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Single Cell RNA-Seq Analysis Identifies Differentially Expressed Genes of Treg Cell in Early Treatment-Naive Rheumatoid Arthritis By Arsenic Trioxide.

Front Pharmacol 2021 24;12:656124. Epub 2021 May 24.

Department of Rheumatology and Immunology, First Affiliated Hospital of Harbin Medical University, Harbin, China.

Early treatment-naïve rheumatoid arthritis (RA) has defective regulatory T (Treg) cells and increased inflammation response. In this study, we aim to illustrate the regulation of Treg cells in pathogenesis of early rheumatoid arthritis by arsenic trioxide (AsO). We studied the effects of AsO on gene expression in early treatment-naïve RA Treg cells with single cell RNA-seq (scRNA-seq). Read More

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Antigenic Drift of the Hemagglutinin from an Influenza A (H1N1) pdm09 Clinical Isolate Increases its Pathogenicity In Vitro.

Virol Sin 2021 Jun 9. Epub 2021 Jun 9.

State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510180, China.

The influenza A (H1N1) pdm09 virus emerged in 2009 and has been continuously circulating in humans for over ten years. Here, we analyzed a clinical influenza A (H1N1) pdm09-infected patient case hospitalized for two months in Guangdong (from December 14, 2019 to February 15, 2020). This isolate, named A/Guangdong/LCF/2019 (LCF/19), was genetically sequenced, rescued by reverse genetics, and phylogenetically analyzed in the context of other relevant pdm09 isolates. Read More

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Complete mitogenome of the entomopathogenic fungus and phylogenetic analysis of Hypocreales.

Mitochondrial DNA B Resour 2021 May 19;6(6):1689-1690. Epub 2021 May 19.

Institute of Applied Chemistry, Shanxi University, Taiyuan, China.

, with a narrow host range, is an entomopathogenic fungus in the family Clavicipitaceae. Its nuclear genome has been sequenced, whereas its mitogenome is still unknown. In this study, the complete mitogenome of was assembled and annotated. Read More

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The complete plastome of Hedw. (Andreaeaceae, Bryophyta).

Mitochondrial DNA B Resour 2021 May 17;6(5):1656-1657. Epub 2021 May 17.

Bryology Laboratory, School of Life Sciences, East China Normal University, Shanghai, China.

Hedw., one of the lantern mosses, is the lectotype of the genus Hedw. (Andreaeaceae). Read More

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Microbial community structure in hadal sediments: high similarity along trench axes and strong changes along redox gradients.

ISME J 2021 Jun 8. Epub 2021 Jun 8.

Hadal & Nordcee, Department of Biology, University of Southern Denmark, Odense, Denmark.

Hadal trench sediments are hotspots of biogeochemical activity in the deep sea, but the biogeochemical and ecological factors that shape benthic hadal microbial communities remain unknown. Here, we sampled ten hadal sites from two trench regions with a vertical resolution of down to 1 cm. We sequenced 16S rRNA gene amplicons using universal and archaea-specific primer sets and compared the results to biogeochemical parameters. Read More

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Genomic surveillance of in the Philippines, 2013-2014.

Western Pac Surveill Response J 2021 Jan-Mar;12(1):17-25. Epub 2021 Feb 26.

These authors contributed equally to this work.

Antimicrobial-resistant is a major threat to public health and is of particular concern in the Western Pacific Region, where the incidence of gonorrhoea is high. The Antimicrobial Resistance Surveillance Program (ARSP) has been capturing information on resistant gonorrhoea since 1996, but genomic epidemiology studies on this pathogen are lacking in the Philippines. We sequenced the whole genomes of 21 isolates collected in 2013-2014 by ARSP. Read More

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February 2021

SARS-CoV-2 complete genome sequencing from the Italian Campania region using a highly automated next generation sequencing system.

J Transl Med 2021 06 5;19(1):246. Epub 2021 Jun 5.

Cell Biology and Biotherapy Unit, Istituto Nazionale Tumori "Fondazione Giovanni Pascale", IRCCS, Via Mariano Semmola, 80131, Napoli, Italy.

Background: Since the first complete genome sequencing of SARS-CoV-2 in December 2019, more than 550,000 genomes have been submitted into the GISAID database. Sequencing of the SARS-CoV-2 genome might allow identification of variants with increased contagiousness, different clinical patterns and/or different response to vaccines. A highly automated next generation sequencing (NGS)-based method might facilitate an active genomic surveillance of the virus. Read More

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The detection of canine parvovirus type 2c of Asian origin in dogs in Romania evidenced its progressive worldwide diffusion.

