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Structural features and phylogenetic implications of Cicadellidae subfamily and two new mitogenomes leafhoppers.

PLoS One 2021 14;16(5):e0251207. Epub 2021 May 14.

School of Karst Science, Guizhou Normal University/State Key Laboratory Cultivation Base for Guizhou Karst Mountain Ecology Environment of China, Guiyang, China.

Complete mitochondrial genome sequences facilitate species identification and analyses of phylogenetic relationships. However, the available data are limited to the diverse and widespread insect family Cicadellidae. This study analyzes and summarizes the complete mitochondrial genome structure characteristics of 11 leafhopper subfamilies and two newly sequenced Typhlocybinae species, Empoascanara wengangensis and E. Read More

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PCR detection and genetic characterization of piroplasms from dogs in Myanmar, and a possible role of dogs as reservoirs for Theileria parasites infecting cattle, water buffaloes, and goats.

Ticks Tick Borne Dis 2021 Apr 21;12(4):101729. Epub 2021 Apr 21.

Laboratory of Parasitology, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan. Electronic address:

Canine vector-borne pathogens can act as zoonotic agents in humans; however, it poorly understood whether dogs play a role as reservoirs of vector-borne parasites in livestock animals. Here, we report the unexpected detection of 18S rRNA gene (rDNA) sequences of five ruminant Theileria species from the peripheral blood of dogs in Myanmar, in addition to those of two canine Babesia species. Using novel BTH primers capable of amplifying the 18S rDNA of Babesia, Theileria, and Hepatozoon spp. Read More

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(Urticaceae), a new species from limestone karst in Guangxi, China.

PeerJ 2021 19;9:e11148. Epub 2021 Apr 19.

Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, China.

L.F. Fu, A. Read More

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Detection of simultaneous production of kurstakin, fengycin and surfactin lipopeptides in Bacillus mojavensis using a novel gel-based method and MALDI-TOF spectrometry.

World J Microbiol Biotechnol 2021 May 10;37(6):97. Epub 2021 May 10.

Department of Cellular and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran.

Bacterial lipopeptides have become a research focus of many studies owing to their industrial and pharmaceutical importance. Although such studies focused on researching purification procedures and qualitative analysis, much remains to be explored and developed to improve the current methods. To enable thorough studies of lipopeptides, this paper describes a new method for purification and characterization of in-gel anionic lipopeptides. Read More

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The complete mitochondrial genome sequences of varieties NY18 and 088018.

Mitochondrial DNA B Resour 2021 Apr 15;6(4):1429-1430. Epub 2021 Apr 15.

Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture and Rural Affairs, Nanjing, China.

variety NY18 and 088018 are female and male parents of the national registered variety Ningza 1818, respectively. Here, we determined the complete mitochondrial genomes of these two varieties. The genome sizes of NY18 and 088018 were 221,864 bp and 222,015 bp, respectively. Read More

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Complete chloroplast genome of and its phylogenetics relationships in Anacardiaceae family.

Physiol Mol Biol Plants 2021 Apr 11;27(4):801-814. Epub 2021 Apr 11.

Biotechnology Institute (IBTEC), UNESP-Univ Estadual Paulista, Botucatu, SP CEP 18607-440 Brazil.

Continuous exploratory use of tree species is threatening the existence of several plants in South America. One of these threatened species is , highly exploited due to the high quality and durability of its wood. The chloroplast (cp) has been used for several evolutionary studies as well traceability of timber origin, based on its gene sequences and simple sequence repeats (SSR) variability. Read More

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Genetic polymorphism of 23 autosomal STR loci in Han population from Yuncheng, Shanxi Province.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2021 Apr;46(4):351-360

Key Laboratory of Cell Engineering in Guizhou Province; Affiliated Hospital of Zunyi Medical University, Zunyi Guizhou 563099.

