21 results match your criteria serum mpo-dna

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Elevated Myeloperoxidase-DNA Complex Levels in Sera of Patients with IgA Vasculitis.

Pathobiology 2021 Nov 23:1-6. Epub 2021 Nov 23.

Department of Dermatology, St. Marianna University School of Medicine, Kawasaki, Japan.

Introduction: IgA vasculitis is a systemic disease that results from the entrapment of circulating IgA-containing immune complexes in small-vessel walls in the skin, kidneys, and gastrointestinal tract. An excessive formation of neutrophil extracellular traps (NETs) is involved in the pathogenesis of vasculitis, especially in antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis. This study aimed to clarify whether NETs are implicated in IgA vasculitis. Read More

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November 2021

The Quantification of Extracellular Trap Cell Death-Derived Products as Diagnostic Biomarkers for Otitis Media with Antineutrophil Cytoplasmic Antibody-Associated Vasculitis and Eosinophilic Otitis Media.

Otol Neurotol 2021 Nov 19. Epub 2021 Nov 19.

Department of Otolaryngology - Head and Neck Surgery, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan.

Objective: This study aimed to quantify the cell-free deoxyribonucleic acid (DNA), citrullinated-histone H3 (cit-H3)-DNA complex, and myeloperoxidase (MPO)-DNA complex as extracellular trap cell death (ETosis)-derived products in the middle ear fluid, and to identify diagnostic biomarkers for the discrimination of antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (OMAAV) from eosinophilic otitis media (EOM).

Study Design: Prospective study.

Setting: Tertiary referral center. Read More

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November 2021

Inhibition of PAD4-mediated NET formation by cl-amidine prevents diabetes development in nonobese diabetic mice.

Eur J Pharmacol 2021 Nov 9:174623. Epub 2021 Nov 9.

College of Life Science and Technology, China Pharmaceutical University, Nanjing, China. Electronic address:

Many evidences indicated that neutrophil extracellular traps (NETs) play pathogenic roles in type 1 diabetes (T1D). Peptidylarginine deiminases 4 (PAD4) has been proved to be indispensable for generation of NETs. In the current study, we investigated whether oral administration of cl-amidine, an effective inhibitor of PAD4, protects non-obese diabetic (NOD) mice from T1D development. Read More

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November 2021

De-escalation antibiotic therapy alleviates organ injury through modulation of NETs formation during sepsis.

Cell Death Discov 2021 Nov 10;7(1):345. Epub 2021 Nov 10.

Division of Trauma and Surgical Intensive Care Unit, Affiliated Jinling Hospital, Medical School of Nanjing University, Nanjing, 210002, Jiangsu Province, PR China.

Empiric broad-spectrum antimicrobials therapy is suggested to be started immediately for sepsis patients. Empiric antimicrobial therapy should be narrowed once pathogen identification and sensitivities are established. However, the detailed mechanisms of de-escalation strategy are still unclear. Read More

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November 2021

Neutrophil extracellular traps as a novel biomarker to predict recurrence-free and overall survival in patients with primary hepatic malignancies.

HPB (Oxford) 2021 Feb 15;23(2):309-320. Epub 2020 Aug 15.

Department of Surgery, University of Pittsburgh, Pittsburgh, PA, USA. Electronic address:

Background: The incidence of primary hepatic malignancies including Hepatocellular carcinoma (HCC) and cholangiocarcinoma (CC) is on the rise. (i) Surgery remains the mainstay of potential curative treatment, however the vast majority of patients will recur and not be amenable to curative therapy. (ii) Inflammation has been associated with poor prognosis, however there is no preoperative marker that can predict recurrence-free- or overall survival. Read More

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February 2021

Neutrophil Extracellular Trap Components Associate with Infarct Size, Ventricular Function, and Clinical Outcome in STEMI.

Mediators Inflamm 2019 21;2019:7816491. Epub 2019 Oct 21.

Center for Clinical Heart Research, Oslo University Hospital Ullevål, Norway.

Background: The relevance of neutrophil extracellular traps (NETs) in acute ST-elevation myocardial infarction (STEMI) is unclear. We explored the temporal profile of circulating NET markers and their associations to myocardial injury and function and to adverse clinical events in STEMI patients.

Methods And Results: In 259 patients, blood samples were drawn before and after PCI, on day 1, and after 4 months. Read More

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Glucose associated NETosis in patients with ST-elevation myocardial infarction: an observational study.

BMC Cardiovasc Disord 2019 10 15;19(1):221. Epub 2019 Oct 15.

Center for Clinical Heart Research, Department of Cardiology, Oslo University Hospital Ullevål, PB 4956, Nydalen, 0424, Oslo, Norway.

