4,060 results match your criteria severely inhibited


Troglitazone inhibits hepatic oval cell proliferation by inducing cell cycle arrest through Hippo/YAP pathway regulation.

Dig Liver Dis 2021 Sep 13. Epub 2021 Sep 13.

Guangdong Second Provincial General Hospital, 466 Middle Xingang Road, Guangzhou, 510317, Guangdong, People's Republic of China. Electronic address:

Hepatic oval cells have strong proliferation and differentiation capabilities and are activated when chronic liver injury occurs or when liver function is severely impaired. Peroxisome proliferation-activated receptors (PPARs) are ligand-dependent, sequence-specific nuclear transcription factors. PPARγ is closely related to liver diseases (such as liver cancer, liver fibrosis and non-alcoholic fatty liver disease). Read More

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September 2021

RS-5645 attenuates inflammatory cytokine storm induced by SARS-CoV-2 spike protein and LPS by modulating pulmonary microbiota.

Int J Biol Sci 2021 25;17(13):3305-3319. Epub 2021 Jul 25.

Shanghai Geriatric Institute of Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200031, China.

An inflammatory cytokine storm is considered an important cause of death in severely and critically ill COVID-19 patients, however, the relationship between the SARS-CoV-2 spike (S) protein and the host's inflammatory cytokine storm is not clear. Here, the qPCR results indicated that S protein induced a significantly elevated expression of multiple inflammatory factor mRNAs in peripheral blood mononuclear cells (PBMCs), whereas RS-5645 ((4-(thiophen-3-yl)-1-(p-tolyl)-1H-pyrrol-3-yl)(3,4,5-trimethoxyphenyl)methanone) attenuated the expression of the most inflammatory factor mRNAs. RS-5645 also significantly reduced the cellular ratios of CD45+/IFNγ+, CD3+/IFNγ+, CD11b+/IFNγ+, and CD56+/IFNγ+ in human PBMCs. Read More

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Fungal Extract of IBRL OS-64 Inhibits the Growth of Skin Pathogenic Bacterium and Attenuates Biofilms of Methicillin-Resistant .

Malays J Med Sci 2021 Aug 26;28(4):24-36. Epub 2021 Aug 26.

Industrial Biotechnology Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, Pulau Pinang, Malaysia.

Background: The emergence of multidrug-resistant pathogens associated with biofilm formation can cause life-threatening infections to humans. Therefore, the present study aims to evaluate the effects of the fungal extract of () Industrial Biotechnology Research Laboratory (IBRL) OS-64 on bacterial cells and the biofilm formation of methicillin-resistant (MRSA).

Methods: Broth microdilution and semi-quantitative adherence assays were conducted to determine the anti-biofilm activity of the fungal extract. Read More

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Inhibition Effect of Phosphorus Poisoning on the Dynamics and Redox of Cu Active Sites in a Cu-SSZ-13 NH-SCR Catalyst for NO Reduction.

Environ Sci Technol 2021 Sep 12. Epub 2021 Sep 12.

Institute of Inorganic Chemistry, RWTH Aachen University, Landoltweg 1a, 52074 Aachen, Germany.

Phosphorus (P) stemming from biodiesel and/or lubricant oil additives is unavoidable in real diesel exhausts and deactivates gradually the Cu-SSZ-13 zeolite catalyst for ammonia-assisted selective catalytic NO reduction (NH-SCR). Here, the deactivation mechanism of Cu-SSZ-13 by P-poisoning was investigated by examination of the structural changes and by probing the dynamics and redox of Cu active sites via a combination of impedance spectroscopy, diffuse reflection infrared Fourier transform spectroscopy, and ultraviolet-visible spectroscopy. We unveiled that strong interactions between Cu and P led to not only a loss of Cu active sites for catalytic turnovers but also a restricted dynamic motion of Cu species during low-temperature NH-SCR catalysis. Read More

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September 2021

Effect of the form of granular sludge and temperature on anammox immobilized fillers: From performance to microbial community analysis.

Sci Total Environ 2021 Aug 24;803:149754. Epub 2021 Aug 24.

Key Laboratory of Beijing for Water Quality Science and Water Environmental Recovery Engineering, College of Architectural Engineering, Beijing University of Technology, Beijing 100124, China.

The immobilized carrier was prepared with complete anaerobic ammonia oxidation granular sludge (AnGS) and crushed AnGS, respectively. We evaluated the effects of granular form and continuous temperature changes on nitrogen removal by immobilized anaerobic ammonium oxidation (anammox) filler. The results showed that the rate of nitrogen removal of crushed and encapsulated AnGS was 20% higher than that of direct encapsulated AnGS. Read More

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is transcriptionally inhibited by through the transcription factor in irradiation-treated HeLa cells.

