262 results match your criteria serial sacrifice


Genome engineering of Nannochloropsis with hundred-kilobase fragment deletions by Cas9 cleavages.

Plant J 2021 Mar 14. Epub 2021 Mar 14.

Single-Cell Center, CAS Key Laboratory of Biofuels, Shandong Key Laboratory of Energy Genetics and Shandong Institute of Energy Research, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China.

Industrial microalgae are promising photosynthetic cell factories, yet tools for large-scale targeted genome engineering are limited. Here for the model industrial oleaginous microalga Nannochloropsis oceanica, we established a method to precisely and serially delete large genome fragments of ~100 kb from its 30.01 Mb nuclear genome. Read More

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Super-resolution head and neck MRA using deep machine learning.

Magn Reson Med 2021 Jul 22;86(1):335-345. Epub 2021 Feb 22.

Department of Radiology, NorthShore University HealthSystem, Evanston, Illinois, USA.

Purpose: To probe the feasibility of deep learning-based super-resolution (SR) reconstruction applied to nonenhanced MR angiography (MRA) of the head and neck.

Methods: High-resolution 3D thin-slab stack-of-stars quiescent interval slice-selective (QISS) MRA of the head and neck was obtained in eight subjects (seven healthy volunteers, one patient) at 3T. The spatial resolution of high-resolution ground-truth MRA data in the slice-encoding direction was reduced by factors of 2 to 6. Read More

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Coronary microcirculation damage in anthracycline cardiotoxicity.

Cardiovasc Res 2021 Feb 19. Epub 2021 Feb 19.

Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain.

Aims: The aim of this study was to study changes in coronary microcirculation status during and after several cycles of anthracycline treatment.

Methods And Results: Large-White male pigs (n = 40) were included in different experimental protocols (ExPr.) according to anthracycline cumulative exposure (0. Read More

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February 2021

Cardiac repair in a murine model of myocardial infarction with human induced pluripotent stem cell-derived cardiomyocytes.

Stem Cell Res Ther 2020 07 17;11(1):297. Epub 2020 Jul 17.

Bioisland Laboratory, Biomedical Equipment Department, Building 3, No.188 KaiYuan Road, Huangpu District, Guangzhou, Guangdong, China.

Background: Cellular replacement strategies using human induced pluripotent stem cells (iPSCs) and their cardiac derivatives are emerging as novel treatments for post-myocardial infarction (MI) heart failure (HF); however, the mechanism of recovery of heart function is not very clear. The purpose of this study was to investigate the efficiency of using highly purified human induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs) for myocardial repair in a mouse model of MI and to clarify the mechanism of recovery of heart function.

Methods: Animals modelling MI were randomly assigned to receive direct intramyocardial injection of culture medium (MI group) or 4 × 10 iPS-CMs (cell group) at the infarct border zone. Read More

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Remote ischaemic preconditioning ameliorates anthracycline-induced cardiotoxicity and preserves mitochondrial integrity.

Cardiovasc Res 2021 Mar;117(4):1132-1143

Translational Laboratory for Cardiovascular Imaging and Therapy, Centro Nacional de Investigaciones Cardiovasculares (CNIC), c/Melchor Fernandez Almagro, 3. 28029 Madrid, Spain.

Aims: Anthracycline-induced cardiotoxicity (AIC) is a serious adverse effect among cancer patients. A central mechanism of AIC is irreversible mitochondrial damage. Despite major efforts, there are currently no effective therapies able to prevent AIC. Read More

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A 100 bp GAGA motif-containing sequence in AGAMOUS second intron is able to suppress the activity of CaMV35S enhancer in vegetative tissues.

PLoS One 2020 5;15(3):e0230203. Epub 2020 Mar 5.

Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops; Biotechnology Research Center, Southwest University, Beibei, Chongqing, P. R. China.

Flower-specific promoters enable genetic manipulation of floral organs to improve crop yield and quality without affecting vegetative growth. However, the identification of strong tissue-specific promoters is a challenge. In addition, information on cis elements that is able to repress gene expression in vegetative tissues remains limited. Read More

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Endoscope-assisted Partial Cochlectomy for Intracochlear Schwannoma With Simultaneous Cochlear Implantation: A Case Report.

Otol Neurotol 2020 03;41(3):334-338

Sydney Endoscopic Ear Surgery (SEES) Research Group.