BMC Vet Res 2021 Jun 5;17(1):206. Epub 2021 Jun 5.

Department of Veterinary Medical Sciences, Alma Mater Studiorum - University of Bologna, Via Tolara di Sopra 50, 40064, Ozzano dell'Emilia, Bologna, Italy.

Background: Canine parvovirus (CPV) is one of the most important pathogens of dogs. Despite vaccination, CPV infections are still ubiquitous in dogs, and the three antigenic variants 2a, 2b and 2c are variously distributed in the canine population worldwide. To date, no information is available on CPV variants circulating in some European countries. Read More

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Small RNAs in Seminal Plasma as Novel Biomarkers for Germ Cell Tumors.

Cancers (Basel) 2021 May 13;13(10). Epub 2021 May 13.

Department of Growth and Reproduction, Copenhagen University Hospital-Rigshospitalet, 2100 Copenhagen, Denmark.

Circulating miRNAs secreted by testicular germ cell tumors (TGCT) show great potential as novel non-invasive biomarkers for diagnosis of TGCT. Seminal plasma (SP) represents a biofluid closer to the primary site. Here, we investigate whether small RNAs in SP can be used to diagnose men with TGCTs or the precursor lesions, germ cell neoplasia in situ (GCNIS). Read More

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Anti-Vascular Endothelial Growth Factor C Antibodies Efficiently Inhibit the Growth of Experimental Clear Cell Renal Cell Carcinomas.

Cells 2021 May 17;10(5). Epub 2021 May 17.

Département de Biologie Médicale, Centre Scientifique de Monaco, 98000 Monaco, Monaco.

Despite improvement during the last ten years in the longevity of patients with metastatic clear cell renal cell carcinoma (mccRCC) the disease remains incurable. Hence, new therapeutic strategies are urgently needed. Relapse following anti-angiogenic treatment depends on the over-expression of vascular endothelial growth factor C (VEGFC), one of the main drivers of lymphangiogenesis. Read More

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WGS-Based Analysis of Carbapenem-Resistant in Vietnam and Molecular Characterization of Antimicrobial Determinants and MLST in Southeast Asia.

Antibiotics (Basel) 2021 May 11;10(5). Epub 2021 May 11.

Friedrich-Loeffler-Institute, Institute of Bacterial Infections and Zoonoses (IBIZ), Naumburger str.96a, D-07743 Jena, Germany.

Carbapenem-resistant (, CRAb) is an emerging global threat for healthcare systems, particularly in Southeast Asia. Next-generation sequencing (NGS) technology was employed to map genes associated with antimicrobial resistance (AMR) and to identify multilocus sequence types (MLST). Eleven strains isolated from humans in Vietnam were sequenced, and their AMR genes and MLST were compared to published genomes of strains originating from Southeast Asia, i. Read More

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First report of leaf spot disease caused by on American sweetgum ( L.) in China.

Plant Dis 2021 May 31. Epub 2021 May 31.

Nanjing Forestry University, 74584, College of Forestry, No.159, Longpan Road, Xuanwu District, Nanjing, China, 210037.

American sweetgum ( L.) is a forest plant native to North America, which has been introduced into other countries due to its ornamental and medicinal values. In June 2019, symptoms of leaf spots on sweetgum were observed in a field (5 ha) located in Xuzhou, Jiangsu Province, China. Read More

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The immune response and protective efficacy of a potential DNA vaccine against virulent Pasteurella multocida.

J Genet Eng Biotechnol 2021 May 31;19(1):81. Epub 2021 May 31.

Genetics Department, Faculty of Agriculture, Fayoum University, 63514, Fayoum, Egypt.

Background: Pasteurella multocida is the main cause of several infections of farm animals, and the immunity gained from commercial vaccines is for the short term only and needs to be routinely administered, so work on new vaccines against virulent P. multocida is crucial.

Results: In this study, the OmpH gene was amplified from ten P. Read More

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Rapid identification and metagenomics analysis of the adenovirus type 55 outbreak in Hubei using real-time and high-throughput sequencing platforms.

Infect Genet Evol 2021 May 21;93:104939. Epub 2021 May 21.

