72 results match your criteria shell trehalose


Leucine enhances the dispersibility of trehalose-containing spray-dried powders on exposure to a high-humidity environment.

Int J Pharm 2021 Mar 31;601:120561. Epub 2021 Mar 31.

Department of Mechanical Engineering, University of Alberta, Edmonton, Alberta, Canada. Electronic address:

This study investigates the ability of various shell-forming excipients to preserve the dispersibility of dry powder dosage forms, e.g., nasally administered vaccines, upon exposure to a high-humidity environment. Read More

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Neutron total scattering investigation on the dissolution mechanism of trehalose in NaOH/urea aqueous solution.

Struct Dyn 2021 Jan 10;8(1):014901. Epub 2021 Feb 10.

Institute for Advanced Study, Shenzhen University, Shenzhen 508060, China.

Trehalose is chosen as a model molecule to investigate the dissolution mechanism of cellulose in NaOH/urea aqueous solution. The combination of neutron total scattering and empirical potential structure refinement yields the most probable all-atom positions in the complex fluid and reveals the cooperative dynamic effects of NaOH, urea, and water molecules in the dissolution process. NaOH directly interacts with glucose rings by breaking the inter- and intra-molecular hydrogen bonding. Read More

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January 2021

On the particle formation of leucine in spray drying of inhalable microparticles.

Int J Pharm 2021 Jan 21;592:120102. Epub 2020 Nov 21.

Department of Mechanical Engineering, University of Alberta, Edmonton, Alberta, Canada. Electronic address:

The particle formation of L-leucine, a dispersibility-enhancing amino acid used in the spray drying of inhalable pharmaceutical aerosols, was extensively studied using three experimental methods, and the results were interpreted with the aid of theory. A comparative-kinetics electrodynamic balance was used to study the shell formation behavior in single evaporating microdroplets containing leucine and trehalose. Different concentration thresholds of solidification and shell formation were determined for trehalose and leucine, which were then used in the particle formation model to predict the properties of spray-dried particles. Read More

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January 2021

Control of electron transfer by protein dynamics in photosynthetic reaction centers.

Crit Rev Biochem Mol Biol 2020 10 3;55(5):425-468. Epub 2020 Sep 3.

Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, USA.

Trehalose and glycerol are low molecular mass sugars/polyols that have found widespread use in the protection of native protein states, in both short- and long-term storage of biological materials, and as a means of understanding protein dynamics. These myriad uses are often attributed to their ability to form an amorphous glassy matrix. In glycerol, the glass is formed only at cryogenic temperatures, while in trehalose, the glass is formed at room temperature, but only upon dehydration of the sample. Read More

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October 2020

Development of a method to cryopreserve Greenshell mussel™ (Perna canaliculus) veliger larvae.

Cryobiology 2020 10 27;96:37-44. Epub 2020 Aug 27.

Cawthron Institute, 98 Halifax Street East, Private Bag 2, Nelson, 7042, New Zealand.

Cryopreservation of larvae of Greenshell™ mussel Perna canaliculus, the most cultivated species in New Zealand, can provide flexibility for selective breeding programmes and enhance its global production. In this study, we set out to develop a reliable protocol for freezing D-stage larvae of Greenshell™ mussels that ensured long-term survival for successful rearing of thawed larvae in the hatchery. The effects of different combinations of cryoprotecting agents (CPA), varying CPA equilibration times, larval concentrations per straw as well as different larval development stages (48 h vs 72 h old) were evaluated by assessing the behavioural response (swimming activity, algal consumption), shell size and survival of larvae, up to 4 days post-thawing. Read More

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October 2020

Permeabilized TreS-Expressing Cells Decorated with Glucose Isomerase and a Shell of ZIF-8 as a Reusable Biocatalyst for the Coproduction of Trehalose and Fructose.

J Agric Food Chem 2020 Apr 6;68(15):4464-4472. Epub 2020 Apr 6.

College of Food Science and Light Industry, Nanjing Tech University, Nanjing 210009, P. R. China.

Metal-organic frameworks (MOFs) are a class of porous materials with versatile properties. In this study, ZIF-8 was employed to establish a two-enzyme system by encapsulating permeabilized cells coated with glucose isomerase. was constructed by introducing the shuttle plasmid PMA5 associated with the overexpression of trehalose synthase. Read More

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Phenolic composition and nutritional attributes of diaphragma juglandis fructus and shell of walnut ( L.).

