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Evaluation of the Frequency of RETN c.62G>A and RETN c.-180C>G Polymorphisms in the Resistin Coding Gene in Girls with Anorexia Nervosa.

Endokrynol Pol 2021 Jul 22. Epub 2021 Jul 22.

Department of Adult Psychiatry, University of Medical Sciences in Poznań, Poland.

Introduction: Anorexia nervosa (AN) is a serious psychosomatic syndrome, classified as an eating disorder. AN patients strive to lose weight below normal limits defined for a specific age and height, achieving their goal even at the expense of extreme emaciation. AN has a multifactorial etiology. Read More

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Evaluation of the Frequency of ADIPOQ c.45 T>G and ADIPOQ c.276 G>T Polymorphisms in the Adiponectin Coding Gene in Girls with Anorexia Nervosa.

Endokrynol Pol 2021 Jul 22. Epub 2021 Jul 22.

Department of Adult Psychiatry, University of Medical Sciences in Poznań, Poland.

Introduction: Anorexia nervosa (AN) is a serious chronic psychosomatic disorder, the essence of which are attempts to obtain a slim silhouette by deliberate weight loss (restrictive diet, strenuous physical exercise, provoking vomiting). The etiology of this disorder is multifactorial. Genetic factors which influence the predisposition to AN have been searched for. Read More

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Signal Separated Quantification of γ-Hydroxybutyrate With Liquid Chromatography-Tandem Mass Spectrometry in Human Urine and Serum as an Improvement of the Analyte Adduct Ion Based Quantification.

J Anal Toxicol 2021 Jul 20. Epub 2021 Jul 20.

Institute of Legal Medicine, Hannover Medical School (MHH), Carl-Neuberg-Str. 1, D-30625 Hannover, Germany.

The aim of the work was the development and validation of an LC-MS/MS γ-hydroxybutyrate (GHB) quantification method in urine and human serum by the use of the analyte adduct ion formation strategy. A combined detection with a conventional precursor ion in the negative electrospray mode and additionally GHB adduct ions with both sodium acetate and lithium acetate was in focus. Therefore GHB quantification based on separated MS/MS signals. Read More

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Assessment of intrahepatic cholestasis in pregnancy and the effect of disease severity on transient tachypnea in the newborn in uncomplicated fetuses.

J Perinat Med 2021 Jul 20. Epub 2021 Jul 20.

Department of Obstetrics and Gynecology, Kayseri City Hospital, Kayseri, Turkey.

Objectives: Considering the effects of bile-acid levels on fetal lungs and pulmonary surfactants, we hypothesized that in the presence of intrahepatic pregnancy cholestasis (ICP), poor neonatal respiratory problems are observed in relation to the severity of the disease. Delivery timing with the presence of ICP is scheduled during late-preterm and early term gestational weeks. The aim of this study was to assess ICP and disease severity effects on transient tachypnea of the newborn (TTN) in uncomplicated fetuses. Read More

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Development and validation of an LC-MS/MS assay for the quantification of allopregnanolone and its progesterone-derived isomers, precursors, and cortisol/cortisone in pregnancy.

Anal Bioanal Chem 2021 Jul 19. Epub 2021 Jul 19.

iC42 Clinical Research & Development, Department of Anesthesiology, University of Colorado Anschutz Medical Campus, Bioscience 2, Suite 200, 12705 East Montview Blvd, Aurora, CO, 80045, USA.

Neuroactive steroids are potent neuromodulators that play a critical role in both maternal and fetal health during pregnancy. These stress-responsive compounds are reportedly low in women with perinatal depression and may be associated with poor pregnancy outcomes in animal models. Chronic stress is a risk factor for adverse birth outcomes. Read More

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Hypocholesterolemic Effect of Blackcurrant () Extract in Healthy Female Subjects: A Pilot Study.

Molecules 2021 Jul 4;26(13). Epub 2021 Jul 4.

Department of Nursing Sciences, Hirosaki University Graduate School of Health Sciences, 66-1 Hon-cho, Hirosaki 036-8564, Japan.

Blackcurrant extract (BCE) ameliorates dyslipidemia in menopausal model animals and in elderly women at a risk of dyslipidemia. However, it is unknown whether the daily intake of BCE can prevent lipid abnormalities in healthy individuals. Lipids are essential for the body, but they also cause arteriosclerosis. Read More

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Total thiol can contribute to differentiating prostate cancer from BPH: Prostate Thiol Index as a new player.

Andrologia 2021 Jul 16:e14190. Epub 2021 Jul 16.