Chinese PLA Center for Disease Control and Prevention, Beijing, China. Electronic address:

The rise in human adenovirus (HAdV) infections poses a serious challenge to public health in China. Real-time (RT) sequencing provides solutions for achieving rapid pathogen identification during outbreaks, whereas high-throughput sequencing yields higher sequence accuracy. In the present study, we report the outcomes of applying nanopore and BGI platforms in the identification and genomic analysis of an HAdV outbreak in Hubei province, China in May of 2019. Read More

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Occurrence of Leaf Spot of Caused by in China.

Plant Dis 2021 May 24. Epub 2021 May 24.

Wonkwang University, 35030, Department of Food Science and Biotechnology, Iksan, Jeollabuk-do, Korea (the Republic of).

Oats () are an important fodder crop in the vast ranges of northern and northwestern China, given the growing demand from livestock. (Yang et al. 2010). Read More

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The complete plastome of Hedw. (Polytrichaceae, Bryophyta).

Mitochondrial DNA B Resour 2021 May 13;6(5):1645-1647. Epub 2021 May 13.

Bryology Laboratory, School of Life Sciences, East China Normal University, Shanghai, China.

, one of hair-cap mosses, is the type species of the genus Hedw. (Polytrichaceae). Here we present its complete plastome. Read More

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Methylation of TGM-3 Promoter and Its Association with Oral Squamous Cell Carcinoma (OSCC).

Avicenna J Med Biotechnol 2021 Apr-Jun;13(2):65-73

Department of Immunology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Background: Oral Squamous Cell Carcinoma (OSCC) is among the ten most common cancers worldwide. Hypermethylation of CpG sites in the promoter region and subsequent down-regulation of a tumor suppressor gene, TGM-3 has been proposed to be linked to different types of human cancers including OSCC. In this study, methylation status of CpG sites in the promoter region of TGM-3 has been evaluated in a cohort of patients with OSCC compared to normal controls. Read More

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Characteristic and phylogenetic analyses of chloroplast genome for C.K.Schneid. (Oleaceae) from Huoditang, China, an important horticultural plant.

Mitochondrial DNA B Resour 2021 Apr 27;6(4):1521-1522. Epub 2021 Apr 27.

College of Forestry, Northwest A&F University, Yangling, China.

The genus (Oleaceae) comprises some of the most important cultivated horticultural trees worldwide. , is one of these important horticultural plants. We sequenced the complete chloroplast (cp) genomes of (previously identified as var. Read More

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Transmission, characterization and occurrence of recombination in Indian strain of associated with yellow mosaic and leaf curl disease of Summer squash.

3 Biotech 2021 Jun 12;11(6):265. Epub 2021 May 12.

Indian Institute of Horticultural Research, Hessaraghatta Lake PO, Bangalore, Karnataka 560089 India.

Summer squash is one of the important vegetable crops and its production is hampered by various abiotic and biotic stresses. Of the different biotic stresses, viral infections are responsible for causing great losses to this crop. Diseases caused by begomoviruses are becoming a major constraint in the cultivation of summer squash. Read More

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Complete mitochondrial genome characterization and phylogenetic analyses of the main vector of Crimean-Congo haemorrhagic fever virus: Hyalomma marginatum Koch, 1844.

Ticks Tick Borne Dis 2021 May 1;12(5):101736. Epub 2021 May 1.

Department of Parasitology, Faculty of Veterinary Medicine, Erciyes University, Kayseri 38280, Turkey; Vectors and Vector-Borne Diseases Implementation and Research Center, Erciyes University, Kayseri 38280, Turkey.

The Mediterranean tick, Hyalomma marginatum, is the most important vector of Crimean-Congo haemorrhagic fever virus and several pathogens that cause animal and human diseases and economic losses to livestock production. Given the medical and veterinary importance of this tick species, we sequenced and characterized its mitochondrial genome (mitogenome) for the first time. We designed two new primer sets and combined long-range PCR with next generation sequencing to generate complete mitogenomes with deep coverage from 10 H. Read More

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First report of Pythium aphanidermatum causing root rot of head lettuce in China.

Plant Dis 2021 May 13. Epub 2021 May 13.

Shandong Academy of Agricultural Sciences, Institute of Plant Protection, NO.202,Industrial north road,Licheng district, Jinan, Shandong, China, 250100;

Head lettuce (Lactuca sativa L.) is an important crop for fresh consumption in China. In Shandong Province, head lettuce is planted in spring and in autumn each year. Read More

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Sindbis virus infection of mosquito species in the wetlands of northwestern Iran and modeling the probable ecological niches of SINV vectors in the country.

Acta Trop 2021 May 9;220:105952. Epub 2021 May 9.

