as a gradational boundary belt. We performed "gradation skin grafting" including the belt for syndactyly. Read More

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