46,066 results match your criteria single differential

Cognitive processing of sexual cues in asexual individuals and heterosexual women with desire/arousal difficulties.

PLoS One 2021 12;16(5):e0251074. Epub 2021 May 12.

Department of Obstetrics and Gynaecology, University of British Columbia, Vancouver, Canada.

Asexuality is defined as a unique sexual orientation characterized by a lack of sexual attraction to others. This has been challenged, with some experts positing that it is better explained as a sexual dysfunction. Sexual Interest/Arousal Disorder (SIAD) is characterized by absent/reduced sexual interest/arousal paired with personal distress, with two subtypes: acquired and lifelong. Read More

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ProteoMill: Efficient network-based functional analysis portal for proteomics data.

Bioinformatics 2021 May 12. Epub 2021 May 12.

Faculty of Medicine, Department of Clinical Sciences Lund, Rheumatology and Molecular Skeletal Biology, Lund University, Lund, Sweden.

Summary: Functional analysis has become a common approach to incorporate biological knowledge into the analysis of omics data, and to explore molecular events that govern a disease state. It is though only one step in a wider analytical pipeline that typically requires use of multiple individual analysis software. There is currently a need for a well-integrated omics analysis tool that performs all the steps. Read More

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Impact of DNA Adduct Size, Number, and Relative Position on the Toxicity of Aromatic Amines: A Molecular Dynamics Case Study of dG- and dG-Containing DNA Duplexes.

J Chem Inf Model 2021 May 12. Epub 2021 May 12.

Department of Chemistry and Biochemistry, University of Lethbridge, 4401 University Drive West, Lethbridge, AB T1K 3M4, Canada.

Human exposure to aromatic amines (AAs) can result in carcinogenic DNA adducts. To complement previous work geared toward understanding the mutagenicity of AA-derived adducts, which has almost exclusively studied (monoadducted) DNA containing a single lesion, the present work provides the first in-depth comparison of the structure of monoadducted and diadducted DNA duplexes. Specifically, molecular dynamics (MD) simulations were initially performed on DNA containing the nonmutagenic single-ringed -(deoxyguanosin-8-yl)-aniline (dG) or the mutagenic four-ringed -(deoxyguanosin-8-yl)-1-aminopyrene (dG) lesion at G, G, or G in the AA deletion hotspot (5'-GGCGCC) in the or glycosidic orientation (B/S duplex conformation). Read More

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Novel and known miRNAs in zebu (Tharparkar) and crossbred (Karan-Fries) cattle under heat stress.

Funct Integr Genomics 2021 May 11. Epub 2021 May 11.

Animal Biochemistry Division, ICAR-National Dairy Research Institute, Karnal, 132001, Haryana, India.

MicroRNAs (miRNAs) are small single-stranded non-coding RNAs that act as the master regulator of animal growth and development. RNA-RNA interaction is an important mechanism of gene regulation during biotic and abiotic stress. Heat stress (HS) is one of the most important abiotic factors which affect the growth, milk yield and reproductive health of the dairy animals. Read More

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What are the applications of single-cell RNA sequencing in cancer research: a systematic review.

J Exp Clin Cancer Res 2021 May 11;40(1):163. Epub 2021 May 11.

NHC Key Laboratory of Carcinogenesis and Hunan Key Laboratory of Cancer Metabolism, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, China.

Single-cell RNA sequencing (scRNA-seq) is a tool for studying gene expression at the single-cell level that has been widely used due to its unprecedented high resolution. In the present review, we outline the preparation process and sequencing platforms for the scRNA-seq analysis of solid tumor specimens and discuss the main steps and methods used during data analysis, including quality control, batch-effect correction, normalization, cell cycle phase assignment, clustering, cell trajectory and pseudo-time reconstruction, differential expression analysis and gene set enrichment analysis, as well as gene regulatory network inference. Traditional bulk RNA sequencing does not address the heterogeneity within and between tumors, and since the development of the first scRNA-seq technique, this approach has been widely used in cancer research to better understand cancer cell biology and pathogenetic mechanisms. Read More

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WDNE: an integrative graphical model for inferring differential networks from multi-platform gene expression data with missing values.

Brief Bioinform 2021 May 11. Epub 2021 May 11.

Department of Electrical Engineering, City University of Hong Kong, Hong Kong, 999077, China.

