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Perfusion and Permeability MRI Predicts Future Cavernous Angioma Hemorrhage and Growth.

J Magn Reson Imaging 2021 Sep 24. Epub 2021 Sep 24.

Neurovascular Surgery Program, Department of Neurosurgery, University of Chicago Medicine and Biological Sciences, Chicago, Illinois, USA.

Background: Cerebral cavernous angioma (CA) is a capillary vasculopathy affecting more than a million Americans with a small fraction of cases demonstrating lesional bleed or growth with major clinical sequelae. Perfusion and permeability are fundamental features of CA pathophysiology, but their role as prognostic biomarkers is unclear.

Purpose: To investigate whether perfusion or permeability lesional descriptors derived from dynamic contrast-enhanced quantitative perfusion (DCEQP) magnetic resonance imaging (MRI) can predict subsequent lesional bleed/growth in the year following imaging. Read More

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September 2021

Neurosteroids and steroid hormones are allosteric modulators of muscarinic receptors.

Neuropharmacology 2021 Sep 20:108798. Epub 2021 Sep 20.

Institute of Physiology Czech Academy of Sciences, Prague, Czech Republic. Electronic address:

The membrane cholesterol was found to bind and modulate the function of several G-protein coupled receptors including muscarinic acetylcholine receptors. We investigated the binding of 20 steroidal compounds including neurosteroids and steroid hormones to muscarinic receptors. Corticosterone, progesterone and some neurosteroids bound to muscarinic receptors with the affinity of 100 nM or greater. Read More

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September 2021

Expanding the target range of base editing in plants without loss of efficiency by blocking RNA-silencing.

Plant Biotechnol J 2021 Sep 23. Epub 2021 Sep 23.

Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.

Bacterial-derived CRISPR/Cas systems are versatile platforms to engineer site-specific gene editing tools. Compared to the canonical Cas9-mediated DNA cleavage systems, which induce a high-proportion of frame-shift mutations, the recently developed base editing (BE) tools allow more precise and predictable base substitutions within a CRISPR/Cas9-defined editing window. Initially, such tools made use of engineered cytosine deaminases or evolved adenine deaminases to catalyze base deamination when fused to a Cas9 nickase (nCas9) (Rees and Liu, 2018). Read More

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September 2021

Bacteria-Targeted MRI Probe-Based Imaging Bacterial Infection and Monitoring Antimicrobial Therapy In Vivo.

Small 2021 Sep 23:e2103627. Epub 2021 Sep 23.

Key Laboratory of Luminescence Analysis and Molecular Sensing, College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715, China.

Despite the significant advances of imaging techniques nowadays, accurate diagnosis of bacterial infections and real-time monitoring the efficacy of antibiotic therapy in vivo still remain huge challenges. Herein, a self-assembling peptide (FFYEGK) and vancomycin (Van) antibiotic molecule co-modified gadolinium (Gd) MRI nanoaggregate probe (GFV) for detecting Staphylococcus aureus (S. aureus) infection in vivo and monitoring the treatment of S. Read More

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September 2021

An ultrapotent pan-β-coronavirus lineage B (β-CoV-B) neutralizing antibody locks the receptor-binding domain in closed conformation by targeting its conserved epitope.

Protein Cell 2021 Sep 23. Epub 2021 Sep 23.

Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences; Shanghai Institute of Infectious Disease and Biosecurity; the Fifth People's Hospital of Shanghai; Shanghai Key Laboratory of Medical Epigenetics, International Co-laboratory of Medical Epigenetics and Metabolism (Ministry of Science and Technology); Institutes of Biomedical Sciences; Biosafety Level 3 Laboratory, Shanghai Medical College, Fudan University, Shanghai, 200032, China.