Toxicol Res (Camb) 2021 Aug 29;10(4):875-884. Epub 2021 Jul 29.

Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Haidian District, Beijing 100850, P. R. China.

Cervical cancer (CC) is a type of pelvic malignant tumor that severely threatens women's health. Current evidence suggests that , as a potential radiosensitizer, promotes irradiation-induced apoptosis in CC tissues in patients undergoing chemoradiotherapy. has been shown to be involved in the G/M-phase transition. Read More

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Response of anaerobic granular sludge to long-term loading of roxarsone: From macro- to micro-scale perspective.

Water Res 2021 Aug 27;204:117599. Epub 2021 Aug 27.

School of Civil Engineering, Hefei University of Technology, Hefei 230009, China; Anhui Provincial Engineering Laboratory for Rural Water Environment and Resources, Hefei University of Technology, Hefei 230009, China. Electronic address:

Extensive use of organoarsenic feed additives such as roxarsone has caused organoarsenicals to occur in livestock wastewater and further within anaerobic wastewater treatment systems. Currently, information on the long-term impacts of roxarsone on anaerobic granular sludge (AGS) activity and the underlying mechanisms is very limited. In this study, the response of AGS to long-term loading of roxarsone was investigated using a laboratory up-flow anaerobic sludge blanket reactor spiked with 5. Read More

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SOX10 ablation severely impairs the generation of postmigratory neural crest from human pluripotent stem cells.

Cell Death Dis 2021 Aug 27;12(9):814. Epub 2021 Aug 27.

VIP Medical Service Center, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Animal studies have indicated that SOX10 is one of the key transcription factors regulating the proliferation, migration and differentiation of multipotent neural crest (NC), and mutation of SOX10 in humans may lead to type 4 Waardenburg syndrome (WS). However, the exact role of SOX10 in human NC development and the underlying molecular mechanisms of SOX10-related human diseases remain poorly understood due to the lack of appropriate human model systems. In this study, we successfully generated SOX10-knockout human induced pluripotent stem cells (SOX10 hiPSCs) by the CRISPR-Cas9 gene editing tool. Read More

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Hyperglycemia alters mitochondrial respiration efficiency and mitophagy in human podocytes.

Exp Cell Res 2021 Oct 23;407(1):112758. Epub 2021 Aug 23.

Laboratory of Molecular and Cellular Nephrology, Mossakowski Medical Research Institute, Polish Academy of Sciences, Wita Stwosza St. 63, 80-308, Gdansk, Poland; Faculty of Chemistry, University of Gdansk, Wita Stwosza St. 63, 80-308, Gdansk, Poland.

Podocytes constitute the outer layer of the renal glomerular filtration barrier. Their energy requirements strongly depend on efficient oxidative respiration, which is tightly connected with mitochondrial dynamics. We hypothesized that hyperglycemia modulates energy metabolism in glomeruli and podocytes and contributes to the development of diabetic kidney disease. Read More

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October 2021

Establishment of incontinence-associated dermatitis rat models and assessment of the therapeutic effects of zinc oxide, painless skin protective film and silicone dressing.

Exp Ther Med 2021 Oct 26;22(4):1058. Epub 2021 Jul 26.

Medical Molecular Biology Laboratory, School of Medicine, Jinhua Polytechnic, Jinhua, Zhejiang 321007, P.R. China.

The aim of the present study was to construct incontinence-associated dermatitis (IAD) rat models and observe the therapeutic effects of zinc oxide, painless skin protective film and silicone dressing on IAD. A total of 54 rats were randomly divided into nine groups: i) Control group; ii) trypsin model group; iii) model + zinc oxide group; iv) model + painless skin protective film group; v) model + silicon dressing group; vi) synthetic urine combined with trypsin model group (joint model group); vii) joint model + zinc oxide group; viii) joint model + painless skin protective film group; and ix) joint model + silicone dressing group. A total of 4 days after applying the zinc oxide, protective film or silicon dressing intervention, IAD scores and pH values in skin tissues were examined. Read More

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October 2021

Microbiota Perturbation or Elimination Can Inhibit Normal Development and Elicit a Starvation-Like Response in an Omnivorous Model Invertebrate.

mSystems 2021 Aug 24;6(4):e0080221. Epub 2021 Aug 24.

