1,413 results match your criteria sequence grapevine

Evidence of differential spreading events of grapevine pinot Gris virus in Italy using datamining as a tool.

Eur J Plant Pathol 2021 Aug 26:1-8. Epub 2021 Aug 26.

Université de Strasbourg, INRAE, SVQV UMR-A 1131, F-68000 Colmar, France.

Since its identification in 2003, grapevine Pinot gris virus (GPGV, ) has now been detected in most grape-growing countries. So far, little is known about the epidemiology of this newly emerging virus. In this work, we used datamining as a tool to monitor the sanitary status of three vineyards in Italy. Read More

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Novel collophorina-like genera and species from trees and vineyards in Germany.

S Bien C Kraus U Damm

Persoonia 2020 Dec 10;45:46-67. Epub 2019 Sep 10.

Senckenberg Museum of Natural History Görlitz, Department Botany, Section Mycology, PF 300 154, 02806 Görlitz, Germany.

Strains with a yeast-like appearance were frequently collected in two surveys on the biodiversity of fungi in Germany, either associated with necroses in wood of trees in orchards in Saxony, Lower Saxony and Baden-Württemberg or captured in spore traps mounted on grapevine shoots in a vineyard in Rhineland-Palatinate. The morphology of the strains was reminiscent of the genus : all strains produced aseptate conidia on integrated conidiogenous cells directly on hyphae, on discrete phialides, adelophialides and by microcyclic conidiation, while in some strains additionally endoconidia or conidia in conidiomata were observed. Blastn searches with the ITS region placed the strains in the close to spp. Read More

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December 2020

Seeing the Forest through the (Phylogenetic) Trees: Functional Characterisation of Grapevine Terpene Synthase () Paralogues and Orthologues.

Plants (Basel) 2021 Jul 26;10(8). Epub 2021 Jul 26.

Department of Viticulture and Oenology, Faculty of AgriSciences, South African Grape and Wine Research Institute, Stellenbosch University, Stellenbosch 7600, South Africa.

Gene families involved in specialised metabolism play a key role in a myriad of ecophysiological and biochemical functions. The sesquiterpene synthases represent the largest subfamily of grapevine terpene synthase () genes and are important volatile metabolites for wine flavour and aroma, as well as ecophysiological interactions. The functional characterisation of genes is complicated by a reliance on a single reference genome that greatly underrepresents this large gene family, exacerbated by extensive duplications and paralogy. Read More

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Genome Sequence Resource for Erysiphe necator NAFU1, a Grapevine Powdery Mildew Isolate Identified in Shaanxi Province of China.

Mol Plant Microbe Interact 2021 Aug 26. Epub 2021 Aug 26.

Northwest A&F University, 12469, State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Yangling, Shaanxi, China.

Erysiphe necator is an economically important biotrophic fungal pathogen responsible for powdery mildew disease on grapevine. Currently, genome sequences are available for only a few Erysiphe necator isolates from USA. Based on the combination of Nanopore and Illumina sequencing technologies, we present here the complete genome assembly for an isolate of E. Read More

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First report of Grapevine enamovirus 1 in Vitis vinifera cultivar 'Meunier' in France.

Plant Dis 2021 Aug 22. Epub 2021 Aug 22.

Université de Strasbourg, INRAE, SVQV UMR-A 1131, F-68000 Colmar, France, Colmar, France;

Grapevine enamovirus 1 (GEV-1) is a member of the genus Enamovirus in the family Solemoviridae. GEV-1 was first described in 2017 in a few grapevine cultivars in Brazil (Silva et al. 2017) and subsequently in China (Ren et al. Read More

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High-Throughput Sequencing Indicates a Novel Marafivirus in Grapevine Showing Vein-Clearing Symptoms.

Plants (Basel) 2021 Jul 20;10(7). Epub 2021 Jul 20.

National Center for Eliminating Viruses from Deciduous Fruit Trees, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng 125100, China.

