137,073 results match your criteria serum samples

Epitope-imprinted polydopamine electrochemical sensor for ovalbumin detection.

Bioelectrochemistry 2021 Mar 26;140:107805. Epub 2021 Mar 26.

Department of Food, Agriculture and Bioresources, School of Environment, Resources and Development, Asian Institute of Technology, 58 Moo 9, Km. 42, Paholyothin Highway, Klong Luang, Pathumthani 12120, Thailand. Electronic address:

A novel, sensitive and selective electrochemical sensor based on epitope-imprinted polydopamine (PDA) was developed for ovalbumin (OVA) detection. Molecularly imprinted polydopamine was synthesized on an AuNP-coated screen-printed carbon electrode (SPCE) via electropolymerization in the presence of OVA IgE-binding epitope as the template. Key process parameters including template concentration, electropolymerization cycle, pH, time required for template removal and rebinding were optimized. Read More

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Effects of low-carbohydrate diet and ketogenic diet on glucose and lipid metabolism in type 2 diabetic mice.

Nutrition 2021 Mar 4;89:111230. Epub 2021 Mar 4.

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, China. Electronic address:

Objective: With the prevalence of diabetes worldwide, it is urgent to find a suitable treatment. Recently, the ketogenic diet has shown beneficial effects in reducing blood glucose, but some concerns have been raised about its probable side effects, such as hyperlipidemia and hepatic steatosis. Because a low-carbohydrate diet replaces part of the fat with carbohydrates on the basis of the ketogenic diet, we would like to know whether it does better in treating type 2 diabetes. Read More

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Oligonucleotide sensor based on magnetic capture and photoligation of upconverting nanoparticles in solid surfaces.

J Colloid Interface Sci 2021 Mar 11;596:64-74. Epub 2021 Mar 11.

Department of Optics, Complutense University of Madrid, E-28037 Madrid, Spain. Electronic address:

In this work, we present a luminescence platform that can be used as point of care system for determining the presence and concentration of specific oligonucleotide sequences. This sensor exhibited a limit of detection as low as 50 fM by means of: (i) the use of single-stranded DNA (ssDNA) functionalized magnetic microparticles that captured and concentrated ssDNA-upconverting nanoparticles (ssDNA-UCNPs) on a solid support, when the target sequence (miR-21-5p DNA-analogue) was in the sample, and (ii) a photoligation reaction that covalently linked the ssDNA-UCNPs and the ssDNA magnetic microparticles, allowing stringent washes. The presented sensor showed a similar limit of detection when the assays were conducted in samples containing total miRNA extracted from human serum, demonstrating its suitability for detecting small specific oligonucleotide sequences under real-like conditions. Read More

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Inflammatory markers in autoimmunity induced by checkpoint inhibitors.

J Cancer Res Clin Oncol 2021 Apr 10. Epub 2021 Apr 10.

Department of Dermatology, Universitätsklinikum Erlangen, Ulmenweg 18, 91054, Erlangen, Germany.

Purpose: Immune checkpoint inhibitors (ICI) are highly effective in several cancer entities, but also invoke a variety of immune-related adverse events (irAE). These are mostly reversible, but can be life-threatening or even fatal. Currently, the pathogenesis is not fully understood, but crucial for effective treatment. Read More

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The circulating soluble form of the CD40 costimulatory immune checkpoint receptor and liver metastasis risk in rectal cancer.

Br J Cancer 2021 Apr 9. Epub 2021 Apr 9.

Department of Oncology, Akershus University Hospital, Lørenskog, Norway.

Background: In colorectal cancer, the inflamed tumour microenvironment with its angiogenic activities is immune- tolerant and incites progression to liver metastasis. We hypothesised that angiogenic and inflammatory factors in serum samples from patients with non-metastatic rectal cancer could inform on liver metastasis risk.

