37,112 results match your criteria signal recognition

Programmable, Multiplexed DNA Circuits Supporting Clinically Relevant, Electrochemical Antibody Detection.

ACS Sens 2021 Jun 15. Epub 2021 Jun 15.

Department of Chemical Science and Technologies, University of Rome, Tor Vergata, 00133 Rome, Italy.

Current health emergencies have highlighted the need to have rapid, sensitive, and convenient platforms for the detection of specific antibodies. In response, we report here the design of an electrochemical DNA circuit that responds quantitatively to multiple specific antibodies. The approach employs synthetic antigen-conjugated nucleic acid strands that are rationally designed to induce a strand displacement reaction and release a redox reporter-modified strand upon the recognition of a specific target antibody. Read More

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Homogeneous Visual and Fluorescence Detection of Circulating Tumor Cells in Clinical Samples Selective Recognition Reaction and Enzyme-Free Amplification.

ACS Nano 2021 Jun 15. Epub 2021 Jun 15.

Department of Laboratory Medicine, State Key Laboratory of Biotherapy and Cancer Center, Med+X Center for Manufacturing, West China Precision Medicine Industrial Technology Institute, Department of Urology, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, Sichuan 610041, China.

Here we report a simple all-nucleic-acid enzyme-free catalyzed hairpin assembly assisted amplification strategy with quantum dots (QDs) as the nanoscale signal reporter for homogeneous visual and fluorescent detection of A549 lung cancer cells from clinical blood samples. This work was based on the phenomenon that CdTe QDs can selectively recognize Ag and C-Ag-C and by using mucin 1 as the circulating tumor cells (CTCs) marker and aptamer as the recognition probe. Under optimized conditions, the limits of detections as low as 0. Read More

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Maple syrup urine disease: magnetic resonance imaging findings in three patients.

J Pak Med Assoc 2021 Apr;71(4):1309-1313

Department of Paediatrics and Child Health, Aga Khan University, Karachi, Pakistan.

Maple syrup urine disease (MSUD) is an autosomal recessive inherited metabolic disorder, caused by branched-chain alpha-ketoacid dehydrogenase (BCKD) deficiency, leading to toxic accumulation of branched-chain amino acids (BCAAs) including leucine, isoleucine and valine and their corresponding a-ketoacids. The diagnosis of MSUD is based on elevated BCAAs and allo-isoleucine in plasma, and branched-chain hydroxyacids and ketoacids in urine. The identification of alloisoleucine >5 µmol/L is considered pathognomonic. Read More

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A serological aptamer-assisted proximity ligation assay for COVID-19 diagnosis and seeking neutralizing aptamers.

Chem Sci 2020 Oct 12;11(44):12157-12164. Epub 2020 Oct 12.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University Nanjing 210023 China

Rapid and accurate diagnosis of COVID-19 plays an essential role in the current epidemic prevention and control. Despite the promise of nucleic acid and antibody tests, there is still a great challenge to reduce the misdiagnosis, especially for asymptomatic individuals. Here we report a generalizable method for highly specific and ultrasensitive detection of serum COVID-19-associated antigens based on an aptamer-assisted proximity ligation assay. Read More

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October 2020

Expression-EEG Bimodal Fusion Emotion Recognition Method Based on Deep Learning.

Comput Math Methods Med 2021 25;2021:9940148. Epub 2021 May 25.

Department of Electrical & Information Engineering, Sichuan Engineering Technical College, Deyang, Sichuan 618000, China.

As one of the key issues in the field of emotional computing, emotion recognition has rich application scenarios and important research value. However, the single biometric recognition in the actual scene has the problem of low accuracy of emotion recognition classification due to its own limitations. In response to this problem, this paper combines deep neural networks to propose a deep learning-based expression-EEG bimodal fusion emotion recognition method. Read More

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Cochlear T2 Signal May Predict Hearing Outcomes After Resection of Acoustic Neuroma.

Otol Neurotol 2021 Jun 11. Epub 2021 Jun 11.

Vanderbilt University Medical Center University of California San Diego School of Medicine, La Jolla, California.

Objective: Examine the impact of preoperative cochlear Fast Imaging Employing Steady-state Acquisition (FIESTA) and Constructive Interference in Steady State (CISS) signal intensity on hearing outcomes after middle cranial fossa (MCF) resection of acoustic neuroma (AN).