Introduction: Intralabyrinthine schwannomas are a small subset of vestibular schwannomas which originate within the labyrinthine structures. Management typically consists of watch-and-wait strategies given that surgical intervention will sacrifice hearing. Endoscopic resection of primary intracochlear schwannoma with simultaneous cochlear implantation for a patient with progressive hearing loss and debilitating tinnitus is described. Read More

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Pathological Study on Epithelial-Mesenchymal Transition in Silicotic Lung Lesions in Rat.

Vet Sci 2019 Aug 30;6(3). Epub 2019 Aug 30.

Department of Environmental Engineering, Kyoto University Graduate School of Engineering, Kyoto-shi, Kyoto 615-8530, Japan.

Silicosis, caused by the inhalation of crystalline silicon dioxide or silica, is one of the most severe occupational diseases. Persistent inflammation and progressive massive pulmonary fibrosis are the most common histological changes caused by silicosis. Association of epithelial-mesenchymal transition (EMT) of hyperplastic type II epithelial cells with the fibrotic events of pulmonary fibrosis has been suggested in in vitro silica-exposed cultured cell models, patients with idiopathic pulmonary fibrosis, and bleomycin-induced experimental models. Read More

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Serial Magnetic Resonance Imaging to Identify Early Stages of Anthracycline-Induced Cardiotoxicity.

J Am Coll Cardiol 2019 02;73(7):779-791

Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain; Centro de Investigación Biomédica en Red en Enfermedades Cardiovasculares (CIBERCV), Madrid, Spain; Cardiology Department, IIS-Fundación Jiménez Díaz Hospital, Madrid, Spain. Electronic address:

Background: Anthracycline-induced cardiotoxicity is a major clinical problem, and early cardiotoxicity markers are needed.

Objectives: The purpose of this study was to identify early doxorubicin-induced cardiotoxicity by serial multiparametric cardiac magnetic resonance (CMR) and its pathological correlates in a large animal model.

Methods: Twenty pigs were included. Read More

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February 2019

In vivo evaluation of resorbable supercritical CO -treated collagen membranes for class III furcation-guided tissue regeneration.

J Biomed Mater Res B Appl Biomater 2019 07 10;107(5):1320-1328. Epub 2018 Sep 10.

Department of Biomaterials and Biomimetics, New York University College of Dentistry, New York, New York.

The study evaluated the effects of a Supercritical CO (scCO ) on a commercially available decellularized/delipidized naturally derived porcine pericardium collagen membrane, Vitala®. The Vitala® and scCO treated experimental membranes were evaluated for guided tissue regeneration (GTR) of periodontal tissue in class III furcation defects utilizing a dog model. Physical material characterization was performed by scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). Read More

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Tracking embryonic hematopoietic stem cells to the bone marrow: nanoparticle options to evaluate transplantation efficiency.

Stem Cell Res Ther 2018 07 27;9(1):204. Epub 2018 Jul 27.

Department of Biomedical Engineering, University of Iowa, 5601 Seamans Center for the Engineering Arts and Sciences, Iowa City, IA, 52242, USA.

Background: As the prevalence of therapeutic approaches involving transplanted cells increases, so does the need to noninvasively track the cells to determine their homing patterns. Of particular interest is the fate of transplanted embryonic stem cell-derived hematopoietic progenitor cells (HPCs) used to restore the bone marrow pool following sublethal myeloablative irradiation. The early homing patterns of cell engraftment are not well understood at this time. Read More

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Targeted inhibition of Axl receptor tyrosine kinase ameliorates anti-GBM-induced lupus-like nephritis.

J Autoimmun 2018 09 9;93:37-44. Epub 2018 Jun 9.

Division of Immunology, Allergy and Rheumatology, Department of Internal Medicine, University of Cincinnati, Cincinnati, OH 45267 USA. Electronic address:

Glomerulonephritis (GN) is a typical lesion in autoantibody and immune complex disorders, including SLE. Because the Gas6/Axl pathway has been implicated in the pathogenesis of many types of GN, targeting this pathway might ameliorate GN. Consequently, we have studied the efficacy and mechanism of R428, a potent selective Axl inhibitor, in the prevention of experimental anti-GBM nephritis. Read More

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September 2018

siRNA Delivery and Rebound of Renal in Mice.

J Drug Deliv 2017 20;2017:4070793. Epub 2017 Dec 20.