Food Sci Biotechnol 2020 Feb 8;29(2):187-196. Epub 2019 Aug 8.

1College of Life Science, Leshan Normal University, Leshan, 614000 People's Republic of China.

Phenolic composition and nutritional attributes of diaphragma juglandis fructus (Djf) and walnut shells (Ws) were investigated. Phenolic acids, hydroxybenzoic acid, isoflavone, and flavone were identified in the free phenolic fractions (FPFs) of both Djf and Ws. Bound phenolic fractions were less than FPFs both in content and diversity. Read More

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February 2020

Trileucine and Pullulan Improve Anti-Campylobacter Bacteriophage Stability in Engineered Spray-Dried Microparticles.

Ann Biomed Eng 2020 Apr 16;48(4):1169-1180. Epub 2019 Dec 16.

Department of Mechanical Engineering, University of Alberta, 10-203 Donadeo Innovation Centre for Engineering, 9211 116th Street NW, Edmonton, AB, T6G 1H9, Canada.

Spray drying biologics into a powder can increase thermal stability and shelf-life relative to liquid formulations, potentially eliminating the need for cold chain infrastructure for distribution in developing countries. In this study, process modelling, microparticle engineering, and a supplemented phase diagram were used to design physically stable fully amorphous spray-dried powder capable of stabilizing biological material. A greater proportion of anti-Campylobacter bacteriophage CP30A remained biologically active after spray drying using excipient formulations containing trehalose and a high glass transition temperature amorphous shell former, either trileucine or pullulan, as compared to the commonly used crystalline shell former, leucine, or a low glass transition temperature amorphous shell former, pluronic F-68. Read More

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Encapsulation of Lactobacillus plantarum in cellulose based microgel with controlled release behavior and increased long-term storage stability.

Carbohydr Polym 2019 Nov 8;223:115065. Epub 2019 Jul 8.

Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology & Business University (BTBU), Beijing, 100048, China; College of Food Science & Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China. Electronic address:

Probiotics are believed to provide benefits to human health; however, a good storage stability is the prerequisite for the probiotic products making function. Herein, we reported a pathway to fabricate the Ca-alginate/cryoprotectants/cellulose composite (ACFP) capsules to protect L. plantarum cells, which showed the minimal loss of viability during the vacuum freeze-drying process. Read More

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November 2019

Characterization of hydration water in supercooled water-trehalose solutions: The role of the hydrogen bonds network.

J Chem Phys 2019 Jul;151(4):044507

Dipartimento di Matematica e Fisica, Università Roma Tre, Via della Vasca Navale 84, 00146 Rome, Italy.

The structural and dynamical properties of hydration water in aqueous solutions of trehalose are studied with molecular dynamics simulation. We simulate the systems in the supercooled region to investigate how the interaction with the trehalose molecules modifies the hydrogen bond network, the structural relaxation, and the diffusion properties of hydration water. The analysis is performed by considering the radial distribution functions, the residence time of water molecules in the hydration shell, the two body excess entropy, and the hydrogen bond water-water and water-trehalose correlations of the hydration water. Read More

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Computational spectroscopy of trehalose, sucrose, maltose, and glucose: A comprehensive study of TDSS, NQR, NOE, and DRS.

J Chem Phys 2019 May;150(17):175102

University of Vienna, Faculty of Chemistry, Department of Computational Biological Chemistry, Währingerstraße 17, A-1090 Vienna, Austria.

The bioprotective nature of monosaccharides and disaccharides is often attributed to their ability to slow down the dynamics of adjacent water molecules. Indeed, solvation dynamics close to sugars is indisputably retarded compared to bulk water. However, further research is needed on the qualitative and quantitative differences between the water dynamics around different saccharides. Read More

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Nonviral Gene Delivery with Cationic Glycopolymers.

Acc Chem Res 2019 05 17;52(5):1347-1358. Epub 2019 Apr 17.

Department of Chemistry , University of Minnesota , 207 Pleasant Street SE , Minneapolis , Minnesota 55455 , United States.

The field of gene therapy, which aims to treat patients by modulating gene expression, has come to fruition and has landed several landmark FDA approvals. Most gene therapies currently rely on viral vectors to deliver nucleic acid cargo into cells, but there is significant interest in moving toward chemical-based methods, such as polymer-based vectors, due to their low cost, immunocompatibility, and tunability. The full potential of polymer-based delivery systems has yet to be realized, however, because most polymeric transfection reagents are either too inefficient or too toxic for use in the clinic. Read More

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Mechanism of Trehalose-Induced Protein Stabilization from Neutron Scattering and Modeling.