Department of Medical Biochemistry, Yıldırım Beyazıt University Faculty of Medicine, Ankara, Turkey.

To assess the distinctiveness of serum native thiol (NT), total thiol (TT) and disulfide (SS) levels in PCa patients, we created a new parameter, prostate thiol index (PTI) [tPSA (TTxPVxAge) ]. We determined the performance of the PTI on PCa diagnosis. A total of 107 male patients (PCa:65; BPH:42) who were separated according to their Gleason scores, ISUP grades and EAU risk groups and 20 healthy subjects were included. Read More

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Salty Biofluidic Sample Clean-Up and Preconcentration with a Paper-Based Ion Concentration Polarization Interface.

Anal Chem 2021 Jul 16. Epub 2021 Jul 16.

Research Center for Analytical Sciences, Chemistry Department, College of Sciences, Northeastern University, Shenyang 110819, China.

Charged species from complex media could be separated and enriched taking advantage of ion concentration polarization (ICP) effect; thus, ICP can be used for sample purification and improvement of detection sensitivity. In this paper, a novel and reliable ICP interface was established on a paper-based analytical device (PAD) by using ion exchange membrane, and electrokinetic stacking of target analytes from salty media was successfully demonstrated. Steady ICP effect was well observed in aqueous solution with up to 400 mM NaCl as shown by a fluorescent probe, which makes it possible to directly process salty physiological samples such as blood and urine with this type of PAD. Read More

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Evaluation of the Risk of Clinical Deterioration among Inpatients with COVID-19.

Adv Virol 2021 25;2021:6689669. Epub 2021 Jun 25.

Intensive Therapy and Dermatology Hospital Monte Sinai, Juiz de Fora 36033-318, Brazil.

This study aims to assess the risk of severe forms of COVID-19, based on clinical, laboratory, and imaging markers in patients initially admitted to the ward. This is a retrospective observational study, with data from electronic medical records of inpatients, with laboratory confirmation of COVID-19, between March and September 2020, in a hospital from Juiz de Fora-MG, Brazil. Participants ( = 74) were separated into two groups by clinical evolution: those who remained in the ward and those who progressed to the ICU. Read More

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The Preventive Role of () in Immune and Oxidative Insults in a Stress-induced Rat Model.

J Vet Res 2021 Jun 8;65(2):193-200. Epub 2021 Jun 8.

Department of Physiology, Faculty of Veterinary Medicine, Bursa Uludağ University, 16059 Bursa, Turkey.

Introduction: There is a balance between oxidative stress, antioxidant capacity and immune response. Their roles in physiological and behavioural mechanisms are important for the maintenance of the organism's internal equilibrium. This study aimed to evaluate the antioxidant effects of the exogenous alga in a stress-induced rat model, and to describe its possible mechanism of action. Read More

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Exploring the malnutrition status and impact of total parenteral nutrition on the outcome of patients with advanced stage ovarian cancer.

BMC Cancer 2021 Jul 10;21(1):799. Epub 2021 Jul 10.

School of Nursing, Shanxi Medical University, Taiyuan, China.

Background: Ovarian cancer is a common cancer type in women and is often associated with onset of malnutrition. Total parenteral nutrition (TPN) is a nutritional intervention method that has been reported to have controversial effect on cancer patients. In the present retrospective study, we sought to explore the prevalence of malnutrition assessed by the Nutritional Risk Index (NRI) and its association with survival in advanced stage ovarian cancer patients. Read More

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Integration of FGF21 Signaling and Metabolomics in High-Fat Diet-Induced Obesity.

J Proteome Res 2021 Jul 8. Epub 2021 Jul 8.

Institute of Metabonomics & Medical NMR, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China.

Sex differences in obesity have been well established, but the metabolic mechanism underlying these differences remains unclear. In the present study, we determined the expression levels of endogenous fibroblast growth factor 21 (FGF21) and its related receptors in male and female mice that were fed a high-fat diet (HFD) for 12 weeks. We also analyzed the metabolic changes in serum and livers using a nuclear magnetic resonance-based metabolomics approach. Read More

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Evaluation of local and circulating osteopontin in malignant and benign primary bone tumors.

J Bone Oncol 2021 Aug 19;29:100377. Epub 2021 Jun 19.

Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

Purpose: The development of novel and efficient biomarkers for primary bone cancers is of grave importance.