Methods: We measured 84 angiogenic and inflammatory markers in serum sampled at the time of diagnosis within the population-based cohort of 122 stage I-III patients. Read More

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Serum-derived exosomal PD-L1 expression to predict anti-PD-1 response and in patients with non-small cell lung cancer.

Sci Rep 2021 Apr 9;11(1):7830. Epub 2021 Apr 9.

Department of Surgery, Tokyo Medical University Hospital, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo, 160-0023, Japan.

PD-L1 expression is the most useful predictive biomarker for immunotherapy efficacy on non-small cell lung cancer (NSCLC), and CD8+ tumor-infiltrating lymphocytes (CD8+ TILs) play an essential role in the clinical activity of immunotherapy. PD-L1 is found on the exosome's surface, and PD-L1 expressing exosomes can inhibit antitumor immune responses. This study aimed to analyze tumor PD-L1 expression, serum exosomal PD-L1, and CD8+ TILs to investigate anti-PD-1 response and clinicopathological outcomes in NSCLC. Read More

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Systemic conbercept pharmacokinetics and VEGF pharmacodynamics following intravitreal injections of conbercept in patients with retinopathy of prematurity.

Br J Ophthalmol 2021 Apr 9. Epub 2021 Apr 9.

Department of Ophthalmology, Peking University People's Hospital, Eye diseases and Optometry Institute, Beijing Key Laboratory of Diagnosis and Therapy of Retinal and Choroid Diseases, Clinical Centre of Optometry, College of Optometry, Peking University Health science center, Beijing, China

Background: Data on serum vascular endothelial growth factor (VEGF) and drug levels in patients with retinopathy of prematurity (ROP) following intravitreal injections of conbercept (IVC) are lacking.

Methods: Multicentre, prospective, non-randomised study of patients with aggressive posterior retinopathy of prematurity (APROP) or type 1 ROP who had not received other treatment. All infants received therapy in both eyes plus intravitreal IVC 0. Read More

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The impact of different preanalytical methods related to CA 15-3 determination in frozen human blood samples: a systematic review.

Syst Rev 2021 Apr 9;10(1):102. Epub 2021 Apr 9.

IRCCS SDN, Via E. Gianturco, 80143, Naples, Italy.

Background: The determination of CA 15-3 is useful for monitoring breast cancer patients. Several retrospective studies determined CA 15-3 levels in frozen samples to evaluate the sensitivity and specificity of novel biomarkers in relation to breast cancer; however, freeze-thaw cycles, as well as preanalytical variables before sample storage, are not always reported. Here, we analyzed the current scientific literature to identify possible critical aspects related to CA 15-3 determination in frozen-stored human serum/plasma samples. Read More

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Determination of serum 25-hydroxyvitamin D status among population in southern China by a high accuracy LC-MS/MS method traced to reference measurement procedure.

Nutr Metab (Lond) 2020 Jan 17;17(1). Epub 2020 Jan 17.

Department of Laboratory Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.

Objective: We aimed to describe the 25-hydroxyvitamin D (25(OH)D) status of southern Chinese individuals by a high-accuracy liquid chromatography tandem mass spectrometry (LC-MS/MS) method which can trace to reference measurement procedure.

Materials And Methods: From January 2018 to June 2019, a total of 4775 southern Chinese individuals were evaluated in our study. The serum levels of parathyroid hormone (PTH) were detected simultaneously in 162 cases. Read More

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January 2020

MOF@MnO2 nanocomposites prepared by in situ method and recyclable cholesterol oxidase-inorganic hybrid nanoflowers for cholesterol determination.

Nanotechnology 2021 Apr 9. Epub 2021 Apr 9.

Qufu Normal University, Qufu, Shandong, CHINA.