Department of Evolution, Ecology and Organismal Biology, The Ohio State Universitygrid.261331.4, Columbus, Ohio, USA.

Omnivorous animals, including humans, harbor diverse, species-rich gut communities that impact their growth, development, and homeostasis. Model invertebrates are broadly accessible experimental platforms that enable linking specific species or species groups to host phenotypes, yet often their specialized diets and distinct gut microbiota make them less comparable to human and other mammalian and gut communities. The omnivorous cockroach Periplaneta americana harbors ∼4 × 10 bacterial genera within its digestive tract and is enriched with taxa commonly found in omnivorous mammals (i. Read More

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Nanostructured Phthalocyanine Assemblies with Efficient Synergistic Effect of Type I Photoreaction and Photothermal Action to Overcome Tumor Hypoxia in Photodynamic Therapy.

J Am Chem Soc 2021 Sep 23;143(34):13980-13989. Epub 2021 Aug 23.

College of Chemistry, State Key Laboratory of Photocatalysis on Energy and Environment, Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy, Fuzhou University, Fuzhou 350108, China.

Most photodynamic therapy (PDT) paradigms work through the highly O-dependent type II photoreaction to generate singlet oxygen (O). The hypoxic microenvironment of solid tumors severely hampers therapeutic outcomes. Here, we present a novel design that could transfer the photophysical and photochemical properties of traditional phthalocyanine-based photosensitizers from type II photoreaction to efficient type I photoreaction and vibrational relaxation-induced photothermal conversion. Read More

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September 2021

Protective effects of pretreatment with Fe, Cu, and Rb on phoxim poisoning in silkworm, Bombyx mori.

J Trace Elem Med Biol 2021 Aug 14;68:126844. Epub 2021 Aug 14.

College of Biotechnology and Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, 212018, PR China. Electronic address:

Background: Phoxim is a widely used organophosphorus pesticide in agriculture. People are paying more and more attention to its toxicity. At present, there is no appropriate way to solve the phoxim poisoning of silkworm, which severely affected the development of sericulture. Read More

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Antifungal compound, methyl hippurate from Bacillus velezensis CE 100 and its inhibitory effect on growth of Botrytis cinerea.

World J Microbiol Biotechnol 2021 Aug 22;37(9):159. Epub 2021 Aug 22.

Department of Agricultural and Biological Chemistry, Environmentally-Friendly Agricultural Research Center, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea.

Botrytis cinerea, the causal agent of gray mold is one of the major devastating fungal pathogens that occurs in strawberry cultivation and leads to massive losses. Due to the rapid emergence of resistant strains in recent years, an ecofriendly disease management strategy needs to be developed to control this aggressive pathogen. Bacillus velezensis CE 100 exhibited strong antagonistic activity with 53. Read More

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Melatonin Relieves Ozone Stress in Grape Leaves by Inhibiting Ethylene Biosynthesis.

Front Plant Sci 2021 28;12:702874. Epub 2021 Jul 28.

State Key Laboratory of Crop Biology, Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production in Shandong, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-an, China.

Ozone (O) stress severely affects the normal growth of grape ( L.) leaves. Melatonin (MT) plays a significant role in plant response to various abiotic stresses, but its role in O stress and related mechanisms are poorly understood. Read More

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Integrated ionomic and transcriptomic dissection reveals the core transporter genes responsive to varying cadmium abundances in allotetraploid rapeseed.

BMC Plant Biol 2021 Aug 13;21(1):372. Epub 2021 Aug 13.

School of Agricultural Sciences, Zhengzhou University, Zhengzhou, 450001, China.

Background: Oilseed rape (B. napus L.) has great potential for phytoremediation of cadmium (Cd)-polluted soils due to its large plant biomass production and strong metal accumulation. Read More

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Mitochondrial fusion mediated by mitofusin 1 regulates macrophage mycobactericidal activity by enhancing autophagy.

Infect Immun 2021 Aug 9:IAI0030621. Epub 2021 Aug 9.

Guangdong Provincial Key Laboratory of Regional Immunity and Diseases, Department of Pathogen Biology, Shenzhen University School of Medicine, Shenzhen, Guangdong 518060, China.

Mitochondria as a highly dynamic organelle continuously changes morphology and position during its life cycle. Mitochondrial dynamics including fission and fusion play a critical role in maintaining functional mitochondria for ATP production, which is directly linked to host defense against Mtb infection. However, how macrophages regulate mitochondrial dynamics during (Mtb) infection remains elusive. Read More

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Orientin mediates protection against MRSA-induced pneumonia by inhibiting Sortase A.