The mechanisms controlling biological process, such as the development of disease or cell differentiation, can be investigated by examining changes in the networks of gene dependencies between states in the process. High-throughput experimental methods, like microarray and RNA sequencing, have been widely used to gather gene expression data, which paves the way to infer gene dependencies based on computational methods. However, most differential network analysis methods are designed to deal with fully observed data, but missing values, such as the dropout events in single-cell RNA-sequencing data, are frequent. Read More

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Perception of usefulness of laboratory tests ordering by internal medicine residents in ambulatory setting: A single-center prospective cohort study.

PLoS One 2021 11;16(5):e0250769. Epub 2021 May 11.

Central Laboratory Division, Hospital das Clínicas HCFMUSP, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil.

The demand for high value health care uncovered a steady trend in laboratory tests ordering and inappropriate testing practices. Residents' training in laboratory ordering practice provides an opportunity for quality improvement. We collected information on demographics, the main reason for the appointment, preexisting medical conditions and presence of co-morbidities from first-visit patients to the internal medicine outpatient service of our university general hospital. Read More

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Architecture-Dependent Mechano-Adaptation in Single Vascular Smooth Muscle Cells.

J Biomech Eng 2021 May 11. Epub 2021 May 11.

University of Minnesota - Department of Biomedical Engineering, Nils Hasselmo Hall, Room 7-105, 312 Church St. SE, Minneapolis, MN 55455.

Arteries grow and remodel following mechanical perturbation. Vascular smooth muscle cells (VSMCs) within the artery undergo hyperplasia, hypertrophy, or change their contractility following sustained changes in loading. Experimental evidence suggests that VSMCs grow and remodel to maintain a constant transmural stress, or "target" stress. Read More

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Radiologic findings that aid in the reduction of misdiagnoses of Langerhans cell histiocytosis of the bone: a retrospective study.

World J Surg Oncol 2021 May 10;19(1):146. Epub 2021 May 10.

Department of Radiology, The Affiliated Hospital of Qingdao University, Qingdao, 266003, China.

Background: This study aimed to identify the characteristic radiological signs for the diagnosis of Langerhans cell histiocytosis (LCH) of the bone.

Methods: We retrospectively studied 82 cases of LCH with bone lesions confirmed by pathology. Clinical and radiological features of the patients were analyzed. Read More

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Censcyt: censored covariates in differential abundance analysis in cytometry.

BMC Bioinformatics 2021 May 10;22(1):235. Epub 2021 May 10.

Department of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.

Background: Innovations in single cell technologies have lead to a flurry of datasets and computational tools to process and interpret them, including analyses of cell composition changes and transition in cell states. The diffcyt workflow for differential discovery in cytometry data consist of several steps, including preprocessing, cell population identification and differential testing for an association with a binary or continuous covariate. However, the commonly measured quantity of survival time in clinical studies often results in a censored covariate where classical differential testing is inapplicable. Read More

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Differential gene expression analysis for multi-subject single cell RNA sequencing studies with aggregateBioVar.

Bioinformatics 2021 May 10. Epub 2021 May 10.

Department of Internal Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, USA.

Motivation: Single cell RNA sequencing (scRNA-seq) studies provide more granular biological information than bulk RNA sequencing, but bulk RNA sequencing has remained popular due to relatively lower costs per sample, which has allowed investigators to process more biological replicates and design more powerful studies. As scRNA-seq costs have decreased, collecting data from more than one biological replicate has become more feasible, but careful modeling of different layers of biological variation remains challenging for many users. Here, we propose a statistical model for scRNA-seq gene counts, describe a simple method for estimating model parameters, and show that failing to account for additional biological variation in scRNA-seq studies can inflate false discovery rates of statistical tests. Read More

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Synthesis and characterization of promising insensitive energetic salts based on 3-amino-5-hydrazinopyrazole.

Dalton Trans 2021 May 10. Epub 2021 May 10.

State Key Laboratory of Explosion Science and Technology, Beijing Institute of Technology, Beijing 100081, P. R. China.

The development of green energetic materials is based on environmental friendliness, safety and performance improvement. It is of great significance to design and synthesize new nitrogen rich salts for a new generation of green energetic materials. In the present work, a series of 3-amino-5-hydrazinopyrazole energetic salts comprising energetic anions were synthesized and were characterized using elemental analysis, IR spectroscopy and differential scanning calorimetry (DSC). Read More

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Adolescents Adjudicated for Sexual Offending: Differences Between Sexual Reoffenders and Sexual Non-Reoffenders.