Background: Neutrophil extracellular traps (NETs) have recently been identified as mediators in atherothrombosis. Although NETosis in general has been suggested to be glucose dependent, the transferability to patients with acute ST-elevation myocardial infarction (STEMI) is unclear. We assessed whether the NETs markers double-stranded deoxyribonucleid acid (dsDNA) and myeloperoxidase-DNA (MPO-DNA) associated with plasma glucose and the glucometabolic status in the acute phase and 3 months after a STEMI. Read More

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October 2019

Increased plasmatic NETs by-products in patients in severe obesity.

Sci Rep 2019 10 11;9(1):14678. Epub 2019 Oct 11.

Internal Medicine, Department of Medicine, "Santa Maria della Misericordia" Hospital, University of Perugia, Perugia, Italy.

Neutrophil extracellular traps (NETs) are DNAs products involved in immune process. Obesity through a low-grade chronic inflammation determines neutrophil activation, but it is still unclear its role in NETs formation. Here we analyzed the NETs levels in healthy and morbid obese, their association with anthropometric and glyco-metabolic parameters and their changes after bariatric surgery. Read More

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October 2019

Neutrophil Extracellular Traps Drive Mitochondrial Homeostasis in Tumors to Augment Growth.

Cancer Res 2019 11 13;79(21):5626-5639. Epub 2019 Sep 13.

Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania.

Neutrophil infiltration and neutrophil extracellular traps (NET) in solid cancers are associated with poorer prognosis, but the mechanisms are incompletely understood. We hypothesized that NETs enhance mitochondrial function in tumor cells, providing extra energy for accelerated growth. Metastatic colorectal cancer tissue showed increased intratumoral NETs and supranormal preoperative serum MPO-DNA, a NET marker. Read More

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November 2019

Neutrophil extracellular traps induced by IL-8 aggravate atherosclerosis via activation NF-κB signaling in macrophages.

Cell Cycle 2019 Nov 8;18(21):2928-2938. Epub 2019 Sep 8.

Department of Cardiovascular, The First Affiliated Hospital of Harbin Medical University , Harbin , Heilongjiang , China.

Here, we sought to explore the underlying role of interleukin (IL)-8 in neutrophil extracellular traps (NETs) formation during atherosclerosis (AS). The concentration of pro-inflammatory cytokines IL-8, IL-6 and IL-1β was determined by enzyme-linked immunosorbent assay (ELISA). NETs formation was evaluated by immunofluorescence and myeloperoxidase (MPO)-DNA complex ELISA. Read More

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November 2019

Critical role of neutrophil extracellular traps (NETs) in patients with Behcet's disease.

Ann Rheum Dis 2019 09 30;78(9):1274-1282. Epub 2019 May 30.

LVTS, INSERM 1148, Paris, France

Objectives: Behçet's disease (BD) is a chronic systemic vasculitis. Thrombosis is a frequent and life-threatening complication. The pathogenesis of BD is poorly understood and evidence supporting a role for primed neutrophils in BD-associated thrombotic risk is scant. Read More

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September 2019

Neutrophil Extracellular Traps May Contribute to the Pathogenesis in Adult-onset Still Disease.

J Rheumatol 2019 12 1;46(12):1560-1569. Epub 2019 May 1.

From the departments of Rheumatology, Pathology, and Microbiology, Ajou University School of Medicine, Suwon, Korea.

Objective: Release of neutrophil extracellular traps (NET) has been described as an effector mechanism of polymorphonuclear neutrophils in several inflammatory diseases. Thus, this study was performed to evaluate the role of NET in the pathogenesis of adult-onset Still disease (AOSD).

Methods: We determined the serum levels of NET molecules and investigated their associations with clinical disease activities in patients with AOSD. Read More

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December 2019

The presence of anti-neutrophil extracellular trap antibody in patients with microscopic polyangiitis.

Rheumatology (Oxford) 2019 07;58(7):1293-1298

Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Sapporo, Japan.

Objective: Although ANCA is the major autoantibody in patients with ANCA-associated vasculitis, previous studies have suggested the presence of anti-neutrophil extracellular trap (NET) antibody in patients with microscopic polyangiitis (MPA), one type of ANCA-associated vasculitis. In this study, we aimed to determine the prevalence and pathogenic role of anti-NET antibody (ANETA) in MPA.

Methods: We examined the presence or absence of ANETA in sera obtained from 19 MPA patients by indirect immunofluorescence. Read More

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Enhanced Neutrophil Extracellular Trap Formation in Acute Pancreatitis Contributes to Disease Severity and Is Reduced by Chloroquine.

Front Immunol 2019 24;10:28. Epub 2019 Jan 24.

Division of Surgical Oncology, Department of Surgery, University of Pittsburgh, Pittsburgh, PA, United States.

Neutrophil extracellular traps (NETs) are generated when activated neutrophils, driven by PAD4, release their DNA, histones, HMGB1, and other intracellular granule components. NETs play a role in acute pancreatitis, worsening pancreatic inflammation, and promoting pancreatic duct obstruction. The autophagy inhibitor chloroquine (CQ) inhibits NET formation; therefore, we investigated the impact of CQ mediated NET inhibition in murine models of pancreatitis and human correlative studies. Read More

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December 2019

The Diagnostic and Clinical Utility of the Myeloperoxidase-DNA Complex as a Biomarker in Otitis Media With Antineutrophil Cytoplasmic Antibody-associated Vasculitis.