Methods: Adult patients (≥18 years) who underwent MCF AN resection for hearing preservation (HP) between November 2017 and September 2019 were retrospectively reviewed. All patients had preoperative word recognition score (WRS) ≥50%. Read More

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Metabolic reprogramming by traditional Chinese medicine and its role in effective cancer therapy.

Pharmacol Res 2021 Jun 10:105728. Epub 2021 Jun 10.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Integration of Chinese and Western Medicine, Peking University Cancer Hospital and Institute, Beijing 100142, PR China. Electronic address:

Metabolic reprogramming, characterized by alterations of cellular metabolic patterns, is fundamentally important in supporting the malignant behaviors of cancer cells. It is considered as a promising therapeutic target against cancer. Traditional Chinese medicine (TCM) and its bioactive components have been used in cancer therapy for an extended period, and they are well-known for their multi-target pharmacological functions and fewer side effects. Read More

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Sampling rate influences the regularity analysis of temporal domain measures of walking more than spatial domain measures.

Gait Posture 2021 Jun 4;88:216-220. Epub 2021 Jun 4.

Center for Research in Human Movement Variability, Department of Biomechanics, University of Nebraska at Omaha, 6160 University Drive, Omaha, NE, 68182-0860, United States; VA Nebraska-Western Iowa Health Care System, VA Research Service, 4104 Woolworth Avenue (112), Omaha, NE, 68105, United States; Department of Health and Kinesiology, Texas A&M University, 2929 Research Parkway, College Station, TX, 77843, United States. Electronic address:

Background: The spatiotemporal dynamics of stepping can provide useful information about walking performance. Most often, the identification of gait motion is performed using 3-D cinematography. The sampling rate of motion capture systems may influence the accuracy of these measures albeit in varying degrees for measures within the spatial versus temporal domain. Read More

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Lineage-specific gene evolution of innate immunity in Bombyx mori to adapt to challenge by pathogens, especially entomopathogenic fungi.

Dev Comp Immunol 2021 Jun 10;123:104171. Epub 2021 Jun 10.

Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China; Sericulture Research Center, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China. Electronic address:

Bombyx mori is a model species of Lepidoptera, in which 21 gene families and 220 genes have been identified as involved in immunity. However, only 45 B. mori - Drosophila melanogaster - Anopheles gambiae - Apis mellifera - Tribolium castaneum 1:1:1:1:1 orthologous genes were identified. Read More

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Design and Engineering of Hypoxia and Acidic pH Dual-Stimuli-Responsive Intelligent Fluorescent Nanoprobe for Precise Tumor Imaging.

Small 2021 Jun 12:e2100243. Epub 2021 Jun 12.

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Institute of Chemical Biology and Nanomedicine (ICBN), Hunan University, Changsha, 410082, China.

Stimulus-responsive fluorescence imaging modality shows great promise for detection of tumor due to the advantages of high sensitivity, simplicity and noninvasiveness. However, some non-cancer regions including nodules and inflammation may also exhibit a stimulus-related characteristic, which cause the problem of nonspecific responsiveness and then cause "false positive" results for tumor recognition. Herein, hypoxia and acidic pH, two typical features strongly associated with tumor invasion, progression and metastasis in tumor microenvironment (TME), are chosen as dual stimuli to fabricate "dual lock-and-key" fluorescent nanoprobe for highly specific and precise imaging of tumor cells. Read More

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Archaeal SRP RNA and SRP19 facilitate the assembly of SRP54-FtsY targeting complex.

Biochem Biophys Res Commun 2021 Jun 8;566:53-58. Epub 2021 Jun 8.

Department of Biochemistry, Bose Institute, Kolkata, India. Electronic address:

The signal recognition particle (SRP) plays an essential role in protein translocation across biological membranes. Stable complexation of two GTPases in the signal recognition particle (SRP) and its receptor (SR) control the delivery of nascent polypeptide to the membrane translocon. In archaea, protein targeting is mediated by the SRP54/SRP19/7S RNA ribonucleoprotein complex (SRP) and the FtsY protein (SR). Read More

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A role for the Cockayne Syndrome B (CSB)-Elongin ubiquitin ligase complex in signal-dependent RNA polymerase II transcription.

J Biol Chem 2021 Jun 8:100862. Epub 2021 Jun 8.