J Interpers Violence 2021 May 10:8862605211015209. Epub 2021 May 10.

Universidad Nacional de Educación a Distancia (UNED), Madrid, Spain.

Although a very small group of adolescents adjudicated for sexual offending (ASO) will persist into adulthood, the use of official records of sexual recidivism after long-term follow-up periods underrates the repetition of sexually coercive behaviors during adolescence. Additionally, limited research has considered sexual reoffending as a criterion to classify this heterogeneous population. The aims of this study were to determine the prevalence of ASO who engaged in repetitive sexual offending before their adjudication, and to use it as a classification criterion to examine the differential characteristics associated with each group. Read More

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Significance of and immune-related genes to prostate cancer.

Transl Androl Urol 2021 Apr;10(4):1754-1768

Department of Urology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Background: Prostate cancer (PCa) is one of most common male neoplasms. is the tumor suppressor gene with the highest correlation with human tumorigenesis discovered so far. Besides the TP53, immune-related genes attracted much attention since the clinical application of PD-1/PD-L1 (programmed death 1/programmed cell death-ligand 1) related drugs. Read More

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Single-Cell Transcriptomics: Current Methods and Challenges in Data Acquisition and Analysis.

Front Neurosci 2021 22;15:591122. Epub 2021 Apr 22.

Department of Computer Sciences, Baba Ghulam Shah Badshah University, Rajouri, India.

Rapid cost drops and advancements in next-generation sequencing have made profiling of cells at individual level a conventional practice in scientific laboratories worldwide. Single-cell transcriptomics [single-cell RNA sequencing (SC-RNA-seq)] has an immense potential of uncovering the novel basis of human life. The well-known heterogeneity of cells at the individual level can be better studied by single-cell transcriptomics. Read More

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Multichannel Immunosensor Platform for the Rapid Detection of SARS-CoV-2 and Influenza A(H1N1) Virus.

ACS Appl Mater Interfaces 2021 May 9. Epub 2021 May 9.

Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing 100069, P. R. China.

The coronavirus disease 2019 (COVID-19) can present a similar syndrome to an influenza infection, which may complicate diagnosis and clinical management of these two important respiratory infectious diseases, especially during the peak season of influenza. A rapid and convenient point-of-care test (POCT) for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza virus is of great importance for prompt and efficient control of these respiratory epidemics. Herein, a multichannel electrochemical immunoassay (MEIA) platform was developed based on a disposable screen-printed carbon electrode (SPCE) array for the on-site detection of SARS-CoV-2 and A(H1N1). Read More

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Timing and Clinical Significance of Fluid Overload in Pediatric Acute Respiratory Distress Syndrome.

Pediatr Crit Care Med 2021 May 10. Epub 2021 May 10.

Department of General Pediatrics, Children's Hospital of Philadelphia, Philadelphia, PA. Division of Pediatric Critical Care Medicine, Department of Pediatrics and Public Health Science, Penn State Hershey Children's Hospital, Hershey, PA. Department of Anesthesiology and Critical Care Medicine, Children's Hospital of Philadelphia and University of Pennsylvania, Philadelphia, PA.

Objectives: Fluid overload is associated with worse outcomes in adult and pediatric acute respiratory distress syndrome. However, the time-course of fluid overload and its relationship to outcome has not been described. We aimed to determine the relationship between the timing of fluid overload and outcomes over the first 7 days after acute respiratory distress syndrome onset in children. Read More

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Lefort II distraction with zygomatic repositioning versus Lefort III distraction: A comparison of surgical outcomes and complications.

J Craniomaxillofac Surg 2021 Apr 28. Epub 2021 Apr 28.

Craniofacial Center, Seattle Children's Hospital, 4800 Sand Point Way NE, Seattle, WA, 98105, USA. Electronic address:

The aim of the study was to determine if the additional surgical complexity of Lefort II distraction with zygomatic repositioning (LF2ZR) results in increased complications compared to Lefort III distraction (LF3). A retrospective review was performed of all LF3 and LF2ZR advancements performed by the senior author over 15 years. Demographic, operative, postoperative, and cephalometric data were collected from initial procedure through greater than 1 year postoperatively. Read More

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Co-evolution of tumor and immune cells during progression of multiple myeloma.