Methods: The expression pattern of osteopontin (OPN) was investigated in the 153 patients with benign (n = 72) and malignant (n = 81) primary bone cancers. Both local and circulating OPN mRNA expression levels and their protein concentration in serum and tumor site were assessed using real-time qRT-PCR, ELISA, and immunohistochemistry techniques, respectively. Read More

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Surface-enhanced Raman scattering holography chip for rapid, sensitive and multiplexed detection of human breast cancer-associated MicroRNAs in clinical samples.

Biosens Bioelectron 2021 Oct 30;190:113470. Epub 2021 Jun 30.

Laboratory of Nanoscale Biochemical Analysis, Institute of Functional Nano & Soft Materials (FUNSOM), Soochow University, Suzhou, Jiangsu, 215123, China. Electronic address:

MicroRNAs (miRNAs) are promising biomarkers for the early diagnosis of breast cancer. Yet, simultaneous achievement of rapid, sensitive and accurate detection of diverse miRNAs in clinical samples is still challenging due to the low abundance of miRNAs and the complex procedures of RNA extraction and separation. Herein, we develop an innovative three-dimensional (3D) surface-enhanced Raman scattering (SERS) holography sensing strategy for rapid, sensitive and multiplexed detection of human breast cancer-associated miRNAs. Read More

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October 2021

Effects of inorganic copper injection in beef cows at late gestation on fetal and postnatal growth, hematology and immune function of their progeny.

Res Vet Sci 2021 Jun 26;139:11-17. Epub 2021 Jun 26.

Estación Experimental Agropecuaria (EEA) Cuenca del Salado, Instituto Nacional de Tecnología Agropecuaria (INTA), Belgrano 416, Rauch 7203, Buenos Aires, Argentina.

To determine if Cu injection during late gestation can affect fetal and postnatal growth, hematology and immune function of progeny, 70 multiparous pregnant Angus cows, at 219 ± 15 d of gestation, were ranked by BW and BCS and randomly assigned to one of two treatments: Cu + (n = 35) in total 160 mg of Cu were administered subcutaneously in two moments (80 mg per moment) at 64 ± 15 d and 54 ± 15 d prepartum; and Cu- (n = 35), in total of 16 ml of sterile NaCl solution (9 g / l) were administered subcutaneously in two moments (8 ml per moment) at 64 ± 15 d and 54 ± 15 d prepartum. Calves from both treatments were weaned at 260 ± 15 d of age, male calves were separated from female calves and stockered on natural pastures until 690 ± 15 d of age, then placed into a feedlot for 104 d before slaughter. At the beginning of the experiment, cows Cu serum concentration was similar (P = 0. Read More

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Isolation and Purification of Low Molecular Weight Proteins from Culture Filtrate of Mycobacterium Tuberculosis Strain C.

Arch Razi Inst 2021 Jul 1;76(2):273-281. Epub 2021 Jul 1.

Department of Microbiology, Arak University of Medical Sciences, Arak, Iran.

In the last couple of years, a number of new and rapid tests for the diagnosis of Tuberculosis (TB) have been developed based on the low molecular weight antigens from Mycobacterium tuberculosis (Mtb) culture supernatant. This study aimed to isolate and purify low molecular weight antigens secreted by Mtb strain C for diagnostic purpose. The secretory proteins from culture filtrate of Mtb were extracted using ammonium sulphate precipitations and sephadex-G50 gel chromatography. Read More

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Ultracentrifugal separation, characterization, and functional study of extracellular vesicles derived from serum-free cell culture.

STAR Protoc 2021 Sep 23;2(3):100625. Epub 2021 Jun 23.

Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma 630-0192, Japan.

Extracellular vesicles (EVs) play important roles in extracellular trafficking and signaling. Here, we separate EVs by differential centrifugation. EVs separated by this approach are called large EVs (l-EVs) and small EVs (s-EVs), reflecting particle size, which sediment based on different ultracentrifugation forces. Read More

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September 2021

Protective Effect of Red Okra ( (L.) Moench) Pods against Sodium Nitrite-Induced Liver Injury in Mice.

Vet Med Int 2021 16;2021:6647800. Epub 2021 Jun 16.

Department of Biology, Faculty of Science and Technology, Airlangga University, Surabaya 60115, Indonesia.

Vegetables, drinking water, and preserved meats may contain sodium nitrite (NaNO), which causes liver disease by inducing oxidative stress. Phytochemicals are highly recommended as an alternative to synthetic drugs and affordable medicines to treat liver disease because they have fewer or no side effects. Therefore, this study aims to determine the antioxidant and hepatoprotective potential of red okra fruit ethanol extract against NaNO-induced liver damage. Read More

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Glucocorticoid Abnormalities in Female Rats Exposed to a Predator-Based Psychosocial Stress Model of PTSD.