Stowers Institute for Medical Research, Kansas City, MO 64110; Department of Biochemistry & Molecular Biology, University of Kansas Medical Center. Electronic address:

The Elongin complex was originally identified as an RNA polymerase II (RNAPII) elongation factor and subsequently as the substrate recognition component of a Cullin-RING E3 ubiquitin ligase. More recent evidence indicates the Elongin ubiquitin ligase assembles with the Cockayne syndrome B helicase (CSB) in response to DNA damage and can target stalled polymerases for ubiquitylation and removal from the genome. In this report, we present evidence that the CSB-Elongin ubiquitin ligase pathway has roles beyond the DNA damage response in the activation of RNAPII-mediated transcription. Read More

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Self-Assembled Growing DNA Tree Mediated by Exosomes for Amplified Imaging of Messenger RNA in Living Cells.

Anal Chem 2021 Jun 11. Epub 2021 Jun 11.

Department of Clinical Laboratory Medicine, Shanghai Tenth People's Hospital of Tongji University, Shanghai 200072, P. R. China.

Sensitive, accurate, and nondestructive probing of endogenous messenger RNA (mRNA) in living cells places extremely high demands on nanocarriers and probes and is still a challenge. In the present study, we describe a target-triggered self-assembled DNA tree for amplified analysis of mRNA in intact living cells. The probes assembled into a DNA tree are transported into cells by exosomes, which is beneficial for reducing cell damage and realizing nondestructive analysis. Read More

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Noncanonical Functions and Cellular Dynamics of the Mammalian Signal Recognition Particle Components.

Front Mol Biosci 2021 25;8:679584. Epub 2021 May 25.

School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW, Australia.

The signal recognition particle (SRP) is a ribonucleoprotein complex fundamental for co-translational delivery of proteins to their proper membrane localization and secretory pathways. Literature of the past two decades has suggested new roles for individual SRP components, 7SL RNA and proteins SRP9, SRP14, SRP19, SRP54, SRP68 and SRP72, outside the SRP cycle. These noncanonical functions interconnect SRP with a multitude of cellular and molecular pathways, including virus-host interactions, stress response, transcriptional regulation and modulation of apoptosis in autoimmune diseases. Read More

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Beam pen lithography as a new tool for spatially controlled photochemistry, and its utilization in the synthesis of multivalent glycan arrays.

Chem Sci 2014 May 4;5(5):2023-2030. Epub 2014 Feb 4.

Department of Chemistry, University of Miami, Coral Gables, FL 33146, USA.

Herein, we describe how cantilever-free scanning probes can be used to deposit precursor material and subsequently irradiate the precursor to initiate polymerization, resulting in a 3D lithographic method wherein the position, height and diameter of each feature can be tuned independently. Specifically, acrylate and methacrylate monomers were patterned onto thiol terminated glass and subsequently exposed to UV light produced brush polymers by a photoinduced radical acrylate polymerization reaction. Here, we report the first examples of glycan arrays, comprised of methacrylate brush polymers that are side-chain functionalized with α-glucose, by this new lithographic approach. Read More

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Global developmental delay, systemic dysmorphism and epilepsy in a patient with a de novo U2AF2 variant.

J Hum Genet 2021 Jun 11. Epub 2021 Jun 11.

Department of Biochemistry, Hamamatsu University School of Medicine, Hamamatsu, Japan.

U2 small nuclear RNA auxiliary factor 2 (U2AF2) is an essential pre-mRNA splicing factor in an early step of splicing. Alternative splicing plays an important role in neuronal development, and disorders of RNA processing steps are implicated in neurological disorders. Recently, the large trio whole-exome sequencing study reported U2AF2 as a novel gene significantly associated with developmental disorders: however, the clinical details of patients with U2AF2 variants were not available. Read More

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A Fluorescence Assay for Exosome Detection Based on Bivalent Cholesterol Anchor Triggered Target Conversion and Enzyme-Free Signal Amplification.

Anal Chem 2021 Jun 10. Epub 2021 Jun 10.

CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Shandong Key Laboratory of Coastal Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.

Exosomes are emerging as one of the most promising biomarkers for early disease diagnosis and prognosis. The significant challenges facing the available methods include improving the detection specificity and sensitivity in complex biological samples. Herein, a fluorescence assay was established based on a combination of immunomagnetic separation and a two-step signal amplification strategy for direct isolation and subsequent detection of exosomes. Read More

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A linearly extendible multi-artifact removal approach for improved upper extremity EEG-based motor imagery decoding.