In this work, through in situ growth of MnO2 nanosheets on the surface of terbium metal-organic frameworks (Tb-MOFs), the MOF@MnO2 nanocomposites are prepared and the fluorescence of Tb-MOFs is quenched significantly by MnO2. Additionally, the hybrid nanoflowers are self-assembled by cholesterol oxidase (ChOx) and copper phosphate (Cu3(PO4)2•3H2O). Then a new strategy for cholesterol determination is developed based on MOF@MnO2 nanocomposites and hybrid nanoflowers. Read More

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Procalcitonin and C-reactive protein predict infection in hematopoietic stem cell transplantation patients.

Leuk Res 2021 Apr 2;105:106574. Epub 2021 Apr 2.

Department of clinical laboratory, Peking University People's Hospital, Beijing, PR China. Electronic address:

Introduction: Procalcitonin (PCT) and C-reactive protein (CRP) are known inflammatory markers of severe infection; however, their ability to differentiate between infections of different origins is not clear yet. In this study, we evaluated PCT and CRP as markers of infection in hematopoietic stem cell transplantation (HSCT) patients.

Methods: Blood samples were collected to determine serum concentrations of PCT, CRP, d-Dimer, and to perform blood culture analysis. Read More

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Serum lipid profiling analysis and potential marker discovery for ovarian cancer based on liquid chromatography-Mass spectrometry.

J Pharm Biomed Anal 2021 Mar 26;199:114048. Epub 2021 Mar 26.

CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.

Low early diagnosis rate and unclear pathogenesis are the primary reasons for the high mortality of epithelial ovarian cancer (EOC). Lipidomics is a powerful tool for marker discovery and mechanism explanation. Hence, a ultra high-performance liquid chromatography-mass spectrometry based non-targeted lipidomics analysis was performed to acquire lipid profiling of 153 serum samples including healthy control (HC, n = 50), benign ovarian tumor (BOT, n = 41), and EOC (n = 62) to reveal lipid disturbance, then differential lipids were verified in another sample set including 187 sera. Read More

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The effect of Gestational Diabetes Mellitus on the fetal compartment.

J Reprod Immunol 2021 Mar 24;145:103314. Epub 2021 Mar 24.

Departamento de Obstetrícia, Escola Paulista de Medicina, Universidade Federal de São Paulo (EPM-UNIFESP), Rua Pedro de Toledo 669, 9 andar - 04939032, São Paulo, SP, Brazil. Electronic address:

In indicated preterm births such a Gestational Diabetes Mellitus (GDM), little is known about the role of the amnion membranes. Investigating the role of amnion membrane inflammation in response GDM may suggest novel pathophysiologic mechanisms. We hypothesize that increased GDM inflammatory mediators may weaken the amnion membrane predisposing them to infection. Read More

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Natural variability in surface antigen and reverse transcriptase domain of hepatitis B virus in treatment-naïve chronic HBV-infected Egyptian patients.

Virus Res 2021 Apr 6:198422. Epub 2021 Apr 6.

Microbiology and Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt. Electronic address:

Hepatitis B virus (HBV) infection is a serious health problem not only in Egypt, but also worldwide. We collected 57 serum samples from treatment-naïve chronic HBV-infected Egyptians. The DNA encoding hepatitis surface antigen (HBsAg) and reverse transcriptase (RT) domain was partially sequenced. Read More

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Prediction of parasitological cure in children infected with Trypanosoma cruzi using a novel multiplex serological approach: an observational, retrospective cohort study.

Lancet Infect Dis 2021 Apr 6. Epub 2021 Apr 6.

Parasitology Service, Hospital de Niños "Ricardo Gutierrez" and Instituto Multidisciplinario de Investigacion en Patologias Pediatricas (IMIPP)-CONICET-GCBA, Buenos Aires, Argentina.

Background: Assessment of therapeutic response with standard serological diagnostic assays in patients with chronic Chagas disease is a major challenge due to the long persistence of parasite-specific antibodies. The current consensus for parasitological cure is to monitor conversion from positive to negative Trypanosoma cruzi serology (seroreversion). However, because of robust humoral immune response, seroreversion by standard serological tests can take years to decades. Read More

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Comparison of Severe Acute Respiratory Syndrome Coronavirus 2-Specific Antibodies' Binding Capacity Between Human Milk and Serum from Coronavirus Disease 2019-Recovered Women.