Objectives: Due to the genetic feature of high diversity than other DNA markers, short tandem repeat (STR) plays key roles in forensic, anthropology, and population genetics. Newly introduced multiple STR kit is more valuable because of the greatly improved discriminatory power with the increase in the number of STR loci. The genetic polymorphic data are essential for the application and research in specific population. Read More

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Estimating the Period Prevalence of Publicly Funded Abortion to Space Live Births, 1999 to 2014.

J Prim Care Community Health 2021 Jan-Dec;12:21501327211012182

American Association of Pro-Life Obstetricians and Gynecologists, Eau Claire, MI, USA.

Introduction/objectives: Although a majority of women who have an abortion report having 1 or more children, there is no published research on the number of abortions which occur between live births, after a first child but before the last. The objectives of this research, therefore, were to estimate the period prevalence of an induced abortion separating live births in a population of Medicaid eligible enrollees and to identify the characteristics of enrollees significantly associated with the use of abortion to enable child spacing.

Methods: A retrospective, cross-sectional, longitudinal analysis of the pregnancy outcome sequences of eligible enrollees over age 13 from the 17 states where Medicaid included coverage of all abortions, with at least one identifiable pregnancy outcome between 1999 and 2014. Read More

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Diverse RNA Viruses Discovered in Three Parasitoid Wasps of the Rice Weevil .

mSphere 2021 May 5;6(3). Epub 2021 May 5.

State Key Laboratory of Rice Biology, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, China

In this study, many virus-like fragments were obtained from transcriptomes of three wasp species, including (8), (3), and (18), which can parasitize and control rice weevil , a serious insect pest of farm-stored grains. By further bioinformatic analysis and sequencing, we identified six novel RNA viruses with complete genomes and named them WWPSRV-1, WWPSRV-2, AcPSRV-1, AcNSRV-1, AcNSRV-2, and LdNSRV-1. PCR-based detection revealed that WWPSRV-1 and WWPSRV-2 had the possibility of interspecies virus transmission, especially WWPSRV-2, which was also present in the rice weevil adults. Read More

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Complete genome sequence analysis of a novel coguvirus isolated from Paris polyphylla var. yunnanensis.

Arch Virol 2021 May 4. Epub 2021 May 4.

Key Laboratory for Agricultural Biodiversity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, No. 95, Jinhei Road, Panlong District, Kunming, 650201, Yunnan, China.

A novel negative-stranded (ns) RNA virus tentatively named "Yunnan paris negative-stranded virus" (YPNSV), was isolated from Paris polyphylla var. yunnanensis plants exhibiting leaf chlorosis and mosaic symptoms in Yunnan. Its complete genome sequence was determined using Illumina and Sanger sequencing. Read More

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Auditory streaming emerges from fast excitation and slow delayed inhibition.

J Math Neurosci 2021 May 3;11(1). Epub 2021 May 3.

Department of Mathematics, College of Engineering, Mathematics & Physical Sciences, University of Exeter, Exeter, UK.

In the auditory streaming paradigm, alternating sequences of pure tones can be perceived as a single galloping rhythm (integration) or as two sequences with separated low and high tones (segregation). Although studied for decades, the neural mechanisms underlining this perceptual grouping of sound remains a mystery. With the aim of identifying a plausible minimal neural circuit that captures this phenomenon, we propose a firing rate model with two periodically forced neural populations coupled by fast direct excitation and slow delayed inhibition. Read More

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Decoding first complete chloroplast genome of toothbrush tree (Salvadora persica L.): insight into genome evolution, sequence divergence and phylogenetic relationship within Brassicales.

BMC Genomics 2021 Apr 30;22(1):312. Epub 2021 Apr 30.

Natural and Medical Sciences Research Center, University of Nizwa, 616, Nizwa, Oman.

Background: Salvadora persica L. (Toothbrush tree - Miswak; family-Salvadoraceae) grows in the arid-land ecosystem and possesses economic and medicinal importance. The species, genus and the family have no genomic datasets available specifically on chloroplast (cp) genomics and taxonomic evolution. Read More

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The omega-loop of cobra cytotoxins tolerates multiple amino acid substitutions.

