62,979 results match your criteria single strains


Advancement of chimeric hybrid drugs to cure malaria infection: An overview with special emphasis on endoperoxide pharmacophores.

Eur J Med Chem 2021 Apr 4;219:113408. Epub 2021 Apr 4.

Chemical Biology Laboratory, Department of Chemistry, University of Delhi, Delhi 110007, India. Electronic address:

Emergence and spread of Plasmodium falciparum resistant to artemisinin-based combination therapy has led to a situation of haste in the scientific and pharmaceutical communities. Sincere efforts are redirected towards finding alternative chemotherapeutic agents that are capable of combating multidrug-resistant parasite strains. Extensive research yielded the concept of "Chimeric Bitherapy (CB)" which involves the linking of two molecules with individual pharmacological activity and exhibit dual mode of action into a single hybrid molecule. Read More

View Article and Full-Text PDF

De novo transcriptome assembly of the green alga Ankistrodesmus falcatus.

PLoS One 2021 14;16(5):e0251668. Epub 2021 May 14.

Department of Biological Sciences, University of South Carolina, Columbia, SC, United States of America.

Ankistrodesmus falcatus is a globally distributed freshwater chlorophyte that is a candidate for biofuel production, is used to study the effects of toxins on aquatic communities, and is used as food in zooplankton research. Each of these research fields is transitioning to genomic tools. We created a reference transcriptome for of A. Read More

View Article and Full-Text PDF

Exceptional Deformability of Wurtzite Zinc Oxide Nanowires with Growth Axial Stacking Faults.

Nano Lett 2021 May 14. Epub 2021 May 14.

School of Mechanical, Medical, and Process Engineering, Queensland University of Technology (QUT), Brisbane, Queensland 4001, Australia.

To ensure reliability and facilitate the strain engineering of zinc oxide (ZnO) nanowires (NWs), it is significant to understand their flexibility thoroughly. In this study, single-crystalline ZnO NWs with rich axial pyramidal I () and prismatic stacking faults (SFs) are synthesized by a metal oxidation method. Bending properties of the as-synthesized ZnO NWs are investigated at the atomic scale using an high-resolution transmission electron microscopy (HRTEM) technique. Read More

View Article and Full-Text PDF

Characterization of Gene Expression Signatures for the Identification of Cellular Heterogeneity in the Developing Mammary Gland.

J Mammary Gland Biol Neoplasia 2021 May 14. Epub 2021 May 14.

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 11724, US.

The developing mammary gland depends on several transcription-dependent networks to define cellular identities and differentiation trajectories. Recent technological advancements that allow for single-cell profiling of gene expression have provided an initial picture into the epithelial cellular heterogeneity across the diverse stages of gland maturation. Still, a deeper dive into expanded molecular signatures would improve our understanding of the diversity of mammary epithelial and non-epithelial cellular populations across different tissue developmental stages, mouse strains and mammalian species. Read More

View Article and Full-Text PDF

Adhesive Functions or Pseudogenization of Type Va Autotransporters in Species.

Front Cell Infect Microbiol 2021 27;11:607610. Epub 2021 Apr 27.

Fundación Instituto Leloir (FIL), IIBBA (CONICET-FIL), Buenos Aires, Argentina.

Adhesion to host cells is a key step for successful infection of many bacterial pathogens and may define tropism to different host tissues. To do so, bacteria display adhesins on their surfaces. is an intracellular pathogen capable of proliferating in a wide variety of cell types. Read More

View Article and Full-Text PDF

Four Complete Genome Sequences for sp. Strains Isolated from an Endemic Australian Legume Reveal Structural Variation.

Microbiol Resour Announc 2021 May 13;10(19). Epub 2021 May 13.

Research School of Biology, Australian National University, Canberra, Australian Capital Territory, Australia

sp. strains were isolated from root nodules of the Australian legume, (Fabaceae). Here, we report the complete genome sequences of four strains using a hybrid long- and short-read assembly approach. Read More

View Article and Full-Text PDF

Gametic specialization of centromeric histone paralogs in .

Life Sci Alliance 2021 Jul 13;4(7). Epub 2021 May 13.

Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA, USA

In most eukaryotes, centromeric histone (CenH3) proteins mediate mitosis and meiosis and ensure epigenetic inheritance of centromere identity. We hypothesized that disparate chromatin environments in soma versus germline might impose divergent functional requirements on single CenH3 genes, which could be ameliorated by gene duplications and subsequent specialization. Here, we analyzed the cytological localization of two recently identified CenH3 paralogs, Cid1 and Cid5, in using specific antibodies and epitope-tagged transgenic strains. Read More

View Article and Full-Text PDF

Designing potential siRNA molecule for the nucleocapsid(N) gene silencing of different SARS-CoV-2 strains of Bangladesh: Computational approach.

Comput Biol Chem 2021 May 6;92:107486. Epub 2021 May 6.

Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, 1000, Bangladesh. Electronic address:

SARS-CoV-2 is a single-stranded RNA (+) virus first identified in China and then became an ongoing global outbreak. In most cases, it is fatal in humans due to respiratory malfunction. Extensive researches are going to find an effective therapeutic technique for the treatment of SARS-CoV-2 infected individuals. Read More

View Article and Full-Text PDF

The PAR promoter expression system: modified lac promoters for controlled recombinant protein production in Escherichia coli.

N Biotechnol 2021 May 10. Epub 2021 May 10.

Institute of Microbiology and Infection and School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK; College of Health and Life Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, UK. Electronic address:

Many commonly used bacterial promoters employed for recombinant protein production (RPP) in Escherichia coli are capable of high-level protein expression. However, such promoter systems are often too strong, being ill suited for expressing proteins that are difficult to fold, targeted to the membrane or secreted out of the cytoplasm. To circumvent this problem, a suite of bacterial promoters has been constructed with a range of different promoter strengths, assigning them specific "promoter activity ratings" (PARs). Read More

View Article and Full-Text PDF

A Tail Fiber Engineering Platform for Improved Bacterial Transduction-Based Diagnostic Reagents.

ACS Synth Biol 2021 May 13. Epub 2021 May 13.

Roche Molecular Systems, Santa Clara, California 95050, United States.

Bacterial transduction particles were critical to early advances in molecular biology and are currently experiencing a resurgence in interest within the diagnostic and therapeutic fields. The difficulty of developing a robust and specific transduction reagent capable of delivering a genetic payload to the diversity of strains constituting a given bacterial species or genus is a major impediment to their expanded utility as commercial products. While recent advances in engineering the reactivity of these reagents have made them more attractive for product development, considerable improvements are still needed. Read More

View Article and Full-Text PDF

Efficient delivery of oncolytic enterovirus by carrier cell line NK-92.

Mol Ther Oncolytics 2021 Jun 1;21:110-118. Epub 2021 Apr 1.

Department of Peptide and Protein Technologies, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow 117997, Russia.

Many members of the enterovirus family are considered as promising oncolytic agents; however, their systemic administration is largely inefficient due to the rapid neutralization of the virus in the circulation and the barrier functions of the endothelium. We aimed to evaluate natural killer cells as carriers for the delivery of oncolytic enteroviruses, which would combine the effects of cell immunotherapy with virotherapy. We tested four strains of nonpathogenic enteroviruses against the glioblastoma cell line panel and evaluated the produced infectious titers. Read More

View Article and Full-Text PDF

Multispacer Sequence Typing for Genotyping.

Front Vet Sci 2021 26;8:666283. Epub 2021 Apr 26.

Universidade de São Paulo, São Paulo, São Paulo, Brazil.

The molecular typing of , which causes bovine tuberculosis, can be accomplished by combining different polymorphic markers, contributing to its epidemiological investigation. Multispacer sequence typing (MST) is a sequencing-based method that employs intergenic regions susceptible to higher mutation rates given the low selection pressure. It has been applied to , but not to . Read More

View Article and Full-Text PDF

Genome Investigation of Urinary Strains and Their Relationship to Isolates of the Vaginal Microbiota.

mSphere 2021 May 12;6(3). Epub 2021 May 12.

Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, Illinois, USA.

is a frequent member of the urogenital microbiota. Given the association between and bacterial vaginosis (BV), significant efforts have been focused on characterizing this species in the vaginal microbiota. However, also is a frequent member of the urinary microbiota. Read More

View Article and Full-Text PDF

Streptococcus suis Encodes Multiple Allelic Variants of a Phase-Variable Type III DNA Methyltransferase, ModS, That Control Distinct Phasevarions.

mSphere 2021 May 12;6(3). Epub 2021 May 12.

Institute for Glycomics, Griffith University, Gold Coast, Queensland, Australia

is a significant cause of bacterial meningitis in humans, particularly in Southeast Asia, and is a leading cause of respiratory and invasive disease in pigs. Phase-variable DNA methyltransferases, associated with restriction-modification (R-M) systems, are a source of epigenetic gene regulation, controlling the expression of multiple genes. These systems are known as phasevarions (phase-variable regulons) and have been characterized in many host-adapted bacterial pathogens. Read More

View Article and Full-Text PDF

Development and validation of a core genome multilocus sequence typing scheme to facilitate molecular surveillance.

J Clin Microbiol 2021 May 12. Epub 2021 May 12.

Institute of Hygiene, Microbiology and Environmental Medicine, Medical University Graz, Graz, Austria

causes the severe disease melioidosis. Whole genome-sequencing (WGS) based typing methods currently offer the highest resolution for molecular investigations of this genetically diverse pathogen. Still, its routine application in diagnostic laboratories is limited by the need for high computing power, bioinformatic skills and variable bioinformatic approaches, the latter affecting the results. Read More

View Article and Full-Text PDF

Interplay between H1N1 influenza a virus infection, extracellular and intracellular respiratory tract pH, and host responses in a mouse model.

PLoS One 2021 12;16(5):e0251473. Epub 2021 May 12.

Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, United States of America.

During influenza A virus (IAV) entry, the hemagglutinin (HA) protein is triggered by endosomal low pH to undergo irreversible structural changes that mediate membrane fusion. HA proteins from different isolates vary in the pH at which they become activated in endosomes or become irreversible inactivated if exposed to extracellular acid. Little is known about extracellular pH in the upper respiratory tracts of mammals, how pH may shift during IAV infection, and its impact on replication of viruses that vary in HA activation pH. Read More

View Article and Full-Text PDF

Discovery of human ACE2 variants with altered recognition by the SARS-CoV-2 spike protein.

PLoS One 2021 12;16(5):e0251585. Epub 2021 May 12.

Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, United States of America.

Understanding how human ACE2 genetic variants differ in their recognition by SARS-CoV-2 can facilitate the leveraging of ACE2 as an axis for treating and preventing COVID-19. In this work, we experimentally interrogate thousands of ACE2 mutants to identify over one hundred human single-nucleotide variants (SNVs) that are likely to have altered recognition by the virus, and make the complementary discovery that ACE2 residues distant from the spike interface influence the ACE2-spike interaction. These findings illuminate new links between ACE2 sequence and spike recognition, and could find substantial utility in further fundamental research that augments epidemiological analyses and clinical trial design in the contexts of both existing strains of SARS-CoV-2 and novel variants that may arise in the future. Read More

View Article and Full-Text PDF

Tryptophan Operon Diversity Reveals Evolutionary Trends among Geographically Disparate Chlamydia trachomatis Ocular and Urogenital Strains Affecting Tryptophan Repressor and Synthase Function.

mBio 2021 May 11;12(3). Epub 2021 May 11.

Department of Pediatrics, University of California San Francisco, Oakland, California, USA

The obligate intracellular pathogen () is the leading cause of bacterial sexually transmitted infections and blindness globally. To date, urogenital strains are considered tryptophan prototrophs, utilizing indole for tryptophan synthesis within a closed-conformation tetramer comprised of two α (TrpA)- and two β (TrpB)-subunits. In contrast, ocular strains are auxotrophs due to mutations in TrpA, relying on host tryptophan pools for survival. Read More

View Article and Full-Text PDF

Evolvement of taste sensitivity and taste buds in chickens during selective breeding.

Poult Sci 2021 Mar 11;100(6):101113. Epub 2021 Mar 11.