Breastfeed Med 2021 Apr 9. Epub 2021 Apr 9.

Department of Neonatal Immunology and Microbiology, Medolac Laboratories, A Public Benefit Corporation, Boulder City, Nevada, USA.

Human milk from coronavirus disease 2019 (COVID-19)-recovered women may be useful as oral antibody therapy to prevent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and provide long-term immunity to neonates and young children. As convalescent plasma is already used as antibody therapy, this study aimed to compare the binding capacity of antibodies specific to the receptor-binding domain (RBD) of SARS-CoV-2 between human milk and serum from COVID-19-recovered women. The areas under the curve (AUCs) for IgA, IgM, and IgG specific to the SARS-CoV-2 RBD in human milk and serum samples were measured using enzyme-linked immunosorbent assay. Read More

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Effects of angiotensin II receptor blocker usage on viral load, antibody dynamics, and transcriptional characteristics among COVID-19 patients with hypertension.

J Zhejiang Univ Sci B 2021 Apr;22(4):330-340

Department of Laboratory Medicine, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.

Epidemiological evidence suggests that patients with hypertension infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are at increased risk of acute lung injury. However, it is still not clear whether this increased risk is related to the usage of renin-angiotensin system (RAS) blockers. We collected medical records of coronavirus disease 2019 (COVID-19) patients from the First Affiliated Hospital, Zhejiang University School of Medicine (Hangzhou, China), and evaluated the potential impact of an angiotensin II receptor blocker (ARB) on the clinical outcomes of COVID-19 patients with hypertension. Read More

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LC-MS/MS measurement of 26 steroid hormones in human serum and plasma samples.

J Sep Sci 2021 Apr 9. Epub 2021 Apr 9.

Beijing AB Sciex Analytical Instrument Trading Co., Ltd., Beijing, 100011, China.

The measurement of steroid hormones provided critical information in the clinical evaluation of endocrine disorders. In this study, we developed a high throughput solid-phase extraction method for the analysis of 26 steroids in human serum and plasma samples by liquid chromatography-tandem mass spectrometry. Chromatographic condition and sample preparation were optimized to achieve good separation and maximum sensitivity for these analytes. Read More

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COVID-19 serology in nephrology healthcare workers.

Wien Klin Wochenschr 2021 Apr 9. Epub 2021 Apr 9.

Division of Nephrology and Dialysis, Department of Medicine III, Medical University of Vienna, Währinger Gürtel 18-20, 1090, Vienna, Austria.

Background: Chronic kidney disease patients show a high mortality in cases of a severe acute respiratory syndrome coronavirus-2 (SARS-CoV‑2) infection. Thus, information on the sero-status of nephrology personnel might be crucial for patient protection; however, limited information exists about the presence of SARS-CoV‑2 antibodies in asymptomatic individuals.

Methods: We examined the seroprevalence of SARS-CoV‑2 IgG and IgM antibodies among healthcare workers of a tertiary care kidney center during the the first peak phase of the corona virus disease 2019 (COVID-19) crisis in Austria using an orthogonal test strategy and a total of 12 commercial nucleocapsid protein or spike glycoprotein-based assays as well as Western blotting and a neutralization assay. Read More

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Evaluation of clinical outcomes and efficacy of palonosetron and granisetron in combination with dexamethasone in Egyptian patients receiving highly emetogenic chemotherapy.

Cancer Chemother Pharmacol 2021 Apr 9. Epub 2021 Apr 9.

Medical Oncology Department, Faculty of Medicine, Tanta University, Tanta, Egypt.