Biochem Biophys Res Commun 2021 Jun 26;558:141-146. Epub 2021 Apr 26.

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya str., Moscow, 117997, Russia.

Cobra cytotoxins (CTs), the three-fingered proteins, feature high amino acid sequence homology in the beta-strands and variations in the loop regions. We selected a pair of cytotoxins from Naja kaouthia crude venom to clarify the sequence-structure relationships. Using chromatography and mass spectroscopy, we separated and identified the mixture of cytotoxins 2 and 3, differentiated by the only Val 41/Ala 41 substitution. Read More

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Whole genome-based characterisation of antimicrobial resistance and genetic diversity in Campylobacter jejuni and Campylobacter coli from ruminants.

Sci Rep 2021 Apr 26;11(1):8998. Epub 2021 Apr 26.

Animal Health Department, NEIKER - Basque Institute for Agricultural Research and Development, Basque Research and Technology Alliance (BRTA), Bizkaia Science and Technology Park 812L, 48160, Derio, Bizkaia, Spain.

Campylobacter, a leading cause of gastroenteritis in humans, asymptomatically colonises the intestinal tract of a wide range of animals.Although antimicrobial treatment is restricted to severe cases, the increase of antimicrobial resistance (AMR) is a concern. Considering the significant contribution of ruminants as reservoirs of resistant Campylobacter, Illumina whole-genome sequencing was used to characterise the mechanisms of AMR in Campylobacter jejuni and Campylobacter coli recovered from beef cattle, dairy cattle, and sheep in northern Spain. Read More

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Description of Paraxiopsis/ kensleyi/ n. sp., a new axiid lobster from the Gulf of Mexico (Crustacea: Decapoda: Axiidea).

Authors:
Darryl L Felder

Zootaxa 2021 Apr 27;4965(1):zootaxa.4965.1.6. Epub 2021 Apr 27.

Department of Biology, University of Louisiana at Lafayette, Lafayette 70504-3602 USA.

A new species of axiid was discovered during sampling offshore deep banks on the middle continental shelf off Louisiana. Paraxiopsis kensleyi n. sp. Read More

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Sulfitobacter alexandrii sp. nov., a new microalgae growth-promoting bacterium with exopolysaccharides bioflocculanting potential isolated from marine phycosphere.

Antonie Van Leeuwenhoek 2021 Apr 24. Epub 2021 Apr 24.

Department of Marine Chemistry, Zhejiang Ocean University, Zhoushan, China.

Marine phycosphere harbors unique cross-kingdom associations with enormous ecological significance in aquatic ecosystems as well as relevance for algal biotechnology industry. During our investigating the microbial composition and bioactivity of marine phycosphere microbiota (PM), a novel lightly yellowish and versatile bacterium designated strain AM1-D1 was isolated from cultivable PM of marine dinoflagellate Alexandrium minutum amtk4 that produces high levels of paralytic shellfish poisoning toxins (PSTs). Strain AM1-D1 demonstrates notable bioflocculanting bioactivity with bacterial exopolysaccharides (EPS), and microalgae growth-promoting (MGP) potential toward its algal host. Read More

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A small interfering RNA (siRNA) database for SARS-CoV-2.

Sci Rep 2021 04 23;11(1):8849. Epub 2021 Apr 23.

Bioinformatics Graduate Program, Metrópole Digital Institute, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, 59078-400, Brazil.