New threats posed by the emerging circulating variants of SARS-CoV-2 highlight the need to find conserved neutralizing epitopes for therapeutic antibodies and efficient vaccine design. Here, we identified a receptor-binding domain (RBD)-binding antibody, XG014, which potently neutralizes β-coronavirus lineage B (β-CoV-B), including SARS-CoV-2, its circulating variants, SARS-CoV and bat SARSr-CoV WIV1. Interestingly, antibody family members competing with XG014 binding show reduced levels of cross-reactivity and induce antibody-dependent SARS-CoV-2 spike (S) protein-mediated cell-cell fusion, suggesting a unique mode of recognition by XG014. Read More

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September 2021

Diversity of arbuscular mycorrhizal fungi and its chemical drivers across dryland habitats.

Mycorrhiza 2021 Sep 23. Epub 2021 Sep 23.

Environmental Science Centre, Qatar University, PO Box 2713, Doha, Qatar.

Qatar is largely characterized by a hyper-arid climate and low soil fertility which create a stressful soil environment for arbuscular mycorrhizal (AM) fungi. In a study of AM fungal communities and their relationship with soil chemical characteristics, we used a high-throughput sequencing technique to explore AM fungal diversity and community composition in different habitats across Qatar. We identified a total of 79 AM fungal taxa, over 77% of which were species from the Glomeraceae family. Read More

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September 2021

A Novel Miniature CRISPR-Cas13 System for SARS-CoV-2 Diagnostics.

ACS Synth Biol 2021 Sep 21. Epub 2021 Sep 21.

Laboratory for Genome Engineering and Synthetic Biology, Division of Biological Sciences, 4700 King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia.

Rapid, point-of-care (POC) diagnostics are essential to mitigate the impacts of current (and future) epidemics; however, current methods for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) require complicated laboratory tests that are generally conducted off-site and require substantial time. CRISPR-Cas systems have been harnessed to develop sensitive and specific platforms for nucleic acid detection. These detection platforms take advantage of CRISPR enzymes' RNA-guided specificity for RNA and DNA targets and collateral trans activities on single-stranded RNA and DNA reporters. Read More

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September 2021

AddTag, a two-step approach with supporting software package that facilitates CRISPR/Cas-mediated precision genome editing.

G3 (Bethesda) 2021 Sep;11(9)

Department of Molecular and Cell Biology, School of Natural Sciences, University of California, Merced, Merced, CA 95343, USA.

CRISPR/Cas-induced genome editing is a powerful tool for genetic engineering, however, targeting constraints limit which loci are editable with this method. Since the length of a DNA sequence impacts the likelihood it overlaps a unique target site, precision editing of small genomic features with CRISPR/Cas remains an obstacle. We introduce a two-step genome editing strategy that virtually eliminates CRISPR/Cas targeting constraints and facilitates precision genome editing of elements as short as a single base-pair at virtually any locus in any organism that supports CRISPR/Cas-induced genome editing. Read More

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September 2021

In situ remediation of Cr(VI) contaminated groundwater by ZVI-PRB and the corresponding indigenous microbial community responses: a field-scale study.

Sci Total Environ 2021 Sep 11;805:150260. Epub 2021 Sep 11.

Nanjing Kangdi Environmental Protection Technology Co., LTD, Nanjing 21000, China.

The performance of a permeable reactive barrier (PRB) for the in situ remediation of hexavalent chromium [Cr(VI)] contaminated groundwater, and the resulted responses in the indigenous microbial community, were investigated in a field-scale study. The PRB consisted of a mixture of zero-valent iron (ZVI), gravel and sand. The results showed that the PRB segment with 20% active reaction medium (ZVI) was able to successfully reduce Cr(VI) via chemical reduction from 27. Read More

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September 2021

Plants are a natural source of nitrous oxide even in field conditions as explained by N site preference.

Sci Total Environ 2021 Sep 11;805:150262. Epub 2021 Sep 11.