Front Behav Neurosci 2021 18;15:675206. Epub 2021 Jun 18.

Department of Pharmaceutical Sciences, Marshall University School of Pharmacy, Huntington, WV, United States.

People with post-traumatic stress disorder (PTSD) exhibit heightened anxiety and enhanced negative feedback of the hypothalamus-pituitary-adrenal (HPA) axis. We previously reported that male rats exposed to a predator-based psychosocial stress model of PTSD exhibited comparable changes in anxiety-like behavior and HPA axis activity, including lower baseline levels of corticosterone and a greater suppression of corticosterone after dexamethasone administration. Here, we assessed whether we would observe similar effects in female rats exposed to this model. Read More

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Surface-enhanced Raman spectroscopy for comparison of serum samples of typhoid and tuberculosis patients of different stages.

Photodiagnosis Photodyn Ther 2021 Jul 1;35:102426. Epub 2021 Jul 1.

Department of Chemistry, University of Agriculture Faisalabad, Faisalabad 38040, Pakistan.

Background: Surface-enhanced Raman spectroscopy (SERS) is a reliable tool for the identification and differentiation of two different human pathological conditions sharing the same symptomology, typhoid and tuberculosis (TB).

Objectives: To explore the potential of surface-enhanced Raman spectroscopy for differentiation of two different diseases showing the same symptoms and analysis by principal component analysis (PCA) and partial least square discriminate analysis (PLS-DA).

Methods: Serum samples of clinically diagnosed typhoid and tuberculosis infected individuals were analyzed and differentiated by SERS using silver nanoparticles (Ag NPs) as a SERS substrate. Read More

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Preliminary characterization of pathogen-detection activities of serum antibodies from the banded houndshark Triakis scyllium.

Dev Comp Immunol 2021 Jun 29;124:104186. Epub 2021 Jun 29.

Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo, 108-8477, Japan.

Antibodies of cartilaginous fish are of scientific interest due to their phylogenetic position. In the present study, we developed antiserum against IgM of the banded houndshark, Triakis scyllium, and characterized binding activity of the IgM against fish pathogenic bacteria. Pentameric and monomeric IgM antibodies were separated by gel filtration chromatography using high performance liquid chromatography and SDS-PAGE. Read More

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[Analysis of three antipyretic analgesic drugs by open-tubular capillary electrochromatography].

Se Pu 2020 Sep;38(9):1107-1114

Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

The advantages of capillary electrophoresis, such as small sample consumption, high separation efficiency, and multiple separation modes, have been known for decades. However, exploring unique capillary electrophoresis techniques for the analysis of fluid drugs in living bio-systems remains an important and urgent task. Owing to the similar structures and mass-to-charge ratios of antipyretic analgesic drugs, efficient baseline separation of these analytes by capillary zone electrophoresis method cannot be easily achieved. Read More

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September 2020

[Application of poly(2-methyl-2-oxazoline) in protein separation by capillary electrophoresis].

Se Pu 2020 Sep;38(9):1022-1027

Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, China.

Capillary electrophoresis (CE), a commonly used liquid-phase separation technology, has many advantages such as high analysis speed, high separation efficiency, and low sample consumption. Hence, CE has gained popularity in food analysis, medical clinical diagnosis, environmental monitoring, and biological sample separation, especially in the field of protein separation and analysis. However, the fused silica capillaries that are commonly used in CE easily adsorb proteins, resulting in unstable electroosmotic flow and poor reproducibility of the separation results. Read More

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September 2020

[Determination of four bisphenol environmental hormone residues in infant serum by liquid chromatography-tandem mass spectrometry].

Se Pu 2020 Dec;38(12):1381-1387

College of Chemistry and Food Engineering, Changsha University of Science and Technology, Changsha 410014, China.

Bisphenols are important industrial raw materials that are widely used to produce plastic bottles (feeding bottles), infant cups, and food and beverage (milk powder) cans. Because of the estrogen-like effect of bisphenols, even low-dose intake of these compounds by trace migration affects normal hormone levels in the human body. Therefore, it is imperative to develop a rapid, accurate, and highly sensitive method for the determination of bisphenols in serum. Read More

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December 2020

The effects on bacterial translocation and tissue damage of selenium treatment in an experimental intestina ischaemia-reperfusion model.

Ulus Travma Acil Cerrahi Derg 2021 Jul;27(4):389-394

Department of Orthopedics and Traumatology, University of Health Sciences, Baltalimani Bone Diseases Training and Research Center, İstanbul-Turkey.