A putative new marafivirus was identified in a 'Jumeigui' grapevine exhibitting obvious vein-clearing symptoms by high-throughput sequencing, which tentatively named grapevine-associated marafivirus (GaMV). The nearly complete genomic sequence of GaMV was amplified by reverse transcription PCR, and the terminal sequences were determined using the rapid amplification of cDNA ends method. The nearly complete genome of GaMV is 6346 bp long, excluding the poly(A) tail, and shows 51. Read More

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Metagenomic analysis of nepoviruses: diversity, evolution and identification of a genome region in members of subgroup A that appears to be important for host range.

Arch Virol 2021 Oct 9;166(10):2789-2801. Epub 2021 Aug 9.

Université de Strasbourg, INRAE, SVQV, UMR-A 1131, F-68000, Colmar, France.

Data mining and metagenomic analysis of 277 open reading frame sequences of bipartite RNA viruses of the genus Nepovirus, family Secoviridae, were performed, documenting how challenging it can be to unequivocally assign a virus to a particular species, especially those in subgroups A and C, based on some of the currently adopted taxonomic demarcation criteria. This work suggests a possible need for their amendment to accommodate pangenome information. In addition, we revealed a host-dependent structure of arabis mosaic virus (ArMV) populations at a cladistic level and confirmed a phylogeographic structure of grapevine fanleaf virus (GFLV) populations. Read More

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October 2021

Sequence and Gene Expression Analysis of Recently Identified NLP from .

Microorganisms 2021 Jul 6;9(7). Epub 2021 Jul 6.

Department of Biology, State Institute of Viticulture and Enology, 79100 Freiburg, Germany.

Grapevine downy mildew, evoked by the obligate biotrophic oomycete is one of the most challenging diseases in viticulture. establishes an infection by circumvention of plant immunity, which is achieved by the secretion of effector molecules. One family of potential effectors are the necrosis- and ethylene-inducing peptide 1 (Nep1)-like proteins (NLP). Read More

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Conservation of endo-glucanase 16 (EG16) activity across highly divergent plant lineages.

Biochem J 2021 Aug;478(16):3063-3078

Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, BC Canada V6T 1Z1.

Plant cell walls are highly dynamic structures that are composed predominately of polysaccharides. As such, endogenous carbohydrate active enzymes (CAZymes) are central to the synthesis and subsequent modification of plant cells during morphogenesis. The endo-glucanase 16 (EG16) members constitute a distinct group of plant CAZymes, angiosperm orthologs of which were recently shown to have dual β-glucan/xyloglucan hydrolase activity. Read More

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First report of grapevine rupestris vein feathering virus in L. from Japan.

Plant Dis 2021 Jul 7. Epub 2021 Jul 7.

University of Yamanashi, 38146, The Institute of Enology and Viticulture, Kofu, Yamanashi, Japan;

The production of wine grapes is gaining widespread popularity and being carried out on approximately 2,200 hectares of land in Japan. Scions grafted onto rootstocks generally have been imported from the EU, USA, New Zealand, and Australia into Japan. Unfortunately, viruses have spread in Japanese vineyards by slipping through the net of plant quarantine. Read More

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First Report of Grapevine Kizil Sapak Virus in Grapevine in China.

Plant Dis 2021 Jul 6. Epub 2021 Jul 6.

National Center for Eliminating Viruses from Deciduous Fruit Tree, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng, China;

Grapevine Kizil Sapak virus (GKSV) is a novel member of the family Betaflexiviridae classified into the proposed genus Fivivirus within the subfamily Trivirinae. It was first discovered in USA from a grapevine originating from Turkmenistan (Al Rwahnih et al. 2019) and later in France from a grapevine accession from Iran (Marais et al. Read More

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Evaluation of Sown Cover Crops and Spontaneous Weed Flora as a Potential Reservoir of Black-Foot Pathogens in Organic Viticulture.

Biology (Basel) 2021 Jun 3;10(6). Epub 2021 Jun 3.

Instituto Agroforestal Mediterráneo, Universitat Politècnica de València, Camino de Vera S/N, 46022 Valencia, Spain.

(1) Background. An extensive survey of grapevine-sown cover crops and spontaneous weed flora was conducted from 2019 to 2020 in organic vineyards in six European countries (France, Italy, Romania, Slovenia, Spain, Switzerland). Our main objective was to detect and identify the presence of -like asexual morphs species associated with black-foot disease on their roots. Read More

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Variable Populations of Grapevine Virus T Are Present in Vineyards of Hungary.