Virulence 2021 12;12(1):2149-2161

College of Animal Science, Jilin University, Changchun China.

Drug-resistant pathogenic () has severely threatened human health and arouses widespread concern. Sortase A (SrtA) is an essential virulence factor of , which is responsible for the covalent anchoring of a variety of virulence-related proteins to the cell wall. SrtA has always been regarded as an ideal pharmacological target against infections. Read More

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December 2021

The Role of Innate Immunity and Bioactive Lipid Mediators in COVID-19 and Influenza.

Front Physiol 2021 22;12:688946. Epub 2021 Jul 22.

Department of Internal Medicine II, Medical University of Innsbruck, Innsbruck, Austria.

In this review, we discuss spatiotemporal kinetics and inflammatory signatures of innate immune cells specifically found in response to SARS-CoV-2 compared to influenza virus infection. Importantly, we cover the current understanding on the mechanisms by which SARS-CoV-2 may fail to engage a coordinated type I response and instead may lead to exaggerated inflammation and death. This knowledge is central for the understanding of available data on specialized pro-resolving lipid mediators in severe SARS-CoV-2 infection pointing toward inhibited E-series resolvin synthesis in severe cases. Read More

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The Egr-1/miR-15a-5p/GPX4 axis regulates ferroptosis in acute myocardial infarction.

Eur J Pharmacol 2021 Oct 31;909:174403. Epub 2021 Jul 31.

Department of Pharmacology, Harbin Medical University-Daqing, No 39, Xin Yang Road, Daqing, Heilongjiang, 163319, China. Electronic address:

Acute myocardial infarction (AMI) is a type of cardiovascular diseases that severely threatens human being, but the mechanisms have not been thoroughly clarified. Here, we detected that microRNA-15a-5p (miR-15a-5p) was up-regulated in AMI. Knockdown of miR-15a-5p reduced cell mortality in hypoxic-treated myocardial cells. Read More

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October 2021

Mitochondria as a Potential Target for the Development of Prophylactic and Therapeutic Drugs against Schistosoma mansoni Infection.

Antimicrob Agents Chemother 2021 Sep 2;65(10):e0041821. Epub 2021 Aug 2.

Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan.

The emergence of parasites resistant to praziquantel, the only therapeutic agent, and its ineffectiveness as a prophylactic agent (inactive against the migratory/juvenile Schistosoma mansoni), make the development of new antischistosomal drugs urgent. The parasite's mitochondrion is an attractive target for drug development, because this organelle is essential for survival throughout the parasite's life cycle. We investigated the effects of 116 compounds against Schistosoma mansoni cercaria motility that have been reported to affect mitochondrion-related processes in other organisms. Read More

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September 2021

Nitric Oxide-Releasing Nanoparticles Are Similar to Efinaconazole in Their Capacity to Eradicate Biofilms.

Front Cell Infect Microbiol 2021 15;11:684150. Epub 2021 Jul 15.

Department of Medicine, Division of Infectious Diseases, Albert Einstein College of Medicine, Bronx, NY, United States.

Filamentous fungi such as and , the main causative agents of onychomycosis, have been recognized as biofilm-forming microorganisms. Nitric oxide-releasing nanoparticles (NO-np) are currently in development for the management of superficial and deep bacterial and fungal infections, with documented activity against biofilms. In this context, this work aimed to evaluate, for the first time, the anti- biofilm potential of NO-np using standard ATCC MYA-4438 and clinical BR1A strains and compare it to commonly used antifungal drugs including fluconazole, terbinafine and efinaconazole. Read More

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Computational and experimental characterization of isomers of escin-induced renal cytotoxicity by inhibiting heat shock proteins.

Eur J Pharmacol 2021 Oct 27;908:174372. Epub 2021 Jul 27.

State Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China. Electronic address:

Escin is a natural mixture of triterpene saponins, exhibits anti-oedematous properties and promotes venous drainage by oral administration or injection. Upon clinical application of escin, adverse kidney reactions have been reported and the nephrotoxic mechanism responsible for this reaction remains elusive. In the present study, four isomeric escins (β-form: escin Ia and escin Ib; α-form: isoescin Ia and isoescin Ib) were found severely decreasing the cell viability of human kidney (HK-2) cells. Read More

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October 2021

Impact of pulsed magnetic field treatment on enzymatic inactivation and quality of cloudy apple juice.

J Food Sci Technol 2021 Aug 22;58(8):2982-2991. Epub 2020 Sep 22.