Regenerative Bioscience Center, University of Georgia, Athens, GA, USA; Department of Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia, Athens, GA, USA. Electronic address:

Chickens have been reported to have a low taste bud count and thus low taste acuity. However, more recent studies indicate that the earlier reported count of chicken taste buds may have been significantly underestimated. To answer the question of whether the taste sensing system in broiler chickens evolved during the breeding selection over the past decades, we compared the taste sensitivity to bitter and taste buds between a meat-type control strain - the 1955 Athens Canadian Random Bred (ACRB), and a modern high-yielding broiler strain - the 2012 Cobb 500. Read More

View Article and Full-Text PDF

Antimicrobial activities of Bacillus velezensis strains isolated from stingless bee products against methicillin-resistant Staphylococcus aureus.

PLoS One 2021 11;16(5):e0251514. Epub 2021 May 11.

Enzyme and Microbial Technology Research Center, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.

Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) have reached epidemic proportions globally. Therefore, there is an urgent need for a continuous supply of antibiotics to combat the problem. In this study, bacteria initially identified as species belonging to the Bacillus amyloliquefaciens operational group were re-identified based on the housekeeping gene, gyrB. Read More

View Article and Full-Text PDF

Tissue-specific regulation of epidermal contraction during C. elegans embryonic morphogenesis.

G3 (Bethesda) 2021 May 11. Epub 2021 May 11.

Department of Biochemistry and Molecular Biology, Alberta Children's Hospital Research Institute, University of Calgary, Calgary, Alberta T2N 4N1, Canada.

Actin and myosin mediate the epidermal cell contractions that elongate the Caenorhabditis elegans embryo from an ovoid to a tubular-shaped worm. Contraction occurs mainly in the lateral epidermal cells, while the dorsoventral epidermis plays a more passive role. Two parallel pathways trigger actinomyosin contraction, one mediated by LET-502/Rho kinase and the other by PAK-1/p21 activated kinase. Read More

View Article and Full-Text PDF

Gut microbiota modulation and anti-inflammatory properties of mixed lactobacilli in dextran sodium sulfate-induced colitis in mice.

Food Funct 2021 May 11. Epub 2021 May 11.

Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China. and Food College, Northeast Agricultural University, Harbin 150030, China.

Correlations between gut microbiota activities and inflammatory bowel disease (IBD) treatment are gaining research interest. In our previous study, Lactobacillus acidophilus KLDS 1.0901, Lactobacillus helveticus KLDS 1. Read More

View Article and Full-Text PDF

Population genomics study of Vibrio alginolyticus.

Yi Chuan 2021 Apr;43(4):350-361

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.

Vibrio alginolyticus is a Gram-negative bacillus that causes vibriosis to human and aquatic products, including fish, shrimp and shellfish. It poses a threat to public health and causes enormous economic losses to the aquaculture industry. However, research on genetic diversity and pathogenicity-related genetic elements based on whole genome is still lacking. Read More

View Article and Full-Text PDF

Safe Polycationic Dendrimers as Potent Oral In Vivo Inhibitors of : A New Therapy to Take Down Tuberculosis.

Biomacromolecules 2021 May 10. Epub 2021 May 10.

Laboratoire de Chimie de Coordination du CNRS, 205 Route de Narbonne, BP 44099, 31077 Toulouse Cedex 4, France.

The long-term treatment of tuberculosis (TB) sometimes leads to nonadherence to treatment, resulting in multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis. Inadequate bioavailability of the drug is the main factor for therapeutic failure, which leads to the development of drug-resistant cases. Therefore, there is an urgent need to design and develop novel antimycobacterial agents minimizing the period of treatment and reducing the propagation of resistance at the same time. Read More

View Article and Full-Text PDF

X-Ray Photoelectron Spectroscopy on Microbial Cell Surfaces: A Forgotten Method for the Characterization of Microorganisms Encapsulated With Surface-Engineered Shells.

Front Chem 2021 22;9:666159. Epub 2021 Apr 22.

University of Groningen and University Medical Center of Groningen, Department of Biomedical Engineering, Groningen, Netherlands.