Department of Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, USA.

siRNA stabilized for applications is filtered and reabsorbed in the renal proximal tubule (PT), reducing mRNA expression transiently. Prior siRNA efforts have successfully prevented upregulation of mRNA in response to injury. We proposed reducing constitutive gene and protein expression of (megalin) in order to understand its molecular regulation in mice. Read More

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December 2017

Hypoxia-inducible factor 1 in clinical and experimental aortic aneurysm disease.

J Vasc Surg 2018 11 11;68(5):1538-1550.e2. Epub 2017 Dec 11.

Department of Surgery, Stanford University School of Medicine, Stanford, Calif. Electronic address:

Objective: Mural angiogenesis and macrophage accumulation are two pathologic hallmarks of abdominal aortic aneurysm (AAA) disease. The heterodimeric transcription factor hypoxia-inducible factor 1 (HIF-1) is an essential regulator of angiogenesis and macrophage function. In this study, we investigated HIF-1 expression and activity in clinical and experimental AAA disease. Read More

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November 2018

Spinal ischaemia after thoracic endovascular aortic repair with left subclavian artery sacrifice: is there a critical stent graft length?

Eur J Cardiothorac Surg 2018 02;53(2):385-391

Department of Cardiovascular Surgery, Heart Center Freiburg University, Freiburg, Germany.

Objectives: Thoracic endovascular aortic repair (TEVAR) is used for treatment of thoracic aortic pathologies, but the covered stent graft can induce spinal ischaemia depending on the length used. The left subclavian artery contributes to spinal cord collateralization and is frequently occluded by the stent graft. Our objective was to investigate the impact of covered stent graft length on the risk of spinal ischaemia in the setting of left subclavian artery sacrifice. Read More

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February 2018

Intramyocardial bone marrow mononuclear cells versus bone marrow-derived and adipose mesenchymal cells in a rat model of dilated cardiomyopathy.

Cytotherapy 2017 08 30;19(8):947-961. Epub 2017 Jun 30.

Maimonides Institute of Biomedical Research in Cordoba (IMIBIC), Spain; Cellular Therapy Unit, Reina Sofia University Hospital, Cordoba, Spain; University of Cordoba, Spain; Hematology Department, Reina Sofia University Hospital, Cordoba, Spain.

Background: Effects of cell therapy on dilated cardiomyopathy (DCM) have been investigated in pre-clinical models using distinct cellular types in each study. A single study that compares the effectiveness of different cells is lacking.

Methods: We have compared the effects of intramyocardial injection (IMI) of bone marrow (BM)-derived mononuclear cells (MNCs), BM and adipose tissue (AT) mesenchymal stromal cells (BM-MSCs and AT-MSCs) on heart function, histological changes and myocardial ultrastructure in a rat model of DCM. Read More

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Acute and chronic changes in rat soleus muscle after high-fat high-sucrose diet.

Physiol Rep 2017 May;5(10):e13270

Human Performance Laboratory, University of Calgary, Calgary, Alberta, Canada.

The effects of obesity on different musculoskeletal tissues are not well understood. The glycolytic quadriceps muscles are compromised with obesity, but due to its high oxidative capacity, the soleus muscle may be protected against obesity-induced muscle damage. To determine the time-course relationship between a high-fat/high-sucrose (HFS) metabolic challenge and soleus muscle integrity, defined as intramuscular fat invasion, fibrosis and molecular alterations over six time points. Read More

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Characterization of a novel caudal vertebral interbody fusion in a rat tail model: An implication for future material and mechanical testing.

Biomed J 2017 Feb 15;40(1):62-68. Epub 2017 Mar 15.

Department of Orthopaedic Surgery, Spine Section, Musculoskeletal Research Center, Chang Gung Memorial Hospital at Linkou, Chang Gung University College of Medicine, Taoyuan, Taiwan.

Background: Of the proposed animal interbody fusion models, rat caudal discs have gained popularity in disc research due to their strong resemblance to human discs with respect to geometry, composition and mechanical properties. The purpose of this study is to demonstrate an efficient, repeatable and easily accessible animal model of interbody fusion for future research into mechanical testing and graft materials.

Methods: Twelve 12-week-old female Sprague-Dawley (SD) rats underwent caudal interbody fusion of the third and fourth coccygeal vertebrae of the tail. Read More

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February 2017

Contralateral artery enlargement predicts carotid plaque progression based on machine learning algorithm models in apoE mice.