Background: Chemotherapy-induced nausea and vomiting (CINV) is considered one of the most serious adverse events affecting chemotherapy-receiving cancer patients. It dramatically affects their food intake, nutritional status and more importantly their quality of life. We can observe CINV in highly emetogenic chemotherapy (HEC) such as adriamycin-cyclophosphamide combination (AC) in breast cancer patients and cisplatin-based regimens in other cancer types. Read More

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Mortality and serum hepcidin are associated with persistent and transient acute kidney injury in septic patients.

Clin Nephrol 2021 Apr 9. Epub 2021 Apr 9.

Purpose: Acute kidney injury (AKI) is a common complication of sepsis and has high mortality. The 2017 Acute Disease Quality Initiative (AQDI) workgroup proposed new definitions for AKI - transient AKI and persistent AKI; however, very little is known about the effect of transient and persistent septic AKI on short-term mortality among critically ill patients with sepsis. The purpose of this study was to assess the impact of persistent AKI on mortality and to evaluate whether serum hepcidin can predict the occurrence of persistent AKI in critically ill patients with sepsis. Read More

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Homozygous GDF2 nonsense mutations result in a loss of circulating BMP9 and BMP10 and are associated with either PAH or an "HHT-like" syndrome in children.

Mol Genet Genomic Med 2021 Apr 9:e1685. Epub 2021 Apr 9.

Department of Medicine, University of Cambridge, Cambridge, UK.

Background: Disrupted endothelial BMP9/10 signaling may contribute to the pathophysiology of both hereditary hemorrhagic telangiectasia (HHT) and pulmonary arterial hypertension (PAH), yet loss of circulating BMP9 has not been confirmed in individuals with ultra-rare homozygous GDF2 (BMP9 gene) nonsense mutations. We studied two pediatric patients homozygous for GDF2 (BMP9 gene) nonsense mutations: one with PAH (c.[76C>T];[76C>T] or p. Read More

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Transcriptomic Profiling Identifies DCBLD2 as a Diagnostic and Prognostic Biomarker in Pancreatic Ductal Adenocarcinoma.

Front Mol Biosci 2021 23;8:659168. Epub 2021 Mar 23.

Department of General Surgery, Pancreatic Disease Center, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Accumulating evidence shows that the elevated expression of DCBLD2 (discoidin, CUB and LCCL domain-containing protein 2) is associated with unfavorable prognosis of various cancers. However, the correlation of DCBLD2 expression value with the diagnosis and prognosis of pancreatic ductal adenocarcinoma (PDAC) has not yet been elucidated.

Methods: Univariate Cox regression analysis was used to screen robust survival-related genes. Read More

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Integrative Clinical, Molecular, and Computational Analysis Identify Novel Biomarkers and Differential Profiles of Anti-TNF Response in Rheumatoid Arthritis.

Front Immunol 2021 23;12:631662. Epub 2021 Mar 23.

Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Hospital Reina Sofia, Universidad de Cordoba, Córdoba, Spain.

This prospective multicenter study developed an integrative clinical and molecular longitudinal study in Rheumatoid Arthritis (RA) patients to explore changes in serologic parameters following anti-TNF therapy (TNF inhibitors, TNFi) and built on machine-learning algorithms aimed at the prediction of TNFi response, based on clinical and molecular profiles of RA patients. A total of 104 RA patients from two independent cohorts undergoing TNFi and 29 healthy donors (HD) were enrolled for the discovery and validation of prediction biomarkers. Serum samples were obtained at baseline and 6 months after treatment, and therapeutic efficacy was evaluated. Read More

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µDrop: a system for high-throughput small-angle X-ray scattering measurements of microlitre samples.

J Appl Crystallogr 2021 Feb 1;54(Pt 1):132-141. Epub 2021 Feb 1.

Institute of Inorganic Chemistry, Graz University of Technology, Stremayrgasse 9/IV, 8010 Graz, Austria.