Viruses 2021 06 10;13(6). Epub 2021 Jun 10.

Institute of Plant Protection, Hungarian University of Agriculture and Life Sciences, Ménesi Road 44, H-1118 Budapest, Hungary.

Grapevine virus T (GVT) is a recently described foveavirus, which was identified from a transcriptome of a Teroldego grapevine cultivar in 2017. Recently, we surveyed vineyards and rootstock plantations in Hungary using small RNA (sRNA) high-throughput sequencing (HTS), at a time when GVT had not yet been described. A re-analysis of our sRNA HTS datasets and a survey of grapevines by RT-PCR revealed the presence of GVT in most of the vineyards tested, while at rootstock fields its presence was very rare. Read More

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A novel ampelovirus associated with mealybug wilt of pineapple (Ananas comosus).

Virus Genes 2021 Oct 28;57(5):464-468. Epub 2021 Jun 28.

Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI, USA.

Mealybug wilt of pineapple (MWP) is the most important and complex viral disease affecting pineapple worldwide. High-throughput sequencing was conducted to characterize a new virus identified only in symptomatic pineapple plants and tentatively named pineapple mealybug wilt-associated virus 6 (PMWaV-6). Data analyses revealed a genome of 17,854 nucleotides with an organization resembling members of the genus Ampelovirus, family Closteroviridae. Read More

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October 2021

Small RNA and transcriptome sequencing of a symptomatic peony plant reveals mixed infections with novel viruses.

Plant Dis 2021 Jun 22. Epub 2021 Jun 22.

Shang Qiao Zhong Lu, Chaoyang DistrictBeijing, China, 100024.

To identify the viruses in tree peony plants associated with the symptoms of yellowing, leaf rolling, stunted growth, and decline, high-throughput sequencing of small RNA and mRNA was conducted from a single symptomatic plant. Bioinformatic analyses and reconstruction of viral genomes indicated mixed viral infections involving cycas necrotic stunt virus (CNSV), apple stem grooving virus (ASGV), lychnis mottle virus (LycMoV), grapevine line pattern virus (GLPV), and three new viruses designated as peony yellowing-associated citrivirus (PYaCV, Citrivirus in Betaflexiviridae), peony betaflexivirus 1 (PeV1, unclassified in Betaflexiviridae), and peony leafroll-associated virus (PLRaV, Ampelovirus in Closteroviridae). PYaCV was 8,666 nucleaotides (nt) in length, comprising three open reading frames (ORFs) and shared 63. Read More

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Polymorphisms at the 3'end of the movement protein (MP) gene of grapevine Pinot gris virus (GPGV) affect virus titre and small interfering RNA accumulation in GLMD disease.

Virus Res 2021 Sep 11;302:198482. Epub 2021 Jun 11.

Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine 33100, Italy; Istituto Nazionale Biostrutture e Biosistemi, Rome, Italy. Electronic address:

Grapevine Leaf Mottling and Deformation (GLMD) is a grapevine disease that has been associated with a trichovirus, the grapevine Pinot gris virus (GPGV). A wide diversity in the severity of GLMD disease symptoms has been recorded worldwide, but the relationship of this diversity to the sequence variation in the GPGV genome is still a matter of debate. Results from comparative analysis of GPGV genomic sequences have suggested an association of polymorphisms at the 3'-end of the movement protein (MP) with GLMD severity. Read More

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September 2021

Cadophora sabaouae sp. nov. and Phaeoacremonium species associated with Petri disease on grapevine propagation material and young grapevines in Algeria.

Plant Dis 2021 Jun 7. Epub 2021 Jun 7.

Mendelova univerzita v Brne, 48269, MENDELEUM - Institute of Genetics, Mendel University in Brno, Faculty of Horticulture, Mendeleum - Institute of Genetics, Valticka 334, 69144, Lednice, Czech Republic, Brno, Czech Republic, 69144;

A field survey conducted on asymptomatic grapevine propagation material from nurseries and symptomatic young grapevines throughout different regions of Algeria yielded a collection of 70 Phaeoacremonium-like isolates and three Cadophora-like isolates. Based on morphology and DNA sequence data of β-tubulin (tub2) and actin (act), five Phaeoacremonium species were identified including Phaeoacremonium minimum (22 isolates), P. venezuelense (19 isolates), P. Read More

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First report of Grapevine yellow speckle viroid 1 infecting grapevine (Vitis vinifera L.) in Canada.