Coronavirus disease 2019 (COVID-19) rapidly transformed into a global pandemic, for which a demand for developing antivirals capable of targeting the SARS-CoV-2 RNA genome and blocking the activity of its genes has emerged. In this work, we presented a database of SARS-CoV-2 targets for small interference RNA (siRNA) based approaches, aiming to speed the design process by providing a broad set of possible targets and siRNA sequences. The siRNAs sequences are characterized and evaluated by more than 170 features, including thermodynamic information, base context, target genes and alignment information of sequences against the human genome, and diverse SARS-CoV-2 strains, to assess possible bindings to off-target sequences. Read More

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New monoclonal antibodies that recognize an unglycosylated, conserved, extracellular region of CD44 in vitro and in vivo, and can block tumorigenesis.

PLoS One 2021 23;16(4):e0250175. Epub 2021 Apr 23.

Developmental Studies Hybridoma Bank, Department of Biology, University of Iowa, Iowa City, Iowa, United States of America.

CD44 is a transmembrane glycoprotein that binds to hyaluronic acid, plays roles in a number of cellular processes and is expressed in a variety of cell types. It is up-regulated in stem cells and cancer. Anti-CD44 monoclonal antibodies affect cell motility and aggregation, and repress tumorigenesis and metastasis. Read More

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sp. nov., isolated from mangrove sediments.

Int J Syst Evol Microbiol 2021 Apr;71(4)

Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, PR China.

A novel Gram-staining-negative, catalase- and oxidase-positive, facultatively anaerobic and rod-shaped motile bacterial strain, designated as ZWAL4003, was isolated from mangrove sediments of the Zini Mangrove Forest, Zhangzhou City, PR China. Phylogenetic analysis based on its 16S rRNA gene sequence indicated that ZWAL4003 was grouped into a separated branch with MSSRF60 (97.38% nucleotide sequence identity) and NBRC 103148 (97. Read More

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sp. nov. and sp. nov., isolated from human faeces.

Int J Syst Evol Microbiol 2021 Apr;71(4)

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, PR China.

Four unknown strains, characterized as Gram-stain-negative, strictly anaerobic, non-motile and rod-shaped, were isolated from fresh faeces of healthy humans in PR China. Pairwise sequence comparisons of the 16S rRNA genes showed that these isolates were separated into two clusters. Cluster I (strains HF-5141 and HF-106) was most closely related to XB1A (98. Read More

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Auditory and visual short-term memory: influence of material type, contour, and musical expertise.

Psychol Res 2021 Apr 21. Epub 2021 Apr 21.

Lyon Neuroscience Research Center (CRNL), INSERM, U1028, CNRS, UMR 5292, Université Claude Bernard Lyon 1, Université de Lyon, Lyon, France.

Short-term memory has mostly been investigated with verbal or visuospatial stimuli and less so with other categories of stimuli. Moreover, the influence of sensory modality has been explored almost solely in the verbal domain. The present study compared visual and auditory short-term memory for different types of materials, aiming to understand whether sensory modality and material type can influence short-term memory performance. Read More

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Detection of mink astrovirus in Poland and further phylogenetic comparison with other European and Canadian astroviruses.

Virus Genes 2021 Apr 15. Epub 2021 Apr 15.

Institute of Biological Basis of Animal Production, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Lublin, Poland.

Mink astrovirus infection remains a poorly understood disease entity, and the aetiological agent itself causes disease with a heterogeneous course, including gastrointestinal and neurological symptoms. This paper presents cases of astrovirus infection in mink from continental Europe. RNA was isolated from the brains and intestines of animals showing symptoms typical of shaking mink syndrome (n = 6). Read More

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First report of northern root-knot nematode, (Chitwood, 1949) on strawberry in Turkey.

J Nematol 2021 25;53. Epub 2021 Feb 25.

Plant Protection Department, Faculty of Agriculture, Atatürk University, 25040, Yakutiye, Erzurum, Turkey.

Strawberry is one of the most economically important crops worldwide. Several species of plant-parasitic nematodes have been reported to be pathogenic on strawberries, among them the northern root-knot nematode (), which considered to be strawberry most important nematode pest worldwide. In August 2019, strawberry growers at Silifke (Mersin, Turkey) identified nematode-like symptoms on strawberry roots and infected seedlings were brought to the nematology laboratory at of Mersin University for diagnostics. Read More

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February 2021

Description of n. sp. (Dorylaimida: Nordiidae) from north Iran.