Key Laboratory of Agricultural Water Resources, Hebei Key Laboratory of Soil Ecology, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050021, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Plants are either recognized to produce nitrous oxide (NO) or considered as a medium to transport soil-produced NO. To date, it is not clear whether in their habitat plants conduit NO produced in soil or are a natural source. We aimed to understand role of plants in NO emissions in field conditions. Read More

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September 2021

Site-Fixed Hydroboration of Terminal and Internal Alkenes using BX3/iPr2NEt.

Angew Chem Int Ed Engl 2021 Sep 18. Epub 2021 Sep 18.

Lanzhou Institute of Chemical Physics, CAS, State Key Laboratory for Oxo Synthesis and Selective Oxidation, Tianshui Middle Road, 730000, Lanzhou, CHINA.

An unprecedented and general hydroboration of alkenes with BX3(X = Br, Cl) as the boration reagent in the presence of iPr2NEt is reported. The addition of iPr2NEt not only suppresses alkene polymerization and haloboration side reactions but also provides an "H" source for hydroboration. More importantly, the site-fixed installation of a boryl group at the original position of the internal double-bond is readily achieved as compared with conventional transition-metal-catalyzed hydroboration processes. Read More

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September 2021

Direct, Site-Selective and Redox-Neutral α-C-H Bond Functionalization of Tetrahydrofurans via Quantum Dots Photocatalysis.

Angew Chem Int Ed Engl 2021 Sep 18. Epub 2021 Sep 18.

Technical Institute of Physics and Chemistry, Technical Institute of Physics and Chemistry, CAS, CHINA.

As one of the most ubiquitous bulk reagents available, the intrinsic chemical inertness of tetrahydrofuran (THF) makes direct and site-selective C(sp 3 )-H bond activation difficult, especially under redox neutral condition. Here, we demonstrate that semiconductor quantum dots (QDs) can activate α-C-H bond of THF via forming QDs/THF conjugates. Under visible light irradiation, the resultant alkoxyalkyl radical directly engages in radical cross-coupling with α-amino radical from amino C-H bonds or radical addition with alkene or phenylacetylene, respectively. Read More

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September 2021

A CRISPR/Cas12a-assisted in vitro diagnostic tool for identification and quantification of single CpG methylation sites.

Biosens Bioelectron 2021 Sep 11;194:113624. Epub 2021 Sep 11.

BIOS Lab on a Chip Group, MESA+ Institute for Nanotechnology, Technical Medical Centre, Max Planck Institute for Complex Fluid Dynamics, University of Twente, P.O. box 217 7500 AE Enschede, the Netherlands.

The excellent specificity and selectivity of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/associated nuclease (Cas) is determined by CRISPR RNA's (crRNA's) interchangeable spacer sequence, as well as the position and number of mismatches between target sequence and the crRNA sequence. Some diseases are characterized by epigenetic alterations rather than nucleotide changes, and are therefore unsuitable for CRISPR-assisted sensing methods. Here we demonstrate an in vitro diagnostic tool to discriminate single CpG site methylation in DNA by the use of methylation-sensitive restriction enzymes (MSREs) followed by Cas12a-assisted sensing. Read More

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September 2021

Hydrochemical appraisal and solute acquisitions in Seti River Basin, Central Himalaya, Nepal.

Environ Monit Assess 2021 Sep 17;193(10):656. Epub 2021 Sep 17.

Central Department of Environmental Science, Tribhuvan University, Kathmandu, Nepal.

The chemical characterization and assessment of the water quality in the headwater areas of the Himalaya are necessary for securing the water in the future. This study aims to assess the hydrochemistry and water quality concerning drinking and irrigational uses in the Seti River Basin (SRB), Nepal. A total of 45 water samples were collected in 2016 from the SRB during pre-monsoon, monsoon, and post-monsoon seasons, and pH, EC, TDS, and DO were measured on-site, whereas Ca, Mg, K, Na, Cl, SO, NO, and dissolved Si were analyzed in the laboratory. Read More

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September 2021

A pH-targeted and NIR-responsive NaCl-nanocarrier for photothermal therapy and ion-interference therapy.