J Neural Eng 2021 Jun 10. Epub 2021 Jun 10.

Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences, 1068 Xueyuan Avenue, University Town, Xili, Nanshan, Shenzhen, Guangdong, Shenzhen, Guangdong, 518055, CHINA.

Background And Objective: Non-invasive multichannel Electroencephalography (EEG) recordings provide an alternative source of neural information from which motor imagery (MI) patterns associated with limb movement intent can be decoded for use as control inputs for rehabilitation robots. The presence of multiple inherent dynamic artifacts in EEG signals, however, poses processing challenges for brain-computer interface (BCI) systems. A large proportion of the existing EEG signal preprocessing methods focus on isolating single artifact per time from an ensemble of EEG trials and require calibration and/or reference electrodes, resulting in increased complexity of their application to MI-EEG controlled rehabilitation devices in practical settings. Read More

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Testing the foundations of signal detection theory in recognition memory.

Psychol Rev 2021 Jun 10. Epub 2021 Jun 10.

Department of Experimental Psychology.

Signal detection theory (SDT) plays a central role in the characterization of human judgments in a wide range of domains, most prominently in recognition memory. But despite its success, many of its fundamental properties are often misunderstood, especially when it comes to its testability. The present work examines five main properties that are characteristic of existing SDT models of recognition memory: (a) random-scale representation, (b) latent-variable independence, (c) likelihood-ratio monotonicity, (d) ROC function asymmetry, and (e) nonthreshold representation. Read More

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Developing a Peak Extraction and Retention (PEER) Algorithm for Improving the Temporal Resolution of Raman Spectroscopy.

Anal Chem 2021 Jun 10. Epub 2021 Jun 10.

State Key Laboratory for Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian 361005, China.

In spectroscopic analysis, push-to-the-limit sensitivity is one of the important topics, particularly when facing the qualitative and quantitative analyses of the trace target. Normally, the effective recognition and extraction of weak signals are the first key steps, for which there has been considerable effort in developing various denoising algorithms for decades. Nevertheless, the lower the signal-to-noise ratio (SNR), the greater the deviation of the peak height and shape during the denoising process. Read More

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Derived loss of signal complexity and plasticity in a genus of weakly electric fish.

J Exp Biol 2021 Jun 10. Epub 2021 Jun 10.

Department of Biology, University of Oklahoma, Norman, OK, 73019, USA.

Signal plasticity can maximize the usefulness of costly animal signals such as the electric organ discharges (EODs) of weakly electric fishes. Some species of the order Gymnotiformes rapidly alter their EOD amplitude and duration in response to circadian cues and social stimuli. How this plasticity is maintained across related species with different degrees of signal complexity is poorly understood. Read More

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T2-mapping increase is the prevalent imaging biomarker of myocardial involvement in active COVID-19: a Cardiovascular Magnetic Resonance study.

J Cardiovasc Magn Reson 2021 06 10;23(1):68. Epub 2021 Jun 10.

Department of Radiological, Oncological and Pathological Sciences, "Sapienza" University of Rome, Viale del Policlinico 155, 00161, Rome, Italy.

Background: Early detection of myocardial involvement can be relevant in coronavirus disease 2019 (COVID-19) patients to timely target symptomatic treatment and decrease the occurrence of the cardiac sequelae of the infection. The aim of the present study was to assess the clinical value of cardiovascular magnetic resonance (CMR) in characterizing myocardial damage in active COVID-19 patients, through the correlation between qualitative and quantitative imaging biomarkers with clinical and laboratory evidence of myocardial injury.

Methods: In this retrospective observational cohort study, we enrolled 27 patients with diagnosis of active COVID-19 and suspected cardiac involvement, referred to our institution for CMR between March 2020 and January 2021. Read More

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Known sequence features can explain half of all human gene ends.

NAR Genom Bioinform 2021 Jun 4;3(2):lqab042. Epub 2021 Jun 4.

Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada.

Cleavage and polyadenylation (CPA) sites define eukaryotic gene ends. CPA sites are associated with five key sequence recognition elements: the upstream UGUA, the polyadenylation signal (PAS), and U-rich sequences; the CA/UA dinucleotide where cleavage occurs; and GU-rich downstream elements (DSEs). Currently, it is not clear whether these sequences are sufficient to delineate CPA sites. Read More

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Development of High Accuracy Classifier for the Speaker Recognition System.