Plant Dis 2021 Jun 7. Epub 2021 Jun 7.

Agriculture and AgriFood Canada , Research and development center , 430 Gouin, Saint-Jean-sur-Richelieu, Quebec, Canada, J3B3E6;

Quebec is the third largest wine grape producer in Canada in acreage, tonnage, and wine grape sales (Carisse et al. 2017; Ben Moussa et al. 2019). Read More

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Current status and future prospects of grapevine anthracnose caused by Elsinoe ampelina: An important disease in humid grape-growing regions.

Mol Plant Pathol 2021 Aug 7;22(8):899-910. Epub 2021 Jun 7.

State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, China.

Anthracnose, caused by Elsinoe ampelina, is one of the most destructive diseases of grapevines worldwide, especially in humid areas. E. ampelina mainly infects young tissues starting from shoots to berries and affects vine vigour and berry yield. Read More

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Complete genome sequence of a novel citrus virus with characteristics of members of the family Tymoviridae.

Arch Virol 2021 Jul 5;166(7):2055-2058. Epub 2021 May 5.

National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing, 400712, China.

A novel positive-stranded RNA virus provisionally named "citrus virus C" (CVC) was discovered in citrus trees displaying mottling symptoms. Its genome comprises 7,215 nucleotides (nt), excluding the 3' poly(A) tail, and contains two open reading frames (ORFs) that encode a replication-associated polyprotein (RP) and a putative coat protein (CP). The CVC genome contains a 16-nt 'marafibox', which is highly conserved in most viruses belonging to the genus Marafivirus of the same family. Read More

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Comparison of Short-Read Sequence Aligners Indicates Strengths and Weaknesses for Biologists to Consider.

Front Plant Sci 2021 16;12:657240. Epub 2021 Apr 16.

Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester, NY, United States.

Aligning short-read sequences is the foundational step to most genomic and transcriptomic analyses, but not all tools perform equally, and choosing among the growing body of available tools can be daunting. Here, in order to increase awareness in the research community, we discuss the merits of common algorithms and programs in a way that should be approachable to biologists with limited experience in bioinformatics. We will only in passing consider the effects of data cleanup, a precursor analysis to most alignment tools, and no consideration will be given to downstream processing of the aligned fragments. Read More

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First report of grapevine rupestris vein feathering virus in wine grapes in Idaho.

Plant Dis 2021 May 2. Epub 2021 May 2.

University of Idaho, EPPN, 875 Perimeter Dr., MS 2329, Moscow, Idaho, United States, 83844-2329;

Grapevine rupestris vein feathering virus (GRVFV) was found associated with chlorotic discolorations of leaf veins in a Greek grapevine cultivar (El Beaino et al. 2001; Abou Ghanem-Sabanadzovic et al. 2003) or with Syrah decline (Al Rwahnih et al. Read More

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Genome sequence analysis of the beneficial Bacillus subtilis PTA-271 isolated from a Vitis vinifera (cv. Chardonnay) rhizospheric soil: assets for sustainable biocontrol.

Environ Microbiome 2021 Jan 29;16(1). Epub 2021 Jan 29.

SFR Condorcet - FR CNRS 3417, University of Reims Champagne-Ardenne, Induced Resistance and Plant Bioprotection (RIBP) - EA 4707, BP1039, Cedex 2, F-51687, Reims, France.

Background: Bacillus subtilis strains have been widely studied for their numerous benefits in agriculture, including viticulture. Providing several assets, B. subtilis spp. Read More

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January 2021

Complete genome sequence of a grapevine Roditis leaf discoloration-associated virus (GRLDaV) variant from South Africa.

Arch Virol 2021 Jul 26;166(7):2041-2044. Epub 2021 Apr 26.

Department of Genetics, Stellenbosch University, Stellenbosch, 7600, South Africa.