Biomed Eng Online 2016 Dec 28;15(Suppl 2):146. Epub 2016 Dec 28.

Department of Cardiology, Zhongda Hospital, Medical School of Southeast University, 87 Dingjiaqiao, Nanjing, 210009, Jiangsu, China.

Background: This study specifically focused on anatomical MRI characterization of the low shear stress-induced atherosclerotic plaque in mice. We used machine learning algorithms to analyze multiple correlation factors of plaque to generate predictive models and to find the predictive factor for vulnerable plaque.

Methods: Branches of the left carotid artery in apoE and C57BL/6J mice were ligated to produce the partial left carotid artery model. Read More

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December 2016

Local Coverage for Acute Severely Herniated Brain Using Scalp and Forehead Flaps in a Pediatric Patient With Head Trauma.

J Craniofac Surg 2017 Jan;28(1):231-235

*Division of Plastic Surgery †Department of Neurosurgery, MetroHealth Medical Center, Case Western Reserve University, Cleveland, OH.

A 4-year-old-boy required emergent craniectomy and drainage of a large right-sided acute subdural hematoma after head trauma, during which massive sinus hemorrhage with brain swelling occurred. Acute intraoperative management entailed dural cover using synthetic dural membrane substitute and scalp coverage over the herniated brain using acellular dermal matrix. After intensive monitoring and control of raised intracranial pressure over the next few days, the exposed brain was then covered by scalp and forehead flaps with cadaveric skin grafting to the donor site. Read More

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January 2017

Efficacy of protocols for induction of chronic hyperthyroidism in male and female mice.

Endocrine 2016 Oct 29;54(1):47-54. Epub 2016 Jul 29.

Department of Endocrinology and Metabolism, University Hospital Essen, University Duisburg-Essen, 45122, Essen, Germany.

Protocols for induction of hyperthyroidism in mice are highly variable and mostly involve short-term thyroid hormone (TH) treatment. In addition, little is known about a possible influence of sex on experimental TH manipulation. Here we analyzed the efficacy of intraperitoneal vs. Read More

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October 2016

Validation of a Radiography-Based Quantification Designed to Longitudinally Monitor Soft Tissue Calcification in Skeletal Muscle.

PLoS One 2016 20;11(7):e0159624. Epub 2016 Jul 20.

Department of Orthopaedics and Rehabilitation, Vanderbilt University Medical Center, Medical Center East, South Tower, Suite 4200, 1215 21th Avenue South, Nashville TN, 37232, United States of America.

Introduction: Soft tissue calcification, including both dystrophic calcification and heterotopic ossification, may occur following injury. These lesions have variable fates as they are either resorbed or persist. Persistent soft tissue calcification may result in chronic inflammation and/or loss of function of that soft tissue. Read More

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Characterization of a nose-only inhaled phosgene acute lung injury mouse model.

Inhal Toxicol 2015 ;27(14):832-40

a Biomedical Research Center, Battelle - LSR , Columbus , OH , USA and.

Context: Phosgene's primary mode of action is as a pulmonary irritant characterized by its early latent phase where life-threatening, non-cardiogenic pulmonary edema is typically observed 6-24 h post-exposure.

Objective: To develop an inhaled phosgene acute lung injury (ALI) model in C57BL/6 mice that can be used to screen potential medical countermeasures.

Methods: A Cannon style nose-only inhalation exposure tower was used to expose mice to phosgene (8 ppm) or air (sham). Read More

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September 2016

γ-H2AX Kinetic Profile in Mouse Lymphocytes Exposed to the Internal Emitters Cesium-137 and Strontium-90.

PLoS One 2015 30;10(11):e0143815. Epub 2015 Nov 30.

Center for Radiological Research, Columbia University Medical Center, New York, New York, United States of America.

In the event of a dirty bomb scenario or an industrial nuclear accident, a significant dose of volatile radionuclides such as 137Cs and 90Sr may be dispersed into the atmosphere as a component of fallout and inhaled or ingested by hundreds and thousands of people. To study the effects of prolonged exposure to ingested radionuclides, we have performed long-term (30 day) internal-emitter mouse irradiations using soluble-injected 137CsCl and 90SrCl2 radioisotopes. The effect of ionizing radiation on the induction and repair of DNA double strand breaks (DSBs) in peripheral mouse lymphocytes in vivo was determined using the γ-H2AX biodosimetry marker. Read More

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Safety and Biodistribution Evaluation in Cynomolgus Macaques of rAAV2tYF-CB-hRS1, a Recombinant Adeno-Associated Virus Vector Expressing Retinoschisin.