J Phys Chem B 2019 05 18;123(17):3679-3687. Epub 2019 Apr 18.

Department of Physics , Chalmers University of Technology , SE-412 96 Göteborg , Sweden.

The sugar molecule trehalose has been proven to be an excellent stabilizing cosolute for the preservation of biological materials. However, the stabilizing mechanism of trehalose has been much debated during the previous decades, and it is still not fully understood, partly because it has not been completely established how trehalose molecules structure around proteins. Here, we present a molecular model of a protein-water-trehalose system, based on neutron scattering results obtained from neutron diffraction, quasielastic neutron scattering, and different computer modeling techniques. Read More

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Hydrodynamic volume of trehalose and its water uptake mechanism.

Biophys Chem 2019 06 2;249:106145. Epub 2019 Apr 2.

KN Toosi University, Tehran, Iran.

Trehalose ability to preserve water in biology has spawned research on this special disaccharide and its solutions. Trehalose unlike any other disaccharide, tend to mix with almost any amount of water. In water, Trehalose forms a hydrodynamic volume with bound waters (both coordination water and semicircular heterogeneities), capable of perturbing the very nature of normal bulk water. Read More

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Particle Surface Roughness Improves Colloidal Stability of Pressurized Pharmaceutical Suspensions.

Pharm Res 2019 Jan 30;36(3):43. Epub 2019 Jan 30.

Department of Mechanical Engineering, 10-269 Donadeo Innovation Centre for Engineering, University of Alberta, Edmonton, Alberta, T6G 1H9, Canada.

Purpose: The effects of particle size and particle surface roughness on the colloidal stability of pressurized pharmaceutical suspensions were investigated using monodisperse spray-dried particles.

Methods: The colloidal stability of multiple suspensions in the propellant HFA227ea was characterized using a shadowgraphic imaging technique and quantitatively compared using an instability index. Model suspensions of monodisperse spray-dried trehalose particles of narrow distributions (GSD < 1. Read More

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January 2019

Restoration of Myoglobin Native Fold from Its Initial State of Amyloid Formation by Trehalose.

J Phys Chem B 2018 12 30;122(50):11962-11968. Epub 2018 Nov 30.

Institute of Materials Structure Science , High Energy Accelerator Research Organization , 1-1 Oho , Tsukuba , Ibaraki 305-0801 , Japan.

Organisms having tolerances against extreme environments produce and accumulate stress proteins and/or sugars in cells against the extreme environment such as high or low temperature, drying, and so forth. Sugars and/or polyols are known to prevent protein denaturation and enzyme deactivation. In particular, trehalose has received considerable attention because of its association with cryptobiosis and anhydrobiosis. Read More

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December 2018

Protection against Dehydration: A Neutron Diffraction Study on Aqueous Solutions of a Model Peptide and Trehalose.

J Phys Chem B 2018 11 5;122(45):10291-10295. Epub 2018 Nov 5.

Dipartimento di Scienze , Universitá degli Studi Roma Tre , via della Vasca Navale 84 , 00146 Roma , Italy.

The ability of a wide class of organisms to reversibly go through cycles of suspended life and active metabolism, depending on the turnover of drought and normal water availability conditions, represents a challenging issue. The interest in the natural mechanism for drought survival has grown over time along with the request for always more efficient conservation techniques for biological materials. Carbohydrates, such as trehalose, accumulated in the cytoplasm of drought resistant cells, are considered responsible for desiccation tolerance. Read More

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November 2018

Sugar-Mediated Stabilization of Protein against Chemical or Thermal Denaturation.

J Phys Chem B 2018 09 7;122(37):8685-8697. Epub 2018 Sep 7.

Graduate School of Science and Technology , Gunma University , 4-2 Aramaki , Maebashi , Gunma 371-8510 , Japan.

The protective action of sugars against the denaturation of myoglobin was clarified by X-ray and neutron scattering methods. Different types of sugars such as disaccharides (trehalose, sucrose) and monosaccharides (glucose, fructose) were used. Experimental data and theoretical simulation based on three different solvation models (preferential solvation model, nonpreferential solvation model, and preferential exclusion (hydration) model) indicated that sugar molecules were preferentially or weakly excluded from the protein surface and preserved the native protein hydration shell. Read More

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September 2018

Titration of Folate Pathway Enzymes.