J Nematol 2021 15;53. Epub 2021 Feb 15.

Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.

This contribution provides the morphological and molecular identification of a new species of the genus (). n. sp. Read More

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February 2021

Morphological and molecular characterization of Goodey, 1929 (Nematoda: Diplogastridae) from South Africa: First report.

J Nematol 2021 26;53. Epub 2021 Mar 26.

Unit for Environmental Sciences and Management, North-West University, Private Bag X6001, Potchefstroom, 2520, South Africa.

Two populations of a species were recovered from compost in two gardens in Potchefstroom, North-West Province, South Africa. Although the genus has previously been reported from South Africa, no species of the genus has ever been identified in the country. Based on morphological, morphometric, and molecular studies, the specimens were identified as and are herein reported for the first time from South Africa. Read More

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The complete chloroplast genome of , an endemic to Europe.

Mitochondrial DNA B Resour 2021 Apr 1;6(4):1300-1302. Epub 2021 Apr 1.

Shandong Institute of Pomology, Shandong Academy of Agricultural Sciences, Taian, PR China.

Sweet chestnut () is an endemic species of genus in Europe, which is widespread in the southern part of continental Europe. The complete genome sequence of chloroplast was determined through Illumina NovaSeq platform. Totally the genome of chloroplast was 160,938 bp in length, GC rich (36. Read More

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Cloning, heterologous expression, and characterization of a novel thioesterase from natural sample.

Heliyon 2021 Mar 24;7(3):e06542. Epub 2021 Mar 24.

Biochemistry Research Group, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Jl. Ganesha 10, Bandung, Indonesia.

A novel thioesterse gene was successfully cloned and sequenced directly from natural sample of Domas Hot Spring, West Java, Indonesia. Homological analysis of the sequence showed that the gene appeared high homology to thioesterase genes with the highest to a putative thioesterase gene from uncultured at 66% identity. However, phylogenetic analysis showed that the protein was separated from the branch with other known thioesterases. Read More

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MRI of the Achilles tendon-A comprehensive pictorial review. Part one.

Eur J Radiol Open 2021 26;8:100342. Epub 2021 Mar 26.

Department of Orthopedics, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Sweden.

The normal Achilles tendon is composed of twisted subtendons separated by thin high signal septae, which are a potential pitfall on MRI because they mimic a tendon tear. Tendinopathy and full thickness tears may be assessed effectively both on MRI and ultrasound. MRI is superior to ultrasound in detection of partial tears and for postoperative assessment. Read More

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The Common Occurrence of Theileria ovis in Tibetan Sheep and the First Report of Theileria sinensis in Yaks from Southern Qinghai, China.

Acta Parasitol 2021 Apr 11. Epub 2021 Apr 11.

National Research Center for Wildlife Borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beichen West Road, Chaoyang District, Beijing, 100101, China.

Purpose: The tick-borne protozoa piroplasms, including Theileria and Babesia, are the cause of substantial economic losses to the livestock industry. However, in southern Qinghai province, China, there are limited information on the molecular characteristics of piroplasms. This study therefore aimed at determining the prevalence and genetic diversity of piroplasms. Read More

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sp. nov., isolated from the pod of soybean, .

Int J Syst Evol Microbiol 2021 Apr;71(4)

Medical Laser Research Center, Dankook University, Cheonan 31116, Republic of Korea.

Soybean pods, separated and enclosed from the outside environment, are considered a suitable place to find new microbes. A Gram-stain-negative, aerobic bacterium, bacterial strain (YB22) was isolated from the pod of (soybean) collected from a rural area in Republic of Korea and characterized by using polyphasic taxonomy. Cells of the strain were rod-shaped (approximately 0. Read More

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