Nanomedicine 2021 Sep 13:102460. Epub 2021 Sep 13.

College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China. Electronic address:

Transport ions into cells through nanocarrier to achieve ion-interference therapy provides new inspiration for cancer treatment. In this work, a pH-targeted and NIR-responsive NaCl-nanocarrier is prepared using surfactant Vitamin E-O(EG-Glu) and modified with polydopamine (PDA) and pH-sensitive zwitterionic chitosan (ZWC). The NaCl-nanocarrier is decorated with NHHCO and IR-780 to introduce near-infrared (NIR)-responsive performance and imaging. Read More

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September 2021

Seasonal changes in stable carbon isotopic composition in the bulk aerosol and gas phases at a suburban site in Prague.

Sci Total Environ 2021 Aug 24;803:149767. Epub 2021 Aug 24.

Institute of Chemical Process Fundamentals, Czech Academy of Sciences, Rozvojová 2/135, 165 02, Prague 6, Czech Republic.

Isotope fractionation between the gas and aerosol phases is an important phenomenon for studying atmospheric processes. Here, for the first time, seasonally resolved stable carbon isotope ratio (δC) values are systematically used to study phase interactions in bulk aerosol and gaseous carbonaceous samples. Seasonal variations in the δC of total carbon (TC; δC) and water-soluble organic carbon (WSOC; δC) in fine aerosol particles (PM) as well as in the total carbon of part of the gas phase (TCgas; δC) were studied at a suburban site in Prague, Czech Republic, Central Europe. Read More

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Perpetual atmospheric dry deposition exacerbates the unbalance of dissolved inorganic nitrogen and phosphorus in coastal waters: A case study on a mariculture site in North China.

Mar Pollut Bull 2021 Sep 3;172:112866. Epub 2021 Sep 3.

CAS Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, Shandong 264003, China; University of Chinese Academy of Sciences, Beijing 100049, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, Shandong 266071, China.

The monthly magnitudes of dissolvable nutrients through atmospheric dry deposition (ADD) and their ecological effects to the coastal waters around the Yangma Island, North Yellow Sea, were investigated for one year. The results indicated that anthropogenic activities were the major sources of dissolvable inorganic and organic nitrogen (DIN and DON); dust events were the major sources of inorganic phosphorus (DIP) and silicate (DSi); however, organic phosphorus (DOP) could be originated from marine biological activities. The annual ADD fluxes of DIN, DON, DIP, DOP and DSi were 21. Read More

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September 2021

The pivotal role of cytotoxic NK cells in mediating the therapeutic effect of anti-CD47 therapy in mycosis fungoides.

Cancer Immunol Immunother 2021 Sep 14. Epub 2021 Sep 14.

Cutaneous Lymphoma Program, Department of Dermatology, University of Pittsburgh, 3708 Fifth Avenue, 5th Floor, Suite 500.68, Pittsburgh, PA, 15213, USA.

CD47 is frequently overexpressed on tumor cells and is an attractive therapeutic target. The mechanism by which anti-CD47 immunotherapy eliminates cutaneous lymphoma has not been explored. We utilized CRISPR/Cas-9 CD47 knock-out, depletion of NK cells, and mice genetically deficient in IFN-γ to elucidate the mechanism of anti-CD47 therapy in a murine model of cutaneous T cell lymphoma (CTCL). Read More

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September 2021

The Facile Dissociation of Carbon-Oxygen bonds in CO2 and CO on the Surface of LaCoSiHx Intermetallic Compound.

Angew Chem Int Ed Engl 2021 Sep 14. Epub 2021 Sep 14.

Institute of Coal Chemistry, Chinese Academy of Sciences, State Key of Laboratory for Coal Coversion, 27 Taoyuan South Road, 030001, Taiyuan, CHINA.