Appl Environ Microbiol 2018 10 17;84(19). Epub 2018 Sep 17.

Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, Tennessee, USA

How enzymes behave in cells is likely different from how they behave in the test tube. Previous studies find that osmolytes interact weakly with folate. Removal of the osmolyte from the solvation shell of folate is more difficult than removal of water, which weakens binding of folate to its enzyme partners. Read More

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October 2018

Hydrogen Bond Length as a Key To Understanding Sweetness.

J Phys Chem Lett 2018 Jul 22;9(13):3667-3672. Epub 2018 Jun 22.

Dipartimento di Scienze, Sezione di Nanoscienze , Università degli Studi "Roma Tre" , Via della Vasca Navale 84 , 00146 Roma , Italy.

Neutron diffraction experiments have been performed to investigate and compare the structure of the hydration shell of three monosaccharides, namely, fructose, glucose, and mannose. It is found that despite their differences with respect to many thermodynamical quantities, bioprotective properties against environmental stresses, and taste, the influence of these monosaccharides on the bulk water solvent structure is virtually identical. Conversely, these sugars interact with the neighboring water molecules by forming H bonds of different length and strength. Read More

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Structures of jacalin-related lectin PPL3 regulating pearl shell biomineralization.

Proteins 2018 06 23;86(6):644-653. Epub 2018 Mar 23.

Faculty of Bioscience, Nagahama Institute of Bio-Science and Technology, 1266 Tamura-Cho, Nagahama, Shiga 526-0829, Japan.

The nacreous layer of pearl oysters is one of the major biominerals of commercial and industrial interest. Jacalin-related lectins, including PPL3 isoforms, are known to regulate biomineralization of the Pteria penguin pearl shell, although the molecular mechanisms are largely unknown. The PPL3 crystal structures were determined partly by utilizing microgravity environments for 3 isoforms, namely, PPL3A, PPL3B, and PPL3C. Read More

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Local water sensing: water exchange in bacterial photosynthetic reaction centers embedded in a trehalose glass studied using multiresonance EPR.

Phys Chem Chem Phys 2017 Oct;19(41):28388-28400

Max-Planck-Institut für Chemische Energiekonversion, Stiftstrasse 34-36, D-45470 Mülheim an der Ruhr, Germany.

Using isotope labeled water (DO and HO) and pulsed W-band (94 GHz) high-field multiresonance EPR spectroscopies, such as ELDOR-detected NMR and ENDOR, the biologically important question of detection and quantification of local water in proteins is addressed. A bacterial reaction center (bRC) from Rhodobacter sphaeroides R26 embedded into a trehalose glass matrix is used as a model system. The bRC hosts the two native radical cofactor ions (primary electron donor) and (primary electron acceptor) as well as an artificial nitroxide spin label site-specifically attached to the surface of the H-protein domain. Read More

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October 2017

Preparation of Acid-Resistant Microcapsules with Shell-Matrix Structure to Enhance Stability of Streptococcus Thermophilus IFFI 6038.

J Food Sci 2017 Aug 11;82(8):1978-1984. Epub 2017 Jul 11.

School of Pharmaceutical Sciences, Sun Yat-sen Univ., Guangzhou, 510006, PR, China.

Microencapsulation is an effective technology used to protect probiotics against harsh conditions. Extrusion is a commonly used microencapsulation method utilized to prepare probiotics microcapsules that is regarded as economical and simple to operate. This research aims to prepare acid-resistant probiotic microcapsules with high viability after freeze-drying and optimized storage stability. Read More

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Poly(trehalose) Nanoparticles Prevent Amyloid Aggregation and Suppress Polyglutamine Aggregation in a Huntington's Disease Model Mouse.

ACS Appl Mater Interfaces 2017 Jul 3;9(28):24126-24139. Epub 2017 Jul 3.

Centre for Advanced Materials, Indian Association for the Cultivation of Science , Kolkata-700032, India.

Prevention and therapeutic strategies for various neurodegenerative diseases focus on inhibiting protein fibrillation, clearing aggregated protein plaques from the brain, and lowering protein-aggregate-induced toxicity. We have designed poly(trehalose) nanoparticles that can inhibit amyloid/polyglutamine aggregation under extra-/intracellular conditions, reduce such aggregation-derived cytotoxicity, and prevent polyglutamine aggregation in a Huntington's disease (HD) model mouse brain. The nanoparticles have a hydrodynamic size of 20-30 nm and are composed of a 6 nm iron oxide core and a zwitterionic polymer shell containing ∼5-12 wt % covalently linked trehalose. Read More

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An unexplored remarkable PNIPAM-osmolyte interaction study: An integrated experimental and simulation approach.