Encapsulation of single microbial cells by surface-engineered shells has great potential for the protection of yeasts and bacteria against harsh environmental conditions, such as elevated temperatures, UV light, extreme pH values, and antimicrobials. Encapsulation with functionalized shells can also alter the surface characteristics of cells in a way that can make them more suitable to perform their function in complex environments, including bio-reactors, bio-fuel production, biosensors, and the human body. Surface-engineered shells bear as an advantage above genetically-engineered microorganisms that the protection and functionalization added are temporary and disappear upon microbial growth, ultimately breaking a shell. Read More

View Article and Full-Text PDF

Induction of spontaneous phenotype conversion in Ralstonia solanacearum by addition of iron compounds in liquid medium.

J Microbiol Methods 2021 May 6:106233. Epub 2021 May 6.

Graduate School of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, Tsukide 3-1-100, Higashi-ku, Kumamoto 862-8502, Japan.

Ralstonia solanacearum is a soil-borne pathogen that causes bacterial wilt in plants. The wild-type strain of R. solanacearum undergoes spontaneous phenotype conversion (PC), from a fluidal to non-fluidal colony morphology. Read More

View Article and Full-Text PDF

Synthesis, antimicrobial, anti-cancer and in silico studies of new urea derivatives.

Bioorg Chem 2021 Apr 29;112:104953. Epub 2021 Apr 29.

Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Sadat City, Menoufia, Egypt.

The reaction of an alkyl or aryl isocyanates with some primary amines in acetonitrile at room temperature afforded the corresponding alkyl- and aryl-urea derivatives. All the prepared urea compounds have been elucidated by FTIR, NMR, and elemental analysis. The compounds 1 and 3 were confirmed by single-crystal X-ray diffraction. Read More

View Article and Full-Text PDF

Genomic variation of an endosymbiotic dinoflagellate (Symbiodinium 'fitti') among closely related coral hosts.

Mol Ecol 2021 May 8. Epub 2021 May 8.

Department of Biology, The Pennsylvania State University, University Park, PA, 16801, USA.

Mutualisms where hosts are coupled metabolically to their symbionts often exhibit high partner fidelity. Most reef-building coral species form obligate symbioses with a specific species of photosymbionts, dinoflagellates in the family Symbiodiniaceae, despite needing to acquire symbionts early in their development from environmental sources. Three Caribbean acroporids (Acropora palmata, A. Read More

View Article and Full-Text PDF

Phylogenetics of Bacillus anthracis isolates from Russia and bordering countries.

Infect Genet Evol 2021 May 5;92:104890. Epub 2021 May 5.

Federal Government Health Institution «Stavropol Plague Control Research Institute» of the Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare, 13-15 Sovetskaya Str, 355035 Stavropol, Russian Federation.

Anthrax is a concern for public health and veterinary medicine in Russia. The available phylogenetic data on isolates from Russia and neighboring CIS countries are clearly not enough to gain a better understanding of their position in the global phylogenetic population structure of this pathogen. In this study, we analyzed the genomes of 66 Bacillus anthracis strains, which were isolated between 1935 and 2019 from different sources in Russia, as well as in Ukraine, Azerbaijan, Georgia, Armenia and Moldova. Read More

View Article and Full-Text PDF

Giant Piezoresistive Effect of CdS@C Hybrid Nanobelts for Volatile Real-Time Sensor and Erasable Nonvolatile Memory to Stress.

ACS Appl Mater Interfaces 2021 May 7. Epub 2021 May 7.

Nanoscale Science and Technology Laboratory, Institute for Advanced Study, Nanchang University, Jiangxi 330031, P. R. China.

Here, CdS@C nanohybrid composites, where CdS quantum dots (QDs) are uniformly embedded in carbon micro-/nanobelt matrixes, are synthesized via a combustion synthesis followed by a postvulcanization. In the nanohybrids, trap centers are effectively created by the introduction of QDs and moreover their barrier height and filling level can be effectively modulated through a coupling of externally loaded strain and bias. Thus, a single CdS@C micro-/nanobelt-based two-terminal device can exhibit an ultrahigh real-time response to compressive and tensile strains with a tremendous gauge factor of above 10, high sensitivity, and fast response and recovery. Read More

View Article and Full-Text PDF