Hum Gene Ther Clin Dev 2015 Sep;26(3):165-76

1 Applied Genetic Technologies Corporation , Alachua, Florida.

Applied Genetic Technologies Corporation is developing rAAV2tYF-CB-hRS1, a recombinant adeno-associated virus (rAAV) vector for treatment of X-linked retinoschisis (XLRS), an inherited retinal disease characterized by splitting (schisis) of retinal layers causing poor vision. We report here results of a study evaluating the safety and biodistribution of rAAV2tYF-CB-hRS1 in normal cynomolgus macaques. Three groups of male animals (n = 6 per group) received an intravitreal injection in one eye of either vehicle, or rAAV2tYF-CB-hRS1 at one of two dose levels (4 × 10(10) or 4 × 10(11) vg/eye). Read More

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September 2015

Comparison of Monkeypox Virus Clade Kinetics and Pathology within the Prairie Dog Animal Model Using a Serial Sacrifice Study Design.

Biomed Res Int 2015 24;2015:965710. Epub 2015 Aug 24.

Centers for Disease Control and Prevention, Poxvirus and Rabies Branch, Atlanta, GA 30333, USA.

Monkeypox virus (MPXV) infection of the prairie dog is valuable to studying systemic orthopoxvirus disease. To further characterize differences in MPXV clade pathogenesis, groups of prairie dogs were intranasally infected (8 × 10(3) p.f. Read More

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(18)F-fluoromisonidazole PET reveals spatial and temporal heterogeneity of hypoxia in mouse models of human non-small-cell lung cancer.

Future Oncol 2015 11;11(20):2841-9. Epub 2015 Sep 11.

Department of Radiology, University of Louisville School of Medicine, Louisville, KY 40202, USA.

Aim: To noninvasively observe dynamic changes in tumor hypoxia in mouse models of human non-small-cell lung cancer (NSCLC) using (18)F-fluoromisonidazole PET.

Materials & Methods: Nude mice with NSCLC H460 and A549 subcutaneous xenografts were coinjected intravenously with (18)F-fluoromisonidazole and the hypoxia marker pimonidazole, and observed by serial PET scans. After sacrifice, the tumor distribution of (18)F-fluoromisonidazole and pimonidazole was compared by digital autoradiography and microscopy, respectively. Read More

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Treatment of Acute Vocal Fold Injury With Platelet-Rich Plasma.

J Voice 2016 Nov 17;30(6):731-735. Epub 2015 Aug 17.

Department of Otolaryngology, Selcuklu Faculty of Medicine, Selcuk University, Konya, Turkey.

Objectives: Platelet-rich plasma (PRP) is a reliable and has low side-effect profile and has beneficial effects on wound healing. Its investigatory effects on wound-healing process were shown on various tissues. The aim of the present study was to evaluate effectiveness of PRP application on scar tissue of acute vocal fold injury. Read More

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November 2016

Effect of High-Calcium Diet on Coronary Artery Disease in Ossabaw Miniature Swine With Metabolic Syndrome.

J Am Heart Assoc 2015 Aug 13;4(8):e001620. Epub 2015 Aug 13.

Department of Nutrition Science, Purdue University, West Lafayette, IN (A.K.P.E., M.E.W., B.R.M., C.M.W.).

Background: Calcium is a shortfall essential nutrient that has been a mainstay of osteoporosis management. Recent and limited findings have prompted concern about the contribution of calcium supplementation to cardiovascular risk. A proposed mechanism is through the acceleration of coronary artery calcification. Read More

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Monitoring brain development of chick embryos in vivo using 3.0 T MRI: subdivision volume change and preliminary structural quantification using DTI.

BMC Dev Biol 2015 Jul 25;15:29. Epub 2015 Jul 25.

Department of Radiology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Background: Magnetic resonance imaging (MRI) has many advantages in the research of in vivo embryonic brain development, specifically its noninvasive aspects and ability to avoid skeletal interference. However, few studies have focused on brain development in chick, which is a traditional animal model in developmental biology. We aimed to serially monitor chick embryo brain development in vivo using 3. Read More

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