J Colloid Interface Sci 2017 Oct 30;504:417-428. Epub 2017 May 30.

Discipline of Pharmaceutical Sciences, School of Health Sciences, University of KwaZulu - Natal (UKZN), Westville Campus, Durban 4000, South Africa.

We investigate the aggregation and collapse of water soluble amphiphilic polymer, poly(N-isopropylacrylamide) (PNIPAM), in aqueous solution containing variable amount of trehalose, sucrose and sorbitol. The effect of these osmolytes on the coil to globular transition of the PNIPAM is studied by the use of comprehensive biophysical techniques like UV-visible spectroscopy, fluorescence spectroscopy, dynamic light scattering and Fourier transform infrared spectroscopy (FTIR). The polarization induced by these additives promotes the collapsed state of PNIPAM at much lower temperature as compared to the pure PNIPAM in aqueous solution. Read More

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October 2017

Revisiting Hydrogen Bond Thermodynamics in Molecular Simulations.

J Chem Theory Comput 2017 Jun 1;13(6):2851-2857. Epub 2017 Jun 1.

Institute of Chemistry and The Fritz Haber Research Center, The Hebrew University , Jerusalem 91904, Israel.

In processes involving aqueous solutions and in almost every biomolecular interaction, hydrogen bonds play important roles. Though weak compared to the covalent bond, hydrogen bonds modify the stability and conformation of numerous small and large molecules and modulate their intermolecular interactions. We propose a simple methodology for extracting hydrogen bond strength from atomistic level simulations. Read More

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Molecular dynamics approach to understand the denaturing effect of a millimolar concentration of dodine on a λ-repressor and counteraction by trehalose.

Phys Chem Chem Phys 2017 May;19(20):13160-13171

Department of Chemistry, Indian Institute of Technology, Guwahati, India.

Commonly used denaturants for protein denaturation are conventionally required in high concentrations in order to produce their effects. In this study, a molecular dynamics simulation of a mutated version of the N-terminal domain of a λ-repressor is carried out in the presence of a 10 millimolar (mM) concentration of dodine. Such a small concentration is found to be effective for denaturation of the protein. Read More

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New insights into the interaction of proteins and disaccharides-The effect of pH and concentration.

Biopolymers 2017 Feb;107(2):39-45

Department of Physics, University of Halle, Halle, 06120, Germany.

To gain new insights into the interaction of proteins and disaccharides, we investigated the hydrodynamic radii, RhProt, of lysozyme molecules in solution and in a ternary protein-sugar-water system by PFG-NMR. Our approach is based on the assumption that the anhydrobiotic properties of disaccharides like trehalose are based on aggregation of sugar molecules to the proteins, i.e. Read More

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February 2017

Two-Dimensional Crystallization of P22 Virus-Like Particles.

J Phys Chem B 2016 07 11;120(26):5938-44. Epub 2016 May 11.

Department of Chemistry, Indiana University , 800 East Kirkwood Avenue, Bloomington, Indiana 47405, United States.

Virus-like particles (VLPs) are well established platforms for constructing functional biomimetic materials. The VLP from the bacteriophage P22 can be used as a nanocontainer to sequester active enzymes, at high concentration, within its cavity through a process of directed self-assembly. Construction of ordered 2D assemblies of these catalytic VLPs can be envisioned as a functional membrane. Read More

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Effect of BBX-B8 overexpression on development, body weight, silk protein synthesis and egg diapause of Bombyx mori.

Transgenic Res 2016 08 7;25(4):507-16. Epub 2016 Mar 7.

School of Biology and Basic Medical Science, Soochow University, Suzhou, 215123, China.

Bombyxin (BBX) is an insulin-like peptide exists in the silkworm Bombyx mori. Our previous studies on the effects of inhibiting BBX-B8 expression found that BBX-B8 is important for the development of organ, reproduction and trehalose metabolism in the silkworms. In this paper, we investigated the expression profile of the BBX-B8 gene and effect of BBX-B8 overexpression on the development, body weight, silk protein synthesis and egg diapause of B. Read More

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