Appl Bionics Biomech 2021 19;2021:5559616. Epub 2021 May 19.

Faculty of Engineering, Altinbas University, Istanbul 34676, Turkey.

Speech signal is enriched with plenty of features used for biometrical recognition and other applications like gender and emotional recognition. Channel conditions manifested by background noise and reverberation are the main challenges causing feature shifts in the test and training data. In this paper, a hybrid speaker identification model for consistent speech features and high recognition accuracy is made. Read More

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Diagnosis of extracranial carotid stenosis by MRA of the brain.

Sci Rep 2021 Jun 8;11(1):12010. Epub 2021 Jun 8.

Department of Neurology, Neurological Institute, Taipei Veterans General Hospital, Taipei, Taiwan.

Severe extracranial carotid stenosis (SECS) patients may present with nonspecific neurological symptoms that require intracranial magnetic resonance imaging (MRI) and time-of-flight (TOF)-MR angiography (MRA) to exclude intracranial pathology. Recognition of SECS on intracranial TOF-MRA findings is beneficial to provide a prompt carotid imaging study and aggressive stroke prevention. Patients with SECS (January 2016 to May 2019) undergoing percutaneous transluminal angioplasty and stenting (PTAS) were included. Read More

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The Inhibitory Effect of Emotional Conflict Control on Memory Retrieval.

Neuroscience 2021 Jun 5. Epub 2021 Jun 5.

Department of Rehabilitation, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China. Electronic address:

Evidence is mounting that emotional conflict is mainly resolved by the rostral anterior cingulate inhibiting the processing of emotional distractors. However, this theory has not been verified from the perspective of memory retrieval. This experiment aimed to explore the offline effect of emotional conflict processing on memory retrieval. Read More

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Evolutionary crossroads of cell signaling: PP1 and PP2A substrate sites in intrinsically disordered regions.

Biochem Soc Trans 2021 Jun 8. Epub 2021 Jun 8.

Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestraße 18, 79104 Freiburg, Germany.

Phosphorylation of the hydroxyl group of the amino acids serine and threonine is among the most prevalent post-translational modifications in mammalian cells. Phospho-serine (pSer) and -threonine (pThr) represent a central cornerstone in the cell's toolbox for adaptation to signal input. The true power for the fast modulation of the regulatory pSer/pThr sites arises from the timely attachment, binding and removal of the phosphate. Read More

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An NIR dual-emitting/absorbing inorganic compact pair: A self-calibrating LRET system for homogeneous virus detection.

Biosens Bioelectron 2021 May 29;190:113369. Epub 2021 May 29.

Molecular Recognition Research Center, Korea Institute of Science and Technology (KIST), Seoul, 02792, South Korea; Department of Chemistry, Hanyang University, Seoul, 04763, Republic of Korea. Electronic address:

Many conventional optical biosensing systems use a single responsive signal in the visible light region. This limits their practical applications, as the signal can be readily perturbed by various external environmental factors. Herein, a near-infrared (NIR)-based self-calibrating luminescence resonance energy transfer (LRET) system was developed for background-free detection of analytes in homogeneous sandwich-immunoassays. Read More

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Ensemble Support Vector Recurrent Neural Network for Brain Signal Detection.

IEEE Trans Neural Netw Learn Syst 2021 Jun 7;PP. Epub 2021 Jun 7.

The brain-computer interface (BCI) P300 speller analyzes the P300 signals from the brain to achieve direct communication between humans and machines, which can assist patients with severe disabilities to control external machines or robots to complete expected tasks. Therefore, the classification method of P300 signals plays an important role in the development of BCI systems and technologies. In this article, a novel ensemble support vector recurrent neural network (E-SVRNN) framework is proposed and developed to acquire more accurate and efficient electroencephalogram (EEG) signal classification results. Read More

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Ultrasensitive Detection of Bacteria Using a 2D MOF Nanozyme-Amplified Electrochemical Detector.

Anal Chem 2021 Jun 7. Epub 2021 Jun 7.

State Key Laboratory of Analytical Chemistry for Life Science; School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

Bacterial infection is one of the major causes of human death worldwide. To prevent bacterial infectious diseases from spreading, it is of critical importance to develop convenient, ultrasensitive, and cost-efficient methods for bacteria detection. Here, an electrochemical detector of a functional two-dimensional (2D) metal-organic framework (MOF) nanozyme was developed for the sensitive detection of pathogenic . Read More

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