N Biotechnol 2021 May 10. Epub 2021 May 10.
Institute of Microbiology and Infection and School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK; College of Health and Life Sciences, Aston University, Aston Triangle, Birmingham, B4 7ET, UK. Electronic address:
Many commonly used bacterial promoters employed for recombinant protein production (RPP) in Escherichia coli are capable of high-level protein expression. However, such promoter systems are often too strong, being ill suited for expressing proteins that are difficult to fold, targeted to the membrane or secreted out of the cytoplasm. To circumvent this problem, a suite of bacterial promoters has been constructed with a range of different promoter strengths, assigning them specific "promoter activity ratings" (PARs). Read More