Nat Commun 2021 May 7;12(1):2559. Epub 2021 May 7.

Department of Medicine, Washington University in St. Louis, St. Louis, MO, USA.

Multiple myeloma (MM) is characterized by the uncontrolled proliferation of plasma cells. Despite recent treatment advances, it is still incurable as disease progression is not fully understood. To investigate MM and its immune environment, we apply single cell RNA and linked-read whole genome sequencing to profile 29 longitudinal samples at different disease stages from 14 patients. Read More

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Older-onset levodopa-responsive parkinsonism with normal DAT-SPECT and pterin hypometabolism.

BMJ Case Rep 2021 May 7;14(5). Epub 2021 May 7.

Department of Neurology, National Hospital Organization Utano National Hospital, Kyoto, Japan.

Pterin species participate in dopamine biosynthesis, and abnormal pteridine metabolism contributes to reduced dopamine. GTP cyclohydrolase 1 (GCH-1) deficiency, which triggers pteridine hypometabolism and normally develops in childhood, can mediate an adult-onset decrease in levodopa production and dopa-responsive dystonia (DRD), with normal dopamine transporter single-photon emission computed tomography (DAT-SPECT). A recent study described normal DAT-SPECT in adult-onset cases with GCH-1 mutations, clinically diagnosed with Parkinson's disease, which raises the possibility that the abnormal metabolism of pteridine may be a differential diagnosis for adult-onset parkinsonism. Read More

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Single-cell Transcriptomics Uncover a Novel Role of Myeloid Cells and T-lymphocytes in the Fibrotic Microenvironment in Peyronie's Disease.

Eur Urol Focus 2021 May 4. Epub 2021 May 4.

Laboratory of Experimental Urology, Department of Development and Regeneration, KU Leuven, Leuven, Belgium; Department of Urology, University Hospitals Leuven, Leuven, Belgium.

Background: Peyronie's disease (PD) is an acquired fibrotic disease affecting the penile tunica albuginea that can lead to curvature and deformities, shortening, and erectile dysfunction. Although immunological mechanisms have been suggested for the pathophysiology of PD, these have not been investigated using single-cell transcriptomics.

Objective: To investigate the immunological signature of plaques from PD patients using immunohistochemistry (IHC) and single-cell RNA sequencing (scRNA-Seq). Read More

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Electrochemical aptasensor for 17β-estradiol using disposable laser scribed graphene electrodes.

Biosens Bioelectron 2021 Apr 28;185:113247. Epub 2021 Apr 28.

Polymer Biointerface Centre, School of Chemical Sciences, The University of Auckland, Private Bag 92019, Auckland, 1142, New Zealand; MacDiarmid Institute for Advanced Materials and Nanotechnology, Victoria University of Wellington, PO Box 600, Wellington, New Zealand. Electronic address:

17β-Estradiol (E2), the strongest of the three major physiological estrogens in females, is an important factor in the female reproductive system. The abnormal level of E2 causes health issues, such as weak bones, urinary tract infections and even depression. Here, we present a novel, sensitive and selective, electrochemical aptasensor for detection of 17β-estradiol (E2). Read More

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Electrospun fibers for vaginal administration of tenofovir disoproxil fumarate and emtricitabine in the context of topical pre-exposure prophylaxis.

J Control Release 2021 May 4. Epub 2021 May 4.

i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal; INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal; CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Gandra, Portugal. Electronic address:

Women are particularly vulnerable to sexual HIV-1 transmission. Oral pre-exposure prophylaxis (PrEP) with tenofovir disoproxil fumarate and emtricitabine (TDF/FTC) is highly effective in avoiding new infections in men, but protection has only been shown to be moderate in women. Such differences have been associated, at least partially, to poor drug penetration of the lower female genital tract and the need for strict adherence to continuous daily oral intake of TDF/FTC. Read More

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Persistent effects of pair bonding in lung cancer cell growth in monogamous .

Elife 2021 May 7;10. Epub 2021 May 7.

Department of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South Carolina, Columbia, United States.

Epidemiological evidence suggests that social interactions and especially bonding between couples influence tumorigenesis, yet whether this is due to lifestyle changes, homogamy (likelihood of individuals to marry people of similar health), or directly associated with host-induced effects in tumors remains debatable. In the present study, we explored if tumorigenesis is associated with the bonding experience in monogamous rodents at which disruption of pair bonds is linked to anxiety and stress. Comparison of lung cancer cell spheroids that formed in the presence of sera from bonded and bond-disrupted deer mice showed that in monogamous and , but not in polygamous , the disruption of pair bonds altered the size and morphology of spheroids in a manner that is consistent with the acquisition of increased oncogenic potential. Read More

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Parameters associated with diagnosis of COVID-19 in emergency department.