Background: The free oxygen radicals formed with reperfusion following intestinal ischaemia are extremely toxic for the cells. Glutathione peroxidase, an important enzyme that prevents the formation of reactive oxygen species, requires selenium as a co-factor. This study aims to demonstrate the effects of selenium administration on reducing ischaemia-reperfusion damage. Read More

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Imbalanced dietary intake alters the colonic microbial profile in growing rats.

PLoS One 2021 30;16(6):e0253959. Epub 2021 Jun 30.

Convergence Research Center, Sahmyook University, Seoul, Korea.

An imbalanced dietary intake is associated with alteration of intestinal ecosystem. We investigated the impact of imbalanced diets on colonic microbiota, concentrations of short chain fatty acid in colonic digesta and serum immunoglobulins (Igs) of growing rats. Compared to the control diet, consuming diets high in fat, sucrose, or processed meat, or low in iron, increased the abundance of the pathogenic bacteria such as Clostridium, Escherichia coli, and Salmonella species, and decreased the beneficial bacteria, like Bifidobacteria, Lactobacillus, Akkermansia, Phascolarctobacterium, Alistipes, and butyrate producing species of bacteria in the colon of growing rats. Read More

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Microwave ablation as an efficient therapy for primary hyperparathyroidism: Efficacy and predictors of treatment success.

Int J Clin Pract 2021 Jun 29:e14580. Epub 2021 Jun 29.

Division of Endocrinology and Metabolism, Department of Internal Medicine, Izmir Katip Celebi University, Ataturk Training and Research Hospital, Izmir, Turkey.

Background: Microwave ablation (MWA) has great potential for the treatment of primary hyperparathyroidism, but as predictors and therapeutic efficiency have not been fully clarified, further investigations are required.

Aim: The purpose of this study was to explore the predictors of MWA efficacy in patients with primary hyperparathyroidism (PHPT) induced by parathyroid adenoma.

Methods: The study included patients with PHPT treated with MWA separated into two groups as response and no response group, according to the efficacy of the treatment. Read More

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The Systematic Effect of Mesenchymal Stem Cell Therapy in Critical COVID-19 Patients: A Prospective Double Controlled Trial.

Cell Transplant 2021 Jan-Dec;30:9636897211024942

Liv Hospital, Center for Regenerative Medicine and Stem Cell Manufacturing (Liv Med Cell), Istanbul, Turkey.

The aim of this clinical trial was to control the cytokine storm by administering mesenchymal stem cells (MSCs) to critically-ill COVID-19 patients, to evaluate the healing effect, and to systematically investigate how the treatment works. Patients with moderate and critical COVID-19 clinical manifestations were separated as Group 1 (moderate cases, = 10, treated conventionally), Group 2 (critical cases, = 10, treated conventionally), and Group 3 (critical cases, = 10, treated conventionally plus MSCs transplantation therapy of three consecutive doses on treatment days 0, 3, and 6, (as 3 × 10 cells/kg, intravenously). The treatment mechanism of action was investigated with evaluation markers of the cytokine storm, via biochemical parameters, levels of proinflammatory and anti-inflammatory cytokines, analyses of tissue regeneration via the levels of growth factors, apoptosis markers, chemokines, matrix metalloproteinases, and granzyme-B, and by the assessment of the immunomodulatory effects via total oxidant/antioxidant status markers and the levels of lymphocyte subsets. Read More

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Separated serum was used for the analysisCombined Analysis of Anti SARS-CoV-2 IgG and IgM Responses in COVID19 Patients in India.

Indian J Clin Biochem 2021 Jun 18:1-7. Epub 2021 Jun 18.

Department of Biochemistry, Post Graduate Institute of Medical Education and Research, Chandigarh, India.

Coronavirus disease 2019 (COVID-19) caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a global health problem, India being the second most affected country. The kinetics of antibody response to SARS-CoV-2 in Indian population is not studied yet. To understand serological response in relation to age, gender, time period and severity of disease, Roche Elecsys anti-SARS-CoV-2 test was used which analysed both IgM and IgG. Read More

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Salvia plebeia R. Br. polysaccharides (SPP) against RSV (respiratory syncytial virus) infection: Antiviral effect and mechanisms of action.

Biomed Pharmacother 2021 Jun 24;141:111843. Epub 2021 Jun 24.

Shandong University of Traditional Chinese Medicine, Ji'nan 250355, China. Electronic address:

Objective: To investigate the antiviral effect of Salvia plebeia R. Br. polysaccharides (SPP) against RSV and underlying mechanisms. Read More

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