An automatic sample changer system for measurements of large numbers of liquid samples - the µDrop Sample Changer - is presented. It is based on a robotic arm equipped with a pipetting mechanism, which is combined with a novel drop-based sample holder. In this holder a drop of liquid is suspended between two parallel plates by surface tension. Read More

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February 2021

Serum microRNAs as Biomarkers for the Noninvasive Early Diagnosis of Biliary Tract Cancer.

Int J Gen Med 2021 1;14:1185-1195. Epub 2021 Apr 1.

Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin's Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, 300060, People's Republic of China.

Background: Biliary tract cancers (BTCs) are aggressive malignancies with difficult early diagnosis and poor prognosis. Studies have shown that microRNAs (miRNAs) are expected to be biomarkers of the disease, which indicates that we can diagnose cancers according to the miRNAs that have significant changes. The aim of this study was to explore miRNA biomarkers of BTCs. Read More

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Dynamic Changes in Serum Inhibin B Levels in 6- to 24-Month-Old Children Receiving Cryptorchidism Surgery.

Int J Gen Med 2021 31;14:1173-1177. Epub 2021 Mar 31.

Department of Pediatric General Surgery, Dongguan Eighth People's Hospital (Dongguan Children's Hospital), Dongguan, 523000, People's Republic of China.

Objective: Cryptorchidism is the most common congenital disability and is defined as the absence of one or both testicles in the scrotum during fetal development. In patients with cryptorchidism, lowered serum inhibin B levels suggest testicular dysfunction. The practical application of serum inhibin B levels in childhood remains controversial. Read More

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Serum Exosomal miRNA Might Be a Novel Liquid Biopsy to Identify Leptomeningeal Metastasis in Non-Small Cell Lung Cancer.

Onco Targets Ther 2021 1;14:2327-2335. Epub 2021 Apr 1.

Department of Respiratory and Critical Care Medicine, Affiliated Jinling Hospital, Southeast University, Sch Med, Nanjing, Jiangsu, People's Republic of China.

Purpose: The survival time of patients with leptomeningeal metastasis (LM) of lung cancer is very short, and the clinical characteristics of LM are varied, making the clinical diagnosis difficult. At present, a positive CSF fluid (CSF) cytology result remains the gold standard for diagnosing LM in lung cancer; however, the process of collecting CSF is traumatic and far less convenient than blood collection. With the development in technology, an increasing number of studies prefer to use liquid biopsy to diagnose or predict the occurrence of the disease. Read More

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Protocadherin 7-Associated Membranous Nephropathy.

J Am Soc Nephrol 2021 Apr 8. Epub 2021 Apr 8.

Unité Mixte de Recherche S 1155, Sorbonne Université, Université Pierre et Marie Curie Paris 06 and Institut National de la Santé et de la Recherche Médicale, Paris, France.

Background: Membranous nephropathy (MN) results from deposition of antigen-antibody complexes along the glomerular basement membrane (GBM). PLA2R, THSD7A, NELL1, and SEMA3B account for 80%-90% of target antigens in MN.

Methods: We performed laser microdissection and mass spectrometry (MS/MS) in kidney biopsies from 135 individuals with PLA2R-negative MN, and used immunohistochemistry/immunofluorescence and confocal microscopy to confirm the MS/MS finding, detect additional cases, and localize the novel protein. Read More

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Reference intervals for glomerular filtration function markers among pregnant women of Shandong Province, east China.

Pak J Pharm Sci 2020 Sep;33(5(Special)):2445-2448

Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Shandong, China.

The aim of the study was to establish reference intervals (RIs) for glomerular filtration function markers among pregnant women of Shandong Province, east China. From Janunary 2017 to December 2018, we retrospectively analyzed serum samples from 360 pregnant women and a control cohort of 60-non-pregnant women. The glomerular filtration function markers included Cystatin C (CysC), Creatinine (Cr) and Estimated Glomerular Filtration Rate (eGFR). Read More

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September 2020