High-throughput sequencing (HTS) was used to construct the virome profile of an old grapevine-leafroll-diseased grapevine (Vitis vinifera). De novo assembly of HTS data showed a complex infection, including a virus sequence with similarity to viruses of the genus Badnavirus, family Caulimoviridae. The complete genome sequence of this virus consists of 7090 nucleotides and has four open reading frames (ORFs). Read More

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Characterization of Genes From : Searching for the Most Virulent Ones.

Front Microbiol 2021 22;12:632047. Epub 2021 Mar 22.

State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, China.

Grapevine downy mildew is an insurmountable disease that endangers grapevine production and the wine industry worldwide. The causal agent of the disease is the obligate biotrophic oomycete , for which the pathogenic mechanism remains largely unknown. Crinkling and necrosis proteins (CRN) are an ancient class of effectors utilized by pathogens, including oomycetes, that interfere with host plant defense reactions. Read More

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A key 'foxy' aroma gene is regulated by homology-induced promoter indels in the iconic juice grape 'Concord'.

Hortic Res 2020 Apr 18;7(1):67. Epub 2020 Apr 18.

US Department of Agriculture-Agricultural Research Service, Grape Genetics Research Unit, Geneva, NY, USA.

'Concord', the most well-known juice grape with a parentage of the North American grape species Vitis labrusca L., possesses a special 'foxy' aroma predominantly resulted from the accumulation of methyl anthranilate (MA) in berries. This aroma, however, is often perceived as an undesirable attribute by wine consumers and rarely noticeable in the common table and wine grape species V. Read More

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First report of Flavescence dorée-related Phytoplasma in Grapevine in Germany.

Plant Dis 2021 Apr 6. Epub 2021 Apr 6.

Julius Kühn-Institut (JKI) Federal Research Institute for Cultivated Plants, Siebeldingen, Germany;

Flavescence dorée (FD) and Bois noir (BN) are the principal grapevine yellows diseases in Europe caused by distinct phytoplasmas: FD by 16SrV phytoplasmas (FDp), BN by Candidatus Phytoplasma solani. FDp is spread epidemically by the introduced Nearctic Deltocephalinae Scaphoideus titanus and is listed as a quarantine pest in the European Union (Regulation (EU) 2019/2072). Black Alder (Alnus glutinosa) is a common asymptotic host of 16SrV phytoplasmas in Europe and considered the original host of FDp (Malembic-Maher et al. Read More

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Probing into the Effects of Grapevine Leafroll-Associated Viruses on the Physiology, Fruit Quality and Gene Expression of Grapes.

Viruses 2021 03 31;13(4). Epub 2021 Mar 31.

Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada.

Grapevine leafroll is one of the most widespread and highly destructive grapevine diseases that is responsible for great economic losses to the grape and wine industries throughout the world. Six distinct viruses have been implicated in this disease complex. They belong to three genera, all in the family Closteroviridae. Read More

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Differential Response of Grapevine to Infection with ' Phytoplasma solani' in Early and Late Growing Season through Complex Regulation of mRNA and Small RNA Transcriptomes.

Int J Mol Sci 2021 Mar 29;22(7). Epub 2021 Mar 29.

National Institute of Biology, 1000 Ljubljana, Slovenia.

Bois noir is the most widespread phytoplasma grapevine disease in Europe. It is associated with ' Phytoplasma solani', but molecular interactions between the causal pathogen and its host plant are not well understood. In this work, we combined the analysis of high-throughput RNA-Seq and sRNA-Seq data with interaction network analysis for finding new cross-talks among pathways involved in infection of grapevine cv. Read More

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Genetic Diversity of in Mexican Vineyards.

Plant Dis 2021 May 29;105(5):1490-1494. Epub 2021 Mar 29.

Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Av. IPN 2508 San Pedro Zacatenco, 07360 CDMX, México.

is a xylem-inhabiting phytopathogenic bacterium that affects diverse agriculturally relevant crops. In Mexico, has been reported in the states of Baja California, Coahuila, and Querétaro. In order to determine the genetic diversity of this bacterium in Mexico, 408 grapevine samples were collected from the main producing states in México. Read More

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