Immun Inflamm Dis 2021 May 7. Epub 2021 May 7.

Department of Medicine and Science of Aging, Clinic of Infectious Diseases, University "G. d'Annunzio" of Chieti-Pescara, Chieti, Italy.

Objectives: We designed this study to identify laboratory and radiological parameters, which could be useful to guide the clinician, in the evaluation of a suspected case of coronavirus disease 19 (COVID-19).

Methods: This retrospective, observational, single-center-study recruited patients with a suspect of COVID-19 data were extracted from electronic medical records using a standardized data collection form.

Results: A total of 566 patients with suspect COVID-19 infection were enrolled (280 were COVID-19+). Read More

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To hum or not to hum: Neural transcriptome signature of male courtship vocalization in a teleost fish.

Genes Brain Behav 2021 May 7:e12740. Epub 2021 May 7.

Department of Neurobiology and Behavior, Cornell University, Ithaca, New York, United States.

For many animal species, vocal communication is a critical social behavior and often a necessary component of reproductive success. Additionally, vocalizations are often demanding motor acts. Wanting to know whether a specific molecular toolkit might be required for vocalization, we used RNA-sequencing to investigate neural gene expression underlying the performance of an extreme vocal behavior, the courtship hum of the plainfin midshipman fish (Porichthys notatus). Read More

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DebarcodeR Increases Fluorescent Cell Barcoding Capacity and Accuracy.

Cytometry A 2021 May 7. Epub 2021 May 7.

Department of Cell & Developmental Biology, Vanderbilt University, Nashville, TN, USA.

Fluorescent cell barcoding (FCB) enables efficient collection of tens to hundreds of flow cytometry samples by covalently marking cells with varying concentration of spectrally distinct dyes. A key consideration in FCB is to balance the density of dye barcodes, the complexity of cells in the sample, and the desired accuracy of the debarcoding. Unfortunately, barcoding bench and computational methods have not benefited from the high dimensional revolution in cytometry due to a lack of automated computational tools that effectively balance these common cytometry needs. Read More

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Organ geometry channels reproductive cell fate in the Arabidopsis ovule primordium.

Elife 2021 May 7;10. Epub 2021 May 7.

Plant and Microbial Biology, University of Zürich, Zürich, Switzerland.

In multicellular organisms, sexual reproduction requires the separation of the germline from the soma. In flowering plants, the female germline precursor differentiates as a single spore mother cell (SMC) as the ovule primordium forms. Here, we explored how organ growth contributes to SMC differentiation. Read More

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Multicomponent crystalline solid forms of aripiprazole produced via hot melt extrusion techniques: An exploratory study.

J Drug Deliv Sci Technol 2021 Jun 20;63. Epub 2021 Apr 20.

Department of Pharmaceutics and Drug Delivery, School of Pharmacy, The University of Mississippi, University, MS 38677, USA.

Multicomponent crystalline solid forms (salts, cocrystals and eutectics) are a promising means of enhancing the dissolution behavior of poorly soluble drugs. The present study demonstrates the development of multicomponent solid forms of aripiprazole (ARP) prepared with succinic acid (SA) and nicotinamide (NA) as coformers using the hot melt extrusion (HME) technique. The HME-processed samples were characterized and analyzed using differential scanning calorimetry (DSC), hot stage microscopy (HSM), Fourier transform infrared (FTIR) spectroscopy, powder X-ray diffraction (PXRD) and scanning electron microscopy (SEM). Read More

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Single cell transcriptional zonation of human psoriasis skin identifies an alternative immunoregulatory axis conducted by skin resident cells.

Cell Death Dis 2021 May 6;12(5):450. Epub 2021 May 6.

Division of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Solnavägen 9, 17165, Solna, Stockholm, Sweden.

Psoriasis is the most common skin disease in adults. Current experimental and clinical evidences suggested the infiltrating immune cells could target local skin cells and thus induce psoriatic phenotype. However, recent studies indicated the existence of a potential feedback signaling loop from local resident skin cells to infiltrating immune cells. Read More

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