Department of Medical Entomology and Vector Control, School of Public Health, Urmia University of Medical Sciences, Urmia, Iran; Social Determinants of Health, Research Center, Urmia University of Medical Sciences, Urmia, Iran. Electronic address:

Sindbis virus (SINV) and Chikungunya virus (CHIKV) are among the most widely spread mosquito-borne viruses worldwide. Due to the key role of mosquitoes in the transmission cycle of vector-borne diseases, models such as Maximum Entropy (MaxEnt) have been used in recent years to predict the environmental suitability and ecological niches of mosquito vectors. Infection of three mosquito species (Anopheles maculipennis s. Read More

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Integrated analysis of three newly sequenced fern chloroplast genomes: Genome structure and comparative analysis.

Ecol Evol 2021 May 18;11(9):4550-4563. Epub 2021 Mar 18.

Department of Ecology Institute of Natural Resources and Ecology Heilongjiang Academy of Science Harbin China.

Background: Some ferns have medicinal properties and are used in therapeutic interventions. However, the classification and phylogenetic relationships of ferns remain incompletely reported. Considering that chloroplast genomes provide ideal information for species identification and evolution, in this study, three unpublished and one published ferns were sequenced and compared with other ferns to obtain comprehensive information on their classification and evolution. Read More

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First report of causing root rot and seedling elongation of soybean in Indiana.

Plant Dis 2021 May 11. Epub 2021 May 11.

USDA/ARS, Crop Production and Pest Control Research Unit, Lilly B462A, 915 West State Street, West Lafayette, Indiana, United States, 47907.

In summer 2020, 127 soybean (Glycine max (L.) Merr) seedlings (V1-V3 stage) showing reduced vigor or crown lesions were collected at Purdue's Agronomy Center for Research and Education in West Lafayette, Indiana. Root tissues from two seedlings with necrotic cotyledons and root rot were surface-sterilized and plated on dichloran-chloramphenicol-peptone agar (Andrews and Pitt 1986). Read More

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First report of a ' Phytoplasma aurantifolia'-related strain (16SrII-V) associated with phyllody, virescence, and shoot proliferation of sweet William () in Taiwan.

Plant Dis 2021 May 11. Epub 2021 May 11.

National Chung Hsing University, 34916, Department of Plant Pathology, Taichung, Taiwan.

The sweet William () is an ornamental belonging to the Caryophyllaceae family; the species produces clusters of flowers that comes in various colors and is grown commonly as garden plants (Lim 2014). In February 2021, sweet Williams showing symptoms typical of phytoplasma diseases were found in a garden located in Wufeng District, Taichung, Taiwan (24°04'37.6"N 120°43'20. Read More

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First Report of Alfalfa Anthracnose Caused by in Xinjiang, China.

Plant Dis 2021 May 10. Epub 2021 May 10.

Bazhou Agricultural Technology Promotion Center, plant protection , korla, xinjiang, China;

Alfalfa ( L.) is widely planted in the world as one of the most important leguminous forage crops, and it is also the first choice of forage crops for animal feed in Xinjiang. In June 2018, alfalfa plants with typical anthracnose symptoms were observed in 75% of alfalfa fields in Hutubi County, Xinjiang, China. Read More

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First Report of Causing Anthracnose of Guava () in Mexico.

Plant Dis 2021 May 10. Epub 2021 May 10.

Centro de Investigación en Alimentación y Desarrollo, Coordinación Culiacán, Carretera El Dorado Km 5.5, Campo el Diez, Culiacán, Sinaloa, Mexico, 80110;

Guava ( L.) is a small tree belonging to the Myrtaceae family and it is distributed worldwide in the tropical and subtropical areas. During the summer of 2019, symptoms of fruit anthracnose were observed on approx. Read More

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First Report of Pantoea ananatis causing leaf spot disease of maize in Ecuador.

Plant Dis 2021 May 10. Epub 2021 May 10.

Universidad UTE , Centro de Investigación de Alimentos, Quito, Pichincha, Ecuador;

Maize (Zea mays) is the second most cultivated grain crop in Ecuador, with growing significance as a source of fodder and food. During the rainy season (November and December) of 2018 and 2019, a disease of maize that was not previously observed in Ecuador was found at commercial fields of Misqui Sara variety, at four parishes of canton Quito (Tumbaco, Pifo, Puembo, and Checa), province of Pichincha. Infected plants, at tassel initiation, displayed symptoms of localized chlorotic streaks on leaves that expanded with time, and around a month later turned necrotic. Read More

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