School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013 Jiangsu China.

The effects of PMF (5-7 T, 5-30 pulses) on enzyme activity, pH, titratable acidity, soluble solids, color, ascorbic acid, total phenols and antioxidant activity (DPPH radical scavenging activity) of cloudy apple juice were evaluated. PMF inhibited activities of polyphenoloxidase (PPO), peroxidase (POD) and pectinmethylesterase (PME), but PPO was more sensitive to PMF than POD and PME. At the intensity of 6 T with 15 pulses, PPO and POD both exhibited the lowest residual activity (53. Read More

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Huaier Inhibits Proliferation, Migration, and Invasion of Cutaneous Squamous Cell Carcinoma Cells by Inhibiting the Methylation Levels of CDKN2A and TP53.

Integr Cancer Ther 2021 Jan-Dec;20:15347354211031646

Department of Dermatology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.

Cutaneous squamous cell carcinoma (CSCC) is a malignant tumor that originates from keratinocytes in the epidermis or appendage. Traditional Chinese medicine Huaier has anti-tumor activity in various malignancies. Little is known about the role of Huaier in CSCC. Read More

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Reduced DAPK1 Expression Promotes Stem Cell-Like Characteristics of Prostate Cancer Cells by Activating ZEB1 via Hippo/YAP Signaling Pathway.

Stem Cells Dev 2021 Sep 17;30(18):934-945. Epub 2021 Aug 17.

Department of Urological Surgery, The Affiliated Hospital of Nantong University, Nantong City, Jiangsu Province, China.

Prostate cancer (PCa) is a malignant tumor that originates in the male genitourinary system. Downregulation of death-associated protein kinase 1 (DAPK1) is closely related to PCa. Little is known about the functional role of DAPK1 in regulating cancer stem cell (CSC)-like characteristics of PCa cells, and we have conducted research on this topic. Read More

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September 2021

Time- and dose-dependent allelopathic effects and mechanisms of kaempferol on toxigenic Microcystis growth.

Ecotoxicol Environ Saf 2021 Oct 17;222:112508. Epub 2021 Jul 17.

College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China; Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Beijing 100193, China. Electronic address:

This study determined time-dependent IC and confirmed 3.5 mg/L as IC value for kaempferol inhibiting toxigenic Microcystis growth, based on which algicidal effects and mechanisms against toxigenic Microcystis exposed to various kaempferol doses (0.5-2 × IC) were explored along 14 day-test. Read More

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October 2021

Taurochenodeoxycholic acid inhibits the proliferation and invasion of gastric cancer and induces its apoptosis.

J Food Biochem 2021 Jul 18:e13866. Epub 2021 Jul 18.

Department of Spleen and Stomach, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China.

Taurochenodeoxycholic acid (TCDCA) is the principal ingredient of Compound Shougong Powder. Despite traditional Chinese medicine (TCM) research demonstrates that Compound Shougong Powder can restrict tumor growth, whether TCDCA exerts a role in suppressing cancer as the major ingredient of Compound Shougong Powder remains unknown. This study aims to clarify the regulatory mechanism of TCDCA on gastric cancer. Read More

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Danshensu alleviates pseudo-typed SARS-CoV-2 induced mouse acute lung inflammation.

Acta Pharmacol Sin 2021 Jul 15. Epub 2021 Jul 15.

Guangdong Provincial Key Laboratory of New Drug Screening, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can induce acute inflammatory response like acute lung inflammation (ALI) or acute respiratory distress syndrome, leading to severe progression and mortality. Therapeutics for treatment of SARS-CoV-2-triggered respiratory inflammation are urgent to be discovered. Our previous study shows that Salvianolic acid C potently inhibits SARS-CoV-2 infection. Read More

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TGF-β blockade-improved chemo-immunotherapy with pH/ROS cascade-responsive micelle via tumor microenvironment remodeling.

Biomaterials 2021 09 6;276:121010. Epub 2021 Jul 6.

School of Life Science, Northwestern Polytechnical University, Xi'an, 710072, China. Electronic address:

Immunosuppressive tumor microenvironment and low delivery efficiency severely impede the tumor chemotherapy effect. To address this issue, we develop a pH/ROS cascade-responsive prodrug micelle to deliver siTGF-β with size-shrinkage and charge-reversal property, leading to synergistical tumor microenvironment remodeling. The nanosystem highly improved endocytosis efficiency and tumor penetration depth through charge reversal and size reduction upon exposure to weakly acidic tumor microenvironment. Read More

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September 2021