In catalysis science, the electronic structure of the active site determines the structure-activity relationship of the catalyst to a large extent. Therefore, modulating the electronic structure has become a main route for the rational design of metal-based catalyst materials. In this work, we prepared a LaCoSiH x material that has more electronegativity and a lower workfunction than traditional supported Co-based catalysts. Read More

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September 2021

Delivery of local anaesthetics by a self-assembled supramolecular system mimicking their interactions with a sodium channel.

Nat Biomed Eng 2021 Sep 13. Epub 2021 Sep 13.

Laboratory for Biomaterials and Drug Delivery, Department of Anaesthesiology, Division of Critical Care Medicine, Children's Hospital Boston, Harvard Medical School, Boston, MA, USA.

Site-1 sodium channel blockers (S1SCBs) act as potent local anaesthetics, but they can cause severe systemic toxicity. Delivery systems can be used to reduce the toxicity, but the hydrophilicity of S1SCBs makes their encapsulation challenging. Here, we report a self-assembling delivery system for S1SCBs whose design is inspired by the specific interactions of S1SCBs with two peptide sequences on the sodium channel. Read More

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September 2021

CRISPR mediated genome editing, a tool to dissect RNA modification processes.

Methods Enzymol 2021 8;658:435-452. Epub 2021 Jul 8.

Department of Biology, Ball State University, Muncie, IN, United States. Electronic address:

Though over 100 distinct RNA modifications have been identified, the roles for many of these modifications in vivo remain unknown. Genome editing is one tool investigators are using to better understand the roles these modifications play and the consequences of their absence. In this chapter, we describe how CRISPR mediated genome editing can be used to interrogate the process of RNA modification in C. Read More

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September 2021

Effects of elevated CO concentration and nitrogen addition on foliar phosphorus fractions of Mikania micranatha and Chromolaena odorata under low phosphorus availability.

Physiol Plant 2021 Sep 13. Epub 2021 Sep 13.

CAS Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.

Invasive plants rapidly spread in habitats with low soil phosphorus (P) availability and have triggered a sharp decline in the diversity of native species. However, no studies have explored how widespread invasive species acclimate to low soil P availability via changing foliar P fractions, especially under elevated atmospheric CO concentrations ([CO ]) and nitrogen (N) deposition. Here, an open-top chamber experiment was conducted to explore the effect of nutrient addition and elevated [CO ] on leaf traits and foliar functional P fractions (i. Read More

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September 2021

G-Quadruplex-Probing CRISPR-Cas12 Assay for Label-Free Analysis of Foodborne Pathogens and Their Colonization .

ACS Sens 2021 Sep 13;6(9):3295-3302. Epub 2021 Sep 13.

College of Biomass Science and Engineering, Healthy Food Evaluation Research Center, Sichuan University, Chengdu 610065, China.

Foodborne pathogen infection is a key issue of food safety. Herein, we developed a label-free assay for () detection based on the G-quadruplex-probing CRISPR-Cas12 system (termed G-CRISPR-Cas), allowing highly sensitive detection of and investigation of their colonization in chickens. The introduction of the G-quadruplex probe serving as the substrate of Cas 12a realized a label-free analysis for foodborne pathogens. Read More

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September 2021

Observation of ambient NO radicals by LP-DOAS at a rural site in North China Plain.

Sci Total Environ 2021 Aug 27;804:149680. Epub 2021 Aug 27.

Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China; University of Chinese Academy of Sciences, Beijing 100049, China.

NO radicals can clean the atmospheric primary contaminants during the night. However, it can also effect the formation of secondary organic aerosol (SOA) and nitrate, which may worsen air quality. We report field observations of NO radicals with a home-made long path differential optical absorption spectroscopy (LP-DOAS) at a rural site in the polluted North China Plain in the summer of 2014. Read More

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The BODIPY-based chemosensor for the fluorometric determination of organochlorine pesticide dicofol.

Food Chem 2021 Sep 2;370:131033. Epub 2021 Sep 2.