Otol Neurotol 2019 02;40(2):e99-e106

Department of Otolaryngology - Head and Neck Surgery.

Objective: This prospective study aimed to evaluate the diagnostic and clinical utility of the myeloperoxidase (MPO)-DNA complex as a NETosis-derived product in the middle ear fluid of patients with otitis media with antineutrophil cytoplasmic antibody-associated vasculitis (OMAAV).

Study Design: Prospective study.

Setting: Tertiary referral center. Read More

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February 2019

Quantification of NETs-associated markers by flow cytometry and serum assays in patients with thrombosis and sepsis.

Int J Lab Hematol 2018 Aug 9;40(4):392-399. Epub 2018 Mar 9.

Department of Haematology, St George Hospital, Kogarah, NSW, Australia.

Introduction: Neutrophil extracellular traps (NETs) are networks of extracellular fibres produced from neutrophil DNA with a pathogenic role in infection, thrombosis and other conditions. Reliable assays for measuring NETs are desirable as novel treatments targeting NETs are being explored for the treatment of these conditions. We compare a whole blood flow cytometry method with serum assays to measure NETs-associated markers in patients with sepsis and thrombosis. Read More

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Increased levels of neutrophil extracellular trap remnants in the serum of patients with rheumatoid arthritis.

Int J Rheum Dis 2018 Feb 6;21(2):415-421. Epub 2017 Nov 6.

Department of Clinical Laboratory, The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan, China.

Aims: Neutrophil extracellular traps (NETs) comprise a unique form of non-apoptotic cell death exhibited by neutrophils, which occurs in a stepwise process termed NETosis. It has been postulated that NETosis plays an important role in the pathogenesis of autoimmune disorders. The aim of this study was to evaluate serum levels of NET remnants in patients with rheumatoid arthritis (RA), as well as potential associations between NET remnants and indicators of RA. Read More

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February 2018

IL-33 exacerbates liver sterile inflammation by amplifying neutrophil extracellular trap formation.

J Hepatol 2017 Sep 21. Epub 2017 Sep 21.

Department of Surgery, University of Pittsburgh, Pittsburgh, PA, United States; Department of Surgery, Union Hospital, Huazhong University of Science and Technology, Wuhan, PR China. Electronic address:

Background & Aims: Neutrophils and liver sinusoidal endothelial cells (LSECs) both contribute to sterile inflammatory injury during ischemia/reperfusion (I/R), a well-known liver surgical stress. Interleukin-33 (IL-33) has been shown to drive neutrophil infiltration during inflammatory responses through its receptor ST2. We recently reported that infiltrating neutrophils form neutrophil extracellular traps (NETs), which exacerbate sterile inflammatory injury in liver I/R. Read More

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September 2017

Superoxide induces Neutrophil Extracellular Trap Formation in a TLR-4 and NOX-dependent mechanism.

Mol Med 2016 Oct 18;22:621-631. Epub 2016 Jul 18.

Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA, USA.

Neutrophils constitute the early innate immune response to perceived infectious and sterile threats. Neutrophil Extracellular Traps (NETs) are a novel mechanism to counter pathogenic invasion and sequelae of ischemia including cell death and oxidative stress. Superoxide is a radical intermediate of oxygen metabolism produced by parenchymal and non-parenchymal hepatic cells, and is a hallmark of oxidative stress after liver ischemia-reperfusion (I/R). Read More

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October 2016

Circulating Neutrophil Extracellular Trap Levels in Well-Controlled Type 2 Diabetes and Pathway Involved in Their Formation Induced by High-Dose Glucose.

Pathobiology 2016 18;83(5):243-51. Epub 2016 May 18.

Division of Rheumatology, Endocrinology and Nephrology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.

Objectives: Although intensive therapy for type 2 diabetes (T2D) prevents microvascular complications, 10% of well-controlled T2D patients develop microangiopathy. Therefore, the identification of risk markers for microvascular complications in well-controlled T2D patients is important. Recent studies have demonstrated that high-dose glucose induces neutrophil extracellular trap (NET) formation, which can be a risk for microvascular disorders. Read More

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January 2017

Circulating Level of Neutrophil Extracellular Traps Is Not a Useful Biomarker for Assessing Disease Activity in Antineutrophil Cytoplasmic Antibody-Associated Vasculitis.

PLoS One 2016 3;11(2):e0148197. Epub 2016 Feb 3.

Renal Division, Department of Medicine, Peking University, First Hospital, Beijing 100034, China.

Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) is a group of life-threatening disorders, and frequently affects the kidneys. This study investigated whether the circulating neutrophil extracellular traps (NETs) levels were associated with disease activity of AAV. We collected serum samples from 34 patients with AAV in active stage and 62 patients with AAV in remission. Read More

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