Department of Instrumentation and Analytical Chemistry, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, PR China. Electronic address:

Dicofol is an organochlorine pesticide, which is widely used in fruits, tea and other crops, and is moderately toxic to humans. Therefore, the monitoring of organochlorine pesticide-dicofol is critical for food safety. In this work, a fluorometric chemosensor based on mercaptoethanol and boron dipyrromethene (BODIPY) was first constructed to detect the dicofol. Read More

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September 2021

Insight into the role of varied acid-base sites on fast pyrolysis kinetics and mechanism of cellulose.

Waste Manag 2021 Sep 8;135:140-149. Epub 2021 Sep 8.

School of Energy Science and Engineering, University of Science and Technology of China, Hefei 230026, PR China; Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences (CAS), Guangzhou 510640, PR China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), 511458, PR China; CAS Key Laboratory of Renewable Energy, Guangzhou 510640, PR China; Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, PR China.

In the present research, a series of metal oxides were employed to investigate the role of varied acid-base site on kinetics and mechanism for catalytic fast pyrolysis of cellulose using TG-MS and Py-GC/MS. The results showed that the modulation of acidity-to-basicity value via altering metal oxides constituents significantly affected the transformation pathway for cellulose pyrolysis. Higher acidity-to-basicity ratios accelerated the proceeding of deoxygenation process, wherein 39. Read More

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September 2021

Novel van der Waals Deep-UV Nonlinear Optical Materials.

Chemistry 2021 Sep 10. Epub 2021 Sep 10.

Techincal Institute of Physics and Chemistry, CAS, Beijng Center for Crystal R&D, 29, Zhong Guan Cun East Road, 100190, Beijing, CHINA.

Van der Waals (vdW) deep-UV (DUV) nonlinear optical (NLO) crystal is an important material system recently developed. Herein, we reviewed its concept and original intention, and then summarized the discovery process of related materials, including the role of A-site cations and the resulting two-/one-dimensional vdW DUV NLO systems. Finally, we evaluated the practical DUV NLO performance and prospected the opportunities and challenges. Read More

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September 2021

[Preparation of [email protected] magnetic solid-phase extraction material and its application to the detection of pesticide residues in tea].

Se Pu 2021 Oct;39(10):1111-1117

CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Tea is one of the most popular beverages worldwide, and its quality is often affected by the excessive pesticide residues during production. During the detection of pesticide residues in tea by chromatography-mass spectrometry and other methods, a strong matrix effect attributed to tea polyphenols and pigments is observed, which seriously impacts the analysis results. In this study, FeO magnetic nanoparticles and boric acid-functionalized metal organic framework (BA-MOF) materials were combined to prepare a highly efficient adsorbent [email protected] for capturing tea polyphenols and pigments. Read More

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October 2021

[Advances in chromatography in the study of drug-plasma protein interactions].

Se Pu 2021 Oct;39(10):1077-1085

CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

After entering human blood circulation, small-molecule drugs interact extensively with various plasma proteins, such as human serum albumin and α-acid glycoprotein. These interactions profoundly affect the distribution of drugs in vivo and the binding of drugs to targets, thus affecting the efficacy of drugs. In-depth investigation of drug-plasma protein interactions is of great significance for the optimization of drug properties, the development of new drugs, risk assessment, and combination therapy of drugs. Read More

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October 2021

[Recent advances in glycopeptide enrichment and mass spectrometry data interpretation approaches for glycoproteomics analyses].

Se Pu 2021 Oct;39(10):1045-1054

CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.

Protein glycosylation is one of the most important post-translational modifications (PTMs). The glycosylation is crucial in a variety of physiological and pathological processes that include protein stability, intracellular and intercellular signal transduction, hormone activation or inactivation, and immune regulation. Protein glycosylation is generated by complex biosynthetic pathways comprising hundreds of glycosyltransferases, glycosidases, transcriptional factors, transporters, and protein backbones. Read More

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October 2021